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https://www.readbyqxmd.com/read/29793181/exogenously-applied-spermidine-alleviates-photosynthetic-inhibition-under-drought-stress-in-maize-zea-mays-l-seedlings-associated-with-changes-in-endogenous-polyamines-and-phytohormones
#1
Lijie Li, Wanrong Gu, Jing Li, Congfeng Li, Tenglong Xie, Danyang Qu, Yao Meng, Caifeng Li, Shi Wei
Drought stress (DS) is a major environmental factor limiting plant growth and crop productivity worldwide. It has been established that exogenous spermidine (Spd) stimulates plant tolerance to DS. The effects of exogenous Spd on plant growth, photosynthetic performance, and chloroplast ultrastructure as well as changes in endogenous polyamines (PAs) and phytohormones were investigate in DS-resistant (Xianyu 335) and DS-sensitive (Fenghe 1) maize seedlings under well-watered and DS treatments. Exogenous Spd alleviated the stress-induced reduction in growth, photosynthetic pigment content, photosynthesis rate (Pn ) and photochemical quenching (qP ) parameters, including the maximum photochemistry efficiency of photosystem II (PSII) (Fv /Fm ), PSII operating efficiency (ФPSII), and qP coefficient...
May 15, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29793163/rheological-characterisation-of-concentrated-domestic-slurry
#2
A K Thota Radhakrishnan, J B van Lier, F H L R Clemens
The much over-looked element in new sanitation, the transport systems which bridge the source and treatment facilities, is the focus of this study. The knowledge of rheological properties of concentrated domestic slurry is essential for the design of the waste collection and transport systems. To investigate these properties, samples were collected from a pilot sanitation system in the Netherlands. Two types of slurries were examined: black water (consisting of human faecal waste, urine, and flushed water from vacuum toilets) and black water with ground kitchen waste...
May 3, 2018: Water Research
https://www.readbyqxmd.com/read/29793139/the-use-of-low-intensity-pulsed-ultrasound-in-treating-delayed-union-of-fifth-metatarsal-fractures
#3
Kar Hao Teoh, Robert Whitham, Jenny F Wong, Kartik Hariharan
BACKGROUND: There are no studies looking at the success rate of low-intensity pulsed ultrasound (LIPUS) in fifth metatarsal fracture delayed unions to our knowledge. The aim of this study is to investigate the use of LIPUS treatment for delayed union of fifth metatarsal fractures. METHODS: A retrospective review of patients who were treated with LIPUS following a delayed union of fifth metatarsal fracture was conducted over a three-year period. RESULTS: There were thirty patients (9 males, 21 females) in this cohort...
January 31, 2018: Foot
https://www.readbyqxmd.com/read/29793124/how-to-manage-patellofemoral-pain-understanding-the-multifactorial-nature-and-treatment-options
#4
Simon Lack, Bradley Neal, Danilo De Oliveira Silva, Christian Barton
Patellofemoral pain (PFP) is one of the most prevalent conditions within sports medicine, orthopaedic and general practice settings. Long-term treatment outcomes are poor, with estimates that more than 50% of people with the condition will report symptoms beyond 5 years following diagnosis. Additionally, emerging evidence indicates that PFP may be on a continuum with patellofemoral osteoarthritis. Consensus of world leading clinicians and academics highlights the potential benefit of delivering tailored interventions, specific to an individual's needs, to improve patient outcome...
April 12, 2018: Physical Therapy in Sport
https://www.readbyqxmd.com/read/29793096/segmentation-of-histological-images-and-fibrosis-identification-with-a-convolutional-neural-network
#5
Xiaohang Fu, Tong Liu, Zhaohan Xiong, Bruce H Smaill, Martin K Stiles, Jichao Zhao
Segmentation of histological images is one of the most crucial tasks for many biomedical analyses involving quantification of certain tissue types, such as fibrosis via Masson's trichrome staining. However, challenges are posed by the high variability and complexity of structural features in such images, in addition to imaging artifacts. Further, the conventional approach of manual thresholding is labor-intensive, and highly sensitive to inter- and intra-image intensity variations. An accurate and robust automated segmentation method is of high interest...
