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https://www.readbyqxmd.com/read/28535501/is-the-morning-sputum-sample-superior-to-the-fresh-sputum-sample-for-the-detection-of-malignant-cells
#1
Maya S Uke, Saleem Pathuthara, Abida Shaikh, Rajiv Kumar, Shubhada Kane
INTRODUCTION: Sputum cytology is a well-established technique for the detection of lung malignancies. Generally, random or morning samples are used. OBJECTIVES: To evaluate the diagnostic yield of morning sputum and compare it with that of fresh samples. MATERIALS AND METHODS: Patients were instructed to bring a morning sputum sample to the laboratory in a clean plastic container, without any fixative and within 2-3 h of collection. Fresh sputum was then collected in the laboratory from these same patients...
May 24, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/28535206/clinical-exome-sequencing-reports-current-informatics-practice-and-future-opportunities
#2
Rajeswari Swaminathan, Yungui Huang, Caroline Astbury, Sara Fitzgerald-Butt, Katherine Miller, Justin Cole, Christopher Bartlett, Simon Lin
The increased adoption of clinical whole exome sequencing (WES) has improved the diagnostic yield for patients with complex genetic conditions. However, the informatics practice for handling information contained in whole exome reports is still in its infancy, as evidenced by the lack of a common vocabulary within clinical sequencing reports generated across genetic laboratories. Genetic testing results are mostly transmitted using portable document format, which can make secondary analysis and data extraction challenging...
May 23, 2017: Journal of the American Medical Informatics Association: JAMIA
https://www.readbyqxmd.com/read/28535026/tetanus-%C3%A2-n%C3%A3-stan-bortgl%C3%A3-md-men-allvarlig-sjukdom-%C3%A3-ldre-kvinnor-%C3%A3-r-en-riskgrupp-diagnosen-kan-vara-en-utmaning
#3
Bernhard Hozgraefe, Akad Koria, Victoria Sem, Joak Johansson
Tetanus, an almost forgotten but serious disease Tetanus is the systemic consequence of a local infection with Clostridium tetani, that produces toxin which spreads in the systemic circulation. In developed countries, it is extremely rare. Women born before 1950 are a group at risk due to lower prevalence of immunisation. This report describes a case of tetanus. The patient is a previously healthy 82-year-old woman. She presented to primary care with a complaint of stiffness in the neck and jaw. A physical exam and basic laboratory tests were normal apart from a slightly increased blood pressure...
May 22, 2017: Läkartidningen
https://www.readbyqxmd.com/read/28534892/minimizing-next-generation-sequencing-errors-for-hiv-drug-resistance-testing
#4
José A Fernández-Caballero, Natalia Chueca, Eva Poveda, Federico García
Next-generation sequencing prototypes for the routine diagnosis of resistance to antiretrovirals approved for the treatment of HIV infection are now being used in many clinical diagnostic laboratories. As some of the next-generation sequencing platforms may be a source of errors, it is necessary to improve the currently available protocols and implement bioinformatic tools that may help to correctly identify the presence of resistance mutations with clinical impact. Several studies have addressed these issues in recent years...
May 23, 2017: AIDS Reviews
https://www.readbyqxmd.com/read/28534815/multispectral-fluorescent-and-photoplethysmographic-imaging-for-remote-skin-assessment
#5
REVIEW
Janis Spigulis
Optical tissue imaging has several advantages over the routine clinical imaging methods, including non-invasiveness (it does not change the structure of tissues), remote operation (it avoids infections) and the ability to quantify the tissue condition by means of specific image parameters. Dermatologists and other skin experts need compact (preferably pocket-size), self-sustaining and easy-to-use imaging devices. The operational principles and designs of ten portable in-vivo skin imaging prototypes developed at the Biophotonics Laboratory of Institute of Atomic Physics and Spectroscopy, University of Latvia during the recent five years are presented in this paper...
