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Enterococcus faecalis

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https://www.readbyqxmd.com/read/28441606/growth-inhibition-and-antibiofilm-potential-of-ag-nanoparticles-coated-with-lectin-an-arthropod-immune-molecule
#1
Sangily Jayanthi, Sathappan Shanthi, Baskaralingam Vaseeharan, Narayanan Gopi, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Giovanni Benelli
Lectins from the haemolymph of arthropods, including crustaceans, are molecules potentially involved in the immune recognition and phagocytosis. Here, lectin was purified from the haemolymph of blue swimmer crab Portunus pelagicus, using mannose-coupled sepharose CL-4B affinity column chromatography. In SDS-PAGE analysis, lectin showed a molecular mass of approximately 155kDa. The synthesis of lectin-coated silver nanoparticles (lectin-coated AgNPs) was confirmed by UV-Vis spectroscopy, XRD, FTIR, SEM, TEM, SAED, and EDX analysis...
April 13, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28439299/substance-p-enhances-lactic-acid-and-tyramine-production-in-enterococcus-faecalis-v583-and-promotes-its-cytotoxic-effect-on-intestinal-caco-2-tc7-cells
#2
Kelly Biaggini, Valérie Borrel, Sabine Szunerits, Rabah Boukherroub, Awa N'Diaye, Arthur Zébré, Maryse Bonnin-Jusserand, Guillaume Duflos, Marc Feuilloley, Djamel Drider, Pierre Déchelotte, Nathalie Connil
BACKGROUND: Enterococcus faecalis, generally considered as a saprophytic bowel commensal, has recently emerged as an important nosocomial pathogen causing severe urinary tract infections, surgical wound infections, bacteremia, and bacterial endocarditis. This bacterium is capable of forming biofilms on various surfaces and its high level of antibiotic resistance contributes to its pathogenicity. The aim of this study was to evaluate the effect on E. faecalis, of Substance P (SP), an antimicrobial peptide that is produced in the gut and skin...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28438824/comparison-of-linezolid-and-vancomycin-lock-solutions-with-and-without-heparin-against-biofilm-producing-bacteria
#3
Megan K Luther, Leonard A Mermel, Kerry L LaPlante
PURPOSE: The activity of linezolid and vancomycin lock solutions against biofilm-producing strains of Staphylococcus aureus, S. epidermidis, and Enterococcus faecalis was studied. METHODS: Two strains each of methicillin-susceptible S. aureus (MSSA), methicillin-resistant S. aureus (MRSA), and S. epidermidis, and 1 strain of vancomycin-susceptible E. faecalis and vancomycin-resistant E. faecalis were tested against vancomycin and linezolid to assess prevention of biofilm formation and eradication of these pathogens within a formed biofilm...
May 1, 2017: American Journal of Health-system Pharmacy: AJHP
https://www.readbyqxmd.com/read/28435376/comparative-evaluation-of-antimicrobial-efficacy-of-an-alternative-natural-agent-for-disinfection-of-toothbrushes
#4
R Vignesh, C Vishnu Rekha, Parisa Norouzi Baghkomeh, Sankar Annamalai, Ditto Sharmin
OBJECTIVE: The aim of this study was to assess the antimicrobial potency of aqueous extract of Psidium guajava leaves in two different concentrations as a toothbrush disinfectant against three oral bacterial species. MATERIALS AND METHODS: Aqueous extracts of P. guajava leaves were prepared at 20% and 30% concentrations and 0.2% chlorhexidine was used as control. The toothbrushes were equally divided into 9 groups with 10 toothbrushes per disinfectant, which were contaminated with Streptococcus mutans, Lactobacillus acidophilus, and Enterococcus faecalis...
January 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/28435366/comparative-evaluation-of-antimicrobial-efficacy-of-triple-antibiotic-paste-calcium-hydroxide-and-a-proton-pump-inhibitor-against-resistant-root-canal-pathogens
#5
Shibha Mehta, Promila Verma, Aseem Prakash Tikku, Anil Chandra, Rhythm Bains, Gopa Banerjee
OBJECTIVE: The objective of this study is to compare the antimicrobial efficacy of triple antibiotic paste (TAP) and a proton pump inhibitor (PPI) (omeprazole) in combination with calcium hydroxide (CH) against Enterococcus faecalis and Candida albicans. MATERIALS AND METHODS: E. faecalis and C. albicans were subcultured and inoculated at 37° overnight and were treated with different dilutions of TAP, 25 μg/ml (Group 1), CH (Group 2, control), CH 16 mg/ml + omeprazole 2 mg/ml (Group 3a) (CH 16 mg/ml + omeprazole 4 mg)/ml (Group 3b) for 24, 48, and 72 h in sterile uncoated 96-well microtiter plates...
