keyword
MENU ▼
Read by QxMD icon Read
search

Mixed species biofilm

keyword
https://www.readbyqxmd.com/read/29288452/application-of-rna-seq-and-bioimaging-methods-to-study-microbe-microbe-interactions-and-their-effects-on-biofilm-formation-and-gene-expression
#1
Cristina Isabel Amador, Claus Sternberg, Lars Jelsbak
Complex interactions between pathogenic bacteria, the microbiota, and the host can modify pathogen physiology and behavior. We describe two different experimental approaches to study microbe-microbe interactions in in vitro systems containing surface-associated microbial populations. One method is the application of RNA sequencing (RNA-seq) to determine the transcriptional changes in pathogenic bacteria in response to microbial interspecies interactions. The other method combines flow cell devices for bacterial cultivation and growth with high-resolution bioimaging to analyze the microscale structural organization of interacting microbial populations within mixed-species biofilms...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29284195/extracellular-matrix-influence-in-streptococcus-mutans-gene-expression-in-a-cariogenic-biofilm
#2
Elkin Jahir Florez Salamanca, Marlise Inêz Klein
Caries etiology is biofilm-diet dependent. Biofilms are highly dynamic and structured microbial communities enmeshed in a three-dimensional extracellular matrix. The study evaluated the expression dynamics of Streptococcus mutans genes associated with exopolysaccharides (EPS) (gtfBCD, gbpB, dexA), lipoteichoic acids (LTA) (dltABCD, SMU_775c) and extracellular DNA (eDNA) (lytST, lrgAB, ccpA) during matrix development within a mixed-species biofilm of S. mutans, Actinomyces naeslundii and Streptococcus gordonii...
December 28, 2017: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/29275280/dynamics-of-biofilm-formation-by-listeria-monocytogenes-on-stainless-steel-under-mono-species-and-mixed-culture-simulated-fish-processing-conditions-and-chemical-disinfection-challenges
#3
Eleni Papaioannou, Efstathios D Giaouris, Panagiotis Berillis, Ioannis S Boziaris
The progressive ability of a six-strains L. monocytogenes cocktail to form biofilm on stainless steel (SS), under fish-processing simulated conditions, was investigated, together with the biocide tolerance of the developed sessile communities. To do this, the pathogenic bacteria were left to form biofilms on SS coupons incubated at 15°C, for up to 240h, in periodically renewable model fish juice substrate, prepared by aquatic extraction of sea bream flesh, under both mono-species and mixed-culture conditions...
December 19, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29228383/oral-fungal-bacterial-biofilm-models-in-vitro-a-review
#4
Marlene Chevalier, Stephane Ranque, Isabelle Prêcheur
Inclusion of fungi as commensals in oral biofilm is an important innovation in oral biology, and this work aimed to review the literature on the available biofilm and related disease in vitro models. Actually, thousands of bacterial and around one hundred of fungal phylotypes can colonize the oral cavity. Taxonomic profiling combined with functional expression analysis has revealed that Candida albicans, Streptococcus mutans and prominent periodontopathogens are not always present or numerically important in candidiasis, caries, or periodontitis lesions...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29211119/study-of-microbial-interaction-formed-by-candida-krusei-and-candida-glabrata-in-vitro-and-in-vivo-studies
#5
Rodnei Dennis Rossoni, Patrícia Pimentel de Barros, Fernanda Freire, Jéssica Diane Dos Santos, Antonio Olavo Cardoso Jorge, Juliana Campos Junqueira
Recently, the non-albicans Candida species have become recognized as an important source of infection and oral colonization by association of different species in a large number of immunosuppressed patients. The objective of this study was to evaluate the interactions between C. krusei and C. glabrata in biofilms formed in vitro and their ability to colonize the oral cavity of mouse model. Monospecies and mixed biofilms were developed of each strain, on 96-well microtiter plates for 48 h. These biofilms were analyzed by counting colony-forming units (CFU/mL) and by determining cell viability, using the XTT hydroxide colorimetric assay...
