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https://www.readbyqxmd.com/read/28239568/expression-of-hepatitis-b-virus-surface-antigens-induces-defective-gonad-phenotypes-in-caenorhabditis-elegans
#1
Yi-Yin Chen, Li-Wei Lee, Wei-Ning Hong, Szecheng J Lo
AIM: To test whether a simple animal, Caenorhabditis elegans (C. elegans), can be used as an alternative model to study the interaction between hepatitis B virus antigens (HBsAg) and host factors. METHODS: Three plasmids that were able to express the large, middle and small forms of HBsAgs (LHBsAg, MHBsAg, and SHBsAg, respectively) driven by a ubiquitous promoter (fib-1) and three that were able to express SHBsAg driven by different tissue-specific promoters were constructed and microinjected into worms...
February 12, 2017: World Journal of Virology
https://www.readbyqxmd.com/read/28236405/formulation-of-dried-lignans-nanosuspension-with-high-redispersibility-to-enhance-stability-dissolution-and-oral-bioavailability
#2
Gang Shen, Ling Cheng, Li-Qiang Wang, Li-Hong Zhang, Bao-De Shen, Wei-Bo Liao, Juan-Juan Li, Juan Zheng, Rong Xu, Hai-Long Yuan
Herpetospermum caudigerum lignans (HTL), one of the potential drugs with anti-hepatitis B virus and hepatoprotective effects, has limited clinical applications because of poor aqueous solubility and low bioavailability. Both herpetrione (HPE) and herpetin (HPN) are the most abundant ingredients in HTL and exhibit weak acidity. The purpose of the present study was to produce dried preparations of HTL (composed of HPE and HPN) nanosuspensions (HTL-NS) with high redispersibility using lyophilization technology...
October 2016: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28228551/cysteine-residues-in-a-yeast-viral-a-b-toxin-crucially-control-host-cell-killing-via-ph-triggered-disulfide-rearrangements
#3
Yutaka Suzuki, Sara L Schwartz, Nina C Mueller, Manfred J Schmitt
K28 is a viral A/B protein toxin that intoxicates yeast and fungal cells by endocytosis and retrograde transport to the endoplasmic reticulum (ER). While toxin translocation into the cytosol occurs on the oxidized α/β heterodimer, the precise mechanism of how the toxin crosses the ER membrane is unknown. Here we identified pH-triggered toxin-intrinsic thiol rearrangements that crucially control toxin conformation and host cell killing. In the natural habitat and low pH environment of toxin-secreting killer yeasts, K28 is structurally stable and biologically active as disulfide-bonded heterodimer while it forms inactive disulfide-bonded oligomers at neutral pH that are caused by activation and thiol deprotonation of β-subunit cysteines...
February 22, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28224375/lanolin-based-organogel-of-salicylic-acid-evidences-of-better-dermatokinetic-profile-in-imiquimod-induced-keratolytic-therapy-in-balb-c-mice-model
#4
Gajanand Sharma, Neelam Devi, Kanika Thakur, Ashay Jain, O P Katare
The primary aim of the present study was to develop lanolin-based organogel with enhanced delivery potential and reduced skin irritation for the treatment of hyperkeratotic lesions and scaling. The drug was encapsulated in the lipidic bilayers of organogel. The values of particle size, polydispersity index (PDI), and zeta potential of the developed carrier system was found to be 257.5 nm, 0.272, and -24.9 mV, respectively. The system was pseudoplastic in nature with the yield value of 2.3078 Pa. The skin permeation studies exhibited superiority of the prepared lanolin-based organogel formulation over the conventional gel formulation (CGF)...
February 21, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28224252/heat-shock-protein-70-in-lung-and-kidney-of-specific-pathogen-free-chickens-is-a-receptor-associated-protein-that-interacts-with-the-binding-domain-of-the-spike-protein-of-infectious-bronchitis-virus
#5
ZhiKun Zhang, Xin Yang, PengWei Xu, Xuan Wu, Long Zhou, HongNing Wang
Avian infectious bronchitis virus (IBV) is a member of the family Coronaviridae. A binding domain that mediates the attachment of the virus to its receptor has been identified in the S1 protein of prototype IBV strain M41. In this study, we identified this binding domain in a different strain, as well as the cellular proteins that interact with it. First, we expressed the S1N proteins (residues 19-270) of M41 and another isolate, SCZJ3, and compared the binding capacities of recombinant S1N-M41 and S1N-SCZJ3 to host tissues...
