keyword
MENU ▼
Read by QxMD icon Read
search

Adipose stem cells

keyword
https://www.readbyqxmd.com/read/29909342/fak-and-bmp-9-synergistically-trigger-osteogenic-differentiation-and-bone-formation-of-adipose-derived-stem-cells-through-enhancing-wnt-%C3%AE-catenin-signaling
#1
Cheng Yuan, Xiaoli Gou, Jiang Deng, Zhijun Dong, Peng Ye, Zhenming Hu
BACKGROUNDS: Adipose derived stem cells (ADSCs) could undergo osteogenesis via focal adhesion kinase (FAK) and bone morphogenetic protein (BMP) 9 signals, both of which could affect Wnt-β-catenin signal, a signal pathway closely related to ADSCs osteogenesis. It's still enigma whether FAK and BMP-9 contribute to osteogenesis. Here, we examined the effect of FAK on BMP9-inducedosteogenic differentiation, unveiled the possible molecular mechanism underling this process. METHODS: In the present study, ADSCs were isolated and purified, and cells of passage 3 underwent virus mediated transfection to prepare ADSCs with stable FAK shRNA expression...
June 14, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29908367/cell-proliferation-and-anti-apoptosis-essential-processes-for-recruitment-of-the-full-thermogenic-capacity-of-brown-adipose-tissue
#2
REVIEW
Jan Nedergaard, Yanling Wang, Barbara Cannon
In mice living under normal animal house conditions, the brown adipocytes in classical brown adipose tissue depots are already essentially fully differentiated: UCP1 mRNA and UCP1 protein levels are practically saturated. This means that any further recruitment - in response to cold exposure or any other browning agent - does not result in significant augmentation of these parameters. This may easily be construed to indicate that classical brown adipose tissue cannot be further recruited. However, this is far from the case: the capacity for further recruitment instead lies in the ability of the tissue to increase the number of brown-fat cells, a remarkable and highly controlled physiological recruitment process...
June 13, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29908226/visfatin-alters-the-cytokine-and-matrix-degrading-enzyme-profile-during-osteogenic-and-adipogenic-msc-differentiation
#3
Lali Tsiklauri, Janina Werner, Marian Kampschulte, Klaus W Frommer, Lucija Berninger, Martina Irrgang, Kristina Glenske, Dirk Hose, Thaqif El Khassawna, Jörn Pons-Kühnemann, Stefan Rehart, Sabine Wenisch, Ulf Müller-Ladner, Elena Neumann
OBJECTIVES: Age-related bone loss is associated with bone marrow adiposity. Adipokines (e.g. visfatin, resistin, leptin) are adipocyte-derived factors with immunomodulatory properties and might influence differentiation of bone marrow-derived mesenchymal stem cells (MSC) in osteoarthritis (OA) and osteoporosis. Thus, the presence of adipokines and MMPs in bone marrow and their effects on MSC differentiation were analyzed. METHODS: MSC and RNA were isolated from femoral heads after hip replacement surgery of OA or osteoporotic femoral neck fracture (FF) patients...
June 13, 2018: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/29905790/characterization-of-the-tissue-and-stromal-cell-components-of-micro-superficial-enhanced-fluid-fat-injection-micro-seffi-for-facial-aging-treatment
#4
Martina Rossi, Barbara Roda, Silvia Zia, Ilaria Vigliotta, Chiara Zannini, Francesco Alviano, Laura Bonsi, Andrea Zattoni, Pierluigi Reschiglian, Alessandro Gennai
Background: New microfat preparations provide material suitable for use as a regenerative filler for different facial areas. To support the development of new robust techniques for regenerative purposes, the cellular content of the sample should be considered. Objectives: To evaluate the stromal vascular fraction (SVF) cell components of micro-superficial enhanced fluid fat injection (SEFFI) samples via a technique to harvest re-injectable tissue with minimum manipulation...
June 14, 2018: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29905054/-research-of-acellular-xenogeneic-nerve-combined-with-adipose-derived-stem-cells-and-platelet-rich-plasma-in-repair-of-rabbit-facial-nerve-injury
#5
Yanna Sun, Rongming Zhang, Xu Mao, Mengshu Zhang
Objective: To investigate the early effects of acellular xenogeneic nerve combined with adipose-derived stem cells (ADSCs) and platelet rich plasma (PRP) in repairing facial nerve injury in rabbits. Methods: The bilateral sciatic nerves of 15 3-month-old male Sprague-Dawley rats were harvested and decellularized as xenografts. The allogeneic ADSCs were extracted from the neck and back fat pad of healthy adult New Zealand rabbits with a method of digestion by collagenase type Ⅰ and the autologous PRP was prepared by two step centrifugation...
