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https://www.readbyqxmd.com/read/29331588/antagonism-of-cysteinyl-leukotriene-receptor-1-cysltr1-by-montelukast-suppresses-cell-senescence-of-chondrocytes
#1
Wei Song, Yumin Zhang, Jun Wang, Tao Ma, Linjie Hao, Kunzheng Wang
Aging is closely associated with osteoarthritis (OA). Although its underlying mechanisms remain unknown, cellular senescence in chondrocytes has become an important therapeutic target for the treatment of OA. Cysteinyl leukotriene receptors (cysLTRs) mediate the pathobiological function of cysteinyl leukotrienes (cysLTs). However, the roles of cysLTRs in the pathogenesis of OA have not been reported before. In the current study, we found that cysLTR1 but not cysLTR2 is expressed in human primary chondrocytes...
January 10, 2018: Cytokine
https://www.readbyqxmd.com/read/29324332/impaired-plasminogen-binding-in-patients-with-venous-thromboembolism-association-with-protein-carbonylation
#2
Jakub Siudut, Joanna Natorska, Michal Zabczyk, Dorota Zajac, Karolina Seweryn, Maria Rąpała-Kozik, Anetta Undas
INTRODUCTION: Venous thromboembolism (VTE) is associated with hypofibrinolysis. Its mechanisms are poorly understood. We investigated plasminogen-fibrin interaction and its association with fibrinolytic capacity and protein oxidation/carbonylation in VTE patients. MATERIALS AND METHODS: Plasma-purified plasminogen conversion to plasmin and surface plasmon resonance employed for plasminogen-fibrin interactions were individually evaluated in all healthy controls and non-anticoagulated patients following VTE, 10-23months since the event...
January 5, 2018: Thrombosis Research
https://www.readbyqxmd.com/read/29321050/plasminogen-activator-inhibitor-1-a-risk-factor-for-deep-vein-thrombosis-after-total-hip-arthroplasty
#3
Ju Tang, Wei Zhu, Xiaoliang Mei, Zhenxiang Zhang
BACKGROUND: The onset of deep vein thrombosis (DVT) in patients after total hip arthroplasty (THA) may expand or enlarge and subsequently lead to significant mortality. The objective of this study was to investigate potential risk factors for DVT in patients after THA. METHODS: Eligible patients with hip joint diseases who were scheduled for unilateral primary THA at our hospital were prospectively included into this study. The demographic and clinical features, preoperative plasma biomarkers were detailed, recorded, and compared...
January 10, 2018: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29320413/adherence-to-the-mediterranean-diet-and-inflammatory-markers
#4
Antoni Sureda, Maria Del Mar Bibiloni, Alicia Julibert, Cristina Bouzas, Emma Argelich, Isabel Llompart, Antoni Pons, Josep A Tur
The aim was to assess inflammatory markers among adults and adolescents in relation to the adherence to the Mediterranean diet. A random sample (219 males and 379 females) of the Balearic Islands population (12-65 years) was anthropometrically measured and provided a blood sample to determine biomarkers of inflammation. Dietary habits were assessed and the adherence to the Mediterranean dietary pattern calculated. The prevalence of metabolic syndrome increased with age in both sexes. The adherence to the Mediterranean diet in adolescent males was 51...
January 10, 2018: Nutrients
https://www.readbyqxmd.com/read/29314868/bronchiolitis-obliterans-and-pulmonary-fibrosis-after-sulfur-mustard-inhalation-in-rats
#5
Matthew D McGraw, Marilyn M Dysart, Tara B Hendry-Hofer, Paul R Houin, Jacqueline S Rioux, Rhonda B Garlick, Joan E Loader, Russell Smith, Danielle C Paradiso, Wesley W Holmes, Dana R Anderson, Carl W White, Livia A Veress
RATIONALE: Inhalation of powerful chemical agents, such as sulfur mustard (SM), can have debilitating pulmonary consequences, such as bronchiolitis obliterans (BO) and parenchymal fibrosis (PF). The underlying pathogenesis of disorders after SM inhalation is not clearly understood, resulting in a paucity of effective therapies. OBJECTIVE: To evaluate the role of pro-fibrotic pathways involving TGF-β and PDGF in the development of BO and PF after inhalation injury sustained by SM, using a rat model...
January 9, 2018: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/29310804/the-cytoskeletal-network-regulates-expression-of-the-profibrotic-genes-pai-1-and-ctgf-in-vascular-smooth-muscle-cells
#6
Rohan Samarakoon, Paul J Higgins
Vascular smooth muscle cells (VSMCs) are subject to changing hemodynamic stimuli that alter cytoskeletal dynamics, cellular architecture, and structure-associated signal transduction. Tensional stress, force application, and structural perturbations are sensed by VSMCs and impact the physiological as well as pathophysiological responses of the vasculature. Microtubule-targeting drugs provide useful tools to analyze cytoskeletal-associated signaling pathways and their linkages to pathological outcomes. Architecture-based controls on a subset of profibrotic genes commonly expressed in vascular disease are highlighted by their frequent induction in mechanically manipulated cells and with associated changes in cytoskeletal dynamics...
