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Elise Sofie Hovingh, Rob Mariman, Luis Solans, Daniëlle Hijdra, Hendrik-Jan Hamstra, Ilse Jongerius, Marjolein van Gent, Frits Mooi, Camille Locht, Elena Pinelli
Whooping cough, caused by Bordetella pertussis, has resurged and presents a global health burden worldwide. B. pertussis strains unable to produce the acellular pertussis vaccine component pertactin (Prn), have been emerging and in some countries represent up to 95% of recent clinical isolates. Knowledge on the effect that Prn deficiency has on infection and immunity to B. pertussis is crucial for the development of new strategies to control this disease. Here, we characterized the effect of Prn production by B...
March 21, 2018: Emerging Microbes & Infections
Jamie Jennings-Gee, Sally Quataert, Tridib Ganguly, Ralph D'Agostino, Rajendar Deora, Purnima Dubey
The reemergence of pertussis or whooping cough in several countries highlights the need for better vaccines. Acellular pertussis vaccines (aPV) contain alum as the adjuvant and elicit Th2 biased immune responses that are less effective in protecting against infection than the reactogenic whole cell pertussis vaccines (wPV), which elicit primarily a Th1/Th17 response. An important goal for the field is to devise aPVs that will induce immune responses similar to wPV. We show that Bordetella Colonization Factor A (BcfA), an outer membrane protein from B...
March 12, 2018: Infection and Immunity
Lucille L F van Beek, Daan D de Gouw, Marc M J Eleveld, Hester H J Bootsma, Marien M I de Jonge, Frits F R Mooi, Aldert A Zomer, Dimitri D A Diavatopoulos
There is a lack of insight into the basic mechanisms by which Bordetella pertussis adapts to the local host environment during infection. We analysed B. pertussis gene expression in the upper and lower airways of mice and compared this to SO4-induced in vitro Bvg-regulated gene transcription. Approximately 30% of all genes were found to be differentially expressed between in vitro vs. in vivo conditions. This included several novel potential vaccine antigens that were exclusively expressed in vivo. Significant differences in expression profile and metabolic pathways were identified between the upper versus the lower airways, suggesting distinct antigenic profiles...
March 8, 2018: Journal of Infectious Diseases
Gaetano Brindicci, Danilo Carboni, Roberto Genga, Eleonora Moschini, Giulia Montorzi, Felicetta Viscogliosi, Giovanni Muratori, Gabriele Ripanti
Pertussis is a contagious, infectious disease that affects mainly children and is caused by Bordetella pertussis. The pertussis vaccine has changed the epidemiology of the disease up to the point when it almost vanished, with a minimum number of cases recorded in Italy (2008) when vaccination coverage was 97%. For the same reason the natural history of the disease was also modified. Indeed, in high-income countries the lack of immunity acquired with the vaccine causes adolescents and adults to become an important source of infection for unvaccinated subjects, the newborn and children who have not completed their primary education...
March 1, 2018: Le Infezioni in Medicina
Runjun Dowarah, Ashok Kumar Verma, Neeta Agarwal, Putan Singh, Bhoj Raj Singh
The present study was aimed to develop an effective probiotic lactic acid bacteria (LAB) from piglet feces and in vitro characterization of probiotic properties. To confirm host-species specificity of probiotics, the efficacy of isolated LAB on growth, nutrient utilization, health and antioxidant status was observed in early weaned piglets. A total of 30 LAB were isolated from feces of five healthy piglets (28d old). All isolates were Gram positive, cocco-bacilli and catalase negative. Out of thirty LAB isolates, twenty were shortlisted on the basis of their tolerance to pH (3...
2018: PloS One
Hitoshi Nakanishi, Masaru Furuya, Takehisa Soma, Yoshiki Hayashiuchi, Ryusaku Yoshiuchi, Makoto Matsubayashi, Hiroyuki Tani, Kazumi Sasai
Objectives Feline gingivostomatitis (FGS) is a painful chronic inflammatory disease of the oral cavity. The purpose of the study was to examine the frequency of detection of certain common feline bacteria and viruses to determine any potential associations with FGS. Methods A multicentre case-control study design was conducted. In total, 72 control cats and 32 cats with FGS were included in the study. Oral swabs were cultured for bacterial identification and a PCR assay was carried out to examine the infection of feline calicivirus (FCV), feline herpesvirus-1 (FHV-1), Chlamydia felis, Mycoplasma felis and Bordetella bronchiseptica...
