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https://www.readbyqxmd.com/read/29339159/analysis-of-the-interactions-between-gmf-and-arp2-3-complex-in-two-binding-sites-by-molecular-dynamics-simulation
#1
A Popinako, M Antonov, D Dibrova, A Chemeris, O S Sokolova
The Arp2/3 complex plays a key role in nucleating actin filaments branching. The glia maturation factor (GMF) competes with activators for interacting with the Arp2/3 complex and initiates the debranching of actin filaments. In this study, we performed a comparative analysis of interactions between GMF and the Arp2/3 complex and identified new amino acid residues involved in GMF binding to the Arp2/3 complex at two separate sites, revealed by X-ray and single particle EM techniques. Using molecular dynamics simulations we demonstrated the quantitative and qualitative changes in hydrogen bonds upon binding with GMF...
January 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29310060/generation-of-a-gde-heterozygous-mutation-human-embryonic-stem-cell-line-wae001-a-14-by-crispr-cas9-editing
#2
Guosheng Xu, Dongsheng Guo, Feima Wu, Nasir Abbas, Keyu Lai, Fang Yuan, Kai You, Yanli Liu, Yuanqi Zhuang, Yuhang Wu, Yingying Xu, Yan Chen, Fan Yang, Tingcai Pan, Yin-Xiong Li
Glycogen debranching enzyme (GDE) plays a critical role in glycogenolysis. Mutations in the GDE gene are associated with a metabolic disease known as glycogen storage disease type III (GSDIII). We generated a mutant GDE human embryonic stem cell line, WAe001-A-14, using the CRISPR/Cas9 editing system. This cell line contains a 24-nucleotide deletion within exon-13 of GDE, resulting in 8 amino acids (TRLGISSL) missing of the GDE protein from amino acid position 567 to 575. The WAe001-A-14 cell line maintains typical stem cell morphology, pluripotency and in vitro differentiation potential, and a normal karyotype...
December 13, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29307224/-thoracic-aortic-stentgraft-implantations-in-hungary-from-2012-to-2016
#3
Daniele Mariastefano Fontanini, Gábor Fazekas, Gábor Vallus, György Juhász, Rita Váradi, Zsolt Kövesi, Márton Kolossváry, Zoltán Szeberin
Thoracic aortic endograft implantation has become a widespread procedure in recent years, yet no report is available about Hungarian outcomes. Examination of our results is crucial to define further treatment strategies. Analysis of perioperative data from Hungarian thoracic endograft implantations based on the experience of 5 years is presented. Our retrospective, multicentric study analysed voluntarily reported data from all Hungarian institutions where thoracic endograft implantations are performed. Information was collected from every procedure performed in 5 years...
January 2018: Orvosi Hetilap
https://www.readbyqxmd.com/read/29249769/open-conversion-for-type-a-ascending-aortic-dissection-after-thoracic-endovascular-aortic-repair-by-the-chimney-graft-technique
#4
Hirofumi Kasahara, Yoshito Inoue, Takashi Hachiya
An 83-year-old man with aortic arch aneurysm underwent zone 0 thoracic endovascular aortic repair (TEVAR) by the chimney graft technique with two supra-aortic arch debranching grafts and developed subacute type A ascending aortic dissection. We performed emergency open conversion with circulatory arrest under deep hypothermia. The tip of the chimney graft (around the sino-tubular junction in the ascending aorta) was stiff, making it difficult to inspect the lumen and perform anastomosis. Deep hypothermic circulatory arrest and cutting the endograft stents to mobilize the graft were necessary for secure anastomosis...
December 18, 2017: Annals of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/29240061/-hybrid-treatment-of-patients-with-aneurysms-and-dissections-of-the-aortic-arch-and-descending-portion-of-the-thoracic-aorta
#5
V V Shlomin, M L Gordeev, D A Zverev, E A Shloĭdo, V E Uspenskiĭ, E D Zvereva, P B Bondarenko, P D Puzdriak
The authors share herein their experience with hybrid surgical treatment of 21 patients presenting with lesions of the aortic arch and descending thoracic aorta. Aortic pathology included dissection of the thoracoabdominal aorta (n=15), a sacciform aneurysm of the aortic arch (n=5), and a spindle-shaped aneurysm of the distal portions of the aortic arch (n=1). The first stage consisted of the following operations: transposition of the left subclavian artery into the left common carotid artery (n=9; 42.8%), partial debranching (n=11; 52...
