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Mixed species biofilm

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https://www.readbyqxmd.com/read/28526497/mixed-species-biofilms-of-fusobacterium-necrophorum-and-porphyromonas-levii-impair-the-oxidative-response-of-bovine-neutrophils-in-vitro
#1
Joey S Lockhart, Andre G Buret, Howard Ceri, Douglas G Storey, Stefanie J Anderson, Douglas W Morck
Biofilms composed of anaerobic bacteria can result in persistent infections and chronic inflammation. Host immune cells have difficulties clearing biofilm-related infections and this can result in tissue damage. Neutrophils are a vital component of the innate immune system and help clear biofilms. The comparative neutrophilic response to biofilms versus planktonic bacteria remains incompletely understood, particularly in the context of mixed infections. The objective of this study was to generate mixed species anaerobic bacterial biofilms composed of two opportunistic pathogens, Fusobacterium necrophorum and Porphyromonas levii, and evaluate neutrophil responses to extracellular fractions from both biofilms and planktonic cell co-cultures of the same bacteria...
May 16, 2017: Anaerobe
https://www.readbyqxmd.com/read/28502779/microeukaryote-community-in-a-partial-nitritation-reactor-prior-to-anammox-and-an-insight-into-the-potential-of-ciliates-as-performance-bioindicators
#2
Oriol Canals, Ramon Massana, Joan Lluís Riera, Vanessa Balagué, Humbert Salvadó
An in-depth, long-term, multidisciplinary study was conducted in order to study the microeukaryote community in a partial nitritation (PN) reactor prior to anammox. The PN reactor operated with moving bed biofilm reactor (MBBR) technology, using plastic supports (carriers) for biofilm development. The microeukaryote community from the biofilm (BF) and the surrounding media (mixed liquor or ML) were analysed separately. Despite the physicochemical conditions under which the PN-MBBR operated (an average of 305...
May 11, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28485524/streptococcus-gordonii-luxs-autoinducer-2-quorum-sensing-system-modulates-the-dual-species-biofilm-formation-with-streptococcus-mutans
#3
Xiao Wang, Xiaolan Li, Junqi Ling
Dental plaques are mixed-species biofilms that are related to the development of dental caries. Streptococcus mutans (S. mutans) is an important cariogenic bacterium that forms mixed-species biofilms with Streptococcus gordonii (S. gordonii), an early colonizer of the tooth surface. The LuxS/autoinducer-2(AI-2) quorum sensing system is involved in the regulation of mixed-species biofilms, and AI-2 is proposed as a universal signal for the interaction between bacterial species. In this work, a S. gordonii luxS deficient strain was constructed to investigate the effect of the S...
May 9, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28470309/characterization-of-non-dialyzable-constituents-from-cranberry-juice-that-inhibit-adhesion-co-aggregation-and-biofilm-formation-by-oral-bacteria
#4
C C Neto, K A Penndorf, M Feldman, S Meron-Sudai, Z Zakay-Rones, D Steinberg, M Fridman, Y Kashman, I Ginsburg, I Ofek, E I Weiss
An extract prepared from cranberry juice by dialysis known as nondialyzable material (NDM) has been shown previously to possess anti-adhesion activity toward microbial species including oral bacteria, uropathogenic Escherichia coli and Helicobacter pylori. Bioassay-guided fractionation of cranberry NDM was therefore undertaken to identify the anti-adhesive constituents. An aqueous acetone-soluble fraction (NDMac) obtained from Sephadex LH-20 inhibited adhesion-linked activities by oral bacteria, including co-aggregation of oral bacteria Fusobacterium nucleatum with Streptococcus sanguinis or Porphyromonas gingivalis, and biofilm formation by Streptococcus mutans...
