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Mixed species biofilm

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https://www.readbyqxmd.com/read/28625992/flow-cytometry-a-powerful-novel-tool-to-assess-viability-of-fungal-conidia-in-metal-working-fluids
#1
D Vanhauteghem, K Demeyere, N Callaert, A Boelaert, G Haesaert, K Audenaert, E Meyer
Fungal contamination of metal working fluids (MWF) is a dual problem in automatized processing plants because resulting fungal biofilms obstruct cutting, drilling and polishing machines. Moreover, some fungal species of MWF comprise pathogens such as Fusarium solani Therefore, the development of an accurate analytical tool to evaluate conidia viability in MWF is important. We developed a flow cytometric method to measure fungal viability in MWF using F. solani as model organism. To validate this method, viable and dead conidia were mixed in several proportions and flow cytometrically analyzed...
June 16, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28624271/coagulase-negative-staphylococci-species-affect-biofilm-formation-of-other-coagulase-negative-and-coagulase-positive-staphylococci
#2
Coralie Goetz, Yannick D N Tremblay, Daphnée Lamarche, Andréanne Blondeau, Annie M Gaudreau, Josée Labrie, François Malouin, Mario Jacques
Coagulase-negative staphylococci (CNS) are considered to be commensal bacteria in humans and animals, but are now also recognized as etiological agents in several infections, including bovine mastitis. Biofilm formation appears to be an important factor in CNS pathogenicity. Furthermore, some researchers have proposed that CNS colonization of the intramammary environment has a protective effect against other pathogens. The mechanisms behind the protective effect of CNS have yet to be characterized. The aim of this study was to evaluate the effect of CNS isolates with a weak-biofilm phenotype on the biofilm formation of other staphylococcal isolates...
June 14, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28622460/antifungal-activity-of-tyrosol-and-farnesol-used-in-combination-against-candida-species-in-the-planktonic-state-or-forming-biofilms
#3
Douglas Roberto Monteiro, Laís Salomão Arias, Renan Aparecido Fernandes, Lucas Fernando Deszo da Silva, Manoel Olavo Valentim Fernandes de Castilho, Thaís Oliveira da Rosa, Ana Paula Miranda Vieira, Fabiana Gouveia Straioto, Débora Barros Barbosa, Alberto Carlos Botazzo Delbem
AIM: This study examined the antifungal activity of the combination of tyrosol and farnesol against Candida albicans and Candida glabrata in the planktonic state or forming biofilms. METHODS AND RESULTS: The effect of drug association against Candida planktonic cells was assessed by the fractional inhibitory concentration index. Mono and dual-species biofilms were developed (24 h) and the compounds, applied during 3 days, with 2 daily treatments of 1 minute each...
June 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28617874/candida-albicans-mannans-mediate-streptococcus-mutans-exoenzyme-gtfb-binding-to-modulate-cross-kingdom-biofilm-development-in-vivo
#4
Geelsu Hwang, Yuan Liu, Dongyeop Kim, Yong Li, Damian J Krysan, Hyun Koo
Candida albicans is frequently detected with heavy infection by Streptococcus mutans in plaque-biofilms from children with early-childhood caries (ECC). This cross-kingdom biofilm contains an extensive matrix of extracellular α-glucans that is produced by an exoenzyme (GtfB) secreted by S. mutans. Here, we report that mannans located on the outer surface of C. albicans cell-wall mediates GtfB binding, enhancing glucan-matrix production and modulating bacterial-fungal association within biofilms formed in vivo...
June 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28605597/bacterial-gtfb-augments-candida-albicans-accumulation-in-cross-kingdom-biofilms
#5
K Ellepola, Y Liu, T Cao, H Koo, C J Seneviratne
Streptococcus mutans is a biofilm-forming oral pathogen commonly associated with dental caries. Clinical studies have shown that S. mutans is often detected with Candida albicans in early childhood caries. Although the C. albicans presence has been shown to enhance bacterial accumulation in biofilms, the influence of S. mutans on fungal biology in this mixed-species relationship remains largely uncharacterized. Therefore, we aimed to investigate how the presence of S. mutans influences C. albicans biofilm development and coexistence...
