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https://www.readbyqxmd.com/read/28655215/development-of-a-new-method-based-on-unmodified-gold-nanoparticles-and-peptide-nucleic-acids-for-detecting-bovine-viral-diarrhea-virus-rna
#1
Maryam Askaravi, Seyedeh Elham Rezatofighi, Saadat Rastegarzadeh, Masoud Reza Seifi Abad Shapouri
A simple colorimetric assay is presented for detecting bovine viral diarrhea virus (BVDV)-RNA based on aggregation of gold nanoparticles (AuNPs) in the presence of charge-neutral peptide nucleic acids (PNA). Free charge-neutral PNA oligomers tended to be adsorbed onto AuNPs and act as a coagulant, whereas hybridizing complementary RNA with PNA disrupted PNA-induced AuNP aggregation, and the NPs remained stable. However, non-complementary RNA did not have this effect, and PNA induced aggregation of the AuNPs that resulted in a color change of the reaction from red to blue...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28655072/oral-administration-of-glycine-and-leucine-dipeptides-improves-skin-hydration-and-elasticity-in-uvb-irradiated-hairless-mice
#2
Jong Il Park, Ji Eun Lee, Hyun Jun Shin, Soonran Song, Won Kyung Lee, Jae Sung Hwang
Placenta is a special organ that contains many nutrients such as growth factors, minerals, and bioactive peptides. Dipeptides of glycine and leucine are major components of porcine placenta extracts (PPE) that has been used as an alternative of human placenta extracts. In this study, we investigated whether major peptides of PPE, Glycyl-L-Leucine (Gly-Leu), L-Leucyl-Glycine (Leu-Gly), and L-Leucyl-L-Leucine (Leu-Leu), affect skin hydration and elasticity in vitro and in vivo. We found that Gly-Leu and Leu-Gly dipeptides induced the expression of transglutaminase 1 in normal human epidermal keratinocytes (NHEKs) whereas Leu-Leu dipeptides did not...
June 27, 2017: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/28654888/mek-inhibition-enhances-efficacy-of-bacillus-calmette-gu%C3%A3-rin-on-bladder-cancer-cells-by-reducing-release-of-toll-like-receptor-2-activated-antimicrobial-peptides
#3
Young Mi Whang, Su Bin Jin, Serk In Park, In Ho Chang
Bacillus Calmette-Guérin (BCG) is one of the standard treatment options for non-muscle-invasive bladder cancer. The details of the biological defense mechanisms against BCG remain unclear. Here, we investigated whether BCG-induced release of antimicrobial peptides (AMPs; e.g., human β-defensin-2, -3, and cathelicidin) is involved with mitogen-activated protein kinase (MAPK) pathways, and investigated the enhanced anticancer effect of BCG through the down-regulation of Toll-like receptors (TLRs) and MAPK pathways in bladder cancer cells...
May 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/28654884/specific-and-selective-probes-for-staphylococcus-aureus-from-phage-displayed-random-peptide-libraries
#4
Laura M De Plano, Santina Carnazza, Grazia M L Messina, Maria Giovanna Rizzo, Giovanni Marletta, Salvatore P P Guglielmino
Staphylococcus aureus is a major human pathogen causing health care-associated and community-associated infections. Early diagnosis is essential to prevent disease progression and to reduce complications that can be serious. In this study, we selected, from a 9-mer phage peptide library, a phage clone displaying peptide capable of specific binding to S. aureus cell surface, namely St.au9IVS5 (sequence peptide RVRSAPSSS).The ability of the isolated phage clone to interact specifically with S. aureus and the efficacy of its bacteria-binding properties were established by using enzyme linked immune-sorbent assay (ELISA)...
June 17, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28654861/aging-alters-mrna-expression-of-amyloid-transporter-genes-at-the-blood-brain-barrier
#5
Doreen Osgood, Miles C Miller, Arthur A Messier, Liliana Gonzalez, Gerald D Silverberg
Decreased clearance of potentially toxic metabolites, due to aging changes, likely plays a significant role in the accumulation of amyloid-beta (Aβ) peptides and other macromolecules in the brain of the elderly and in the patients with Alzheimer's disease (AD). Aging is the single most important risk factor for AD development. Aβ transport receptor proteins expressed at the blood-brain barrier are significantly altered with age: the efflux transporters lipoprotein receptor-related protein 1 and P-glycoprotein are reduced, whereas the influx transporter receptor for advanced glycation end products is increased...
