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Enterococcus faecalis

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https://www.readbyqxmd.com/read/28545445/identification-of-enterococcus-faecalis-enzymes-with-azoreductases-and-or-nitroreductase-activity
#1
Valérie Chalansonnet, Claire Mercier, Sylvain Orenga, Christophe Gilbert
BACKGROUND: Nitroreductases, NAD(P)H dependent flavoenzymes, are found in most of bacterial species. Even if Enterococcus faecalis strains seems to present such activity because of their sensitivity to nitrofurans, no enzyme has been described. Nitroreductases were separated of others reductases due to their capacity to reduce nitro compounds. They are further classified based on their preference in cofactor: NADH and/or NADPH. However, recently, azoreductases have been studied for their strong activity on nitro compounds, especially nitro pro-drugs...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28543032/influence-of-different-wild-garlic-allium-ursinum-extracts-on-the-gastrointestinal-system-spasmolytic-antimicrobial-and-antioxidant-properties
#2
Dragana R Pavlović, Milica Veljković, Nikola M Stojanović, Marija Gočmanac-Ignjatović, Tatjana Mihailov-Krstev, Suzana Branković, Dušan Sokolović, Mirjana Marčetić, Niko Radulović, Mirjana Radenković
OBJECTIVES: As there are no previous studies of the European wild-garlic (Allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. METHODS: Wild-garlic extracts were tested for spasmolytic activity on isolated rat ileum, antimicrobial activity on selected Gram-positive and Gram-negative bacteria and fungi by microdilution method and antioxidant capacity by DPPH radical-scavenging assay...
May 23, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28542602/antimicrobial-resistance-status-of-enterococcus-from-australian-cattle-populations-at-slaughter
#3
Robert S Barlow, Kate E McMillan, Lesley L Duffy, Narelle Fegan, David Jordan, Glen E Mellor
Antimicrobial agents are used in cattle production systems for the prevention and control of bacterial associated diseases. A consequence of their use is the potential development of antimicrobial resistance (AMR). Enterococcus faecium and Enterococcus faecalis that are resistant to antimicrobials are of increased concern to public health officials throughout the world as they may compromise the ability of various treatment regimens to control disease and infection in human medicine. Australia is a major exporter of beef; however it does not have an ongoing surveillance system for AMR in cattle or foods derived from these animals...
2017: PloS One
https://www.readbyqxmd.com/read/28541565/emergence-of-vana-enterococcus-faecium-in-denmark-2005-15
#4
Anette M Hammerum, Sharmin Baig, Yasmin Kamel, Louise Roer, Mette Pinholt, Heidi Gumpert, Barbara Holzknecht, Bent Røder, Ulrik S Justesen, Jurgita Samulioniené, Mona Kjærsgaard, Claus Østergaard, Anette Holm, Esad Dzajic, Turid Snekloth Søndergaard, Shahin Gaini, Petra Edquist, Erik Alm, Berit Lilje, Henrik Westh, Marc Stegger, Henrik Hasman
Objectives: To describe the changing epidemiology of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis in clinical samples in Denmark 2005-15 according to species and van type, and, furthermore, to investigate the genetic relatedness of the clinical E. faecium isolates from 2015. Methods: During 2005-14, all clinical VRE isolates were tested for the presence of vanA/B/C genes by PCR. In 2015, all clinical VRE isolates were whole-genome sequenced...