February 21, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28221716/ionic-self-assembled-platform-of-perylenediimide-sodium-dodecylsulfate-for-detection-of-spermine-in-clinical-samples
#6
Prabhpreet Singh, Lalit Singh Mittal, Gaurav Bhargava, Subodh Kumar
The detection and quantification of spermine in clinical practice is important for early diagnosis of many diseases. The chromatographic and immunoassay-based methods are commonly used. However, fluorescence-based assay could provide real time detection. Herein, we report synthesis and aggregation properties of dicationic perylene probe (DAB-PDI) and used DAB-PDI in developing fluorescent 'turn-on' ensemble for detection of spermine. The fluorescence of DAB-PDI (10 µM, Φ = 0.55) is efficiently quenched by negatively charged sodium dodecylsulfate (SDS) through formation of ionic self-assembled aggregates (charge ratio N/P = 9)...
February 21, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28218242/protein-disulfide-isomerase-secretion-following-vascular-injury-initiates-a-regulatory-pathway-for-thrombus-formation
#7
Sheryl R Bowley, Chao Fang, Glenn Merrill-Skoloff, Barbara C Furie, Bruce Furie
Protein disulfide isomerase (PDI), secreted by platelets and endothelial cells on vascular injury, is required for thrombus formation. Using PDI variants that form mixed disulfide complexes with their substrates, we identify by kinetic trapping multiple substrate proteins, including vitronectin. Plasma vitronectin does not bind to αvβ3 or αIIbβ3 integrins on endothelial cells and platelets. The released PDI reduces disulfide bonds on plasma vitronectin, enabling vitronectin to bind to αVβ3 and αIIbβ3...
February 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/28215702/nitroarachidonic-acid-no2-aa-inhibits-protein-disulfide-isomerase-pdi-through-reversible-covalent-adduct-formation-with-critical-cysteines
#8
Lucía González-Perilli, Mauricio Mastrogiovanni, Denise de Castro Fernandes, Homero Rubbo, Francisco Laurindo, Andrés Trostchansky
BACKGROUND: Nitroarachidonic acid (NO2AA) exhibits pleiotropic anti-inflammatory actions in a variety of cell types. We have recently shown that NO2AA inhibits phagocytic NADPH oxidase 2 (NOX2) by preventing the formation of the active complex. Recent work indicates the participation of protein disulfide isomerase (PDI) activity in NOX2 activation. Cysteine (Cys) residues at PDI active sites could be targets for NO2AA- nitroalkylation regulating PDI activity which could explain our previous observation...
February 12, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28213179/the-dispersion-releaser-technology-is-an-effective-method-for-testing-drug-release-from-nanosized-drug-carriers
#9
Christine Janas, Marc-Philip Mast, Li Kirsamer, Carlo Angioni, Fiona Gao, Werner Mäntele, Jennifer Dressman, Matthias G Wacker
The dispersion releaser (DR) is a dialysis-based setup for the analysis of the drug release from nanosized drug carriers. It is mounted into dissolution apparatus2 of the United States Pharmacopoeia. The present study evaluated the DR technique investigating the drug release of the model compound flurbiprofen from drug solution and from nanoformulations composed of the drug and the polymer materials poly (lactic acid), poly (lactic-co-glycolic acid) or Eudragit(®)RSPO. The drug loaded nanocarriers ranged in size between 185...