November 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/29208208/corrigendum-to-photoinactivation-of-single-and-mixed-biofilms-of-candida-albicans-and-non-albicans-candida-species-using-photodithazine%C3%A2-photodiagn-photodyn-ther-17c-2017-194-199
#6
Juliana Cabrini Carmello, Fernanda Alves, Ewerton Garcia de Oliveira Mima, Janaina Habib Jorge, Vanderlei Salvador Bagnato, Ana Cláudia Pavarina
No abstract text is available yet for this article.
December 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29184101/a-meta-proteomics-approach-to-study-the-interspecies-interactions-affecting-microbial-biofilm-development-in-a-model-community
#7
Jakob Herschend, Zacharias B V Damholt, Andrea M Marquard, Birte Svensson, Søren J Sørensen, Per Hägglund, Mette Burmølle
Microbial biofilms are omnipresent in nature and relevant to a broad spectrum of industries ranging from bioremediation and food production to biomedical applications. To date little is understood about how multi-species biofilm communities develop and function on a molecular level, due to the complexity of these biological systems. Here we apply a meta-proteomics approach to investigate the mechanisms influencing biofilm formation in a model consortium of four bacterial soil isolates; Stenotrophomonas rhizophila, Xanthomonas retroflexus, Microbacterium oxydans and Paenibacillus amylolyticus...
November 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29178712/role-of-tyrosol-on-candida-albicans-candida-glabrata-and-streptococcus-mutans-biofilms-developed-on-different-surfaces
#8
Douglas Roberto Monteiro, Laís Salomão Arias, Renan Aparecido Fernandes, Fabiana Gouveia Straioto, Débora Barros Barbosa, Juliano Pelim Pessan, Alberto Carlos Botazzo Delbem
PURPOSE: To assess the effect of tyrosol on the production of hydrolytic enzymes (by Candida biofilm cells) and acid (by Streptococcus mutans biofilms), as well as to quantify single and mixed biofilms of these species formed on acrylic resin (AR) and hydroxyapatite (HA). METHODS: Candida and S. mutans biofilms were formed on AR and HA in the presence of tyrosol during 48 hours. Next, acid proteinase, phospholipase and hemolytic activities of Candida biofilm cells were determined, while acid production by S...
February 2017: American Journal of Dentistry
https://www.readbyqxmd.com/read/29124888/differential-oxidative-stress-tolerance-of-streptococcus-mutans-isolates-affects-competition-in-an-ecological-mixed-species-biofilm-model
#9
Yuan Liu, Sara R Palmer, Hsiaochi Chang, Ashton N Combs, Robert A Burne, Hyun Koo
Streptococcus mutans strongly influences the development of pathogenic biofilms associated with dental caries. Our understanding of S. mutans behavior in biofilms is based on a few well-characterized laboratory strains; however, individual isolates vary widely in genome content and virulence-associated phenotypes, such as biofilm formation and environmental stress sensitivity. Using an ecological biofilm model, we assessed the impact of co-cultivation of several S. mutans isolates with Streptococcus oralis and Actinomyces naeslundii on biofilm composition following exposure to sucrose...
November 10, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/29123396/use-of-magnetic-nanoparticles-as-a-drug-delivery-system-to-improve-chlorhexidine-antimicrobial-activity
#10
Grażyna Tokajuk, Katarzyna Niemirowicz, Piotr Deptuła, Ewelina Piktel, Mateusz Cieśluk, Agnieszka Z Wilczewska, Jan R Dąbrowski, Robert Bucki
Nanotechnology offers new tools for developing therapies to prevent and treat oral infections, particularly biofilm-dependent disorders, such as dental plaques and endodontic and periodontal diseases. Chlorhexidine (CHX) is a well-characterized antiseptic agent used in dentistry with broad spectrum activity. However, its application is limited due to inactivation in body fluid and cytotoxicity toward human cells, particularly at high concentrations. To overcome these limitations, we synthesized nanosystems composed of aminosilane-coated magnetic nanoparticles functionalized with chlorhexidine (MNP@CHX)...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29111321/inhibition-activity-of-lactobacilli-supernatant-against-fungal-bacterial-multispecies-biofilms-on-silicone
#11
Yulong Tan, Matthias Leonhard, Doris Moser, Berit Schneider-Stickler
Fungal-bacterial multispecies biofilms play a major role in failure of medical silicone devices, such as voice prostheses in laryngectomiy. In this study, we determined the effect of Lactobacilli supernatant (cell free) on mixed biofilm formation of fungi and bacteria on silicone in vitro. Lactobacilli supernatant inhibited the adhesion (90 min) of mixed fungi and bacteria species with an efficiency of >90%. Mixed biofilm formation and the metabolic activity of the biofilms were inhibited by 72.23% and 58...
