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Mixed species biofilm

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https://www.readbyqxmd.com/read/29124888/differential-oxidative-stress-tolerance-of-streptococcus-mutans-isolates-affects-competition-in-an-ecological-mixed-species-biofilm-model
#1
Yuan Liu, Sara R Palmer, Hsiaochi Chang, Ashton N Combs, Robert A Burne, Hyun Koo
Streptococcus mutans strongly influences the development of pathogenic biofilms associated with dental caries. Our understanding of S. mutans behavior in biofilms is based on a few well-characterized laboratory strains; however, individual isolates vary widely in genome content and virulence-associated phenotypes, such as biofilm formation and environmental stress sensitivity. Using an ecological biofilm model, we assessed the impact of co-cultivation of several S. mutans isolates with Streptococcus oralis and Actinomyces naeslundii on biofilm composition following exposure to sucrose...
November 10, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/29123396/use-of-magnetic-nanoparticles-as-a-drug-delivery-system-to-improve-chlorhexidine-antimicrobial-activity
#2
Grażyna Tokajuk, Katarzyna Niemirowicz, Piotr Deptuła, Ewelina Piktel, Mateusz Cieśluk, Agnieszka Z Wilczewska, Jan R Dąbrowski, Robert Bucki
Nanotechnology offers new tools for developing therapies to prevent and treat oral infections, particularly biofilm-dependent disorders, such as dental plaques and endodontic and periodontal diseases. Chlorhexidine (CHX) is a well-characterized antiseptic agent used in dentistry with broad spectrum activity. However, its application is limited due to inactivation in body fluid and cytotoxicity toward human cells, particularly at high concentrations. To overcome these limitations, we synthesized nanosystems composed of aminosilane-coated magnetic nanoparticles functionalized with chlorhexidine (MNP@CHX)...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29111321/inhibition-activity-of-lactobacilli-supernatant-against-fungal-bacterial-multispecies-biofilms-on-silicone
#3
Yulong Tan, Matthias Leonhard, Doris Moser, Berit Schneider-Stickler
Fungal-bacterial multispecies biofilms play a major role in failure of medical silicone devices, such as voice prostheses in laryngectomiy. In this study, we determined the effect of Lactobacilli supernatant (cell free) on mixed biofilm formation of fungi and bacteria on silicone in vitro. Lactobacilli supernatant inhibited the adhesion (90 min) of mixed fungi and bacteria species with an efficiency of >90%. Mixed biofilm formation and the metabolic activity of the biofilms were inhibited by 72.23% and 58...
October 28, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29101060/dispersal-of-single-and-mixed-non-albicans-candida-species-biofilms-by-%C3%AE-1-3-glucanase-in-vitro
#4
Yulong Tan, Matthias Leonhard, Su Ma, Doris Moser, Berit Schneider-Stickler
β-1,3-glucan plays a role in non-albicans Candida species biofilm formation and survival of biofilm Candida to stresses. In this study, we evaluated the antibiofilm activity of β-1,3-glucanase, which can degrade poly-β(1 → 3)-glucose of non-albicans Candida species biofilms, on single and mixed species biofilm of non-albicans Candida species, including Candida tropicalis, Candida parapsilosis and Candida krusei. Biofilm by all tested species in microplate were dispersed more than 60%. β-1,3-glucanase also detached mixed species biofilm in microplate and on medical material surface...
November 1, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29099398/electric-field-based-dressing-disrupts-mixed-species-bacterial-biofilm-infection-and-restores-functional-wound-healing
#5
Kasturi Ganesh Barki, Amitava Das, Sriteja Dixith, Piya Das Ghatak, Shomita Mathew-Steiner, Elizabeth Schwab, Savita Khanna, Daniel J Wozniak, Sashwati Roy, Chandan K Sen
OBJECTIVE: This study was designed to employ electroceutical principles, as an alternative to pharmacological intervention, to manage wound biofilm infection. Mechanism of action of a United States Food and Drug Administration-cleared wireless electroceutical dressing (WED) was tested in an established porcine chronic wound polymicrobial biofilm infection model involving inoculation with Pseudomonas aeruginosa PAO1 and Acinetobacter baumannii 19606. BACKGROUND: Bacterial biofilms represent a major wound complication...
