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Fei Wang, Jing Wu, Sheng Chen
The recombinant plasmid pPIC9K-bgl1 containing β-glucosidase bgl1 from Trichoderma viride was constructed by overlapping PCR and integrated into Pichia pastoris KM71. In order to assist the formation of disulfide bonds and thus improve protein folding efficiency, protein disulfide isomerase pdi was co-expressed in the P. pastoris KM71/pPIC9K-bgl1/pPICZ-A-pdi strain, and fermentation in flasks resulted in enzyme activity of 143 U/ml. The enzyme activity of β-glucosidase reached 1402 U/ml following optimisation of fermentation conditions in a 3...
May 15, 2018: Food Chemistry
Rossella Dorati, Antonella DeTrizio, Melissa Spalla, Roberta Migliavacca, Laura Pagani, Silvia Pisani, Enrica Chiesa, Bice Conti, Tiziana Modena, Ida Genta
Nanotechnology is a promising approach both for restoring or enhancing activity of old and conventional antimicrobial agents and for treating intracellular infections by providing intracellular targeting and sustained release of drug inside infected cells. The present paper introduces a formulation study of gentamicin loaded biodegradable nanoparticles (Nps). Solid-oil-in water technique was studied for gentamicin sulfate nanoencapsulation using uncapped Polylactide-co-glycolide (PLGA-H) and Polylactide-co-glycolide-co-Polyethylenglycol (PLGA-PEG) blends...
January 12, 2018: Nanomaterials
K Dirian, S Bauroth, A Roth, Z Syrgiannis, F Rigodanza, M Burian, H Amenitsch, D I Sharapa, M Prato, T Clark, D M Guldi
The aggregation and the photophysics of a water soluble perylenediimide (PDI) derivative that features two bromine substituents in the bay positions has been probed. Non-fluorescent aggregates were found to be present at concentrations of 1.0 × 10-5 M. In situ small-angle X-ray scattering (SAXS) measurements and complementary molecular modeling showed the presence of PDI aggregates. In their singlet excited states, the PDI aggregates are characterized by distinct transient fingerprints and rapid deactivation, as revealed by pump-probe experiments on the femto-, pico-, nano-, and microsecond timescales...
January 12, 2018: Nanoscale
Rosa E Mares, Marco A Ramos
In eukaryotic cells, protein disulfide isomerases (PDI) are oxidoreductases that catalyze the proper disulfide bond formation during protein folding. The pathobiology of the protozoan parasite Entamoeba histolytica, the causative agent of human amebiasis, depends on secretion of several virulence factors, such as pore-forming peptides and cysteine proteinases. Although the native conformation of these factors is stabilized by disulfide bonds, there is little information regarding the molecular machinery involved in the oxidative folding of amebic proteins...
January 2018: FEBS Open Bio
Jiacheng Zhao, Mingxia Lu, Haiwang Lai, Hongxu Lu, Jacques Lalevée, Christopher Barner-Kowollik, Martina H Stenzel, Pu Xiao
Introducing a strategy towards polymer/nanodiamond hybrids with high polymer grafting density and accessible polymer structural characterisation is of critical importance for nanodiamonds surface modification and bioagent attachment for their biomedical application. Here, we report a glycopolymer/nanodiamond hybrid drug delivery system, which was prepared by grafting amonafide-conjugated glycopolymers onto the surface of nanodiamonds via oxime ligation. Poly(1-O-methacryloyl-2,3:4,5-di-O-isopropylidene-β-D-fructopyranose)-b-poly(3-vinylbenzaldehyde-co-methyl methacrylate), featuring pendant aldehyde groups, is prepared via RAFT polymerization...
January 9, 2018: Biomacromolecules
Mengyuan Cao, Xu Xue, Xixi Pei, Yiwen Qian, Lan Liu, Lili Ren, Guoguang Chen
The present research indicated that a new self-microemulsifying drug delivery systems (SMEDDS) were used to reduce the food effect of poorly water-soluble drug cinacalcet and enhance the bioavailability in beagle dogs by oral gavage. Ethyl oleate, OP-10 and PEG-200 was selected as the oil phase, surfactant and co-surfactant of cinacalcet-SMEDDS by the solubility and phase diagram studies. Central Composite Design-Response Surface Methodology was used to determine the ratio of surfactant and co-surfactant, the amount of oil for optimizing the SMEDDS formation...
January 9, 2018: Drug Development and Industrial Pharmacy
Hyun-Kyoung Kim, Raj Kumar Yadav, Kashi Raj Bhattarai, Han-Wool Jung, Hyung-Ryong Kim, Han-Jung Chae
STUDY QUESTION: Is the Transmembrane BAX Inhibitor Motif-6 (TMBIM6) involved in the molecular mechanism by which cisplatin causes reproductive toxicity? SUMMARY ANSWER: TMBIM6 protects against cisplatin-induced testicular toxicity through up-regulation of heme oxygenase-1 (HO-1),-which maintains the levels of steroidogenic enzymes by decreaseing oxidative stress in the endoplasmic reticulum (ER). WHAT IS KNOWN ALREADY: Testosterone production is highly suppressed as a main complication of cisplatin (cis-diamminedichloroplatinum) anticancer therapy...
