Shogo Higaki, Tomomi Nishie, Takaaki Todo, Reiko Teshima, Kenichiro Kusumi, Risa Mitsumori, Ikuo Tooyama, Yasuhiro Fujioka, Toshihiro Kawasaki, Noriyoshi Sakai, Tatsuyuki Takada
Fishes expressing a fluorescent protein in germ cells are useful to perform germ cell transfer experiments for conservation study. However, no such fish has been generated in endangered endemic fishes. In this study, we tried to generate a fish expressing Venus fluorescent protein in germ cells using Honmoroko (Gnathopogon caerulescens), which is one of the threatened small cyprinid endemic to ancient Lake Biwa in Japan. To achieve germ cell-specific expression of Venus, we used piwil1 (formally known as ziwi) promoter and Tol2 transposon system...
June 29, 2021: Journal of Fish Biology