October 28, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29101060/dispersal-of-single-and-mixed-non-albicans-candida-species-biofilms-by-%C3%AE-1-3-glucanase-in-vitro
#12
Yulong Tan, Matthias Leonhard, Su Ma, Doris Moser, Berit Schneider-Stickler
β-1,3-glucan plays a role in non-albicans Candida species biofilm formation and survival of biofilm Candida to stresses. In this study, we evaluated the antibiofilm activity of β-1,3-glucanase, which can degrade poly-β(1 → 3)-glucose of non-albicans Candida species biofilms, on single and mixed species biofilm of non-albicans Candida species, including Candida tropicalis, Candida parapsilosis and Candida krusei. Biofilm by all tested species in microplate were dispersed more than 60%. β-1,3-glucanase also detached mixed species biofilm in microplate and on medical material surface...
November 1, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29099398/electric-field-based-dressing-disrupts-mixed-species-bacterial-biofilm-infection-and-restores-functional-wound-healing
#13
Kasturi Ganesh Barki, Amitava Das, Sriteja Dixith, Piya Das Ghatak, Shomita Mathew-Steiner, Elizabeth Schwab, Savita Khanna, Daniel J Wozniak, Sashwati Roy, Chandan K Sen
OBJECTIVE: This study was designed to employ electroceutical principles, as an alternative to pharmacological intervention, to manage wound biofilm infection. Mechanism of action of a United States Food and Drug Administration-cleared wireless electroceutical dressing (WED) was tested in an established porcine chronic wound polymicrobial biofilm infection model involving inoculation with Pseudomonas aeruginosa PAO1 and Acinetobacter baumannii 19606. BACKGROUND: Bacterial biofilms represent a major wound complication...
November 2, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/29079615/%C3%AE-glucanase-activity-of-the-oral-bacterium-tannerella-forsythia-contributes-to-the-growth-of-a-partner-species-fusobacterium-nucleatum-in-co-biofilms
#14
Kiyonobu Honma, Angela Ruscitto, Ashu Sharma
Tannerella forsythia and Fusobacterium nucleatum are dental plaque bacteria implicated in the development of periodontitis. These two species have been shown to form synergistic biofilms and found to be closely associated in dental plaque biofilms. A number of genetic loci for TonB-dependent membrane receptors (TDR) for glycan acquisition, with many existing in association with genes coding for enzymes involved in the breakdown of complex glycans, have been identified in T. forsythia In this study, we focused on a locus, BFO_0186 -- BFO_0188, that codes for a predicted TDR-SusD transporter along with a putative β-glucan hydrolyzing enzyme (BFO_0186)...
October 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29078172/conductive-properties-of-methanogenic-biofilms
#15
Cheng Li, Keaton Larson Lesnik, Hong Liu
Extracellular electron transfer between syntrophic partners needs to be efficiently maintained in methanogenic environments. Direct extracellular electron transfer via electrical current is an alternative to indirect hydrogen transfer but requires construction of conductive extracellular structures. Conductive mechanisms and relationship between conductivity and the community composition in mixed-species methanogenic biofilms are not well understood. The present study investigated conductive behaviors of methanogenic biofilms and examined the correlation between biofilm conductivity and community composition between different anaerobic biofilms enriched from the same inoculum...