June 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28596759/bsocial-deciphering-social-behaviors-within-mixed-microbial-populations
#6
Jessica Purswani, Rocío C Romero-Zaliz, Antonio M Martín-Platero, Isabel M Guisado, Jesús González-López, Clementina Pozo
Ecosystem functionality depends on interactions among populations, of the same or different taxa, and these are not just the sum of pairwise interactions. Thus, know-how of the social interactions occurring in mixed-populations are of high interest, however they are commonly unknown due to the limitations posed in tagging each population. The limitations include costs/time in tediously fluorescent tagging, and the number of different fluorescent tags. Tag-free strategies exist, such as high-throughput sequencing, but ultimately both strategies require the use of expensive machinery...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28554609/biofilm-induced-changes-to-the-composite-surface
#7
Ivana Nedeljkovic, Jan De Munck, Andreea-Alexandra Ungureanu, Vera Slomka, Carmen Bartic, Anja Vananroye, Christian Clasen, Wim Teughels, Bart Van Meerbeek, Kirsten L Van Landuyt
OBJECTIVES: Composites may undergo biodegradation in the oral cavity. The objective was to investigate the effect of single- and multi-species biofilms on the surface roughness and topography of two composites. METHODS: Disk-shaped specimens of a paste-like, Bis-GMA-free (Gradia Direct Anterior, GC), and a flowable, Bis-GMA-based composite (Tetric EvoFlow, Ivoclar-Vivadent) were prepared. After ethylene-oxide sterilization (38°C), specimens (n=3) were incubated with Streptococcus mutans or mixed bacterial culture (Streptococcus mutans, Streptococcus sanguinis, Actinomyces naeslundii and Fusobacterium nucleatum)...
May 26, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28526497/mixed-species-biofilms-of-fusobacterium-necrophorum-and-porphyromonas-levii-impair-the-oxidative-response-of-bovine-neutrophils-in%C3%A2-vitro
#8
Joey S Lockhart, Andre G Buret, Howard Ceri, Douglas G Storey, Stefanie J Anderson, Douglas W Morck
Biofilms composed of anaerobic bacteria can result in persistent infections and chronic inflammation. Host immune cells have difficulties clearing biofilm-related infections and this can result in tissue damage. Neutrophils are a vital component of the innate immune system and help clear biofilms. The comparative neutrophilic response to biofilms versus planktonic bacteria remains incompletely understood, particularly in the context of mixed infections. The objective of this study was to generate mixed species anaerobic bacterial biofilms composed of two opportunistic pathogens, Fusobacterium necrophorum and Porphyromonas levii, and evaluate neutrophil responses to extracellular fractions from both biofilms and planktonic cell co-cultures of the same bacteria...
May 17, 2017: Anaerobe
https://www.readbyqxmd.com/read/28502779/microeukaryote-community-in-a-partial-nitritation-reactor-prior-to-anammox-and-an-insight-into-the-potential-of-ciliates-as-performance-bioindicators
#9
Oriol Canals, Ramon Massana, Joan Lluís Riera, Vanessa Balagué, Humbert Salvadó
An in-depth, long-term, multidisciplinary study was conducted in order to study the microeukaryote community in a partial nitritation (PN) reactor prior to anammox. The PN reactor operated with moving bed biofilm reactor (MBBR) technology, using plastic supports (carriers) for biofilm development. The microeukaryote community from the biofilm (BF) and the surrounding media (mixed liquor or ML) were analysed separately. Despite the physicochemical conditions under which the PN-MBBR operated (an average of 305...
May 11, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28485524/streptococcus-gordonii-luxs-autoinducer-2-quorum-sensing-system-modulates-the-dual-species-biofilm-formation-with-streptococcus-mutans
#10
Xiao Wang, Xiaolan Li, Junqi Ling
Dental plaques are mixed-species biofilms that are related to the development of dental caries. Streptococcus mutans (S. mutans) is an important cariogenic bacterium that forms mixed-species biofilms with Streptococcus gordonii (S. gordonii), an early colonizer of the tooth surface. The LuxS/autoinducer-2(AI-2) quorum sensing system is involved in the regulation of mixed-species biofilms, and AI-2 is proposed as a universal signal for the interaction between bacterial species. In this work, a S. gordonii luxS deficient strain was constructed to investigate the effect of the S...
