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Věra Čertíková Chábová, Petr Kujal, Petra Škaroupková, Zdeňka Varňourková, Šárka Vacková, Zuzana Husková, Soňa Kikerlová, Janusz Sadowski, Elzbieta Kompanowska-Jezierska, Iwona Baranowska, Sung Hee Hwang, Bruce D Hammock, John D Imig, Vladimír Tesař, Ludek Červenka
BACKGROUND/AIMS: We found recently that increasing renal epoxyeicosatrienoic acids (EETs) levels by blocking soluble epoxide hydrolase (sEH), an enzyme responsible for EETs degradation, shows renoprotective actions and retards the progression of chronic kidney disease (CKD) in Ren-2 transgenic hypertensive rats (TGR) after 5/6 renal ablation (5/6 NX). This prompted us to examine if additional protection is provided when sEH inhibitor is added to the standard renin-angiotensin system (RAS) blockade, specifically in rats with established CKD...
March 6, 2018: Kidney & Blood Pressure Research
P Kala, L Sedláková, P Škaroupková, L Kopkan, Z Vaňourková, M Táborský, A Nishiyama, S H Hwang, B D Hammock, J Sadowski, V Melenovský, J D Imig, L Červenka
We showed recently that increasing kidney epoxyeicosatrienoic acids (EETs) by blocking soluble epoxide hydrolase (sEH), an enzyme responsible for EETs degradation, retarded the development of renal dysfunction and progression of aorto-caval fistula(ACF)-induced congestive heart failure (CHF) in Ren-2 transgenic hypertensive rats (TGR). In that study the final survival rate of untreated ACF TGR was only 14 % but increased to 41 % after sEH blockade. Here we examined if sEH inhibition added to renin-angiotensin system (RAS) blockade would further enhance protection against ACF-induced CHF in TGR...
March 12, 2018: Physiological Research
Elshin Mathias, Allanah Kenny, Michael J Plank, Tim David
A state-of-the-art integrated model of neurovascular coupling (NVC) [19, 18, 38] and the BOLD response [47, 48] is presented with the ability to simulate the fMRI BOLD responses due to continuous neuronal spiking, bursting and cortical spreading depression (CSD) along with the underlying complex vascular coupling. Simulated BOLD responses are compared to experimental BOLD signals observed in the rat barrel cortex and in the hippocampus under seizure conditions showing good agreement. Bursting phenomena provides relatively clear BOLD signals as long as the time between bursts is not too short...
March 8, 2018: NeuroImage
Vasundhara Kain, Kevin A Ingle, Janusz Kabarowski, Stephen Barnes, Nita A Limdi, Sumanth D Prabhu, Ganesh V Halade
12/15 lipoxygenase (LOX) directs inflammation and lipid remodeling. However, the role of 12/15LOX in post-myocardial infarction (MI) left ventricular remodeling is unclear. To determine the role of 12/15LOX, 8-12 week-old C57BL/6 J wild-type (WT; n = 93) and 12/15LOX-/- (n = 97) mice were subjected to permanent coronary artery ligation and monitored at day (d)1 and d5 post-operatively. Post-MI d28 survival was measured in male and female mice. No-MI surgery mice were maintained as d0 naïve controls...
March 8, 2018: Journal of Molecular and Cellular Cardiology
Arun Bahadur Gurung, Bishwarjit Mayengbam, Atanu Bhattacharjee
Soluble epoxide hydrolase (sEH), a key enzyme belonging to cytochrome P450 pathway of arachidonic acid cascade is a novel therapeutic drug target against atherosclerosis. The enzyme breaks down epoxyeicosatrienoic acid (EETs) to dihydroxy-eicosatrienoic acids (DHETs) and reduces beneficial cardiovascular properties of EETs. Thus, the present work is aimed at identification of potential leads as sEH inhibitors which will sustain the beneficial properties of EETs in vivo. PubChem and ZINC databases were screened for drug-like compounds based on Lipinski's rule of five and in silico toxicity filters...
March 6, 2018: Computational Biology and Chemistry
Wenshan Lv, George W Booz, Fan Fan, Yangang Wang, Richard J Roman
Chronic kidney disease (CKD) is a significant worldwide healthcare problem. Regardless of the initial injury, renal fibrosis is the common final pathway leading to end stage renal disease. Although the underlying mechanisms are not fully defined, evidence indicates that besides inflammation, oxidative stress plays a crucial role in the etiology of renal fibrosis. Oxidative stress results from an imbalance between the production of free radicals that are often increased by inflammation and mitochondrial dysfunction, and reduced anti-oxidant defenses...
2018: Frontiers in Physiology
Man Chen, Xiaofang Zhou, Xing Liu, Raymond Jianxiong Zeng, Fang Zhang, Jie Ye, Shungui Zhou
The conductivity of a biofilm is the key factor for the high current density of a bioelectrochemical system (BES). Most previous works have focused on electrode modification, but, this only benefits the microorganisms that directly contact the electrode. The low conductivity of biofilm limits the current density of the BES. In this work, gold nanoparticles (Au-NPs) were successfully fabricated in situ into a Geobacter sulfurreducens biofilm to increase the conductivity. 20 ppm NaAuCl4 (the precursor) was slowly dropped into the anode chamber at a rate of 1...
