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https://www.readbyqxmd.com/read/29031228/peptide-based-electrochemical-biosensor-for-juvenile-idiopathic-arthritis-detection
#1
V R Rodovalho, G R Araujo, E R Vaz, C Ueira-Vieira, L R Goulart, J M Madurro, A G Brito-Madurro
Juvenile idiopathic arthritis (JIA) is a wide group of diseases, characterized by synovial inflammation and joint tissue damage. Due to the delay in the implementation of biomarkers into clinical practice and the association with severe sequels, there is an imperative need for new JIA diagnosis strategies. Electrochemical biosensors based on screen-printed electrodes and peptides are promising alternatives for molecular diagnosis. In this work, a novel biosensor for detecting juvenile idiopathic arthritis (JIA) was developed based on the immobilization of the PRF+1 mimetic peptide, as recognition biological element, on the surface of screen-printed carbon electrode...
October 5, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29031128/electrochemically-mediated-in-situ-growth-of-electroactive-polymers-for-highly-sensitive-detection-of-double-stranded-dna-without-sequence-preference
#2
Qiong Hu, Qiangwei Wang, Jinming Kong, Lianzhi Li, Xueji Zhang
The ability to directly detect double-stranded DNA (dsDNA) without sequence-preference continues to be a major challenge. Herein, we report an electrochemical method for the direct, highly sensitive detection of dsDNA based on the strand replacement of dsDNA by peptide nucleic acid (PNA) and the in situ growth of electroactive polymers through the surface-initiated electrochemically mediated atom transfer radical polymerization (SI-eATRP). Thiolated PNA molecules are firstly self-assembled onto gold electrode surface for the specific recognition of target dsDNA (dsDNA-T), which in turn leads to the formation of a high density of PNA/DNA heteroduplexes on the electrode surface for the subsequent attachment of ATRP initiators via the phosphate-Zr(4+)-carboxylate chemistry...
September 28, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29031069/synthesis-and-biological-evaluation-of-a-platinum-ii-c-rgdyk-conjugate-for-integrin-targeted-photodynamic-therapy
#3
T Chatzisideri, S Thysiadis, S Katsamakas, P Dalezis, I Sigala, T Lazarides, E Nikolakaki, D Trafalis, O A Gederaas, M Lindgren, V Sarli
A cancer-targeting conjugate 4 of a cyclometalated [N,C,N-Pt(II)] complex bearing a NˆCˆN 1,3-di(2-pyridyl)-benzene with c(RGDyK) peptide as guiding molecule was designed and synthesized for real-time drug delivery monitoring in cancer cells and photodynamic therapy (PDT). This conjugate demonstrates a mild cytostatic effect to six cancer cell lines expressing integrins at different extent, while possessing promising features for PDT. Conjugate 4 demonstrated rapid cell uptake by receptor-mediated endocytosis and efficient generation of (1)O2 upon irradiation...
September 28, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29029327/the-exonization-and-functionalization-of-an-alu-j-element-in-the-protein-coding-region-of-glycoprotein-hormone-alpha-gene-represent-a-novel-mechanism-to-the-evolution-of-hemochorial-placentation-in-primates
#4
Haidi Chen, Li Chen, Yune Wu, Hao Shen, Guang Yang, Cheng Deng
Alu elements contribute considerably to gene regulation and genome evolution in primates. The generation of new exons from Alu elements has been found in various human genes, and the regulatory function of the Alu exon has been investigated in many studies. However, the functionalization of Alu elements in protein coding regions remains unknown. Here, we reported that an Alu-J element exonized in the glycoprotein hormone alpha (GPHA) gene and encoded an additional N-terminal peptide (Alu-J encoding peptide) of the mature GPHA peptide, leading to a splicing variant of Alu-GPHA in anthropoid primates approximately 35 million years ago...
September 26, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/29029265/preclinical-testing-of-a-broad-spectrum-antimicrobial-endotracheal-tube-coated-with-an-innate-immune-synthetic-mimic
#5
Marjan M Hashemi, John Rovig, Jordan Bateman, Brett S Holden, Tomasz Modelzelewski, Iliana Gueorguieva, Martin von Dyck, Ronald Bracken, Carl Genberg, Shenglou Deng, Paul B Savage
Background: Endotracheal tubes provide an abiotic surface on which bacteria and fungi form biofilms, and the release of endotoxins and planktonic organisms can cause damaging inflammation and infections. Objectives: Ceragenins are small molecule mimics of antimicrobial peptides with broad-spectrum antibacterial and antifungal activity, and a ceragenin may be used to provide antimicrobial protection to the abiotic surface of an endotracheal tube. Methods: A hydrogel film, containing CSA-131, was generated on endotracheal tubes...
