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https://www.readbyqxmd.com/read/29196683/preferential-selection-of-arginine-at-the-lipid-water-interface-of-trpv1-during-vertebrate-evolution-correlates-with-its-snorkeling-behaviour-and-cholesterol-interaction
#1
Somdatta Saha, Arijit Ghosh, Nikhil Tiwari, Ashutosh Kumar, Abhishek Kumar, Chandan Goswami
TRPV1 is a thermo-sensitive ion channel involved in neurosensory and other physiological functions. The trans-membrane helices of TRPV1 undergo quick and complex conformational changes governed by thermodynamic parameters and membrane components leading to channel opening. However, the molecular mechanisms underlying such events are poorly understood. Here we analysed the molecular evolution of TRPV1 at the lipid-water-interface region (LWI), typically defined as a layer of 6 Å thickness on each side of the membrane with less availability of free water...
December 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29186227/-snorkelling-vs-diving-in-mixed-micelles-probed-by-means-of-a-molecular-bathymeter
#2
Gemma M Rodriguez-Muñiz, Miguel Gomez-Mendoza, Edurne Nuin, Inmaculada Andreu, M Luisa Marin, Miguel A Miranda
A photoactive bathymeter based on a carboxylic acid moiety covalently linked to a signalling methoxynaphthalene (MNP) fluorophore has been designed to prove the concept of "snorkelling" vs. "diving" in mixed micelles (MM). The carboxylic acid "floats" on the MM surface, while the MNP unit sinks deep in MM. The rate constants of MNP fluorescence quenching by iodide, which remains basically in water, consistently decrease with increasing spacer length, revealing different regions. This is associated with the distance MNP should "dive" in MM to achieve protection from aqueous reactants...
November 29, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29153534/configuration-affects-parallel-stent-grafting-results
#3
Adam Tanious, Mathew Wooster, Paul A Armstrong, Bruce Zwiebel, Shane Grundy, Martin R Back, Murray L Shames
OBJECTIVE: A number of adjunctive "off-the-shelf" procedures have been described to treat complex aortic diseases. Our goal was to evaluate parallel stent graft configurations and to determine an optimal formula for these procedures. METHODS: This is a retrospective review of all patients at a single medical center treated with parallel stent grafts from January 2010 to September 2015. Outcomes were evaluated on the basis of parallel graft orientation, type, and main body device...
November 16, 2017: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/29125878/insight-into-the-interactions-residue-snorkeling-and-membrane-disordering-potency-of-a-single-antimicrobial-peptide-into-different-lipid-bilayers
#4
Majid Jafari, Faramarz Mehrnejad, Farahnoosh Doustdar
Pardaxin, with a bend-helix-bend-helix structure, is a membrane-active antimicrobial peptide that its membrane activity depends on the lipid bilayer composition. Herein, all-atom molecular dynamics (MD) simulations were performed to provide further molecular insight into the interactions, structural dynamics, orientation behavior, and cationic residues snorkeling of pardaxin in the DMPC, DPPC, POPC, POPG, POPG/POPE (3:1), and POPG/POPE (1:3) lipid bilayers. The results showed that the C-terminal helix of the peptide was maintained in all six types of the model-bilayers and pardaxin was tilted into the DMPC, DPPC, and POPG/POPE mixed bilayers more than the POPC and POPG bilayers...
2017: PloS One
https://www.readbyqxmd.com/read/29096975/plastic-debris-collars-an-underreported-stressor-in-tropical-reef-fishes
#5
Joséde Anchieta C C Nunes, Cláudio L S Sampaio, Francisco Barros, Antoine O H C Leduc
Plastic debris collar wrappings (PDCW) are involved in the frequent entanglement of several groups of marine animals. In fishes, however aside from 'ghost fishing', PDCW events are rarely documented, and no record of this occurrence exists in tropical reef fishes. Here, we present records for four species afflicted by plastic debris collars. Observations occurred during snorkeling, and included the silver mojarra Eucinostomus argenteus, Atlantic thread herring Ophistonema oglinum, tomtate grunt Haemulon aurolineatum and gray parrotfish Sparisoma axillare...
