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https://www.readbyqxmd.com/read/29334667/lithium-a-classic-drug-in-psychiatry-improves-nilotinib-mediated-antileukemic-effects
#1
Janaína Peixoto-da-Silva, Andrana K Calgarotto, Katiucha R Rocha, Caroline Palmeira-Dos-Santos, Soraya S Smaili, Gustavo J S Pereira, Fernando V Pericole, Adriana da Silva S Duarte, Sara T O Saad, Claudia Bincoletto
Although Tyrosine kinase inhibitors (TKIs) that target Bcr-Abl play a key role in Chronic Myeloid Leukemia (CML) therapy, they do not eradicate CML-initiating cells, which lead to the emergence of drug resistance. Here we used the lithium, a GSK-3 inhibitor, to attempt to potentiate the effects of nilotinib against leukemia cells. For this purpose, a K562 leukemia cell line and bone marrow cells from untreated Chronic Myeloid Leukemia (CML) patients, prior to any exposure to TKIs, were used as a model. Our results demonstrated that the combination of lithium + nilotinib (L + N) induced K562-cell death and cleaved caspase-3 when compared to lithium or nilotinib alone, accompanied by GSK-3β phosphorylation and Bcr-Abl oncoprotein levels reduction...
January 12, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29334649/determining-variables-that-influence-the-phosphorus-content-of-waste-stabilization-pond-algae
#2
Matthew D Sells, Nicola Brown, Andrew N Shilton
Waste stabilization ponds (WSP) are one of the most common forms of wastewater treatment for smaller communities globally, but have poor phosphorus removal. It is known that WSP algae can accumulate polyphosphate within their cells in excess of that needed for cell function. If polyphosphate accumulation could be triggered at the higher range of WSP cell concentrations, phosphorus removal from domestic wastewater could be significantly improved. However, this phenomenon is sporadic and still not fully understood...
January 8, 2018: Water Research
https://www.readbyqxmd.com/read/29334476/biological-control-of-botrytis-cinerea-interactions-with-native-vineyard-yeasts-from-washington-state
#3
Xuefei Wang, Dean A Glawe, Elizabeth K Kramer, David M Weller, Patricia Ann Okubara
Native yeasts are of increasing interest to researchers, grape growers, and vintners because of their potential for biocontrol activity, and their contributions to the aroma, flavor and mouthfeel qualities of wines. To assess biocontrol activity, we tested 11 yeasts from Washington vineyards, representing isolates of Candida saitoana, Curvibasidium pallidicorallinum, Metschnikowia chrysoperlae, Metschnikowia pulcherrima, Meyerozyma guilliermondii, Saccharomyces cerevisiae and Wickerhamomyces anomalus, for ability to colonize 'Thompson Seedless' grape berries, inhibit the growth of Botrytis cinerea in vitro, and suppress disease symptoms on isolated berries...
January 15, 2018: Phytopathology
https://www.readbyqxmd.com/read/29334295/neuritin-provides-neuroprotection-against-experimental-traumatic-brain-injury-in-rats
#4
Qi Liu, Hang Zhang, Jian Xu, Dong Zhao
Objectives Traumatic brain injury (TBI) is a leading cause of death and disability worldwide. Neuritin is a neurotrophic factor that regulates neural growth and development. However, the role of neuritin in alleviating TBI has not been investigated. Methods In this study, Sprague Dawley rats (n = 144) weighing 300 ± 50 g were categorized into control, sham, TBI, and TBI + neuritin groups. The neurological scores and the ultrastructure of cortical neurons, apoptotic cells, and caspase-3 were measured by using Garcia scoring system, transmission electron microscopy, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, Western blot analysis, and real-time RT-PCR at various time points post-TBI...
January 15, 2018: International Journal of Neuroscience
https://www.readbyqxmd.com/read/29334260/crude-extract-of-origanum-vulgare-l-induced-cell-death-and-suppressed-mapk-and-pi3-akt-signaling-pathways-in-sw13-and-h295r-cell-lines
#5
Beatrice Rubin, Jacopo Mansi, Halenya Monticelli, Loris Bertazza, Marco Redaelli, Francesca Sensi, Maira Zorzan, Carla Scaroni, Caterina Mian, Maurizio Iacobone, Decio Armanini, Cristina Bertolini, Susi Barollo, Marco Boscaro, Raffaele Pezzani
Oregano (Origanum vulgare L.) is a common aromatic plant used in Mediterranean and Asian Regions for treating respiratory diseases, painful menstruation, rheumatoid arthritis, etc. Recently its role as an anticancer plant has been suggested, although oregano has been never evaluated into adrenocortical tumour cell models. This study analysed for the first time the anticancer effects of a crude extract of wild mountain oregano (Origanum vulgare L.) in SW13 and H295R cell lines. The crude extract was characterised by GC/MS and the toxic effects of oregano were first analysed by brine shrimp lethality assay...