May 19, 2017: Sensors
https://www.readbyqxmd.com/read/28534732/evaluation-of-adaptive-noise-management-technologies-for-school-age-children-with-hearing-loss
#6
Jace Wolfe, Mila Duke, Erin Schafer, Christine Jones, Lori Rakita
BACKGROUND: Children with hearing loss experience significant difficulty understanding speech in noisy and reverberant situations. Adaptive noise management technologies, such as fully adaptive directional microphones and digital noise reduction, have the potential to improve communication in noise for children with hearing aids. However, there are no published studies evaluating the potential benefits children receive from the use of adaptive noise management technologies in simulated real-world environments as well as in daily situations...
May 2017: Journal of the American Academy of Audiology
https://www.readbyqxmd.com/read/28534703/chlorhexidine-and-silver-sulfadiazine-coating-on-central-venous-catheters-is-not-sufficient-for-protection-against-catheter-related-infection-simulation-based-laboratory-research-with-clinical-validation
#7
Yoon Ji Choi, Jae Kwan Lim, Jeong Jun Park, Hyub Huh, Dong-Joo Kim, Chang-Hoon Gong, Seung Zhoo Yoon
Objective The efficacy of chlorhexidine- and silver sulfadiazine-coated central venous catheters (CSS-CVC) against catheter-related infection remains controversial. We hypothesized that the loss of silver nanoparticles may reduce the antibacterial efficacy of CSS-CVCs and that this loss could be due to the frictional force between the surface of the CVC and the bloodstream. The objective of this study was to investigate whether the antimicrobial effect of CSS-CVCs decreases with increasing exposure time in a bloodstream model and quantitatively assay the antimicrobial effect of CSS-CVCs compared with polyurethane and antiseptic-impregnated CVCs...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28532395/factors-associated-with-hand-joint-destruction-in-chinese-patients-with-rheumatoid-arthritis
#8
Lijuan Zhang, Jing Wang, Qiuxiang Zhang, Ting Fu, Rulan Yin, Ze Wang, Liren Li, Xianhua Wu, Zhifeng Gu
BACKGROUND: There have been no previous report of hand joint destruction prevalence in Chinese rheumatoid arthritis (RA) patients. Therefore, the aim of this study was to investigate the prevalence and potential factors of hand joint destruction among RA patients from Nantong China. In addition, we wanted to examine the differences between functional capacity, psychological status, and quality of life in patients with hand joint destruction compared to those without hand joint destruction...
May 22, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28531342/prognostic-significance-of-preoperative-neutrophilia-on-recurrence-free-survival-in-meningioma
#9
Shirin Karimi, Mana V Vyas, Lior Gonen, Raha Tabasinejad, Quinn Ostrom, Jill Barnholtz-Sloan, Suganth Suppiah, Gelareh Zadeh, Kenneth Aldape
Background: Meningioma is the most common primary intracranial tumor and recurrence is one of the important challenges in patient management. Prognostic factors for tumor recurrences in these patients especially before surgical resection are not fully characterized. Several studies have indicated an association between changes in hematologic laboratory parameters with patient outcomes in solid malignancies. We aimed to assess the association between hematological parameters and tumor recurrence in patients with meningioma...
May 20, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/28531212/epidemiological-investigation-of-the-relationship-between-common-lower-genital-tract-infections-and-high-risk-human-papillomavirus-infections-among-women-in-beijing-china
#10
Dai Zhang, Ting Li, Lei Chen, Xiaosong Zhang, Gengli Zhao, Zhaohui Liu
BACKGROUND: The incidence of lower genital tract infections in China has been increasing in recent years. The link between high-risk human papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) remains unclear. METHODS: From March to October 2014, gynecological examinations and questionnaires were conducted on 1218 married women. Cervical secretions and vaginal swab specimens were tested for Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Ureaplasma urealyticum (UU), yeast, clue cells and HR-HPV...
