keyword
https://read.qxmd.com/read/38432774/genotoxicity-of-ultraviolet-light-and-sunlight-in-the-bacterium-caulobacter-crescentus-wavelength-dependence
#21
JOURNAL ARTICLE
Fabiana Fuentes-León, Nathalia Quintero-Ruiz, Frank S Fernández-Silva, Veridiana Munford, Marioly Vernhes Tamayo, Carlos Frederico Martins Menck, Rodrigo S Galhardo, Angel Sánchez-Lamar
The ultraviolet (UV) component of sunlight can damage DNA. Although most solar UV is absorbed by the ozone layer, wavelengths > 300 nm (UVA and UVB bands) can reach the Earth's surface. It is essential to understand the genotoxic effects of UV light, particularly in natural environments. Caulobacter crescentus, a bacterium widely employed as a model for cell cycle studies, was selected for this study. Strains proficient and deficient in DNA repair (uvrA-) were used to concurrently investigate three genotoxic endpoints: cytotoxicity, SOS induction, and gene mutation, using colony-formation, the SOS chromotest, and RifR mutagenesis, respectively...
2024: Mutation Research. Genetic Toxicology and Environmental Mutagenesis
https://read.qxmd.com/read/38424107/the-deinococcus-protease-ppri-senses-dna-damage-by-directly-interacting-with-single-stranded-dna
#22
JOURNAL ARTICLE
Huizhi Lu, Zijing Chen, Teng Xie, Shitong Zhong, Shasha Suo, Shuang Song, Liangyan Wang, Hong Xu, Bing Tian, Ye Zhao, Ruhong Zhou, Yuejin Hua
Bacteria have evolved various response systems to adapt to environmental stress. A protease-based derepression mechanism in response to DNA damage was characterized in Deinococcus, which is controlled by the specific cleavage of repressor DdrO by metallopeptidase PprI (also called IrrE). Despite the efforts to document the biochemical, physiological, and downstream regulation of PprI-DdrO, the upstream regulatory signal activating this system remains unclear. Here, we show that single-stranded DNA physically interacts with PprI protease, which enhances the PprI-DdrO interactions as well as the DdrO cleavage in a length-dependent manner both in vivo and in vitro...
February 29, 2024: Nature Communications
https://read.qxmd.com/read/38391790/assessing-the-structural-validity-of-the-knee-injury-and-osteoarthritis-outcome-score-scale
#23
JOURNAL ARTICLE
Dylan T Quintana, Madeline P Casanova, Adam C Cady, Russell T Baker
BACKGROUND: The Knee Injury and Osteoarthritis Outcome Score (KOOS) scale is used to assess patient perspectives on knee health. However, the structural validity of the KOOS has not been sufficiently tested; therefore, our objective was to assess the KOOS in a large, multi-site database of patient responses who were receiving care for knee pathology. METHODS: A cross-sectional study was conducted using the Surgical Outcome System (SOS) database. A confirmatory factor analysis (CFA) was conducted to assess the proposed five-factor KOOS using a priori cut-off values...
February 6, 2024: Healthcare (Basel, Switzerland)
https://read.qxmd.com/read/38379229/characters-of-the-moca-family-in-wheat-and-tamoca1-function-in-salt-stress-tolerance
#24
JOURNAL ARTICLE
Yuxiang Qin, Ping Cui, Bao Zhang, Yuning Wang
MOCA1 encodes the last key glucuronosyltransferase for ionic stress sensor glycosyl inositol phosphoryl-ceramide (GIPCs) biosynthesis in Arabidopsis , which indicates that the MOCA gene family play important role in plant tolerance to salt stress. However, the isolation and function of MOCAs in staple crops have not been reported and the downstream targets of MOCAs in salt stress tolerance signalling pathway are not clear. In this study, we identified 110 MOCA genes in wheat which were classified into five clades and they differed in gene structure, protein length, conserved motifs and expression profiles in different tissues and under salt stress...
2024: Journal of Genetics
https://read.qxmd.com/read/38377209/the-global-rna-rna-interactome-of-klebsiella-pneumoniae-unveils-a-small-rna-regulator-of-cell-division
#25
JOURNAL ARTICLE
Eric Ruhland, Malte Siemers, Ruman Gerst, Felix Späth, Laura Nicole Vogt, Marc Thilo Figge, Kai Papenfort, Kathrin Sophie Fröhlich
The ubiquitous RNA chaperone Hfq is involved in the regulation of key biological processes in many species across the bacterial kingdom. In the opportunistic human pathogen Klebsiella pneumoniae , deletion of the hfq gene affects the global transcriptome, virulence, and stress resistance; however, the ligands of the major RNA-binding protein in this species have remained elusive. In this study, we have combined transcriptomic, co-immunoprecipitation, and global RNA interactome analyses to compile an inventory of conserved and species-specific RNAs bound by Hfq and to monitor Hfq-mediated RNA-RNA interactions...