May 4, 2017: Food & Function
https://www.readbyqxmd.com/read/28461319/bacteriophage-lysin-cf-301-a-potent-anti-staphylococcal-biofilm-agent
#5
Raymond Schuch, Babar K Khan, Assaf Raz, Jimmy A Rotolo, Michael Wittekind
Biofilms pose a unique therapeutic challenge because of the antibiotic tolerance of constituent bacteria. Treatments for biofilm-based infections represent a major unmet medical need, requiring novel agents to eradicate mature biofilms. Our objective was to evaluate bacteriophage lysin CF-301 as a new agent to target Staphylococcus aureus biofilms. We used minimum biofilm eradicating concentration (MBEC) assays on ninety-five S. aureus strains to obtain a MBEC90 value of ≤0.25 μg/ml for CF-301. Mature biofilms of coagulase-negative staphylococci, Streptococcus pyogenes, and Streptococcus agalactiae were also sensitive to disruption, with MBEC90 values ranging from 0...
May 1, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28414441/suppression-of-enteric-bacteria-by-bacteriophages-importance-of-phage-polyvalence-in-the-presence-of-soil-bacteria
#6
Pingfeng Yu, Jacques Mathieu, Yu Yang, Pedro J J Alvarez
Bacteriophages are widely recognized for their importance in microbial ecology and bacterial control. However, little is known about how phage polyvalence (i.e., broad host range) affects bacterial suppression and interspecies competition in environments harboring enteric pathogens and soil bacteria. Here we compare the efficacy of polyvalent phage PEf1 versus coliphage T4 in suppressing a model enteric bacterium (E. coli K-12) in mixtures with soil bacteria (Pseudomonas putida F1 and Bacillus subtilis 168)...
April 21, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28409363/advances-in-the-treatment-of-problematic-industrial-biofilms
#7
REVIEW
D Xu, R Jia, Y Li, T Gu
In nature, microorganisms tend to form biofilms that consist of extracellular polymeric substances with embedded sessile cells. Biofilms, especially mixed-culture synergistic biofilm consortia, are notoriously difficult to treat. They employ various defense mechanisms against attacks from antimicrobial agents. Problematic industrial biofilms cause biofouling as well as biocorrosion, also known as microbiologically influenced corrosion. Biocides are often used to treat biofilms together with scrubbing or pigging...
May 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28403648/consumption-of-apple-boysenberry-beverage-decreases-salivary-actinomyces-naeslundii-and-their-adhesion-in-a-multi-species-biofilm-model
#8
S G Parkar, S Eady, M Cabecinha, M A Skinner
We hypothesised that consumption of beverage rich in both fibre and polyphenols, rather than each bioactive alone, will modulate populations of selected salivary bacteria, and their adhesion characteristics and that some of these effects may be due to the anti-microbial activity of the beverage bioactives. We investigated the effect of 4 weeks' consumption of beverages, rich in apple fibre, boysenberry polyphenols, or both on salivary bacteria in healthy subjects. In this placebo-controlled crossover study, saliva samples were collected at the beginning and end of each treatment period, and used for qPCR quantitation of Lactobacillus spp...
April 26, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28401747/dynamics-of-mono-and-dual-species-biofilm-formation-and-interactions-between-staphylococcus-aureus-and-gram-negative-bacteria
#9
Jitka Makovcova, Vladimir Babak, Pavel Kulich, Josef Masek, Michal Slany, Lenka Cincarova
Microorganisms are not commonly found in the planktonic state but predominantly form dual- and multispecies biofilms in almost all natural environments. Bacteria in multispecies biofilms cooperate, compete or have neutral interactions according to the involved species. Here, the development of mono- and dual-species biofilms formed by Staphylococcus aureus and other foodborne pathogens such as Salmonella enterica subsp. enterica serovar Enteritidis, potentially pathogenic Raoultella planticola and non-pathogenic Escherichia coli over the course of 24, 48 and 72 h was studied...
April 11, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28390465/antibiofilm-activity-of-five-different-endodontic-filling-materials-used-in-primary-teeth-using-confocal-laser-scanning-microscopy
#10
Katerine Jahnecke Pilownic, Ceci Nunes Carvalho, Ana Regina Romano, Renata Dornelles Morgental, Ya Shen, Markus Haapasalo, Fernanda Geraldo Pappen
PURPOSE: The purpose of this study was to evaluate the effect of five endodontic materials for primary teeth on in vitro mixed-species biofilms. METHODS: Zinc oxide eugenol (ZOE) cement, Vitapex, Calen paste thickened with zinc oxide (ZO), pure calcium hydroxide (Ca(OH)2) paste, and iodoform were evaluated. Sterile water was used as a control. Mixed-species biofilm was incubated in anaerobic conditions for 21 days. The biofilm specimens were placed in contact with the endodontic materials for periods of seven and 30 days...
