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Surinder Kumar, Sanjeev R Saigal, Gulshan Rai Sethi, Sanchit Kumar
CONTEXT: Chlamydophila pneumoniae is a common cause of community-acquired respiratory infections, including pneumonia, bronchitis, and upper respiratory tract infections. Since it is difficult to detect C. pneumoniae in clinical practice, specific etiological diagnosis is established only in a minority of cases. AIMS: To investigate the role of C. pneumoniae in community-acquired lower respiratory tract infections (LRTIs) in children, with the use of serological tests and nested polymerase chain reaction (PCR) analysis...
October 2016: Indian Journal of Pathology & Microbiology
Alyce Taylor-Brown, Nathan L Bachmann, Nicole Borel, Adam Polkinghorne
BACKGROUND: Recent molecular studies have revealed considerably more diversity in the phylum Chlamydiae than was previously thought. Evidence is growing that many of these novel chlamydiae may be important pathogens in humans and animals. A significant barrier to characterising these novel chlamydiae is the requirement for culturing. We recently identified a range of novel uncultured chlamydiae in captive snakes in Switzerland, however, nothing is known about their biology. Using a metagenomics approach, the aim of this study was to characterise the genome of a novel chlamydial taxon from the choana of a captive snake...
2016: BMC Genomics
Priya Maheshwari, Guy D Eslick
BACKGROUND: The possibility of an infectious etiology for Alzheimer's disease (AD) has been repeatedly postulated over the past three decades, with the roles of both viruses and bacteria having been investigated. Chlamydophila (formerly Chlamydia) pneumoniae (Cpn) and spirochetal bacteria have been two of the most frequently implicated bacterial groups in AD pathogenesis. OBJECTIVE: A meta-analysis was performed where data was combined from 25 studies examining the association between AD and spirochetal bacteria or Cpn...
August 18, 2016: Journal of Alzheimer's Disease: JAD
M L Marenzoni, G Morganti, I Moretta, S Crotti, F Agnetti, A Moretti, L Pitzurra, P Casagrande Proietti, P Sechi, B Cenci-Goga, M P Franciosini
Microbiological and parasitological investigation was carried out on a colony of feral pigeons, located in a green area near the main hospital of a Central Italy city. One hundred pigeons were submitted to clinical examination. Cloacal swabs, grouped in pool of 4 samples, were analyzed to detect the presence of Coxiella burnetii, Chlamydia psittaci, Chlamydophila spp. using a biomolecular procedure, while individual cloacal samples were examined for Salmonella spp., Campylobacter spp., and yeasts by means of a specific culture media...
2016: Polish Journal of Veterinary Sciences
Yvonne Pannekoek, Qin Qi-Long, Yu-Zhong Zhang, Arie van der Ende
Many studies have aimed to set up boundaries for the classification and definition of prokaryotic genus and species classification; however, studies that focused on genus-level genomic differences for existing taxonomy systems are limited. Recently, a novel method was described for prokaryotic genus delineation using the percentage of conserved proteins (POCP) between two strains to estimate their evolutionary and phenotypic distance (Qin et al. A proposed genus boundary for the prokaryotes based on genomic insights...
August 2016: Pathogens and Disease
Katarzyna Lewandowska, Jan Kuś
Pneumonia remains one of the main reasons of heath care system utilization. Quick diagnosis and prompt treatment initiation determine favorable outcome. Empirical antibiotic treatment allows to achieve treatment success in most patients. Treatment recommendations are based on big epidemiological trials. Nevertheless, it is sometimes necessary to know the definite etiologic factor of pneumonia. In these cases microbiological diagnostics is useful, i.e. sputum microscopy and culture, blood culture, bronchial lavage culture, bacterial antigen tests in urine, molecular tests...
2016: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
Tara F Carr, Monica Kraft
Chronic bacterial infection is implicated in both the development and severity of asthma. The atypical bacteria Mycoplasma pneumoniae and Chlamydophila pneumoniae have been identified in the airways of asthmatics and correlated with clinical features such as adult onset, exacerbation risks, steroid sensitivity, and symptom control. Asthmatic patients with evidence of bacterial infection may benefit from antibiotic treatment directed towards these atypical organisms. Examination of the airway microbiome may identify microbial communities that confer risk for or protection from severe asthma...
