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Adipose stem cells

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https://www.readbyqxmd.com/read/28821996/mesenchymal-stromal-stem-cell-separation-methods-concise-review
#1
REVIEW
Andreas Nicodemou, Lubos Danisovic
Mesenchymal stem (stromal) cells (MSCs) possess unique biological characteristics such as plasticity, long term self-renewal, secretion of various bioactive molecules and ability of active migration to the diseased tissues that make them unique tool for regenerative medicine, nowadays. Until now MSCs were successfully derived from many tissue sources including bone marrow, umbilical cord, adipose tissue, dental pulp etc. The crucial step prior to their in vitro expansion, banking or potential clinical application is their separation...
August 18, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28821457/spontaneous-mutation-of-dock7-results-in-lower-trabecular-bone-mass-and-impaired-periosteal-expansion-in-aged-female-misty-mice
#2
Phuong T Le, Kathleen A Bishop, David E Maridas, Katherine J Motyl, Daniel J Brooks, Kenichi Nagano, Roland Baron, Mary L Bouxsein, Clifford J Rosen
Misty mice (m/m) have a loss of function mutation in Dock7 gene, a guanine nucleotide exchange factor, resulting in low bone mineral density, uncoupled bone remodeling and reduced bone formation. Dock7 has been identified as a modulator of osteoblast number and in vitro osteogenic differentiation in calvarial osteoblast culture. In addition, m/m exhibit reduced preformed brown adipose tissue innervation and temperature as well as compensatory increase in beige adipocyte markers. While the low bone mineral density phenotype is in part due to higher sympathetic nervous system (SNS) drive in young mice, it is unclear what effect aging would have in mice homozygous for the mutation in the Dock7 gene...
August 15, 2017: Bone
https://www.readbyqxmd.com/read/28819366/paracrine-potential-of-the-human-adipose-tissue-derived-stem-cells-to-modulate-balance-between-matrix-metalloproteinases-and-their-inhibitors-in-the-osteoarthritic-cartilage-in-vitro
#3
Jaroslav Denkovskij, Edvardas Bagdonas, Ilona Kusleviciute, Zygmunt Mackiewicz, Ausra Unguryte, Narunas Porvaneckas, Sandrine Fleury, Algirdas Venalis, Christian Jorgensen, Eiva Bernotiene
Adipose tissue represents an abundant source of stem cells. Along with anti-inflammatory effects, ASC secrete various factors that may modulate metabolism of extracellular matrix in osteoarthritic (OA) cartilage, suggesting that the presence of ASC could be advantageous for OA cartilage due to the recovery of homeostasis between matrix metalloproteinases (MMPs) and their tissue inhibitors of metalloproteinases (TIMPs). To evaluate these effects, cartilage explants (CE) were cocultured with ASC for 3 and 7 days under stimulation with or without IL-1β...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28818783/development-of-a-porous-3d-graphene-pdms-scaffold-for-improved-osseointegration
#4
Jianfeng Li, Xiao Liu, Jeremy M Crook, Gordon G Wallace
Osseointegration in orthopedic surgery plays an important role for bone implantation success. Traditional treatment of implant surface aimed at improved osseointegration has limited capability for its poor performance in supporting cell growth and proliferation. Polydimethylsiloxane (PDMS) is a widely used silicon-based organic polymer material with properties that are useful in cosmetics, domestic applications and mechanical engineering. In addition, the biocompatibility of PDMS, in part due to the high solubility of oxygen, makes it an ideal material for cell-based implants...
August 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28817698/correction-enhanced-hepatogenic-transdifferentiation-of-human-adipose-tissue-mesenchymal-stem-cells-by-gene-engineering-with-oct4-and-sox2
#5
Sei-Myoung Han, Ye-Rin Coh, Jin-Ok Ahn, Goo Jang, Soo Young Yum, Sung-Keun Kang, Hee-Woo Lee, Hwa-Young Youn
[This corrects the article DOI: 10.1371/journal.pone.0108874.].
