keyword
MENU ▼
Read by QxMD icon Read
search

assembly

keyword
https://www.readbyqxmd.com/read/28346895/wheat-genomics-comes-of-age
#1
REVIEW
Cristobal Uauy
Advances in wheat genomics have lagged behind other major cereals (e.g., rice and maize) due to its highly repetitive and large polyploid genome. Recent technological developments in sequencing and assembly methods, however, have largely overcome these barriers. The community now moves to an era centred on functional characterisation of the genome. This includes understanding sequence and structural variation as well as how information is integrated across multiple homoeologous genomes. This understanding promises to uncover variation previously hidden from natural and human selection due to the often observed functional redundancy between homoeologs...
March 24, 2017: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/28346861/biomimetic-remineralization-of-carious-lesions-by-self-assembling-peptide
#2
L Kind, S Stevanovic, S Wuttig, S Wimberger, J Hofer, B Müller, U Pieles
Caries is the most common disease in the world. Great efforts have been undertaken for prevention and to identify a regenerative treatment solution for dental caries. Self-assembling β-sheet forming peptides have previously shown to form 3-dimensional fiber networks supporting tissue regeneration. In particular, the self-assembling peptide P11-4 has shown potential in the treatment and prevention of dental caries. It has previously been shown that application of monomeric P11-4 solution to early carious lesions can increase net mineral gain by forming de novo hydroxyapatite crystals...
March 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28346787/assembly-and-speed-in-ion-exchange-based-modular-phoretic-micro-swimmers
#3
Ran Niu, Denis Botin, Julian Weber, Alexander Reinmüller, Thomas Palberg
We report an experimental study on ion-exchange based modular micro-swimmers in low-salt water. Cationic ion-exchange particles and passive cargo particles assemble into self-propelling complexes, showing self-propulsion at speeds of several microns per second over extended distances and times. We quantify the assembly and speed of the complexes for different combinations of ion exchange particles and cargo particles, substrate types, salt types and concentrations, and cell geometries. Irrespective of experimental boundary conditions, we observe a regular development of the assembly shape with increasing number of cargo...
March 27, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28346778/direct-measurement-of-the-angular-pair-correlations-coefficients-in-molecular-liquids-using-nmr-benchmarking-force-fields-for-atomistic-simulations
#4
Leah Marie Heist, Chi-Duen Poon, Edward Thaddeus Samulski, Demetri J Photinos
High field deuterium NMR spectroscopy is used to characterize a number of molecular liquids and their mixtures in order to probe the directional part of the intermolecular interactions through the orientational ordering induced in the isotropic liquid phase by the spectrometer magnetic field. The systems studied include benzene, chloroform, hexafluorobenzene and thiophene at various concentrations and in mixtures. Dilution with the magnetically isotropic tetramethylsilane provides quantification of ordering at "infinite magnetic dilution", i...
March 27, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28346751/d-fructose-modification-enhanced-internalization-of-mixed-micelles-in-breast-cancer-cells-via-glut5-transporters
#5
Xu Zhou, Xianyan Qin, Tao Gong, Zhi-Rong Zhang, Yao Fu
d-Fructose modified poly(ε-caprolactone)-polyethylene glycol (PCL-PEG-Fru) diblock amphiphile is synthesized via Cu(I)-catalyzed click chemistry, which self-assembles with D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) into PCL-PEG-Fru/TPGS mixed micelles (PPF MM). It has been proven that glucose transporter (GLUT)5 is overexpressed in MCF-7 cells other than L929 cells. In this study, PPF MM exhibit a significantly higher uptake efficiency than fructose-free PCL-PEG-N3 /TPGS mixed micelles in both 2D MCF-7 cells and 3D tumor spheroids...
