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Hwa-Young Lee, Hyung-Ryong Kim, Han-Jung Chae
ROS and its associated signaling contribute to vascular aging-associated endothelial disturbance. Since the non-effective endothelial nitric oxide synthase (eNOS) coupling status is related to vascular aging-related phenotypes, eNOS coupled/uncoupled system signaling was studied in human umbilical vein endothelial cells (HUVEC). Nitric oxide (NO) and eNOS Ser1177 were significantly decreased, whereas O2(-) (superoxide anion radical) increased with passage number. In aging cells, NADPH oxidase 4 (Nox4), one of the main superoxide generating enzymes, and its associated protein disulfide isomerase (PDI) chaperone were highly activated, and the resultant ER redox imbalance leads to disturbance of protein folding capability, namely endoplasmic reticulum (ER) stress, ultimately inducing dissociation between HSP90 and IRE-1α or PERK, decreasing HSP90 stability and dissociating the binding of eNOS from the HSP90 and leading to eNOS uncoupling...
September 12, 2017: Free Radical Biology & Medicine
Maximiliano Cagel, Ezequiel Bernabeu, Lorena Gonzalez, Eduardo Lagomarsino, Marcela Zubillaga, Marcela A Moretton, Diego A Chiappetta
Doxorubicin (DOX) is used as a "first-line" antineoplastic drug in ovarian and metastatic breast cancer. However, serious side effects, such as cardiotoxicity have been reported after DOX intravenous administration. Hence, we investigated different micelle-former biomaterials, as Soluplus(®), Pluronic F127, Tetronic T1107 and d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) to develop a potential mixed micellar nanocarrier for DOX delivery. Since DOX hydrochloride is a poor candidate to be encapsulated inside the hydrophobic core of the mixed micelles, we assayed a hydrophobic complex between DOX and sodium deoxycholate (NaDC) as an excellent candidate to be encapsulated within polymeric micelles...
September 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Sarbashri Bank, Pradipta Jana, G V Girish, Asru Sinha, Smarajit Maiti
Organic "nitro" compounds such as nitroglycerine, isosorbate nitrate are useful in the control of chest pain in acute coronary syndrome. But the mechanism of it in pain regulation remains speculative. Here, increase of NO production was investigated by the possible regulation of constitutive nitric oxide synthase (cNOS) function from goat arterial endothelial cells. This protein was purified and sequence wise characterized as protein disulfide isomerase (PDI) in response to different nitro compounds. The NO generating protein was isolated from arterial endothelial cells and prepared to homogeneity...
September 11, 2017: Protein and Peptide Letters
Yan Choi Lam, Robert J Nielsen, William A Goddard, Aswini K Dash
Density functional theory (DFT, B3LYP-D3 with implicit solvation in toluene) was used to investigate the mechanisms of olefin hydrosilylation catalyzed by PDI(Fe) (bis(imino)pyridine iron) complexes, where PDI = 2,6-(ArN[double bond, length as m-dash]CMe)2(C5H3N) with Ar = 2,6-R2-C6H3. We find that the rate-determining step for hydrosilylation is hydride migration from Et3SiH onto the Fe-bound olefin to form (PDI)Fe(alkyl)(SiEt3). This differs from the mechanism for the Pt Karstedt catalyst in that there is no prior Si-H oxidative addition onto the Fe center...
September 13, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
Khaled AboulFotouh, Ayat A Allam, Mahmoud El-Badry, Ahmed M El-Sayed
Candesartan cilexetil is widely used in the management of hypertension and heart failure. The drug delivery encounters obstacles of poor aqueous solubility, efflux by intestinal P-glycoprotein and vulnerability to enzymatic degradation in small intestine. Self-nanoemulsifying drug delivery systems (SNEDDS) loaded with candesartan cilexetil were successfully developed to overcome such obstacles. Preliminary screening was carried out to select proper surfactant, co-surfactant and oil combination for successful SNEDDS formulation...
