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Enterococcus faecalis

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https://www.readbyqxmd.com/read/27910210/microbiological-evaluation-of-the-extension-wire-and-percutaneous-epidural-lead-anchor-site-following-a-2-stage-cut-down-spinal-cord-stimulator-procedure
#1
Abdul Ghaaliq Lalkhen, Mahindra Chincholkar, Jiten Patel
OBJECTIVES: There are concerns regarding bacterial colonization of the temporary extension leads and subsequent infection risk using the 2-stage cut-down approach in spinal cord stimulation (SCS). We sought to quantify the extent of bacterial colonization of the temporary extension wire and percutaneous epidural lead anchor site. MATERIALS AND METHODS: We conducted a cross-sectional observational study recruiting a pragmatic sample of 25 consecutive patients listed for a cut-down trial of SCS...
December 2, 2016: Pain Practice: the Official Journal of World Institute of Pain
https://www.readbyqxmd.com/read/27908405/biosynthesis-of-selenium-nanoparticles-using-enterococcus-faecalis-and-evaluation-of-their-antibacterial-activities
#2
Sara Shoeibi, Mohammad Mashreghi
Microorganisms are capable of synthesizing metal nanoparticles, and specifically Enterococcus faecalis bacteria were tested for its ability to synthesize selenium nanoparticles (Se-NPs) from sodium selenite. The biosynthesized Se-NPs were spherical in shape with the size range of 29-195nm. Also, the TEM microscopy showed the accumulation of nano-structures as extracellular deposits. The ability of the bacteria to tolerate high levels of toxic selenite was studied by changing with different concentrations of sodium selenite (0...
January 2017: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/27906556/combination-effect-of-cefuroxime-and-levofloxacin-against-bacteria-isolated-from-the-healthy-conjunctival-sac-and-endophthalmitis-cases-using-a-fractional-inhibitory-concentration-index
#3
Takashi Suzuki, Toshihiro Yamamoto, Tomohiko Torikai, Yuichi Ohashi
PURPOSE: Intracameral cefuroxime (CXM) and antibiotic eye drops such as fluoroquinolone are widely used for preventing endophthalmitis after cataract surgery. However, few reports are available regarding their interactions. This study was conducted to examine the in vitro interaction of CXM and levofloxacin (LVFX) against bacterial isolates from the healthy conjunctival sac before cataract surgery and from endophthalmitis cases. METHODS: The activity of each drug was determined using a broth dilution method...
December 1, 2016: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/27902396/determination-of-antimicrobial-resistance-of-enterococcus-strains-isolated-from-pigs-and-their-genotypic-characterization-by-method-of-amplification-of-dna-fragments-surrounding-rare-restriction-sites-the-adsrrs-fingerprinting
#4
Aneta Nowakiewicz, Grażyna Ziółkowska, Aleksandra Trościańczyk, Przemysław Zięba, Sebastian Gnat
In this study, we analysed phenotypic resistance profiles and their reflection in the genomic profiles of Enterococcus spp. strains isolated from pigs raised on different farms. Samples were collected from five pig farms (n=90 animals) and tested for Enterococcus. Minimum inhibitory concentrations of 12 antimicrobials were determined using the broth microdilution method, and epidemiological molecular analysis of strains belonging to selected species (E. faecalis, E. faecium and E. hirae) was performed using the ADSRRS-fingerprinting method with a few modifications...
November 28, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27895127/the-pathogenic-potential-of-proteus-mirabilis-is-enhanced-by-other-uropathogens-during-polymicrobial-urinary-tract-infection
#5
Chelsie E Armbruster, Sara N Smith, Alexandra O Johnson, Valerie DeOrnellas, Kathryn A Eaton, Alejandra Yep, Lona Mody, Weisheng Wu, Harry L T Mobley
Urinary catheter use is prevalent in health care settings, and polymicrobial colonization by urease-positive organisms, such as Proteus mirabilis and Providencia stuartii, commonly occurs with long-term catheterization. We previously demonstrated that coinfection with P. mirabilis and P. stuartii increased overall urease activity in vitro and disease severity in a model of urinary tract infection (UTI). In this study, we expanded these findings to a murine model of catheter-associated UTI (CAUTI), delineated the contribution of enhanced urease activity to coinfection pathogenesis, and screened for enhanced urease activity with other common CAUTI pathogens...