2017: PloS One
https://www.readbyqxmd.com/read/28531179/the-gingival-vein-as-a-minimally-traumatic-site-for-multiple-blood-sampling-in-guinea-pigs-and-hamsters
#11
Mariana Valotta Rodrigues, Simone Oliveira de Castro, Cynthia Zaccanini de Albuquerque, Vânia Gomes de Moura Mattaraia, Marcelo Larami Santoro
Laboratory animals are still necessary in scientific investigation and vaccine testing, but while novel methodological approaches are not available for their replacement, the search for new, humane, easy, and painless methods is necessary to diminish their stress and pain. When multiple blood samples are to be collected from hamsters and guinea pigs, the number of available venipuncture sites-which are greatly diminished in these species in comparison with other rodents due to the absence of a long tail-, harasses animal caregivers and researchers...
2017: PloS One
https://www.readbyqxmd.com/read/28530797/unexpected-biotransformation-of-the-hdac-inhibitor-vorinostat-yields-aniline-containing-fungal-metabolites
#12
Donovon A Adpressa, Kayla J Stalheim, Philip Jerome Proteau, Sandra Loesgen
The diversity of genetically encoded small molecules produced by filamentous fungi remains largely unexplored, which makes these fungi an attractive source for the discovery of new compounds. However, accessing their full chemical repertoire under common laboratory culture conditions is a challenge. Epigenetic manipulation of gene expression has become a well-established tool for overcoming this obstacle. Here we report that perturbation of the endophytic ascomycete Chalara sp. 6661, producer of the isofusidienol class of antibiotics, with the HDAC inhibitor vorinostat resulted in the production of four new modified xanthones...
May 22, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28530770/diagnostic-value-of-appendicular-doppler-ultrasonography-in-acute-appendicitis
#13
Hüseyin Uzunosmanoğlu, Yunsur Çevik, Şeref Kerem Çorbacıoğlu, Emine Akıncı, Hakan Buluş, Kadir Ağladıoğlu
BACKGROUND: Acute appendicitis is one of the most common causes of acute abdominal pain prompting emergency department (ED) visits. It is critical for the physicians to promptly and accurately diagnose acute appendicitis. The present study aimed to evaluate the diagnostic efficacy of Doppler ultrasonography (USG) in patients with acute appendicitis and compare this new method with other commonly used radio-diagnostic tools. METHODS: All patients who were diagnosed with acute appendicitis at the Kecioren Training and Research Hospital ED and later underwent appendectomy between October 2012 and April 2013 were included in the study...
May 2017: Ulusal Travma Ve Acil Cerrahi Dergisi, Turkish Journal of Trauma & Emergency Surgery: TJTES
https://www.readbyqxmd.com/read/28530559/meat-species-identification-by-two-direct-triplex-real-time-pcr-assays-using-low-resolution-melting
#14
Phuvadol Thanakiatkrai, Thitika Kitpipit
We developed and validated the first direct multiplex real-time PCR assay with melt curve analysis for the identification of different types of meat. The assay detects six commonly eaten meat species and provides the following benefits: ease of use, shortened analysis time, and decreased analysis cost. Species-specific primers were designed from cytochrome b, cytochrome oxidase I, and 16S rRNA genes to generate PCR products with specific melting peaks that differentiate pork, beef, horsemeat, duck meat, ostrich meat, and chicken...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530476/time-trials-versus-time-to-exhaustion-tests-effects-on-critical-power-w-and-oxygen-uptake-kinetics
#15
Bettina Karsten, Jonathan Baker, Fernando Naclerio, Andreas Klose, Antonino Bianco, Alfred Nimmerichter
PURPOSE: To investigate single-day time-to-exhaustion (TTE) and time trial (TT) based laboratory tests values of critical power (CP), Wprime (W') and respective oxygen kinetics responses. METHODS: Twelve cyclists performed a maximal ramp test followed by three TTE and three TT efforts interspersed by a 60-min recovery between efforts. Oxygen uptake was measured during all trials. The mean response time (MRT) was calculated as a description of the overall V̇O2 kinetic response from the onset to 2 min of exercise...