May 19, 2017: Neurobiology of Aging
https://www.readbyqxmd.com/read/28654726/exploring-short-linear-motifs-using-the-elm-database-and-tools
#6
Marc Gouw, Hugo Sámano-Sánchez, Kim Van Roey, Francesca Diella, Toby J Gibson, Holger Dinkel
The Eukaryotic Linear Motif (ELM) resource is dedicated to the characterization and prediction of short linear motifs (SLiMs). SLiMs are compact, degenerate peptide segments found in many proteins and essential to almost all cellular processes. However, despite their abundance, SLiMs remain largely uncharacterized. The ELM database is a collection of manually annotated SLiM instances curated from experimental literature. In this article we illustrate how to browse and search the database for curated SLiM data, and cover the different types of data integrated in the resource...
June 27, 2017: Current Protocols in Bioinformatics
https://www.readbyqxmd.com/read/28654680/serum-fatty-acid-binding-protein-4-fabp4-concentration-is-associated-with-insulin-resistance-in-peripheral-tissues-a-clinical-study
#7
Risa Nakamura, Tsuyoshi Okura, Yohei Fujioka, Keisuke Sumi, Kazuhiko Matsuzawa, Shoichiro Izawa, Etsuko Ueta, Masahiko Kato, Shin-Ichi Taniguchi, Kazuhiro Yamamoto
Type 2 diabetes mellitus (T2DM) is caused by insulin resistance and β cell dysfunction. In recent studies reported that several markers associated with insulin sensitivity in skeletal muscle, Adiponectin and other parameters, such as fatty acid-binding protein (FABP4), have been reported to regulate insulin resistance, but it remains unclear which factor mostly affects insulin resistance in T2DM. In this cross-sectional study, we evaluated the relationships between several kinds of biomarkers and insulin resistance, and insulin secretion in T2DM and healthy controls...
2017: PloS One
https://www.readbyqxmd.com/read/28654665/probing-the-potential-of-cnab-type-domains-for-the-design-of-tag-catcher-systems
#8
Marlene Pröschel, Max E Kraner, Anselm H C Horn, Lena Schäfer, Uwe Sonnewald, Heinrich Sticht
Building proteins into larger, post-translational assemblies in a defined and stable way is still a challenging task. A promising approach relies on so-called tag/catcher systems that are fused to the proteins of interest and allow a durable linkage via covalent intermolecular bonds. Tags and catchers are generated by splitting protein domains that contain intramolecular isopeptide or ester bonds that form autocatalytically under physiological conditions. There are already numerous biotechnological and medical applications that demonstrate the usefulness of covalent linkages mediated by these systems...
2017: PloS One
https://www.readbyqxmd.com/read/28654661/structure-dynamics-and-kinetics-of-two-component-lantibiotic-lichenicidin
#9
Alejandra de Miguel, Rafael Tapia-Rojo, Tillmann Utesch, Maria Andrea Mroginski
Two variants of the two-component Lantibiotic Lichenicidin, produced by the strains B. Licheniformis VK21 and I89 (Lchα/ Lchβ and Bliα/ Bliβ peptides, respectively) have been investigated by means of 2 μs-long all-atom molecular dynamics simulations combined with Markov State Models. This rigorous statistical analysis enabled to evaluate the dynamic and kinetic properties of the aforementioned systems which are not accessible via experimental techniques. The structural flexibility characteristic of these small peptides is understood by a delicate equilibrium between random coil, α-helices and β-sheet structures...
2017: PloS One
https://www.readbyqxmd.com/read/28654654/identification-and-characterization-of-acka-dependent-protein-acetylation-in-neisseria-gonorrhoeae
#10
Deborah M B Post, Birgit Schilling, Lorri M Reinders, Alexandria K D'Souza, Margaret R Ketterer, Steven J Kiel, Aroon T Chande, Michael A Apicella, Bradford W Gibson
Neisseria gonorrhoeae, the causative agent of gonorrhea, has a number of factors known to contribute to pathogenesis; however, a full understanding of these processes and their regulation has proven to be elusive. Post-translational modifications (PTMs) of bacterial proteins are now recognized as one mechanism of protein regulation. In the present study, Western blot analyses, with an anti-acetyl-lysine antibody, indicated that a large number of gonococcal proteins are post-translationally modified. Previous work has shown that Nε-lysine acetylation can occur non-enzymatically with acetyl-phosphate (AcP) as the acetyl donor...