June 1, 2018: Chinese Journal of Reparative and Reconstructive Surgery
https://www.readbyqxmd.com/read/29905053/-effect-of-human-adipose-derived-stem-cells-on-pressure-ulcer-healing-in-mouse
#6
Chengliang Deng, Zhiyuan Liu, Yuanzhen Yao, Ruichi Liu, Zairong Wei, Dali Wang
Objective: To investigate the effect of human adipose-derived stem cells (hADSCs) on pressure ulcers in mouse. Methods: The subcutaneous adipose tissue from voluntary donation was harvested. Then the hADSCs were isolated and cultured by mechanical isolation combined with typeⅠcollagenase digestion. The 3rd generation cells were identified by osteogenic, adipogenic, chondrogenic differentiations and flow cytometry. The platelet rich plasma (PRP) from peripheral blood donated by healthy volunteers was prepared by centrifugation...
June 1, 2018: Chinese Journal of Reparative and Reconstructive Surgery
https://www.readbyqxmd.com/read/29904354/paracrine-anti-inflammatory-effects-of-adipose-tissue-derived-mesenchymal-stem-cells-in-human-monocytes
#7
Maria I Guillén, Julia Platas, María D Pérez Del Caz, Vicente Mirabet, Maria J Alcaraz
The inflammatory process is an essential phenomenon in the induction of immune responses. Monocytes are key effector cells during the inflammatory process. A wide range of evidence indicates that mesenchymal stem cells from adipose tissue (ASC) are endowed with immunomodulatory capacity. However, the interaction between ASC and monocytes in the innate immune response is not well understood. The aim of this work was to investigate the possible paracrine anti-inflammatory effects of ASC in human monocytes. Monocytes were isolated from buffy coats and ASC from fat of non-obese patients...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29902838/icariside-ii-promotes-the-differentiation-of-adipose-tissue-derived-stem-cells-to-schwann-cells-to-preserve-erectile-function-after-cavernous-nerve-injury
#8
Tao Zheng, Tian-Biao Zhang, Chao-Liang Wang, Wei-Xing Zhang, Dong-Hui Jia, Fan Yang, Yang-Yang Sun, Xiao-Ju Ding, Rui Wang
Icariside II (ICA II) is used in erectile dysfunction treatment. Adipose tissue-derived stem cells (ADSCs) are efficient at improving erectile function. This study aimed to explore the action mechanism of ADSCs in improving erectile function. ADSCs were isolated from the adipose tissues of rats. Cell proliferation was determined using the Cell Counting Kit-8 (CCK-8) assay. The expressions of mRNA and protein were determined separately through qRT-PCR and western blot. The endogenous expressions of related genes were regulated using recombinant plasmids and cell transfection...
June 14, 2018: Molecules and Cells
https://www.readbyqxmd.com/read/29902544/systematic-review-of-the-therapeutic-roles-of-adipose-tissue-in-dermatology
#9
REVIEW
Frances M Walocko, Ariel E Eber, Robert S Kirsner, Evangelos Badiavas, Keyvan Nouri
BACKGROUND: Adipose tissue has classically functioned as a filler in restoring facial volume. Adipose tissue is also rich in stem cells, which may have a role in regenerative medicine. OBJECTIVE: To summarize the literature on the clinical uses of adipose tissue in scarring, wound healing, and hair growth and determine if evidence exists for clinical practice changes in dermatology. METHODS: We utilized the Preferred Reporting Items for Systemic Reviews and Meta-Analysis (PRISMA) to conduct the review...
June 11, 2018: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/29901507/magnetic-resonance-spectroscopy-for-evaluating-the-effect-of-pulsed-electromagnetic-fields-on-marrow-adiposity-in-postmenopausal-women-with-osteopenia
#10
Shaojun Li, Hongning Jiang, Bo Wang, Mingjun Gu, Xia Bi, Ying Yin, Yu Wang
OBJECTIVE: Pulsed electromagnetic fields (PEMFs) could promote osteogenic differentiation and suppress adipogenic differentiation in bone mesenchymal stem cells ex vivo. However, data on the effect of PEMF on marrow adiposity in humans remain elusive. We aimed to determine the in vivo effect of PEMF on marrow adiposity in postmenopausal women using magnetic resonance spectroscopy. METHODS: Sixty-one postmenopausal women with osteopenia, aged 53 to 85 years, were randomly assigned to receive either PEMF treatment or placebo...