May 22, 2017: International Journal of Sports Physiology and Performance
https://www.readbyqxmd.com/read/28529156/transgenerational-effects-of-maternal-and-grandmaternal-age-on-offspring-viability-and-performance-in-drosophila-melanogaster
#16
Margaret C Bloch Qazi, Paige B Miller, Penny M Poeschel, Mai H Phan, Joseph L Thayer, Christian L Medrano
In non-social insects, fitness is determined by relative lifetime fertility. Fertility generally declines with age as a part of senescence. For females, senescence has profound effects on fitness by decreasing viability and fertility as well as those of her offspring. However, important aspects of these maternal effects, including the cause(s) of reduced offspring performance and carry-over effects of maternal age, are poorly understood. Drosophila melanogaster is a useful system for examining potential transgenerational effects of increasing maternal age, because of their use as a model system for studying the physiology and genetic architecture of both reproduction and senescence...
May 18, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28529048/laboratory-based-performance-evaluation-of-pima-cd4-t-lymphocyte-count-point-of-care-by-lay-counselors-in-kenya
#17
Clement Zeh, Charles E Rose, Seth Inzaule, Mitesh A Desai, Fredrick Otieno, Felix Humwa, Benta Akoth, Paul Omolo, Robert T Chen, Yenew Kebede, Taraz Samandri
BACKGROUND: CD4+ T-lymphocyte count testing at the point-of-care (POC) may improve linkage to care of persons diagnosed with HIV-1 infection, but the accuracy of POC devices when operated by lay-counselors in the era of task-shifting is unknown. We examined the accuracy of Alere's Pima™ POC device on both capillary and venous blood when performed by lay-counselors and laboratory technicians. METHODS: In Phase I, we compared the perfomance of POC against FACSCalibur™ for 280 venous specimens by laboratory technicians...
May 18, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28529026/the-use-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-for-rapid-bacterial-identification-in-patients-with-smear-positive-bacterial-meningitis
#18
Boaz Bishop, Yuval Geffen, Adi Plaut, Ola Kassis, Roni Bitterman, Mical Paul, Ami Neuberger
OBJECTIVES: To assess the potential of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in rapid identification of bacteria from smear-positive cerebrospinal fluid (CSF) in a cohort of patients with meningitis. METHODS: Single-center observational study, including adults and children with community-acquired or postneurosurgical bacterial meningitis. Meningitis was defined using established criteria. CSF samples that had a positive CSF Gram stain were directly examined by MALDI-TOF-MS...
May 18, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28528568/a-treatment-protocol-for-management-of-bacterial-and-fungal-malignant-external-otitis-a-large-cohort-in-tehran-iran
#19
Mehrdad Hasibi, Mohammadtaghi Khorsandi Ashtiani, Masoud Motassadi Zarandi, Nasrin Yazdani, Pedram Borghei, Ali Kuhi, Sasan Dabiri, Reza Hosseini, Sara Sardashti
AIMS: High rates of negative microbiologic test results highlight the potential role of empiric antimicrobial agents in management of malignant otitis externa (MOE). This study investigates the clinical presentation, laboratory findings, and response to empiric treatment in a large group of patients admitted to a tertiary academic hospital in Tehran, Iran. METHODS AND MATERIALS: We recruited 224 patients diagnosed with MOE in a prospective observation from 2009 through 2015...
May 1, 2017: Annals of Otology, Rhinology, and Laryngology
https://www.readbyqxmd.com/read/28527682/analyzing-the-experiences-of-adolescent-control-rats-effects-of-the-absence-of-physical-or-social-stimulation-on-anxiety-like-behaviour-are-dependent-on-the-test
#20
Namrata Joshi, Ronald A Leslie, Tara S Perrot
The present study was designed to systematically assess the control experience routinely used in our laboratory as part of studies on predator odour stress. Specifically, we examined effects of the physical and social components of this control experience on measures of anxiety-like behaviour in adolescent rats. Adolescent animals are at increased susceptibility to environmental perturbations and have been used for such studies much less often. Male and female Long-Evans rats were subjected to physical stimulation (Exposed or Unexposed) and social stimulation (Single Housed or Pair Housed), resulting in four groups...
May 17, 2017: Physiology & Behavior
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