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https://www.readbyqxmd.com/read/28097085/interaction-with-adenylate-cyclase-toxin-from-bordetella-pertussis-affects-the-metal-binding-properties-of-calmodulin
#1
Tzvia I Springer, Corey C Emerson, Christian W Johns, Natosha L Finley
Adenylate cyclase toxin domain (CyaA-ACD) is a calmodulin (CaM)-dependent adenylate cyclase involved in Bordetella pertussis pathogenesis. Calcium (Ca(2+)) and magnesium (Mg(2+)) concentrations impact CaM-dependent CyaA-ACD activation, but the structural mechanisms remain unclear. In this study, NMR, dynamic light scattering, and native PAGE were used to probe Mg(2+)-induced transitions in CaM's conformation in the presence of CyaA-ACD. Mg(2+) binding was localized to sites I and II, while sites III and IV remained Ca(2+) loaded when CaM was bound to CyaA-ACD...
January 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28095485/bordetella-parapertussis-circumvents-neutrophil-extracellular-bactericidal-mechanisms
#2
Juan Gorgojo, Emilia Scharrig, Ricardo M Gómez, Eric T Harvill, Maria Eugenia Rodríguez
B. parapertussis is a whooping cough etiological agent with the ability to evade the immune response induced by pertussis vaccines. We previously demonstrated that in the absence of opsonic antibodies B. parapertussis hampers phagocytosis by neutrophils and macrophages and, when phagocytosed, blocks intracellular killing by interfering with phagolysosomal fusion. But neutrophils can kill and/or immobilize extracellular bacteria through non-phagocytic mechanisms such as degranulation and neutrophil extracellular traps (NETs)...
2017: PloS One
https://www.readbyqxmd.com/read/28080049/modeling-diversity-in-structures-of-bacterial-outer-membrane-lipids
#3
Huilin Ma, Daniel D Cummins, Natalie Brooke Edelstein, Jerry Gomez, Aliza Khan, Masud Dikita Llewellyn, Tara Picudella, Sarah Rose Willsey, Shikha Nangia
Lipopolysaccharides are vital components of the outer membrane of gram-negative bacteria, and they act as extremely strong stimulators of innate immunity in diverse eukaryotic species. The primary immunostimulatory center of the LPS molecule is lipid A-a disaccharide-bound lipophilic domain. Considering the broad diversity in bacterial species, there are variations in the lipid A structure and their immunogenic potency. In this work, we model the lipid A structures of eight commensal or human pathogenic bacterial species: Helicobacter pylori, Porphyromonas gingivalis, Bacteroides fragilis, Bordetella pertussis, Chlamydia trachomatis, Campylobacter jejuni, Neisseria meningitidis, and Salmonella minnesota...
January 12, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28079006/a-new-vaccine-delivery-vehicle-and-adjuvant-candidate-bordetella-pertussis-inactivated-whole-cells-entrapped-in-alginate-microspheres
#4
Naser Mohammadpour Dounighi, Fereshteh Shahcheraghi, Mehdi Razzaghi-Abyaneh, Mojtaba Nofeli, Hossein Zolfagharian
There is no doubt about whole cell pertussis vaccine efficacy, but it is necessary to improve the vaccine quality specially to decreasing its toxicity by obtaining good immunogenicity with low bacteria number content. In this work, under optimum condition inactivated B. pertussis bacteria cells entrapped alginate microparticles were fabricated and in vivo immunogenicity and potency of new microparticle based vaccine evaluated in mice. Microspheres loaded inactive B. pertussis bacterium cells were prepared via an emulsification method and analyzed for morphology, size, polydispersity index, loading efficiency, loading capacity, release profile and in vivo potency...
January 12, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28078081/occurrence-of-pasteurella-multocida-among-pigs-with-respiratory-disease-in-china-between-2011-and-2015
#5
Huisheng Liu, Zhanqin Zhao, Xiaojian Xi, Qiao Xue, Ta Long, Yun Xue
BACKGROUND: Prior to the 1990s, P. multocida capsular serogroup A was the most prevalent in China, followed by serogroups B and D. Thirty years later, serogroup D became the most prevalent, followed by serogroups A and B. However, the P. multocida capsular serogroups currently circulating in China remain unclear. Therefore, the aim of the present study was to provide an update on P. multocida serogroups isolated from diagnostic samples collected from clinically diseased pigs in Central and Eastern China from 2011 to 2015...
