keyword
https://read.qxmd.com/read/38546227/-bacillus-subtilis-remains-translationally-active-after-crispri-mediated-replication-initiation-arrest
#1
JOURNAL ARTICLE
Vanessa Muñoz-Gutierrez, Fabián A Cornejo, Katja Schmidt, Christian K Frese, Manuel Halte, Marc Erhardt, Alexander K W Elsholz, Kürşad Turgay, Emmanuelle Charpentier
Initiation of bacterial DNA replication takes place at the origin of replication ( oriC ), a region characterized by the presence of multiple DnaA boxes that serve as the binding sites for the master initiator protein DnaA. This process is tightly controlled by modulation of the availability or activity of DnaA and oriC during development or stress conditions. Here, we aimed to uncover the physiological and molecular consequences of stopping replication in the model bacterium Bacillus subtilis . We successfully arrested replication in B...
March 28, 2024: MSystems
https://read.qxmd.com/read/38540741/sos-inducing-drugs-trigger-nucleic-acid-release-and-biofilm-formation-in-gram-negative-bacteria
#2
JOURNAL ARTICLE
Peter Demjanenko, Sam Zheng, John K Crane
Our laboratory recently reported that induction of the SOS response, triggered by SOS-inducing drugs, was accompanied by a large release of DNA from enteric bacteria. The SOS response release had not previously been reported to include release of extracellular DNA from bacterial cells. We followed up on those observations in this current study and found that not just double-stranded DNA was being released, but also single-stranded DNA, RNA, and protein. SOS-inducing drugs also triggered formation of biofilm at the air-fluid interface on glass, and the biofilms contained DNA...
March 8, 2024: Biomolecules
https://read.qxmd.com/read/38540680/the-configuration-of-grb2-in-protein-interaction-and-signal-transduction
#3
REVIEW
Dingyi Wang, Guoxia Liu, Yuxin Meng, Hongjie Chen, Zu Ye, Ji Jing
Growth-factor-receptor-binding protein 2 (GRB2) is a non-enzymatic adaptor protein that plays a pivotal role in precisely regulated signaling cascades from cell surface receptors to cellular responses, including signaling transduction and gene expression. GRB2 binds to numerous target molecules, thereby modulating a complex cell signaling network with diverse functions. The structural characteristics of GRB2 are essential for its functionality, as its multiple domains and interaction mechanisms underpin its role in cellular biology...
February 22, 2024: Biomolecules
https://read.qxmd.com/read/38540185/xylosyltransferase-deficiency-in-human-dermal-fibroblasts-induces-compensatory-myofibroblast-differentiation-and-long-term-ecm-reduction
#4
JOURNAL ARTICLE
Anika Kleine, Matthias Kühle, Thanh-Diep Ly, Vanessa Schmidt, Isabel Faust-Hinse, Cornelius Knabbe, Bastian Fischer
Desbuquois dysplasia type 2 (DBQD2) and spondylo-ocular syndrome (SOS) are autosomal recessive disorders affecting the extracellular matrix (ECM) and categorized as glycosaminoglycan (GAG) linkeropathies. Linkeropathies result from mutations within glycosyltransferases involved in the synthesis of the tetrasaccharide linker, a linker between the core protein of proteoglycan (PG) and GAG. DBQD2 and SOS are caused by the isolated mutations of the xylosyltransferase (XT) isoforms. In this work, we successfully generated XYLT1 - as well as XYLT2 -deficient GAG linkeropathy model systems in human dermal fibroblasts using a ribonucleoprotein-based CRISPR/Cas9-system...
March 4, 2024: Biomedicines
https://read.qxmd.com/read/38536908/tisb-protein-is-the-single-molecular-determinant-underlying-multiple-downstream-effects-of-ofloxacin-in-escherichia-coli
#5
JOURNAL ARTICLE
Julien Cayron, Thierry Oms, Tatjana Schlechtweg, Safia Zedek, Laurence Van Melderen
Bactericidal antibiotics can cause metabolic perturbations that contribute to antibiotic-induced lethality. The molecular mechanism underlying these downstream effects remains unknown. Here, we show that ofloxacin, a fluoroquinolone that poisons DNA gyrase, induces a cascade of metabolic changes that are dependent on an active SOS response. We identified the SOS-regulated TisB protein as the unique molecular determinant responsible for cytoplasmic condensation, proton motive force dissipation, loss of pH homeostasis, and H2 O2 accumulation in Escherichia coli cells treated with high doses of ofloxacin...