September 6, 2017: European Journal of Pharmaceutical Sciences
Tingting Liu, Xinyuan Hu, Yanfeng Wang, Liyang Meng, Yanan Zhou, Jixiang Zhang, Min Chen, Xiaomei Zhang
With the increase of antibiotic resistances in microorganisms, photodynamic inactivation (PDI) as a clinically proven antibacterial therapy is gaining increasing attention in recent years due to its high efficacy. Herein, we reported two covalent organic frameworks (COFs) materials, namely COFs-Trif-Benz and COF-SDU1, as effective type-II photosensitizers for photodynamic inactivation of bacteria. COFs-Trif-Benz and COF-SDU1 are synthesized through a facile solvothermal reaction between tri-(4-formacylphenoxy)-1,3,5-triazine (trif) and benzidine or p-phenylenediamine with high yield...
July 21, 2017: Journal of Photochemistry and Photobiology. B, Biology
Qinqin Shi, Eric S Andreansky, Seth R Marder, Simon B Blakey
Coronenediimide (CDI) derivatives have a planar structure, a reasonably high electron affinity, and a rigid and extended delocalized π-system. Therefore, this core and variants thereof may be promising building blocks for the synthesis of electron transport materials. Herein, we have synthesized thiazole-semicoronenediimides (TsCDIs) and -coronenediimides (TCDIs) by a two-step process from a perylenediimide (PDI) precursor. Conditions for C-H arylation and heteroarylation of the thiazole moiety of this core were developed and were successfully used for the synthesis of dimer, triad, and polymeric materials...
September 8, 2017: Journal of Organic Chemistry
Xiao-Gang Chen, Fu Hua, Shou-Guo Wang, Hong-Hui Tang
Albumin-conjugated multilayered nanoemulsion (albumin-MNE) of methyl prednisolone (MP) was developed to ensure the specificity of the drug at the spinal cord injury (SCI) site. MNE was prepared by emulsification followed by ionic deposition of oppositely charged polymer followed by albumin conjugation using N-hydroxysuccinimide. Prepared nanoemulsion was characterized for particle size, polydispersity index (PDI), zeta potential (Zp), pH, viscosity, and entrapment efficiency. It was further evaluated for shape and morphological analysis, in vitro release, cell viability, and in vivo efficacy against post SCI-like conditions in terms of behavioral assessment, histopathological evaluation, and immunoflorescence assay of the histological sections showing Bax-driven apoptosis...
September 5, 2017: AAPS PharmSciTech
Kathleen Lange, Christian Lautenschläger, Maria Wallert, Stefan Lorkowski, Andreas Stallmach, Alexander Schiller
We here describe a new approach to visualise nitric oxide (NO) in living macrophages by fluorescent NO-sensitive microspheres based on poly(lactic-co-glycolic acid) (PLGA). PLGA microspheres loaded with NO550 dye were prepared through a modified solvent-evaporation method. Microparticles were characterized by a mean hydrodynamic diameter of 3000 nm, zeta potential of -26.000 ± 0.351 mV and a PDI of 0.828 ± 0.298. Under abiotic conditions, NO release was triggered through UV radiation (254 nm) of 10 mM sodium nitroprusside dehydrate (SNP)...
2017: Beilstein Journal of Nanotechnology
Mingyong Zhou, Keiona Khen, Taoran Wang, Qiaobin Hu, Jingyi Xue, Yangchao Luo
Egg yolk low density lipoprotein (LDL)/polysaccharide nanogels are newly explored as oral delivery systems with promising encapsulation potentials. Nonetheless, the stability of nanogels against aggregation in gastrointestinal tract remains a challenge. Therefore, chemical crosslinking by 1-ethyl-3-(3-dimethylaminopropyl) and carbodiimide/N-hydroxysuccinimide (EDC/NHS) was adopted to improve the gastrointestinal stability of nanogels. Compared to original uncrosslinked nanogels, crosslinking did not change particle size, polydispersity index (PDI) and morphology, but it reduced surface charge of nanogels...