June 12, 2018: Journal of Computer Assisted Tomography
https://www.readbyqxmd.com/read/29901449/label-free-process-monitoring-of-3d-bioprinted-engineered-constructs-via-dielectric-impedance-spectroscopy
#11
Lokesh Karthik Narayanan, Trevor L Thompson, Rohan A Shirwaiker, Binil Starly
Biofabrication processes can affect biological quality attributes of encapsulated cells within constructs. Currently, assessment of the fabricated constructs is performed offline by subjecting the constructs to destructive assays that require staining and sectioning. This drawback limits the translation of biofabrication processes to industrial practice. In this work, we investigate the dielectric response of viable cells encapsulated in bioprinted 3D hydrogel constructs to an applied alternating electric field as a label-free non-destructive monitoring approach...
June 14, 2018: Biofabrication
https://www.readbyqxmd.com/read/29901424/phenotyping-the-microvasculature-in-critical-sized-calvarial-defects-via-multimodal-optical-imaging
#12
Adam Mendez, Alexandra N Rindone, Namrata Batra, Pegah Abassnia, Janaka Senarathna, Stacy Gil, Darian H Hadjiabadi, Warren L Grayson, Arvind P Pathak
Tissue engineered scaffolds are a powerful means of healing craniofacial bone defects arising from trauma or disease. Murine models of critical-sized bone defects are especially useful in understanding the role of microenvironmental factors such as vascularization on bone regeneration. Here we demonstrate the capability of a novel multimodality imaging platform capable of acquiring in vivo images of microvascular architecture, microvascular blood flow and tracer/cell tracking via intrinsic optical signaling (IOS), laser speckle contrast (LSC) and fluorescence imaging, respectively in a critical-sized calvarial defect model...
June 14, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29901284/application-of-tissue-engineered-pericardial-patch-in-rat-models-of-myocardial-infarction
#13
Maryam Kameli, Reza Khorramirouz, Sahar Eftekharzadeh, Kiarad Fendereski, Seyedeh Sima Daryabari, Seyed Mohammad Tavangar, Abdol-Mohammad Kajbafzadeh
INTRODUCTION: Myocardial infarction (MI) is a major cause of mortality and morbidity in industrialized societies. Myocardial tissue engineering is an alternative and promising approach for substituting injured myocardium through development and seeding of appropriate scaffolds. In this study, we investigated the efficacy of using an acellular pericardium to deliver autologous mesenchymal stem cells (MSCs) to the infarcted site for regeneration of the myocardium. MATERIALS AND METHODS: MI was induced in two groups of rats; G1 or MI group, and G2 or patch-implanted group...
June 14, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29901210/completely-defined-co-culture-of-adipogenic-differentiated-adipose-derived-stem-cells-and-microvascular-endothelial-cells
#14
Ann-Cathrin Volz, Larissa Hack, Franziska B Atzinger, Petra J Kluger
Vascularized adipose tissue models are highly demanded as alternative to existing animal models to elucidate the mechanisms of widespread diseases, screen for new drugs or asses corresponding safety levels. Standardly used animal-derived sera therein, are associated to ethical concerns, the risk of contaminations and many uncertainties in their composition and impact on cells. Therefore their use should be completely omitted. In this study we developed a serum-free, defined co-culture medium and implemented it to set up an adipocyte-endothelial cell (EC) co-culture model...
June 13, 2018: ALTEX
https://www.readbyqxmd.com/read/29900517/mesenchymal-stem-cells-in-osteotomy-repair-after-tibial-tuberosity-advancement-in-dogs-with-cranial-cruciate-ligament-injury
#15
Clarissa Rocha Dos Santos, Richard da Rocha Filgueiras, Patrícia Furtado Malard, Andre Rodrigues da Cunha Barreto-Vianna, Kaique Nogueira, Carolina da Silva Leite, Eduardo Maurício Mendes de Lima
BACKGROUND: The cranial cruciate ligament rupture (CCLR) is the most commonly encountered orthopedic condition in dogs. Among the various techniques to treat this condition, tibial tuberosity advancement (TTA) has been used to obtain rapid recovery of the affected knee. The objective of this study was to evaluate the viability of the use of mesenchymal stem cells (MSC) implanted in the osteotomy site obtained by TTA in nine dogs diagnosed with CCLR. METHODS: The MSC were isolated from the adipose tissue of the dogs and cultured for eight days, the animals were divided into two groups...
