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Mixed species biofilm

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https://www.readbyqxmd.com/read/27868360/global-transcriptome-responses-including-small-rnas-during-mixed-species-interactions-with-methicillin-resistant-staphylococcus-aureus-and-pseudomonas-aeruginosa
#1
Christine L Miller, Tricia A Van Laar, Tsute Chen, S L Rajasekhar Karna, Ping Chen, Tao You, Kai P Leung
Pseudomonas aeruginosa and Staphylococcus aureus mixed-species biofilm infections are more resilient to biocide attacks compared to their single-species counterparts. Therefore, this study used an in vitro model recapitulating bacterial burdens seen in in vivo infections to investigate the interactions of P. aeruginosa and S. aureus in biofilms. RNA sequencing (RNA-seq) was utilized to identify the entire genomic response, both open reading frames (ORFs) and small RNAs (sRNAs), of each species. Using competitive indexes, transposon mutants validated uncharacterized PA1595 of P...
November 21, 2016: MicrobiologyOpen
https://www.readbyqxmd.com/read/27849175/two-isoforms-of-clp-peptidase-in-pseudomonas-aeruginosa-control-distinct-aspects-of-cellular-physiology
#2
Branwen M Hall, Elena B M Breidenstein, César de la Fuente-Núñez, Fany Reffuveille, Gina D Mawla, Robert E W Hancock, Tania A Baker
Caseinolytic peptidases (ClpPs) regulate diverse aspects of cellular physiology in bacteria. Some species have multiple ClpPs including opportunistic pathogen Pseudomonas aeruginosa in which there is an archetypical isoform, ClpP1, and a second isoform, ClpP2, about which little is known. Here we use phenotypic assays to investigate biological roles of ClpP1 and ClpP2 and biochemical assays to characterize purified ClpP1, ClpP2, ClpX and ClpA. Interestingly ClpP1 and ClpP2 have distinct intracellular roles for motility, pigment production, iron scavenging and biofilm formation...
November 14, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27836851/the-behavior-of-staphylococcus-aureus-dual-species-biofilms-treated-with-bacteriophage-phiipla-rodi-depends-on-the-accompanying-microorganism
#3
Silvia González, Lucía Fernández, Ana Belén Campelo, Diana Gutiérrez, Beatriz Martínez, Ana Rodríguez, Pilar García
: The use of bacteriophages as antimicrobials against pathogenic bacteria offers a promising alternative to traditional antibiotics and disinfectants. Significantly, phages may help to remove biofilms, which are notoriously resistant to commonly used eradication methods. However, the successful development of novel antibiofilm strategies must take into account that real-life biofilms usually consist of mixed-species populations. Within this context, this study aimed to explore the effectiveness of bacteriophage-based sanitation procedures for removing polymicrobial biofilms from food industry surfaces...
November 11, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27803696/new-weapons-to-fight-old-enemies-novel-strategies-for-the-bio-control-of-bacterial-biofilms-in-the-food-industry
#4
REVIEW
Laura M Coughlan, Paul D Cotter, Colin Hill, Avelino Alvarez-Ordóñez
Biofilms are microbial communities characterized by their adhesion to solid surfaces and the production of a matrix of exopolymeric substances, consisting of polysaccharides, proteins, DNA and lipids, which surround the microorganisms lending structural integrity and a unique biochemical profile to the biofilm. Biofilm formation enhances the ability of the producer/s to persist in a given environment. Pathogenic and spoilage bacterial species capable of forming biofilms are a significant problem for the healthcare and food industries, as their biofilm-forming ability protects them from common cleaning processes and allows them to remain in the environment post-sanitation...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27770039/a-critical-role-for-extracellular-dna-in-dental-plaque-formation
#5
N Rostami, R C Shields, S A Yassin, A R Hawkins, L Bowen, T L Luo, A H Rickard, R Holliday, P M Preshaw, N S Jakubovics
Extracellular DNA (eDNA) has been identified in the matrix of many different monospecies biofilms in vitro, including some of those produced by oral bacteria. In many cases, eDNA stabilizes the structure of monospecies biofilms. Here, the authors aimed to determine whether eDNA is an important component of natural, mixed-species oral biofilms, such as plaque on natural teeth or dental implants. To visualize eDNA in oral biofilms, approaches for fluorescently stained eDNA with either anti-DNA antibodies or an ultrasensitive cell-impermeant dye, YOYO-1, were first developed using Enterococcus faecalis, an organism that has previously been shown to produce extensive eDNA structures within biofilms...
