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https://www.readbyqxmd.com/read/28743038/physiological-vascular-and-nanomechanical-assessment-of-hybrid-poplar-leaf-traits-in-micropropagated-plants-and-plants-propagated-from-root-cuttings-a-contribution-to-breeding-programs
#1
Jaroslav Ďurkovič, Hana Husárová, Lucia Javoříková, Ingrid Čaňová, Miriama Šuleková, Monika Kardošová, Ivan Lukáčik, Miroslava Mamoňová, Rastislav Lagaňa
Micropropagated plants experience significant stress from rapid water loss when they are transferred from an in vitro culture to either greenhouse or field conditions. This is caused both by inefficient stomatal control of transpiration and the change to a higher light intensity and lower humidity. Understanding the physiological, vascular and biomechanical processes that allow micropropagated plants to modify their phenotype in response to environmental conditions can help to improve both field performance and plant survival...
July 13, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28742927/a-new-model-for-netrin1-in-commissural-axon-guidance
#2
REVIEW
Daniel Morales
Now-classic experiments characterized netrin1 as a major player in commissural axon guidance in the spinal cord. The data suggest a chemotactic model in which netrin1 expression in the floor plate forms a concentration gradient that attracts commissural axons. New research published independently in Neuron and in Nature tests this model by deleting netrin1 specifically in the floor plate. Surprisingly, these conditional mutant mice have no overt commissure defects. The authors report that netrin1 decorates the pial surface of the spinal cord and hindbrain, likely deposited by radial processes of progenitor cells in the ventricular zone...
July 25, 2017: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/28742884/reduced-expression-of-e-cadherin-and-increased-sialylation-level-in-clear-cell-renal-cell-carcinoma
#3
Małgorzata Borzym-Kluczyk, Iwona Radziejewska, Marzanna Cechowska-Pasko, Barbara Darewicz
Cancer cells are characterized by an aberrant increase in protein N-glycosylation and by disruption of E-cadherin-mediated adherens junctions. However, the relationship between alterations in N-glycosylation process and loss of E-cadherin adhesion in cancer remains unclear. The mechanisms of altered expression of adhesive glycoproteins in cancer cells have not been fully elucidated. Thus, the aim of this study was to examine the expression of E-cadherin and sialyl Lewis(a)/(x), NeuAcα2-3Gal, NeuAcα2-6Gal/GalNAc structures in the normal renal tissue and intermediate and cancerous tissues from patients with clear cell RCC...
July 24, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28742850/genes-cells-and-biobanks-yes-there-s-still-a-consent-problem
#4
Timothy Caulfield, Blake Murdoch
From a research perspective, the interest in biobanking continues to intensify. Governments and industry have invested heavily in biobanks, as exemplified by initiatives like the United Kingdom Biobank and United States' Precision Medicine Initiative. But despite this enthusiasm, many profound legal and ethical challenges remain unresolved. Indeed, there continues to be disagreements about how best to obtain consent and the degree and nature of control that research participants retain over donated samples and health information...
July 2017: PLoS Biology
https://www.readbyqxmd.com/read/28742821/evolution-of-the-camp-dependent-protein-kinase-pka-catalytic-subunit-isoforms
#5
Kristoffer Søberg, Line Victoria Moen, Bjørn Steen Skålhegg, Jon Kristen Laerdahl
The 3',5'-cyclic adenosine monophosphate (cAMP)-dependent protein kinase, or protein kinase A (PKA), pathway is one of the most versatile and best studied signaling pathways in eukaryotic cells. The two paralogous PKA catalytic subunits Cα and Cβ, encoded by the genes PRKACA and PRKACB, respectively, are among the best understood model kinases in signal transduction research. In this work, we explore and elucidate the evolution of the alternative 5' exons and the splicing pattern giving rise to the numerous PKA catalytic subunit isoforms...
