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Kavitha Odathurai Marusamy, Saravanan Ramasamy, Othman Wali
Early loss of permanent teeth, congenitally missing lateral incisors or second premolars, extremely displaced or impacted canines, all result in a reduced upper dentition. That leads to the development of crossbite in the maxillary dentition. Partial denture prosthesis, dental implants, and space closure by fixed orthodontics are the various means of treating the edentulous spaces. Protraction of molars is time taking and challenging. Temporary anchorage device (TAD) helps to protract molars without anchorage loss...
May 2018: Journal of International Society of Preventive & Community Dentistry
Tad M Mabry
BACKGROUND: Revision total hip arthroplasty for the treatment of taper corrosion has become increasingly common. The purpose of this portion of the symposium was to describe the most common complications of this procedure and to delineate specific measures that might be taken to prevent these complications. METHODS: A literature review was performed focusing on the results and complications of revision total hip arthroplasty performed for taper corrosion. RESULTS: The most common modes of failure after revision total hip arthroplasty in the setting of taper corrosion include the 4 I's: infection, instability, implant loosening, and ions (recurrent adverse local tissue reaction)...
May 29, 2018: Journal of Arthroplasty
Eline Overwater, Luisa Marsili, Marieke J H Baars, Annette F Baas, Irma van de Beek, Eelco Dulfer, Johanna M van Hagen, Yvonne Hilhorst-Hofstee, Marlies Kempers, Ingrid P Krapels, Leonie A Menke, Judith M A Verhagen, Kak K Yeung, Petra J G Zwijnenburg, Maarten Groenink, Peter van Rijn, Marjan M Weiss, Els Voorhoeve, J Peter van Tintelen, Arjan C Houweling, Alessandra Maugeri
Simultaneous analysis of multiple genes using next generation sequencing (NGS) technology has become widely available. Copy number variations (CNVs) in disease-associated genes have emerged as a cause for several hereditary disorders. CNVs are, however, not routinely detected using NGS analysis. The aim of this study was to assess the diagnostic yield and the prevalence of CNVs using our panel of Hereditary Thoracic Aortic Disease (H-TAD) associated genes. 810 patients suspected of H-TAD were analysed by targeted NGS analysis of 21 H-TAD associated genes...
June 16, 2018: Human Mutation
Mingming Yang, Dmitri Mavrodi, Linda S Thomashow, David M Weller
2,4-diacetylphloroglucinol (DAPG)-producing Pseudomonas spp. in the P. fluorescens complex are primarily responsible for a natural suppression of take-all of wheat known as take-all decline (TAD) in many fields in the USA. P. brassicacearum, the most common DAPG producer found in TAD soils in the Pacific Northwest (PNW) of the USA, has biocontrol, growth promoting and phytotoxic activities. In this study, we explored how the wheat cultivar affects the level of take-all suppression when grown in a TAD soil, and how cultivars respond to colonization by P...
June 15, 2018: Phytopathology
Salih Kumru, Hasan C Tekedar, Matt J Griffin, Geoffrey C Waldbieser, Mark R Liles, Tad Sonstegard, Steven G Schroeder, Mark L Lawrence, Attila Karsi
Aeromonas bestiarum is a Gram-negative mesophilic motile bacterium causing acute hemorrhagic septicemia or chronic skin ulcers in fish. Here, we report the draft genome sequence of A. bestiarum strain GA97-22, which was isolated from rainbow trout in 1997. This genome sequence will improve our understanding of the complex taxonomy of motile aeromonads.
June 14, 2018: Genome Announcements
Joachim Wolff, Vivek Bhardwaj, Stephan Nothjunge, Gautier Richard, Gina Renschler, Ralf Gilsbach, Thomas Manke, Rolf Backofen, Fidel Ramírez, Björn A Grüning
Galaxy HiCExplorer is a web server that facilitates the study of the 3D conformation of chromatin by allowing Hi-C data processing, analysis and visualization. With the Galaxy HiCExplorer web server, users with little bioinformatic background can perform every step of the analysis in one workflow: mapping of the raw sequence data, creation of Hi-C contact matrices, quality assessment, correction of contact matrices and identification of topological associated domains (TADs) and A/B compartments. Users can create publication ready plots of the contact matrix, A/B compartments, and TADs on a selected genomic locus, along with additional information like gene tracks or ChIP-seq signals...
June 13, 2018: Nucleic Acids Research
Anusha Poosapati, Emily Gregory, Wade M Borcherds, Lucia B Chemes, Gary W Daughdrill
The relationship between helical stability and binding affinity was examined for the intrinsically disordered transactivation domain (TAD) of the myeloblastosis oncoprotein, c-Myb and its ordered binding partner, KIX. A series of c-Myb mutants was designed to either increase or decrease helical stability without changing the binding interface with KIX. This included a complimentary series of A, G, P, and V mutants at three non-interacting sites. We were able to use the glycine mutants as a reference state and show a strong correlation between binding affinity and helical stability...