January 15, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29334187/surveying-the-landscape-of-optogenetic-methods-for-detection-of-protein-protein-interactions
#6
REVIEW
Matthew D Wiens, Robert E Campbell
Mapping the protein-protein interaction (PPi) landscape is of critical importance to furthering our understanding how cells and organisms function. Optogenetic methods, that is, approaches that utilize genetically encoded fluorophores or fluorogenic enzyme reactions, uniquely enable the visualization of biochemical phenomena in live cells with high spatial and temporal accuracy. Applying optogenetic methods to the detection of PPis requires the engineering of protein-based systems in which an optical signal undergoes a substantial change when the two proteins of interest interact...
January 15, 2018: Wiley Interdisciplinary Reviews. Systems Biology and Medicine
https://www.readbyqxmd.com/read/29334179/functionalized-nanoparticles-efficiently-enhancing-the-targeted-delivery-tumor-penetration-and-anticancer-activity-of-7-ethyl-10-hydroxycamptothecin
#7
Xun Liu, Jingxing Si, Qianzhi Zhang, Qian Huang, Danxia Gu, Hao Yang, Xu Chen, Youqing Shen, Meihua Sui
The enhanced permeability and retention (EPR) effect of tumors is much more complex than initially defined, and it alone is not sufficient for targeted delivery of nanosized agents. Meanwhile, poor tumor penetration is another major challenge for the treatment of solid tumors using nanoparticles. Development of delivery systems for SN38, the active metabolite of CPT-11 in human and a very potent anticancer molecule, has become an attractive research area. PEGx -p(HEMASN38)y (x and y are viable), a prodrug synthesized by using polyethylene glycol (PEG) as initiator and SN38 as monomer through atom transfer radical polymeration (ATRP) method, is previously reported...
January 15, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29334092/synthetic-cells-produce-a-quorum-sensing-chemical-signal-perceived-by-pseudomonas-aeruginosa
#8
Giordano Rampioni, Francesca D'Angelo, Marco Messina, Alessandro Zennaro, Yutetsu Kuruma, Daniela Tofani, Livia Leoni, Pasquale Stano
Recent developments in bottom-up synthetic biology (e.g., lipid vesicle technology integrated with cell-free protein expression systems) allow the generation of semi-synthetic minimal cells (in short, synthetic cells, SCs) endowed with some distinctive capacities of natural cells. In particular, such approaches provide technological tools and conceptual frameworks for the design and engineering of programmable SCs capable of communicating with natural cells by exchanging chemical signals. Here we describe the generation of giant vesicle-based SCs which, via gene expression, synthesize in their aqueous lumen an enzyme that in turn produces a chemical signal...
January 15, 2018: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29333942/six1-and-dach1-influence-the-proliferation-and-apoptosis-of-hepatocellular-carcinoma-through-regulating-p53
#9
Qi Cheng, Deng Ning, Jin Chen, Xue Li, Xiaoping Chen, Li Jiang
ABSTRACTS This research aimed to explore effects of SIX1 and DACH1 on hepatocellular carcinoma (HCC) cell proliferation, apoptosis and cell cycle. Fifty paired hepatocellular carcinoma tissues were screened for differentially expressed genes. SIX1 and DACH1 expressions were subjected to qRT-PCR and western blot in tumor tissues and cells. The knockdown efficiency of siRNAs and transfection efficiency of cDNAs and siRNAs were validated by qRT-PCR and western blot as well. Then colony formation assay and flow cytometry were applied to observe cell proliferation, cell apoptosis and cell cycle changes...
January 15, 2018: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/29333781/-research-progress-on-the-relationship-between-triggering-receptor-expressed-on-myeloid-cells-1-and-2-and-malignant-tumors
#10
Yang Yun, Wu Wuchao, Wu Yafei, Zhao Lei
Increasing scientific evidence supports the positive relationship between inflammation and cancer development. The immune response initiated by pattern recognition receptors is critical to triggering of tumor-associated inflammation. Triggering receptor expressed on myeloid cells (TREM) is an immunoglobulin of the super transmembrane glycoprotein family, which is mainly expressed on select groups of myeloid cells. The most important members of TREM comprise TREM-1 and TREM-2. Activation of TREM-1 and TREM-2 signaling is initiated upon binding of their ligands...
