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Xilang Jin, Shaoping Wu, Mengyao She, Yifan Jia, Likai Hao, Bing Yin, Lanying Wang, Martin Obst, Yehua Shen, Yongmin Zhang, Jianli Li
A broad-spectrum fluorescent probe, which can be applied to monitoring H2S in various biological systems, has been synthesized. This specific probe was applied to localize the endogenous H2S in living Raw264.7 macrophages cells, HepG2 cells and H9C2 cells. The probe have successfully visualized CBS- and CSE-induced endogenous H2S production and monitored CBS and CSE activity in H9C2 cells. This probe could serve as a powerful molecular imaging tool to further explore the physiological function and the molecular mechanisms of endogenous H2S in living animal systems...
October 26, 2016: Analytical Chemistry
Alex R Hardisty, Finn Bacall, Niall Beard, Maria-Paula Balcázar-Vargas, Bachir Balech, Zoltán Barcza, Sarah J Bourlat, Renato De Giovanni, Yde de Jong, Francesca De Leo, Laura Dobor, Giacinto Donvito, Donal Fellows, Antonio Fernandez Guerra, Nuno Ferreira, Yuliya Fetyukova, Bruno Fosso, Jonathan Giddy, Carole Goble, Anton Güntsch, Robert Haines, Vera Hernández Ernst, Hannes Hettling, Dóra Hidy, Ferenc Horváth, Dóra Ittzés, Péter Ittzés, Andrew Jones, Renzo Kottmann, Robert Kulawik, Sonja Leidenberger, Päivi Lyytikäinen-Saarenmaa, Cherian Mathew, Norman Morrison, Aleksandra Nenadic, Abraham Nieva de la Hidalga, Matthias Obst, Gerard Oostermeijer, Elisabeth Paymal, Graziano Pesole, Salvatore Pinto, Axel Poigné, Francisco Quevedo Fernandez, Monica Santamaria, Hannu Saarenmaa, Gergely Sipos, Karl-Heinz Sylla, Marko Tähtinen, Saverio Vicario, Rutger Aldo Vos, Alan R Williams, Pelin Yilmaz
BACKGROUND: Making forecasts about biodiversity and giving support to policy relies increasingly on large collections of data held electronically, and on substantial computational capability and capacity to analyse, model, simulate and predict using such data. However, the physically distributed nature of data resources and of expertise in advanced analytical tools creates many challenges for the modern scientist. Across the wider biological sciences, presenting such capabilities on the Internet (as "Web services") and using scientific workflow systems to compose them for particular tasks is a practical way to carry out robust "in silico" science...
October 20, 2016: BMC Ecology
Ademar Caldeira, William Correia Trinca, Thais Pires Flores, Andrea Barleze Costa, Claudio de Sá Brito, Karen Loureiro Weigert, Maryana Schwartzhaupt Matos, Carmela Nicolini, Fernando Mariano Obst
Kindler syndrome1, 2 is a genetic disorder mainly characterized by increased skin fragility and photosensitivity,3, 4 making the use of treatments based on radiation difficult or even prohibited. Thus, cases reporting Kindler syndrome patients treated with radiotherapy are rare. In this study, we report clinical outcomes and care provided for a rare case of a Kindler syndrome patient submitted to radiotherapy. Diagnosed with squamous cell carcinoma involving the buccal mucosa, the patient was exclusively treated with radiotherapy, with 70 Gy delivered on the PTV with the Volumetric Modulated Arc technique...
November 2016: Reports of Practical Oncology and Radiotherapy
Holger Kühne, Ulrike Obst-Sander, Bernd Kuhn, Aurelia Conte, Simona M Ceccarelli, Werner Neidhart, Markus G Rudolph, Giorgio Ottaviani, Rodolfo Gasser, Sung-Sau So, Shirley Li, Xiaolei Zhang, Lin Gao, Michael Myers
Dual inhibition of fatty acid binding proteins 4 and 5 (FABP4 and FABP5) is expected to provide beneficial effects on a number of metabolic parameters such as insulin sensitivity and blood glucose levels and should protect against atherosclerosis. Starting from a FABP4 selective focused screening hit, biostructure information was used to modulate the selectivity profile in the desired way and to design potent dual FABP4/5 inhibitors with good selectivity against FABP3. With very good pharmacokinetic properties and no major safety alerts, compound 12 was identified as a suitable tool compound for further in vivo investigations...