November 2, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/29079615/%C3%AE-glucanase-activity-of-the-oral-bacterium-tannerella-forsythia-contributes-to-the-growth-of-a-partner-species-fusobacterium-nucleatum-in-co-biofilms
#6
Kiyonobu Honma, Angela Ruscitto, Ashu Sharma
Tannerella forsythia and Fusobacterium nucleatum are dental plaque bacteria implicated in the development of periodontitis. These two species have been shown to form synergistic biofilms and found to be closely associated in dental plaque biofilms. A number of genetic loci for TonB-dependent membrane receptors (TDR) for glycan acquisition, with many existing in association with genes coding for enzymes involved in the breakdown of complex glycans, have been identified in T. forsythia In this study, we focused on a locus, BFO_0186 -- BFO_0188, that codes for a predicted TDR-SusD transporter along with a putative β-glucan hydrolyzing enzyme (BFO_0186)...
October 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29078172/conductive-properties-of-methanogenic-biofilms
#7
Cheng Li, Keaton Larson Lesnik, Hong Liu
Extracellular electron transfer between syntrophic partners needs to be efficiently maintained in methanogenic environments. Direct extracellular electron transfer via electrical current is an alternative to indirect hydrogen transfer but requires construction of conductive extracellular structures. Conductive mechanisms and relationship between conductivity and the community composition in mixed-species methanogenic biofilms are not well understood. The present study investigated conductive behaviors of methanogenic biofilms and examined the correlation between biofilm conductivity and community composition between different anaerobic biofilms enriched from the same inoculum...
October 19, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/29075322/pulse-electromagnetic-fields-enhance-extracellular-electron-transfer-in-magnetic-bioelectrochemical-systems
#8
Huihui Zhou, Bingfeng Liu, Qisong Wang, Jianmin Sun, Guojun Xie, Nanqi Ren, Zhiyong Jason Ren, Defeng Xing
BACKGROUND: Microbial extracellular electron transfer (EET) is essential in driving the microbial interspecies interaction and redox reactions in bioelectrochemical systems (BESs). Magnetite (Fe3O4) and magnetic fields (MFs) were recently reported to promote microbial EET, but the mechanisms of MFs stimulation of EET and current generation in BESs are not known. This study investigates the behavior of current generation and EET in a state-of-the-art pulse electromagnetic field (PEMF)-assisted magnetic BES (PEMF-MBES), which was equipped with magnetic carbon particle (Fe3O4@N-mC)-coated electrodes...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29031236/inhibitory-effect-of-probiotic-lactobacilli-supernatants-on-single-and-mixed-non-albicans-candida-species-biofilm
#9
Yulong Tan, Matthias Leonhard, Doris Moser, Su Ma, Berit Schneider-Stickler
OBJECTIVES: Oral candidiasis is one of the most common human fungal infections. While most cases of the Candida species isolated from the oral cavity are Candida albicans, a large number of candidiasis is attributed to non-albicans Candida species. In this study, we aim to evaluate the in vitro inhibition of supernatants of Lactobacillus gasseri and Lactobacillus rhamnosus on the single and mixed species biofilm of non-albicans Candida species, including Candida tropicalis, Candida krusei and Candida parapsilosis...
October 6, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29027368/expression-of-fluorescent-proteins-in-lactobacillus-rhamnosus-to-study-host-microbe-and-microbe-microbe-interactions
#10
Irina Spacova, Elke Lievens, Tine Verhoeven, Hans Steenackers, Jos Vanderleyden, Sarah Lebeer, Mariya I Petrova
Probiotic Lactobacillus strains are widely used to benefit human and animal health, although the exact mechanisms behind their interactions with the host and the microbiota are largely unknown. Fluorescent tagging of live probiotic cells is an important tool to unravel their modes of action. In this study, the implementation of different heterologously expressed fluorescent proteins for the labelling of the model probiotic strains Lactobacillus rhamnosusGG (gastrointestinal) and Lactobacillus rhamnosusGR-1 (vaginal) was explored...