2017: PloS One
https://www.readbyqxmd.com/read/28816826/tissue-engineered-bone-differentiated-from-human-adipose-derived-stem-cells-inhibit-posterolateral-fusion-in-an-athymic-rat-model
#6
Comron Saifi, Jonahtan Bernhard, Jamal N Shillingford, Petros Petridis, Samuel Robinson, X Edward Guo, Mark Weidenbaum, Ronald A Lehman, Howard S An, Lawrence G Lenke, Gordana Vunjak-Novakovic, Joseph L Laratta
STUDY DESIGN: Biological augmentation spinal arthrodesis trial in athymic rats OBJECTIVE.: To assess the efficacy of tissue-engineered bone to promote L4-L5 intertransverse process fusion in an athymic rat model. SUMMARY OF BACKGROUND DATA: Each year in the United States, over 400,000 spinal fusion surgeries are performed requiring bone graft. The current gold standard for posterolateral lumbar fusion is autogenous iliac crest bone graft (ICBG), but the harvesting of ICBG is associated with increased operative time and significant complications...
August 14, 2017: Spine
https://www.readbyqxmd.com/read/28816507/arthroscopic-harvest-of-adipose-derived-mesenchymal-stem-cells-from-the-infrapatellar-fat-pad
#7
Jason L Dragoo, Wenteh Chang
BACKGROUND: The successful isolation of adipose-derived mesenchymal stem cells (ADSCs) from the arthroscopically harvested infrapatellar fat pad (IFP) would provide orthopaedic surgeons with an autologous solution for regenerative procedures. PURPOSE: To demonstrate the quantity and viability of the mesenchymal stem cell population arthroscopically harvested from the IFP as well as the surrounding synovium. STUDY DESIGN: Descriptive laboratory study...
August 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28816316/hydrogel-scaffolds-for-differentiation-of-adipose-derived-stem-cells
#8
REVIEW
Qiutong Huang, Yijie Zou, Maria C Arno, Shuai Chen, Tao Wang, Jingyi Gao, Andrew P Dove, Jianzhong Du
Natural extracellular matrices (ECMs) have been widely used as a support for the adhesion, migration, differentiation, and proliferation of adipose-derived stem cells (ADSCs). However, poor mechanical behavior and unpredictable biodegradation properties of natural ECMs considerably limit their potential for bioapplications and raise the need for different, synthetic scaffolds. Hydrogels are regarded as the most promising alternative materials as a consequence of their excellent swelling properties and their resemblance to soft tissues...
August 17, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28816097/meltblown-polymer-fabrics-as-candidate-scaffolds-for-rotator-cuff-tendon-tissue-engineering
#9
Thomas Jenkins, Sean Meehan, Behnam Pourdeyhimi, Dianne Little
Various biomaterial technologies are promising for tissue engineering, including electrospinning, but commercial scale-up of throughput is difficult. The goal of the study was to evaluate meltblown fabrics as candidate scaffolds for rotator cuff tendon tissue engineering. Meltblown poly(lactic acid) fabrics were produced with several polymer crystallinities and airflow velocities (500(low), 900(medium) or 1400(high) m<sup>3</sup>air/hour/m fabric). Fiber diameter, alignment, and baseline bi-directional tensile mechanical properties were evaluated...
August 17, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28815865/bone-marrow-and-adipose-mesenchymal-stem-cells-attenuate-cardiac-fibrosis-induced-by-methotrexate-in-rats
#10
Ebtehal Mohammad Fikry, Wedad A Hassan, Amany M Gad
Mesenchymal stem cells (MSCs) are an ideal adult stem cell with capacity for self-renewal and differentiation with an extensive tissue distribution. The present study evaluates the therapeutic effects of bone marrow mesenchymal stem cells (BM-MSCs) or adipose-derived mesenchymal stem cells (AD-MSCs) against the development of methotrexate (MTX)-induced cardiac fibrosis versus dexamethasone (DEX). Rats were allocated into five groups; group 1, received normal saline orally; group 2, received MTX (14 mg/kg/week for 2 weeks); groups 3 and 4, treated once with 2 × 10(6) cells of MTX + BM-MSCs and MTX + AD-MSCs, respectively; and group 5, MTX + DEX (0...