February 14, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28205647/microcin-pdi-regulation-and-proteolytic-cleavage-are-unique-among-known-microcins
#10
Zhe Zhao, Lisa H Orfe, Jinxin Liu, Shao-Yeh Lu, Thomas E Besser, Douglas R Call
Microcin PDI inhibits a diversity of pathogenic Escherichia coli through the action of an effector protein, McpM. In this study we demonstrated that expression of the inhibitory phenotype is induced under low osmolarity conditions and expression is primarily controlled by the EnvZ/OmpR two-component regulatory system. Functional, mutagenesis and complementation experiments were used to empirically demonstrate that EnvZ is required for the inhibitory phenotype and that regulation of mcpM is dependent on binding of the phosphorylated OmpR to the mcpM promoter region...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28202110/-neurodevelopmental-features-of-preterm-infants-at-the-corrected-age-of-1-year
#11
Mei Zhang, Hong-Yan Qian, Xiao-Ni Kuang, Zhong Yu, Lin Tan, Chan Xia
OBJECTIVE: To investigate the neurodevelopmental level of preterm infants at the corrected age of 1 year and the effect of complications on neurodevelopment. METHODS: The clinical data and follow-up data of hospitalized preterm infants were retrospectively studied. The Bayley Scales of Infant Development was used to assess the neurodevelopmental level. Preterm infants were divided into groups according to gestational age, birth weight, and the presence or absence of complications...
February 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/28198441/pdi-regulates-seizure-activity-via-nmda-receptor-redox-in-rats
#12
Ji Yang Kim, Ah-Rhem Ko, Hye-Won Hyun, Su-Ji Min, Ji-Eun Kim
Redox modulation of cysteine residues is one of the post-translational modifications of N-methyl-D-aspartate receptor (NMDAR). Protein disulfide isomerases (PDI), an endoplasmic reticulum (ER) chaperone, plays a crucial role in catalyzing disulfide bond formation, reduction, and isomerization. In the present study, we found that PDI bound to NMDAR in the normal hippocampus, and that this binding was increased in chronic epileptic rats. In vitro thiol reductase assay revealed that PDI increased the amount of thiols on full-length recombinant NR1 protein...
February 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28195742/enhanced-davydov-splitting-in-crystals-of-a-perylene-diimide-derivative
#13
Ashli Austin, Nicholas J Hestand, Ian G McKendry, Chuwei Zhong, Xuanyu Zhu, Michael J Zdilla, Frank C Spano, Jodi M Szarko
We report the polarized absorption spectra of high quality, thin crystals of a perylene diimide (PDI) species with branched side chains (B2). The absorption spectrum shows exemplary polarization-dependent H-like and J-like aggregate behavior upon orthogonal excitation, with a sizable Davydov splitting (DS) of 1230 cm-1 and peak to peak splitting of 3040 cm-1. The experimental results are compared to theoretical calculations with remarkable agreement. The theoretical analysis of the polarized absorption spectra shows evidence of a high degree of intermolecular charge transfer, which, along with Coulombic coupling, conspire to create the unprecedented DS for this family of dye molecules...
February 14, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28194872/preparation-and-fluorescence-properties-of-perylenediimide-nanodispersions-having-a-one-dimensional-%C3%AF-staking-structure
#14
Shino Sasaki, Tsuyoshi Asahi, Shohei Higuchi
We prepared stable nanodispersions of a fluorescent perylenbisimide (PDI) derivative having long alkyl chains by nanosecond laser fragmentation of its microcrystalline powder in acetonitrile (ACN). The nanoparticles had cubic-like or rod shapes with a mean size of 100 nm, and they dispersed stably for longer than 1 month. The prepared nanobricks exhibited the absorption and fluorescence spectra characteristic to one-dimensional aggregates of co-facial stacking of PDI planes. Single particle fluorescence measurements demonstrated that nanobricks had a well-aligned structure of one-dimensional column of PDI...
February 14, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28192006/does-marital-conflict-predict-infants-physiological-regulation-a-short-term-prospective-study
#15
Christin L Porter, W Justin Dyer
Prior research has linked marital conflict to children's internalizing/externalizing disorders, insecure attachment, and poor emotional regulation (e.g., Cummings & Davies, 2010; Cummings, Iannotti, & Zahn-Waxler, 1985). Although investigators have examined the impact of marital discord on older children (e.g., Crockenberg & Langrock, 2001), few have explored direct links in infancy (e.g., Cowan & Cowan, 1999). This study extends earlier work by examining linkages between marital functioning (conflict and harmony) and infants' cardiac vagal tone and developmental status across 2 time points using a cross-lag approach...