2017: Angiologii︠a︡ i Sosudistai︠a︡ Khirurgii︠a︡, Angiology and Vascular Surgery
https://www.readbyqxmd.com/read/29238868/early-experience-and-lessons-learnt-with-customized-fenestrated-thoracic-endovascular-aortic-reconstruction-for-aortic-arch-pathology-in-an-asian-population
#6
Glenn Wei Leong Tan, Lawrence Quek, Bien Peng Tan, Uei Pua
PURPOSE: Thoracic endovascular aortic repair (TEVAR) is the preferred treatment of descending thoracic aorta pathology. However, TEVAR in the aortic arch remains challenging. Customized fenestrated TEVAR (fTEVAR) allows extension of the proximal seal zone, while preserving the involved supra-aortic vessels. There is a paucity of information on fTEVAR; hence, we aim to evaluate our early experience and lessons learnt with fTEVAR in the aortic arch. METHODS: This is a retrospective single-centre review of fTEVAR endografts for aortic arch pathology over 24 months...
December 13, 2017: Cardiovascular and Interventional Radiology
https://www.readbyqxmd.com/read/29237361/hybrid-endovascular-aortic-arch-reconstruction-for-acute-aortic-dissection-an-endovascular-bridge-technique-for-complex-anatomy
#7
Michael P Rogers, Sophie M Reskin, Adam Ubert, Matthew C Black, Kendra J Grubb
Acute type A aortic dissections starting at the arch pose a challenge for cardiac surgeons. Open surgical repair requires deep hypothermic circulatory arrest for arch reconstruction and is associated with significant morbidity and mortality. Hybrid aortic repair techniques, with open arch debranching and thoracic endovascular aortic repair, have been employed in high-risk cases and challenging aortic pathology. Herein, we present a case of a 33-year-old African American male with a history of open thoracoabdominal aortic reconstruction and femoral-femoral artery bypass for a type B dissection who subsequently presented with new-onset chest pain and was found to have a retrograde type A dissection of a bovine arch with multiple dissection flaps and possible contrast extravasation on chest computed tomography...
January 1, 2017: Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/29211214/hybrid-approach-of-aortic-diseases-zone-1-delivery-and-volumetric-analysis-on-the-descending-aorta
#8
José Augusto Duncan, Ricardo Ribeiro Dias, Fabrício José Dinato, Fábio Fernandes, Félix José Álvares Ramirez, Charles Mady, Fabio Biscegli Jatene
INTRODUCTION: Conventional techniques of surgical correction of arch and descending aortic diseases remains as high-risk procedures. Endovascular treatments of abdominal and descending thoracic aorta have lower surgical risk. Evolution of both techniques - open debranching of the arch and endovascular approach of the descending aorta - may extend a less invasive endovascular treatment for a more extensive disease with necessity of proximal landing zone in the arch. OBJECTIVE: To evaluate descending thoracic aortic remodeling by means of volumetric analysis after hybrid approach of aortic arch debranching and stenting the descending aorta...
September 2017: Brazilian Journal of Cardiovascular Surgery
https://www.readbyqxmd.com/read/29197821/penicillium-purpurogenum-produces-a-novel-endo-1-5-arabinanase-active-on-debranched-arabinan-short-arabinooligosaccharides-and-on-the-artificial-substrate-p-nitrophenyl-arabinofuranoside
#9
Felipe Vilches, María Cristina Ravanal, Felipe Bravo-Moraga, Danilo Gonzalez-Nilo, Jaime Eyzaguirre
Penicillium purpurogenum secretes numerous lignocellulose-degrading enzymes, including four arabinofuranosidases and an exo-arabinanase. In this work, the biochemical properties of an endo-arabinanase (ABN1) are presented. A gene, coding for a potential ABN was mined from the genome. It includes three introns. The cDNA is 975 bp long and codes for a mature protein of 324 residues. The cDNA was expressed in Pichia pastoris. The enzyme is active on debranched arabinan and arabinooligosaccharides. In contrast to other characterized ABNs, inactive on p-nitrophenyl-α-L-arabinofuranoside (pNPAra), ABN1 is active on this substrate...