October 15, 2016: Bioorganic & Medicinal Chemistry Letters
J Metzkes, K Zeil, S D Kraft, L Karsch, M Sobiella, M Rehwald, L Obst, H-P Schlenvoigt, U Schramm
In this paper, a scintillator-based online beam profile detector for the characterization of laser-driven proton beams is presented. Using a pixelated matrix with varying absorber thicknesses, the proton beam is spatially resolved in two dimensions and simultaneously energy-resolved. A thin plastic scintillator placed behind the absorber and read out by a CCD camera is used as the active detector material. The spatial detector resolution reaches down to ∼4 mm and the detector can resolve proton beam profiles for up to 9 proton threshold energies...
August 2016: Review of Scientific Instruments
Sarah Houben-Wilke, Rudolf A Jörres, Robert Bals, Frits M E Franssen, Sven Gläser, Rolf Holle, Annika Karch, Armin Koch, Helgo Magnussen, Anne Obst, Holger Schulz, Martijn A Spruit, Margarethe E Wacker, Tobias Welte, Emiel F M Wouters, Claus Vogelmeier, Henrik Watz
RATIONALE: Knowledge about the prevalence of objectively assessed peripheral artery disease (PAD) and its clinical relevance in patients with COPD is scarce. OBJECTIVES: We aimed (1) to assess the prevalence of PAD in COPD compared to distinct control groups and (2) to study the association between PAD and functional capacity as well as health status. METHODS: The ankle-brachial index (ABI) was used to diagnose PAD (ABI≤0.9). 6-Minute-Walk-Distance (6MWD), health status (St...
August 17, 2016: American Journal of Respiratory and Critical Care Medicine
Jim Oxtoby, Robert King, Judith Sheridan, Patricia Obst
The Multidimensional State Boredom Scale (MSBS) is a promising new self-report measure of state boredom. Two condensed versions of the scale have also been introduced. This study helped explore the psychometric qualities of these scales, using a large sample of Australian adults (N = 1,716), as well as two smaller samples (N = 199 and N = 422). Data analyses indicated strong convergent validity and very high internal consistency for the scales. Test-retest reliability over a 6- to 8-day period was moderately high...
August 9, 2016: Assessment
Mengyao She, Zheng Yang, Likai Hao, Zhaohui Wang, Tianyou Luo, Martin Obst, Ping Liu, Yehua Shen, Shengyong Zhang, Jianli Li
A series of Cu(2+) probe which contains 9 probes have been synthesized and established. All the probes were synthesized using Rhodamine B as the fluorophore, conjugated to various differently substituted cinnamyl aldehyde with C=N Schiff base structural motif as their core moiety. The structure-property relationships of these probes have been investigated. The change of optical properties, caused by different electronic effect and steric effect of the recognition group, has been analyzed systematically. DFT calculation simulation of the Ring-Close and Ring-Open form of all the probes have been employed to illuminate, summarize and confirm these correlations between optical properties and molecular structures...
2016: Scientific Reports
L Nuri, S J Obst, R Newsham-West, R S Barrett
Our understanding of in vivo Achilles tendon (AT) conditioning is limited to two-dimensional ultrasound measures of longitudinal deformation of the whole tendon. This study investigated the regional three-dimensional (3D) deformation of the AT during conditioning. Eighteen ATs were scanned using 3D freehand ultrasound during 10 successive 25 s submaximal (50%) voluntary isometric plantarflexion contractions. Longitudinal strain was assessed for the whole AT, aponeurosis, and free AT and transverse strain was assessed for the proximal-, mid-, and distal-portions of the free AT...