2017: PloS One
https://www.readbyqxmd.com/read/28742685/recurrence-patterns-and-long-term-results-after-induction-chemotherapy-chemoradiotherapy-and-curative-surgery-in-patients-with-locally-advanced-esophageal-cancer
#6
Thomas Steffen, Daniel Dietrich, Annelies Schnider, Christoph Kettelhack, Olivier Huber, Walter R Marti, Markus Furrer, Beat Gloor, Marc Schiesser, Sandra Thierstein, Peter Brauchli, Thomas Ruhstaller
OBJECTIVE: The long-term follow up data of 2 prospective phase II trials is reported (NCT00072033, NCT00445861), which investigated neoadjuvant chemoradiation followed by surgery in patients with esophageal carcinoma. Postoperative complications as well as prognostic factors and patterns of relapse during long-term observation are shown. SUMMARY OF BACKGROUND DATA: Long-term follow-up is often missing in the complex setting of multimodal treatments of esophageal carcinoma; this leads to rather undifferentiated follow-up guidelines for this tumor entity...
July 24, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/28742212/relevant-researches-on-chronic-viral-myocarditis-cvmc-in-children-complicated-with-arrhythmia-and-thyroid-hormone-level
#7
M-Y Fu, Q-W Wang, Y Xue, F Xu, C-L Li, X-J An
OBJECTIVE: To analyze the correlation between chronic viral myocarditis (CVMC) in children, complicated with arrhythmia and thyroid hormone level. PATIENTS AND METHODS: 60 patients with CVMC complicated with arrhythmia were continuously selected (course of disease > 3 months) and they are were diagnosed with arrhythmia by the routine 18-lead electrocardiogram and 24-hour Holter; the average follow-up time is about 2 years, during which the left ventricular end-diastolic diameter (LVEDd), left ventricular ejection fraction (LVEF), the occurrence rate of malignant arrhythmia events, immune state of T cell and thyroid hormone level (FT3, FT4, TSH, TGAb and TPOAb) were compared...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28742190/the-research-on-the-influences-of-hyperthermal-perfusion-chemotherapy-combined-with-immunologic-therapy-on-the-immunologic-function-and-levels-of-circulating-tumor-cells-of-the-advanced-colorectal-cancer-patients-with-liver-metastasis
#8
J-J Sun, G-L Fan, X-G Wang, K Xu
OBJECTIVE: To investigated the influence of hyperthermal perfusion chemotherapy combined with immunologic therapy on the immunologic function and levels of circulating tumor cells of the advanced colorectal cancer patients with liver metastasis. PATIENTS AND METHODS: We enrolled 98 advanced colorectal cancer patients with liver metastasis that were admitted to this hospital for treatment and were randomly divided into two groups, the observation group (n = 49) and the control group (n = 49)...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28741837/immunological-and-virological-response-to-antiretroviral-treatment-in-migrant-and-native-men-and-women-in-western-europe-is-benefit-equal-for-all
#9
(no author information available yet)
OBJECTIVES: The aim of the study was to evaluate differences in immunovirological response to combination antiretroviral therapy (cART) in migrant and native men and women within a European collaboration of HIV cohorts Collaboration of Observational HIV Epidemiological Research in Europ (COHERE) in EuroCoord, 2004-2013. METHODS: Migrants were defined as those with geographical origin (GO) different from the reporting country and were grouped as originating from Western Europe and Western Countries (WEWC), Eastern Europe (EE), North Africa and the Middle East (NAME), sub-Saharan Africa (SSA), Latin America (LA), Caribbean (CRB) and Asia/Oceania (ASIA/OCE)...
July 25, 2017: HIV Medicine
https://www.readbyqxmd.com/read/28741688/new-insights-into-naevoid-melanomas-a-clinico-pathological-reassessment
#10
Martin G Cook, Daniela Massi, Willeke A M Blokx, Joost Van den Oord, Senada Koljenović, Vincenzo De Giorgi, Eleanor Kissin, Megan Grant, Amit Mandal, Gabriela Gremel, Caroline Gaudy, Amaya Viros, Nathalie Dhomen, Kiarash Khosrotehrani, Richard Marais, Adele C Green, Martin C Mihm
AIMS: Because the term 'naevoid melanoma' has variable clinical and pathologic interpretations, we aimed to clarify the features of melanomas referred to as naevoid. METHODS AND RESULTS: A review was undertaken of 102 melanomas diagnosed histopathologically as naevoid melanomas and ascertained by European Organisation for Research and Treatment of Cancer Melanoma Group Subcommittee pathologists from their records. We found these could be classified morphologically into 3 groups...