November 28, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27894963/longitudinal-surveillance-on-antibiogram-of-important-gram-positive-pathogens-in-southern-china-2001-to-2015
#6
Zhenbo Xu, Jinhong Xie, Brian M Peters, Bing Li, Lin Li, Guangchao Yu, Mark E Shirtliff
OBJECTIVES: A longitudinal surveillance aimed to investigate the antibiogram of three genus of important Gram-positive pathogens in Southern China during 2001-2015. METHODS: A total of 3849 Staphylococcus, Enterococcus and Streptococcus strains were isolated from Southern China during 2001-2015. Bacteria identification was performed by colony morphology, Gram staining, the API commercial kit and the Vitek 2 automated system. Antimicrobial susceptibility testing was determined by disk diffusion method and MIC method...
November 25, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27891459/comparison-of-antibacterial-efficacy-of-turmeric-extract-morinda-citrifolia-and-3-sodium-hypochlorite-on-enterococcus-faecalis-an-in-vitro-study
#7
Bathula Vimala Chaitanya, Kusum Valli Somisetty, Abhinav Diwan, Shiraz Pasha, Nandaprasad Shetty, Yashwanth Reddy, Shankar Nadigar
INTRODUCTION: Sodium hypochlorite (NaOCl), the most commonly used irrigant, has many potential properties like its unique ability to dissolve pulp tissue, excellent antimicrobial activity, but has a cytotoxic effect when injected into periapical tissues. It is also known to produce allergic reactions, foul smell and taste, and potential for corrosion. Facultative organisms such as Enterococcus faecalis and aerobes like Staphylococcus aureus are considered to be the most resistant species and one of the possible causes of root canal treatment failure...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27882119/bactericidal-effect-of-plasma-jet-with-helium-flowing-through-3-hydrogen-peroxide-against-enterococcus-faecalis
#8
Xin-Cai Zhou, Yu-Lan Li, De-Xi Liu, Ying-Guang Cao, Xin-Pei Lu
The aim of the present study was to assess the antimicrobial activity of plasma jet with helium (He) flowing through 3% hydrogen peroxide in root canals infected with Enterococcus faecalis. A total of 42 single-rooted anterior teeth were prepared, sterilized, inoculated with an E. faecalis suspension and incubated for 7 days. Next, the teeth were randomly divided into six experimental groups (including groups treated by plasma jet with or without He for different time durations) and one control group treated without plasma...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27881119/antibacterial-and-antifungal-potentials-of-the-solvents-extracts-from-eryngium-caeruleum-notholirion-thomsonianum-and-allium-consanguineum
#9
Abdul Sadiq, Sadiq Ahmad, Rahmat Ali, Fawad Ahmad, Sajjad Ahmad, Anwar Zeb, Muhammad Ayaz, Farhat Ullah, Abu Nasar Siddique
BACKGROUND: Herbal medicines have long been used for various ailments in various societies and natural bioactive compounds are gaining more and more importance due to various factors. In this context, three plant species i.e., Eryngium caeruleum, Notholirion thomsonianum and Allium consanguineum have been aimed for the scientific verification of their purported traditional uses against various infectious diseases. METHODS: In this study, three plants were assayed for antibacterial and antifungal potentials...
November 24, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27880799/treatment-of-oral-biofilms-by-a-d-enantiomeric-peptide
#10
Tian Zhang, Zhejun Wang, Robert E W Hancock, César de la Fuente-Núñez, Markus Haapasalo
Almost all dental diseases are caused by biofilms that consist of multispecies communities. DJK-5, which is a short D-enantiomeric, protease-resistant peptide with broad-spectrum anti-biofilm activity, was tested for its effect on oral multispecies biofilms. Peptide DJK-5 at 10 μg/mL effectively prevented the growth of these microbes in culture media in a time-dependent manner. In addition to the prevention of growth, peptide DJK-5 completely killed both Streptococcus mutans and Enterococcus faecalis suspended from biofilms after 30 minutes of incubation in liquid culture media...