March 29, 2024: Science Advances
https://read.qxmd.com/read/38534711/sub-mic-antibiotics-modulate-productions-of-outer-membrane-vesicles-in-tigecycline-resistant-escherichia-coli
#6
JOURNAL ARTICLE
Qianru Li, Jun Li, Tao He, Xing Ji, Ruicheng Wei, Meiling Yu, Ran Wang
Antimicrobial resistance (AMR) has been recognized as one of the most important crises affecting global human health in the 21st century. Tigecycline is one of the last resort antibiotics for treating severe infections caused by multi-drug resistant Enterobacteriaceae . However, the mobile resistance gene tet (X4), which could mediate high-level tigecycline resistance, was discovered in 2019. The outer membrane vesicle (OMV) has been recognized as a new route for horizontal gene transfer; antimicrobial resistant bacteria also have the ability to secret OMVs, while little is known about the impact of antibiotics on the secretion and characteristics of OMVs from tigecycline resistant bacteria till now...
March 19, 2024: Antibiotics
https://read.qxmd.com/read/38534113/exploring-the-links-between-sos-response-mutagenesis-and-resistance-during-the-recovery-period
#7
JOURNAL ARTICLE
Sreyashi Ghosh, Mehmet A Orman
Although the mechanistic connections between SOS-induced mutagenesis and antibiotic resistance are well established, our current understanding of the impact of SOS response levels, recovery durations, and transcription/translation activities on mutagenesis remains relatively limited. In this study, when bacterial cells were exposed to mutagens like ultraviolet light for defined time intervals, a compelling connection between the rate of mutagenesis and the RecA-mediated SOS response levels became evident. Our observations also indicate that mutagenesis primarily occurs during the subsequent recovery phase following the removal of the mutagenic agent...
March 27, 2024: Antimicrobial Agents and Chemotherapy
https://read.qxmd.com/read/38531448/responses-of-phenology-to-preseason-drought-and-soil-temperature-for-different-land-cover-types-on-the-mongolian-plateau
#8
JOURNAL ARTICLE
Bing Li, Rong Wang, Jing M Chen
Drought and heat caused major disturbance in nature by interfering with plant phenology, and can also alter the vulnerability and resilience of terrestrial ecosystems. Existing research on the Mongolian Plateau has primarily focused on studying the response of the start (SOS) and end (EOS) of the growing season to drought and heat variations. However, there is still a lack of comprehensive understanding regarding the coupled effects of drought and heat on phenology across different land cover types. In this study, we retrieved SOS and EOS based on 34-year (1982-2015) normalized difference vegetation index (NDVI) dataset from Global Inventory Modeling and Mapping Studies (GIMMS)...
March 24, 2024: Science of the Total Environment
https://read.qxmd.com/read/38524386/orthopaedic-hand-patient-support-systems-have-valuable-insight-to-patient-function-and-pain
#9
JOURNAL ARTICLE
E A Washnock-Schmid, Nicholas Livingston, Katie Latack, Nancy Wrobel, Charles S Day
Patient-reported outcome measures (PROs) are increasingly used in clinical assessment. Research on how patient support systems contribute to physician understanding of patient condition is limited. Thus, insights from significant others may provide value, especially when concerns exist regarding patient response validity. Patients recruited from the pre-operative environment undergoing orthopaedic hand procedures responded to PROMIS-Pain Interference (PI), PROMIS-Upper Extremity (UE), PROMIS-Depression (D), and QuickDASH...
2024: Journal of Patient Experience
https://read.qxmd.com/read/38515535/isolation-of-feline-islets-of-langerhans-by-selective-osmotic-shock-produces-glucose-responsive-islets
#10
JOURNAL ARTICLE
Lauren T Porter, Christopher A Adin, Chiquitha D Crews, Jocelyn Mott, Chen Gilor
INTRODUCTION: Pancreatic islet isolation is essential for studying islet physiology, pathology, and transplantation, and feline islets could be an important model for human type II diabetes mellitus (T2D). Traditional isolation methods utilizing collagenases inflict damage and, in cats, may contribute to the difficulty in generating functional islets, as demonstrated by glucose-stimulated insulin secretion (GSIS). GLUT2 expression in β cells may allow for adaptation to hyperosmolar glucose solutions while exocrine tissue is selectively disrupted...