December 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29333769/-significance-of-the-expression-of-proliferating-cell-nuclear-antigen-and-p53-in-the-regeneration-process-of-an-atrophic-parotid-gland
#11
Wei Yueduan, Zuo Jinhua, Ding Changling, Zhu Yuhong, Wang Lifang, Song Bing, Wang Jing
OBJECTIVE: This research aims to further explore the expression and significance of proliferating cell nuclear antigen (PCNA) and P53 in regenerating rat atrophy parotid gland from the gene and protein levels. METHODS: One hundred and two Wistar rats were randomly divided into experimental and control groups; the former group's duct was ligated and then released respectively in 7 (Group A) and 14 days (Group B). Fresh parotid specimens were obtained at 0, 3, 5, 7, 10, 14, 21, and 28 days after being released...
December 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29333754/a-guide-to-analysis-and-reconstruction-of-serial-block-face-scanning-electron-microscopy-data
#12
E Cocks, M Taggart, F C Rind, K White
Serial block face scanning electron microscopy (SBF-SEM) is a relatively new technique that allows the acquisition of serially sectioned, imaged and digitally aligned ultrastructural data. There is a wealth of information that can be obtained from the resulting image stacks but this presents a new challenge for researchers - how to computationally analyse and make best use of the large datasets produced. One approach is to reconstruct structures and features of interest in 3D. However, the software programmes can appear overwhelming, time-consuming and not intuitive for those new to image analysis...
January 15, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29333657/gelatin-methacryloyl-hydrogels-in-the-absence-of-a-crosslinker-as-3d-glioblastoma-multiforme-gbm-mimetic-microenvironment
#13
Pelin Erkoc, Fidan Seker, Tugba Bagci-Onder, Seda Kizilel
3D platforms are important for monitoring tumor progression and screening drug candidates to eradicate tumors such as glioblastoma multiforme (GBM), a malignant type of human brain tumor. Here, a new strategy is reported that exploits visible-light-induced crosslinking of gelatin where the reaction is carried out in the absence of an additional crosslinker. Visible light-induced crosslinking promotes the design of cancer microenvironment-mimetic system without compromising the cell viability during the process and absence of crosslinker facilitates the synthesis of the unique construct...
January 15, 2018: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29333114/carcinogenesis-induced-by-low-dose-radiation
#14
Igor Piotrowski, Katarzyna Kulcenty, Wiktoria Maria Suchorska, Agnieszka Skrobała, Małgorzata Skórska, Marta Kruszyna-Mochalska, Anna Kowalik, Weronika Jackowiak, Julian Malicki
Background: Although the effects of high dose radiation on human cells and tissues are relatively well defined, there is no consensus regarding the effects of low and very low radiation doses on the organism. Ionizing radiation has been shown to induce gene mutations and chromosome aberrations which are known to be involved in the process of carcinogenesis. The induction of secondary cancers is a challenging long-term side effect in oncologic patients treated with radiation. Medical sources of radiation like intensity modulated radiotherapy used in cancer treatment and computed tomography used in diagnostics, deliver very low doses of radiation to large volumes of healthy tissue, which might contribute to increased cancer rates in long surviving patients and in the general population...