October 13, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/29025667/adhesion-forces-of-biofilms-developed-in-vitro-from-clinical-strains-of-skin-wounds
#11
Elizabeth Alvarado-Gomez, Mario Perez-Diaz, Donato Valdez-Perez, Jaime Ruiz-Garcia, Martin Magaña-Aquino, Gabriel Martinez-Castañon, Fidel Martinez-Gutierrez
A biofilm is a very complex consortium formed by a mix of different microorganisms, which have become an important health problem, because its formation is a resistance mechanism used by bacteria against antibiotics or the immune system. In this work, we show differences between some physicochemical properties of biofilms in mono- and multi-species, formed by bacteria from clinical samples of infected chronic wounds. Of the most prevalent bacteria in wounds, two mono- and one multi-species biofilms were developed in vitro by Drip Flow Reactor: one biofilm was developed by S...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28987620/evaluation-of-culture-conditions-for-mixed-biofilm-formation-with-clinically-isolated-non-albicans-candida-species-and-staphylococcus-epidermidis-on-silicone
#12
Yulong Tan, Matthias Leonhard, Berit Schneider-Stickler
Silicone is frequently used in clinical and medical fields for medical devices. Mixed biofilms composed of Candida and bacterial species causes frequently failure of medical silicone devices, In this in vitro study, we analyzed mixed biofilm formation of clinically isolated non-albicans Candida species and Staphylococcus epidermidis, including Candida tropicalis, Candida krusei and Candida parapsilosis under the influence of different growth media (RPMI 1640, BHI and TSB) and several culture variables (incubation period, feeding conditions and FBS)...
October 5, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28966064/in%C3%A2-vitro-assessment-of-stainless-steel-orthodontic-brackets-coated-with-titanium-oxide-mixed-ag-for-anti-adherent-and-antibacterial-properties-against-streptococcus-mutans-and-porphyromonas-gingivalis
#13
Eman Jameel Fatani, Hamed H Almutairi, Ali O Alharbi, Yasser Obaidallah Alnakhli, Darshan Devang Divakar, Muzaheed, Abdulaziz Abdullah Alkheraif, Aftab Ahmed Khan
Orthodontic brackets made from stainless steel were introduced in dentistry, though they have less ability in reducing enamel demineralization and are not successful in preventing microbial as well as biofilm growth. In this study, we evaluated the significant role of different brackets in reducing enamel demineralization indirectly. Results from different tests indicate the significant reduction in adhesion, biofilm formation and slow growth of tested bacterial species on brackets coated with Ag + TiO2 and found to be statistically significant lower than control...
September 29, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28961860/effect-of-bacterial-components-of-mixed-culture-supernatants-of-planktonic-and-biofilm-pseudomonas-aeruginosa-with-commensal-escherichia-coli-on-the-neutrophil-response-in-vitro
#14
I L Maslennikova, M V Kuznetsova, I V Nekrasova, S V Shirshev
Pseudomonas aeruginosa (PA) responsible for acute and chronic infections often forms a well-organized bacterial population with different microbial species including commensal strains of Escherichia coli. Bacterial extracellular components of mixed culture can modulate the influence of bacteria on the neutrophil functions. The objective of this study was to compare the effect of pyocyanin, pyoverdine, LPS, exopolysaccharide of single species and mixed culture supernatants of PA strains and E. coli K12 on microbicidal, secretory activity of human neutrophils in vitro...
August 31, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28946780/extracellular-dna-and-lipoteichoic-acids-interact-with-exopolysaccharides-in-the-extracellular-matrix-of-streptococcus-mutans-biofilms
#15
Midian C Castillo Pedraza, Tatiana F Novais, Roberta C Faustoferri, Robert G Quivey, Anton Terekhov, Bruce R Hamaker, Marlise I Klein
Streptococcus mutans-derived exopolysaccharides are virulence determinants in the matrix of biofilms that cause caries. Extracellular DNA (eDNA) and lipoteichoic acid (LTA) are found in cariogenic biofilms, but their functions are unclear. Therefore, strains of S. mutans carrying single deletions that would modulate matrix components were used: eDNA - ∆lytS and ∆lytT; LTA - ∆dltA and ∆dltD; and insoluble exopolysaccharide - ΔgtfB. Single-species (parental strain S. mutans UA159 or individual mutant strains) and mixed-species (UA159 or mutant strain, Actinomyces naeslundii and Streptococcus gordonii) biofilms were evaluated...