March 1, 2018: Journal of Feline Medicine and Surgery
Daniel Garrido-Sanz, Javier Manzano, Marta Martín, Miguel Redondo-Nieto, Rafael Rivilla
Polychlorinated biphenyls (PCBs) are widespread persistent pollutants that cause several adverse health effects. Aerobic bioremediation of PCBs involves the activity of either one bacterial species or a microbial consortium. Using multiple species will enhance the range of PCB congeners co-metabolized since different PCB-degrading microorganisms exhibit different substrate specificity. We have isolated a bacterial consortium by successive enrichment culture using biphenyl (analog of PCBs) as the sole carbon and energy source...
2018: Frontiers in Microbiology
Alex-Mikael Barkoff, Jussi Mertsola, Denis Pierard, Tine Dalby, Silje Vermedal Hoegh, Sophie Guillot, Paola Stefanelli, Marjolein van Gent, Guy Berbers, Didrik F Vestrheim, Margrethe Greve-Isdahl, Lena Wehlin, Margaretha Ljungman, Norman K Fry, Kevin Markey, Kari Auranen, Qiushui He
One reason for increased pertussis incidence is the adaptation of Bordetella pertussis to vaccine-induced immunity by modulating its genomic structure. This study, EUpert IV, includes 265 isolates collected from nine European countries during 2012 to 2015 (n=265) and compares the results to previous EUpert I-III studies (1998-2009). The analyses included genotyping, serotyping and pulsed-field gel electrophoresis (PFGE) and multi-locus variable-number tandem repeat analysis (MLVA). Genotyping results showed only small variation among the common virulence genes of B...
February 28, 2018: Journal of Clinical Microbiology
Elodie Lesne, Elian Dupré, Marc F Lensink, Camille Locht, Rudy Antoine, Françoise Jacob-Dubuisson
Bordetella pertussis controls the expression of its virulence regulon through the two-component system BvgAS. BvgS is a prototype for a family of multidomain sensor kinases. In BvgS, helical linkers connect periplasmic Venus flytrap (VFT) perception domains to a cytoplasmic Per-Arnt-Sim (PAS) domain and the PAS domain to the dimerization/histidine phosphotransfer (DHp) domain of the kinase. The two linkers can adopt coiled-coil structures but cannot do so simultaneously. The first linker forms a coiled coil in the kinase mode and the second in the phosphatase mode, with the other linker in both cases showing an increase in dynamic behavior...
February 27, 2018: MBio
Emilia Lönnqvist, Alex-Mikael Barkoff, Jussi Mertsola, Qiushui He
OBJECTIVES: Macrolides, such as azithromycin and erythromycin, are the first line drug for (prophylactic) treatment of pertussis. This study was aimed to screen for macrolide, quinolone, or trimethoprim-sulfamethoxazole resistant strains among Finnish Bordetella pertussis isolates. METHODS: Antimicrobial susceptibility testing was performed on 148 B. pertussis strains isolated during 2006-2017. Isolates were analysed with the allele-specific PCR for detection of macrolide resistance associated mutation A2047G in the 23S rRNA gene...
February 24, 2018: Journal of Global Antimicrobial Resistance
Natosha L Finley
Dissecting how bacterial pathogens escape immune destruction and cause respiratory infections in humans is a work in progress. One tactic employed by microbes is to use bacterial adenylate cyclase toxins (ACTs) to disarm immune cells and disrupt cellular signaling in host cells, which facilitates the infection process. Several clinically significant pathogens, such as Bacillus anthracis and Bordetella pertussis, have ACTs that are stimulated by an activator protein in human cells. Research has shown that these bacterial ACTs have evolved distinct ways of controlling their activities, but our understanding of how the B...
February 27, 2018: PLoS Biology
Timothy J Brickman, Sandra K Armstrong
The classical Bordetella species use amino acids as carbon sources and can catabolize organic acids and tricarboxylic acid cycle intermediates. They are also auxotrophic for nicotinamide adenine dinucleotide (NAD) pathway precursors such as nicotinic acid. Bordetellae have a putative nicotinate catabolism gene locus highly similar to that characterized in Pseudomonas putida KT2440. This study determined the distribution of the nic genes among Bordetella species and analyzed the regulation of this nicotinic acid degradation system...
February 27, 2018: Molecular Microbiology
Federico Martinón-Torres, Ulrich Heininger, Angus Thomson, Carl Heinz Wirsing von König
Pertussis is a highly contagious disease of the respiratory tract which is caused by the bacterium Bordetella pertussis and is most severe in those <1 year of age. A vaccine against pertussis, introduced in the 1950's, led to a significant decrease in incidence of the disease, but recent increases in outbreaks have been attributed to insufficient vaccine uptake, suboptimal protection conferred by vaccines, and waning immunity after immunization. Areas covered: In this review we discuss the major challenges for controlling pertussis, and what we believe the best strategies are to overcome these challenges, focusing on immunization against pertussis in Europe, but with recommendations that are relevant worldwide...