January 15, 2018: Food Chemistry
Eric R Kennehan, Christopher Grieco, Alyssa N Brigeman, Grayson S Doucette, Adam Rimshaw, Kayla Bisgaier, Noel C Giebink, John B Asbury
Ultrafast vibrational spectroscopy in the mid-infrared was used to directly probe the delocalization of excitons in two different perylenediimide (PDI) derivatives that are predicted to preclude the formation of excimers, which can act as trap sites for excited state energy in organic semiconductors. We identified vibrational modes within the conjugated C-C stretch modes of PDI molecules whose frequencies reported the interactions of molecules within delocalized excitonic states. The vibrational linewidths of these modes, which we call intermolecular coordinate coupled (ICC) modes, provided a direct probe of the extent of exciton delocalization among the PDI molecules, which was confirmed using X-ray diffraction and electro-absorption spectroscopy...
September 4, 2017: Physical Chemistry Chemical Physics: PCCP
Kantaporn Kheawfu, Surachai Pikulkaew, Wasana Chaisri, Siriporn Okonogi
Clove oil ethanolic solution (CL-EtOH) have always been used for fish anesthesia. However, ethanol causes major side effect of fish hypersensivity. In this study, clove oil loaded nanoemulsion (CLN) was developed in order to enhance water miscibility of clove oil without using ethanol in the preparations. The obtained CLN was characterized in terms of droplet size, size distribution expressed as polydispersity index (PDI), and zeta potential. The anesthetic effect of CLN in comparison with CL-EtOH on Oreochromis niloticus (Nile tilapia) was investigated...
2017: Drug Discoveries & Therapeutics
Hideki Hiraoka, Kengo Nakahara, Yuki Kaneko, Shiori Akiyama, Kosaku Okuda, Takao Iwawaki, Masatake Fujimura, Yoshito Kumagai, Nobumasa Takasugi, Takashi Uehara
Methylmercury (MeHg) results in cell death through endoplasmic reticulum (ER) stress. Previously, we reported that MeHg induces S-mercuration at cysteine 383 or 386 in protein disulfide isomerase (PDI), and this modification induces the loss of enzymatic activity. Because PDI is a key enzyme for the maturation of nascent protein harboring a disulfide bond, the disruption in PDI function by MeHg results in ER stress via the accumulation of misfolded proteins. However, the effects of MeHg on unfolded protein response (UPR) sensors and their signaling remain unclear...
2017: Biological & Pharmaceutical Bulletin
Matthew D Jones, Xujun Wu, Julian Chaudhuri, Matthew G Davidson, Marianne J Ellis
Here a zirconium amine tris(phenolate) is used as the initiator for the production of polylactide for biomedical applications, as a replacement for a tin initiator (usually tin octanoate). The ring opening polymerization (ROP) was carried out in the melt at 130°C. The zirconium-catalyzed PLA (PLA-Zr) required 30min, resulting in a polydispersity index (PDI) of 1.17, compared to 1h and PDI=1.77 for tin-catalyzed PLA (PLA-Sn). PLA-Zr and PLA-Sn supported osteosarcoma cell (MG63) culture to the same extent (cell number, morphology, extracellular matrix production and osteogenic function) until day 14 when the PLA-Zr showed increased cell number, overall extracellular matrix production and osteogenic function...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Fang Li, Qingguo Xing, Yingchao Han, Yue Li, Wei Wang, Thalagalage Shalika Harshani Perera, Honglian Dai
Biocompatible, biodegradable and stimuli-responsive nanomaterials can be used as drug carriers and to achieve controlled drug delivery, which is crucial for treating tumors and lowering drug side effects. Calcium phosphate (CaP) nanoparticles and poly(acrylic acid) (PAA) hydrogels can be used as biocompatible and pH-responsive drug carriers. In this study, based on the ultrasound effect, PAA/CaP hybrid nanogels (approximately 100nm, PDI<0.2) are obtained via the cross-linking of CaP nanoparticles and PAA molecules between the Ca(2+) ions and -COOH groups...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Yanan Ding, Miaomiao Chen, Kaili Wu, Mingxing Chen, Lifang Sun, Zhenxue Liu, Zhiqiang Shi, Qingyun Liu