August 3, 2016: Scandinavian Journal of Medicine & Science in Sports
D Lee, R Barrett, M Ryan, D J Saxby, R Newsham-West, S J Obst
This study investigated strain differences within the patellar tendon (PT) mid-region using an ultrasound-based digital image correlation (DIC) method. Six healthy young participants performed six knee extensions to 60% of maximal voluntary isometric contraction on 2 days. Sagittal ultrasound videos recorded during each contraction were analyzed using the DIC method to determine the strain-torque relationships of the superficial, deep, and whole PT mid-regions. Significantly greater strain was observed in the deep vs superficial layer of the PT mid-region for all contraction intensities, with peak strains of 5...
August 3, 2016: Scandinavian Journal of Medicine & Science in Sports
Lars Schreiber, Kasper U Kjeldsen, Peter Funch, Jeppe Jensen, Matthias Obst, Susanna López-Legentil, Andreas Schramm
Ascidians are marine filter feeders and harbor diverse microbiota that can exhibit a high degree of host-specificity. Pharyngeal samples of Scandinavian and Mediterranean ascidians were screened for consistently associated bacteria by culture-dependent and -independent approaches. Representatives of the Endozoicomonas (Gammaproteobacteria, Hahellaceae) clade were detected in the ascidian species Ascidiella aspersa, Ascidiella scabra, Botryllus schlosseri, Ciona intestinalis, Styela clava, and multiple Ascidia/Ascidiella spp...
2016: Frontiers in Microbiology
Helen M Haydon, Patricia L Obst, Ioni Lewis
BACKGROUND: Changing trends demonstrate that women, in a number of economically-developed countries, are drinking at higher levels than ever before. Exploring key targets for intervention, this study examined the extent to which underlying beliefs in relation to alcohol consumption predicted intentions to drink in three different ways (i.e. low risk drinking, frequent drinking and binge drinking). METHODS: Utilizing a prospective design survey, women (N = 1069), aged 18-87 years, completed a questionnaire measuring their beliefs and intentions regarding alcohol consumption...
2016: BMC Women's Health
Hanna Kische, Ralf Ewert, Ingo Fietze, Stefan Gross, Henri Wallaschofski, Henry Völzke, Marcus Dörr, Matthias Nauck, Anne Obst, Beate Stubbe, Thomas Penzel, Robin Haring
CONTEXT AND OBJECTIVES: Associations between sex hormones and sleep habits originate mainly from small and selected patient-based samples. We examined data from a population-based sample with various sleep characteristics and the major part of sex hormones measured by mass spectrometry. DESIGN, SETTING AND PARTICIPANTS: We used data from 204 men and 213 women of the cross-sectional Study of Health in Pomerania-TREND. MAIN OUTCOME AND MEASURES: Associations of total and free testosterone (TT, fT), androstenedione (ASD), estrone (E1), estradiol (E2), dehydroepiandrosterone-sulphate (DHEAS), sex hormone-binding globulin (SHBG), and E2/TT ratio with sleep measures (including total sleep time, sleep efficiency, wake after sleep onset (WASO), apnea-hypopnea index (AHI), Insomnia Severity Index, Epworth Sleepiness Scale (ESS), and Pittsburgh Sleep Quality Index) were assessed by sex-specific multivariable regression models...
July 12, 2016: Journal of Clinical Endocrinology and Metabolism
Lars Schreiber, Kasper Urup Kjeldsen, Matthias Obst, Peter Funch, Andreas Schramm
Two gram-negative, facultative anaerobic, chemoorganoheterotrophic, motile and rod-shaped bacteria, strains AVMART05(T) and KASP37, were isolated from ascidians (Tunicata, Ascidiaceae) of the genus Ascidiella collected at Gullmarsfjord, Sweden. The strains are the first cultured representatives of an ascidian-specific lineage within the genus Endozoicomonas (Gammaproteobacteria, Oceanospirillales, Hahellaceae). Both strains feature three distinct 16S rRNA gene paralogs, with identities of 98.9-99.1% (AVMART05(T)) and 97...