February 12, 2018: Biosensors & Bioelectronics
Hyung-Sool Lee
This review explores electron transfer kinetics from an electron donor to the anode in electrically conductive biofilm anodes. Intracellular electron transfer (IET) from the donor to the anode is well described with the Monod equation. In comparison, mechanisms of extracellular electron transfer (EET) conduction are unclear yet, complicating EET kinetics. However, in biofilm anodes where potential gradient to saturated current density is less than ∼300 mV, Ohmic conduction successfully describe conductive EET mainly with biofilm conductivity (Kbio ) and biofilm thickness (Lf )...
February 22, 2018: Bioresource Technology
Nguyen Thanh Phuc, Akihito Ishizaki
Excitation energy transfer (EET) is one of the most important processes in both natural and artificial chemical systems including, for example, photosynthetic complexes and organic solar cells. The EET rate, however, is strongly suppressed when there is a large difference in the excitation energy between the donor and acceptor molecules. Here, we demonstrate both analytically and numerically that the EET rate can be greatly enhanced by periodically modulating the excitation energy difference. The enhancement of EET by using this Floquet engineering, in which the system's Hamiltonian is made periodically time-dependent, turns out to be efficient even in the presence of strong fluctuations and dissipations induced by the coupling with a huge number of dynamic degrees of freedom in the surrounding molecular environments...
February 22, 2018: Journal of Physical Chemistry Letters
Satoshi Kawaichi, Tetsuya Yamada, Akio Umezawa, Shawn E McGlynn, Takehiro Suzuki, Naoshi Dohmae, Takashi Yoshida, Yoshihiko Sako, Nobuhiro Matsushita, Kazuhito Hashimoto, Ryuhei Nakamura
Ardenticatena maritima strain 110S is a filamentous bacterium isolated from an iron-rich coastal hydrothermal field, and it is a unique isolate capable of dissimilatory iron or nitrate reduction among the members of the bacterial phylum Chloroflexi . Here, we report the ability of A. maritima strain 110S to utilize electrodes as a sole electron acceptor and donor when coupled with the oxidation of organic compounds and nitrate reduction, respectively. In addition, multicellular filaments with hundreds of cells arranged end-to-end increased the extracellular electron transfer (EET) ability to electrodes by organizing filaments into bundled structures, with the aid of microbially reduced iron oxide minerals on the cell surface of strain 110S...
2018: Frontiers in Microbiology
Qi Zhang, Xiang Wang, Guifang Yan, Juan Lei, Yu Zhou, Lei Wu, Ting Wang, Xiao Zhang, Duyun Ye, Yongsheng Li
BACKGROUND/AIMS: This study aimed to explore the metabololipidome in mice upon cupping treatment. METHODS: A nude mouse model mimicking the cupping treatment in humans was established by administrating four cupping sets on the back skin for 15 minutes. UPLC-MS/ MS was performed to determine the PUFA metabolome in mice skin and blood before and after cupping treatment. The significantly changed lipids were administered in macrophages to assess the production of pro-inflammatory cytokines IL-6 and TNF-α by ELISA...
2018: Cellular Physiology and Biochemistry
Haruto Yamanashi, William E Boeglin, Christophe Morisseau, Robert W Davis, Gary A Sulikowski, Bruce D Hammock, Alan R Brash
Lipoxygenase-catalyzed oxidation of the essential fatty acid linoleate represents a vital step in construction of the mammalian epidermal permeability barrier. Analysis of epidermal lipids indicates the linoleate is converted to a trihydroxy derivative by hydrolysis of an epoxy-hydroxy precursor. We evaluated different epoxide hydrolase (EH) enzymes in the hydrolysis of skin-relevant fatty acid epoxides and compared the products to those of acid-catalyzed hydrolysis. In the absence of enzyme, exposure to pH 5 or pH 6 at 37 deg C for 30 min hydrolyzed fatty acid allylic epoxyalcohols to four trihydroxy products...
February 19, 2018: Journal of Lipid Research
Srinadh Komanduri, V Raman Muthusamy, Sachin Wani
Advances in Endoscopic Eradication Therapy for Barrett's Esophagus associated neoplasia have resulted in a significant paradigm shift in diagnosis and management of this complex disease. A robust body of literature critically evaluating outcomes of resection and ablative strategies has allowed gastroenterologists to make quality, evidence based decisions for their patients. Despite this progress, there are still many unanswered questions and challenges that remain. Ultimately, identification of a cost-effective screening modality, biomarkers for risk stratification, and strides to eliminate post surveillance endoscopy after EET are essential to reach our long term goal for eradication of esophageal adenocarcinoma...
February 16, 2018: Gastroenterology
Albert Collins Nganou Assonkeng, Noam Adir, Martin Mkandawire
Understanding of electronic energy transition (EET) mechanisms from the light-harvesting unit to the reaction centre in a natural system has been limited by; (a) the use of conventional transient time-resolved spectroscopy at room temperature, which result in high signal-to-noise ratio; and (b) examining extracts instead of intact light-harvesting units. Here, we report previously unknown differences and new insight in EET of two cyanobacteria species, Acaryochloris marina and Thermosynechoccocus vulvanus, which have been found only after using Uv-vis, hole-burning and fluorescence spectroscopy at ultra-low temperature and examining their intact light-harvesting unit, phycobilisomes (PBS)...