May 9, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28470309/characterization-of-non-dialyzable-constituents-from-cranberry-juice-that-inhibit-adhesion-co-aggregation-and-biofilm-formation-by-oral-bacteria
#11
C C Neto, K A Penndorf, M Feldman, S Meron-Sudai, Z Zakay-Rones, D Steinberg, M Fridman, Y Kashman, I Ginsburg, I Ofek, E I Weiss
An extract prepared from cranberry juice by dialysis known as nondialyzable material (NDM) has been shown previously to possess anti-adhesion activity toward microbial species including oral bacteria, uropathogenic Escherichia coli and Helicobacter pylori. Bioassay-guided fractionation of cranberry NDM was therefore undertaken to identify the anti-adhesive constituents. An aqueous acetone-soluble fraction (NDMac) obtained from Sephadex LH-20 inhibited adhesion-linked activities by oral bacteria, including co-aggregation of oral bacteria Fusobacterium nucleatum with Streptococcus sanguinis or Porphyromonas gingivalis, and biofilm formation by Streptococcus mutans...
May 24, 2017: Food & Function
https://www.readbyqxmd.com/read/28461319/bacteriophage-lysin-cf-301-a-potent-anti-staphylococcal-biofilm-agent
#12
Raymond Schuch, Babar K Khan, Assaf Raz, Jimmy A Rotolo, Michael Wittekind
Biofilms pose a unique therapeutic challenge because of the antibiotic tolerance of constituent bacteria. Treatments for biofilm-based infections represent a major unmet medical need, requiring novel agents to eradicate mature biofilms. Our objective was to evaluate bacteriophage lysin CF-301 as a new agent to target Staphylococcus aureus biofilms. We used minimum biofilm eradicating concentration (MBEC) assays on ninety-five S. aureus strains to obtain a MBEC90 value of ≤0.25 μg/ml for CF-301. Mature biofilms of coagulase-negative staphylococci, Streptococcus pyogenes, and Streptococcus agalactiae were also sensitive to disruption, with MBEC90 values ranging from 0...
May 1, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28414441/suppression-of-enteric-bacteria-by-bacteriophages-importance-of-phage-polyvalence-in-the-presence-of-soil-bacteria
#13
Pingfeng Yu, Jacques Mathieu, Yu Yang, Pedro J J Alvarez
Bacteriophages are widely recognized for their importance in microbial ecology and bacterial control. However, little is known about how phage polyvalence (i.e., broad host range) affects bacterial suppression and interspecies competition in environments harboring enteric pathogens and soil bacteria. Here we compare the efficacy of polyvalent phage PEf1 versus coliphage T4 in suppressing a model enteric bacterium (E. coli K-12) in mixtures with soil bacteria (Pseudomonas putida F1 and Bacillus subtilis 168)...
May 2, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28409363/advances-in-the-treatment-of-problematic-industrial-biofilms
#14
REVIEW
D Xu, R Jia, Y Li, T Gu
In nature, microorganisms tend to form biofilms that consist of extracellular polymeric substances with embedded sessile cells. Biofilms, especially mixed-culture synergistic biofilm consortia, are notoriously difficult to treat. They employ various defense mechanisms against attacks from antimicrobial agents. Problematic industrial biofilms cause biofouling as well as biocorrosion, also known as microbiologically influenced corrosion. Biocides are often used to treat biofilms together with scrubbing or pigging...
May 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28403648/consumption-of-apple-boysenberry-beverage-decreases-salivary-actinomyces-naeslundii-and-their-adhesion-in-a-multi-species-biofilm-model
#15
S G Parkar, S Eady, M Cabecinha, M A Skinner
We hypothesised that consumption of beverage rich in both fibre and polyphenols, rather than each bioactive alone, will modulate populations of selected salivary bacteria, and their adhesion characteristics and that some of these effects may be due to the anti-microbial activity of the beverage bioactives. We investigated the effect of 4 weeks' consumption of beverages, rich in apple fibre, boysenberry polyphenols, or both on salivary bacteria in healthy subjects. In this placebo-controlled crossover study, saliva samples were collected at the beginning and end of each treatment period, and used for qPCR quantitation of Lactobacillus spp...