August 16, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28814761/transplantation-of-bioengineered-rat-lungs-recellularized-with-endothelial-and-adipose-derived-stromal-cells
#11
Ryoichiro Doi, Tomoshi Tsuchiya, Norisato Mitsutake, Satoshi Nishimura, Mutsumi Matsuu-Matsuyama, Yuka Nakazawa, Tomoo Ogi, Sadanori Akita, Hiroshi Yukawa, Yoshinobu Baba, Naoya Yamasaki, Keitaro Matsumoto, Takuro Miyazaki, Ryotaro Kamohara, Go Hatachi, Hideyori Sengyoku, Hironosuke Watanabe, Tomohiro Obata, Laura E Niklason, Takeshi Nagayasu
Bioengineered lungs consisting of a decellularized lung scaffold that is repopulated with a patient's own cells could provide desperately needed donor organs in the future. This approach has been tested in rats, and has been partially explored in porcine and human lungs. However, existing bioengineered lungs are fragile, in part because of their immature vascular structure. Herein, we report the application of adipose-derived stem/stromal cells (ASCs) for engineering the pulmonary vasculature in a decellularized rat lung scaffold...
August 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28813021/enhanced-cell-growth-of-adipocyte-derived-mesenchymal-stem-cells-using-chemically-defined-serum-free-media
#12
Myung-Suk Lee, Christine Youn, Jeong Hyun Kim, Byoung Jun Park, Jongchan Ahn, Sungyoul Hong, Young-Deug Kim, Young Kee Shin, Sang Gyu Park
The multipotency and anti-inflammatory effects of mesenchymal stem cells (MSCs) make them attractive for cell therapy in regenerative medicine. A large number of MSCs is required for efficient therapy owing to the low homing efficiency of MSCs to target sites. Furthermore, owing to limitations in obtaining sufficient amounts of MSCs, in vitro expansion of MSCs that preserves their differentiation and proliferative potential is essential. The animal factor included in culture media also limits clinical application...
August 16, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28812231/the-neuroprotective-effect-of-conditioned-medium-from-human-adipose-derived-mesenchymal-stem-cells-is-impaired-by-n-acetyl-cysteine-supplementation
#13
Teodoro Palomares, María Cordero, Cristina Bruzos-Cidon, María Torrecilla, Luisa Ugedo, Ana Alonso-Varona
Oxidative stress is a common feature in neurodegenerative diseases associated with neuroinflammation, and therefore, has been proposed as a key target for novel therapies for these diseases. Recently, adipose-derived stem cell (ASC)-based cell therapy has emerged as a novel strategy for neuroprotection. In this study, we evaluate the therapeutic role of ASC-conditioned medium (ASC-CM) against H2O2-induced neurotoxicity in a new in vitro model of ec23/brain-derived neurotrophic factor (BDNF)-differentiated human SH-SY5Y neuron-like cells (SH-SY5Yd)...
August 15, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28812219/eminent-sources-of-adult-mesenchymal-stem-cells-and-their-therapeutic-imminence
#14
REVIEW
Dannie Macrin, Joel P Joseph, Aruthra Arumugam Pillai, Arikketh Devi
In the recent times, stem cell biology has garnered the attention of the scientific fraternity and the general public alike due to the immense therapeutic potential that it holds in the field of regenerative medicine. A breakthrough in this direction came with the isolation of stem cells from human embryo and their differentiation into cell types of all three germ layers. However, the isolation of mesenchymal stem cells from adult tissues proved to be advantageous over embryonic stem cells due to the ethical and immunological naivety...
August 15, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28811006/improved-fat-transplantation-survival-by-using-the-conditioned-medium-of-vascular-endothelial-growth-factor-transfected-human-adipose-derived-stem-cells
#15
Yang Zhang, Li-Ling Xiao, Jiang-Xuan Li, Hong-Wei Liu, Sheng-Hong Li, Yan-Yun Wu, Xuan Liao, Cong-Qiang Rao
Autologous fat transplantation has been applied widely in clinic. However, the low survival rate is still a problem to be solved. Studies shows that the human adipose-derived stem cells (ADSCs) transfected by vascular endothelial growth factor (VEGF) can improve the survival rate of autologous fat transplantation. Our study is to evaluate the effects of the conditioned medium of VEGF-transfected human adipose-derived stem cells (VEGF-ADSCs-CM) on fat transplantation. ADSCs were isolated and transfected with MOI = 40...
