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Adipose stem cells

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https://www.readbyqxmd.com/read/28109150/-isolation-culture-and-identification-of-adipose-derived-stem-cells-from-sd-rat-adipose-tissues-subjected-to-long-term-cryopreservation
#1
Qin Liu, Liping Wang, Fang Chen, Yi Zhang
Objective To study the feasibility of isolation and culture of adipose-derived stem cells (ADSCs) from SD rat adipose tissues subjected to long-term cryopreservation. Methods We took inguinal fat pads from healthy SD rats. Adipose tissues were stored with 100 mL/L dimethyl sulfoxide (DMSO) combined with 900 mL/L fetal bovine serum (FBS) in liquid nitrogen. Three months later, the adipose tissues were resuscitated for the isolation and culture of ADSCs. The growth status and morphology were observed. The growth curve and cell surface markers CD29, CD45, CD90 of the 3rd passage cells were analyzed respectively by CCK-8 assay and immunocytochemistry...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109140/-transplantation-of-bone-marrow-mesenchymal-stem-cell-improves-antioxidant-capacity-and-immune-activity-of-aging-model-rats
#2
Zhihong Wang, Yun Lin, Weimin Chen, Jin Shang, Tiannan Wei
Objective To investigate the impact of bone marrow mesenchymal stem cell (BMSC) transplantation on the antioxidant capacity and immune activity of aging rats induced by D-galactose. Methods Ten healthy male SD rats served as a control group (aged 2 months). To establish aging models, healthy SD rats were daily injected subcutaneously with D-galactose (400 mg/kg). Then the aging model rats were randomized into aging model group and BMSC group (ten rats in each group). The BMSC group was injected with 3×10(6) BMSCs via tail vein...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109058/in-vivo-construction-of-liver-tissue-by-implantation-of-a-hepatic-non-parenchymal-adipose-derived-stem-cell-sheet
#3
Mio Fujii, Kosho Yamanouchi, Yusuke Sakai, Zhassulan Baimakhanov, Izumi Yamaguchi, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Tamotsu Kuroki, Susumu Eguchi
Subcutaneous hepatocyte sheet implantation is an attractive therapeutic option for various liver diseases. However, this technique is limited by the availability of hepatocytes. Thus, the use of hepatic non-parenchymal cells (NPCs) containing small hepatocytes, which have the ability to proliferate more rapidly than mature hepatocytes, for transplantation has been suggested. The aim of our study was to construct liver tissue subcutaneously in rats by implanting NPC sheets co-cultivated with adipose-derived stem cells (ADSCs), which produce certain angiogenic factors...
January 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28108956/post-implantation-fate-of-adipogenic-induced-mesenchymal-stem-cells-on-type-i-collagen-scaffold-in-a-rat-model
#4
Balu Venugopal, Francis B Fernandez, V S Harikrishnan, Annie John
Regenerative medicine via its application in soft tissue reconstruction through novel methods in adipose tissue engineering (ATE) has gained remarkable attention and investment despite simultaneous reports on clinical incidence of graft resorption and impaired vascularization. The underlying malaise here once identified may play a critical role in optimizing implant function. Our work attempts to determine the fate of donor cells and the implant in recipient micro environment using adipose-derived mesenchymal stem cells (ASCs) on a type I collagen sponge, an established scaffold for ATE...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28108309/hypoxia-enhances-the-viability-growth-and-chondrogenic-potential-of-cryopreserved-human-adipose-derived-stem-cells
#5
Wan Kamarul Zaman Wan Safwani, Jane Ru Choi, Kar Wey Yong, Iris Ting, Noor Azmi Mat Adenan, Belinda Pingguan-Murphy
Cryopreservation is the only existing method of storage of human adipose-derived stem cells (ASCs) for clinical use. However, cryopreservation has been shown to be detrimental to ASCs, particularly in term of cell viability. To restore the viability of cryopreserved ASCs, it is proposed to culture the cells in a hypoxic condition. To this end, we aim to investigate the effect of hypoxia on the cryopreserved human ASCs in terms of not only cell viability, but also their growth and stemness properties, which have not been explored yet...