October 21, 2016: Journal of Dental Research
https://www.readbyqxmd.com/read/27761322/antibacterial-and-anti-adherence-effects-of-a-plant-extract-mixture-pem-and-its-individual-constituent-extracts-psidium-sp-mangifera-sp-and-mentha-sp-on-single-and-dual-species-biofilms
#6
Zaleha Shafiei, Zubaidah Haji Abdul Rahim, Koshy Philip, Nalina Thurairajah
BACKGROUND: Plant extracts mixture (PEM) and its individual constituent plant extracts(Psidium sp., Mangifera sp., Mentha sp.) are known to have an anti-adhering effect towards oral bacteria in the single-species biofilm. To date, the adhering ability of the early and late plaque colonisers (Streptococcus sanguinis and Streptococcus mutans) to PEM-treated experimental pellicle have not been investigated in dual-species biofilms. METHODS: Fresh leaves of these plants were used in the preparation of the respective aqueous extract decoctions...
2016: PeerJ
https://www.readbyqxmd.com/read/27759365/iron-complex-facilitated-copper-redox-cycling-for-nitric-oxide-generation-as-non-toxic-nitrifying-biofilm-inhibitor
#7
Vita Wonoputri, Cindy Gunawan, Sanly Liu, Nicolas Barraud, Lachlan Hartley Yee, May Lim, Rose Amal
In this study, we developed a poly(vinyl chloride) (PVC)-solvent casted mixed metal copper and iron complexes capable of catalytic generation of the antibiofilm nitric oxide (NO) from endogenous nitrite. In the absence of additional reducing agent, we demonstrated that the presence of iron complex facilitates a redox cycling, converting the copper(II) complex to active copper(I) species, which catalyzes the generation of NO from nitrite. Assessed by protein assay and surface coverage analyses, the presence of the mixed metal complexes in systems containing water industry-relevant nitrite-producing nitrifying biofilms was shown to result in a 'non-toxic mode' of biofilm suppression, while confining the bacterial growth to the free-floating planktonic phase...
October 19, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27756620/virulence-arsenal-of-the-most-pathogenic-species-among-the-gram-positive-anaerobic-cocci-finegoldia-magna
#8
REVIEW
Lyudmila Boyanova, Rumyana Markovska, Ivan Mitov
This review focuses on the virulence arsenal of the most pathogenic species among Gram positive anaerobic cocci, Finegoldia magna according to recently published data from 2012 to 2016. Virulence factors like sortase dependent pili and F. magna adhesion factor (FAF) facilitate the start of the infection. Albumin binding protein (PAB) enhances F. magna survival. FAF, subtilisin-like extracellular serine protease (SufA) and superantigen protein L protect the bacteria from factors of innate defense system. SufA, capsule and tissue-destroying enzymes provide a deep penetration or spread of the infections and the protein L is associated with infection severity...
October 15, 2016: Anaerobe
https://www.readbyqxmd.com/read/27736645/enterococcal-metabolite-cues-facilitate-interspecies-niche-modulation-and-polymicrobial-infection
#9
Damien Keogh, Wei Hong Tay, Yao Yong Ho, Jennifer L Dale, Siyi Chen, Shivshankar Umashankar, Rohan B H Williams, Swaine L Chen, Gary M Dunny, Kimberly A Kline
Enterococcus faecalis is frequently associated with polymicrobial infections of the urinary tract, indwelling catheters, and surgical wound sites. E. faecalis co-exists with Escherichia coli and other pathogens in wound infections, but mechanisms that govern polymicrobial colonization and pathogenesis are poorly defined. During infection, bacteria must overcome multiple host defenses, including nutrient iron limitation, to persist and cause disease. In this study, we investigated the contribution of E. faecalis to mixed-species infection when iron availability is restricted...
October 12, 2016: Cell Host & Microbe
https://www.readbyqxmd.com/read/27729510/commensal-protection-of-staphylococcus-aureus-against-antimicrobials-by-candida-albicans-biofilm-matrix
#10
Eric F Kong, Christina Tsui, Sona Kucharíková, David Andes, Patrick Van Dijck, Mary Ann Jabra-Rizk
: Biofilm-associated polymicrobial infections, particularly those involving fungi and bacteria, are responsible for significant morbidity and mortality and tend to be challenging to treat. Candida albicans and Staphylococcus aureus specifically are considered leading opportunistic fungal and bacterial pathogens, respectively, mainly due to their ability to form biofilms on catheters and indwelling medical devices. However, the impact of mixed-species biofilm growth on therapy remains largely understudied...