August 2016: Immunology and Allergy Clinics of North America
Elisabetta Caselli, Alessandro Borghi, Martina Maritati, Roberta Gafà, Giovanni Lanza, Dario Di Luca, Annarosa Virgili, Carlo Contini
BACKGROUND: We have previously reported the case of an immunocompetent female patient with a primary cutaneous CD30+ anaplastic large-cell lymphoma (PCALCL) located on her upper right eyelid characterized by the presence of a concurrent active infection by C. pneumoniae and Human herpesvirus 8 (HHV8). This finding suggested for the first time a possible association of C. pneumoniae and/or HHV8 infection, or both together, with PCALCL pathogenesis in non-immunocompromised and HIV-negative subjects...
2016: Infectious Agents and Cancer
Katarzyna Krenke, Ewa Sadowy, Edyta Podsiadły, Waleria Hryniewicz, Urszula Demkow, Marek Kulus
BACKGROUND: An increasing incidence of parapneumonic effusion and pleural empyema (PPE/PE) has been reported in recent studies. As only few data on etiology of PPE/PE in Central Europe have been reported, we undertook a study on the etiology of PPE/PE in children, using both standard culture and molecular techniques. METHODS: This prospective study was conducted between June 2011 and December 2013. Consecutive children with PPE/PE complicating community acquired pneumonia, who required diagnostic/therapeutic thoracentesis were included...
July 2016: Respiratory Medicine
Catia Cillóniz, Antoni Torres, Michael Niederman, Menno van der Eerden, James Chalmers, Tobias Welte, Francesco Blasi
Community-acquired pneumonia (CAP) is associated with high rates of morbidity and mortality worldwide; the annual incidence of CAP among adults in Europe has ranged from 1.5 to 1.7 per 1000 population. Intracellular bacteria are common causes of CAP. However, there is considerable variation in the reported incidence between countries and change over time. The intracellular pathogens that are well established as causes of pneumonia are Legionella pneumophila, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Chlamydophila psittaci, and Coxiella burnetii...
September 2016: Intensive Care Medicine
Ivane Chkhaidze, Dali Zirakishvili, Natalia Shavshvishvili, Neli Barnabishvili
INTRODUCTION: The immune system is known to play a key role in the development of viral-induced wheeze, but the pattern of cytokine response is not clear. The aim of the study was to determine whether plasma cytokine levels during the acute wheezing illness in infants are associated with the subsequent development of persistent recurrent wheezing (PRW). MATERIAL AND METHODS: 43 infants admitted to Iashvili Central Children Hospital, Tbilisi, Georgia, were selected...
2016: Pneumonologia i Alergologia Polska
Gretchen L Parrott, Takeshi Kinjo, Jiro Fujita
Historically, atypical pneumonia was a term used to describe an unusual presentation of pneumonia. Currently, it is used to describe the multitude of symptoms juxtaposing the classic symptoms found in cases of pneumococcal pneumonia. Specifically, atypical pneumonia is a syndrome resulting from a relatively common group of pathogens including Chlamydophila sp., and Mycoplasma pneumoniae. The incidence of M. pneumoniae pneumonia in adults is less than the burden experienced by children. Transmission rates among families indicate children may act as a reservoir and maintain contagiousness over a long period of time ranging from months to years...
2016: Frontiers in Microbiology
Mukhit B Orynbayev, Wendy Beauvais, Abylay R Sansyzbay, Rashida A Rystaeva, Kulyaisan T Sultankulova, Aslan A Kerimbaev, Madina N Kospanova, Richard A Kock
286 serum samples were collected from three sub-populations of saiga in Kazakhstan (Betpakdala, Ustyurt and Volga-Ural) between 2012 and 2014, and were tested for the presence of antibodies to Brucella spp., bluetongue virus, peste des petits ruminants (PPR) virus, Akabane virus, Schmallenberg virus, Chlamydophila, Toxoplasma, Mycobacterium avium subspecies paratuberculosis and Coxiella burnetii (Q Fever). Seropositives to Coxiella burnetii of saiga were detected and the adjusted seroprevalence of Q Fever antibodies was 0...
May 1, 2016: Preventive Veterinary Medicine
Valentina Virginia Ebani, Fabrizio Bertelloni, Paolo Mani
OBJECTIVE: To determine the presence of zoonotic tick-borne bacteria in feral pigeons (Columba livia domestica) from urban areas. METHODS: Spleen samples from 84 feral pigeons, found dead with traumatic injuries in urban areas, were examined by PCR to detect DNA of Anaplasma phagocytophilum, Bartonella spp., Borrelia burgdorferi sensu lato, Coxiella burnetii, Rickettsia spp., and Chlamydophila spp. RESULTS: Twenty (23.8%) pigeons were infected by tick-borne agents, in particular 2 (2...
