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https://www.readbyqxmd.com/read/29017849/production-of-hiv-virus-like-particles-by-transient-transfection-of-cap-t-cells-at-bioreactor-scale-avoiding-medium-replacement
#1
Sonia Gutiérrez-Granados, Queralt Farràs, Kerstin Hein, Javier Fuenmayor, Pablo Félez, Mercedes Segura, Francesc Gòdia
Human-derived CAP-T cell line has been demonstrated to be a powerful platform for high-titer production of HIV virus-like particles (VLPs) by PEI-mediated transient transfection. Scale-up of transfection processes is key to ensure the necessary quantities for pre-clinical and clinical testing. One of the major operational challenges of large-scale transient transfection is the medium replacement step that is often required before transfection. In this work, CAP-T cells were cultured in 1L bioreactor with addition of sodium bicarbonate and surface aeration, which were observed to improve cell state for transfection...
October 7, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28976765/growth-factor-releasing-polyelectrolyte-multilayer-films-to-control-the-cell-culture-environment
#2
Ivan Ding, Dalia M Shendi, Marsha W Rolle, Amy M Peterson
Polyelectrolyte multilayers (PEMs) are of great interest as cell culture surfaces because of their ability to modify topography and surface energy and release biologically relevant molecules such as growth factors. In this work, fibroblast growth factor 2 (FGF2) was adsorbed directly onto polystyrene, plasma-treated polystyrene, and glass surfaces with a poly(methacrylic acid) and poly-l-histidine PEM assembled above it. Up to 14 ng/cm(2) of FGF2 could be released from plasma-treated polystyrene surfaces over the course of 7 days with an FGF2 solution concentration of 100 μg/mL applied during the adsorption process...
October 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28976532/interfacial-and-structural-characteristics-of-polyelectrolyte-multilayers-used-as-cushions-for-supported-lipid-bilayers
#3
M Wlodek, M Kolasinska-Sojka, M Wasilewska, O Bikondoa, W H Briscoe, P Warszynski
The surface properties of polyelectrolyte multilayers (PEMs) obtained via sequential adsorption of oppositely charged polyions from their solutions and used as cushions for supported lipid bilayers were investigated. Five types of polyelectrolytes were used: cationic polyethyleneimine (PEI), poly(diallyldimethylammonium)chloride (PDADMAC), and poly-l-lysine hydrobromide (PLL); and anionic polysodium 4-styrenesulfonate (PSS) and poly-l-glutamic acid sodium (PGA). The wettability and surface free energy of the PEMs were determined by contact angle measurements using sessile drop analysis...
October 4, 2017: Soft Matter
https://www.readbyqxmd.com/read/28968503/hexagonal-molecular-tiling-by-hexagonal-macrocycles-at-the-liquid-solid-interface-structural-effects-on-packing-geometry
#4
Kohei Iritani, Motoki Ikeda, Anna Yang, Kazukuni Tahara, Keiji Hirose, Jeffrey Moore, Yoshito Tobe
We present here hexagonal tiling using hexagonal phenylene-ethynylene and phenylene-butadiynylene macrocycles attached by alkyl ester groups, PEM-C6 and PBM-C8, respectively, or triethylene glycol ester groups, PEM-TEG and PBM-TEG, respectively, at each vertex of the macrocyclic periphery at the liquid/solid interface. In this study, we focused on the effects of macrocyclic core size and the chemical properties of side chains attached to macrocyclic cores as well as solute concentrations on hexagonal geometry of self-assembled monolayers...
October 2, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28964569/protein-blend-ingestion-before-allogeneic-stem-cell-transplantation-improves-protein-energy-malnutrition-in-patients-with-leukemia
#5
Guangxu Ren, Jianping Zhang, Minghua Li, Suqin Yi, Jin Xie, Hongru Zhang, Jing Wang
Severe protein-energy malnutrition (PEM) and skeletal muscle wasting are commonly observed in patients with acute leukemia. Recently, the ingestion of a soy-whey protein blend has been shown to promote muscle protein synthesis (MPS). Thus, we tested the hypothesis that the ingestion of a soy-whey blended protein (BP) may improve the PEM status and muscle mass in acute leukemia patients. In total, 24 patients from the same treatment group were randomly assigned to the natural diet plus soy-whey blended protein (BP) group and the natural diet only (ND) group...
