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MMP, TIMP, vagina

Agnieszka Leśniak-Walentyn, Anna Hrabia
Induced pause in egg laying simulates natural molting events in which the hen's reproductive organs regress and rejuvenate. Such processes require extracellular matrix remodeling that is maintained, at least in part, by the action of proteolytic enzymes known as matrix metalloproteinases (MMPs). Nevertheless, information concerning the expression and hormonal regulation of MMP system members in chickens is scarce. Therefore, MMP-2, -7, and -9 and their tissue inhibitors (TIMP-2, -3) expression and localization were investigated in all segments of the domestic hen oviduct (infundibulum, magnum, isthmus, shell gland, vagina) during a pause in egg laying induced by tamoxifen (TMX)-an estrogen receptor modulator...
January 15, 2017: Theriogenology
Agnieszka Leśniak-Walentyn, Anna Hrabia
This study was undertaken to examine mRNA and protein expression, localization of selected MMPs and their tissue inhibitors (TIMPs), and the activity of MMPs in all segments of the chicken oviduct (infundibulum, magnum, isthmus, shell gland, vagina) during a pause in egg laying induced by food deprivation. Control chickens (n = 18) were fed ad libitum, while experimental birds (n = 18) were fasted for 5 days, followed by being fed every other day, and then fed daily from day 10 onwards. Chickens were decapitated on day 6 (when the oviduct was regressed), day 13 (during oviduct recrudescence), and days 17-20 (oviduct rejuvenation) of the experiment...
November 2016: Cell and Tissue Research
May Alarab, Hala Kufaishi, Stephen Lye, Harold Drutz, Oksana Shynlova
AIM: The molecular etiology of pelvic organ prolapse (POP) is complex and not well understood. We compared the expression/activity of extracellular matrix (ECM)-processing (procollagen I N-proteinase/ a disintegrin and metalloproteinase with thrombospondin motifs [ADAMTS]-2,-3,-14) and ECM-degrading (matrix metalloproteinase [MMP]-1, -2, -7, -8, -9, -12) enzymes and their natural tissue inhibitors (tissue inhibitors of metalloproteinase [TIMP]-1,-2,-3,-4) in vaginal tissues from premenopausal women with advanced POP (POP-Q stage ≥ 3) and asymptomatic controls (POP-Q = 0)...
June 2014: Reproductive Sciences
Yujing J Heng, Megan K W Di Quinzio, Stella Liong, Michael Permezel, Gregory E Rice, Harry M Georgiou
Temporal expression of matrix metalloproteinase (MMP)-1, -2, -3, -7, -8, -9, -12, and -13, and tissue inhibitors of metalloproteinases (TIMPs)-1 and -2 in human cervicovaginal fluid (CVF) in term pregnancy and labor was investigated. Term parous women provided CVF samples that were grouped into labor, 1 to 3, 6 to 8, and 12 to 16 days before labor onset. Both MMPs and TIMPs (n = 60) were quantified using multiplex solution array and enzyme-linked immunosorbent assays, respectively. Further analysis of TIMP-1 (n = 180) was undertaken...
January 2012: Reproductive Sciences
S Ennen, S Kloss, G Scheiner-Bobis, K Failing, A Wehrend
The objectives of the present study were to evaluate the hormonal profiles, histology of the vagina and biomolecular analysis of connective tissue of ewes with and without vaginal prolapse. Blood samples from the jugular vein and biopsies of the vaginal tissue were taken from five late term pregnant, unaffected animals, four sheep during parturition and six ewes suffering from vaginal prolapse ante partum. The blood samples were submitted for determining the concentration of the steroid hormones progesterone by automatic luminescence immunoassay and estradiol-17β by the sequence test...
January 15, 2011: Theriogenology
Qun-Fang Zhang, Yan-Feng Song, Zhong-Yong Zhu
OBJECTIVE: To study semi-quantitatively mRNA expression of matrix metalloproteinase-9 (MMP-9) and its inhibitor, tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), in vaginal wall connective tissue in women with stress urinary incontinence (SUI) compared to continent controls, and to explore the relationship between MMP-9, TIMP-1 and SUI. METHODS: Vaginal wall tissues were obtained from 24 women with SUI who were followed-up (12 cases are > 60 years old and 12 cases < or = 60 years old)...
December 2006: Zhonghua Fu Chan Ke za Zhi
Christian H Phillips, Fred Anthony, Chris Benyon, Ash K Monga
OBJECTIVE: To compare tissue markers of collagen metabolism in the uterosacral ligaments with those in vaginal tissue in women with uterine prolapse. DESIGN: Prospective observational experimental study. SETTING: A tertiary urogynaecology unit. POPULATION: Women referred for hysterectomy for prolapse or benign gynaecological disease. METHODS: Matrix metalloproteinase (MMP)-2 and -9 expression, tissue inhibitors of metalloproteinase (TIMP)-2 expression and hydroxyproline content were measured in the uterosacral ligaments and vaginal tissue from 14 women with prolapse compared with 14 controls...
January 2006: BJOG: An International Journal of Obstetrics and Gynaecology
Tatsuro Kishida, Hideto Yamada, Itsuko Furuta, Noriko Kobayashi, Emi K Hirayama, Yasuhiko Ebina, Noriaki Sakuragi, Seiichiro Fujimoto
OBJECTIVE: The objective of this study was to investigate the predictive factors of premature rupture of the membranes (preterm PROM). METHODS: The study was undertaken with cervical secretions collected from 72 consenting singleton pregnant women between 20 and 33 weeks of gestation. The levels of interleukin (IL) 1alpha, IL-1beta, IL-6, IL-8, matrix metalloproteinase (MMP) 1, MMP-2, MMP-9, tissue inhibitors of matrix metalloproteinase (TIMP) 1, TIMP-2, granulocyte elastase, and fetal fibronectin in cervical diluted specimens were measured by immunoassay, and the uterine cervix was assessed by transvaginal ultrasonography...
March 2003: Fetal Diagnosis and Therapy
B H Chen, Y Wen, H Li, M L Polan
The aim of this study was to investigate quantitative mRNA expression of matrix metalloproteinases MMP-1, MMP-2, MMP-9, and their inhibitors, the tissue inhibitors of metalloproteinases TIMP-1, TIMP-2 and TIMP-3, in vaginal wall tissue from women with stress urinary incontinence compared to continent controls. Vaginal wall tissues were obtained from 7 women with stress urinary incontinence/severe pelvic prolapse and 15 continent controls. RNA was then extracted and quantified. Quantitative competitive reverse transcription (QC-RT-PCR) was carried out with oligonucleotide primers to quantify MMP-1, MMP-2, MMP-9, TIMP-1, TIMP-2 and TIMP-3 mRNA expression...
2002: International Urogynecology Journal and Pelvic Floor Dysfunction
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