keyword
MENU ▼
Read by QxMD icon Read
search

Enterococcus faecalis

keyword
https://www.readbyqxmd.com/read/29348921/genetic-characteristics-of-vana-type-vancomycin-resistant-enterococcus-faecalis-and-enterococcus-faecium-in-cuba
#1
D Quiñones, M S Aung, J P Sousa Martins, N Urushibara, N Kobayashi
VanA-type vancomycin-resistant enterococci isolates from bloodstream infections in Cuba were genetically characterized. Enterococcus faecalis isolates were assigned to sequence type (ST) 28, closely related to Eastern Europe, while Enterococcus faecium belonged to ST262, ST656 and ST1349, and showed different genetic profiles.
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29346643/genetic-characterization-of-a-vang-type-vancomycin-resistant-enterococcus-faecium-clinical-isolate
#2
Mohamed Sassi, François Guérin, Léonie Lesec, Christophe Isnard, Marguerite Fines-Guyon, Vincent Cattoir, Jean-Christophe Giard
Objectives: To characterize, phenotypically and genotypically, the first Enterococcus faecium clinical isolate harbouring a vanG operon. Methods: The antibiotic resistance profile of E. faecium 16-346 was determined and its whole genome sequenced using PacBio technology. Attempts to transfer vancomycin resistance by filter mating were performed and the inducibility of expression of the vanG operon was studied by reverse-transcription quantitative PCR (RT-qPCR) in the presence or absence of subinhibitory concentrations of vancomycin...
January 16, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29346325/synthesis-and-antimicrobial-activity-of-4-substituted-1-2-3-triazole-coumarin-derivatives
#3
Priscila López-Rojas, Monika Janeczko, Konrad Kubiński, Ángel Amesty, Maciej Masłyk, Ana Estévez-Braun
A new series of coumarin-1,2,3-triazole conjugates with varied alkyl, phenyl and heterocycle moieties at C-4 of the triazole nucleus were synthesized using a copper(I)-catalysed Huisgen 1,3-dipolar cycloaddition reaction of corresponding O-propargylated coumarin (3) or N-propargylated coumarin (6) with alkyl or aryl azides. Based on their minimal inhibitory concentrations (MICs) against selected microorganisms, six out of twenty-six compounds showed significant antibacterial activity towards Enterococcus faecalis (MIC = 12...
January 18, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29337627/increased-incidence-of-enterococcal-infection-in-nonviable-broiler-chicken-embryos-in-western-canadian-hatcheries-as-detected-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#4
Ruwani Karunarathna, Shelly Popowich, Morgan Wawryk, Betty Chow-Lockerbie, Khawaja Ashfaque Ahmed, Chenfang Yu, Mengying Liu, Kalhari Goonewardene, Thushari Gunawardana, Shanika Kurukulasuriya, Ashish Gupta, Philip Willson, Neil Ambrose, Musangu Ngeleka, Susantha Gomis
The emergence of enterococcal infections in neonatal broiler chickens in the poultry industry has become common in many countries, including Canada. The objective of this study was to examine the bacterial infections in nonviable broiler chicken embryos in three western Canadian poultry hatcheries using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The pattern of embryo mortality that occurred during incubation and the breakout analysis results were similar in all three hatcheries...
December 2017: Avian Diseases
https://www.readbyqxmd.com/read/29337343/antimicrobial-resistance-genetic-markers-in-potentially-pathogenic-gram-positive-cocci-isolated-from-brazilian-soft-cheese
#5
Juliana Alves Resende, Cláudia Oliveira Fontes, Alessandra Barbosa Ferreira-Machado, Thiago César Nascimento, Vânia Lúcia Silva, Cláudio Galuppo Diniz
Although most Brazilian dairy products meet high technological standards, there are quality issues regarding milk production, which may reduce the final product quality. Several microbial species may contaminate milk during manufacture and handling. If antimicrobial usage remains uncontrolled in dairy cattle, the horizontal transfer of antimicrobial resistance genes in foodstuffs may be of particular concern for both food producers and dairy industry. This study focused on the evaluation of putative Gram positive cocci in Minas cheese and of antimicrobial and biocide resistance genes among the isolated bacteria...