April 26, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28401747/dynamics-of-mono-and-dual-species-biofilm-formation-and-interactions-between-staphylococcus-aureus-and-gram-negative-bacteria
#16
Jitka Makovcova, Vladimir Babak, Pavel Kulich, Josef Masek, Michal Slany, Lenka Cincarova
Microorganisms are not commonly found in the planktonic state but predominantly form dual- and multispecies biofilms in almost all natural environments. Bacteria in multispecies biofilms cooperate, compete or have neutral interactions according to the involved species. Here, the development of mono- and dual-species biofilms formed by Staphylococcus aureus and other foodborne pathogens such as Salmonella enterica subsp. enterica serovar Enteritidis, potentially pathogenic Raoultella planticola and non-pathogenic Escherichia coli over the course of 24, 48 and 72 h was studied...
April 11, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28390465/antibiofilm-activity-of-five-different-endodontic-filling-materials-used-in-primary-teeth-using-confocal-laser-scanning-microscopy
#17
Katerine Jahnecke Pilownic, Ceci Nunes Carvalho, Ana Regina Romano, Renata Dornelles Morgental, Ya Shen, Markus Haapasalo, Fernanda Geraldo Pappen
PURPOSE: The purpose of this study was to evaluate the effect of five endodontic materials for primary teeth on in vitro mixed-species biofilms. METHODS: Zinc oxide eugenol (ZOE) cement, Vitapex, Calen paste thickened with zinc oxide (ZO), pure calcium hydroxide (Ca(OH)2) paste, and iodoform were evaluated. Sterile water was used as a control. Mixed-species biofilm was incubated in anaerobic conditions for 21 days. The biofilm specimens were placed in contact with the endodontic materials for periods of seven and 30 days...
March 15, 2017: Pediatric Dentistry
https://www.readbyqxmd.com/read/28382026/interstrain-cooperation-in-meningococcal-biofilms-role-of-autotransporters-nalp-and-auta
#18
Jesús Pérez-Ortega, Antonio Rodríguez, Eduardo Ribes, Jan Tommassen, Jesús Arenas
Neisseria meningitidis (Nm) and Neisseria lactamica (Nl) are commensal bacteria that live in the human nasopharynx, where they form microcolonies. In contrast to Nl, Nm occasionally causes blood and/or meningitis infection with often fatal consequences. Here, we studied interactions between neisserial strains during biofilm formation. Fluorescent strains were engineered and analyzed for growth in single- and dual-strain biofilms with confocal laser-scanning microscopy. Different strains of diverse Neisseria species formed microcolonies of different sizes and morphologies...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28380676/bactericidal-effect-of-photocatalytically-active-nanostructured-tio2-surfaces-on-biofilms-of-the-early-oral-colonizer-streptococcus-oralis
#19
Emma Westas, Mariko Hayashi, Francesca Cecchinato, Ann Wennerberg, Martin Andersson, Ryo Jimbo, Julia R Davies
This study evaluated the photocatalytic bactericidal effect of nanostructured anatase-rich titanium dioxide (TiO2 ) on biofilms. Commercially pure titanium discs were spin-coated with photocatalytic TiO2 nanoparticles (P25). Uncoated discs were used as control (CTRL). Half of the CTRL and half of the P25-coated surfaces were coated with purified saliva (SAL) to give four different groups (CTRL, CTRL+SAL, P25 and P25+SAL). Streptococcus oralis were allowed to form biofilms on the discs for 18 hours and non-adherent cells were rinsed off thereafter...
April 5, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28337568/hospital-microbial-surface-colonization-revealed-during-monitoring-of-klebsiella-spp-pseudomonas-aeruginosa-and-non-tuberculous-mycobacteria
#20
Pedro Geadas Farias, Fernando Gama, Diogo Reis, Susana Alarico, Nuno Empadinhas, José Carlos Martins, Ana Figueiredo de Almeida, Paula Vasconcelos Morais
Hospital environmental conditions, human occupancy, and the characteristics of the equipment influence the survival of microbial communities and raise a concern with regard to nosocomial infections. The objective of the present work was to use the monitoring of Pseudomonas aeruginosa, Klebsiella spp. and non-tuberculous mycobacteria as a strategy to improve knowledge on microbial colonization of non-critical equipment and surfaces, in a tertiary hospital from Central Portugal. A 3-month microbiological survey was performed in a district teaching hospital...
March 23, 2017: Antonie Van Leeuwenhoek
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