August 2017: Kaohsiung Journal of Medical Sciences
https://www.readbyqxmd.com/read/28810594/tissue-engineered-sling-with-adipose-derived-stem-cells-under-static-mechanical-strain
#16
Ying Wang, Wei Wang, Xilong Wang, Yangyun Wang, Jihong Wang, Qiang Fu, Guowei Shi
The implantation of a suburethral sling is an important treatment for stress urinary incontinence (SUI). However, the slings used current have a number of inherent limitations, such as tissue rejection and infection. The present study investigated the potential of engineering sling tissue in vitro using adipose-derived stem cells (ADSCs). The ADSCs were obtained from Sprague-Dawley rats and were characterized in vitro. The ADSCs were seeded on polyglycolic acid (PGA) fibers that formed a scaffold with a shape mimicking a sling complex...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28809824/creation-and-transplantation-of-an-adipose-derived-stem-cell-asc-sheet-in-a-diabetic-wound-healing-model
#17
Yuka Kato, Takanori Iwata, Kaoru Washio, Toshiyuki Yoshida, Hozue Kuroda, Shunichi Morikawa, Mariko Hamada, Kazuki Ikura, Nobuyuki Kaibuchi, Masayuki Yamato, Teruo Okano, Yasuko Uchigata
Artificial skin has achieved considerable therapeutic results in clinical practice. However, artificial skin treatments for wounds in diabetic patients with impeded blood flow or with large wounds might be prolonged. Cell-based therapies have appeared as a new technique for the treatment of diabetic ulcers, and cell-sheet engineering has improved the efficacy of cell transplantation. A number of reports have suggested that adipose-derived stem cells (ASCs), a type of mesenchymal stromal cell (MSC), exhibit therapeutic potential due to their relative abundance in adipose tissue and their accessibility for collection when compared to MSCs from other tissues...
August 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28808812/antioxidant-effects-of-chrysin-loaded-electrospun-nanofibrous-mats-on-proliferation-and-stemness-preservation-of-human-adipose-derived-stem-cells
#18
Yaghoub Deldar, Faraz Zarghami, Younes Pilehvar-Soltanahmadi, Mehdi Dadashpour, Nosratollah Zarghami
An ideal biomaterial in regenerative medicine should be able to regulate the stem cell proliferation without the loss of its pluripotency. Chrysin (Chr) is a naturally occurring flavone with a wide spectrum of biological functions including anti-inflammatory and anti-oxidant properties. The present study describes the influence of Chr-loaded nanofibrous mats on the regulation of proliferation and stemness preservation of adipose-derived stem cells (ADSCs). For this purpose, Chr-loaded poly (ε-caprolactone)/poly (ethylene glycol) (PCL/PEG) nanofibrous mats were produced via electrospinning process and the successful fabrication of these bioactive mats was confirmed by field emission scanning electron microscopy (FE-SEM) and fourier transform infrared spectroscopy...
August 14, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28808264/knockdown-of-lncrna-mir31hg-inhibits-adipocyte-differentiation-of-human-adipose-derived-stem-cells-via-histone-modification-of-fabp4
#19
Yiping Huang, Chanyuan Jin, Yunfei Zheng, Xiaobei Li, Shan Zhang, Yixin Zhang, Lingfei Jia, Weiran Li
Adipogenesis plays an important role in the regulation of whole-body energy homeostasis and is inextricably related to obesity. Several studies have highlighted the relevance of microRNAs in adipocyte differentiation, but the contributions of long non-coding RNAs (lncRNAs) are still largely uncharacterized. Here, we determined that lncRNA MIR31HG is related to adipocyte lineage commitment. We demonstrated that knockdown of MIR31HG inhibited adipocyte differentiation, whereas overexpression of MIR31HG promoted adipogenesis in vitro and in vivo...
August 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28807877/cryptotanshinone-promotes-commitment-to-the-brown-adipocyte-lineage-and-mitochondrial-biogenesis-in-c3h10t1-2-mesenchymal-stem-cells-via-ampk-and-p38-mapk-signaling
#20
Khan Mohammad Imran, Naimur Rahman, Dahyeon Yoon, Miso Jeon, Byong-Taek Lee, Yong-Sik Kim
Although white adipose tissue (WAT) stores triglycerides and contributes to obesity, brown adipose tissue (BAT) dissipates energy as heat. Therefore, browning of WAT is regarded as an attractive way to counteract obesity. Our previous studies have revealed that treatment with cryptotanshinone (CT) during adipogenesis of 3T3-L1 cells inhibits their differentiation. Here, we found that pretreatment of C3H10T1/2 mesenchymal stem cells with CT before exposure to adipogenic hormonal stimuli promotes the commitment of these mesenchymal stem cells to the adipocyte lineage as confirmed by increased triglyceride accumulation...
August 11, 2017: Biochimica et Biophysica Acta
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