July 2016: Systematic and Applied Microbiology
Jessica Walker, Katharina V Reichelt, Katja Obst, Sabine Widder, Joachim Hans, Gerhard E Krammer, Jakob P Ley, Veronika Somoza
Polyphenol-rich plant extracts have been shown to possess anti-inflammatory activity against oral pathogen-induced cytokine release in model systems of inflammation. Here, it was hypothesized that a flavanone-rich extract of E. angustifolium exhibits an anti-inflammatory potential against endotoxin-induced inflammatory response in human gingival fibroblasts (HGF-1). HGF-1 cells were stimulated with lipopolysaccharide from Porphyromonas gingivalis (pg-LPS) to release pro-inflammatory cytokines. Concentrations of interleukins IL-6 and IL-8 and macrophage chemoattractant protein-1 in the incubation media upon stimulation were determined by means of magnetic bead analysis...
July 13, 2016: Food & Function
Michele Pinelli, Annamaria Carissimo, Luisa Cutillo, Ching-Hung Lai, Margherita Mutarelli, Maria Nicoletta Moretti, Marwah Veer Singh, Marianthi Karali, Diego Carrella, Mariateresa Pizzo, Francesco Russo, Stefano Ferrari, Diego Ponzin, Claudia Angelini, Sandro Banfi, Diego di Bernardo
The human retina is a specialized tissue involved in light stimulus transduction. Despite its unique biology, an accurate reference transcriptome is still missing. Here, we performed gene expression analysis (RNA-seq) of 50 retinal samples from non-visually impaired post-mortem donors. We identified novel transcripts with high confidence (Observed Transcriptome (ObsT)) and quantified the expression level of known transcripts (Reference Transcriptome (RefT)). The ObsT included 77 623 transcripts (23 960 genes) covering 137 Mb (35 Mb new transcribed genome)...
July 8, 2016: Nucleic Acids Research
Oliver Baker, Ashish Gupta, Mandy Obst, Youming Zhang, Konstantinos Anastassiadis, Jun Fu, A Francis Stewart
A fluent method for gene targeting to establish protein tagged and ligand inducible conditional loss-of-function alleles is described. We couple new recombineering applications for one-step cloning of gRNA oligonucleotides and rapid generation of short-arm (~1 kb) targeting constructs with the power of Cas9-assisted targeting to establish protein tagged alleles in embryonic stem cells at high efficiency. RAC (Recombineering And Cas9)-tagging with Venus, BirM, APEX2 and the auxin degron is facilitated by a recombineering-ready plasmid series that permits the reuse of gene-specific reagents to insert different tags...
2016: Scientific Reports
Brian D Stadinski, Reinhard Obst, Eric S Huseby
The ability of a single T cell antigen receptor (TCR) to cross-react with multiple antigens allows the finite number of T cells within an organism to respond to the compendium of pathogen challenges faced during a lifetime. Effective immune surveillance, however, comes at a price. TCR cross-reactivity can allow molecular mimics to spuriously activate autoimmune T cells; it also underlies T cell rejection of organ transplants and drives graft-versus-host disease. In this issue of the JCI, Cole and colleagues provide insight into how an insulin-reactive T cell cross-reacts with pathogen-derived antigens by focusing on a limited portion of the peptides to provide a hotspot for binding...
June 1, 2016: Journal of Clinical Investigation
Thomas Kötter, Yannick Tautphäus, Katrin U Obst, Edgar Voltmer, Martin Scherer
CONTEXT: The freshman year of medical school is a stressful period in the lives of future doctors. Resilience to this stress differs greatly, leading to different health outcomes. Less resilient students, whose health may deteriorate early in their education, are at greater risk for developing stress-related diseases. Early identification of individuals at risk and the provision of tailored health-promoting interventions might prevent this. OBJECTIVES: This study was designed to investigate: (i) how the health of medical students develops over the freshman year, and (ii) whether certain protective factors can predict general and mental health status after 1 year of medical education...
June 2016: Medical Education
Stacey A Mackay, Katherine M White, Patricia L Obst
Microvolunteering is bite-size volunteering with no commitment to repeat and minimum formality, involving short and specific actions. Online microvolunteering occurs through an internet-connected device. University students' online microvolunteering decisions were investigated using an extended theory of planned behavior (TPB) comprising attitudes and normative and control perceptions, with the additional variables of moral norm and group norm. Participants (N = 303) completed the main TPB questionnaire and 1-month follow-up survey (N = 171) assessing engagement in online microvolunteering...
April 2016: Cyberpsychology, Behavior and Social Networking
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