2017: Irish Veterinary Journal
https://www.readbyqxmd.com/read/28076445/emerging-bordetella-pertussis-strains-induce-enhanced-signaling-of-human-pattern-recognition-receptors-tlr2-nod2-and-secretion-of-il-10-by-dendritic-cells
#6
Elise S Hovingh, Marjolein van Gent, Hendrik-Jan Hamstra, Marc Demkes, Frits R Mooi, Elena Pinelli
Vaccines against pertussis have been available for more than 60 years. Nonetheless, this highly contagious disease is reemerging even in countries with high vaccination coverage. Genetic changes of Bordetella pertussis over time have been suggested to contribute to the resurgence of pertussis, as these changes may favor escape from vaccine-induced immunity. Nonetheless, studies on the effects of these bacterial changes on the immune response are limited. Here, we characterize innate immune recognition and activation by a collection of genetically diverse B...
2017: PloS One
https://www.readbyqxmd.com/read/28073985/population-based-pertussis-incidence-and-risk-factors-in-infants-less-than-6-months-in-nepal
#7
Michelle M Hughes, Janet A Englund, Jane Kuypers, James M Tielsch, Subarna K Khatry, Laxman Shrestha, Steven C LeClerq, Mark Steinhoff, Joanne Katz
BACKGROUND: Pertussis is estimated to cause 2 percent of childhood deaths globally and is a growing public health problem in developed countries despite high vaccination coverage. Infants are at greatest risk of morbidity and mortality. Maternal vaccination during pregnancy may be effective to prevent pertussis in young infants, but population-based estimates of disease burden in infants are lacking, particularly in low-income countries. The objective of this study was to estimate the incidence of pertussis in infants less than 6 months of age in Sarlahi District, Nepal...
January 10, 2017: Journal of the Pediatric Infectious Diseases Society
https://www.readbyqxmd.com/read/28065204/risk-factors-for-pertussis-in-adults-and-teenagers-in-england
#8
A Wensley, G J Hughes, H Campbell, G Amirthalingam, N Andrews, N Young, L Coole
Pertussis is a vaccine-preventable respiratory infection caused by Bordetella pertussis which can be fatal in infants. Although high vaccine coverage led to prolonged disease control in England, a national outbreak of pertussis in 2011 led to the largest increase in over two decades, including a marked increase in cases aged ⩾15 years. A case-control study in four regions of England was undertaken to investigate risk factors for pertussis in adolescents and adults, specifically employment type and professional and household contact with children...
January 9, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28058755/characteristics-of-bordetella-pertussis-infection-among-infantsand-children-admitted-to-paediatric-intensive-care-units-in-greece-a-multicentre-11-year-study
#9
Maria S Kazantzi, Anargyroula Prezerakou, Serafeia N Kalamitsou, Stavroula Ilia, Panagiotis K Kalabalikis, John Papadatos, Maria M Sdougka, George Briassoulis, Maria N Tsolia
AIM: To describe children with pertussis who required intensive care. METHODS: This is a retrospective analysis of pertussis admissions to all (six) national intensive care units in Greece from 2003 to 2013. RESULTS: A total of 31 children were included, 28 of whom were younger than 12 months old. Cough was the most prominent symptom, being present in 27 of 31 (87%) patients, and on admission, only 7 (22.6%) satisfied the case definition...
January 6, 2017: Journal of Paediatrics and Child Health
https://www.readbyqxmd.com/read/28040392/modifications-of-bordetella-bronchiseptica-core-lipopolysaccharide-influence-immune-response-without-affecting-protective-activity
#10
Federico Sisti, Julieta Fernández, Andrés Cordero, Adriana Casabuono, Alicia Couto, Daniela Hozbor
Bordetella bronchiseptica produces respiratory disease primarily in mammals including humans. Although a considerably amount of research has been generated regarding lipopolysaccharide (LPS) role during infection and stimulating innate and adaptive immune response, mechanisms involved in LPS synthesis are still unknown. In this context we searched in B. bronchiseptica genome for putative glycosyltransferases. We found possible genes codifying for enzymes involved in sugar substitution of the LPS structure. We decided to analyse BB3394 to BB3400 genes, closed to a previously described LPS biosynthetic locus in B...