February 13, 2017: Journal of Family Psychology: JFP
https://www.readbyqxmd.com/read/28191974/single-molecule-fluorescence-spectroscopy-of-perylene-diimide-dyes-in-a-%C3%AE-cyclodextrin-film-manifestation-of-photoinduced-h-atom-transfer-via-higher-triplet-n-%C3%AF-excited-states
#16
Masaaki Mitsui, Hiroki Fukui, Ryoya Takahashi, Yasushi Takakura, Toshinari Mizukami
Supramolecular complexation of γ-cyclodextrin (γ-CD) with N,N'-bis(2,6-dimethylphenyl)perylene-3,4,9,10-tetracarboxylic diimide (DMP-PDI) or N,N'-bis(2,6-dioctyl)perylene-3,4,9,10-tetracarboxylic diimide (C8-PDI) dye in an aqueous solution and in a γ-CD solid film were investigated via ensemble and single-molecule fluorescence spectroscopy. These two perylene diimide derivatives possess almost the same electronic structure but have different terminal functional groups. This structural difference leads to formation of an inclusion complex of γ-CD with DMP-PDI but not with C8-PDI in aqueous solution...
February 13, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28187297/a-universal-aptameric-biosensor-multiplexed-detection-of-small-analytes-via-aggregated-perylene-based-broad-spectrum-quencher
#17
Rong Hu, Xi Zhang, Qiang Xu, Dan-Qing Lu, Yun-Hui Yang, Quan-Qing Xu, Qiong Ruan, Liu-Ting Mo, Xiao-Bing Zhang
A universal aptameric system based on the taking advantage of double-stranded DNA/perylene diimide (dsDNA/PDI) as the signal probe was developed for multiplexed detection of small molecules. Aptamers are single-stranded DNA or RNA oligonucleotides which are selected in vitro by a process known as systematic evolution of ligands by exponential enrichment. In this work, we synthesized a new kind of PDI and reported this aggregated PDI could quench the double-stranded DNA (dsDNA)-labeled fluorophores with a high quenching efficiency...
January 24, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28186986/common-cytotoxic-chemotherapeutics-induce-epithelial-mesenchymal-transition-emt-downstream-of-er-stress
#18
Parag P Shah, Tess V Dupre, Leah J Siskind, Levi J Beverly
Endoplasmic reticulum (ER) in eukaryotes is a main organelle involved in a wide variety of functions including calcium storage, lipid biosynthesis, protein folding and protein transport. Disruption of ER homeostasis leads to ER stress and activation of the unfolded protein response (UPR). We and others have previously found that ER stress induces EMT in different cellular systems. Induction of ER stress with chemical modulators of ER homeostasis was sufficient to activate an EMT-like state in all cellular systems tested...
February 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28186644/population-pharmacokinetics-and-pharmacodynamics-of-linezolid-induced-thrombocytopenia-in-hospitalized-patients
#19
Yasuhiro Tsuji, Nicholas H G Holford, Hidefumi Kasai, Chika Ogami, Young-A Heo, Yoshitsugu Higashi, Akiko Mizoguchi, Hideto To, Yoshihiro Yamamoto
AIMS: Thrombocytopenia is among the most important adverse effects of linezolid treatment. Linezolid-induced thrombocytopenia incidence varies considerably but has been associated with impaired renal function. We investigated the pharmacodynamic mechanism (myelosuppression or enhanced platelet destruction) and the role of impaired renal function in the development of thrombocytopenia. METHODS: The pharmacokinetics of linezolid were described with a two-compartment distribution model with first-order absorption and elimination...
February 10, 2017: British Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/28182110/a-preliminary-study-of-patient-dignity-inventory-validation-among-patients-hospitalized-in-an-acute-psychiatric-ward
#20
Rosaria Di Lorenzo, Giulio Cabri, Eleonora Carretti, Giacomo Galli, Nina Giambalvo, Giulia Rioli, Serena Saraceni, Giulia Spiga, Cinzia Del Giovane, Paola Ferri
PURPOSE: To investigate the perception of dignity among patients hospitalized in a psychiatric setting using the Patient Dignity Inventory (PDI), which had been first validated in oncologic field among terminally ill patients. PATIENTS AND METHODS: After having modified two items, we administered the Italian version of PDI to all patients hospitalized in a public psychiatric ward (Service of Psychiatric Diagnosis and Treatment of a northern Italian town), who provided their consent and completed it at discharge, from October 21, 2015 to May 31, 2016...
2017: Neuropsychiatric Disease and Treatment
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