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Mixed species biofilm

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https://www.readbyqxmd.com/read/29778067/succession-of-bacterial-and-fungal-communities-within-biofilms-of-a-chlorinated-drinking-water-distribution-system
#1
I Douterelo, K E Fish, J B Boxall
Understanding the temporal dynamics of multi-species biofilms in Drinking Water Distribution Systems (DWDS) is essential to ensure safe, high quality water reaches consumers after it passes through these high surface area reactors. This research studied the succession characteristics of fungal and bacterial communities under controlled environmental conditions fully representative of operational DWDS. Microbial communities were observed to increase in complexity after one month of biofilm development but they did not reach stability after three months...
May 3, 2018: Water Research
https://www.readbyqxmd.com/read/29768730/biofilm-in-voice-prosthesis-a-prospective-cohort-study-and-laboratory-tests-using-sonication-and-sem-analysis
#2
Jacopo Galli, Lea Calo', Duino Meucci, Monica Giuliani, Daniela Lucidi, Gaetano Paludetti, Riccardo Torelli, Maurizio Sanguinetti, Claudio Parrilla
OBJECTIVE: The objective of the study was to compare the biofilm growing pattern and its morphological extent on silicone and a teflon-like material using a sonication process and a Scanning Electron Microscope (SEM). DESIGN: A prospective cohort study and a laboratory study. SETTING: Otolaryngology -Head and Neck surgery Department and the Microbiology Institute. PARTICIPANTS: The participants included fifteen laryngectomized patients with phonatory prostheses, which were removed due to device failure, and two different kinds of phonatory prostheses from the laboratory (Provox 2 and ActiValve) that were artificially colonized by Candida albicans...
May 16, 2018: Clinical Otolaryngology
https://www.readbyqxmd.com/read/29746596/the-hyr1-protein-from-the-fungus-candida-albicans-is-a-cross-kingdom-immunotherapeutic-target-for-acinetobacter-bacterial-infection
#3
Priya Uppuluri, Lin Lin, Abdullah Alqarihi, Guanpingsheng Luo, Eman G Youssef, Sondus Alkhazraji, Nannette Y Yount, Belal A Ibrahim, Michael Anthony Bolaris, John E Edwards, Marc Swidergall, Scott G Filler, Michael R Yeaman, Ashraf S Ibrahim
Different pathogens share similar medical settings and rely on similar virulence strategies to cause infections. We have previously applied 3-D computational modeling and bioinformatics to discover novel antigens that target more than one human pathogen. Active and passive immunization with the recombinant N-terminus of Candida albicans Hyr1 (rHyr1p-N) protect mice against lethal candidemia. Here we determine that Hyr1p shares homology with cell surface proteins of the multidrug resistant Gram negative bacterium, Acinetobacter baumannii including hemagglutinin (FhaB) and outer membrane protein A (OmpA)...
May 10, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29720131/intestinal-candida-parapsilosis-isolates-from-rett-syndrome-subjects-bear-potential-virulent-traits-and-capacity-to-persist-within-the-host
#4
Francesco Strati, Antonio Calabrò, Claudio Donati, Claudio De Felice, Joussef Hayek, Olivier Jousson, Silvia Leoncini, Daniela Renzi, Lisa Rizzetto, Carlotta De Filippo, Duccio Cavalieri
BACKGROUND: Rett syndrome (RTT) is a neurological disorder mainly caused by mutations in MeCP2 gene. It has been shown that MeCP2 impairments can lead to cytokine dysregulation due to MeCP2 regulatory role in T-helper and T-reg mediated responses, thus contributing to the pro-inflammatory status associated with RTT. Furthermore, RTT subjects suffer from an intestinal dysbiosis characterized by an abnormal expansion of the Candida population, a known factor responsible for the hyper-activation of pro-inflammatory immune responses...