January 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/28435359/comparative-antimicrobial-efficacy-of-selected-root-canal-irrigants-on-commonly-isolated-microorganisms-in-endodontic-infection
#6
Sandeep Dubey, Suparna Ganguly Saha, Balakrishnan Rajkumar, Tapan Kumar Dhole
OBJECTIVE: This study aims to evaluate and compare the antimicrobial efficacy of three selected root canal irrigants (BioPure MTAD, metronidazole, aztreonam) against microbes commonly isolated from polymicrobial microbiota of root canal infection. MATERIALS AND METHODS: This study was designed with four experimental groups (Group I - Bacteroides fragilis, Group II - Propionibacterium acnes, Group III - Enterococcus faecalis, Group IV - Candida albicans) based on the microbes selected for the study...
January 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/28434876/dual-targeting-small-molecule-inhibitors-of-the-staphylococcus-aureus-fmn-riboswitch-disrupt-riboflavin-homeostasis-in-an-infectious-setting
#7
Hao Wang, Paul A Mann, Li Xiao, Charles Gill, Andrew M Galgoci, John A Howe, Artjohn Villafania, Christopher M Barbieri, Juliana C Malinverni, Xinwei Sher, Todd Mayhood, Megan D McCurry, Nicholas Murgolo, Amy Flattery, Matthias Mack, Terry Roemer
Riboswitches are bacterial-specific, broadly conserved, non-coding RNA structural elements that control gene expression of numerous metabolic pathways and transport functions essential for cell growth. As such, riboswitch inhibitors represent a new class of potential antibacterial agents. Recently, we identified ribocil-C, a highly selective inhibitor of the flavin mononucleotide (FMN) riboswitch that controls expression of de novo riboflavin (RF, vitamin B2) biosynthesis in Escherichia coli. Here, we provide a mechanistic characterization of the antibacterial effects of ribocil-C as well as of roseoflavin (RoF), an antimetabolite analog of RF, among medically significant Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) and Enterococcus faecalis...
April 18, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28433695/disinfection-of-water-inoculated-with-enterococcus-faecalis-using-solar-fe-iii-edds-h2o2-or-s2o8-2-process
#8
A Bianco, M I Polo López, P Fernández Ibáñez, M Brigante, G Mailhot
In this study, the activation of H2O2 and persulfate ions induced by solar photolysis of Fe(III)EDDS complex were investigated in water disinfection, applying solar AOPs processes. The use of Fe(III)EDDS complex maintains iron in soluble form until slightly basic pH and so the photolysis is efficient in a large range of pH compatible with natural waters. Moreover, for the first time, the impact of photogenerated hydroxyl and sulfate radicals on the inactivation of Enterococcus faecalis in water was studied...
April 18, 2017: Water Research
https://www.readbyqxmd.com/read/28432093/diversity-of-integrative-and-conjugative-elements-of-streptococcus-salivarius-and-their-intra-and-interspecies-transfer
#9
Narimane Dahmane, Virginie Libante, Florence Charron-Bourgoin, Eric Guédon, Gérard Guédon, Nathalie Leblond-Bourget, Sophie Payot
Integrative and Conjugative Elements (ICEs) are widespread chromosomal mobile genetic elements, which can transfer autonomously by conjugation in bacteria. Thirteen ICEs with a conjugation module closely related to that of ICESt3 of Streptococcus thermophilus were characterized in Streptococcus salivarius by whole genome sequencing. Sequence comparison highlighted ICE evolution by shuffling of 3 different integration/excision modules (for integration in the 3' end of fda, rpsI or rpmG genes) with the conjugation module of the ICESt3 subfamily...
April 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28431851/ultraviolet-c-light-as-a-means-of-disinfecting-anesthesia-workstations
#10
Matthew Nottingham, Gene Peterson, Christopher Doern, Michelle Doll, Nadia Masroor, Kakotan Sanogo, Michael Stevens, Gonzalo Bearman
BACKGROUND: Anesthesia workstations (AWs) are a reservoir for pathogenic organisms potentially associated with surgical site infections. This study examined the effectiveness of the Tru-D SmartUVC device (Tru-D LLC, Nashville, TN) on bioburden reduction (BR) on AWs. METHODS: Strips of tissue inoculated with a known concentration of either Staphylococcus aureus, Enterococcus faecalis, or Acinetobacter sp were placed on 22 high-touch surfaces of an AW. Half of the AW surfaces received direct ultraviolet (UV) light exposure and half received indirect exposure...