March 15, 2017: Pediatric Dentistry
https://www.readbyqxmd.com/read/28382026/interstrain-cooperation-in-meningococcal-biofilms-role-of-autotransporters-nalp-and-auta
#11
Jesús Pérez-Ortega, Antonio Rodríguez, Eduardo Ribes, Jan Tommassen, Jesús Arenas
Neisseria meningitidis (Nm) and Neisseria lactamica (Nl) are commensal bacteria that live in the human nasopharynx, where they form microcolonies. In contrast to Nl, Nm occasionally causes blood and/or meningitis infection with often fatal consequences. Here, we studied interactions between neisserial strains during biofilm formation. Fluorescent strains were engineered and analyzed for growth in single- and dual-strain biofilms with confocal laser-scanning microscopy. Different strains of diverse Neisseria species formed microcolonies of different sizes and morphologies...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28380676/bactericidal-effect-of-photocatalytically-active-nanostructured-tio2-surfaces-on-biofilms-of-the-early-oral-colonizer-streptococcus-oralis
#12
Emma Westas, Mariko Hayashi, Francesca Cecchinato, Ann Wennerberg, Martin Andersson, Ryo Jimbo, Julia R Davies
This study evaluated the photocatalytic bactericidal effect of nanostructured anatase-rich titanium dioxide (TiO2 ) on biofilms. Commercially pure titanium discs were spin-coated with photocatalytic TiO2 nanoparticles (P25). Uncoated discs were used as control (CTRL). Half of the CTRL and half of the P25-coated surfaces were coated with purified saliva (SAL) to give four different groups (CTRL, CTRL+SAL, P25 and P25+SAL). Streptococcus oralis were allowed to form biofilms on the discs for 18 hours and non-adherent cells were rinsed off thereafter...
April 5, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28337568/hospital-microbial-surface-colonization-revealed-during-monitoring-of-klebsiella-spp-pseudomonas-aeruginosa-and-non-tuberculous-mycobacteria
#13
Pedro Geadas Farias, Fernando Gama, Diogo Reis, Susana Alarico, Nuno Empadinhas, José Carlos Martins, Ana Figueiredo de Almeida, Paula Vasconcelos Morais
Hospital environmental conditions, human occupancy, and the characteristics of the equipment influence the survival of microbial communities and raise a concern with regard to nosocomial infections. The objective of the present work was to use the monitoring of Pseudomonas aeruginosa, Klebsiella spp. and non-tuberculous mycobacteria as a strategy to improve knowledge on microbial colonization of non-critical equipment and surfaces, in a tertiary hospital from Central Portugal. A 3-month microbiological survey was performed in a district teaching hospital...
March 23, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28335189/synergistic-antibacterial-effects-of-nanoparticles-encapsulated-with-scutellaria-baicalensis-and-pure-chlorhexidine-on-oral-bacterial-biofilms
#14
Ken Cham-Fai Leung, Chaminda Jayampath Seneviratne, Xuan Li, Ping Chung Leung, Clara Bik San Lau, Chi-Hin Wong, Ka Yan Pang, Chun Wai Wong, Elaine Wat, Lijian Jin
Scutellariabaicalensis (SB) is a traditional Chinese medicine for treating infectious and inflammatory diseases. Our recent study shows potent antibacterial effects of nanoparticle-encapsulated chlorhexidine (Nano-CHX). Herein, we explored the synergistic effects of the nanoparticle-encapsulated SB (Nano-SB) and Nano-CHX on oral bacterial biofilms. Loading efficiency of Nano-SB was determined by thermogravimetric analysis, and its releasing profile was assessed by high-performance liquid chromatographyusing baicalin (a flavonoid compound of SB) as the marker...