May 2, 2018: BMC Gastroenterology
https://www.readbyqxmd.com/read/29689455/impact-of-modified-diamond-like-carbon-coatings-on-the-spatial-organization-and-disinfection-of-mixed-biofilms-composed-of-escherichia-coli-and-pantoea-agglomerans-industrial-isolates
#5
L C Gomes, J Deschamps, R Briandet, F J Mergulhão
This work investigated the effects of diamond-like carbon (DLC) coatings on the architecture and biocide reactivity of dual-species biofilms mimicking food processing contaminants. Biofilms were grown using industrial isolates of Escherichia coli and Pantoea agglomerans on bare stainless steel (SST) and on two DLC surface coatings (a-C:H:Si:O designated by SICON® and a-C:H:Si designated by SICAN) in order to evaluate their antifouling activities. Quantification and spatial organization in single- and dual-species biofilms were examined by confocal laser scanning microscopy (CLSM) using a strain specific labelling procedure...
April 12, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29671721/the-bceabrs-four-component-system-that-is-essential-for-cell-envelope-stress-response-is-involved-in-sensing-and-response-to-host-defence-peptides-and-is-required-for-the-biofilm-formation-and-fitness-of-streptococcus-mutans
#6
Xiao-Lin Tian, Hasan Salim, Gaofeng Dong, Madison Parcells, Yung-Hua Li
PURPOSE: Streptococcus mutans is a primary cariogenic pathogen worldwide. In dental biofilms, S. mutans often faces life-threatening insults, such as killing by antimicrobial compounds from competing species and from the host. How such insults affect the physiology and virulence of S. mutans is poorly understood. In this study, we explored this question by investigating the responses of S. mutans strains to several host defence peptides and bacitracin. METHODOLOGY: S...
April 19, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29668945/the-activity-of-bacterial-peptidylarginine-deiminase-is-important-during-formation-of-dual-species-biofilm-by-periodontal-pathogen-porphyromonas-gingivalis-and-opportunistic-fungus-candida-albicans
#7
Karkowska-Kuleta Justyna, Dominika Bartnicka, Marcin Zawrotniak, Gabriela Zielinska, Anna Kieronska, Oliwia Bochenska, Izabela Ciaston, Joanna Koziel, Jan Potempa, Zbigniew Baster, Zenon Rajfur, Maria Rapala-Kozik
Porphyromonas gingivalis, an anaerobic Gram-negative bacterium critically involved in the development of human periodontitis, belongs to the late colonizers of the oral cavity. The success of this pathogen in the host colonization and infection results from the presence of several virulence factors, including extracellular peptidylarginine deiminase (PPAD), an enzyme that converts protein arginine residues to citrullines. A common opportunistic fungal pathogen of humans, Candida albicans, is also frequently identified among microorganisms that reside at subgingival sites...
April 12, 2018: Pathogens and Disease
https://www.readbyqxmd.com/read/29665443/activity-of-a-nitric-oxide-generating-wound-treatment-system-against-wound-pathogen-biofilms
#8
Richard D Waite, Joanne E Stewart, Abish S Stephen, Robert P Allaker
Wound bioburden plays an important role in impaired healing and the development of infection-related complications. The objective of this study was to determine the efficacy of an innovative two-layer nitric oxide generating system (NOx) to prevent and treat biofilms formed by bacterial and fungal pathogens commonly associated with wound infection, and activity against Pseudomonas aeruginosa virulence factors. Single and mixed species biofilms were grown for 24h on nitrocellulose filters placed on agar. Filters were covered with either NOx or placebo, before and after biofilm formation...
April 14, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29663827/potential-of-antimicrobial-treatment-of-linear-low-density-polyethylene-with-poly-tert-butyl-amino-methyl-styrene-to-reduce-biofilm-formation-in-the-food-industry
#9
Carina Hüwe, Jennifer Schmeichel, Florian Brodkorb, Sophia Dohlen, Katrin Kalbfleisch, Martin Kreyenschmidt, Reinhard Lorenz, Judith Kreyenschmidt
Antimicrobial surfaces are one approach to prevent biofilms in the food industry. The aim of this study was to investigate the effect of poly((tert-butyl-amino)-methyl-styrene) (poly(TBAMS)) incorporated into linear low-density polyethylene (LLDPE) on the formation of mono- and mixed-species biofilms. The biofilm on untreated and treated LLDPE was determined after 48 and 168 h. The comparison of the results indicated that the ability of Listeria monocytogenes to form biofilms was completely suppressed by poly(TBAMS) (Δ168 h 3...