February 19, 2018: Journal of Physical Chemistry. B
Amardeep Singh, Sarvpreet Singh Grewal, Navneet Kumar
To analyze the clinical presentation, management and outcomes of patients with pituitary adenoma treated by Endoscopic Endo-nasal Trans-sphenoidal (EETS) excision. Study was conducted on the basis of medical records of 14 patients who had undergone EETS excision of pituitary adenomas. The data obtained was assessed for demographic and clinical characteristics, radiographic features and post-operative outcomes. Study included 10 males and 4 females. Mean age of patients was 46.43 years (range 16-70 Years). Most common presentation was diminished vision reported in 79% patients...
March 2018: Indian Journal of Otolaryngology and Head and Neck Surgery
Jiushu Xie, Him Cheung, Manqiong Shen, Ruiming Wang
This study examines the spontaneous use of embodied egocentric transformation (EET) in understanding false beliefs in the minds of others. EET involves the participants mentally transforming or rotating themselves into the orientation of an agent when trying to adopt his or her visuospatial perspective. We argue that psychological perspective taking such as false belief reasoning may also involve EET because of what has been widely reported in the embodied cognition literature, showing that our processing of abstract, propositional information is often grounded in concrete bodily sensations which are not apparently linked to higher cognition...
February 17, 2018: Cognitive Science
Mengwen Qi, Chunfeng Wu, Zhouqing Wang, Li Zhou, Chen Men, Yimei Du, Songming Huang, Lei Chen, Ling Chen
BACKGROUND/AIMS: Glycine plays an important role in regulating hippocampal inhibitory/ excitatory neurotransmission through activating glycine receptors (GlyRs) and acting as a co-agonist of N-methyl-d-aspartate-type glutamate receptors. Activation of transient receptor potential vanilloid 4 (TRPV4) is reported to inhibit hippocampal A-type γ-aminobutyric acid receptor, a ligand-gated chloride ion channel. GlyRs are also ligand-gated chloride ion channels and this paper aimed to explore whether activation of TRPV4 could modulate GlyRs...
February 7, 2018: Cellular Physiology and Biochemistry
Mikie Hinata, Sunao Imai, Takao Sanaki, Junji Tsuchida, Takeshi Yoshioka, Kenichi Higashino, Miyuki Yamamoto, Masayuki Imai, Masahiko Soga, Narumi Horita, Isao Fukuda, Minoru Ikeda, Shoji Yamane, Atsushi Morita, Toshiyuki Kanemasa, Gaku Sakaguchi, Minoru Hasegawa, Masabumi Minami, Yasuhide Morioka
Transient receptor potential vanilloid 4 (TRPV4) receptor modulates pain, and this has been noted in several animal models. However, the involvement of TRPV4 in osteoarthritic (OA) pain remains poorly understood. The present study assessed the functional changes in TRPV4 and the expression of its endogenous ligand 5,6-epoxyeicosatrienoic acid (5,6-EET) in a rat monoiodoacetate (MIA)-induced OA pain model (MIA rats). MIA-treated rats showed reduced grip strength as compared with sham-treated rats, and this loss in function could be recovered by the intra-articular administration of a TRPV4 antagonist (HC067047 or GSK2193874)...
February 9, 2018: Pain
Tiago Felipe Senes-Lopes, Jorge Alberto López, Viviane Souza do Amaral, José Brandão-Neto, Adriana Augusto de Rezende, Jefferson Romáryo Duarte da Luz, Zaira da Rosa Guterres, Maria das Graças Almeida
Medicinal plants have been used in primary healthcare since the earliest days of humankind. Turnera subulata and Spondias mombin × Spondias tuberosa are widely used in the Brazilian Northeast to treat several diseases. The aim of this study was to evaluate the genotoxic effects of the leaf extracts of these species by the somatic mutation and recombination test in the somatic cells of Drosophila melanogaster wings. The experiments were performed using standard and high-bioactivation cross and three concentrations of the test substance [aqueous extract (AET and AES) at 5...
February 13, 2018: Journal of Medicinal Food
Jian Lu, Daozhi Liu, Xiaojing Zhou, Ang Chen, Zhenran Jiang, Xiyun Ye, Mingyao Liu, Xin Wang
BACKGROUND: Cytochrome P450 2J2 (CYP2J2) is not only highly expressed in many kinds of human tumors, but also promotes tumor cell growth via regulating the metabolism of arachidonic acids. CYP2J2 inhibitors can significantly reduce proliferation, migration and promote apoptosis of tumor cells by inhibiting epoxyeicosatrienoic acids (EETs) biosynthesis. Therefore screening CYP2J2 inhibitors is a significant way for the development of anti-cancer drug. PURPOSE: The aim of this study was to identify a new CYP2J2 inhibitor from fifty natural compounds obtained from plants...
January 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
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