January 16, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29337224/an-experimental-study-for-rapid-detection-and-quantification-of-endodontic-microbiota-following-photo-activated-disinfection-via-new-multiplex-real-time-pcr-assay
#6
Maryam Pourhajibagher, Reza Raoofian, Roghayeh Ghorbanzadeh, Abbas Bahador
BACKGROUND: The infected root canal system harbors one of the highest accumulations of polymicrobial infections. Since the eradication of endopathogenic microbiota is a major goal in endodontic infection therapy, photo-activated disinfection (PAD) can be used as an alternative therapeutic method in endodontic treatment. Compared to cultivation-based approaches, molecular techniques are more reliable for identifying microbial agents associated with endodontic infections. The purpose of this study was to evaluate the ability of designed multiplex real-time PCR protocol for the rapid detection and quantification of six common microorganisms involved in endodontic infection before and after the PAD...
January 11, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29330994/pattern-of-causative-micro-organisms-in-catheter-related-blood-stream-infections-in-dialysis-patients-experience-from-saudi-arabia
#7
Bilal Mohsin
BACKGROUND: Catheter related blood stream infections (CRBSI) are the leading cause of morbidity in HD patients. The majority of these infections relate to haemodialysis catheters. There is a paucity of local data on microbial agents responsible for CRBSI in our region. This prompted our study. METHODS: This Prospective observatory survey was conducted in Department of Nephrology, King Fahd Hospital, Hofuf KSA from Nov 2014 to Jan 2017 (26 months). It was performed on dialysis patients with HD catheters who developed features of CRBSI...
October 2017: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/29327470/investigation-of-the-effect-of-rapid-and-slow-external-ph-increases-on-enterococcus-faecalis-biofilm-grown-on-dentine
#8
Mark Stenhouse, Peter Zilm, Jithendra Ratnayake, Peter Cathro
BACKGROUND: Calcium hydroxide is a common endodontic medicament and has an anti-microbial effect by increasing the localized pH within the root canal. However, Enterococcus faecalis has shown some resistance to calcium hydroxide. METHOD: A flow cell apparatus was used to grow an E. faecalis biofilm on dentine discs. Following 4 weeks growth in Todd Hewitt Broth, flow cells were exposed to either a rapid or slow increase to pH 11.5 or 12.5. Cellular viability was determined using serial plating and the number of colony forming units was normalised against the cellular protein content...
January 11, 2018: Australian Dental Journal
https://www.readbyqxmd.com/read/29326225/genomic-insights-into-biofilm-forming-enterococcus-faecalis-sk460-isolated-from-a-chronic-diabetic-ulcer-patient
#9
Karthika Suryaletha, Lekshmi Narendrakumar, Joby John, Dinesh Reghunathan, Manoj Prasannakumar, Sabu Thomas
Enterococcus faecalis is recognized as one of the leading pathogens causing nosocomial infections. Here we report a draft genome sequence of Enterococcus faecalis SK460, isolated from a chronic diabetic foot ulcer patient. This strain exhibits various biofilm-associated genes, virulence genes, and antibiotic-resistance genes related to aminoglycoside, macrolide, and tetracycline resistance.
January 11, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29325872/structure-and-function-of-a-highly-active-bile-salt-hydrolase-bsh-from-enterococcus-faecalis-and-post-translational-processing-of-bsh-enzymes
#10
Deepak Chand, Priyabrata Panigrahi, Nishant Kumar Varshney, Sureshkumar Ramasamy, C G Suresh
Bile Salt Hydrolase (BSH), a member of Cholylglycine hydrolase family, catalyses the de-conjugation of bile acids and is evolutionarily related to penicillin V acylase (PVA) that hydrolyses a different substrate such as penicillin V. We report the three-dimensional structure of a BSH enzyme from the Gram-positive bacteria Enterococcus faecalis (EfBSH) which has manifold higher hydrolase activity compared to other known BSHs and displays unique allosteric catalytic property. The structural analysis revealed reduced secondary structure content compared to other known BSH structures, particularly devoid of an anti-parallel β-sheet in the assembly loop and part of a β-strand is converted to increase the length of a substrate binding loop 2...