2017: PloS One
https://www.readbyqxmd.com/read/28654627/effect-of-programmed-exercise-on-insulin-sensitivity-in-postmenopausal-women-a-systematic-review-and-meta-analysis-of-randomized-controlled-trials
#11
Juan Bueno-Notivol, Julia Calvo-Latorre, Vanesa Alonso-Ventura, Vinay Pasupuleti, Adrian V Hernandez, Faustino R Pérez-López
OBJECTIVE: We performed a systematic review and meta-analysis of randomized controlled trials (RCTs) assessing the effect of programmed exercise for at least 12 weeks, in postmenopausal women on insulin sensitivity-related outcomes (ISROs), including fasting insulin, C-peptide, insulin growth factor (IGF-1) and IGF-binding protein (IGFBP-3), Homeostatic Model Assessment-Insulin Resistance (HOMA-IR), and anthropometric variables. METHODS: Searches were conducted in PubMed-Medline, Embase, Scopus, Web of Science, and Cochrane Library from inception through May 3, 2016, for studies published in all languages...
June 26, 2017: Menopause: the Journal of the North American Menopause Society
https://www.readbyqxmd.com/read/28654386/dual-energy-spect-and-the-development-of-peptide-p5-14-for-imaging-amyloidosis
#12
Jonathan S Wall, Stephen J Kennel, Emily B Martin
Amyloidosis is associated with a number of rare diseases and is characterized by the deposition, in abdominothoracic organs and peripheral nerves, of extracellular protein fibrils, which leads to dysfunction and severe morbidity. Effective clinical evaluation and management of patients with systemic amyloidosis are hampered by the lack of a noninvasive, quantitative method for detecting whole-body amyloid load. We have used a battery of assays including dual-energy SPECT imaging and comparative effectiveness studies in support of translation of a synthetic polybasic peptide, p5+14, as a novel radiotracer for visualization of amyloidosis by molecular imaging...
January 1, 2017: Molecular Imaging
https://www.readbyqxmd.com/read/28654282/the-reduction-in-the-ige-binding-ability-of-%C3%AE-lactoglobulin-by-dynamic-high-pressure-microfluidization-coupled-with-glycation-treatment-revealed-by-high-resolution-mass-spectrometry
#13
Yuan Chen, Zongcai Tu, Hui Wang, Qiuting Zhang, Lu Zhang, Xiaomei Sha, Tao Huang, Da Ma, Juanjuan Pang, Ping Yang
Our previous study indicated that pretreatment by dynamic high pressure microfluidization (DHPM) and glycation with galactose was a promising method for decreasing the immunoglobulin E (IgE) binding ability of β-lactoglobulin (β-LG). In present work, the conformational alteration of β-LG subjected to DHPM and glycation treatment were investigated in relation to IgE binding ability by Orbitrap mass spectrometry. After DHPM pretreatment, lower IgE binding ability of glycated β-LG was observed with increasing pressures...
June 27, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28654272/branched-amphipathic-peptide-capsules-different-ratios-of-the-two-constituent-peptides-direct-distinct-bilayer-structures-sizes-and-dna-transfection-efficiency
#14
Sheila de Mello Barros, L Adriana Avila, Susan K Whitaker, Kayla E Wilkinson, Pinakin Sukthankar, Eduardo Isidoro Carneiro Beltrão, John M Tomich
Branched Amphipathic Peptide Capsules (BAPCs) are biologically derived, bilayer delimited, nano-vesicles capable of being coated by or encapsulating a wide variety of solutes. The vesicles and their cargos are readily taken up by cells and become localized in the peri-nuclear region of cells. When BAPCs are mixed with DNA, the BAPCs act as cationic nucleation centers around which DNA winds. The BAPCs-DNA nanoparticles are capable of delivering plasmid DNA in vivo and in vitro yielding high transfection rates and minimal cytotoxicity...
June 27, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28654243/mitochondria-targeted-triphenylphosphonium-based-compounds-syntheses-mechanisms-of-action-and-therapeutic-and-diagnostic-applications
#15
Jacek Zielonka, Joy Joseph, Adam Sikora, Micael Hardy, Olivier Ouari, Jeannette Vasquez-Vivar, Gang Cheng, Marcos Lopez, Balaraman Kalyanaraman
Mitochondria are recognized as one of the most important targets for new drug design in cancer, cardiovascular, and neurological diseases. Currently, the most effective way to deliver drugs specifically to mitochondria is by covalent linking a lipophilic cation such as an alkyltriphenylphosphonium moiety to a pharmacophore of interest. Other delocalized lipophilic cations, such as rhodamine, natural and synthetic mitochondria-targeting peptides, and nanoparticle vehicles, have also been used for mitochondrial delivery of small molecules...