February 27, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38366016/protozoan-predation-enhances-stress-resistance-and-antibiotic-tolerance-in-burkholderia-cenocepacia-by-triggering-the-sos-response
#26
JOURNAL ARTICLE
Álvaro Morón, Alaa E Tarhouchi, Iván Belinchón, Juan M Valenzuela, Patricia de Francisco, Ana Martín-González, Francisco Amaro
Bacterivorous protists are thought to serve as training grounds for bacterial pathogens by subjecting them to the same hostile conditions that they will encounter in the human host. Bacteria that survive intracellular digestion exhibit enhanced virulence and stress resistance after successful passage through protozoa but the underlying mechanisms are unknown. Here we show that the opportunistic pathogen Burkholderia cenocepacia survives phagocytosis by ciliates found in domestic and hospital sink drains, and viable bacteria are expelled packaged in respirable membrane vesicles with enhanced resistance to oxidative stress, desiccation and antibiotics, thereby contributing to pathogen dissemination in the environment...
January 28, 2024: ISME Journal
https://read.qxmd.com/read/38365567/consideration-of-metastasis-directed-therapy-for-patients-with-metastatic-colorectal-cancer-expert-survey-and-systematic-review
#27
JOURNAL ARTICLE
Eric D Miller, Brett G Klamer, Jordan M Cloyd, Timothy M Pawlik, Terence M Williams, Kathryn E Hitchcock, Paul B Romesser, Harvey J Mamon, Kimmie Ng, Sepideh Gholami, George J Chang, Christopher J Anker
BACKGROUND: A survey of medical oncologists (MOs), radiation oncologists (ROs), and surgical oncologists (SOs) who are experts in the management of patients with metastatic colorectal cancer (mCRC) was conducted to identify factors used to consider metastasis-directed therapy (MDT). MATERIALS AND METHODS: An online survey to assess clinical factors when weighing MDT in patients with mCRC was developed based on systematic review of the literature and integrated with clinical vignettes...
January 30, 2024: Clinical Colorectal Cancer
https://read.qxmd.com/read/38340920/resilience-and-proteome-response-of-escherichia-coli-to-high-levels-of-isoleucine-mistranslation
#28
JOURNAL ARTICLE
Marija Pranjic, Philipp Spät, Maja Semanjski Curkovic, Boris Macek, Ita Gruic-Sovulj, Marko Mocibob
Accurate pairing of amino acids and tRNAs is a prerequisite for faithful translation of genetic information during protein biosynthesis. Here we present the effects of proteome-wide mistranslation of isoleucine (Ile) by canonical valine (Val) or non-proteinogenic norvaline (Nva) in a genetically engineered Escherichia coli strain with an editing-defective isoleucyl-tRNA synthetase (IleRS). Editing-defective IleRS efficiently mischarges both Val and Nva to tRNAIle and impairs the translational accuracy of Ile decoding...
February 8, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38289054/comparative-label-free-proteomics-of-the-neonatal-meningitis-causing-escherichia-coli-k1-ihe3034-and-rs218-morphotypes
#29
JOURNAL ARTICLE
Nikola Zlatkov, Wilma Gunnari, Ulrike Resch
The proteome of two newborn meningitis Escherichia coli K1 (NMEC) morphotypes was examined via a label-free proteomics approach. Besides shared NMEC virulence factors, the two strains have different evolutionary strategies-strain IHE3034 tends to perform anaerobic respiration continuously, while strain RS218 maintains its filamentous morphotype due to active SOS response.
January 30, 2024: Microbiology Resource Announcements
https://read.qxmd.com/read/38286262/performance-evaluation-for-the-inactivation-of-multidrug-resistant-bacteria-in-wastewater-effluent-by-different-disinfection-technologies
#30
JOURNAL ARTICLE
Yijing Shen, Jieling Luo, Andrea Di Cesare, Nairong Guo, Shichun Zou, Ying Yang
The escalating presence of antibiotic-resistant bacteria (ARB) in aquatic ecosystems underscores the critical role of wastewater treatment plants (WWTPs) in mitigating antibiotic resistance. Disinfection is the final, pivotal step in WWTPs, and it is essential to control the dissemination of ARB before water discharge. This study utilized both phenotypic analysis and transcriptome (RNA-seq) approach to investigate the efficiency and mechanisms of disinfection using chlorination, ultraviolet (UV), and peracetic acid (PAA) on multidrug-resistant bacteria (MRB)...