2016: PloS One
https://www.readbyqxmd.com/read/27879993/randomized-clinical-trial-of-preoperative-skin-antisepsis-with-chlorhexidine-gluconate-or-povidone-iodine
#11
H M Park, S-S Han, E C Lee, S D Lee, H M Yoon, B W Eom, S H Kim, K W Ryu, S-J Park, Y W Kim, B Park
BACKGROUND: Skin antiseptic agents are used to prevent surgical-site infection (SSI); few trials have reported the superiority of any specific agent in clean-contaminated abdominal surgery. This RCT was designed to compare the effectiveness of chlorhexidine gluconate and povidone-iodine. METHODS: Consecutive patients who underwent clean-contaminated upper gastrointestinal or hepatobiliary-pancreatic open surgery between 2011 and 2014 were assigned randomly to either chlorhexidine gluconate or povidone-iodine...
November 23, 2016: British Journal of Surgery
https://www.readbyqxmd.com/read/27878401/enterocin-b3a-b3b-produced-by-lab-collected-from-infant-faeces-potential-utilization-in-the-food-industry-for-listeria-monocytogenes-biofilm-management
#12
Alaa Al-Seraih, Yanath Belguesmia, John Baah, Sabine Szunerits, Rabah Boukherroub, Djamel Drider
Enterococcus faecalis B3A-B3B produces the bacteriocin B3A-B3B with activity against Listeria monocytogenes, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Clostridium perfringens, but apparently not against fungi or Gram-negative bacteria, except for Salmonella Newport. B3A-B3B enterocin has two different nucleotides but similar amino acid composition to the class IIb MR10A-MR10B enterocin. B3A-B3B consists of two peptides of predicted molecular mass of 5176.31 Da (B3A) and 5182...
November 22, 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27872553/development-of-a-novel-test-for-simultaneous-bacterial-identification-and-antibiotic-susceptibility
#13
Jonathan Faro, Malika Mitchell, Yuh-Jue Chen, Sarah Kamal, Gerald Riddle, Sebastian Faro
Background. Elucidation of a pathogen's antimicrobial susceptibility requires subculture after the organism is first isolated. This takes several days, requiring patients to be treated with broad-spectrum antibiotics. This approach contributes to the development of bacterial resistance. Methods. Microtiter wells were coated with a polyclonal antibody targeting the pathogen of interest. Bacterial suspensions were added in the presence/absence of selected antibiotics. After washing, captured bacteria were detected...
2016: Infectious Diseases in Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27872201/identification-of-chemical-compounds-that-inhibit-protein-synthesis-in-pseudomonas-aeruginosa
#14
Stephanie O Palmer, Yanmei Hu, Megan Keniry, James M Bullard
Four inhibitory compounds were identified using a poly-uridylic acid (polyU) mRNA-directed aminoacylation/translation (A/T) protein synthesis system composed of phenylalanyl-tRNA synthetases (PheRS), ribosomes, and ribosomal factors from Pseudomonas aeruginosa in an in vitro screen of a synthetic compound library. The compounds were specific for inhibition of bacterial protein synthesis. In enzymatic assays, the compounds inhibited protein synthesis with IC50 values ranging from 20 to 60 μM. Minimum inhibitory concentrations (MICs) were determined in cultures for a panel of pathogenic organisms, including Enterococcus faecalis, Escherichia coli, Haemophilus influenzae, P...
November 21, 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27868361/synthesis-of-peptidoglycan-fragments-from-enterococcus-faecalis-with-fmoc-strategy-for-glycan-elongation
#15
Ning Wang, Hiroki Hasegawa, Cheng-Yuan Huang, Koichi Fukase, Yukari Fujimoto
Peptidoglycan (PGN) is an essential structural component of the bacterial cell wall conferring cell shape, which can be recognized by host-recognition proteins and receptors as well as bacterial surface proteins. In this work, the PGN partial structures from Enterococcus faecalis that contain a tetrasaccharide and an octasaccharide with a unique heptapeptide were synthesized via an Fmoc-strategy for elongation of the glycan chains. Namely, a 4'-O-Fmoc-protected disaccharide was utilized as the key intermediate in this efficient synthetic pathway for preparing various PGN fragments...