May 16, 2018: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/29793078/comparison-of-performance-based-assessment-and-real-world-skill-in-people-with-serious-mental-illness-ecological-validity-of-the-test-of-grocery-shopping-skills
#6
Laura A Faith, Melisa V Rempfer
Valid functional measures are essential for clinical and research efforts that address recovery and community functioning in people with serious mental illness. Although there is a great deal of interest in functional assessment, there is limited research supporting how well current evaluation methods provide a true assessment of real world functioning or naturalistic behavior. To address this gap in the literature, the present study examined the performance of individuals with serious mental illness (i.e., diagnosis of schizophrenia-spectrum, bipolar disorder, or other depression/anxiety diagnoses and accompanying functional disability) on the Test of Grocery Shopping Skills (TOGSS), a performance-based naturalistic task...
May 7, 2018: Psychiatry Research
https://www.readbyqxmd.com/read/29793072/leveraging-semantic-labels-for-multi-level-abstraction-in-medical-process-mining-and-trace-comparison
#7
Giorgio Leonardi, Manuel Striani, Silvana Quaglini, Anna Cavallini, Stefania Montani
Many medical information systems record data about the executed process instances in the form of an event log. In this paper, we present a framework, able to convert actions in the event log into higher level concepts, at different levels of abstraction, on the basis of domain knowledge. Abstracted traces are then provided as an input to trace comparison and semantic process discovery. Our abstraction mechanism is able to manage non trivial situations, such as interleaved actions or delays between two actions that abstract to the same concept...
May 21, 2018: Journal of Biomedical Informatics
https://www.readbyqxmd.com/read/29793070/intradialytic-blood-pressure-pattern-recognition-based-on-density-peak-clustering
#8
Feng Wang, Jing-Yi Zhou, Yu Tian, Yu Wang, Ping Zhang, Jiang-Hua Chen, Jing-Song Li
End-stage renal disease (ESRD) is the final stage of chronic kidney disease (CKD) and requires hemodialysis (HD) for survival. Intradialytic blood pressure (IBP) measurements are necessary to ensure patient safety during HD treatments and have critical clinical and prognostic significance. Studies on IBP measurements, especially IBP patterns, are limited. All related studies have been based on a priori knowledge and artificially classified IBP patterns. Therefore, the results were influenced by subjective concepts...
May 21, 2018: Journal of Biomedical Informatics
https://www.readbyqxmd.com/read/29793054/bacillus-clausii-inhibits-bone-loss-by-skewing-treg-th17-cell-equilibrium-in-postmenopausal-osteoporotic-mice-model
#9
Hamid Y Dar, Subhashis Pal, Prashant Shukla, Pradyumna K Mishra, Geetanjali B Tomar, Naibedya Chattopadhyay, Rupesh K Srivastava
OBJECTIVES: Postmenopausal osteoporosis is one of most commonly occurring skeletal diseases leading to bone loss and fragility. Probiotics have been associated with various immunomodulatory properties and thus can be exploited to enhance bone health. In the present study, we report, to our knowledge for the first time, that oral administration of Bacillus clausii (BC) in postmenopausal osteoporotic (OVX) mice model enhances bone health. METHODS: BC was selected as probiotic of choice due to its established immunomodulatory properties...
March 20, 2018: Nutrition
https://www.readbyqxmd.com/read/29793010/restoration-of-autologous-arteriovenous-fistula-by-removal-of-the-occluded-short-venous-part-and-venovenous-end-to-end-anastomosis-in-a-hemodialysis-patient
#10
Tsuyoshi Takashima, Keiichiro Matsumoto, Chihiro Takeshita, Eriko Nonaka, Sae Matsushita, Makoto Fukuda, Motoaki Miyazono, Yuji Ikeda
Standard salvage procedures for occuluded autologous arteriovenous fistula (AVF) in a hemodialysis patient are endovascular and/or surgical therapy. When endovascular therapy and thrombectomy prove unsuccessful, it is most likely that creating a new AVF or arteriovenous graft will be considered. However, if the occuluded venous part is short, we have adopted an operative technique for repair of AVF by removal of the occluded short venous part and venovenous end-to-end anastomosis. To our knowledge, the efficacy and clinical course of restoration of AVF by the technique have not been reported to date...
May 21, 2018: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/29792998/bmi-and-gender-increase-risk-for-sacral-fractures-after-multilevel-instrumented-spinal-fusion-compared-to-bone-mineral-density-and-pelvic-parameters
#11
Stephan N Salzmann, Courtney Ortiz Miller, John A Carrino, Jingyan Yang, Jennifer Shue, Andrew A Sama, Frank P Cammisa, Federico P Girardi, Alexander P Hughes
BACKGROUND CONTEXT: Sacral fractures are a rare but potentially devastating complication. Long-fusion constructs including the sacrum that do not extend to the pelvis may result in sacral fractures. Besides established risk factors including gender, age and number of levels fused, body mass index (BMI), pelvic parameters and bone mineral density (BMD) have also been proposed as potential risk factors for postoperative sacral fractures. The literature supporting this, however, is limited...