October 30, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/29020640/bridging-spatially-segregated-redox-zones-with-a-microbial-electrochemical-snorkel-triggers-biogeochemical-cycles-in-oil-contaminated-river-tyne-uk-sediments
#6
Carolina Cruz Viggi, Bruna Matturro, Emanuela Frascadore, Susanna Insogna, Alessio Mezzi, Saulius Kaciulis, Angela Sherry, Obioma K Mejeha, Ian M Head, Eleni Vaiopoulou, Korneel Rabaey, Simona Rossetti, Federico Aulenta
Marine sediments represent an important sink for a number of anthropogenic organic contaminants, including petroleum hydrocarbons following an accidental oil spill. Degradation of these compounds largely depends on the activity of sedimentary microbial communities linked to biogeochemical cycles, in which abundant elements such as iron and sulfur are shuttled between their oxidized and reduced forms. Here we show that introduction of a small electrically conductive graphite rod ("the electrochemical snorkel") into an oil-contaminated River Tyne (UK) sediment, so as to create an electrochemical connection between the anoxic contaminated sediment and the oxygenated overlying water, has a large impact on the rate of metabolic reactions taking place in the bulk sediment...
October 3, 2017: Water Research
https://www.readbyqxmd.com/read/28990466/task-dependent-cold-stress-during-expeditions-in-antarctic-environments
#7
Drew M Morris, June J Pilcher, Robert B Powell
This study seeks to understand the degree of body cooling, cold perception and physical discomfort during Antarctic tour excursions. Eight experienced expedition leaders across three Antarctic cruise voyages were monitored during occupational tasks: kayaking, snorkelling and zodiac outings. Subjective cold perception and discomfort were recorded using a thermal comfort assessment and skin temperature was recorded using a portable data logger. Indoor cabin temperature and outdoor temperature with wind velocity were used as measures of environmental stress...
2017: International Journal of Circumpolar Health
https://www.readbyqxmd.com/read/28918189/effects-of-temperature-and-peg-grafting-density-on-the-translocation-of-pegylated-nanoparticles-across-asymmetric-lipid-membrane
#8
Zuoheng Zhang, Xubo Lin, Ning Gu
Plasma membrane internalization of nanoparticles (NPs) is important for their biomedical applications such as drug-delivery carriers. On one hand, in order to improve their half-life in circulation, PEGylation has been widely used. However, it may hinder the NPs' membrane internalization ability. On the other hand, higher temperature could enhance the membrane permeability and may affect the NPs' ability to enter into or exit from cells. To make full use of their advantages, we systematically investigated the effects of temperature and PEG density on the translocation of PEGylated nanoparticles across the plasma asymmetric membrane of eukaryotic cells, using near-atom level coarse-grained molecular dynamics simulations...
September 6, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28873449/benthic-community-structure-on-coral-reefs-exposed-to-intensive-recreational-snorkeling
#9
Bobbie Renfro, Nanette E Chadwick
Chronic anthropogenic disturbances on coral reefs in the form of overfishing and pollution can shift benthic community composition away from stony corals and toward macroalgae. The use of reefs for recreational snorkeling and diving potentially can lead to similar ecological impacts if not well-managed, but impacts of snorkeling on benthic organisms are not well understood. We quantified variation in benthic community structure along a gradient of snorkeling frequency in an intensively-visited portion of the Mesoamerican Barrier Reef...
2017: PloS One
https://www.readbyqxmd.com/read/28774222/transradial-delivery-of-a-renal-snorkel-during-complex-endovascular-aortic-aneurysm-repair
#10
Jordan R Stern, Sharif H Ellozy
Transradial access has been described in a variety of clinical contexts but has been rarely utilized for visceral artery interventions and during complex endovascular aortic aneurysm repair (EVAR) when upper extremity access is required. This is usually accomplished via brachial artery access, and although brachial access is generally safe and effective, radial access may offer some benefits with regard to patient comfort and potential complications. Here we report a case of successful delivery of a renal snorkel via a radial artery approach during EVAR...