June 8, 2018: Journal of Molecular Biology
Chen-Yu Wang, Teddy Jégu, Hsueh-Ping Chu, Hyun Jung Oh, Jeannie T Lee
Mammalian chromosomes are partitioned into A/B compartments and topologically associated domains (TADs). The inactive X (Xi) chromosome, however, adopts a distinct conformation without evident compartments or TADs. Here, through exploration of an architectural protein, structural-maintenance-of-chromosomes hinge domain containing 1 (SMCHD1), we probe how the Xi is reconfigured during X chromosome inactivation. A/B compartments are first fused into "S1" and "S2" compartments, coinciding with Xist spreading into gene-rich domains...
May 28, 2018: Cell
Emine Müge Acar, Murat Tad, Asuman Kilitci, Funda Kemeriz
We report a case of hemosiderotic dermatofibroma presenting as a brown-black-colored nodule with peripheral extensions, which mimics melanoma. Histopathology showed completely benign features with no atypia or mitosis. Nodular extensions of childhood dermatofibromas may be related to the growth of the child not necessarily pointing to a malignant process.
June 2018: Clinical Case Reports
Devin M Jagow, Shahan V Yacoubian, Edward J McCrink, Stephan V Yacoubian
One-hundred and three patients with intertrochanteric hip fractures as classified by the OTA 2007 system underwent fixation using the telescoping AOS Galileo lag screw system and ES Nail (a long IM nail). Thirty-one patients (76%) were female and ten (24%) were male, with an average age of 75.70 ± 11.3 years at date of surgery. At final follow-up 21 (53%) patients returned to their pre-fracture ambulatory status, 20 (47%) had a reduction in ambulatory status, and no patients were confined to a wheel chair...
June 2018: Journal of Orthopaedics
Emma Åberg, O Andreas Karlsson, Eva Andersson, Per Jemth
Because of their prominent roles in cell cycle regulation and cancer the interaction between MDM2 and the intrinsically disordered transactivation domain (TAD) of p53 is exceptionally well studied. However, while there are numerous computational studies on the interaction mechanism there is a paucity of experimental data regarding the kinetics and mechanism. We have used stopped flow fluorescence to investigate the binding reaction between MDM2 and the TAD from p53 as well as from its paralogs p63 and p73, and in particular focussed on the salt dependence of the interaction...
June 7, 2018: Journal of Physical Chemistry. B
Lucas F daSilva, Felipe C Beckedorff, Ana C Ayupe, Murilo S Amaral, Vinícius Mesel, Alexandre Videira, Eduardo M Reis, João C Setubal, Sergio Verjovski-Almeida
Cell signaling events triggered by androgen hormone in prostate cells is dependent on activation of the androgen receptor (AR) transcription factor. Androgen hormone binding to AR promotes its displacement from the cytoplasm to the nucleus and AR binding to DNA motifs, thus inducing activatory and inhibitory transcriptional programs through a complex regulatory mechanism not yet fully understood. In this work, we performed RNA-seq deep-sequencing of LNCaP prostate cancer cells and found over 7000 expressed long intergenic non-coding RNAs (lincRNAs), of which ∼4000 are novel lincRNAs, and 258 lincRNAs have their expression activated by androgen...
2018: Frontiers in Genetics
Alvaro Rada-Iglesias, Frank G Grosveld, Argyris Papantonis
The last decade has radically renewed our understanding of higher order chromatin folding in the eukaryotic nucleus. As a result, most current models are in support of a mostly hierarchical and relatively stable folding of chromosomes dividing chromosomal territories into A- (active) and B- (inactive) compartments, which are then further partitioned into topologically associating domains (TADs), each of which is made up from multiple loops stabilized mainly by the CTCF and cohesin chromatin-binding complexes...
June 1, 2018: Molecular Systems Biology
Xiaolong Wang, Jing Liu, Yiyuan Niu, Yan Li, Shiwei Zhou, Chao Li, Baohua Ma, Qifang Kou, Bjoern Petersen, Tad Sonstegard, Xingxu Huang, Yu Jiang, Yulin Chen
BACKGROUND: The simplicity of the CRISPR/Cas9 system has enabled its widespread applications in generating animal models, functional genomic screening and in treating genetic and infectious diseases. However, unintended mutations produced by off-target CRISPR/Cas9 nuclease activity may lead to negative consequences. Especially, a very recent study found that gene editing can introduce hundreds of unintended mutations into the genome, and have attracted wide attention. RESULTS: To address the off-target concerns, urgent characterization of the CRISPR/Cas9-mediated off-target mutagenesis is highly anticipated...