May 25, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28536572/the-bacteriophage-ef-p29-efficiently-protects-against-lethal-vancomycin-resistant-enterococcus-faecalis-and-alleviates-gut-microbiota-imbalance-in-a-murine-bacteremia-model
#5
Mengjun Cheng, Jiaming Liang, Yufeng Zhang, Liyuan Hu, Pengjuan Gong, Ruopeng Cai, Lei Zhang, Hao Zhang, Jinli Ge, Yalu Ji, Zhimin Guo, Xin Feng, Changjiang Sun, Yongjun Yang, Liancheng Lei, Wenyu Han, Jingmin Gu
Enterococcus faecalis is becoming an increasingly important opportunistic pathogen worldwide, especially because it can cause life-threatening nosocomial infections. Treating E. faecalis infections has become increasingly difficult because of the prevalence of multidrug-resistant E. faecalis strains. Because bacteriophages show specificity for their bacterial hosts, there has been a growth in interest in using phage therapies to combat the rising incidence of multidrug-resistant bacterial infections. In this study, we isolated a new lytic phage, EF-P29, which showed high efficiency and a broad host range against E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28530185/effect-of-gnp-functionalisation-and-multiple-n-methylation-of-%C3%AE-amyloid-residue-32-37-on-gram-positive-bacterium
#6
Nanjundaswamy Marishetty Hemmaragala, Heidi Abrahamse, Blassan P George
In the previous report, the authors showed the gold nanoparticle (GNP) functionalised multiple N-methylated fragments of the residue (32-37) of beta (β)-amyloid protein (1-42), CGGIGLMVG and CGGGGGIGLMVG toward disruption of β-amyloid (1-42), the predominant component of senile plaques. Herein the in vitro antimicrobial activities of both normal and multiple N-methylated sequences of CGGIGLMVG and CGGGGGIGLMVG were screened and it was found that all the eight sequences including four (non-functionalised with GNP) to possess activity against both Gram-positive [Staphylococcus aureus (ATCC 43300) and Enterococcus faecalis (ATCC 5129)] and Gram-negative [Escherichia coli (ATCC 35218), Pseudomonas aeruginosa (ATCC 27853) and Klebsiella pneumoniae (ATCC 700603)] bacteria...
June 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/28527125/diversity-and-probiotic-potential-of-lactic-acid-bacteria-isolated-from-horreh-a-traditional-iranian-fermented-food
#7
Alireza Vasiee, Behrooz Alizadeh Behbahani, Farideh Tabatabaei Yazdi, Seyed Ali Mortazavi, Hamid Noorbakhsh
The aim of this study was to evaluate the probiotic potential of lactic acid bacteria (LAB) strains isolated from Horreh. Some probiotic properties, e.g., resistance to acid, bile tolerance, antibacterial activity, and antibiotic susceptibility, were investigated. A total of 140 Gram-positive and catalase-negative isolates from Horreh were subjected to identification and grouping by cultural methods and the 16S rRNA sequencing. The new isolates were identified to be Lactobacillus (fermentum, plantarum, and brevis) Weissella cibaria, Enterococcus (faecium and faecalis), Leuconostoc (citreum and mesenteroides subsp...
May 19, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28525287/occurrence-of-enterococcus-faecalis-and-enterococcus-faecium-in-various-clinical-infections-detection-of-their-drug-resistance-and-virulence-determinants
#8
Ali Jahansepas, Mohammad Aghazadeh, Mohammad Ahangarzadeh Rezaee, Alka Hasani, Yaeghob Sharifi, Toofan Aghazadeh, Jalal Mardaneh
The aim of this study was to characterize virulence determinants and antibiotic resistance profiles in enterococci obtained from various clinical sources in the northwest of Iran. A total of 160 enterococcal clinical isolates from various wards of University Teaching Hospitals were collected and specified by biochemical test, from September 2014 to July 2015. Identification of enterococci was confirmed by multiplex PCR in the genus and species level. Antibiotic resistance properties and virulence determinants were examined by phenotypic and molecular methods...
May 19, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28523739/mechanisms-of-peptide-sex-pheromone-regulation-of-conjugation-in-enterococcus-faecalis
#9
Yuqing Chen, Arpan Bandyopadhyay, Briana K Kozlowicz, Heather A H Haemig, Albert Tai, Wei-Shou Hu, Gary M Dunny
In many gram positive bacteria, horizontal transfer and virulence are regulated by peptide-mediated cell-cell signaling. The heptapeptide cCF10 (C) activates conjugative transfer of the Enterococcus faecalis plasmid pCF10, whereas the iCF10 (I) peptide inhibits transfer. Both peptides bind to the same domain of the master transcription regulator PrgX, a repressor of transcription of the prgQ operon encoding conjugation genes. We show that repression of prgQ by PrgX tetramers requires formation of a pCF10 DNA loop where each of two PrgX DNA-binding sites is occupied by a dimer...