January 4, 2018: Human Reproduction
E Oppersma, J Doorduin, J G van der Hoeven, P H Veltink, H W H van Hees, L M A Heunks
BACKGROUND: Patients with acute respiratory failure may develop respiratory acidosis. Metabolic compensation by bicarbonate production or retention results in posthypercapnic alkalosis with an increased arterial bicarbonate concentration. The hypothesis of this study was that elevated plasma bicarbonate levels decrease respiratory drive and minute ventilation. METHODS: In an intervention study in 10 healthy subjects the ventilatory response using a hypercapnic ventilatory response (HCVR) test was assessed, before and after administration of high dose sodium bicarbonate...
January 4, 2018: Respiratory Physiology & Neurobiology
Vesselin Kussovski, Vanya Mantareva, Mahmut Durmuş, Ivan Angelov
Photodynamic inactivation (PDI) has been featured as an effective strategy in the treatment of acute drug-resistant infections. The efficiency of PDI was evaluated against three periodontal pathogenic bacteria that were tested as drug-resistant strains. In vitro studies were performed with four water-soluble cationic Zn(II) phthalocyanines (ZnPc1-4) and irradiation of a specific light source (light-emitting diode, 665 nm) with three doses (15, 36 and 60 J/cm2). The well detectable fluorescence of ZnPcs allowed the cellular imaging, which suggested relatively high uptakes of ZnPcs into bacterial species...
January 8, 2018: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
Manal A Elsheikh, Yosra S R Elnaggar, Dina Y Otify, Ossama Y Abdallah
PURPOSE: To develop novel bioactive-chylomicrons to solve oral delivery obstacles of Berberine chloride and target the lymphatic system. METHODS: Berberine-loaded bioactive-chylomicrons were prepared and underwent full in vitro characterization. Intestinal permeability was appraised via both non-everted gut sac model and Caco-2 cell model. Furthermore, Bioactive-chylomicrons' cellular uptake and distribution were examined by laser scanning confocal microscopy. Finally, a novel chylomicron flow-blockage assay on tissue and cellular levels were elaborated to assess the lymphatic targeting ability...
January 5, 2018: Pharmaceutical Research
Michael C Yi, Arek V Melkonian, James A Ousey, Chaitan Khosla
Transglutaminase 2 (TG2) is a ubiquitously expressed, intracellular as well as extracellular protein with multiple modes of posttranslational regulation, including an allosteric disulfide bond between Cys370-Cys371 that renders the enzyme inactive in the extracellular matrix. Although recent studies have established that extracellular TG2 is switched "on" by the redox cofactor protein thioredoxin-1 (TRX), it is unclear how TG2 is switched "off". Here, we demonstrate that TG2 oxidation by biological small-molecule biological oxidants, including glutathione, cystine, and hydrogen peroxide, is unlikely to be the inactivation mechanism...
January 5, 2018: Journal of Biological Chemistry
Ruwini Dinushika Rajapaksha, Matthias Banet, Cody Champion, Liliya V Frolova, Snezna Rogelj, Pabitra Choudhury, Mahinda Iroshana Ranasinghe
Optical properties and fluorescence decay dynamics of a photoactive indole based antibacterial chromophore system, 2, 3-distyrylindole (23DSI), was investigated using various spectroscopic characterization techniques. These studies were done by utilizing the steady state UV-Vis spectroscopy, the steady state fluorescence spectroscopy, the time-resolve fluorescence upconversion spectroscopy, and time correlated single photon counting spectroscopy as experiment techniques. Our studies show that the 23DSI molecule has a multiphoton absorbing property as indicated by two and three photon absorption in both solution and solid phases...
January 5, 2018: Journal of Physical Chemistry. A
Julie Boisclair Demarble, Christophe Fortin, Bianca D'Antono, Stéphane Guay
Peritraumatic dissociation and distress are strong predictors of acute stress disorder (ASD) and posttraumatic stress disorder (PTSD) development. However, there is limited data concerning gender differences in these relations, particularly among victims of violent crimes (VVC). The objective of this study is to examine whether peritraumatic dissociation and distress predict the number of ASD symptoms differently for men and women VVC. In all, 162 adults (97 women, M age = 39.6 years), 63% of whom experienced physical assaults, completed the Acute Stress Disorder Interview, the Peritraumatic Dissociative Experience Questionnaire, and the Peritraumatic Distress Inventory...