January 27, 2024: Environmental Pollution
https://read.qxmd.com/read/38245921/ultrasonic-cavitation-induced-vibrio-parahaemolyticus-entering-an-apoptosis-like-death-process-through-sos-response
#31
JOURNAL ARTICLE
Chunhui Liu, Qi Xu, Jiaqi Ma, Sai Wang, Jiao Li, Xiangzhao Mao
As an effective non-thermal sterilization method, ultrasound remains at the level of passive bacterial death despite the initial understanding of its sterilization mechanism. Here, we present the perspective that bacteria can choose to actively enter an apoptosis-like death state in response to external ultrasonic stress. In this study, Vibrio parahaemolyticus exhibited apoptotic markers such as phosphatidylserine ectropion and activated caspases when subjected to ultrasound stress. Additionally, the accumulation of reactive oxygen species (ROS) and enhanced calcium signaling were observed...
January 17, 2024: Ultrasonics Sonochemistry
https://read.qxmd.com/read/38244877/odor-cueing-of-declarative-memories-during-sleep-enhances-coordinated-spindles-and-slow-oscillations
#32
JOURNAL ARTICLE
Andrea Sánchez-Corzo, David M Baum, Martín Irani, Svenja Hinrichs, Renate Reisenegger, Grace A Whitaker, Jan Born, Ranganatha Sitaram, Jens G Klinzing
Long-term memories are formed by repeated reactivation of newly encoded information during sleep. This process can be enhanced by using memory-associated reminder cues like sounds and odors. While auditory cueing has been researched extensively, few electrophysiological studies have exploited the various benefits of olfactory cueing. We used high-density electroencephalography in an odor-cueing paradigm that was designed to isolate the neural responses specific to the cueing of declarative memories. We show widespread cueing-induced increases in the duration and rate of sleep spindles...
January 18, 2024: NeuroImage
https://read.qxmd.com/read/38224456/single-cell-evidence-for-plasmid-addiction-mediated-by-toxin-antitoxin-systems
#33
JOURNAL ARTICLE
Nathan Fraikin, Laurence Van Melderen
Toxin-antitoxin (TA) systems are small selfish genetic modules that increase vertical stability of their replicons. They have long been thought to stabilize plasmids by killing cells that fail to inherit a plasmid copy through a phenomenon called post-segregational killing (PSK) or addiction. While this model has been widely accepted, no direct observation of PSK was reported in the literature. Here, we devised a system that enables visualization of plasmid loss and PSK at the single-cell level using meganuclease-driven plasmid curing...
January 15, 2024: Nucleic Acids Research
https://read.qxmd.com/read/38205259/significant-early-end-of-the-growing-season-of-forest-vegetation-inside-china-s-protected-areas
#34
JOURNAL ARTICLE
Ya Liu, Jun Ma
The land surface phenology (LSP) indicators (i.e., start, end, and length of the growing season: SOS, EOS, LOS) are important to reflect the growth of forest and its response to environmental changes. However, the spatiotemporal variation and its mechanism of forest phenology under different human disturbance' levels are still unclear. Here, we compare the LSP indicators inside and outside China's 257 protected areas (PAs) and explore the influencing factors of phenological differences (ΔSOS, ΔEOS, ΔLOS)...
January 19, 2024: IScience
https://read.qxmd.com/read/38191636/stochastic-biological-system-of-systems-modelling-for-ipsc-culture
#35
JOURNAL ARTICLE
Hua Zheng, Sarah W Harcum, Jinxiang Pei, Wei Xie
Large-scale manufacturing of induced pluripotent stem cells (iPSCs) is essential for cell therapies and regenerative medicines. Yet, iPSCs form large cell aggregates in suspension bioreactors, resulting in insufficient nutrient supply and extra metabolic waste build-up for the cells located at the core. Since subtle changes in micro-environment can lead to a heterogeneous cell population, a novel Biological System-of-Systems (Bio-SoS) framework is proposed to model cell-to-cell interactions, spatial and metabolic heterogeneity, and cell response to micro-environmental variation...
January 8, 2024: Communications Biology
https://read.qxmd.com/read/38191311/impact-of-the-covid-19-pandemic-on-prehospital-and-in-hospital-treatment-and-outcomes-of-patients-after-out-of-hospital-cardiac-arrest-a-japanese-multicenter-cohort-study
#36
JOURNAL ARTICLE
Chie Tanaka, Takashi Tagami, Junya Kaneko, Nobuya Kitamura, Hideo Yasunaga, Shotaro Aso, Munekazu Takeda, Masamune Kuno
BACKGROUND: In the chain of survival for Out-of-hospital cardiac arrest (OHCA), each component of care contributes to improve the prognosis of the patient with OHCA. The SARS-CoV-2 (COVID-19) pandemic potentially affected each part of care in the chain of survival. The aim of this study was to compare prehospital care, in-hospital treatment, and outcomes among OHCA patients before and after the COVID-19 pandemic. METHODS: We analyzed data from a multicenter prospective study in Kanto area, Japan, named SOS-KANTO 2017...