December 21, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28039663/whooping-cough-in-adults-a-series-of-severe-cases
#11
Katarzyna Zycinska, M Cieplak, M Chmielewska, A Nitsch-Osuch, A Klaczkow, M Hadzik-Blaszczyk, Z Kur, K A Wardyn
Bordetella pertussis is a gram-negative aerobic coccobacillus causing contagious respiratory tract disease called whooping cough. The virulence factors consist of pertussis toxin, filamentous hemagglutinin, fimbriae, lipooligosaccharide, and adenylate cyclase toxin. The disease causes a worldwide threat to public health despite a high vaccination coverage. The course of whooping cough in adults is frequently atypical, causing difficulty in diagnosis. In this report we present five patients hospitalized with Bordetella pertussis infection manifesting atypical and severe symptoms...
December 31, 2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28039302/camp-signaling-of-adenylate-cyclase-toxin-blocks-the-oxidative-burst-of-neutrophils-through-epac-mediated-inhibition-of-phospholipase-c-activity
#12
Ondrej Cerny, Karen E Anderson, Len R Stephens, Phillip T Hawkins, Peter Sebo
The adenylate cyclase toxin-hemolysin (CyaA) plays a key role in immune evasion and virulence of the whooping cough agent Bordetella pertussis. CyaA penetrates the complement receptor 3-expressing phagocytes and ablates their bactericidal capacities by catalyzing unregulated conversion of cytosolic ATP to the key second messenger molecule cAMP. We show that signaling of CyaA-generated cAMP blocks the oxidative burst capacity of neutrophils by two converging mechanisms. One involves cAMP/protein kinase A-mediated activation of the Src homology region 2 domain-containing phosphatase-1 (SHP-1) and limits the activation of MAPK ERK and p38 that are required for assembly of the NADPH oxidase complex...
December 30, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28033240/risk-factors-associated-with-bordetella-pertussis-among-infants-aged-%C3%A2-4-months-in-the-pre-tdap-era-united-states-2002-2005
#13
C Robinette Curtis, Andrew L Baughman, Chas DeBolt, Susan Goodykoontz, Cynthia Kenyon, Barbara Watson, Pamela K Cassiday, Claudia Miller, Lucia C Pawloski, Maria-Lucia C Tondella, Kristine M Bisgard
BACKGROUND: In the United States, infants have the highest reported pertussis incidence and death rates. Improved understanding of infant risk factors is needed to optimize prevention strategies. METHODS: We prospectively enrolled infants ≤4 months of age with incident-confirmed pertussis from 4 sites during 2002-2005 (preceding pertussis-antigen-containing vaccination recommendations for adolescents/adults); each case-patient was age- and site-matched with 2 control subjects...
December 28, 2016: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28030521/immunogenicity-and-safety-of-10-valent-pneumococcal-non-typeable-haemophilus-influenzae-protein-d-conjugate-vaccine-phid-cv-administered-to-children-with-sickle-cell-disease-between-8-weeks-and-2-years-of-age-a-phase-iii-open-controlled-study
#14
Sodiomon B Sirima, Alfred Tiono, Zakaria Gansané, Mohamadou Siribié, Angèle Zongo, Alphonse Ouédraogo, Nancy François, Ana Strezova, Kurt Dobbelaere, Dorota Borys
BACKGROUND: Immunogenicity, safety and reactogenicity of the 10-valent pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) were evaluated in children with sickle cell disease (SCD), who are at increased risk for infections. METHODS: In this phase III, open-label, single center, controlled study in Burkina Faso (NCT01175083), children with SCD (S) or without SCD (NS) were assigned to 6 groups (N=300): children 8-11 weeks of age (<6 months; <6S and <6NS groups) received 3 primary doses and a booster dose of PHiD-CV co-administered with routine childhood vaccines; children 7-11 months of age (7-11S and 7-11NS groups) received 2 primary doses and a booster dose of PHiD-CV; children 12-23 months of age (12-23S and 12-23NS groups) received 2 catch-up doses of PHiD-CV...
December 27, 2016: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28007855/complete-genome-sequences-of-four-bordetella-pertussis-vaccine-reference-strains-from-serum-institute-of-india
#15
Michael R Weigand, Yanhui Peng, Vladimir Loparev, Taccara Johnson, Phalasy Juieng, Sunil Gairola, Rakesh Kumar, Umesh Shaligram, Ramnath Gowrishankar, Hercules Moura, Jon Rees, David M Schieltz, Yulanda Williamson, Adrian Woolfitt, John Barr, M Lucia Tondella, Margaret M Williams
Serum Institute of India is among the world's largest vaccine producers. Here, we report the complete genome sequences for four Bordetella pertussis strains used by Serum Institute of India in the production of whole-cell pertussis vaccines.