December 2017: Radiology and Oncology
https://www.readbyqxmd.com/read/29333089/lentivirus-mediated-shrna-targeting-cnn2-inhibits-hepatocarcinoma-in-vitro-and-in-vivo
#15
Xueqing Kang, Feng Wang, Xiuwan Lan, Xiaolong Li, Shunxin Zheng, Zhilue Lv, Yuan Zhuang, Yongxiang Zhao, Sufang Zhou
Objective: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with a high rate of mortality. Our previous study shows the expression of calponin 2 (CNN2) is up-regulated in hepatocellular carcinoma tissues, especially in metastatic ones. To better understand the role of CNN2 in HCC, RNA interference (RNAi) was used to explore its role in tumor growth and metastasis. Methods: Lentivirus-mediated CNN2-shRNA was transfected into SK-hep-1 cells, and the efficacy of CNN2 expression, cell migration, invasion, proliferation and cell cycles were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot (WB), Transwell assay, methyl thiazol tetrazolium assay and flow cytometry, respectively...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29333086/ethical-and-safety-issues-of-stem-cell-based-therapy
#16
REVIEW
Vladislav Volarevic, Bojana Simovic Markovic, Marina Gazdic, Ana Volarevic, Nemanja Jovicic, Nebojsa Arsenijevic, Lyle Armstrong, Valentin Djonov, Majlinda Lako, Miodrag Stojkovic
Results obtained from completed and on-going clinical studies indicate huge therapeutic potential of stem cell-based therapy in the treatment of degenerative, autoimmune and genetic disorders. However, clinical application of stem cells raises numerous ethical and safety concerns. In this review, we provide an overview of the most important ethical issues in stem cell therapy, as a contribution to the controversial debate about their clinical usage in regenerative and transplantation medicine. We describe ethical challenges regarding human embryonic stem cell (hESC) research, emphasizing that ethical dilemma involving the destruction of a human embryo is a major factor that may have limited the development of hESC-based clinical therapies...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29333084/effects-of-%C3%AE-mangostin-on-viability-growth-and-cohesion-of-multicellular-spheroids-derived-from-human-breast-cancer-cell-lines
#17
Giuseppe Scolamiero, Claudia Pazzini, Francesca Bonafè, Carlo Guarnieri, Claudio Muscari
Background: α-Mangostin (αMG) is extracted from Garcinia mangostana Linn and exerts antiproliferative activities. Although several researches on αMG were performed using cell monolayers, the in vitro pharmacological effects on 3D cancer models have never been investigated. Aim of the present study was to find new anticancer properties of αMG by evaluating the changes that this compound provokes in multicellular tumour spheroids (MCTSs). Methods: MCTSs were generated from MDA-MB-231 and MCF-7 breast tumour cell lines and then treated with 0...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29333045/in-vitro-antiproliferative-effect-of-earthworm-coelomic-fluid-of-eudrilus-eugeniae-eisenia-foetida-and-perionyx-excavatus-on-squamous-cell-carcinoma-9-cell-line-a-pilot-study
#18
Dominic Augustine, Roopa S Rao, Jayaraman Anbu, K N Chidambara Murthy
Introduction: The earthworm coelomic fluid (ECF) has shown proven antiproliferative effect against breast, liver, gastrointestinal, and brain cancer, but it is least explored in oral cancer. The present in vitro study is an attempt to investigate the antiproliferative activity of ECF on oral cancer cell line squamous cell carcinoma (SCC)-9. Materials and Methods: ECF was collected from the species Eudrilus eugeniae (EE), Eisenia foetida (EF), and Perionyx excavatus (PE) stored at -80°C...
December 2017: Pharmacognosy Research
https://www.readbyqxmd.com/read/29332841/improved-neuron-culture-using-scaffolds-made-of-three-dimensional-pdms-micro-lattices
#19
Sisi Li, Francesco Paolo Ulloa Severino, Jelena Ban, Li Wang, Giulietta Pinato, Vincent Torre, Yong Chen
Tissue engineering strives to create functional components of organs with different cell types in vitro. One of the challenges is to fabricate scaffolds for three-dimensional (3D) cell culture under physiological conditions. Of particular interesting is to investigate the morphology and function of the central nervous system (CNS) cultured using such scaffolds. Here, we used an elastomer, polydimethylsiloxane (PDMS), to produce lattice-type scaffolds from a photolithography defined template. The photomask with antidot arrays was spin-coated by a thick layer of resist and downward mounted on a rotating stage at angle of 45°...
January 15, 2018: Biomedical Materials
https://www.readbyqxmd.com/read/29332642/current-affairs-future-perspectives-of-tuberculosis-and-antitubercular-agents
#20
REVIEW
Jineetkumar Gawad, Chandrakant Bonde
Tuberculosis (TB) is the major threat for humans from past several decades. Even after advent of several antitubercular drugs, researchers are still struggling for the mycobacterial infections in humans are TB and leprosy. Chronic infections caused by Mycobacterium tuberculosis and Mycobacterium leprae. A particular problem with both of these organisms is that they can survive inside macrophages after phagocytosis, unless these cells are activated by cytokines produced by T-lymphocytes, because of this researchers are not yet succeeded in finding effective treatment on TB...
January 2018: Indian Journal of Tuberculosis
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