July 25, 2017: Histopathology
https://www.readbyqxmd.com/read/28741596/the-response-of-mri-contrast-parameters-in-i-in-vitro-i-tissues-and-tissue-mimicking-phantoms-to-fractionation-by-histotripsy
#11
Steven Paul Allen, Eli Vlaisavljevich, Jiaqi Shi, Luis Hernandez-Garcia, Charles A Cain, Zhen Xu, Timothy L Hall
Histotripsy is a non-invasive, focused ultrasound lesioning technique that can ablate precise volumes of soft tissue using a novel mechanical fractionation mechanism. Previous research suggests that magnetic resonance imaging (MRI) may be a sensitive image-based feedback mechanism for histotripsy. However, there are insufficient data to form some unified understanding of the response of the MR contrast mechanisms in tissues to histotripsy. In this paper, we investigate the response of the MR contrast parameters R1, R2, and the apparent diffusion coefficient (ADC) to various treatment levels of histotripsy in in-vitro porcine liver, kidney, muscle, and blood clot as well in formulations of bovine red blood cells suspended in agar gel...
July 25, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28741507/mtor-signaling-confers-resistance-to-targeted-cancer-drugs
#12
REVIEW
Yakir Guri, Michael N Hall
Cancer is a complex disease and a leading cause of death worldwide. Extensive research over decades has led to the development of therapies that target cancer-specific signaling pathways. However, the clinical benefits of such drugs are at best transient due to tumors displaying intrinsic or adaptive resistance. The underlying compensatory pathways that allow cancer cells to circumvent a drug blockade are poorly understood. We review here recent studies suggesting that mammalian TOR (mTOR) signaling is a major compensatory pathway conferring resistance to many cancer drugs...
November 2016: Trends in Cancer
https://www.readbyqxmd.com/read/28741454/nanovesicular-formulations-for-cancer-gene-therapy
#13
Lorena Tavano, Elisabetta Mazzotta, Rita Muzzalupo
In the last decades, gene therapy has become a novel therapeutic strategy for cancer treatment, including immunologic and molecular approaches. Among molecular avenue, the design of efficient and effective gene delivery systems, like cationic liposomes and niosomes, has been widely investigated and proposed as the most promising research area. The advantages of cationic vesicles rely on their natural ability to form complexes with anionic genetic molecules and deliver them into the cells via the endosomal pathway...
May 18, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28741379/tiny-rnas-and-their-voyage-via-extracellular-vesicles-secretion-of-bacterial-small-rna-and-eukaryotic-microrna
#14
Ji-Woong Choi, Jee-Hyun Um, Jin-Hyun Cho, Heon-Jin Lee
MicroRNAs are small non-coding RNAs that bind to the 3'-untranslated region of target mRNAs and have transcriptional or translational inhibitory function in eukaryotes. Before microRNAs were widely known, bacterial non-coding small RNAs around 50-200 nt in length were discovered whose mechanism of action resembled that of microRNAs. Recently, RNAs that are of similar size to or smaller than microRNAs have been discovered in bacteria and indeed, this class of small RNAs have been found throughout all domains of life...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28741203/flow-cytometric-characterization-of-bacterial-abundance-and-physiological-status-in-a-nitrifying-denitrifying-activated-sludge-system-treating-landfill-leachate
#15
Sergio Collado, Paula Oulego, Saúl Alonso, Mario Díaz
Flow cytometry has recently been presented as a research tool in the assessment of the viability/activity of activated sludge from municipal wastewater treatment plants, but it has not put in practice for industrial biotreatments yet. In this study, for the first time ever, the reliability and significance of the multiparameter flow cytometry applied to the biological nitrification-denitrification treatment of leachate have been evaluated. Using a double staining procedure (cFDA/PI), the viable, damaged, and dead subpopulations were determined, and the results were compared to those obtained with conventional methods, such as nitrogen and oxygen uptake rates or plate counting...
July 24, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28741063/effects-of-astragalus-polysaccharide-on-mechanical-characterization-of-liver-sinusoidal-endothelial-cells-by-atomic-force-microscopy-at-nanoscale
#16
Wen-Liang Lu, Juan-Mei Li, Jiao Yang, Chen-Guang Xu, Sha-Sha Zhang, Jie Yan, Ting-Ting Zhang, Hui-Hui Zhao
OBJECTIVE: To study the effects of Astragalus polysaccharide (APS), the primary effective component of the Chinese herb medicine Astragalus membranaceus (frequently used for its anti-hepatic fibrosis effects), on nanoscale mechanical properties of liver sinusoidal endothelial cells (SECs). METHODS: Using endothelial cell medium as the control, 5 experimental groups were established utilizing different concentrations of APS, i.e. 12.5, 25, 50, 100, and 200 μg/mL...