October 4, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29028926/structure-based-prediction-of-protein-peptide-binding-regions-using-random-forest
#6
Ghazaleh Taherzadeh, Yaoqi Zhou, Alan Wee-Chung Liew, Yuedong Yang
Motivation: Protein-peptide interactions are one of the most important biological interactions and play crucial role in many diseases including cancer. Therefore, knowledge of these interactions provides invaluable insights into all cellular processes, functional mechanisms, and drug discovery. Protein-peptide interactions can be analyzed by studying the structures of protein-peptide complexes. However, only a small portion has known complex structures and experimental determination of protein-peptide interaction is costly and inefficient...
September 26, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29028897/sipros-ensemble-improves-database-searching-and-filtering-for-complex-metaproteomics
#7
Xuan Guo, Zhou Li, Qiuming Yao, Ryan S Mueller, Jimmy K Eng, David L Tabb, William Judson Hervey, Chongle Pan
Motivation: Complex microbial communities can be characterized by metagenomics and metaproteomics. However, metagenome assemblies often generate enormous, and yet incomplete, protein databases, which undermines the identification of peptides and proteins in metaproteomics. This challenge calls for increased discrimination of true identifications from false identifications by database searching and filtering algorithms in metaproteomics. Results: Sipros Ensemble was developed here for metaproteomics using an ensemble approach...
September 22, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29028798/obstructive-sleep-apnea-and-rhonchopathy-are-associated-with-downregulation-of-trefoil-factor-family-peptide-3-tff3-implications-of-changes-in-oral-mucus-composition
#8
Regina Siber-Hoogeboom, Martin Schicht, Sebastian Hoogeboom, Friedrich Paulsen, Maximilian Traxdorf
STUDY OBJECTIVES: Trefoil factor family (TFF) peptides belong to the family of mucin-associated peptides and are expressed in most mucosal surfaces. TFF peptides carry out functions such as proliferation and migration enhancement, anti-apoptosis, and wound healing. Moreover, TFFs are associated with mucins and interact with them as "linker peptides", thereby influencing mucus viscosity. To test the hypothesis that in rhonchopathy and obstructive sleep apnea (OSA) changes occur in the expression of TFF3 and -2 that could contribute to changes in mucus viscosity, leading to an increase in upper airway resistance during breathing...
2017: PloS One
https://www.readbyqxmd.com/read/29028796/bak1-is-involved-in-atralf1-induced-inhibition-of-root-cell-expansion
#9
Keini Dressano, Paulo H O Ceciliato, Aparecida L Silva, Juan Carlos Guerrero-Abad, Tábata Bergonci, Fausto Andrés Ortiz-Morea, Marco Bürger, Marcio C Silva-Filho, Daniel S Moura
The rapid alkalinization factor (RALF) peptide negatively regulates cell expansion, and an antagonistic relationship has been demonstrated between AtRALF1, a root-specific RALF isoform in Arabidopsis, and brassinosteroids (BRs). An evaluation of the response of BR signaling mutants to AtRALF1 revealed that BRI1-associated receptor kinase1 (bak1) mutants are insensitive to AtRALF1 root growth inhibition activity. BAK1 was essential for the induction of AtRALF1-responsive genes but showed no effect on the mobilization of Ca2+ and alkalinization responses...
October 13, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29028607/ph-induced-interfacial-properties-of-chaplin-e-from-streptomyces-coelicolor
#10
Mina Dokouhaki, Andrew Hung, Emma L Prime, Greg G Qiao, Li Day, Sally L Gras
Chaplin E, or Chp E, is a surface active peptide secreted by Streptomyces coelicolor that adopts different structures depending on solution pH but the effect of these structures on the interfacial properties of Chp E is not known. In experiments paired with simulations, Chp E was found to display pH-dependent interfacial assembly and surface activity. At pH 3.0, Chp E formed an ordered non-amyloidal interfacial film with high surface activity; while at pH 10.0, Chp E self-assembled into a heterogeneous film containing randomly arranged fibrils at the interface that was less surface active compared to the film formed at pH 3...