May 21, 2018: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/29792997/risk-factors-for-low-back-pain-and-sciatica-an-umbrella-review
#12
REVIEW
P Parreira, C Maher, D Steffens, M Hancock, M Ferreira
BACKGROUND: Low back pain (LBP) is a highly prevalent condition and it is associated with significant disability and work absenteeism worldwide. A variety of environmental and individual characteristics have been reported to increase the risk of low back pain (LBP). To our knowledge, there has been no previous attempt to summarise the evidence from existing systematic reviews of risk factors for LBP and/or sciatica PURPOSE: to provide an overview of risk factors for LBP, we completed an umbrella review of the evidence from existing systematic reviews...
May 21, 2018: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/29792995/lumbar-discectomy-is-associated-with-higher-rates-of-lumbar-fusion
#13
Hector Castillo, Ravi Chintapalli, Harout Boyajian, Sebastian A Cruz, Vince K Morgan, Lewis L Shi, Michael J Lee
BACKGROUND CONTEXT: Lumbar disc herniation affects over three million people in the United States every year, and the rate of operation continually increases, particularly in patients 60 years or older.1,2 Surgical discectomy is a common treatment for lumbar disc herniation.1,3 One concern for this method is the risk of undergoing additional surgeries. 2,5,7 There are very limited population-level studies that examine the rate of lumbar fusion following lumbar discectomy. Additionally, there is no study that examines the risk of undergoing lumbar fusion in patients who have undergone lumbar discectomies compared to the risk of lumbar fusion in the general population with no previous lumbar discectomy...
May 21, 2018: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/29792990/identification-of-group-a-rotaviruses-from-zambian-fruit-bats-provides-evidence-for-long-distance-dispersal-events-in-africa
#14
Michihito Sasaki, Masahiro Kajihara, Katendi Changula, Akina Mori-Kajihara, Hirohito Ogawa, Bernard M Hang'ombe, Aaron S Mweene, Martin Simuunza, Reiko Yoshida, Michael Carr, Yasuko Orba, Ayato Takada, Hirofumi Sawa
Group A rotavirus (RVA) is a major cause of diarrhea in children worldwide. Although RVA infects many animals, little is known about RVA in bats. The present study investigated the genetic diversity of RVA in Zambian bats. We identified RVA from two straw-colored fruit bats (Eidolon helvum) and an Egyptian fruit bat (Rousettus aegyptiacus), and analyzed the genome sequences of these strains. Genome segments of the RVA strains from Zambian E. helvum showed 97%-99% nucleotide sequence identity with those of other RVA strains from E...
May 21, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29792967/validated-reverse-phase-hplc-diode-array-method-for-the-quantification-of-intact-bevacizumab-infliximab-and-trastuzumab-for-long-term-stability-study
#15
Antonio Martínez-Ortega, Agustín Herrera, Antonio Salmerón-García, José Cabeza, Luis Cuadros-Rodríguez, Natalia Navas
The aim of the present study was to develop suitable and reliable method for quantification three of the most worldwide used therapeutic monoclonal antibodies (mAbs) -bevazizumab (BVZ), infliximab (INF) and trastuzumab (TTZ)- to be used in long-term stability studies. Reverse phase (RP) was selected by its greater sensibility and reproducibility comparing with other chromatographic modes. Then a high performance liquid chromatography with diode array detection (RP)HPLC/DAD method was checked. Since the three mAbs represent the active ingredient in the medicines in which they are formulated, the selected method was validated for each one in accordance with the International Conference on Harmonization (ICH) guidelines for pharmaceuticals for human use...
May 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29792943/the-development-of-a-screening-protocol-for-salmonella-spp-and-enteropathogenic-yersinia-spp-in-samples-from-wild-boar-sus-scrofa-also-generating-mlva-data-for-y-enterocolitica-and-y-pseudotuberculosis
#16
Axel Sannö, Magdalena Jacobson, Sandra Sterner, Susanne Thisted-Lambertz, Anna Aspán
Salmonellosis and yersiniosis are notifiable human diseases that are commonly associated with contaminated food. Domestic pigs as well as wild boars and other wild-life have been identified as reservoirs of these bacteria. Methods for cultivation and molecular epidemiological investigations of Salmonella spp. are well established, however, cultivation of enteropathogenic Yersinia spp. is time- consuming and the commonly used method for molecular epidemiological investigations, pulsed-field gel electrophoresis, lack in discriminatory power...