February 1, 2017: Journal of Interpersonal Violence
Miguel O Jara, Johanna Catalan-Figueroa, Mariana Landin, Javier O Morales
Nanoprecipitation is a simple and fast method to produce polymeric nanoparticles (Np); however, most applications require filtration or another separation technique to isolate the nanosuspension from aggregates or polydisperse particle production. In order to avoid variability introduced by these additional steps, we report here a systematic study of the process to yield monomodal and uniform Np production with the nanoprecipitation method. To further identify key variables and their interactions, we used artificial neural networks (ANN) to investigate the multiple variables which influence the process...
December 29, 2017: Drug Delivery and Translational Research
Kai Zhang, Lei Lv, Xiaofen Wang, Yang Mi, Ruiqing Chai, Xinfeng Liu, Guozhen Shen, Aidong Peng, Hui Huang
Organic photodetectors (OPDs) have attracted great attention due to their advantages including tunable response range, easy processability and flexibility. Various conjugated polymers have been developed for high performing OPDs. Herein, a series of tellurophene-based random copolymers containing two typical electron-withdrawing units naphthalene diimide (NDI) and perylene diimide (PDI) are designed and synthesized. Through varying the ratio of PDI/NDI moieties of the analogous polymers, the opto-physical properties and film morphology, together with photodetector performances are systematically tuned...
December 28, 2017: ACS Applied Materials & Interfaces
Ruigang Lu, Yuxin Meng, Yan Zhang, Wei Zhao, Xun Wang, Mulan Jin, Ruijun Guo
BACKGROUND: Superb microvascular imaging (SMI) for depiction of microvascular flow in thyroid nodules was compared with color/power Doppler imaging (CDI/PDI) and contrast-enhanced ultrasonography (CEUS). In addition, the diagnostic performance of conventional ultrasound combined with SMI for differentiating benign and malignant thyroid nodules was evaluated. METHODS: Preoperative conventional ultrasound consisting of gray-scale ultrasonography and CDI/PDI, followed by SMI and CEUS, was used to record 52 thyroid nodules...
December 28, 2017: BMC Medical Imaging
Meghann C Ryan, Paul Sherman, Laura M Rowland, S Andrea Wijtenburg, Ashley Acheson, Els Fieremans, Jelle Veraart, Dmitry Novikov, L Elliot Hong, John Sladky, P Dana Peralta, Peter Kochunov, Stephen A McGuire
BACKGROUND: Neuroscience research in brain development and disorders can benefit from an in vivo animal model that portrays normal white matter (WM) development trajectories and has a sufficiently large cerebrum for imaging with human MRI scanners and protocols. NEW METHOD: Twelve three-month-old Sinclair™ miniature pigs (Sus scrofa domestica) were longitudinally evaluated during adolescent development using advanced diffusion weighted imaging (DWI) focused on cerebral WM...
December 22, 2017: Journal of Neuroscience Methods
Tobias Bornhütter, Nedaa Shamali, Irena Saltsman, Atif Mahammed, Zeev Gross, Georg Däschlein, Beate Röder
A treatment of onychomycosis using the photodynamic effect would be a favorable alternative to currently used antimycotic drugs. This study should be considered as a first step towards development and control of an efficient photodynamic inactivation of onychomycosis causative pathogens. Here, we evaluate the usage of time-resolved 2D singlet oxygen luminescence detection in combination with 2D fluorescence scanning as a tool to understand the behavior of the photosensitizer when applied to fungi on Petri dishes...
December 14, 2017: Journal of Photochemistry and Photobiology. B, Biology
Ahmed R Fares, Aliaa N ElMeshad, Mohamed A A Kassem
This study aims at preparing and optimizing lacidipine (LCDP) polymeric micelles using thin film hydration technique in order to overcome LCDP solubility-limited oral bioavailability. A two-factor three-level central composite face-centered design (CCFD) was employed to optimize the formulation variables to obtain LCDP polymeric micelles of high entrapment efficiency and small and uniform particle size (PS). Formulation variables were: Pluronic to drug ratio (A) and Pluronic P123 percentage (B). LCDP polymeric micelles were assessed for entrapment efficiency (EE%), PS and polydispersity index (PDI)...
November 2018: Drug Delivery
Zhitian Liu, Linhua Zhang, Ming Shao, Yao Wu, Di Zeng, Xiang Cai, Jiashun Duan, Xiaolu Zhang, Xiang Gao
The geometries of perylene diimides(PDI) based acceptors is important for improving the phase separation and charge transport in organic solar cells. In order to finely tune the geometry, diphenyl, spiro-fluorene and benzene were used as the core moiety to construct quasi-3D nonfullerene acceptors based on PDI building blocks. The molecular geometries, energy levels, optical properties, photovoltaic properties and exciton kinetics were systematically studied. The structure-performance relationship was discussed as well...
December 18, 2017: ACS Applied Materials & Interfaces
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