October 4, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29028606/inflammatory-and-biocompatibility-evaluation-of-antimicrobial-peptide-gl13k-immobilized-onto-titanium-by-silanization
#11
Lin Zhou, Zhaonan Lin, Jiamin Ding, Wenxiu Huang, Jiang Chen, Dong Wu
The inflammatory reaction around the implant after implant placement is important not only for osseointegration but also for long-term implant survivals. In our study, GL13K, an antimicrobial peptide, was immobilized onto titanium surfaces to improve its anti-inflammatory properties. The method of silanization was used to immobilize the GL13K, which was confirmed by X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy, water contact angle measurement. DAPI fluorescence staining and Cell Counting Kit-8 (CCK-8) were used to measure the cell attachment and cell viability of the RAW264...
October 4, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29028529/design-synthesis-and-immunological-evaluation-of-novel-amphiphilic-desmuramyl-peptides
#12
Farooq-Ahmad Khan, Marina Ulanova, Bing Bai, Damayanthi Yalamati, Zi-Hua Jiang
Muramyl dipeptide (MDP) - an essential bacterial cell wall component - is recognized by our immune system as pathogen-associated molecular pattern (PAMP) which results in immune responses with adverse toxic effects. In order to harness the beneficial properties from the pro-inflammatory characteristics of the bacterial cell wall motif, MDP was strategically re-designed while conserving the L-D configurations of the dipeptide moiety. The muramic acid was replaced with a hydrophilic arene and lipophilic chain was introduced at peptide end to give the amphiphilic desmuramyl peptides (DMPs)...
September 30, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29028440/intracerebroventricular-infusion-of-an-angiotensin-at2-receptor-agonist-novokinin-aggravates-some-diabetes-mellitus-induced-alterations-in-wistar-rats
#13
Daniela Pechlivanova, Kiril Petrov, Petar Grozdanov, Zlatina Nenchovska, Jana Tchekalarova, Alexander Stoynev
Cumulative data suggest the significant role of the Renin - Angiotensin System in the development of the pathological consequences of diabetes mellitus (DM). Newly synthesized AT2 receptor agonists gained importance as a target for creating new antihypertensives. The aim of the present work was to study the effects of a peptide AT2 agonist Novokinin, infused intracerebroventricularly, on the consequences of the streptozotocin-induced type 1 DM (T1 DM) in Wistar rats. Food and water consumption, body weight, urine excretion (metabolic cages), motor activity (open field test), anxiety (elevated plus maze), nociception (paw pressure analgesimeter test), spatial memory (T-maze alternation test) and plasma levels of glucose and corticosterone (ELISA) were assessed two weeks after the T1 DM induction...
October 13, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/29028405/evaluation-of-plasma-peptides-extraction-methods-by-high-resolution-mass-spectrometry
#14
N V Zakharova, A Y Shornikova, A E Bugrova, V V Baybakova, M I Indeykina, A S Kononikhin, I A Popov, O I Kechko, A A Makarov, E N Nikolaev
Monitoring of peptides offers a promising approach for the discovery of novel biomarkers, which might be valuable for detection, treatment and prevention of large variety of diseases. Development of highly effective methods for plasma peptide extraction remains an important task. In the current study, we applied different types of plasma peptide extraction approaches to reveal efficient methods which would provide the highest peptide yield. We used different combinations of plasma treatment with acetonitrile and/or urea/guanidine, protein precipitation by acetone, gel-filtration, ultrafiltration, and two types of solid phase extraction...
August 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29028400/label-free-study-of-cosmonaut-s-urinary-proteome-changes-after-long-duration-spaceflights
#15
A Brzhozovskiy, A Kononikhin, M Indeykina, LKh Pastushkova, I A Popov, E N Nikolaev, I M Larina
During the entire time that cosmonauts stay on board the international space station, different extreme space flight factors affect their bodies. In order to find out what physiological changes occur under the influence of spaceflight, different parameters of the human body before and after flights are monitored. Analysis of the urine proteome is one of the most perspective non-invasive methods of condition monitoring. The aim of the study was to perform a comparative semi-quantitative label-free urine proteome analysis of samples collected from 21 cosmonauts before and after long-duration spaceflight at the international space station...