January 8, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29325735/whole-transcriptome-analysis-reveals-potential-novel-mechanisms-of-low-level-linezolid-resistance-in-enterococcus-faecalis
#11
Ruoyi Hua, Yun Xia, Wenyao Wu, Jia Yan, Mi Yang
Linezolid is an oxazolidinone antibiotic commonly used to treat serious infections caused by vancomycin-resistant enterococcus. Recently, low-level linezolid resistant Enterococcus faecalis strains have emerged worldwide, but the resistant mechanisms remain undefined. Whole-transcriptome profiling was performed on an E. faecalis strain P10748 with low-level linezolid resistance in comparison with a linezolid-susceptible strain 3138 and the standard control strain ATCC29212. The functions of differentially expressed genes (DEGs) were predicted, with some DEGs potentially involved in drug resistance were validated by PCR and quantitative PCR (qPCR)...
January 8, 2018: Gene
https://www.readbyqxmd.com/read/29324657/%C3%AE-glucosidase-inhibition-and-antibacterial-activity-of-secondary-metabolites-from-the-ecuadorian-species-clinopodium-taxifolium-kunth-govaerts
#12
Vladimir Morocho, Andrea Valle, Jessica García, Gianluca Gilardoni, Luis Cartuche, Alírica I Suárez
The phytochemical investigation of both volatile and fixed metabolites of Clinopodium taxifolium (Kunth) Govaerts (Lamiaceae) was performed for the first time. It allowed the isolation and characterization of the essential oil and six known compounds: carvacrol (1), squalane (2), uvaol (3), erythrodiol (4), ursolic acid (5), and salvigenin (6). Their structures were identified and characterized by Nuclear Magnetic Resonance (NMR) and Gas Chromatography coupled to Mass Spectroscopy (GC-MS), and corroborated by literature...
January 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29321302/rapid-seasonal-evolution-in-innate-immunity-of-wild-drosophila-melanogaster
#13
Emily L Behrman, Virginia M Howick, Martin Kapun, Fabian Staubach, Alan O Bergland, Dmitri A Petrov, Brian P Lazzaro, Paul S Schmidt
Understanding the rate of evolutionary change and the genetic architecture that facilitates rapid adaptation is a current challenge in evolutionary biology. Comparative studies show that genes with immune function are among the most rapidly evolving genes across a range of taxa. Here, we use immune defence in natural populations of Drosophila melanogaster to understand the rate of evolution in natural populations and the genetics underlying rapid change. We probed the immune system using the natural pathogens Enterococcus faecalis and Providencia rettgeri to measure post-infection survival and bacterial load of wild D...
January 10, 2018: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/29319752/effectiveness-of-aqueous-and-hydroalcoholic-extracts-of-acanthospermum-australe-loefl-kuntze-against-diarrhea-inducing-bacteria
#14
R Mallmann, E M Ethur, P Bianchetti, D Faleiro, L Hoehne, M I Goettert
Leaves and roots of Acanthospermum australe (Asteraceae) have been used in Brazilian folk medicine for the treatment of various ailments including diarrhea, skin diseases, blennorrhagia, dyspepsia, parasitic worms and malaria. The aim of study was to characterize the chemical profiles of the aqueous and hydroalcoholic extracts of leaves and roots of A. australe, and to evaluate their antimicrobial activities against diarrhea-inducing bacteria (Enterococcus faecalis, Shigella dysenteriae and Yersinia enterocolitica), as well as their cytotoxic properties...
January 8, 2018: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/29319507/inhibition-of-multidrug-resistant-gram-positive-and-gram-negative-bacteria-by-a-photoactivated-porphyrin
#15
Moreno Bondi, Anna Mazzini, Simona de Niederhäusern, Ramona Iseppi, Patrizia Messi
The authors studied the in vitro antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecyl-pyridyl)porphine (C14) against four multidrug-resistant bacteria: Staphylococcus aureus, Enterococcus faecalis (Gram-positive), Escherichia coli, Pseudomonas aeruginosa (Gram-negative). Using 10 μg/ml of porphyrin and 60 sec irradiation we observed the remarkable susceptibility of S. aureus and E. faecalis to treatment while, under the same conditions, E. coli and P. aeruginosa showed very low susceptibility...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319420/molecular-identification-and-phylogenetic-analysis-of-bothrops-insularis-bacterial-and-fungal-microbiota
#16
Lidiane Nunes Barbosa, Rui Seabra Ferreira, Priscila Luiza Mello, Hans Garcia Garces, Jéssica Luana Chechi, Tarsila Frachin, Luciana Curtolo De Barros, Sandra De Moraes Guimenes Bosco, Eduardo Bagagli, Ary Fernandes Júnior, Benedito Barraviera, Lucilene Delazari Dos Santos
Bothrops insularis, known as the golden lancehead snake, has its natural habitat restricted to Queimada Grande Island on the southern coast of Brazil. This culture-dependent study aimed to identify microorganisms obtained from the mouth, eyes, and cloaca of this species. Swabs from 20 snakes were collected for fungal and bacterial isolation. DNA was extracted from all samples, and identification was performed by amplifying the ITS1-5.8S-ITS2 regions and the 16S rDNA gene, respectively. All strains were identified and deposited in the GenBank nucleotide database...