April 7, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28322439/modeling-multidimensional-and-multispecies-biofilms-in-porous-media
#15
Youneng Tang, Haihu Liu
Modeling multidimensional and multispecies biofilm in porous media at the pore scale is challenging due to the need to simultaneously track the microbial community in the biofilms and the interfaces between the biofilms and the fluid. Therefore, researchers usually assume that the model has only one dimension in space or has only one microbial species. This work uses bioremediation of U(VI)-contaminated groundwater as the context to develop a two-dimensional and multispecies biofilm model. The model simulates the transverse mixing zone in which U(VI) is mixed with propionate, a nutrient externally supplied to stimulate the growth of microorganisms...
March 21, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28316949/evaluation-of-the-role-of-probiotics-in-endodontic-treatment-a-preliminary-study
#16
Aarti Bohora, Sharad Kokate
AIMS AND OBJECTIVES: The principal goal of endodontics is the prevention of periapical infection. Acute and chronic apical periodontitis occur due to the persistence of pathogenic microorganisms such as Enterococcus faecalis and Candida albicans harboring the root canal systems of the teeth. The concept of the use of probiotics in addressing endodontic disease is new and has not been studied adequately. On the basis of the success of probiotics in periodontal treatment, this preliminary work was performed (a) to evaluate the antibacterial efficacy of probiotics against common endodontic pathogens, i...
January 2017: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/28198976/role-of-candida-species-from-hiv-infected-children-in-enamel-caries-lesions-an-in-vitro-study
#17
Senda Charone, Maristela Barbosa Portela, Karol de Oliveira Martins, Rosangela Maria Soares, Gloria Fernanda Castro
Objectives: This study analyzed the capacity of Candida spp. from dental biofilm of HIV infected (HIV+) children to demineralize primary molar enamel in vitro by Transversal Microhardness (TMH), Polarized Light Microscopy (PLM) and the quantity of calcium ions (Ca2+) released from the enamel. Material and Methods: Candida spp. samples were isolated from the supragingival biofilm of HIV+ children. A hundred and forty (140) enamel blocks were randomly assigned to six groups: biofilm formed by C...
January 2017: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/28194955/luminometric-label-array-for-counting-and-differentiation-of-bacteria
#18
Milla Högmander, Catherine J Paul, Sandy Chan, Elina Hokkanen, Ville Eskonen, Tapio Pahikkala, Sari Pihlasalo
Methods for simple and fast detection and differentiation of bacterial species are required, for instance, in medicine, water quality monitoring, and the food industry. Here, we have developed a novel label array method for the counting and differentiation of bacterial species. This method is based on the nonspecific interactions of multiple unstable lanthanide chelates and selected chemicals within the sample leading to a luminescence signal profile that is unique to the bacterial species. It is simple, cost-effective, and/or user-friendly compared to many existing methods, such as plate counts on selective media, automatic (hemocytometer-based) cell counters, flow cytometry, and polymerase chain reaction (PCR)-based methods for identification...
February 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28164244/candida-krusei-and-candida-glabrata-reduce-the-filamentation-of-candida-albicans-by-downregulating-expression-of-hwp1-gene
#19
Patrícia Pimentel de Barros, Fernanda Freire, Rodnei Dennis Rossoni, Juliana Campos Junqueira, Antonio Olavo Cardoso Jorge
Pathogenicity of Candida albicans is associated with its capacity switch from yeast-like to hyphal growth. The hyphal form is capable to penetrate the epithelial surfaces and to damage the host tissues. Therefore, many investigations have focused on mechanisms that control the morphological transitions of C. albicans. Recently, certain studies have showed that non-albicans Candida species can reduce the capacity of C. albicans to form biofilms and to develop candidiasis in animal models. Then, the objective of this study was to evaluate the effects of Candida krusei and Candida glabrata on the morphogenesis of C...
February 6, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28161488/cold-atmospheric-pressure-plasma-elimination-of-clinically-important-single-and-mixed-species-biofilms
#20
Martina Modic, Neil P McLeod, J Mark Sutton, James L Walsh
Mixed-species biofilms reflect the natural environment of many pathogens in clinical settings and are highly resistant to disinfection methods. An indirect cold atmospheric-pressure air-plasma system was evaluated under two different discharge conditions for its ability to kill representative Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) pathogens. Plasma treatment of individual 24-h-old biofilms and mixed-species biofilms that contained additional species (Enterococcus faecalis and Klebsiella pneumoniae) was considered...
March 2017: International Journal of Antimicrobial Agents
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