April 18, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28427133/-effects-of-application-of-vancomycin-in-the-early-stage-of-patients-with-extremely-severe-burn
#11
Z Zhu, G W Cao, J J Bao, Z H Hu, Z Shen, H Tao, B Cao, F Xu
Objective: To evaluate the effects of application of vancomycin in the early stage of patients with extremely severe burn, in order to provide reference to drug for anti-infection treatment in the early stage of patients with extremely severe burn. Methods: Data of 15 patients of Kunshan explosion on August 2nd, 2014, admitted to the Department of Intensive Care in our hospital were retrospectively analyzed. The clinical efficacy of continuously intravenous dripping of vancomycin (combined with imipenem) in the early stage of burns (before and on post burn day 14) was analyzed...
April 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28426772/correction-chitosan-propolis-nanoparticle-formulation-demonstrates-anti-bacterial-activity-against-enterococcus-faecalis-biofilms
#12
Teik Hwa Ong, Ebenezer Chitra, Srinivasan Ramamurthy, Rajinikanth Paruvathanahalli Siddalingam, Kah Hay Yuen, Stephen Periathamby Ambu, Fabian Davamani
[This corrects the article DOI: 10.1371/journal.pone.0174888.].
2017: PloS One
https://www.readbyqxmd.com/read/28426292/efficacy-of-three-different-lasers-on-eradication-of-enterococcus-faecalis-and-candida-albicans-biofilms-in-root-canal-system
#13
Silvija Kasić, Marita Knezović, Nataša Beader, Dragana Gabrić, Ana Ivanišević Malčić, Anja Baraba
OBJECTIVE: The objective was to compare the efficacy of three different lasers in disinfection of root canals inoculated with Enterococcus faecalis and Candida albicans biofilms. BACKGROUND: Endodontic space disinfection depends on the type of root canal irrigant used and the way it is delivered and agitated because irrigants have limited ability to reach all parts of root canal system. MATERIALS AND METHODS: Thirty single-rooted human teeth were selected...
April 20, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28424670/azathioprine-mercaptopurine-and-5-aminosalicylic-acid-affect-the-growth-of-ibd-associated-campylobacter-species-and-other-enteric-microbes
#14
Fang Liu, Rena Ma, Stephen M Riordan, Michael C Grimm, Lu Liu, Yiming Wang, Li Zhang
Campylobacter concisus is a bacterium that is associated with inflammatory bowel disease (IBD). Immunosuppressive drugs including azathioprine (AZA) and mercaptopurine (MP), and anti-inflammatory drug such as 5-aminosalicylic acid (5-ASA) are commonly used to treat patients with IBD. This study aimed to examine the effects of AZA, MP, and 5-ASA on the growth of IBD-associated bacterial species and to identify bacterial enzymes involved in immunosuppressive drug metabolism. A total of 15 bacterial strains of five species including 11 C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28424663/antimicrobial-photodynamic-therapy-against-endodontic-enterococcus-faecalis-and-candida-albicans-mono-and-mixed-biofilms-in-the-presence-of-photosensitizers-a-comparative-study-with-classical-endodontic-irrigants
#15
Patrícia Diogo, Chantal Fernandes, Francisco Caramelo, Marta Mota, Isabel M Miranda, M A F Faustino, M G P M S Neves, Marciana P Uliana, Kleber T de Oliveira, João M Santos, Teresa Gonçalves
Endodontic biofilms eradication from the infected root canal system remains as the primary focus in endodontic field. In this study, it was assessed the efficacy of antimicrobial Photodynamic Therapy (aPDT) with the Zn(II)chlorin e6 methyl ester (Zn(II)e6Me) activated by red light against monospecies and mixed biofilms of Enterococcus faecalis and Candida albicans. The results were compared with the ones obtained with Rose Bengal (RB), Toluidine Blue-O (TBO), the synthetic tetracationic porphyrin (TMPyP) as well as classical endodontic irrigants (3% NaOCl, 17% EDTA and 2% CHX)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28423018/comprehensive-analysis-of-phospholipids-and-glycolipids-in-the-opportunistic-pathogen-enterococcus-faecalis
#16
Rafi Rashid, Amaury Cazenave-Gassiot, Iris H Gao, Zeus J Nair, Jaspal K Kumar, Liang Gao, Kimberly A Kline, Markus R Wenk
Enterococcus faecalis is a Gram-positive, opportunistic, pathogenic bacterium that causes a significant number of antibiotic-resistant infections in hospitalized patients. The development of antibiotic resistance in hospital-associated pathogens is a formidable public health threat. In E. faecalis and other Gram-positive pathogens, correlations exist between lipid composition and antibiotic resistance. Resistance to the last-resort antibiotic daptomycin is accompanied by a decrease in phosphatidylglycerol (PG) levels, whereas multiple peptide resistance factor (MprF) converts anionic PG into cationic lysyl-PG via a trans-esterification reaction, providing resistance to cationic antimicrobial peptides...