March 27, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28346728/nubpl-a-novel-metastasis-related-gene-promotes-colorectal-carcinoma-cell-motility-by-inducing-emt
#6
Yuhui Wang, Nan Wu, Donglin Sun, Haiming Sun, Dandan Tong, Duo Liu, Bo Pang, Su Li, Jia Wei, Jialin Dai, Yang Liu, Jing Bai, Jingshu Geng, Songbin Fu, Yan Jin
NUBPL is an assembly factor for human mitochondrial complex I, which is the biggest member of mitochondrial respiratory chain. However, the relationship between NUBPL and carcinoma progression remains unknown. In this study, NUBPL was characterized for its role in colorectal cancer (CRC) and the underlying molecular mechanisms. Data (n=197) from Oncomine database revealed that mRNA levels of NUBPL were remarkably overexpressed in CRC tissues compared with normal tissues. In addition, immunohistochemical analysis of 75 pairs of CRC and nontumorous tissues demonstrated that expression level of NUBPL was significantly higher in CRC tissues, and its expression level was positively associated with lymph node metastasis (P=0...
March 27, 2017: Cancer Science
https://www.readbyqxmd.com/read/28346558/simple-synthesis-of-multiple-length-scale-structured-nb2o5-with-functional-macrodomain-integrated-mesoporous-frameworks
#7
Seongseop Kim, Inyoung Jeong, Jongkook Hwang, Min Jae Ko, Jinwoo Lee
We report the simple synthesis of macro- and mesostructured Nb2O5 that have functional submicrometer-sized particles (macrodomain) embedded in mesoporous frameworks (nanodomain). Resol can macrophase-separate by self-polymerization and co-assemble with niobia sol into mesostructured frameworks. The resultant materials increase the power conversion efficiency due to light-scattering capability of submicrometer-sized particles.
March 27, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28346555/modulation-of-the-cooperativity-in-the-assembly-of-multistranded-supramolecular-polymers
#8
Cristiana Campanella, Elkin Lopez-Fontal, Lilia Milanesi, Salvador Tomas
It is highly desirable that supramolecular polymers self-assemble following small changes in the environment. The degree of responsiveness depends on the degree of cooperativity at play during the assembly. Understanding how to modulate and quantify cooperativity is therefore highly desirable for the study and design of responsive polymers. Here we show that the cooperative assembly of a porphyrin-based, double-stranded polymer is triggered by changes in building blocks and in salt concentration. We develop a model that accounts for this responsiveness by assuming the binding of the salt countercations to the double-stranded polymer...
March 27, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28346465/sept12-phosphorylation-results-in-loss-of-the-septin-ring-sperm-annulus-defective-sperm-motility-and-poor-male-fertility
#9
Yi-Ru Shen, Han-Yu Wang, Yung-Che Kuo, Shih-Chuan Shih, Chun-Hua Hsu, Yet-Ran Chen, Shang-Rung Wu, Chia-Yih Wang, Pao-Lin Kuo
Septins are critical for numerous cellular processes through the formation of heteromeric filaments and rings indicating the importance of structural regulators in septin assembly. Several posttranslational modifications (PTMs) mediate the dynamics of septin filaments in yeast. However, little is known about the role of PTMs in regulating mammalian septin assembly, and the in vivo significance of PTMs on mammalian septin assembly and function remains unknown. Here, we showed that SEPT12 was phosphorylated on Ser198 using mass spectrometry, and we generated SEPT12 phosphomimetic knock-in (KI) mice to study its biological significance...
March 27, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28346459/directed-self-assembly-a-dress-code-for-block-copolymers
#10
Christopher K Ober
No abstract text is available yet for this article.
March 27, 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28346456/sub-10-nm-patterning-via-directed-self-assembly-of-block-copolymer-films-with-a-vapour-phase-deposited-topcoat
#11
Hyo Seon Suh, Do Han Kim, Priya Moni, Shisheng Xiong, Leonidas E Ocola, Nestor J Zaluzec, Karen K Gleason, Paul F Nealey
Directed self-assembly (DSA) of the domain structure in block copolymer (BCP) thin films is a promising approach for sub-10-nm surface patterning. DSA requires the control of interfacial properties on both interfaces of a BCP film to induce the formation of domains that traverse the entire film with a perpendicular orientation. Here we show a methodology to control the interfacial properties of BCP films that uses a polymer topcoat deposited by initiated chemical vapour deposition (iCVD). The iCVD topcoat forms a crosslinked network that grafts to and immobilizes BCP chains to create an interface that is equally attractive to both blocks of the underlying copolymer...