January 17, 2017: Cryobiology
https://www.readbyqxmd.com/read/28107865/adipose-tissue-derived-stem-cells-ameliorates-dermal-fibrosis-in-a-mouse-model-of-scleroderma
#6
Wei Chen, Zhi-Kuan Xia, Man-Hui Zhang, Gui-Chun Ding, Xiao-Yan Zhang, Zheng-Xu Wang, Rong-Ya Yang
OBJECTIVE: To investigate the therapeutic potential of adipose-derived stem cells (ADSCs) for limited cutaneous scleroderma (LS) in mouse models. METHODS: ADSCs were isolated from pathogen-free female C57BL/6 mice and LS was induced in wild type (WT) C57BL/6 mice via daily injection of bleomycin (0.1 mL × 300 μg/mL) for 4 weeks; then the ADSCs were subcutaneously injected into the dorsal area in the model treatment group, and 100 μL of phosphate-buffered saline (PBS) solution was injected into the same site in the model control group...
January 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28107808/in-situ-release-of-vegf-enhances-osteogenesis-in-3d-porous-scaffolds-engineered-with-osterix-modified-adipose-derived-stem-cells
#7
Wan-Lin Xu, Hui-Shan Ong, Yun Zhu, Sheng-Wen Liu, Li-Min Liu, Kai-Hua Zhou, Zeng-Qi Xu, Jun Gao, Yan Zhang, Jin-Hai Ye, Wen-Jun Yang
Adipose-derived stem cells (ADSCs) can differentiate into various cell types and thus have great potential for regenerative medicine. Herein, rat ADSCs were isolated; transduced with lentiviruses expressing Osterix (Osx), a transcriptional factor essential for osteogenesis. Osx overexpression up-regulated key osteogenesis-related genes, such as special AT-rich binding protein 2, alkaline phosphatase, osteocalcin and osteopontin, at both the mRNA and protein levels. In addition, mineral nodule formation and alkaline phosphatase activity were enhanced in Osx-overexpressing ADSCs...
January 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28107610/tonsil-derived-mesenchymal-stem-cells-t-mscs-prevent-th17-mediated-autoimmune-response-via-regulation-of-the-programmed-death-1-programmed-death-ligand-1-pd-1-pd-l1-pathway
#8
Ji-Yon Kim, Minhwa Park, Yu-Hee Kim, Kyung-Ha Ryu, Kyung Ho Lee, Kyung-Ah Cho, So-Youn Woo
Our knowledge of the immunomodulatory role of mesenchymal stem cells (MSCs) in both the innate and adaptive immune systems has dramatically expanded, providing great promise for treating various autoimmune diseases. However, the contribution of MSCs to Th17 dominant immune disease, such as psoriasis and its underlying mechanism remains elusive. In this study, we demonstrated that human palatine tonsil-derived MSCs (T-MSCs) constitutively express both the membrane-bound and soluble forms of programmed death-ligand 1 (PD-L1), which enables T-MSCs to be distinguished from MSCs originating from other organs (i...
January 20, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28106629/heat-shock-protein-70-overexpression-in-adipose-derived-stem-cells-enhances-fat-graft-survival
#9
Hao Feng, Lihong Qiu, Teng Zhang, Houyou Yu, Xianjie Ma, Yingjun Su, Hui Zheng, Yong Wang, Chenggang Yi
BACKGROUND: Autologous fat grafting is a prevalent technique used for soft-tissue augmentation; however, the poor survival rate of the grafted tissue remains a drawback of this method. Although adipose-derived stem cells (ASCs) are an attractive candidate for enhancing graft retention, the poor posttransplantation viability of these cells limits their application. Here we investigated whether overexpression of the antiapoptotic protein heat-shock protein 70 (Hsp70) could enhance ASCs' therapeutic potential for fat transplant survival...
January 19, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28106165/effects-of-sr-ht-gahnite-on-osteogenesis-and-angiogenesis-by-adipose-derived-stem-cells-for-critical-sized-calvarial-defect-repair
#10
Guifang Wang, Seyed-Iman Roohani-Esfahani, Wenjie Zhang, Kaige Lv, Guangzheng Yang, Xun Ding, Derong Zou, Daxiang Cui, Hala Zreiqat, Xinquan Jiang
Tissue engineering strategies to construct vascularized bone grafts are now attracting much attention. Strontium-hardystonite-Gahnite (Sr-HT-Gahnite) is a strong, highly porous, and biocompatible calcium silicate based bio-ceramic that contains strontium and zinc ions. Adipose derived stem cells (ASCs) have been demonstrated to have the ability in promoting osteogenesis and angiogenesis. In this study, the effects of Sr-HT-Gahnite on cell morphology, cell proliferation, and osteogenic differentiation of ASCs were systematically investigated...