September 25, 2017: Biofouling
https://www.readbyqxmd.com/read/28893777/modulation-of-staphylococcus-aureus-response-to-antimicrobials-by-the-candida-albicans-quorum-sensing-molecule-farnesol
#16
Eric F Kong, Christina Tsui, Sona Kucharíková, Patrick Van Dijck, Mary Ann Jabra-Rizk
In microbial biofilms, microorganisms utilize secreted signaling chemical molecules to coordinate their collective behavior. Farnesol is a quorum-sensing molecule secreted by the fungal species Candida albicans, shown to play a central physiological role during fungal biofilm growth. Our pervious in vitro and in vivo studies characterized an intricate interaction between C. albicans and the bacterial pathogen Staphylococcus aureus, as these species co-exist in biofilm. In this study, we aimed to investigate the impact of farnesol on S...
September 11, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28866443/subminimal-inhibitory-concentration-sub-mic-of-antibiotic-induces-electroactive-biofilm-formation-in-bioelectrochemical-systems
#17
Lean Zhou, Tian Li, Jingkun An, Chengmei Liao, Nan Li, Xin Wang
Electroactive biofilms (EABs) generated from mixed inocula are attractive due to their unique direct extracellular electron transfer abilities and potential use in water pollution control. In this study, for the first time, we identified a chemical that can be used for EAB regulation (both inhibition and promotion). We confirmed that tobramycin, an antibiotic previously demonstrated to inhibit the activity of EABs, is an agonist of EAB formation at subminimal inhibitory concentrations (sub-MICs). Compared to the control, at tobramycin concentrations of 0...
November 15, 2017: Water Research
https://www.readbyqxmd.com/read/28861053/enhanced-biocide-treatments-with-d-amino-acid-mixtures-against-a-biofilm-consortium-from-a-water-cooling-tower
#18
Ru Jia, Yingchao Li, Hussain H Al-Mahamedh, Tingyue Gu
Different species of microbes form mixed-culture biofilms in cooling water systems. They cause microbiologically influenced corrosion (MIC) and biofouling, leading to increased operational and maintenance costs. In this work, two D-amino acid mixtures were found to enhance two non-oxidizing biocides [tetrakis hydroxymethyl phosphonium sulfate (THPS) and NALCO 7330 (isothiazoline derivatives)] and one oxidizing biocide [bleach (NaClO)] against a biofilm consortium from a water cooling tower in lab tests. Fifty ppm (w/w) of an equimass mixture of D-methionine, D-leucine, D-tyrosine, D-tryptophan, D-serine, D-threonine, D-phenylalanine, and D-valine (D8) enhanced 15 ppm THPS and 15 ppm NALCO 7330 with similar efficacies achieved by the 30 ppm THPS alone treatment and the 30 ppm NALCO 7330 alone treatment, respectively in the single-batch 3-h biofilm removal test...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28823093/candida-albicans-staphylococcus-aureus-dual-species-biofilm-as-a-target-for-the-combination-of-essential-oils-and-fluconazole-or-mupirocin
#19
Aleksandra Budzyńska, Sylwia Różalska, Beata Sadowska, Barbara Różalska
The effectiveness of essential oils (EOs), fluconazole (FLU) and mupirocin (MUP) used alone or in combination against mono-species and mixed Candida albicans/Staphylococcus aureus biofilms was examined. An experimentally established dual-species biofilm model, verified by fluorescence microscopy and viable cell counting, was used. Selected commercial EOs were tested: geranium, citronella and clove oils, which have been chemically characterized and found to differ in the content of the main components (qualitative and quantitative)...
August 19, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28812881/predicting-microbial-fuel-cell-biofilm-communities-and-bioreactor-performance-using-artificial-neural-networks
#20
Keaton Larson Lesnik, Hong Liu
The complex interactions that occur in mixed-species bioelectrochemical reactors, like microbial fuel cells (MFCs), make accurate predictions of performance outcomes under untested conditions difficult. While direct correlations between any individual waste stream characteristic or microbial community structure and reactor performance have not been able to be directly established, the increase in sequencing data and readily available computational power enables the development of alternate approaches. In the current study, 33 MFCs were evaluated under a range of conditions including eight separate substrates and three different wastewaters...
August 25, 2017: Environmental Science & Technology
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