April 17, 2018: Biofouling
https://www.readbyqxmd.com/read/29562719/antibiofilm-effect-of-dnase-against-single-and-mixed-species-biofilm
#10
Komal Sharma, Ankita Pagedar Singh
Biofilms are aggregates of microorganisms that coexist in socially coordinated micro-niche in a self-produced polymeric matrix on pre-conditioned surfaces. The biofilm matrix reduces the efficacy of antibiofilm strategies. DNase degrades the extracellular DNA (e-DNA) present in the matrix, rendering the matrix weak and susceptible to antimicrobials. In the current study, the effect of DNase I was evaluated during biofilm formation (pre-treatment), on preformed biofilms (post-treatment) and both (dual treatment)...
March 19, 2018: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/29555180/non-tuberculous-mycobacteria-multispecies-biofilms-in-cystic-fibrosis-development-of-an-in-vitro-mycobacterium-abscessus-and-pseudomonas-aeruginosa-dual-species-biofilm-model
#11
Graciela Rodríguez-Sevilla, Marta García-Coca, David Romera-García, John Jairo Aguilera-Correa, Ignacio Mahíllo-Fernández, Jaime Esteban, Concepción Pérez-Jorge
Lung disease in cystic fibrosis (CF) is characterized by the progressive colonization of the respiratory tract by different bacteria, which develop polymicrobial biofilms. In the past decades, there has been an increase in the number of CF patients infected with Non-Tuberculous Mycobacteria (NTM). Although Mycobacterium abscessus is the main NTM isolated globally, little is known about M. abscessus multispecies biofilm formation. In the present study we developed an in vitro model to study the phenotypic characteristics of biofilms formed by M...
April 2018: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29520965/development-of-a-mixed-species-biofilm-model-and-its-virulence-implications-in-device-related-infections
#12
Naing T Thet, Laura Wallace, Anne Wibaux, Nick Boote, A Toby A Jenkins
It is becoming increasingly accepted that to understand and model the bacterial colonization and infection of abiotic surfaces requires the use of a biofilm model. There are many bacterial colonizations by at least two primary species, however this is difficult to model in vitro. This study reports the development of a simple mixed-species biofilm model using strains of two clinically significant bacteria: Staphylococcus aureus and Pseudomonas aeruginosa grown on nanoporous polycarbonate membranes on nutrient agar support...
March 8, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29458673/denture-associated-biofilm-infection-in-three-dimensional-oral-mucosal-tissue-models
#13
Daniel J Morse, Melanie J Wilson, Xiaoqing Wei, Michael A O Lewis, David J Bradshaw, Craig Murdoch, David W Williams
PURPOSE: In vitro analyses of virulence, pathogenicity and associated host cell responses are important components in the study of biofilm infections. The Candida-related infection, denture-associated oral candidosis, affects up to 60 % of denture wearers and manifests as inflammation of palatal tissues contacting the denture-fitting surface. Commercially available three-dimensional tissue models can be used to study infection, but their use is limited for many academic research institutions, primarily because of the substantial purchase costs...
March 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29457734/role-of-nutrient-limitation-in-the-competition-between-uropathogenic-strains-of-klebsiella-pneumoniae-and-escherichia-coli-in-mixed-biofilms
#14
Guillermo E Juarez, Estela M Galván
Klebsiella pneumoniae and Escherichia coli form mixed species biofilms in catheter-associated urinary tract infections. Recently, a detrimental effect of K. pneumoniae over E. coli was observed in mixed species biofilms grown in an artificial urine medium. The mechanism behind this competitive interaction was studied. K. pneumoniae partially outcompeted E. coli in early-stage batch-fed biofilms, whereas both microorganisms co-exist at longer times (K. pneumoniae:E. coli ratio, 55:1), as shown by cell counts and confocal microscopy...
February 19, 2018: Biofouling
https://www.readbyqxmd.com/read/29357385/infectious-blood-source-alters-early-foregut-infection-and-regurgitative-transmission-of-yersinia-pestis-by-rodent-fleas
#15
David M Bland, Clayton O Jarrett, Christopher F Bosio, B Joseph Hinnebusch
Fleas can transmit Yersinia pestis by two mechanisms, early-phase transmission (EPT) and biofilm-dependent transmission (BDT). Transmission efficiency varies among flea species and the results from different studies have not always been consistent. One complicating variable is the species of rodent blood used for the infectious blood meal. To gain insight into the mechanism of EPT and the effect that host blood has on it, fleas were fed bacteremic mouse, rat, guinea pig, or gerbil blood; and the location and characteristics of the infection in the digestive tract and transmissibility of Y...