November 21, 2016: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/27867829/intrathecal-intraventricular-linezolid-in-multidrug-resistant-enterococcus-faecalis-ventriculitis
#16
Brian F Lich, Andrew K Conner, Joshua D Burks, Chad A Glenn, Michael E Sughrue
Background The use of intrathecal antibiotic therapy for the treatment of ventriculitis and/or meningitis has demonstrated efficacy especially when sterilization of the cerebrospinal fluid is not possible with intravenous antibiotics alone. Case Description We describe the successful treatment of Enterococcus faecalis ventriculitis utilizing intrathecal linezolid in a 32-year-old female patient with severe allergy to vancomycin, prohibitive bacterial susceptibilities, and failure of previous attempts to sterilize the cerebrospinal fluid despite multimodal treatment...
October 2016: Journal of Neurological Surgery Reports
https://www.readbyqxmd.com/read/27866468/intratubular-disinfection-with-tri-antibiotic-and-calcium-hydroxide-pastes
#17
Thais Cristina Pereira, Layla Reginna Silva Munhoz De Vasconcelos, Marcia Sirlene Zardin Graeff, Marco Antonio Hungaro Duarte, Clóvis Monteiro Bramante, Flaviana Bombarda De Andrade
OBJECTIVE: The aim of this study was to compare the in vitro intradentinal antimicrobial ability of the calcium hydroxide and tri-antibiotic pastes. MATERIALS AND METHODS: Standard bovine dentin tubes were sterilized and then infected with Enterococcus faecalis by a new contamination protocol of great depths of dentin. The specimens were filled with the medications, divided into two test-groups: calcium hydroxide (Group 1) and tri-antibiotic (Group 2) pastes. After 15 days, the teeth were evaluated by microbiological culture and confocal laser scanning microscopy (CLSM) with viability dye assay LIVE/DEAD inside dentinal tubules...
November 21, 2016: Acta Odontologica Scandinavica
https://www.readbyqxmd.com/read/27866000/effectiveness-of-final-decontamination-protocols-against-enterococcus-faecalis-and-its-influence-on-bond-strength-of-filling-material-to-root-canal-dentin
#18
Matheus Albino Souza, Daniel Dalla Lana, Ezequiel Gabrielli, Marlos Barbosa Ribeiro, Daniela Cristina Miyagaki, Doglas Cecchin
AIM: The aim of this study was to evaluate the effectiveness of final decontamination protocols against Enterococcus faecalis and their influence on bond strength of filling material to root canal dentin. MATERIAL AND METHODS: Ninety root canals were enlarged with ProTaper system and inoculated with E. faecalis for 15 days. Sixty samples were randomly divided into six groups (n=10) and subjected to following protocols: G1-distilled water(control), G2-2% chlorhexidine, G3-QMix, G4-6...
November 16, 2016: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/27865442/triterpene-glycosides-from-the-aerial-parts-of-gouania-longipetala
#19
Diane Patricia Apie Gossan, Abdulmagid Alabdul Magid, Philomène Akoua Yao-Kouassi, Antoine Ahibo Coffy, Jérome Josse, Sophie C Gangloff, Hamid Morjani, Laurence Voutquenne-Nazabadioko
Six previously undescribed triterpenoid saponins, gouaniaside I-VI, were isolated from the aerial parts of Gouania longipetala Hemsl. (Rhamnaceae), in addition to four known triterpenes. The structure elucidation of these compounds was based on analyses of spectroscopic data including 1D- and 2D-NMR and HR-ESI-MS techniques. The inhibitory activity of isolated compounds against promyelocytic leukemia HL60 and human erythromyeloblastoid leukemia K562 cell lines was evaluated and jujuboside I exhibited moderate cytotoxicity, with IC50 values of 13...
November 16, 2016: Phytochemistry
https://www.readbyqxmd.com/read/27862515/coevolutionary-dynamics-between-a-defensive-microbe-and-a-pathogen-driven-by-fluctuating-selection
#20
Suzanne A Ford, David Williams, Steve Paterson, Kayla C King
Microbes that protect their hosts from pathogenic infection are widespread components of the microbiota of both plants and animals. It has been found that interactions between 'defensive' microbes and pathogens can be genotype-specific and even underlie the variation in host resistance to pathogenic infection. These observations suggest a dynamic coevolutionary association between pathogens and defensive microbes, but direct evidence of coevolution is lacking. We tested the hypothesis that defensive microbes and pathogens could coevolve within host populations by co-passaging a microbe with host-defensive properties (Enterococcus faecalis) and a pathogen (Staphylococcus aureus) within Caenorhabditis elegans nematodes...
November 8, 2016: Molecular Ecology
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