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Enterococcus faecalis

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https://www.readbyqxmd.com/read/28221886/technological-properties-and-biogenic-amines-production-by-bacteriocinogenic-lactococci-and-enterococci-strains-isolated-from-raw-goat-s-milk
#1
Luana Martins Perin, Simona Belviso, Barbara Dal Bello, Luís Augusto Nero, Luca Cocolin
Technological properties and biogenic amine production were analyzed in 56 bacteriocinogenic lactococci and enterococci strains isolated from raw goat's milk. Fifteen lactococci strains were able to reduce milk pH to 5.3 or lower after 6 h, while enterococci strains were initially slow in producing acids. Lactococcus lactis subsp. lactis GLc06 and three strains of Enterococcus faecalis (GEn20, GEn22, and GEn23) presented high proteolytic activity. L. lactis subsp. lactis GLc06 and E. faecalis GEn22 also showed a high percentage of autolysis after only 4 h, reaching 71...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28219792/conjugative-type-iv-secretion-in-gram-positive-pathogens-trag-a-lytic-transglycosylase-and-endopeptidase-interacts-with-translocation-channel-protein-tram
#2
Verena Kohler, Ines Probst, Andreas Aufschnaiter, Sabrina Büttner, Lisa Schaden, Gerald Rechberger, Günther Koraimann, Elisabeth Grohmann, Walter Keller
Conjugative transfer plays a major role in the transmission of antibiotic resistance in bacteria. pIP501 is a Gram-positive conjugative model plasmid with the broadest transfer host-range known so far and is frequently found in Enterococcus faecalis and Enterococcus faecium clinical isolates. The pIP501 type IV secretion system is encoded by 15 transfer genes. In this work, we focus on the VirB1-like protein TraG, a modular peptidoglycan metabolizing enzyme, and the VirB8-homolog TraM, a potential member of the translocation channel...
February 17, 2017: Plasmid
https://www.readbyqxmd.com/read/28209400/multidrug-resistant-enterococcal-infections-new-compounds-novel-antimicrobial-therapies
#3
REVIEW
Roel M van Harten, Rob J L Willems, Nathaniel I Martin, Antoni P A Hendrickx
Over the past two decades infections due to antibiotic-resistant bacteria have escalated world-wide, affecting patient morbidity, mortality, and health care costs. Among these bacteria, Enterococcus faecium and Enterococcus faecalis represent opportunistic nosocomial pathogens that cause difficult-to-treat infections because of intrinsic and acquired resistance to a plethora of antibiotics. In recent years, a number of novel antimicrobial compound classes have been discovered and developed that target Gram-positive bacteria, including E...
February 13, 2017: Trends in Microbiology
https://www.readbyqxmd.com/read/28204495/early-in-vitro-development-of-daptomycin-non-susceptibility-in-high-level-aminoglycoside-resistant-enterococcus-faecalis-predicts-the-efficacy-of-the-combination-of-high-dose-daptomycin-plus-ampicillin-in-an-in-vivo-model-of-experimental-endocarditis
#4
J M Pericàs, C García-de-la-Mària, M Brunet, Y Armero, J García-González, G Casals, M Almela, E Quintana, C Falces, S Ninot, D Fuster, J Llopis, F Marco, A Moreno, J M Miró
No abstract text is available yet for this article.