April 2016: Asian Pacific Journal of Tropical Medicine
Takahiro Sawada, Mitsuhiro Suehiro, Kiho Takaya
No abstract text is available yet for this article.
March 2016: Indian Journal of Dermatology
Eva Spada, Ilaria Canzi, Luciana Baggiani, Roberta Perego, Fabrizio Vitale, Antonella Migliazzo, Daniela Proverbio
Stray cats in the city of Milan, Italy, were tested for Leishmania infantum and other selected infections. Twenty-seven cats (30.0%) were seroreactive by indirect fluorescent antibody test (IFAT), with an antibody titer of 1:40 for 16 (17.7%) cats and 1:80 (cut-off for feline L. infantum infection) for 11 (12.2%) cats. One blood (1.1%) and one popliteal lymph node (1.1%) sample tested positive by real-time polymerase chain reaction; no oculoconjunctival swabs tested positive. Feline immunodeficiency virus, feline leukemia virus, and feline coronavirus (FCoV) seroprevalence determined by enzyme-linked immunosorbent assay was 6...
April 2016: Comparative Immunology, Microbiology and Infectious Diseases
Mariana Herrera, Yudy Alexandra Aguilar, Zulma Vanessa Rueda, Carlos Muskus, Lázaro Agustín Vélez
BACKGROUND: The diagnosis of community-acquired pneumonia (CAP) caused by Legionella pneumophila, Mycoplasma pneumoniae, and Chlamydophila pneumoniae is traditionally based on cultures and serology, which have special requirements, are time-consuming, and offer delayed results that limit their clinical usefulness of these techniques. We sought to develop a multiplex PCR (mPCR) method to diagnosis these bacterial infections in CAP patients and to compare the diagnostic yields obtained from mPCR of nasopharyngeal aspirates (NPAs), nasopharyngeal swabs (NPSs), and induced sputum (IS) with those obtained with specific PCR commercial kits, paired serology, and urinary antigen...
2016: Journal of Negative Results in Biomedicine
Kohji Azumagawa, Shohei Nomura, Yasushi Shigeri, Leslie Sargent Jones, Douglas Kazutoshi Sato, Ichiro Nakashima, Mitsuru Kashiwagi, Takuya Tanabe, Shuichi Shimakawa, Hideto Nakajima, Hiroshi Tamai
The mechanism of post-vaccination acute disseminated encephalomyelitis (ADEM) has been hypothesized as resulting from vaccination-injected antigens cross-reacting with myelin components, however, a precise etiology has been uncertain. In this report, we describe the case of a 6-year-old Japanese boy who had multiphasic disseminated encephalomyelitis (MDEM), and was positive for both anti-myelin oligodendrocyte glycoprotein (MOG) antibodies and Chlamydophila pneumoniae antibodies. After vaccinations that were the second one for measles and rubella, and the booster immunization for Japanese encephalitis, the patient presented with fever, headache, vomiting, and a change in personality...
August 2016: Brain & Development
Mireia Fernandez, Edgar G Manzanilla, Albert Lloret, Marta León, Jean-Christophe Thibault
OBJECTIVES: Our objective was to perform the first multicentric study in Spain to evaluate the prevalence of feline herpesvirus-1 (FHV-1), feline calicivirus (FCV), Chlamydophila felis and Mycoplasma felis in cats with upper respiratory tract disease (URTD), conjunctivitis and/or gingivostomatitis (GS) compared with control cats; and to evaluate risk factors for these clinical conditions. METHODS: Conjunctival and oropharyngeal swabs were collected and a questionnaire regarding signalment, lifestyle, vaccination history and clinical signs was obtained for each cat...
February 26, 2016: Journal of Feline Medicine and Surgery
Markus Raderer, Barbara Kiesewetter, Andrés J M Ferreri
Extranodal marginal zone lymphoma of the mucosa-associated lymphoid tissue (MALT lymphoma) accounts for 7% to 8% of newly diagnosed lymphomas. Because of its association with infectious causes, such as Helicobacter pylori (HP) or Chlamydophila psittaci (CP), and autoimmune diseases, it has become the paradigm of an antigen-driven malignancy. MALT lymphoma usually displays an indolent course, and watch-and-wait strategies are justified initially in a certain percentage of patients. In patients with gastric MALT lymphoma or ocular adnexal MALT lymphoma, antibiotic therapy against HP or CP, respectively, is the first-line management of choice, resulting in lymphoma response rates from 75% to 80% after HP eradication and from 33% to 65% after antibiotic therapy for CP...
March 2016: CA: a Cancer Journal for Clinicians
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