December 22, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27993565/acylation-of-the-bordetella-pertussis-cyaa-hemolysin-functional-implications-for-efficient-membrane-insertion-and-pore-formation
#16
Kanungsuk Meetum, Chompounoot Imtong, Gerd Katzenmeier, Chanan Angsuthanasombat
Previously, the ~130-kDa CyaA-hemolysin domain (CyaA-Hly) from Bordetella pertussis co-expressed with CyaC-acyltransferase in Escherichia coli was demonstrated to be palmitoylated at Lys(983) and thus activated its hemolytic activity against target erythrocytes. Here, we report the functional importance of Lys(983)-palmitoylation for membrane insertion and pore formation of CyaA-Hly. Intrinsic fluorescence emissions of both non-acylated CyaA-Hly (NA/CyaA-Hly) and CyaA-Hly were indistinguishable, suggesting no severe conformational change upon acylation at Lys(983)...
December 18, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27977922/proteome-analysis-is-a-valuable-tool-to-monitor-antigen-expression-during-upstream-processing-of-whole-cell-pertussis-vaccines
#17
Bernard Metz, Marieke Hoonakker, Joost P Uittenbogaard, Michel Weyts, Geert P M Mommen, Hugo D Meiring, Wichard Tilstra, Jeroen L A Pennings, Leo A van der Pol, Betsy Kuipers, Arjen Sloots, Jan van den IJssel, Bas van de Waterbeemd, Arno van der Ark
Physicochemical and immunochemical assays were applied to substantiate the relation between upstream processing and the quality of whole-cell pertussis vaccines. Bordetella pertussis bacteria were cultured on a chemically defined medium using a continuous cultivation process in stirred tank reactors to obtain uniform protein expression. Continuous culture favors the consistent production of proteins known as virulence factors. Magnesium sulfate was added during the steady state of the culture in order to diminish the expression of virulence proteins...
December 23, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27974227/evaluation-of-a-commercial-loop-mediated-isothermal-amplification-assay-for-diagnosis-of-bordetella-pertussis-infection
#18
Kazunari Kamachi, Takumi Moriuchi, Yukihiro Hiramatsu, Nao Otsuka, Keigo Shibayama
We evaluated a commercial loop-mediated isothermal amplification (LAMP) assay kit for Bordetella pertussis detection. The LAMP primers were designed to target the ptxP1 allele of the pertussis toxin promoter, but the assay could detect B. pertussis ptxP3 and ptxP8 strains in addition to ptxP1 strains, with high analytical sensitivity.
December 11, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27956730/antimicrobial-susceptibility-and-molecular-detection-of-pertactin-producing-and-pertactin-deficient-bordetella-pertussis
#19
Emily Souder, Jennifer Vodzak, Alan T Evangelista, Sarah S Long
Resurgence of Bordetella pertussis in recent years in the United States has coincided with a dramatic rise in pertactin-deficient strains. Limited data exist on detectability by nucleic acid amplification testing and antimicrobial susceptibility of pertactin-deficient B. pertussis. This study compares 15 pertactin-producing and 15 pertactin-deficient B. pertussis isolates. Pertactin-producing and pertactin-deficient strains were equally detected by nucleic acid amplification testing and were susceptible to antibiotics...
January 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/27933792/oxygen-and-bis-3-5-cyclic-dimeric-guanosine-monophosphate-binding-control-oligomerization-state-equilibria-of-diguanylate-cyclase-containing-globin-coupled-sensors
#20
Justin L Burns, Shannon Rivera, D Douglas Deer, Shawnna C Joynt, David Dvorak, Emily E Weinert
Bacteria sense their environment to alter phenotypes, including biofilm formation, to survive changing conditions. Heme proteins play important roles in sensing the bacterial gaseous environment and controlling the switch between motile and sessile (biofilm) states. Globin coupled sensors (GCS), a family of heme proteins consisting of a globin domain linked by a central domain to an output domain, are often found with diguanylate cyclase output domains that synthesize c-di-GMP, a major regulator of biofilm formation...
December 6, 2016: Biochemistry
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