November 26, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/29170828/a-novel-trifunctional-family-gh10-enzyme-from-acidothermus-cellulolyticus-11b-exhibiting-endo-xylanase-arabinofuranosidase-and-acetyl-xylan-esterase-activities
#10
Saher Shahid, Razia Tajwar, Muhammad Waheed Akhtar
A novel, family GH10 enzyme, Xyn10B from Acidothermus cellulolyticus 11B was cloned and expressed in Escherichia coli. This enzyme was purified to homogeneity by binding to regenerated amorphous cellulose. It had higher binding on Avicel as compared to insoluble xylan due to the presence of cellulose-binding domains, CBM3 and CBM2. This enzyme was optimally active at 70 °C and pH 6.0. It was stable up to 70 °C while the CD spectroscopy analysis showed thermal unfolding at 80 °C. Xyn10B was found to be a trifunctional enzyme having endo-xylanase, arabinofuranosidase and acetyl xylan esterase activities...
November 23, 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29163444/progressive-colonization-of-bacteria-and-degradation-of-rice-straw-in-the-rumen-by-illumina-sequencing
#11
Yanfen Cheng, Ying Wang, Yuanfei Li, Yipeng Zhang, Tianyi Liu, Yu Wang, Thomas J Sharpton, Weiyun Zhu
The aim of this study was to improve the utilization of rice straw as forage in ruminants by investigating the degradation pattern of rice straw in the dairy cow rumen. Ground up rice straw was incubated in situ in the rumens of three Holstein cows over a period of 72 h. The rumen fluid at 0 h and the rice straw at 0.5, 1, 2, 4, 6, 12, 24, 48, and 72 h were collected for analysis of the bacterial community and the degradation of the rice straw. The bacterial community and the carbohydrate-active enzymes in the rumen fluid were analyzed by metagenomics...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29153821/custom-made-e-vita-graft-for-frozen-elephant-trunk-with-arch-first-technique
#12
Luca Bertoglio, Alessandro Castiglioni, Alessandro Grandi, Tommaso Cambiaghi, Alessandro Verzini, Roberto Chiesa
A novel custom-made E-Vita Open prosthesis (JOTEC GmbH, Hechingen, Germany) has been designed with 2 additional side branches. The first one, the reperfusion branch, allows distal aortic perfusion after the collar anastomosis of a frozen elephant trunk repair has been performed. The second one, the debranching branch, permits reimplantation of any configuration of supraaortic debranching to the dacron graft. This modified graft provides two main advantages: first, combined with prior carotid-subclavian bypass and bilateral axillary perfusion, it allows continuous bihemispheric antegrade perfusion and arch-first technique under mild hypothermia (32°C); second, it grants distal aortic reperfusion after distal anastomosis completion performed under moderate hypothermia (28°C)...
December 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/29120761/complex-formation-between-starch-and-stearic-acid-effect-of-enzymatic-debranching-for-starch
#13
Chagam Koteswara Reddy, So Mang Choi, Dong-Jin Lee, Seung-Taik Lim
Effect of debranching for a high-amylose starch (∼70% amylose) on its V-complex formation with stearic acid was examined. Gel-permeation chromatograms showed that amylopectin was degraded to smaller molecules as the debranching time increased from 6 to 24 h. Increased formation of debranched starch/stearic acid complexes (recovery yield of stearic acid from 45.17 to 89.31% and starch from 39.92 to 55.43%) was observed with increased debranching time (from 6 to 24 h) and complexation time (from 6 to 24 h)...
April 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29101586/a-lariat-derived-circular-rna-is-required-for-plant-development-in-arabidopsis
#14
Jinping Cheng, Yong Zhang, Ziwei Li, Taiyun Wang, Xiaotuo Zhang, Binglian Zheng
Lariat RNA is produced during pre-mRNA splicing, and it is traditionally thought as by-products, due to the quick turnover by debranching followed by degradation. However, recent findings identified many lariat RNAs accumulate with a circular form in higher eukaryotes. Although the remarkable accumulation, biological consequence of lariat-derived circular RNAs (here we name laciRNAs) remains largely unknown. Here, we report that a specific laciRNA from At5g37720 plays an essential role in plant development by regulating gene expression globally...