August 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29028392/application-of-selected-reaction-monitoring-and-parallel-reaction-monitoring-for-investigation-of-hl-60-cell-line-differentiation
#16
Svetlana E Novikova, Olga V Tikhonova, Leonid K Kurbatov, Tatiana E Farafonova, Igor V Vakhrushev, Victor G Zgoda
Targeted mass spectrometry represents a powerful tool for investigation of biological processes. The convenient approach of selected reaction monitoring using stable isotope-labeled peptide standard (SIS) is widely applied for protein quantification. Along with this method, high-resolution parallel reaction monitoring has been increasingly used for protein targeted analysis. Here we applied two targeted approaches (selected reaction monitoring with SIS and label-free parallel reaction monitoring) to investigate expression of 11 proteins during all-trans retinoic acid-induced differentiation of HL-60 cells...
August 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29028384/effect-of-metal-surfaces-on-matrix-assisted-laser-desorption-ionization-analyte-peak-intensities
#17
Vidhyullatha Kancharla, Sajid Bashir, Jingbo L Liu, Oscar M Ramirez, Peter J Derrick, Kyle A Beran
Different metal surfaces in the form of transmission electron microscope grids were examined as support surfaces in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with a view towards enhancement of peptide signal intensity. The observed enhancement between 5-fold and 20-fold relative to the normal stainless steel slide was investigated by applying the thermal desorption model for matrix-assisted laser desorption/ionization. A simple model evaluates the impact that the thermal properties of the metals have on the ion yield of the analyte...
October 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29028351/a-hexamer-of-a-peptide-derived-from-a%C3%AE-16-36
#18
Adam G Kreutzer, Ryan K Spencer, Kate J McKnelly, Stan Yoo, Imane L Hamza, Patrick J Salveson, James S Nowick
The absence of high-resolution structures of amyloid oligomers constitutes a major gap in our understanding of amyloid diseases. Increasing evidence indicates that oligomers of the β-amyloid peptide Aβ are especially important in the progression of Alzheimer's disease. In many Aβ oligomers, the Aβ monomer components are thought to adopt a β-hairpin conformation. This paper describes the design and study of a macrocyclic β-hairpin peptide derived from Aβ16-36. SDS-PAGE and size exclusion chromatography studies show that the Aβ16-36 β-hairpin peptide assembles in solution to form hexamers, trimers, and dimers...
October 13, 2017: Biochemistry
https://www.readbyqxmd.com/read/29028315/nanoparticles-of-short-cationic-peptidopolysaccharide-self-assembled-by-hydrogen-bonding-with-antibacterial-effect-against-multi-drug-resistant-bacteria
#19
Zheng Hou, Yogesh Vikhe Shankar, Yang Liu, Feiqing Ding, Jothy Lachumy Subramanion, Vikashini Ravikumar, Rubí Zamudio-Vázquez, Damien Keogh, Huiwen Lim, Moon Yue Feng Tay, Surajit Bhattacharjya, Scott A Rice, Jian Shi, Hongwei Duan, Xue-Wei Liu, Yuguang Mu, Nguan Soon Tan, Kam Michael Chiu Tam, Kevin Pethe, Mary B Chan-Park
Cationic antimicrobial peptides (AMPs) and polymers are active against many multi-drug resistant (MDR) bacteria but only a limited number of these compounds are in clinical use due to their unselective toxicity. The typical strategy for achieving selective antibacterial efficacy with low mammalian cell toxicity is through balancing the ratio of cationicity to hydrophobicity. Herein, we report a cationic nanoparticle self-assembled from chitosan-graft-oligolysine (CSM5-K5) chains with ultra-low molecular weight (1450 Daltons) that selectively kills bacteria...
October 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29028283/implementation-of-buffy-coat-derived-pooled-platelet-concentrates-for-internal-quality-control-of-light-transmission-aggregometry-a-proof-of-concept-study
#20
F Prüller, K Rosskopf, H Mangge, E Mahla, D von Lewinski, E C Weiss, A Riegler, D Enko
BACKGROUND: Standardized commercially available control material for internal quality control (IQC) of light transmission aggregometry (LTA) is still lacking. Moreover, the provision of normal blood donors to provide fresh platelets is difficult in small laboratories, where "volunteers" may be of short supply. OBJECTIVES: To evaluate the implementation of buffy-coat-derived pooled platelet concentrates (PCs) for IQC material of LTA. METHODS: We used buffy-coat-derived pooled PCs from the blood bank as IQC material for LTA...
October 13, 2017: Journal of Thrombosis and Haemostasis: JTH
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