October 2017: Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/28739454/parallel-grafts-used-in-combination-with-physician-modified-fenestrated-stent-grafts-for-complex-aortic-aneurysms-in-high-risk-patients-with-hostile-anatomies
#11
Joseph Touma, Dorian Verscheure, Marek Majewski, Pascal Desgranges, Frederic Cochennec
OBJECTIVE: To evaluate the feasibility and early outcomes of complex aortic aneurysm repair in high risk patients with hostile anatomies using both parallel stents and physician-modified stent-graft (PMSG) techniques to address the renovisceral arteries METHODS: Consecutive patients with complex suprarenal (SRA) and thoracoabdominal aortic aneurysms (TAAA) undergoing endovascular repair using combined parallel stents technique and PMSG between September 2013 and November 2015 were evaluated...
July 21, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28700927/charged-antimicrobial-peptides-can-translocate-across-membranes-without-forming-channel-like-pores
#12
Jakob P Ulmschneider
How can highly charged, cationic antimicrobial peptides (AMPs) translocate across hydrophobic lipid bilayers despite the prohibitive energetic penalty to do so? A common explanation has been the formation of peptide-lined channels. However, for most AMPs, no structures of membrane pores have been found despite clear evidence of membrane leakage and antimicrobial activity. The study here suggests an alternative and simple reason: for the AMP PGLa from Xenopus laevis (charge +5), such pores are not needed to explain both leakage and peptide translocation...
July 11, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28689952/a-novel-off-the-shelf-technique-for-endovascular-repair-of-type-iii-and-iv-thoracoabdominal-aortic-aneurysms-using-the-gore-excluder-and-viabahn-branches
#13
Mathew Wooster, Adam Tanious, R Wesley Jones, Paul Armstrong, Murray Shames
BACKGROUND: The aim of this study is to describe the use of a novel off-the-shelf technique to repair type III and type IV thoracoabdominal aortic aneurysms (TAAAs) in the absence of available prefabricated branched devices. METHODS: All patients undergoing endovascular repair of type III and IV TAAAs using this technique were included from a prospectively maintained registry at a regional aortic referral center. The proximal bifurcated Gore C3 Excluder device is positioned in the descending thoracic aorta with the contralateral gate 2-3 cm above the celiac artery...
July 8, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28688876/concomitant-coil-embolization-for-gutter-leak-during-endovascular-aortic-repair-with-the-snorkel-technique
#14
Koji Maeda, Takao Ohki, Yuji Kanaoka, Takeshi Baba, Masahiro Tezuka, Hikaru Nakagawa
Snorkel endovascular aortic repair (SEVAR) is reported to be effective for the treatment of pararenal or juxtarenal aortic aneurysms. SEVAR can be performed with an off-the-self device, which is applied for emergency cases. However, there is a concern that SEVAR lead to gutter leak due to insertion of multiple stents. Previously, we performed 2-staged treatment for gutter leak after SEVAR. However, the gutter leaks can also occur late, and it is often difficult to close. Therefore, if a significant gutter leak is identified intraoperatively, performing concomitant gutter coil embolization at the time of the initial surgery may be reasonable...
July 6, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28611751/cable-bacteria-and-the-bioelectrochemical-snorkel-the-natural-and-engineered-facets-playing-a-role-in-hydrocarbons-degradation-in-marine-sediments
#15
Bruna Matturro, Carolina Cruz Viggi, Federico Aulenta, Simona Rossetti
The composition and metabolic traits of the microbial communities acting in an innovative bioelectrochemical system were here investigated. The system, known as Oil Spill Snorkel, was recently developed to stimulate the oxidative biodegradation of petroleum hydrocarbons in anoxic marine sediments. Next Generation Sequencing was used to describe the microbiome of the bulk sediment and of the biofilm growing attached to the surface of the electrode. The analysis revealed that sulfur cycling primarily drives the microbial metabolic activities occurring in the bioelectrochemical system...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28542802/comparison-of-nine-different-methods-to-assess-fish-communities-in-lentic-flood-plain-habitats
#16
M Mueller, J Pander, J Knott, J Geist
This study compares the effectiveness and representativeness of electrofishing, snorkelling, seining, baited lift netting, multi-mesh gillnetting, baited fish traps, fyke netting, angling and longline fishing, considering three typical lentic flood-plain habitats at different times of day. Electrofishing was by far the most effective method yielding highest species richness, species trait representation and catch per unit of effort (CPUE), followed by seining. For single species like dace Leuciscus leuciscus, European ruffe Gymnocephalus cernua, common bream Abramis brama and silver bream Blicca bjoerkna, seining was more effective than electrofishing...