June 14, 2018: Journal of Experimental Orthopaedics
https://www.readbyqxmd.com/read/29898789/co-stimulation-of-lpar-1-and-s1pr-1-3-increases-the-transplantation-efficacy-of-human-mesenchymal-stem-cells-in-drug-induced-and-alcoholic-liver-diseases
#16
Mianhuan Li, Yi Lv, Feng Chen, Xiaoyan Wang, Jiang Zhu, Hao Li, Jia Xiao
BACKGROUND: One of the major obstacles facing stem cell therapy is the limited number of functional stem cells available after transplantation due to the harsh microenvironment surrounding the damaged tissue. The aim of this study was to delineate the mechanistic involvement of lysophosphatidic acid receptors (LPARs) and sphingosine-1-phosphate receptors (S1PRs) in the regulation of anti-stress and transplantation efficacy of stem cells. METHODS: Human adipose-derived mesenchymal stem cells (hADMSCs) were treated with chemical toxin or ethanol to induce cell stress...
June 14, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29898623/depletion-of-sirt3-leads-to-the-impairment-of-adipogenic-differentiation-and-insulin-resistance-via-interfering-mitochondrial-function-of-adipose-derived-human-mesenchymal-stem-cells
#17
Yu-Ting Wu, Kun-Ting Chi, Yueh-Wen Lan, Jui-Chi Chan, Yi-Shing Ma, Yau-Huei Wei
Upregulation of mitochondrial function and oxidative metabolism is a hallmark in the differentiation of stem cells. However, the mechanism underlying the metabolic reprogramming and upregulation of mitochondrial function during the differentiation of human mesenchymal stem cells (hMSCs) is largely unclear. Sirt3 has emerged as a sensor in regulating mitochondrial function and antioxidant defense system in cellular response to energy demand or environmental stimuli, but its roles in stem cell differentiation has not been fully understood...
June 14, 2018: Free Radical Research
https://www.readbyqxmd.com/read/29895333/exosomes-secreted-by-adipose-derived-mesenchymal-stem-cells-regulate-type-i-collagen-metabolism-in-fibroblasts-from-women-with-stress-urinary-incontinence
#18
Xiaochun Liu, Shiwei Wang, Suhui Wu, Qian Hao, Ying Li, Zhuodan Guo, Wenzhen Wang
BACKGROUND: Mesenchymal stem cells (MSC) have gained credibility as a therapeutic tool partly due to their potential to secrete factors such as cytokines and chemokines. Recently, exosomes, which mediate intercellular communication by delivering biomolecules such as mRNA and miRNA into recipient cells, have gained attention as a new and valuable therapeutic strategy in regenerative medicine. However, the potential role of exosomes secreted by adipose-derived mesenchymal stem cells (adMSC-Exos) in collagen metabolism is not well understood...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29894435/concentrated-growth-factor-enhanced-fat-graft-survival-a-comparative-study
#19
Yun Hu, Yichen Jiang, Muyao Wang, Weidong Tian, Hang Wang
BACKGROUND: Concentrated growth factors (CGFs) belong to a new generation biomaterials that concentrate large number of growth factors and CD34 stem cells in small volume of plasma. OBJECTIVE: The purpose of this study was to evaluate the impact of the new technique, CGF, on fat graft survival, which compared with platelet-rich plasma (PRP) and platelet-rich fibrin (PRF). MATERIALS AND METHODS: Nude mice received fat graft were divided into PRP group, PRF group, CGF group, and saline...
June 8, 2018: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/29892899/microarray-analysis-after-adipose-derived-mesenchymal-stem-cells-injection-in-monosodium-iodoacetate-induced-osteoarthritis-rats
#20
Jae Chul Lee
Osteoarthritis (OA) is a degenerative joint disease characterized by abrasion, and ultimately, destruction of the articular cartilage and trabecular bone loss. OA is still considered a devastating disease, which requires an aggressive therapeutic approach. Despite the therapeutic potential of human adipose-derived mesenchymal stem cells (AD-MSCs), the molecular parameters needed to define the "stemness" remain largely unknown. Using high-density oligonucleotide microarrays, the differential gene expression profiles between a fraction of human adipose-derived (AD) mononuclear cells and its MSC subpopulation were obtained...
January 2018: Genes & Genomics
keyword
keyword
29437
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"