January 8, 2024: BMC Emergency Medicine
https://read.qxmd.com/read/38176302/evaluating-the-diagnostic-performance-of-liaison%C3%A2-chemiluminescence-assay-as-screening-tool-for-detection-of-acute-epstein-barr-infection-a-comparative-study
#37
JOURNAL ARTICLE
Jordi Tortosa-Carreres, Carmen Lloret-Sos, Jose Miguel Sahuquillo-Arce, Pedro Suárez-Urquiza, Josep Prat-Fornells, Jose Miguel Molina-Moreno, Amparo Alba-Redondo, Maria Luisa Martínez-Triguero, Cristina Aguado-Codina, Begoña Laiz-Marro, Jose Luis López-Hontangas
The present investigation assessed the Liaison® diagnostic performance in detecting Epstein-Barr (EBV) IgM-VCA in a large patient cohort, considering age and symptomatology. VIDAS® were employed as a benchmark for acute EBV infection. The study also probed other coexisting conditions and potential cross-reactivity for error sources. A total of 1311 samples were analyzed, with notable associations found only among paediatric (kappa=0.75) and young adult (kappa=0.58) populations with compatible symptoms...
December 24, 2023: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38164997/d-dimer-and-sinusoidal-obstructive-syndrome-novel-poor-prognostic-features-of-thrombotic-microangiopathy-in-children-after-hematopoietic-cellular-therapy-in-a-single-institution-prospective-cohort-study
#38
JOURNAL ARTICLE
Michelle L Schoettler, Kaley French, Anora Harris, Elyse Bryson, Laura Deeb, Zuri Hudson, Jeremy Obordo, Shanmuganathan Chandrakasan, Suhag Parikh, Benjamin Watkins, Elizabeth Stenger, Muna Qayed, Satheesh Chonat, Adrianna Westbrook, Jeffrey Switchenko, Kirsten M Williams
Transplant-associated thrombotic microangiopathy (TA-TMA) is a common, severe complication of allogeneic hematopoietic cellular therapy (HCT). Even when treated in many studies, morbidity and mortality rates are high. This prospective single-institution cohort study serially enrolled all allogeneic HCT recipients from August 2019-August 2022. Patients were universally screened for TA-TMA and intermediate and high-risk patients were immediately treated with eculizumab. Sub-distribution cox-proportional hazards models were used to identify sub-distribution hazard ratios (sHR)  for multi-organ dysfunction (MOD) and non-relapse-related mortality (NRM)...
January 2, 2024: American Journal of Hematology
https://read.qxmd.com/read/38163543/ofloxacin-weakened-treatment-performance-of-rural-domestic-sewage-in-an-aerobic-biofilm-system-by-affecting-biofilm-resistance-bacterial-community-and-functional-genes
#39
JOURNAL ARTICLE
Bingbing Feng, Juan Chen, Chao Wang, Guoxiang You, Junkai Lin, Han Gao, Shanrui Han, Junchao Ma
Ofloxacin (OFL) is a typical fluoroquinolone antibiotic widely detected in rural domestic sewage, however, its effects on the performance of aerobic biofilm systems during sewage treatment process remain poorly understood. We carried out an aerobic biofilm experiment to explore how the OFL with different concentrations affects the pollutant removal efficiency of rural domestic sewage. Results demonstrated that the OFL negatively affected pollutant removal in aerobic biofilm systems. High OFL levels resulted in a decrease in removal efficiency: 9...
December 30, 2023: Environmental Research
https://read.qxmd.com/read/38160879/biofilms-a-developmental-niche-for-vancomycin-intermediate-staphylococcus-aureus
#40
JOURNAL ARTICLE
Jenelle E Chapman, Shilpa E George, Christiane Wolz, Michael E Olson
Staphylococcus aureus are gram-positive bacteria responsible for a wide array of diseases, ranging from skin and soft tissue infections to more chronic illnesses such as toxic shock syndrome, osteomyelitis, and endocarditis. Vancomycin is currently one of the most effective antibiotics available in treating patients infected with methicillin-resistant S. aureus (MRSA), however the emergence of vancomycin-resistant S. aureus (VRSA), and more commonly vancomycin-intermediate S. aureus (VISA), threaten the future efficacy of vancomycin...
January 2024: Infection, Genetics and Evolution
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