May 21, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29792937/a-reliable-targeted-next-generation-sequencing-strategy-for-diagnosis-of-myopathies-and-muscular-dystrophies-especially-for-the-giant-titin-and-nebulin-genes
#17
Reda Zenagui, Delphine Lacourt, Henri Pegeot, Kevin Yauy, Raul Juntas Morales, Corine Theze, François Rivier, Claude Cances, Guilhem Sole, Dimitri Renard, Ulrike Walther-Louvier, Xavier Ferrer-Monasterio, Caroline Espil, Marie-Christine Arné-Bes, Pascal Cintas, Emmanuelle Uro-Coste, Marie-Laure Martin Negrier, Valérie Rigau, Eric Bieth, Cyril Goizet, Mireille Claustres, Michel Koenig, Mireille Cossée
Myopathies and muscular dystrophies (M-MDs) are genetically heterogeneous diseases, with more than 100 identified genes, including the giant and complex titin (TTN) and nebulin (NEB) genes. Next-generation sequencing technology revolutionized M-MD diagnosis and revealed high frequency of TTN and NEB variants. We developed a next-generation sequencing diagnostic strategy targeted on the coding sequences of 135 M-MD genes. Comparison of two targeted capture technologies (SeqCap EZ Choice library capture kit (Roche-Nimblegen) and Nextera Rapid Capture Custom Enrichment kit (Illumina)) and of two whole exome sequencing kits (SureSelect V5 (Agilent) and TruSeq RapidExome capture (Illumina)) revealed best coverage with the SeqCap EZ Choice protocol...
May 21, 2018: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/29792892/beef-quality-labels-a-combination-of-sensory-acceptance-test-stated-willingness-to-pay-and-choice-based-conjoint-analysis
#18
Stephan G H Meyerding, Maria Gentz, Brianne Altmann, Lisa Meier-Dinkel
Consumer perspectives of beef quality are complex, leading to a market that is increasingly differentiating. Thus, ongoing monitoring and assessment of changes in consumer perspectives is essential to identify changing market conditions. Often only credence and search characteristics are evaluated in consumer studies; therefore the object of the present study is to examine consumer preferences and perceptions towards beef steaks, also including experience characteristics, using a mixed methods approach. For this reason, 55 consumers participated in an experiment in Germany, including a sensory acceptance test, stated willingness to pay, and choice-based conjoint analysis (CBCA)...
May 21, 2018: Appetite
https://www.readbyqxmd.com/read/29792886/t-grasp-a-targeted-grasp-for-assessing-neuronal-connectivity
#19
Harold K Shearin, Casey D Quinn, Robert D Mackin, Ian S Macdonald, R Steven Stowers
BACKGROUND: Understanding how behaviors are generated by neural circuits requires knowledge of the synaptic connections between the composite neurons. Methods for mapping synaptic connections, such as electron microscopy and paired recordings, are labor intensive and alternative methods are thus desirable. NEW METHOD: Development of a targeted GFP Reconstitution Across Synaptic Partners(GRASP) method, t-GRASP, for assessing neural connectivity is described. RESULTS: Numerous different pre-synaptic and post-synaptic/dendritic proteins were tested for enhancing the specificity of GRASP signal to synaptic regions...
May 21, 2018: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/29792815/molecular-mechanisms-of-fast-neurotransmitter-release
#20
Axel T Brunger, Ucheor B Choi, Ying Lai, Jeremy Leitz, Qiangjun Zhou
This review summarizes current knowledge of synaptic proteins that are central to synaptic vesicle fusion in presynaptic active zones, including SNAREs (soluble N-ethylmaleimide sensitive factor attachment protein receptors), synaptotagmin, complexin, Munc18 (mammalian uncoordinated-18), and Munc13 (mammalian uncoordinated-13), and highlights recent insights in the cooperation of these proteins for neurotransmitter release. Structural and functional studies of the synaptic fusion machinery suggest new molecular models of synaptic vesicle priming and Ca2+ -triggered fusion...
May 20, 2018: Annual Review of Biophysics
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