February 14, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28203232/cationic-net-charge-and-counter-ion-type-as-antimicrobial-activity-determinant-factors-of-short-lipopeptides
#5
Katarzyna E Greber, Malgorzata Dawgul, Wojciech Kamysz, Wieslaw Sawicki
To get a better insight into the antimicrobial potency of short cationic lipopeptides, 35 new entities were synthesized using solid phase peptide strategy. All newly obtained lipopeptides were designed to be positively charged from +1 to +4. This was achieved by introducing basic amino acid - lysine - into the lipopeptide structure and had a hydrophobic fatty acid chain attached. Lipopeptides were subjected to microbiological tests using reference strains of Gram-negative bacteria: Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Gram-positive bacteria: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Enterococcus faecalis, and fungi: Candida albicans, Candida tropicalis, Aspergillus brasiliensis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28203227/some-technological-properties-of-lactic-acid-bacteria-isolated-from-dahi-and-datshi-naturally-fermented-milk-products-of-bhutan
#6
H N J Shangpliang, Sharmila Sharma, Ranjita Rai, Jyoti P Tamang
Dahi and datshi are common naturally fermented milk (NFM) products of Bhutan. Population of lactic acid bacteria (LAB) in dahi (pH 3.7) and datshi (pH 5.2) was 1.4 × 10(7) and 3.9 × 10(8) cfu/ml, respectively. Based on 16S rRNA gene sequencing isolates of LAB from dahi and datshi were identified as Enterococcus faecalis, E. faecium, Lactococcus lactis subsp. lactis. LAB strains were tested for some technological properties. All LAB strains except E. faecalis CH2:17 caused coagulation of milk at both 30°C for 48 h...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28202007/diffusible-substances-from-lactic-acid-bacterial-cultures-exert-strong-inhibitory-effects-on-listeria-monocytogenes-and-salmonella-enterica-serovar-enteritidis-in-a-co-culture-model
#7
Solomon H Mariam, Nigus Zegeye, Abraham Aseffa, Rawleigh Howe
BACKGROUND: Food-borne infections cause huge economic and human life losses. Listeria monocytogenes and Salmonella enterica serovar Enteritidis are among the top ranking pathogens causing such losses. Control of such infections is hampered by persistent contamination of foods and food-processing environments, resistance of pathogens to sanitizing agents, existence of heterogeneous populations of pathogens (including culturable and viable but non-culturable cells) within the same food items, and inability to detect all such pathogens by culture-based methods...
February 15, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28201936/effects-of-lactobacillus-casei-and-enterococcus-faecalis-on-growth-performance-immune-function-and-gut-microbiota-of-suckling-piglets
#8
Chaoqi Liu, Qun Zhu, Juan Chang, Qingqiang Yin, Andong Song, Zhentian Li, Erzhu Wang, Fushan Lu
This study was carried out to investigate the effects of orally administrated Lactobacillus casei and Enterococcus faecalis on performance, immune function and gut microbiota of suckling piglets. Neonatal piglets (n = 120) were randomly assigned to 4 groups, with 30 suckling piglets in each group. The piglets were from 15 litters, one male and one female piglet were selected for each group in each litter. The Control group was administrated with normal saline, the other groups with L. casei or E. faecalis or a combination of L...
April 2017: Archives of Animal Nutrition
https://www.readbyqxmd.com/read/28197728/linezolid-resistant-enterococci-in-polish-hospitals-species-clonality-and-determinants-of-linezolid-resistance
#9
I Gawryszewska, D Żabicka, W Hryniewicz, E Sadowy
The significant increase of the linezolid-resistant enterococci (LRE) has been observed in Polish hospitals since 2012 and our study aimed at elucidating the possible reasons for this phenomenon. Polish LRE isolates were analysed by multilocus-sequence typing (MLST) and multiple locus variable-number tandem repeat (VNTR) analysis (MLVA), polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (PCR-RFLP) to establish clonal relatedness and mechanism of linezolid resistance, respectively...
February 14, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28194365/antibacterial-effect-of-urushiol-on-e-faecalis-as-a-root-canal-irrigant
#10
Sang-Wan Kim, Dong-Hoon Shin
OBJECTIVES: The purpose of this study was to compare the antibacterial activity of urushiol against Enterococcus faecalis (E. faecalis) to that of NaOCl. MATERIALS AND METHODS: The canals of thirty two single rooted human teeth were instrumented with Ni-Ti files (ProTaper Next X1, X2, X3, Dentsply). A pure culture of E. faecalis ATCC 19433 was prepared in sterile brain heart infusion (BHI) broth. The teeth were submerged in the suspension of E. faecalis and were incubated at 37℃ for 7 days to allow biofilm formation...