October 11, 2016: MBio
https://www.readbyqxmd.com/read/27706381/culture-media-profoundly-affect-candida-albicans-and-candida-tropicalis-growth-adhesion-and-biofilm-development
#11
Manjula M Weerasekera, Gayan K Wijesinghe, Thilini A Jayarathna, Chinthika P Gunasekara, Neluka Fernando, Nilwala Kottegoda, Lakshman P Samaranayake
As there are sparse data on the impact of growth media on the phenomenon of biofilm development for Candida we evaluated the efficacy of three culture media on growth, adhesion and biofilm formation of two pathogenic yeasts, Candida albicans and Candida tropicalis. The planktonic phase yeast growth, either as monocultures or mixed cultures, in sabouraud dextrose broth (SDB), yeast nitrogen base (YNB), and RPMI 1640 was compared, and adhesion as well as biofilm formation were monitored using MTT and crystal violet (CV) assays and scanning electron microscopy...
November 2016: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/27702795/prevention-of-polymicrobial-biofilms-composed-of-pseudomonas-aeruginosa-and-pathogenic-fungi-by-essential-oils-from-selected-citrus-species
#12
Marina Pekmezovic, Ivana Aleksic, Aleksandra Barac, Valentina Arsic-Arsenijevic, Branka Vasiljevic, Jasmina Nikodinovic-Runic, Lidija Senerovic
Mixed microbial infections caused by Pseudomonas aeruginosa and pathogenic fungi are commonly found in patients with chronic infections and constitute a significant health care burden. The aim of this study was to address the potential polymicrobial antibiofilm activity of pompia and grapefruit essential oils (EOs). The mechanism of antimicrobial activity of EOs was analysed. EOs of pompia and grapefruit inhibited fungal growth with MIC concentrations between 50 and 250 mg L(-1), whereas no effect on P. aeruginosa growth was observed...
November 2016: Pathogens and Disease
https://www.readbyqxmd.com/read/27699574/effect-of-bioactive-dental-adhesive-on-periodontal-and-endodontic-pathogens
#13
Lin Wang, Xianju Xie, Michael D Weir, Ashraf F Fouad, Liang Zhao, Hockin H K Xu
The objectives of this study were to: (1) develop a new bioactive dental bonding agent with nanoparticles of amorphous calcium phosphate and dimethylaminohexadecyl methacrylate for tooth root caries restorations and endodontic applications, and (2) investigate biofilm inhibition by the bioactive bonding agent against eight species of periodontal and endodontic pathogens for the first time. Bonding agent was formulated with 5 % of dimethylaminohexadecyl methacrylate. Nanoparticles of amorphous calcium phosphate at 30 wt% was mixed into adhesive...
November 2016: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/27671471/novel-bioactive-nanocomposite-for-class-v-restorations-to-inhibit-periodontitis-related-pathogens
#14
Lin Wang, Mary A S Melo, Michael D Weir, Xianju Xie, Mark A Reynolds, Hockin H K Xu
OBJECTIVES: The occurrence of tooth root caries is increasing as the world population ages and tooth retention in seniors increases. Class V restorations with subgingival margins are difficult to clean and often lead to periodontitis. The objectives of this study were to develop a Class V composite containing dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP), and investigate mechanical properties and the inhibition of six species of periodontitis-related biofilms for the first time...
December 2016: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/27651359/bacteriome-and-mycobiome-interactions-underscore-microbial-dysbiosis-in-familial-crohn-s-disease
#15
G Hoarau, P K Mukherjee, C Gower-Rousseau, C Hager, J Chandra, M A Retuerto, C Neut, S Vermeire, J Clemente, J F Colombel, H Fujioka, D Poulain, B Sendid, M A Ghannoum
UNLABELLED: Crohn's disease (CD) results from a complex interplay between host genetic factors and endogenous microbial communities. In the current study, we used Ion Torrent sequencing to characterize the gut bacterial microbiota (bacteriome) and fungal community (mycobiome) in patients with CD and their nondiseased first-degree relatives (NCDR) in 9 familial clusters living in northern France-Belgium and in healthy individuals from 4 families living in the same area (non-CD unrelated [NCDU])...