June 27, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28654112/distinguishing-d-and-l-aspartic-and-isoaspartic-acids-in-amyloid-%C3%AE-peptides-with-ultrahigh-resolution-ion-mobility-spectrometry
#16
Xueyun Zheng, Liulin Deng, Erin S Baker, Yehia M Ibrahim, Vladislav A Petyuk, Richard D Smith
While α-linked amino acids in the l-form are exclusively utilized in mammalian protein building, β-linked and d-form amino acids also have important biological roles. Unfortunately, the structural elucidation and separation of these different amino acid types in peptides has been analytically challenging to date due to the numerous isomers present, limiting our knowledge about their existence and biological roles. Here, we utilized an ultrahigh resolution ion mobility spectrometry platform coupled with mass spectrometry (IMS-MS) to separate amyloid β (Aβ) peptides containing l-aspartic acid, d-aspartic acid, l-isoaspartic acid, and d-isoaspartic acid residues which span α- and β-linked amino acids in both d- and l-forms...
June 27, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28654021/vitamin-c-aging-and-alzheimer-s-disease
#17
REVIEW
Fiammetta Monacelli, Erica Acquarone, Chiara Giannotti, Roberta Borghi, Alessio Nencioni
Accumulating evidence in mice models of accelerated senescence indicates a rescuing role of ascorbic acid in premature aging. Supplementation of ascorbic acid appeared to halt cell growth, oxidative stress, telomere attrition, disorganization of chromatin, and excessive secretion of inflammatory factors, and extend lifespan. Interestingly, ascorbic acid (AA) was also found to positively modulate inflamm-aging and immunosenescence, two hallmarks of biological aging. Moreover, ascorbic acid has been shown to epigenetically regulate genome integrity and stability, indicating a key role of targeted nutrition in healthy aging...
June 27, 2017: Nutrients
https://www.readbyqxmd.com/read/28654001/profiling-the-extended-cleavage-specificity-of-the-house-dust-mite-protease-allergens-der-p-1-der-p-3-and-der-p-6-for-the-prediction-of-new-cell-surface-protein-substrates
#18
Alain Jacquet, Vincenzo Campisi, Martyna Szpakowska, Marie-Eve Dumez, Moreno Galleni, Andy Chevigné
House dust mite (HDM) protease allergens, through cleavages of critical surface proteins, drastically influence the initiation of the Th2 type immune responses. However, few human protein substrates for HDM proteases have been identified so far, mainly by applying time-consuming target-specific individual studies. Therefore, the identification of substrate repertoires for HDM proteases would represent an unprecedented key step toward a better understanding of the mechanism of HDM allergic response. In this study, phage display screenings using totally or partially randomized nonameric peptide substrate libraries were performed to characterize the extended substrate specificities (P₅-P₄') of the HDM proteases Der p 1, Der p 3 and Der p 6...
June 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28653834/residue-specific-peptide-modification-a-chemist-s-guide
#19
Justine Nicole deGruyter, Lara R Malins, Phil S Baran
Advances in bioconjugation and native protein modification are appearing at a blistering pace, making it increasingly time consuming for practitioners to identify the best chemical method to modify a specific amino acid residue in a complex setting. The purpose of this perspective is to provide an informative, graphically rich manual highlighting significant advances in the field over the past decade. This guide will help triage candidate methods for peptide alteration, and will serve as a starting point for those seeking to solve longstanding challenges...
June 27, 2017: Biochemistry
https://www.readbyqxmd.com/read/28653830/photocontrollable-probe-spatiotemporally-induces-neurotoxic-fibrillar-aggregates-and-impairs-nucleocytoplasmic-trafficking
#20
Ruei-Yu He, Shu-Han Chao, Yu-Ju Tsai, Chi-Chang Lee, Chu-Yi Yu, Hua-De Gao, Yung-An Huang, Eric Hwang, Hsien-Ming Lee, Joseph Jen-Tse Huang
The abnormal assembly of misfolded proteins into neurotoxic aggregates is the hallmark associated with neurodegenerative diseases. Herein, we establish a photocontrollable platform to trigger amyloidogenesis to recapitulate the pathogenesis of amyotrophic lateral sclerosis (ALS) by applying a chemically engineered probe as a "switch" in live cells. This probe is composed of an amyloidogenic peptide from TDP-43, a photolabile linker, a polycationic sequence both to mask amyloidogenicity and for cell penetration, and a fluorophore for visualization...
June 27, 2017: ACS Nano
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