2024: Frontiers in Veterinary Science
https://read.qxmd.com/read/38513945/%C3%AE-cyclocitral-induced-rapid-cell-death-of-microcystis-aeruginosa
#11
JOURNAL ARTICLE
Xuejian Wang, Huansheng Cao, Yinjie Zhu, Tingru Zhou, Fei Teng, Yi Tao
β-cyclocitral (BCC) is an odorous compound that can be produced by bloom-forming cyanobacteria, for example, Microcystis aeruginosa. BCC has been proposed to explain the rapid decline of cyanobacterial blooms in natural water bodies due to its lytic effects on cyanobacteria cells. However, few insights have been gained regarding the mechanisms of its lethality on cyanobacteria. In this study, M. aeruginosa was exposed to 0-300 mg/L BCC, and the physiological responses were comprehensively studied at the cellular, molecular, and transcriptomic levels...
March 19, 2024: Environmental Pollution
https://read.qxmd.com/read/38513314/second-order-scattering-sensor-based-on-the-zn-0-97-la-0-03-o-compound-for-selective-and-stable-detection-of-glycated-albumin
#12
JOURNAL ARTICLE
José Jonathan Soares Dos Santos, Ramon Raudel Peña Garcia, Adriano Santana Soares, Elias Gabriel de Amorim Silva, Jorge Luiz Neves, Thaís Meira Menezes
Here, we presented a second-order scattering sensor based on the Zn0.97 La0.03 O compound (LaZnO) for selective and stable detection of glycated albumin (GA, glycemic long-term biomarker). The LaZnO sample was obtained through the co-precipitation method and then characterized using microscopic and spectroscopic techniques. Furthermore, the selectivity, molecular interference, temporal stability, and pH effects of the LaZnO SOS signal in the absence and presence of GA were investigated. The results indicate the stability of the SOS signal over more than 60 days...
March 19, 2024: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://read.qxmd.com/read/38506894/investigating-the-roles-and-responsibilities-of-institutional-signing-officials-after-data-sharing-policy-reform-for-federally-funded-research-in-the-united-states-national-survey
#13
JOURNAL ARTICLE
Jinyoung Baek, Jonathan Lawson, Vasiliki Rahimzadeh
BACKGROUND: New federal policies along with rapid growth in data generation, storage, and analysis tools are together driving scientific data sharing in the United States. At the same, triangulating human research data from diverse sources can also create situations where data are used for future research in ways that individuals and communities may consider objectionable. Institutional gatekeepers, namely, signing officials (SOs), are therefore at the helm of compliant management and sharing of human data for research...
March 20, 2024: JMIR Formative Research
https://read.qxmd.com/read/38498495/spatial-variation-in-responses-of-plant-spring-phenology-to-climate-warming-in-grasslands-of-inner-mongolia-drivers-and-application
#14
JOURNAL ARTICLE
Guang Lu, Mengchao Fang, Shuping Zhang
Plant spring phenology in grasslands distributed in the Northern Hemisphere is highly responsive to climate warming. The growth of plants is intricately influenced by not only air temperature but also precipitation and soil factors, both of which exhibit spatial variation. Given the critical impact of the plant growth season on the livelihood of husbandry communities in grasslands, it becomes imperative to comprehend regional-scale spatial variation in the response of plant spring phenology to climate warming and the effects of precipitation and soil factors on such variation...
February 14, 2024: Plants (Basel, Switzerland)
https://read.qxmd.com/read/38496562/proteomic-predictors-of-individualized-nutrient-specific-insulin-secretion-in-health-and-disease
#15
Jelena Kolic, WenQing Grace Sun, Haoning Howard Cen, Jessica Ewald, Jason C Rogalski, Shugo Sasaki, Han Sun, Varsha Rajesh, Yi Han Xia, Renata Moravcova, Søs Skovsø, Aliya F Spigelman, Jocelyn E Manning Fox, James Lyon, Leanne Beet, Jianguo Xia, Francis C Lynn, Anna L Gloyn, Leonard J Foster, Patrick E MacDonald, James D Johnson
UNLABELLED: Population level variation and molecular mechanisms behind insulin secretion in response to carbohydrate, protein, and fat remain uncharacterized despite ramifications for personalized nutrition. Here, we define prototypical insulin secretion dynamics in response to the three macronutrients in islets from 140 cadaveric donors, including those diagnosed with type 2 diabetes. While islets from the majority of donors exhibited the expected relative response magnitudes, with glucose being highest, amino acid moderate, and fatty acid small, 9% of islets stimulated with amino acid and 8% of islets stimulated with fatty acids had larger responses compared with high glucose...