February 2017: Restorative Dentistry & Endodontics
https://www.readbyqxmd.com/read/28193569/analysis-of-the-expression-of-nlrp3-and-aim2-in-periapical-lesions-with-apical-periodontitis-and-microbial-analysis-outside-the-apical-segment-of-teeth
#11
Shujun Ran, Bin Liu, Shensheng Gu, Zhe Sun, Jingping Liang
OBJECTIVE: To detect the distribution and expression levels of the nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) and the absent in Melanoma 2 (AIM2) inflammasomes in periapical lesions and to analyse the possible microbial stimuli outside of teeth. DESIGN: The distribution of NLRP3 and AIM2 inflammasomes in sixteen periapical lesions was investigated by immunohistochemistry. Meanwhile, the relative gene expression levels of NLRP3 and AIM2 in sixteen periapical lesions and three health periodontal tissue were quantified by real-time polymerase chain reaction (PCR)...
February 6, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28186464/evaluation-of-antimicrobial-photodynamic-therapy-using-indocyanine-green-and-near-infrared-diode-laser-against-enterococcus-faecalis-in-infected-human-root-canals
#12
Charis Beltes, Nikolaos Economides, Hercules Sakkas, Chrissanthy Papadopoulou, Theodoros Lambrianidis
OBJECTIVE: Photodynamic therapy (PDT) is considered a promising adjunct to the currently available endodontic disinfection techniques leading to more effective reduction of intracanal bacteria. The present ex vivo study aimed to assess the antimicrobial effect of PDT using indocyanine green (ICG) as photosensitizer and a near-infrared (NIR) diode laser in root canals of human teeth infected with Enterococcus faecalis. MATERIALS AND METHODS: Ninety single-rooted teeth after chemomechanical preparation and sterilization were contaminated with an E...
February 9, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28185866/combined-chemical-and-physical-transformation-method-with-rbcl-and-sepiolite-for-the-transformation-of-various-bacterial-species
#13
Jun Ren, Haram Lee, Seung Min Yoo, Myeong-Sang Yu, Hansoo Park, Dokyun Na
DNA transformation that delivers plasmid DNAs into bacterial cells is fundamental in genetic manipulation to engineer and study bacteria. Developed transformation methods to date are optimized to specific bacterial species for high efficiency. Thus, there is always a demand for simple and species-independent transformation methods. We herein describe the development of a chemico-physical transformation method that combines a rubidium chloride (RbCl)-based chemical method and sepiolite-based physical method, and report its use for the simple and efficient delivery of DNA into various bacterial species...
February 7, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28185089/effectiveness-of-repeated-photodynamic-therapy-in-the-elimination-of-intracanal-enterococcus-faecalis-biofilm-an-in-vitro-study
#14
Ewa Joanna Prażmo, Renata Alicja Godlewska, Agnieszka Beata Mielczarek
The study aimed to investigate the effectiveness of photodynamic therapy in the elimination of intracanal Enterococcus faecalis biofilm and to analyse how a repeated light irradiation, replenishment of oxygen and photosensitiser affect the results of the photodynamic disinfecting protocol. After chemomechanical preparation, 46 single-rooted human teeth were infected with a clinical strain of E. faecalis and incubated for a week in microaerobic conditions. The experimental procedures included groups of single application of photodynamic therapy, two cycles of PDT, irrigation with 5...
February 10, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28184339/correlation-between-infective-factors-and-antibiotic-resistance-in-enterococci-clinical-isolates-in-west-of-iran
#15
Mohammad Reza Arabestani, Mona Nasaj, Seyed Masoud Mousavi
The present study was done to scrutinize the possible relation between infective genes and antimicrobial resistance in Enterococcus faecalis and Enterococcus faecium. Considering the fact that the presence of recognized infective determinants among clinical isolates may promote the emergence of infections and persistence of Enterococci in hospital settings, which can lead to an increase in antimicrobial resistance. 175 E. faecalis and 67 E. faecium isolated from clinical specimens were used. The isolates were identified, and then antibiotic susceptibility testing was performed...