August 25, 2017: Nutrition Research
https://www.readbyqxmd.com/read/28963772/bacterial-cellulose-skin-masks-properties-and-sensory-tests
#6
Guilherme Pacheco, Carolina Véspoli de Mello, Bruna Galdorfini Chiari-Andréo, Vera Lucia Borges Isaac, Sidney José Lima Ribeiro, Édison Pecoraro, Eliane Trovatti
BACKGROUND: Bacterial cellulose (BC) is a versatile material produced by microorganisms in the form of a membranous hydrogel, totally biocompatible, and endowed with high mechanical strength. Its high water-holding capacity based on its highly porous nanofibrillar structure allows BC to incorporate and to release substances very fast, thus being suitable for the preparation of skincare masks. AIMS: The preparation and characterization of cosmetic masks based on BC membranes and active cosmetics...
September 29, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28961118/advanced-boundary-electrode-modeling-for-tes-and-parallel-tes-eeg
#7
S Pursiainen, B Agsten, S Wagner, C H Wolters
This paper explores advanced electrode modeling in the context of separate and parallel transcranial electrical stimulation (tES) and electroencephalography (EEG) measurements.We focus on boundary condition based approaches that do not necessitate adding auxiliary elements, e.g. sponges, to the computational domain. In particular, we investigate the complete electrode model (CEM) which incorporates a detailed description of the skin-electrode interface including its contact surface, impedance and normal current distribution...
September 28, 2017: IEEE Transactions on Neural Systems and Rehabilitation Engineering
https://www.readbyqxmd.com/read/28952721/two-dimensional-zeolitic-imidazolate-framework-carbon-nanotube-hybrid-networks-modified-proton-exchange-membranes-for-improving-transport-properties
#8
Huazhen Sun, Beibei Tang, Peiyi Wu
Metal-organic framework (MOF)/polymer composite proton exchange membranes (PEMs) are being intensively investigated due to their potentials for the systematic design of proton-conducting properties. However, the development of MOF/polymer composite PEMs possessing high selectivity remains exceedingly desirable and challenging for practical application. Herein, two-dimensional (2D) zeolitic imidazolate framework (ZIF-8)/carbon nanotube (CNT) hybrid cross-linked networks (ZCN) were synthesized via the rational design of the physical form of ZIF-8, and then a series of composite PEMs were prepared by hybridizing ZCN with sulfonated poly(ether ether ketone) (SPEEK) matrix...
October 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28946792/calvarial-bone-regeneration-is-enhanced-by-sequential-delivery-of-fgf-2-and-bmp-2-from-layer-by-layer-coatings-with-a-biomimetic-calcium-phosphate-barrier-layer
#9
Gloria Gronowicz, Emily Jacobs, Tao Peng, Li Zhu, Marja Hurley, Liisa T Kuhn
A drug delivery coating for synthetic bone grafts has been developed to provide sequential delivery of multiple osteoinductive factors to better mimic aspects of the natural regenerative process. The coating is composed of a biomimetic calcium phosphate (bCaP) layer that is applied to a synthetic bone graft and then covered with a poly-L-Lysine/poly-L-Glutamic acid polyelectrolyte multilayer (PEM) film. Bone morphogenetic protein-2 (BMP-2) was applied prior to the coating process directly on the synthetic bone graft and then bCaP-PEM was deposited followed by adsorption of fibroblast growth factor-2 (FGF-2) into the PEM layer...
September 25, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28944504/creating-and-sustaining-an-academic-practice-partnership-engagement-model
#10
Marjorie A Schaffer, Patricia M Schoon, Bonnie L Brueshoff
Public health clinical educators and practicing public health nurses (PHNs) are experiencing challenges in creating meaningful clinical learning experiences for nursing students due to an increase in nursing programs and greater workload responsibilities for both nursing faculty and PHNs. The Henry Street Consortium (HSC), a collaborative group of PHNs and nursing faculty, conducted a project to identify best practices for public health nursing student clinical learning experiences. Project leaders surveyed HSC members about preferences for teaching-learning strategies, facilitated development of resources and tools to guide learning, organized faculty/PHN pilot teams to test resources and tools with students, and evaluated the pilot team experiences through two focus groups...