March 27, 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28346455/collective-helicity-switching-of-a-dna-coat-assembly
#12
Yongju Kim, Huichang Li, Ying He, Xi Chen, Xiaoteng Ma, Myongsoo Lee
Hierarchical assemblies of biomolecular subunits can carry out versatile tasks at the cellular level with remarkable spatial and temporal precision. As an example, the collective motion and mutual cooperation between complex protein machines mediate essential functions for life, such as replication, synthesis, degradation, repair and transport. Nucleic acid molecules are far less dynamic than proteins and need to bind to specific proteins to form hierarchical structures. The simplest example of these nucleic acid-based structures is provided by a rod-shaped tobacco mosaic virus, which consists of genetic material surrounded by coat proteins...
March 27, 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28346433/a-cytosolic-ezh1-isoform-modulates-a-prc2-ezh1-epigenetic-adaptive-response-in-postmitotic-cells
#13
Beatrice Bodega, Federica Marasca, Valeria Ranzani, Alessandro Cherubini, Francesco Della Valle, Maria Victoria Neguembor, Michel Wassef, Alessio Zippo, Chiara Lanzuolo, Massimiliano Pagani, Valerio Orlando
The evolution of chromatin-based epigenetic cell memory may be driven not only by the necessity for cells to stably maintain transcription programs, but also by the need to recognize signals and allow plastic responses to environmental stimuli. The mechanistic role of the epigenome in adult postmitotic tissues, however, remains largely unknown. In vertebrates, two variants of the Polycomb repressive complex (PRC2-Ezh2 and PRC2-Ezh1) control gene silencing via methylation of histone H3 on Lys27 (H3K27me). Here we describe a reversible mechanism that involves a novel isoform of Ezh1 (Ezh1β)...
March 27, 2017: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/28346405/structural-and-functional-insight-into-human-o-glcnacase
#14
Christian Roth, Sherry Chan, Wendy A Offen, Glyn R Hemsworth, Lianne I Willems, Dustin T King, Vimal Varghese, Robert Britton, David J Vocadlo, Gideon J Davies
O-GlcNAc hydrolase (OGA) removes O-linked N-acetylglucosamine (O-GlcNAc) from a myriad of nucleocytoplasmic proteins. Through co-expression and assembly of OGA fragments, we determined the three-dimensional structure of human OGA, revealing an unusual helix-exchanged dimer that lays a structural foundation for an improved understanding of substrate recognition and regulation of OGA. Structures of OGA in complex with a series of inhibitors define a precise blueprint for the design of inhibitors that have clinical value...
March 27, 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28346404/chemical-screening-identifies-atm-as-a-target-for-alleviating-senescence
#15
Hyun Tae Kang, Joon Tae Park, Kobong Choi, Yongsub Kim, Hyo Jei Claudia Choi, Chul Won Jung, Young-Sam Lee, Sang Chul Park
Senescence, defined as irreversible cell-cycle arrest, is the main driving force of aging and age-related diseases. Here, we performed high-throughput screening to identify compounds that alleviate senescence and identified the ataxia telangiectasia mutated (ATM) inhibitor KU-60019 as an effective agent. To elucidate the mechanism underlying ATM's role in senescence, we performed a yeast two-hybrid screen and found that ATM interacted with the vacuolar ATPase V1 subunits ATP6V1E1 and ATP6V1G1. Specifically, ATM decreased E-G dimerization through direct phosphorylation of ATP6V1G1...