July 25, 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28740830/structure-activity-regulation-and-role-of-the-mitochondrial-calcium-uniporter-in-health-and-disease
#17
REVIEW
Cristina Mammucari, Gaia Gherardi, Rosario Rizzuto
Mitochondrial Ca(2+) uptake plays a pivotal role both in cell energy balance and in cell fate determination. Studies on the role of mitochondrial Ca(2+) signaling in pathophysiology have been favored by the identification of the genes encoding the mitochondrial calcium uniporter (MCU) and its regulatory subunits. Thus, research carried on in the last years on one hand has determined the structure of the MCU complex and its regulation, on the other has uncovered the consequences of dysregulated mitochondrial Ca(2+) signaling in cell and tissue homeostasis...
2017: Frontiers in Oncology
https://www.readbyqxmd.com/read/28740732/low-molecular-weight-ngf-mimetic-corrects-the-cognitive-deficit-and-depression-like-behavior-in-experimental-diabetes
#18
R U Ostrovskaya, S S Yagubova, T A Gudasheva, S B Seredenin
Based on the comorbidity of diabetes, depression, and dementia and recognizing that a deficiency of the nerve growth factor (NGF) is involved in all of these kinds of pathologies, we studied the effect of the mimetic of dimeric dipeptide NGF loop 4, GK-2, on a model of streptozotocin-induced type 2 diabetes in C57Bl/6 mice. GK-2 [hexamethylenediamide bis-(N-monosuccinyl-glutamyl-lysine)] was synthesized at the V.V. Zakusov Scientific Research Institute of Pharmacology. The study revealed the ability of GK-2 to ameliorate hyperglycemia induced by streptozotocine (STZ 100 mg/kg i...
April 2017: Acta Naturae
https://www.readbyqxmd.com/read/28740639/rgd-peptide-and-graphene-oxide-co-functionalized-plga-nanofiber-scaffolds-for-vascular-tissue-engineering
#19
Yong Cheol Shin, Jeonghyo Kim, Sung Eun Kim, Su-Jin Song, Suck Won Hong, Jin-Woo Oh, Jaebeom Lee, Jong-Chul Park, Suong-Hyu Hyon, Dong-Wook Han
In recent years, much research has been suggested and examined for the development of tissue engineering scaffolds to promote cellular behaviors. In our study, RGD peptide and graphene oxide (GO) co-functionalized poly(lactide-co-glycolide, PLGA) (RGD-GO-PLGA) nanofiber mats were fabricated via electrospinning, and their physicochemical and thermal properties were characterized to explore their potential as biofunctional scaffolds for vascular tissue engineering. Scanning electron microscopy images revealed that the RGD-GO-PLGA nanofiber mats were readily fabricated and composed of random-oriented electrospun nanofibers with average diameter of 558 nm...
June 2017: Regenerative Biomaterials
https://www.readbyqxmd.com/read/28740612/3-hydroxykynurenine-a-tryptophan-metabolite-generated-during-the-infection-is-active-against-trypanosoma-cruzi
#20
Carolina P Knubel, Constanza Insfran, Fernando F Martinez, Cintia Diaz Lujan, Ricardo E Fretes, Martin G Theumer, Laura Cervi, Claudia C Motran
The antiparasitic activity of 3-hydroxykynurenine (3-HK), one of the major tryptophan catabolites of the kynurenine pathway, against both Trypanosoma cruzi evolutive forms that are important for human infection, trypomastigotes (Tps) and amastigotes (Am), possible targets in the parasite and the drug toxicity to mammalian cells have been investigated. 3-HK showed a potent activity against Am with IC50 values in the micromolar concentration range, while the IC50 values to cause Tps death was ∼6000-times higher, indicating that the replicative form present in the vertebrate hosts is much more susceptible to 3-HK than bloodstream Tps...
July 13, 2017: ACS Medicinal Chemistry Letters
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