September 25, 2017: Public Health Nursing
https://www.readbyqxmd.com/read/28940903/therapeutic-vesicular-nanoreactors-with-tumor-specific-activation-and-self-destruction-for-synergistic-tumor-ablation
#11
Junjie Li, Anjaneyulu Dirisala, Zhishen Ge, Yuheng Wang, Wei Yin, Wendong Ke, Kazuko Toh, Jinbing Xie, Yu Matsumoto, Yasutaka Anraku, Kensuke Osada, Kazunori Kataoka
Polymeric nanoreactors (NRs) have distinct advantages to improve chemical reaction efficiency, but the in vivo applications are limited by lack of tissue-specificity. Herein, novel glucose oxidase (GOD)-loaded therapeutic vesicular NRs (theraNR) are constructed based on a diblock copolymer containing poly(ethylene glycol) (PEG) and copolymerized phenylboronic ester or piperidine-functionalized methacrylate (P(PBEM-co-PEM)). Upon systemic injection, theraNR are inactive in normal tissues. At a tumor site, theraNR are specifically activated by the tumor acidity via improved permeability of the membranes...
September 20, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28934453/an-adjuvanted-a-h5n1-subvirion-vaccine-elicits-virus-specific-antibody-response-and-improves-protection-against-lethal-influenza-viral-challenge-in-mouse-model-of-protein-energy-malnutrition
#12
Enitra N Jones, Samuel Amoah, Weiping Cao, Suryaprakash Sambhara, Shivaprakash Gangappa
Background: Protein energy malnutrition (PEM) increases susceptibility to infectious diseases, including influenza infection, but no studies have addressed the potential influences of PEM on the immunogenicity and protective efficacy of avian influenza A(H5N1) vaccine. Methods: We investigated the role of PEM on vaccine-mediated protection after a lethal challenge with recombinant A(H5N1) virus using isocaloric diets providing either adequate protein (AP; 18% protein) or very low protein (VLP; 2% protein) in an established murine model of influenza vaccination...
September 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28915817/cultural-adaptation-of-a-pediatric-functional-assessment-for-rehabilitation-outcomes-research
#13
Kristen E Arestad, David MacPhee, Chun Y Lim, Mary A Khetani
BACKGROUND: Significant racial and ethnic health care disparities experienced by Hispanic children with special health care needs (CSHCN) create barriers to enacting culturally competent rehabilitation services. One way to minimize the impact of disparities in rehabilitation is to equip practitioners with culturally relevant functional assessments to accurately determine service needs. Current approaches to culturally adapting assessments have three major limitations: use of inconsistent translation processes; current processes assess for some, but not all, elements of cultural equivalence; and limited evidence to guide decision making about whether to undertake cultural adaptation with and without language translation...
September 15, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28905177/readability-assessment-of-online-patient-education-materials-provided-by-the-european-association-of-urology
#14
Patrick Betschart, Valentin Zumstein, Maico Bentivoglio, Daniel Engeler, Hans-Peter Schmid, Dominik Abt
PURPOSE: To assess the readability of the web-based patient education material provided by the European Association of Urology. MATERIALS AND METHODS: English patient education materials (PEM) as available in May 2017 were obtained from the EAU website. Each topic was analyzed separately using six well-established readability assessment tools, including Flesch-Kincaid Grade Level (FKGL), SMOG Grade Level (SMOG), Coleman-Liau Index (CLI), Gunning Fog Index (GFI), Flesch Reading Ease Formula (FRE) and Fry Readability Graph (FRG)...
September 13, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28901493/high-pemetrexed-sensitivity-of-docetaxel-resistant-a549-cells-is-mediated-by-tp53-status-and-downregulated-thymidylate-synthase
#15
Wei-Ting Kuo, Dom-Gene Tu, Ling-Yen Chiu, Gwo-Tarng Sheu, Ming-Fang Wu
The chemoresistance of non-small cell lung cancer (NSCLC) that occurs in docetaxel (DOC) chemotherapy substantially decreases the survival of patients. To overcome DOC-induced chemoresistance, we established DOC-selected A549 lung cancer sublines (A549/D16 and A549/D32) and revealed that both sublines were cross-resistant to vincristine (VCR) and doxorubicin (DXR). Notably, both sublines were more sensitive to pemetrexed (PEM) than parental cells according to MTT and clonogenic assays. The expression levels of thymidylate synthase (TS) and γ-glutamyl hydrolase (GGH) were downregulated in DOC-resistant sublines...