October 31, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/29049788/full-metal-jacket-transfemoral-aortic-valve-implantation-for-regurgitant-valve-after-endovascular-aortic-repair%C3%A2
#15
Omer Tanyeli, Yuksel Dereli, Niyazi Gormus, Mehmet Akif Duzenli
Transfemoral aortic valve implantation has become an almost routine interventional procedure for severe aortic stenosis in high-risk patients. Over time an increased number of experiences has led to unusual procedures. In this report, we present a successful valve-in-valve transfemoral aortic valve implantation in a patient with aortic regurgitation, who previously had debranching and thoracic endovascular aortic repair operations.
July 25, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29049664/acute-type-i-aortic-dissection-a-propensity-matched-comparison-of-elephant-trunk-and-arch-debranching-repairs
#16
Mingjia Ma, Xin Feng, Jing Wang, Yiming Dong, Taiqiang Chen, Ligang Liu, Xiang Wei
OBJECTIVES: Our goal was to compare the performance of the frozen elephant trunk (FET) and the hybrid aortic arch debranching procedures for acute Type I aortic dissection. METHODS: From January 2013 to December 2015, 168 patients with Type I aortic disease underwent ascending aorta and total aortic arch replacement with FET implantation (the FET group, n  = 132) or arch debranching with 1-stage aortic arch exclusion using an endovascular stent in a retrograde manner (the debranching group, n  = 36)...
September 11, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29037665/unit-and-internal-chain-profiles-of-maca-amylopectin
#17
Ling Zhang, Guantian Li, Weirong Yao, Fan Zhu
Unit chain length distributions of amylopectin and its φ, β-limit dextrins, which reflect amylopectin internal structure from three maca starches, were determined by high-performance anion-exchange chromatography with pulsed amperometric detection after debranching, and the samples were compared with maize starch. The amylopectins exhibited average chain lengths ranging from 16.72 to 17.16, with ranges of total internal chain length, external chain length, and internal chain length of the maca amylopectins at 12...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29034019/hybrid-repair-of-an-abdominal-aortic-aneurysm-debranching-with-endovascular-aneurysm-repair-in-a-patient-with-horseshoe-kidney
#18
Keiwa Kin, Hiroshi Takano, Takaya Nakagawa, Yukitoshi Shirakawa
Abdominal aortic aneurysm (AAA) with associated horseshoe kidney (HSK) poses a technical challenge when performing conventional open surgical repair because of possible complications including renal infarction, neuralgia, and collecting system disruption. Endovascular aortic repair (EVAR) is considered the first-line treatment for this pathology, allowing for aneurysm repair without isthmus bisection. However, whether to sacrifice commonly presenting aberrant renal arteries during EVAR is a point of controversy...
March 24, 2017: Annals of Vascular Diseases
https://www.readbyqxmd.com/read/28966086/actin-turnover-in-lamellipodial-fragments
#19
Dikla Raz-Ben Aroush, Noa Ofer, Enas Abu-Shah, Jun Allard, Oleg Krichevsky, Alex Mogilner, Kinneret Keren
Actin turnover is the central driving force underlying lamellipodial motility. The molecular components involved are largely known, and their properties have been studied extensively in vitro. However, a comprehensive picture of actin turnover in vivo is still missing. We focus on fragments from fish epithelial keratocytes, which are essentially stand-alone motile lamellipodia. The geometric simplicity of the fragments and the absence of additional actin structures allow us to characterize the spatiotemporal lamellipodial actin organization with unprecedented detail...
October 9, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28931690/conformational-changes-in-the-5-end-of-the-hiv-1-genome-dependent-on-the-debranching-enzyme-dbr1-during-early-stages-of-infection
#20
Alvaro E Galvis, Hugh E Fisher, Hung Fan, David Camerini
Previous studies in our laboratory showed that the RNA debranching enzyme (DBR1) is not required for early steps in HIV cDNA formation but is necessary for synthesis of intermediate and late cDNA products. To further characterize this effect, we evaluated the topology of the 5' end of the HIV-1 RNA genome during early infection with and without inhibition of DBR1 synthesis. Cells were transfected with DBR1 short hairpin RNA (shRNA) followed 48 h later by infection with an HIV-1-derived vector containing an RNase H-deficient reverse transcriptase (RT)...
December 1, 2017: Journal of Virology
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