May 22, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28482368/validity-of-postexercise-measurements-to-estimate-peak-vo2-in-200-m-and-400-m-maximal-swims
#17
Ferran A Rodríguez, Diego Chaverri, Xavier Iglesias, Thorsten Schuller, Uwe Hoffmann
To assess the validity of postexercise measurements to estimate oxygen uptake (V˙O2) during swimming, we compared V˙O2 measured directly during an all-out 200-m swim with measurements estimated during 200-m and 400-m maximal tests using several methods, including a recent heart rate (HR)/V˙O2 modelling procedure. 25 elite swimmers performed a 200-m maximal swim where V˙O2 was measured using a swimming snorkel connected to a gas analyzer. The criterion variable was V˙O2 in the last 20 s of effort, which was compared with the following V˙O2peak estimates: 1) first 20-s average; 2) linear backward extrapolation (BE) of the first 20 and 30 s, 3×20-s, 4×20-s, and 3×20-s or 4×20-s averages; 3) semilogarithmic BE at the same intervals; and 4) predicted V˙O2peak using mathematical modelling of 0-20 s and 5-20 s during recovery...
June 2017: International Journal of Sports Medicine
https://www.readbyqxmd.com/read/28479422/management-of-difficult-access-during-endovascular-aneurysm-repair
#18
Yana Etkin, Asad Baig, Paul J Foley, Grace J Wang, Edward Y Woo, Jeffrey P Carpenter, Ronald M Fairman, Benjamin M Jackson
BACKGROUND: To describe a large single-institutional experience in managing challenging access situations during endovascular aneurysm repair (EVAR). METHODS: Data from all patients undergoing EVAR at a tertiary academic medical center between 2009 and 2013 were collected retrospectively, including demographics, size of iliac arteries, type of device used, approach to managing difficult access (DA), and outcomes. The median follow-up was 38 months. DA was defined as iliac arteries with a diameter of less than 7 mm bilaterally...
October 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28475965/how-could-discharge-management-affect-florida-spring-fish-assemblage-structure
#19
Kirsten Work, Keneil Codner, Melissa Gibbs
Freshwater bodies are increasingly affected by reductions in water quantity and quality and by invasions of exotic species. To protect water quantity and maintain the ecological integrity of many water bodies in central Florida, a program of adopting Minimum Flows and Levels (MFLs) has begun for both lentic and lotic waters. The purpose of this study was to determine whether there were relationships between discharge and stage, water quality, and biological parameters for Volusia Blue Spring, a first magnitude spring (discharge > 380,000 m(3) day(-1) or 100 mgd) for which an MFL program was adopted in 2006...
August 1, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28464277/forensic-case-reports-presenting-immersion-pulmonary-edema-as-a-differential-diagnosis-in-fatal-diving-accidents
#20
Julie Vinkel, Peter Bak, Peter Juel Thiis Knudsen, Ole Hyldegaard
Immersion Pulmonary Edema (IPE) reduces the transport of gases over the respiratory membrane due to edema in the interstitium and respiratory zones. IPE has previously been described in both swimmers and divers, with a few known fatal cases. We have reviewed 42 SCUBA and snorkeling-related drowning deaths, and through a thorough analysis of each case, including both diving physiology and forensic pathology, we present IPE as a differential diagnosis to drowning in four cases. Our findings propose that; absence of watery content in the stomach and conducting airways, and liquid filled lungs without hyperexpansion, may be compatible with IPE...
May 2, 2017: Journal of Forensic Sciences
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