September 7, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28873304/multifunctional-bacteria-driven-microswimmers-for-targeted-active-drug-delivery
#16
Byung-Wook Park, Jiang Zhuang, Oncay Yasa, Metin Sitti
High-performance, multifunctional bacteria-driven microswimmers are introduced using an optimized design and fabrication method for targeted drug delivery applications. These microswimmers are made of mostly single Escherichia coli bacterium attached to the surface of drug-loaded polyelectrolyte multilayer (PEM) microparticles with embedded magnetic nanoparticles. The PEM drug carriers are 1 μm in diameter and are intentionally fabricated with a more viscoelastic material than the particles previously studied in the literature...
September 26, 2017: ACS Nano
https://www.readbyqxmd.com/read/28872939/psychometric-evaluation-of-the-young-children-s-participation-and-environment-measure-yc-pem-for-use-in-singapore
#17
Chun Yi Lim, Mary Law, Mary Khetani, Peter Rosenbaum, Nancy Pollock
AIMS: To estimate the psychometric properties of a culturally adapted version of the Young Children's Participation and Environment Measure (YC-PEM) for use among Singaporean families. METHODS: This is a prospective cohort study. Caregivers of 151 Singaporean children with (n = 83) and without (n = 68) developmental disabilities, between 0 and 7 years, completed the YC-PEM (Singapore) questionnaire with 3 participation scales (frequency, involvement, and change desired) and 1 environment scale for three settings: home, childcare/preschool, and community...
September 5, 2017: Physical & Occupational Therapy in Pediatrics
https://www.readbyqxmd.com/read/28872884/facile-synthesis-of-catalytic-aupd-nanoparticles-within-capillary-microreactors-using-polyelectrolyte-multilayers-for-the-direct-synthesis-of-h2o2
#18
Shamayita Kanungo, Violeta Paunovic, Jaap C Schouten, M Fernanda Neira D'Angelo
Microreactors present innovative solutions for problems pertaining to conventional reactors and therefore have seen successful application in several industrial processes. Yet, its application in heterogeneously catalyzed gas-liquid reactions has been challenging, mainly due to the lack of an easy and flexible methodology for catalyst incorporation inside these reactors. Herein, we report a facile technique for obtaining small (<2 nm) and well-distributed catalytic nanoparticles on the walls of silica-coated capillaries, that act as micro(channel) reactors...
October 11, 2017: Nano Letters
https://www.readbyqxmd.com/read/28872326/was-it-race-or-merit-the-cognitive-costs-of-observing-the-attributionally-ambiguous-hiring-of-a-racial-minority
#19
Kathy Espino-Pérez, Brenda Major, Brenna Malta
OBJECTIVES: This study investigated individual and situational factors that may make observing positive treatment of an ingroup member attributionally ambiguous and cognitively taxing for ethnic minority perceivers. METHOD: 163 Latino/a participants who varied in the perception that Whites are externally motivated to behave positively toward minorities (Perceived External Motivation Scale; PEMS) observed a Latino candidate selected over 2 White candidates by a White Human Resources officer...
September 4, 2017: Cultural Diversity & Ethnic Minority Psychology
https://www.readbyqxmd.com/read/28866215/enhanced-antiadhesive-properties-of-chitosan-hyaluronic-acid-polyelectrolyte-multilayers-driven-by-thermal-annealing-low-adherence-for-mammalian-cells-and-selective-decrease-in-adhesion-for-gram-positive-bacteria
#20
Nicolás E Muzzio, Miguel A Pasquale, Eleftheria Diamanti, Danijela Gregurec, Marta Martinez Moro, Omar Azzaroni, Sergio E Moya
The development of antifouling coatings with restricted cell and bacteria adherence is fundamental for many biomedical applications. A strategy for the fabrication of antifouling coatings based on the layer-by-layer assembly and thermal annealing is presented. Polyelectrolyte multilayers (PEMs) assembled from chitosan and hyaluronic acid were thermally annealed in an oven at 37°C for 72h. The effect of annealing on the PEM properties and topography was studied by atomic force microscopy, ζ-potential, circular dichroism and contact angle measurements...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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