January 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28106095/modeling-stem-cell-myogenic-differentiation
#11
Rajiv S Deshpande, Alexander A Spector
The process of stem cell myogenesis (transformation into skeletal muscle cells) includes several stages characterized by the expression of certain combinations of myogenic factors. The first part of this process is accompanied by cell division, while the second part is mainly associated with direct differentiation. The mechanical cues are known to enhance stem cell myogenesis, and the paper focuses on the stem cell differentiation under the condition of externally applied strain. The process of stem cell myogenic differentiation is interpreted as the interplay among transcription factors, targeted proteins and strain-generated signaling molecule, and it is described by a kinetic multi-stage model...
January 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28105339/analysis-of-multiple-types-of-human-cells-subsequent-to-bioprinting-with-electrospraying-technology
#12
Yu Xin, Gang Chai, Ting Zhang, Xiangsheng Wang, Miao Qu, Andy Tan, Melia Bogari, Ming Zhu, Li Lin, Qingxi Hu, Yuanyuan Liu, Yan Zhang
The aim of the present study was to investigate bioprinting with electrospraying technology using multiple types of human cell suspensions as bio-ink, in order to lay the initial foundations for the application of the bioprinting technology in tissue engineering. In the current study, six types of human cells were selected and cultured, including human fibroblasts, human adipose-derived stem cells (hADSCs), human periodontal ligament cells (HPDLCs), adult human retinal pigment epithelial cells (ARPE-19), human umbilical vascular endothelial cells (HUVECs) and human gastric epithelial cell line (GES-1)...
December 2016: Biomedical Reports
https://www.readbyqxmd.com/read/28103423/adipose-derived-perivascular-mesenchymal-stromal-stem-cells-promote-functional-vascular-tissue-engineering-for-cardiac-regenerative-purposes
#13
Justin Morrissette-McAlmon, Adriana Blazeski, Sarah Somers, Geran Kostecki, Leslie Tung, Warren L Grayson
Cardiac tissue engineering approaches have the potential to regenerate functional myocardium with intrinsic vascular networks. In this study, we compared the relative effects of human adipose-derived stem/stromal cells (hASCs) and human dermal fibroblasts (hDFs) in co-cultures with neonatal rat ventricular cardiomyocytes (NRVCMs) and human umbilical vein endothelial cells (HUVECs). We found that at the same ratios of NRVCM:hASC and NRVCM:hDF, the hASC co-cultures displayed shorter action potentials and maintained capture at faster pacing rates...
January 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28102844/human-adipose-stem-cell-differentiation-is-highly-affected-by-cancer-cells-both-in-vitro-and-in-vivo-implication-for-autologous-fat-grafting
#14
Francesca Paino, Marcella La Noce, Diego Di Nucci, Giovanni Francesco Nicoletti, Rosa Salzillo, Alfredo De Rosa, Giuseppe Andrea Ferraro, Gianpaolo Papaccio, Vincenzo Desiderio, Virginia Tirino
Recent studies showed that mesenchymal stem cells derived from adipose tissue can promote tumour progression, raising some concerns regarding their use in regenerative medicine. In this context, we co-cultured either SAOS2 osteosarcoma or MCF7 breast cancer cells with human adipose stem cells (hASCs), in order to evaluate potential effects of cancer cells on hASCs differentiation, in vitro and in vivo. In this study we observed that both SAOS2 and MCF7 cell lines induced an increase in hASCs proliferation, compared to hASCs alone, but, surprisingly, neither changes in the expression of CD90, CD29, CD324 and vimentin, nor variations in the Twist and Slug mRNAs were detectable...
January 19, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28102326/demonstration-of-the-protein-involvement-in-cell-electropermeabilization-using-confocal-raman-microspectroscopy
#15
Antoine Azan, Valérie Untereiner, Cyril Gobinet, Ganesh D Sockalingum, Marie Breton, Olivier Piot, Lluis M Mir
Confocal Raman microspectroscopy was used to study the interaction between pulsed electric fields and live cells from a molecular point of view in a non-invasive and label-free manner. Raman signatures of live human adipose-derived mesenchymal stem cells exposed or not to pulsed electric fields (8 pulses, 1 000 V/cm, 100 μs, 1 Hz) were acquired at two cellular locations (nucleus and cytoplasm) and two spectral bands (600-1 800 cm(-1) and 2 800-3 100 cm(-1)). Vibrational modes of proteins (phenylalanine and amide I) and lipids were found to be modified by the electropermeabilization process with a statistically significant difference...