May 25, 2018: BMC Genomics
Min Wang, Timothy P Hancock, Amanda J Chamberlain, Christy J Vander Jagt, Jennie E Pryce, Benjamin G Cocks, Mike E Goddard, Benjamin J Hayes
BACKGROUND: Topological association domains (TADs) are chromosomal domains characterised by frequent internal DNA-DNA interactions. The transcription factor CTCF binds to conserved DNA sequence patterns called CTCF binding motifs to either prohibit or facilitate chromosomal interactions. TADs and CTCF binding motifs control gene expression, but they are not yet well defined in the bovine genome. In this paper, we sought to improve the annotation of bovine TADs and CTCF binding motifs, and assess whether the new annotation can reduce the search space for cis-regulatory variants...
May 24, 2018: BMC Genomics
Wentian Sun, Kai Xia, Xinqi Huang, Xiao Cen, Qing Liu, Jun Liu
BACKGROUND: To investigate the feasibility, safety and stability of current interventions for moving teeth through the maxillary sinus (MTTMS) by performing a systematic review of the literature. METHODS: The electronic databases PubMed, Embase, CENTRAL, Web of Science, CBM, CNKI and SIGLE were searched without a language restriction. The primary outcomes were parameters related to orthodontic treatment, including orthodontic protocols, magnitude of forces, type of tooth movement, duration and rate of tooth movement, and remolding of alveolar bone and the maxillary sinus floor...
May 23, 2018: BMC Oral Health
Ying Cheng, Ziyi Li, Sasicha Manupipatpong, Li Lin, Xuekun Li, Tianlei Xu, Yong-Hui Jiang, Qiang Shu, Hao Wu, Peng Jin
Autism spectrum disorders (ASD) include a group of syndromes characterized by impaired language, social, and communication skills, in addition to restrictive behaviors or stereotypes. However, with a prevalence of 1.5% in developed countries and high comorbidity rates, no clear underlying mechanism that unifies the heterogeneous phenotypes of ASD exists. 5-hydroxymethylcytosine (5hmC) is highly enriched in the brain and recognized as an essential epigenetic mark in developmental and brain disorders. To explore the role of 5hmC in ASD, we used the genomic DNA isolated from the postmortem cerebellum of both ASD patients and age-matched controls to profile genome-wide distribution of 5hmC...
May 22, 2018: Human Molecular Genetics
Mahmut Kalem, Kerem Başarır, Hakan Kocaoğlu, Ercan Şahin, Hakan Kınık
Objectives: To examine the effect of fluoroscopy devices with different sizes of image intensifier and C-arm maneuverability on operating time, fluoroscopy time, radiation dose and reduction, and fixation quality at intertrochanteric femoral fractures. Design: Single-center, randomized, prospective study. Setting: Academic Level I trauma hospital. Patients and Intervention: 34 patients treated with cephalomedullary nailing for a stable, intertrochanteric proximal femur fracture (OTA A1)...
2018: BioMed Research International
Peter W Krug, Talina Davis, Catherine O'Brien, Michael LaRocco, Luis L Rodriguez
In the event of an intentional or accidental incursion of a transboundary animal disease (TAD) virus into the US, a major concern to the meat industry would be the potential contamination of packing plants by processing infected animals. TAD agents such as foot and mouth disease virus (FMDV), African swine fever virus (ASFV) and classical swine fever virus (CSFV) are found in swine products such as blood and feces and are present in the tissues of infected animals. To test the disinfection of TAD viruses in a pork-packing environment, a previously developed disinfection assay was used to test two biocides currently used by industry sanitarians, against TAD viruses dried on industry relevant surfaces in saline or swine products...
June 2018: Veterinary Microbiology
Ramaswamy Kannappan, Saidulu Mattapally, Pooja A Wagle, Jianyi Zhang
The role of p53 transactivation domain (p53-TAD), a multifunctional and dynamic domain, on DNA repair and retaining DNA integrity in human iPS cells has never been studied. p53-TAD was knocked out in iPS cells using CRISPR/Cas9 and was confirmed by DNA sequencing. p53-TAD KO cells were characterized by: accelerated proliferation, decreased population doubling time, and unaltered Bcl2, BBC3, IGF1R, Bax and altered Mdm2, p21, and PIDD transcripts expression. In p53-TAD KO cells p53 regulated DNA repair proteins XPA, DNA polH and DDB2 expression were found to be reduced compared to p53-WT cells...
May 18, 2018: American Journal of Physiology. Heart and Circulatory Physiology
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