March 27, 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28346370/de-novo-assembly-annotation-and-characterization-of-root-transcriptomes-of-three-caladium-cultivars-with-a-focus-on-necrotrophic-pathogen-resistance-defense-related-genes
#16
Zhe Cao, Zhanao Deng
Roots are vital to plant survival and crop yield, yet few efforts have been made to characterize the expressed genes in the roots of non-model plants (root transcriptomes). This study was conducted to sequence, assemble, annotate, and characterize the root transcriptomes of three caladium cultivars (Caladium × hortulanum) using RNA-Seq. The caladium cultivars used in this study have different levels of resistance to Pythiummyriotylum, the most damaging necrotrophic pathogen to caladium roots. Forty-six to 61 million clean reads were obtained for each caladium root transcriptome...
March 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28346356/microinjection-of-antibodies-targeting-the-lamin-a-c-histone-binding-site-blocks-mitotic-entry-and-reveals-separate-chromatin-interactions-with-hp1-cenpb-and-pml
#17
Charles R Dixon, Melpomeni Platani, Alexandr A Makarov, Eric C Schirmer
Lamins form a scaffold lining the nucleus that binds chromatin and contributes to spatial genome organization; however, due to the many other functions of lamins, studies knocking out or altering the lamin polymer cannot clearly distinguish between direct and indirect effects. To overcome this obstacle, we specifically targeted the mapped histone-binding site of A/C lamins by microinjecting antibodies specific to this region predicting that this would make the genome more mobile. No increase in chromatin mobility was observed; however, interestingly, injected cells failed to go through mitosis, while control antibody-injected cells did...
March 25, 2017: Cells
https://www.readbyqxmd.com/read/28346244/a-six-sigma-approach-to-analyze-time-to-assembly-variance-of-surgical-trays-in-a-sterile-services-department
#18
Flavio S Fogliatto, Michel J Anzanello, Guilherme L Tortorella, Daniela S S Schneider, Cintia G R Pereira, Beatriz D Schaan
INTRODUCTION: We analyze the assembly of surgical trays in a hospital's sterile services department. The department assembles 520 different tray setups. However, tray assembly times are unknown, imposing a challenge to production planners. To respond to demand, workers from other departments are often called, leading to higher operational costs and more frequent quality problems due to workers' poor training and inconsistency. METHODS: Conducting traditional time-motion studies is infeasible in such a high variety production setting...
March 24, 2017: Journal for Healthcare Quality: Official Publication of the National Association for Healthcare Quality
https://www.readbyqxmd.com/read/28346153/national-sleep-foundation-s-sleep-quality-recommendations-first-report
#19
Maurice Ohayon, Emerson M Wickwire, Max Hirshkowitz, Steven M Albert, Alon Avidan, Frank J Daly, Yves Dauvilliers, Raffaele Ferri, Constance Fung, David Gozal, Nancy Hazen, Andrew Krystal, Kenneth Lichstein, Monica Mallampalli, Giuseppe Plazzi, Robert Rawding, Frank A Scheer, Virend Somers, Michael V Vitiello
OBJECTIVES: To provide evidence-based recommendations and guidance to the public regarding indicators of good sleep quality across the life-span. METHODS: The National Sleep Foundation assembled a panel of experts from the sleep community and representatives appointed by stakeholder organizations (Sleep Quality Consensus Panel). A systematic literature review identified 277 studies meeting inclusion criteria. Abstracts and full-text articles were provided to the panelists for review and discussion...
February 2017: Sleep Health
https://www.readbyqxmd.com/read/28345938/mimicking-ph-gated-ionic-channel-by-polyelectrolyte-complex-confinement-inside-single-nanopore
#20
Yixuan Zhao, Jean-Marc Janot, Emmanuel Balanzat, Sebastien Balme
Biological channels have served as inspiration to design stimuli-response artificial nanopore. Here we propose an original approach to design pH-gate nanopore based on polyethylenimine and chondroitin-4-sulfate (ChS) layer-by-layer self-assembly. This approach is interesting because it is rapid and permits a monitoring in real time of functionalization. The study of ionic transport through these single nanopores reveals a selectivity on anions and pH-gate properties at low salt concentration. It is open at pH below 4 or 5 depending salt concentration...
March 27, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
keyword
keyword
29687
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"