N,N'-di-caboxy methyl perylene diimides (PDI), as one of the most promising functional materials in optional chemosensing, was first used to combine with Co3O4 nanoparticles through a facile two-step hydrothermal method and obtain the PDI functionalized Co3O4 nanocomposites (PDI-Co3O4 NCs). PDI-Co3O4 NCs were characterized by a series of technical analysis including transmission electron microscope (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR), respectively. The experimental results indicated that the as-prepared PDI-Co3O4 NCs possessed the higher peroxidase-like activity than that of Co3O4 nanoparticles without PDI, and could rapidly catalyze oxidation reaction of the chromogenic substrate TMB in the presence of H2O2 to a blue product (oxTMB) observed by the naked eye...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Varun Arora, Ankur Sood, Jyoti Shah, R K Kotnala, Tapan K Jain
In this study, we have synthesized magnetic nanocomposites of magnetite nanoparticles coated with 6-aminohexanoic acid and pectin (MAP). The size of the aqueous dispersion of the nanocomposites was 147nm with a Polydispersity index (PDI) of 0.32, and the nanocomposites were stable in NaCl up to a concentration of 0.45% (w/v) after which they aggregated. The dispersion of the nanocomposites was stable in Dulbecco's Modified Eagle's medium (DMEM) in the presence of 5 and 10% fetal bovine serum (FBS). Curcumin was used as a model drug to evaluate the potential of the nanocomposites for drug delivery applications...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Han-Chun Chien, Chao-Huei Chen, Teh-Ming Wang, Ya-Chi Hsu, Ming-Chih Lin
BACKGROUND: For infants with very low birth weights (VLBW), their neurodevelopmental outcomes are thought to be associated with extra-uterine growth retardation (EUGR). In this study, based on a single institute, we analyzed the association between different levels or severity of EUGR of VLBW infants and their neurodevelopmental outcomes later at a corrected age of 24 months. METHODS: This is a hospital-based retrospective cohort study. The severity of EUGR was classified into three categories according to the z-score of discharge weight: z < -2...
August 11, 2017: Pediatrics and Neonatology
Carla Silva, Agatha Carretero, Diana Soares da Costa, Rui L Reis, Ramon Novoa-Carballal, Iva Pashkuleva
Heparin sulfate proteoglycans (HSPGs) are responsible for the storage and stabilization of numerous growth factors in the extracellular matrix. In this complex native environment, the efficient binding of the growth factors is determined by multivalent, specific and reversible electrostatic interactions between the sulfate groups of HSPGs and the positively charged amino acids of the growth factor. Inspired by this naturally occurring stabilization process, we propose the use of diblock copolymers of heparin and polyethylene glycol (Hep-b-PEG) for protection and delivery of FGF-2...
August 29, 2017: Acta Biomaterialia
Yang Fan, Qingjie Wang, Guimei Lin, Yanbin Shi, Zili Gu, Tingting Ding
In this study, novel prodrug-modified cationic liposome nanocomplexes (Combo NCs) were reported for gemcitabine (GEM) and docetaxel (DTX) co-delivery. This nanoplatform exhibited multiple favorable characteristics, such as a 'green' fabrication with a one-step chemical reaction, appropriate size (∼ 200 nm) and distribution (PDI < 0.2), low zeta potential (-31.1 mv), high drug-loading efficiency (9.3% GEM plus 3.1% DTX, wt%) and pH and enzymatic dual-stimulus-responsive release properties. Immunofluorescence and cellular uptake studies showed that Combo NCs efficiently targeted overexpressed CD44 in MDA-MB-231 carcinoma...
August 28, 2017: Acta Biomaterialia
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