May 19, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28515717/the-leaderless-bacteriocin-enterocin-k1-is-highly-potent-against-enterococcus-faecium-a-study-on-structure-target-spectrum-and-receptor
#10
Kirill V Ovchinnikov, Per Eugen Kristiansen, Daniel Straume, Marianne S Jensen, Tamara Aleksandrzak-Piekarczyk, Ingolf F Nes, Dzung B Diep
Enterocin K1 (EntK1), enterocin EJ97 (EntEJ97), and LsbB are three sequence related leaderless bacteriocins. Yet LsbB kills only lactococci while EntK1 and EntEJ97 target wider spectra with EntK1 being particularly active against Enterococcus faecium, including nosocomial multidrug resistant isolates. NMR study of EntK1 showed that it had a structure very similar to LsbB - both having an amphiphilic N-terminal α-helix and an unstructured C-terminus. The α-helix in EntK1 is, however, about 3-4 residues longer than that of LsbB...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28512652/comparison-of-simplistic-biofilm-models-for-%C3%A2-evaluating-irrigating-solutions
#11
Maximilian Gänsbauer, Andreas Burkovski, Matthias Karl, Tanja Grobecker-Karl
OBJECTIVE: Many innovations have recently been implemented in the field of endodontics, often based on a variety of in-vitro/ex-vivo test setups. It was the goal of this research to compare different biofilm models for evaluating the effectiveness of rinsing solutions. METHOD AND MATERIALS: Three different models have been applied in this study, including petri dishes with nutrient medium, arrays of human dentin disks, and split bovine root segments. The susceptibility of biofilms formed by Enterococcus faecalis to commonly used endodontic irrigants was tested...
May 16, 2017: Quintessence International
https://www.readbyqxmd.com/read/28512487/comparative-antimicrobial-efficacy-of-eucalyptus-galbie-and-myrtus-communis-l-extracts-chlorhexidine-and-sodium-hypochlorite-against-enterococcus-faecalis
#12
Mahdieh Nourzadeh, Arezu Amini, Farzaneh Fakoor, Maryam Raoof, Fariba Sharififar
INTRODUCTION: The aim of this study was to evaluate the antimicrobial effect of Eucalyptusgalbie and Myrtus communis L. methanolic extracts, chlorhexidine (CHX) and sodium hypochlorite (NaOCl) on Enterococcus faecalis (E. faecalis) as the predominant species isolated from infected root canals. METHODS AND MATERIALS: One hundred twenty mandibular premolars were randomly divided into 8 groups: Eucalyptusgalbie (E. galbie) 12.5 mg/mL, Myrtus communis L. (M. communis L...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/28511502/comparative-evaluation-of-three-herbal-solutions-on-the-disinfection-of-guttapercha-cones-an-in-vitro-study
#13
Pratap Kumar Mukka, Samba Shiva Rao Pola, Nanda Kumar Kommineni, Mayuri Sailaja Pachalla, Anand Ramtheerth Karne, Karthik Labishetty, Supriya Kommineni, Praveen Chilakabathini
INTRODUCTION: Guttapercha (GP) cones are usually supplied in aseptic, sealed packages from the manufacturers, but once exposed to the dental office environment or even by handling; they can be contaminated by a number of microorganisms. Supplementary decontamination of GP cones is critical. As they cannot be sterilized by moist or dry heat, cold sterilization, using disinfectants should be used. AIM: The aim of the study was to evaluate rapid, reliable, convenient and effective method of disinfection of GP cones using readily available herbal solutions such as Aloevera Juice, Amla Juice and Pancha Tulsi...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28511382/rapid-identification-of-vancomycin-resistant-enterococcus-faecalis-clinical-isolates-using-a-sugar-fermentation-method
#14
Javad Raeisi, Mahnaz Saifi, Mohammad Reza Pourshafie, Mehri Habibi, Hamid Reza Mohajerani, Neda Akbari, Mohammad Reza Asadi Karam
INTRODUCTION: Vancomycin Resistant Enterococci (VRE) can be found all over the world. Thus, rapid detection of the isolates could be of high importance in the treatment or prevention of the associated disease. AIM: To measure the turanose fermentation in Enterococcus faecalis clinical isolates for rapid differentiation of VRE and Vancomycin-Susceptible E. faecalis (VSE) isolates. MATERIALS AND METHODS: Forty E. faecalis samples were isolated from 200 clinical samples in Tehran Medical Center, Iran, from October 2012 to December 2012...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28510991/development-of-a-panel-of-recombinase-polymerase-amplification-assays-for-detection-of-common-bacterial-urinary-tract-infection-pathogens
#15
Balakrishnan Raja, Heather J Goux, Archana Marapadaga, Sri Rajagopalan, Katerina Kourentzi, Richard C Willson
AIMS: To develop and evaluate the performance of a panel of isothermal real-time recombinase polymerase amplification (RPA) assays for detection of common bacterial urinary tract infection (UTI) pathogens. METHODS AND RESULTS: The panel included RPAs for Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, and Enterococcus faecalis. All five RPAs required reaction times of under 12 minutes to reach their lower limit of detection of 100 genomes per reaction or less, and did not cross-react with high concentrations of non-target bacterial genomic DNA...