January 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28097053/in-vitro-effect-of-direct-current-electrical-stimulation-on-rat-mesenchymal-stem-cells
#16
Sahba Mobini, Liudmila Leppik, Vishnu Thottakkattumana Parameswaran, John Howard Barker
BACKGROUND: Electrical stimulation (ES) has been successfully used to treat bone defects clinically. Recently, both cellular and molecular approaches have demonstrated that ES can change cell behavior such as migration, proliferation and differentiation. METHODS: In the present study we exposed rat bone marrow- (BM-) and adipose tissue- (AT-) derived mesenchymal stem cells (MSCs) to direct current electrical stimulation (DC ES) and assessed temporal changes in osteogenic differentiation...
2017: PeerJ
https://www.readbyqxmd.com/read/28095751/crispr-based-epigenome-editing-of-cytokine-receptors-for-the-promotion-of-cell-survival-and-tissue-deposition-in-inflammatory-environments
#17
Niloofar Farhang, Jonathan M Brunger, Joshua D Stover, Pratiksha I Thakore, Brandon Lawrence, Farshid Guilak, Charles A Gersbach, Lori A Setton, Robert D Bowles
Musculoskeletal diseases have been associated with inflammatory cytokine action, particularly action by TNF-α and IL-1β. These inflammatory cytokines promote apoptosis and senescence of cells in diseased tissue and extracellular matrix breakdown. Stem cell-based therapies are being considered for the treatment of musculoskeletal diseases, but the presence of these inflammatory cytokines will have similar deleterious action on therapeutic cells delivered to these environments. Methods that prevent inflammatory-induced apoptosis and pro-inflammatory signaling, in cell and pathway specific manners are needed...
January 17, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28095649/the-effect-of-mechanical-stress-on-the-proliferation-adipogenic-differentiation-and-gene-expression-of-human-adipose-derived-stem-cells
#18
Nora E Paul, Bernd Denecke, Bong-Sung Kim, Alice Dreser, Jürgen Bernhagen, Norbert Pallua
To allow for a better implementation of external volume expansion to clinical applications for soft tissue regeneration, it is necessary to comprehensively understand the underlying mechanisms. Since human adipose-derived stem cells (hASCs) play a crucial role in soft tissue enlargement, we investigated the impact of cyclic stretch on gene expression, proliferation rate, and adipogenic differentiation of these cells. After cyclic stretching, RNA was extracted and subjected to DNA microarray analysis and RT-qPCR...
January 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28094799/activation-of-wnt-%C3%AE-catenin-signalling-via-gsk3-inhibitors-direct-differentiation-of-human-adipose-stem-cells-into-functional-hepatocytes
#19
Jieqiong Huang, Xinyue Guo, Weihong Li, Haiyan Zhang
The generation of hepatocytes that are derived from human adipose stem cells (hASCs) represents an alternative to human hepatocytes for individualized therapeutic and pharmaceutical applications. However, the mechanisms facilitating hepatocyte differentiation from hASCs are not well understood. Here, we show that upon exposure to glycogen synthase kinase 3 (GSK3) inhibitors alone, the expression of definitive endoderm specific genes GATA4, FOXA2, and SOX17 in hASCs significantly increased in a manner with activation of Wnt/β-catenin signalling...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28093366/strategy-for-constructing-vascularized-adipose-units-in-poly-l-glutamic-acid-hydrogel-porous-scaffold-through-inducing-in-situ-formation-of-ascs-spheroids
#20
Kunxi Zhang, Li Song, Jia Wang, Shifeng Yan, Guifei Li, Lei Cui, Jingbo Yin
: Vascularization is of great importance to adipose tissue regeneration. Here we introduced a paradigm that using scaffold to induce ASC spheroids, so to promote vascularized adipose tissue regeneration. Poly (L-glutamic acid) (PLGA) was activated by EDC, followed by being cross-linked by Adipic dihydrazide (ADH) to form a homogeneous hydrogel. Lyophilization was then carried out to create porous structure. The PLGA hydrogel scaffold possessed a significant swollen hydrophilic network to weaken cell-scaffold adhesion but drive ASCs to aggregate to form spheroids...
January 13, 2017: Acta Biomaterialia
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