September 20, 2016: MBio
https://www.readbyqxmd.com/read/27648759/chemical-physical-and-morphological-properties-of-bacterial-biofilms-affect-survival-of-encased-campylobacter-jejuni-f38011-under-aerobic-stress
#16
Jinsong Feng, Guillaume Lamour, Rui Xue, Mehr Negar Mirvakliki, Savvas G Hatzikiriakos, Jie Xu, Hongbin Li, Shuo Wang, Xiaonan Lu
Campylobacter jejuni is a microaerophilic pathogen and leading cause of human gastroenteritis. The presence of C. jejuni encased in biofilms found in meat and poultry processing facilities may be the major strategy for its survival and dissemination in aerobic environment. In this study, Staphylococcus aureus, Salmonella enterica, or Pseudomonas aeruginosa was mixed with C. jejuni F38011 as a culture to form dual-species biofilms. After 4days' exposure to aerobic stress, no viable C. jejuni cells could be detected from mono-species C...
December 5, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27623781/the-influence-of-prior-modes-of-growth-temperature-medium-and-substrate-surface-on-biofilm-formation-by-antibiotic-resistant-campylobacter-jejuni
#17
Amy Huei Teen Teh, Sui Mae Lee, Gary A Dykes
Campylobacter jejuni is one of the most common causes of bacterial gastrointestinal food-borne infection worldwide. It has been suggested that biofilm formation may play a role in survival of these bacteria in the environment. In this study, the influence of prior modes of growth (planktonic or sessile), temperatures (37 and 42 °C), and nutrient conditions (nutrient broth and Mueller-Hinton broth) on biofilm formation by eight C. jejuni strains with different antibiotic resistance profiles was examined. The ability of these strains to form biofilm on different abiotic surfaces (stainless steel, glass, and polystyrene) as well as factors potentially associated with biofilm formation (bacterial surface hydrophobicity, auto-aggregation, and initial attachment) was also determined...
December 2016: Current Microbiology
https://www.readbyqxmd.com/read/27623227/rhamnolipids-mediate-an-interspecies-biofilm-dispersal-signaling-pathway
#18
Arunima Bhattacharjee, Tyler D Nusca, Allon I Hochbaum
Bacterial biofilms are problematic in natural and anthropogenic environments, and they confer protective properties on their constituent cells, making them difficult to treat with conventional antibiotics. Anti-biofilm strategies, therefore, represent a promising direction of research for treating biofilm infections. Natural autodispersal and interspecies dispersal signaling pathways provide insight into cell-cell communication mechanisms, species dynamics in mixed communities, and potential targets for infection therapies...
September 13, 2016: ACS Chemical Biology
https://www.readbyqxmd.com/read/27601572/redefining-the-chronic-wound-microbiome-fungal-communities-are-prevalent-dynamic-and-associated-with-delayed-healing
#19
Lindsay Kalan, Michael Loesche, Brendan P Hodkinson, Kristopher Heilmann, Gordon Ruthel, Sue E Gardner, Elizabeth A Grice
UNLABELLED: Chronic nonhealing wounds have been heralded as a silent epidemic, causing significant morbidity and mortality especially in elderly, diabetic, and obese populations. Polymicrobial biofilms in the wound bed are hypothesized to disrupt the highly coordinated and sequential events of cutaneous healing. Both culture-dependent and -independent studies of the chronic-wound microbiome have almost exclusively focused on bacteria, omitting what we hypothesize are important fungal contributions to impaired healing and the development of complications...
September 6, 2016: MBio
https://www.readbyqxmd.com/read/27552083/enemies-and-brothers-in-arms-candida-albicans-and-gram-positive-bacteria
#20
REVIEW
Toni M Förster, Selene Mogavero, Antonia Dräger, Katja Graf, Melanie Polke, Ilse D Jacobsen, Bernhard Hube
Candida albicans is an important human opportunistic fungal pathogen which is frequently found as part of the normal human microbiota. It is well accepted that the fungus interacts with other components of the resident microbiota and that this impacts the commensal or pathogenic outcome of C. albicans colonization. Different types of interactions, including synergism or antagonism, contribute to a complex balance between the multitude of different species. Mixed biofilms of C. albicans and streptococci are a well-studied example of a mutualistic interaction often potentiating the virulence of the individual members...
December 2016: Cellular Microbiology
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