May 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28509506/anti-inflammatory-and-regenerative-effects-of-albanian-propolis-in-experimental-vital-amputations
#16
Aida Meto, Agron Meto, Bora Bimbari, Kastriot Shytaj, Mutlu Özcan
This study evaluated the effects of Albanian propolis on the inflamed pulpal tissue after pulpotomy in piglets. In five piglets, two teeth each were infected using special pathogenic microbial flora that was prepared in advance in order to cause inflammation of the pulp. Pulpotomy was performed in the maxillary and mandibular central and lateral incisors. Microbial flora pathogenesis prepared from the section of infected teeth, containing Staphylococcus aureus, Streptococcus viridans, Enterococcus faecalis, Staphylococcus albus and mixed flora were used to cause artificial pulpitis...
September 2016: European Journal of Prosthodontics and Restorative Dentistry
https://www.readbyqxmd.com/read/28508699/antimicrobial-anticancer-and-antioxidant-compounds-from-premna-resinosa-growing-in-saudi-arabia
#17
Dina A Albadawi, Ramzi A Mothana, Jamal M Khaled, Abdelkhader E Ashour, Ashok Kumar, Sheikh F Ahmad, Mansour S Al-Said, Adnan J Al-Rehaily, Nawal M Almusayeib
CONTEXT: Premna resinosa (Hochst.) Schauer (Lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage. OBJECTIVE: This study evaluates the anticancer, antimicrobial and antioxidant activities of P. resinosa, and isolates some responsible constituents. MATERIALS AND METHODS: The methanol extract of P. resinosa aerial parts and its fractions (n-hexane, dichloromethane, ethyl acetate and n-butanol) were tested...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28508523/the-effect-of-sodium-hypochlorite-concentration-and-irrigation-needle-extension-on-biofilm-removal-from-a-simulated-root-canal-model
#18
Saifalarab A Mohmmed, Morgana E Vianna, Matthew R Penny, Stephen T Hilton, Jonathan C Knowles
To investigate the effect of sodium hypochlorite concentration and needle extension on removal of Enterococcus faecalis biofilm, sixty root canal models were 3D printed. Biofilms were grown on the apical 3 mm of the canal for 10 days. Irrigation for 60s with 9 mL of either 5.25% or 2.5% NaOCl or water was performed using a needle inserted either 3 or 2 mm from the canal terminus and imaged using fluorescence microscopy and residual biofilm percentages were calculated using imaging software. The data were analysed using analysis of covariance and two-sample t-tests...
May 16, 2017: Australian Endodontic Journal: the Journal of the Australian Society of Endodontology Inc
https://www.readbyqxmd.com/read/28507786/application-of-hyperosmotic-nanoemulsions-in-wound-healing-partial-thickness-injury-model-in-swine
#19
Sean Connell, Jianming Li, Abigail Durkes, Lynetta Freeman
Objective: In this work, we introduce a novel hyperosmotic nanoemulsion (HNE) topical agent for use in wound healing. These topical emulsion complexes combine a lipophilic thymol nanoemulsion with a hyperosmotic saccharide matrix. This combination has been previously shown to possess synergistic antimicrobial activity against a host of common and drug-resistant pathogens in vitro. Approach: In this study, we present additional data to assess the safety and efficacy of these emulsions in a partial thickness injury model in swine...
May 1, 2017: Advances in Wound Care
https://www.readbyqxmd.com/read/28507488/microbiological-analysis-of-acute-infections-of-the-nail-fold-on-the-basis-of-bait-thread-test
#20
Hanna Tomczak, Aleksandra Dańczak-Pazdrowska, Adriana Polańska, Agnieszka Osmola-Mańkowska, Jakub Pazdrowski, Wioleta Błażejewska-Gąsior, Anna Horla, Marta Hasse-Cieślińska, Zygmunt Adamski
INTRODUCTION: An acute infection of the nail fold, called paronychia, is a common clinical problem. The basis for the implementation of the treatment is the result of microbiological examination. Due to the rapid and painful course of infection, usually an empirical antimicrobial treatment prior to obtaining microbiological test results is introduced. AIM: The microbial analysis of acute infections of the nail fold. MATERIAL AND METHODS: The study included 32 tests conducted on 31 patients of the Department of Dermatology...
April 2017: Postȩpy Dermatologii i Alergologii
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