February 27, 2018: Expert Review of Vaccines
Laurence Don Wai Luu, Sophie Octavia, Ling Zhong, Mark Raftery, Vitali Sintchenko, Ruiting Lan
Bordetella pertussis causes whooping cough. The predominant strains in Australia changed to single nucleotide polymorphism (SNP) cluster I (pertussis toxin promoter allele ptxP3/pertactin gene allele prn2) from cluster II (non-ptxP3/non-prn2). Cluster I were mostly responsible for the 2008-2012 Australian epidemic and were found to have higher fitness compared to cluster II using an in vivo mouse competition assay, regardless of host's immunisation status. This study aimed to identify proteomic differences that explain higher fitness in cluster I using iTRAQ, and high-resolution multiple reaction monitoring (MRM-hr)...
February 21, 2018: Proteomics
Evangelia Petridou, Christel Barker Jensen, Athanasios Arvanitidis, Maria Giannaki-Psinaki, Athanasios Michos, Karen Angeliki Krogfelt, Randi Føns Petersen
PURPOSE: To determine the predominant strains of Bordetella pertussis in Greece during 2010-2015. METHODOLOGY: Infants and children (n=1150) (15 days to 14 years) of Greek, Roma and immigrant origin with different vaccination statuses were hospitalized in Athens, Greece with suspected pertussis infection. IS481/IS1001 real-time PCR confirmed Bordetella spp./B. pertussis infection in 300 samples. A subset of samples (n=153) were analysed by multi-locus variable number tandem repeat analysis (MLVA) and (n=25) by sequence-based typing of the toxin promotor region (ptxP) on DNA extracted from clinical specimens...
February 2, 2018: Journal of Medical Microbiology
Winkie Fong, Verlaine Timms, Nadine Holmes, Vitali Sintchenko
Bordetella pertussis, the aetiological agent of whooping cough is routinely diagnosed by polymerase chain reaction (PCR) directed at IS481, an insertion sequence target also found in Bordetella holmesii. Recent reports have suggested that B. holmesii infections can be misdiagnosed as pertussis, which can have a significant impact on public health surveillance. This study investigated the presence of B. holmesii in B. pertussis positive clinical samples, in order to determine the incidence of B. holmesii. Clinical cases of pertussis diagnosed by IS481-specific PCR between October 2008 and March 2016 in New South Wales were included...
February 15, 2018: Pathology
Anaïs Thiriard, Dominique Raze, Camille Locht
The complement cascade participates in protection against bacterial infection and pathogens, including Bordetella pertussis, have developed complement-evading strategies. Here we discuss current knowledge on B. pertussis complement evasion strategies and the role of antibody-dependent complement-mediated killing in protection against B. pertussis infection pointing out important knowledge gaps for further research to improve current pertussis vaccines.
February 14, 2018: Microbes and Infection
Masoud Rahimian, Mahdi HosseiniB
No abstract text is available yet for this article.
February 10, 2018: Respiratory Medicine
Anne-Sophie Debrie, Loïc Coutte, Dominique Raze, Frits Mooi, Frances Alexander, Andrew Gorringe, Nathalie Mielcarek, Camille Locht
Pertussis or whooping cough is currently the most prevalent vaccine-preventable childhood disease despite >85% global vaccination coverage. In recent years incidence has greatly increased in several high-income countries that have switched from the first-generation, whole-cell vaccine to the newer acellular vaccines, calling for improved vaccination strategies with better vaccines. We have developed a live attenuated pertussis vaccine candidate, called BPZE1, which is currently in clinical development. Unlike other pertussis vaccines, BPZE1 has been shown to provide strong protection against infection by the causative agent of pertussis, Bordetella pertussis, in non-human primates...
February 9, 2018: Vaccine
Angela Gentile, María Del Valle Juarez, María Florencia Lucion, Ana Clara Martínez, Viviana Romanin, Soledad Areso, Alicia Mistchenko
INTRODUCTION: Whooping cough continues to be a major cause of morbidity and mortality in infants younger than 1 year. In 2012, Argentina introduced Tdap in pregnancy to prevent infant mortality. The aim of this study is to describe the impact of maternal immunization on the hospitalization and mortality rates of confirmed Bordetella pertussis (Bp) cases by comparing pre- and post-Tdap vaccine recommendation periods. MATERIAL AND METHODS: All PCR-confirmed Bp cases from "R...
February 8, 2018: Vaccine
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