January 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29288452/application-of-rna-seq-and-bioimaging-methods-to-study-microbe-microbe-interactions-and-their-effects-on-biofilm-formation-and-gene-expression
#16
Cristina Isabel Amador, Claus Sternberg, Lars Jelsbak
Complex interactions between pathogenic bacteria, the microbiota, and the host can modify pathogen physiology and behavior. We describe two different experimental approaches to study microbe-microbe interactions in in vitro systems containing surface-associated microbial populations. One method is the application of RNA sequencing (RNA-seq) to determine the transcriptional changes in pathogenic bacteria in response to microbial interspecies interactions. The other method combines flow cell devices for bacterial cultivation and growth with high-resolution bioimaging to analyze the microscale structural organization of interacting microbial populations within mixed-species biofilms...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29284195/extracellular-matrix-influence-in-streptococcus-mutans-gene-expression-in-a-cariogenic-biofilm
#17
E J Florez Salamanca, M I Klein
Caries etiology is biofilm-diet-dependent. Biofilms are highly dynamic and structured microbial communities enmeshed in a three-dimensional extracellular matrix. The study evaluated the expression dynamics of Streptococcus mutans genes associated with exopolysaccharides (EPS) (gtfBCD, gbpB, dexA), lipoteichoic acids (LTA) (dltABCD, SMU_775c) and extracellular DNA (eDNA) (lytST, lrgAB, ccpA) during matrix development within a mixed-species biofilm of S. mutans, Actinomyces naeslundii and Streptococcus gordonii...
April 2018: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/29275280/dynamics-of-biofilm-formation-by-listeria-monocytogenes-on-stainless-steel-under-mono-species-and-mixed-culture-simulated-fish-processing-conditions-and-chemical-disinfection-challenges
#18
Eleni Papaioannou, Efstathios D Giaouris, Panagiotis Berillis, Ioannis S Boziaris
The progressive ability of a six-strains L. monocytogenes cocktail to form biofilm on stainless steel (SS), under fish-processing simulated conditions, was investigated, together with the biocide tolerance of the developed sessile communities. To do this, the pathogenic bacteria were left to form biofilms on SS coupons incubated at 15°C, for up to 240h, in periodically renewable model fish juice substrate, prepared by aquatic extraction of sea bream flesh, under both mono-species and mixed-culture conditions...
February 21, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29228383/oral-fungal-bacterial-biofilm-models-in-vitro-a-review
#19
Marlene Chevalier, Stephane Ranque, Isabelle Prêcheur
Inclusion of fungi as commensals in oral biofilm is an important innovation in oral biology, and this work aimed to review the literature on the available biofilm and related disease in vitro models. Actually, thousands of bacterial and around one hundred of fungal phylotypes can colonize the oral cavity. Taxonomic profiling combined with functional expression analysis has revealed that Candida albicans, Streptococcus mutans and prominent periodontopathogens are not always present or numerically important in candidiasis, caries, or periodontitis lesions...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29211119/study-of-microbial-interaction-formed-by-candida-krusei-and-candida-glabrata-in-vitro-and-in-vivo-studies
#20
Rodnei Dennis Rossoni, Patrícia Pimentel de Barros, Fernanda Freire, Jéssica Diane Dos Santos, Antonio Olavo Cardoso Jorge, Juliana Campos Junqueira
Recently, the non-albicans Candida species have become recognized as an important source of infection and oral colonization by association of different species in a large number of immunosuppressed patients. The objective of this study was to evaluate the interactions between C. krusei and C. glabrata in biofilms formed in vitro and their ability to colonize the oral cavity of mouse model. Monospecies and mixed biofilms were developed of each strain, on 96-well microtiter plates for 48 h. These biofilms were analyzed by counting colony-forming units (CFU/mL) and by determining cell viability, using the XTT hydroxide colorimetric assay...
November 2017: Brazilian Dental Journal
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