March 5, 2024: medRxiv
https://read.qxmd.com/read/38485102/physicochemical-properties-of-26-carbon-nanotubes-as-predictors-for-pulmonary-inflammation-and-acute-phase-response-in-mice-following-intratracheal-lung-exposure
#16
JOURNAL ARTICLE
Pernille Høgh Danielsen, Sarah Søs Poulsen, Kristina Bram Knudsen, Per Axel Clausen, Keld Alstrup Jensen, Håkan Wallin, Ulla Vogel
Carbon nanotubes (CNTs) vary in physicochemical properties which makes risk assessment challenging. Mice were pulmonary exposed to 26 well-characterized CNTs using the same experimental design and followed for one day, 28 days or 3 months. This resulted in a unique dataset, which was used to identify physicochemical predictors of pulmonary inflammation and systemic acute phase response. MWCNT diameter and SWCNT specific surface area were predictive of lower and higher neutrophil influx, respectively. Manganese and iron were shown to be predictive of higher neutrophil influx at day 1 post-exposure, whereas nickel content interestingly was predictive of lower neutrophil influx at all three time points and of lowered acute phase response at day 1 and 3 months post-exposure...
March 12, 2024: Environmental Toxicology and Pharmacology
https://read.qxmd.com/read/38470036/the-intrinsically-disordered-linker-in-the-single-stranded-dna-binding-protein-influences-dna-replication-restart-and-recombination-pathways-in-escherichia-coli-k-12
#17
JOURNAL ARTICLE
Steven J Sandler, Nina J Bonde, Elizabeth A Wood, Michael M Cox, James L Keck
Tetrameric single-stranded (ss) DNA-binding proteins (SSBs) stabilize ssDNA intermediates formed during genome maintenance reactions in Bacteria . SSBs also recruit proteins important for these processes through direct SSB-protein interactions, including proteins involved in DNA replication restart and recombination processes. SSBs are composed of an N-terminal oligomerization and ssDNA-binding domain, a C-terminal acidic tip that mediates SSB-protein interactions, and an internal intrinsically disordered linker (IDL)...
March 12, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38466718/widespread-prevalence-of-a-methylation-dependent-switch-to-activate-an-essential-dna-damage-response-in-bacteria
#18
JOURNAL ARTICLE
Aditya Kamat, Ngat T Tran, Mohak Sharda, Neha Sontakke, Tung B K Le, Anjana Badrinarayanan
DNA methylation plays central roles in diverse cellular processes, ranging from error-correction during replication to regulation of bacterial defense mechanisms. Nevertheless, certain aberrant methylation modifications can have lethal consequences. The mechanisms by which bacteria detect and respond to such damage remain incompletely understood. Here, we discover a highly conserved but previously uncharacterized transcription factor (Cada2), which orchestrates a methylation-dependent adaptive response in Caulobacter...
March 11, 2024: PLoS Biology
https://read.qxmd.com/read/38449342/sporadic-regional-re-emergent-cholera-a-19th-century-problem-in-the-21st-century
#19
JOURNAL ARTICLE
Ibrahim S I Al-Adham, Nisrein Jaber, Ahmed S A Ali Agha, Mayyas Al-Remawi, Faisal Al-Akayleh, Najah Al-Muhtaseb, Phillip J Collier
Cholera, caused by Vibrio cholerae, is a severe diarrheal disease that necessitates prompt diagnosis and effective treatment. This review comprehensively examines various diagnostic methods, from traditional microscopy and culture to advanced nucleic acid testing like Polymerase Spiral Reaction (PSR) and Rapid Diagnostic Tests (RDTs), highlighting their advantages and limitations. Additionally, we explore evolving treatment strategies, with a focus on the challenges posed by antibiotic resistance due to the activation of the SOS response pathway in V...
March 6, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38438143/adaptive-evolution-of-plasmid-and-chromosome-contributes-to-the-fitness-of-a-blandm-bearing-cointegrate-plasmid-in-escherichia-coli
#20
JOURNAL ARTICLE
Ziyi Liu, Yanyun Gao, Mianzhi Wang, Yuan Liu, Fulin Wang, Jing Shi, Zhiqiang Wang, Ruichao Li
Large cointegrate plasmids recruit genetic features of their parental plasmids and serve as important vectors in the spread of antibiotic resistance. They are now frequently found in clinical settings, raising the issue of how to limit their further transmission. Here, we conducted evolutionary research of a large blaNDM positive cointegrate within Escherichia coli C600, and discovered that adaptive evolution of chromosome and plasmid jointly improved bacterial fitness, which was manifested as enhanced survival ability for in vivo and in vitro pairwise competition, biofilm formation, and gut colonization ability...
March 4, 2024: ISME Journal
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