October 19, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/29075322/pulse-electromagnetic-fields-enhance-extracellular-electron-transfer-in-magnetic-bioelectrochemical-systems
#16
Huihui Zhou, Bingfeng Liu, Qisong Wang, Jianmin Sun, Guojun Xie, Nanqi Ren, Zhiyong Jason Ren, Defeng Xing
BACKGROUND: Microbial extracellular electron transfer (EET) is essential in driving the microbial interspecies interaction and redox reactions in bioelectrochemical systems (BESs). Magnetite (Fe3O4) and magnetic fields (MFs) were recently reported to promote microbial EET, but the mechanisms of MFs stimulation of EET and current generation in BESs are not known. This study investigates the behavior of current generation and EET in a state-of-the-art pulse electromagnetic field (PEMF)-assisted magnetic BES (PEMF-MBES), which was equipped with magnetic carbon particle (Fe3O4@N-mC)-coated electrodes...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29031236/inhibitory-effect-of-probiotic-lactobacilli-supernatants-on-single-and-mixed-non-albicans-candida-species-biofilm
#17
Yulong Tan, Matthias Leonhard, Doris Moser, Su Ma, Berit Schneider-Stickler
OBJECTIVES: Oral candidiasis is one of the most common human fungal infections. While most cases of the Candida species isolated from the oral cavity are Candida albicans, a large number of candidiasis is attributed to non-albicans Candida species. In this study, we aim to evaluate the in vitro inhibition of supernatants of Lactobacillus gasseri and Lactobacillus rhamnosus on the single and mixed species biofilm of non-albicans Candida species, including Candida tropicalis, Candida krusei and Candida parapsilosis...
October 6, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29027368/expression-of-fluorescent-proteins-in-lactobacillus-rhamnosus-to-study-host-microbe-and-microbe-microbe-interactions
#18
Irina Spacova, Elke Lievens, Tine Verhoeven, Hans Steenackers, Jos Vanderleyden, Sarah Lebeer, Mariya I Petrova
Probiotic Lactobacillus strains are widely used to benefit human and animal health, although the exact mechanisms behind their interactions with the host and the microbiota are largely unknown. Fluorescent tagging of live probiotic cells is an important tool to unravel their modes of action. In this study, the implementation of different heterologously expressed fluorescent proteins for the labelling of the model probiotic strains Lactobacillus rhamnosusGG (gastrointestinal) and Lactobacillus rhamnosusGR-1 (vaginal) was explored...
October 13, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/29025667/adhesion-forces-of-biofilms-developed-in-vitro-from-clinical-strains-of-skin-wounds
#19
Elizabeth Alvarado-Gomez, Mario Perez-Diaz, Donato Valdez-Perez, Jaime Ruiz-Garcia, Martin Magaña-Aquino, Gabriel Martinez-Castañon, Fidel Martinez-Gutierrez
A biofilm is a very complex consortium formed by a mix of different microorganisms, which have become an important health problem, because its formation is a resistance mechanism used by bacteria against antibiotics or the immune system. In this work, we show differences between some physicochemical properties of biofilms in mono- and multi-species, formed by bacteria from clinical samples of infected chronic wounds. Of the most prevalent bacteria in wounds, two mono- and one multi-species biofilms were developed in vitro by Drip Flow Reactor: one biofilm was developed by S...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28987620/evaluation-of-culture-conditions-for-mixed-biofilm-formation-with-clinically-isolated-non-albicans-candida-species-and-staphylococcus-epidermidis-on-silicone
#20
Yulong Tan, Matthias Leonhard, Berit Schneider-Stickler
Silicone is frequently used in clinical and medical fields for medical devices. Mixed biofilms composed of Candida and bacterial species causes frequently failure of medical silicone devices, In this in vitro study, we analyzed mixed biofilm formation of clinically isolated non-albicans Candida species and Staphylococcus epidermidis, including Candida tropicalis, Candida krusei and Candida parapsilosis under the influence of different growth media (RPMI 1640, BHI and TSB) and several culture variables (incubation period, feeding conditions and FBS)...
October 5, 2017: Microbial Pathogenesis
keyword
keyword
29499
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"