January 10, 2018: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/29319345/surface-properties-of-enterococcus-faecalis-cells-isolated-from-chicken-hearts-determine-their-low-ability-to-form-biofilms
#17
Jolanta Cieśla, Dagmara Stępień-Pyśniak, Agnieszka Nawrocka, Małgorzata Łukowska, Tomasz Hauschild, Andrzej Wernicki, Andrzej Bieganowski
Enterococcus faecalis is one of the most significant bacterial pathogens associated with the first-week mortality of chickens. Here, the surface properties of bacterial cells and the selected virulence factors of E. faecalis strains isolated from the hearts of clinically healthy broiler chickens were studied. Investigations were carried out on live and autoclaved cells. E. faecalis (ATCC 29212) was used as a reference strain. The bacterial cells revealed different haemolytic activities. Their surface free energy was dominated by the hydrophobic component...
January 10, 2018: Biofouling
https://www.readbyqxmd.com/read/29318387/antimicrobial-activity-of-psidium-cattleianum-associated-with-calcium-hydroxide-against-enterococcus-faecalis-and-candida-albicans-an-in-vitro-study
#18
Jorgiana Sangalli, Elerson Gaetti Jardim Júnior, Carlos Roberto Emerenciano Bueno, Rogério Castilho Jacinto, Gustavo Sivieri-Araújo, João Eduardo Gomes Filho, Luciano Tavares Ângelo Cintra, Eloi Dezan Junior
OBJECTIVE: Evaluate, in vitro, the antimicrobial activity of Psidium cattleianum leaf extracts combined with calcium hydroxide against Enterococcus faecalis and Candida albicans biofilm. MATERIALS AND METHODS: Dentin specimens obtained from extracted bovine incisors were infected during 14 days with E. faecalis ATCC 29212 and C. albicans ATCC 10231. The specimens were filled with calcium hydroxide pastes prepared with the following vehicles: Psidium cattleianum ethanolic, Psidium cattleianum propylene glycolic, distilled water, and saline as control...
January 9, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29317913/prevalence-and-risk-factors-for-bloodstream-infection-present-on-hospital-admission
#19
Sainfer Aliyu, Bevin Cohen, Jianfang Liu, Elaine Larson
Background: Bloodstream infection present on hospital admission (BSI-POA) is a major cause of morbidity and mortality. The purpose of this study was to measure prevalence and describe the risk factors of patients with BSI-POA and to determine the prevalence of resistance in isolates by admission source. Methods: We conducted a retrospective cohort study of patients discharged from three hospitals in New York City between 2006 and 2014. BSI-POA was defined as BSI diagnosed within 48 h of hospitalisation...
January 2018: Journal of Infection Prevention
https://www.readbyqxmd.com/read/29317817/antibacterial-activity-of-trimetal-cuznfe-oxide-nanoparticles
#20
Khalid E Alzahrani, Abdurahman A Niazy, Abdullah M Alswieleh, Rizwan Wahab, Ahmed M El-Toni, Hamdan S Alghamdi
Background: The increasing resistance of pathogenic bacteria to antibiotics is a challenging worldwide health problem that has led to the search for new and more efficient antibacterial agents. Nanotechnology has proven to be an effective tool for the fight against bacteria. Methods: In this paper, we present the synthesis and traits of trimetal (CuZnFe) oxide nanoparticles (NPs) using X-ray diffraction, high-resolution transmission electron microscopy, and energy dispersive x-ray spectroscopy...
2018: International Journal of Nanomedicine
keyword
keyword
29440
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"