January 2017: Chonnam Medical Journal
https://www.readbyqxmd.com/read/28177186/characterization-of-enterococcus-faecalis-isolated-from-the-cloaca-of-fancy-breeds-and-confined-chickens
#16
Steffen Lynge Jørgensen, Louise Ladefoged Poulsen, Lotte Thorndal, Amir Atabak Ronaghinia, Magne Bisgaard, Henrik Christensen
AIMS: The aim was to investigate the intestinal community of E. faecalis from healthy confined non-industrialized chicken. METHODS AND RESULTS: A total of 206 E. faecalis isolates were collected from cloacal swabs. The prevalence of E. faecalis from two confined flocks, were 83% and 96%, while only 44% and 13% in two fancy breeder flocks. A total of 204 isolates were characterized by pulsed-field-gel-electrophoresis (PFGE) where 40 strains were selected for multi locus sequence typing (MLST)...
February 8, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28169531/branimycins-b-and-c-antibiotics-produced-by-the-abyssal-actinobacterium-pseudonocardia-carboxydivorans-m-227
#17
Alfredo F Braña, Aida Sarmiento-Vizcaíno, Ignacio Pérez-Victoria, Luis Otero, Jonathan Fernández, Juan José Palacios, Jesús Martín, Mercedes de la Cruz, Caridad Díaz, Francisca Vicente, Fernando Reyes, Luis A García, Gloria Blanco
Two new antibiotics, branimycins B (2) and C (3), were produced by fermentation of the abyssal actinobacterium Pseudonocardia carboxydivorans M-227, isolated from deep seawater of the Avilés submarine Canyon. Their structures were elucidated by HRMS and NMR analyses. These compounds exhibit antibacterial activities against a panel of Gram-positive bacteria, including Corynebacterium urealyticum, Clostridium perfringens, and Micrococcus luteus, and against the Gram-negative bacterium Neisseria meningitidis...
February 7, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28167857/high-frequency-of-vancomycin-resistant-enterococcus-faecalis-in-children-an-alarming-concern
#18
F Sabouni, Z Movahedi, S Mahmoudi, B Pourakbari, S Keshavarz Valian, S Mamishi
INTRODUCTION: Enterococcus spp. is considered as important etiological agents of nosocomial infections. However, a little is known about the epidemiology of vancomycin resistant Enterococcus faecalis (VREF). The aim of this study was to investigate the frequency of VREF and detecting of two prevalent resistance genes (vanA, vanB) at Children Medical Center Hospital, an Iranian referral pediatric Hospital. MATERIALS AND METHODS: During January 2013 to December 2013, 180 E...
December 2016: Journal of Preventive Medicine and Hygiene
https://www.readbyqxmd.com/read/28167856/prevalence-of-high-level-gentamicin-resistant-enterococcus-faecalis-and-enterococcus-faecium-in-an-iranian-hospital
#19
M Emaneini, B Khoramian, F Jabalameli, R Beigverdi, K Asadollahi, M Taherikalani, A R Lari
This study was designed to determine the molecular characteristics and antimicrobial resistance of enterococcal strains isolated from patients admitted to an Iranian Hospital. Enterococcal strains were isolated from the burn patients. All strains were screened for genes encoding resistance to aminoglycoside [aac(6')-Ie-aph(2'')-Ia, aph (3'), ant (4')], resistance to vancomycin (vanA, vanB), resistance to tetracycline (tetK, tetL, tetM, tetO), and resistance to erythromycin (ermA, ermB, ermC) by PCR and multiplex PCR-based methods...
December 2016: Journal of Preventive Medicine and Hygiene
https://www.readbyqxmd.com/read/28167557/the-enterocin-as-48-as-an-evidence-for-the-use-of-bacteriocins-as-new-leishmanicidal-agents
#20
María Ángeles Abengózar, Rubén Cebrián, José María Saugar, Teresa Gárate, Eva Valdivia, Manuel Martínez-Bueno, Mercedes Maqueda, Luis Rivas
We report the feasibility of enterocin AS-48, a circular cationic peptide produced by Enterococcus faecalis, as a new leishmanicidal agent. AS-48 is lethal on Leishmania promastigotes as well as on axenic and intracellular amastigotes at low micromolar concentrations, with scarce cytotoxicity on macrophages. AS-48 induced a fast bioenergetic collapse of L. donovani promastigotes, but only a partial permeation of their plasma membrane with limited entrance of vital dyes, even at concentrations beyond its full lethality...
February 6, 2017: Antimicrobial Agents and Chemotherapy
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