2017: PloS One
https://www.readbyqxmd.com/read/28420830/antimicrobial-and-antibiofilm-activities-of-mta-supplemented-with-bismuth-lipophilic-nanoparticles
#17
Rene Hernandez-Delgadillo, Casiano Del Angel-Mosqueda, Juan Manuel Solís-Soto, Silvia Munguia-Moreno, Nayely Pineda-Aguilar, Rosa Isela Sánchez-Nájera, Shankararaman Chellam, Claudio Cabral-Romero
The objective of this work was to determine the antimicrobial and antibiofilm properties of mineral trioxide aggregate (MTA) supplemented with bismuth lipophilic nanoparticles (BisBAL NPs). The antimicrobial activity of the composite MTA-BisBAL NPs was determined by the disk diffusion assay, while antibiofilm activity was analyzed by fluorescence microscopy. The cytotoxicity of MTA-BisBAL NPs was determined on human gingival fibroblasts by optical microscopy and crystal violet staining. MTA-BisBAL NPs inhibited the growth of Enterococcus faecalis, Escherichia coli, and Candida albicans and also detached the biofilm of fluorescent E...
April 18, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/28417533/split-tasks-of-asymmetric-nucleotide-binding-sites-in-the-heterodimeric-abc-exporter-efrcd
#18
Lea M Hürlimann, Michael Hohl, Markus A Seeger
Many heterodimeric ATP-binding cassette (ABC) exporters evolved asymmetric ATP-binding sites containing a degenerate site incapable of ATP hydrolysis due to noncanonical substitutions in conserved sequence motifs. Recent studies revealed that nucleotide binding to the degenerate site stabilizes contacts between the nucleotide-binding domains (NBDs) of the inward-facing transporter and regulates ATP hydrolysis at the consensus site via allosteric coupling mediated by the D-loops. However, it is unclear whether nucleotide binding to the degenerate site is strictly required for substrate transport...
April 18, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28416303/effect-of-instrumentation-techniques-and-preparation-taper-on-apical-extrusion-of-bacteria
#19
Hacer Aksel, Selen Küçükkaya Eren, Aslı Çakar, Ahmet Serper, Cumhur Özkuyumcu, Adham A Azim
INTRODUCTION: The aim of this in vitro study was to evaluate the effects of different root canal instrumentation techniques and preparation tapers on the amount of apically extruded bacteria. METHODS: The root canals of 98 extracted human mandibular incisors were contaminated with Enterococcus faecalis suspension. After incubation at 37°C for 24 hours, the root canals were instrumented with K3 rotary files in a crown-down (CD) or full-length linear instrumentation technique (FL) by using 3 different root canal tapers (0...
April 14, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28416302/effect-of-bacterial-biofilm-on-the-osteogenic-differentiation-of-stem-cells-of-apical-papilla
#20
Lakshmi Vishwanat, Rose Duong, Koyo Takimoto, Linda Phillips, Claudia O Espitia, Anibal Diogenes, Shivani B Ruparel, David Kolodrubetz, Nikita B Ruparel
INTRODUCTION: Although clinical success in regenerative endodontics is substantially high, histological success is limited to finding bone/cementum-like tissue instead of dentin within the canal space. The aims of this study were to investigate (1) the effect of bacterial biofilm on osteogenic gene expression in stem cells of the apical papilla (SCAP) and (2) the effect of bacterial antigens on the functional differentiation of SCAP into a mineralizing phenotype. METHODS: Using an ex vivo organotypic root canal model and an American Association of Endontists-recommended regenerative endodontic procedures, we evaluated SCAP differentiation in the presence and absence of an Enterococcus faecalis biofilm...
April 14, 2017: Journal of Endodontics
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