2018: Advances in Pharmacology
https://www.readbyqxmd.com/read/29301516/the-effect-of-dapagliflozin-treatment-on-epicardial-adipose-tissue-volume
#7
Takao Sato, Yoshifusa Aizawa, Sho Yuasa, Shohei Kishi, Koichi Fuse, Satoshi Fujita, Yoshio Ikeda, Hitoshi Kitazawa, Minoru Takahashi, Masahito Sato, Masaaki Okabe
BACKGROUND: Glycosuria produced by sodium-glucose co-transporter-2 (SGLT-2) inhibitors is associated with weight loss. SGLT-2 inhibitors reportedly might reduce the occurrence of cardiovascular events. Epicardial adipose tissue (EAT) is a pathogenic fat depot that may be associated with coronary atherosclerosis. The present study evaluated the relationship between an SGLT-2 inhibitor (dapagliflozin) and EAT volume. METHODS: In 40 diabetes mellitus patients with coronary artery disease (10 women and 30 men; mean age of all 40 patients was 67...
January 4, 2018: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/29287059/microfluidics-a-high-throughput-system-for-the-assessment-of-the-endotheliopathy-of-trauma-and-the-effect-of-timing-of-plasma-administration-on-ameliorating-shock-associated-endothelial-dysfunction
#8
Lawrence N Diebel, Jonathan V Martin, David M Liberati
BACKGROUND: Early resuscitation after trauma-hemorrhagic shock (HS) with plasma rather than crystalloid may ameliorate systemic endothelial cell (EC) injury and dysfunction (endotheliopathy of trauma [EOT]). We postulated that endothelial lined microfluidic networks would be a useful platform to study the endothelial cell activation/injury under flow conditions to mimic HS. We then used the microfluidic system to further characterize the protective effects and optimal timing of plasma infusion on the development of "EOT" in our model...
December 28, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/29280928/thrombin-provokes-degranulation-of-platelet-%C3%AE-granules-leading-to-the-release-of-active-plasminogen-activator-inhibitor-1-pai-1
#9
Benjamin R Huebner, Ernest E Moore, Hunter B Moore, Gregory R Stettler, Geoffrey R Nunns, Peter Lawson, Angela Sauaia, Marguerite Kelher, Anirban Banerjee, Christopher C Silliman
BACKGROUND: The balance of fibrinolytic mediators is crucial to the survival of the critically ill patient, with tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) playing significant roles. While elevated levels of PAI-1 are associated with increased morbidity and mortality, the source of this PAI-1 remains elusive. Platelets contain 90% of circulating plasma PAI-1, however, their ability to release active PAI-1 is controversial. We hypothesize platelets contain active PAI-1 in α-granules capable of immediate degranulation when exposed to high concentrations of thrombin...
December 26, 2017: Shock
https://www.readbyqxmd.com/read/29278662/microrna-335-5p-suppresses-lower-extremity-deep-venous-thrombosis-by-targeted-inhibition-of-pai-1-via-the-tlr4-signaling-pathway
#10
Cui-Xia Bao, Dong-Xia Zhang, Na-Na Wang, Xiang-Kui Zhu, Qi Zhao, Xiao-Lei Sun
OBJECTIVE: This study aims to investigate the effects of microRNA-335-5p (miR-335-5p) on lower-extremity deep vein thrombosis (LEDVT) by targeting PAI-1 through the TLR4 signaling pathway in rat models. METHOD: siRNA, mimic and inhibitor were used for transfection. The miR-335-5p expression was detected by in situ hybridization. CCK-8 assay and flow cytometry were adopted to detect proliferation, cell cycle and apoptosis, respectively. Scratch test and Matrigel-based tube formation assay were used to detect the effect of miR-335-5p on cell migration ability and tube formation ability...
December 26, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29261514/association-of-prothrombotic-adipokine-plasminogen-activator-inhibitor-1-with-tsh-in-metabolic-syndrome-a-case-control-study
#11
Ashok Kumar Ahirwar, Archana Singh, Anju Jain, Kirti Kaim, Shilpa Bhardwaj, Surajeet Kumar Patra, Binita Goswami, M K Bhatnagar, Jayashree Bhattacharjee
Background Metabolic syndrome (MetS) involves a cluster of cardiovascular risk factors, including abnormal lipids, insulin resistance and hypertension. The aim of the present study is to investigate associations between thyroid profile and the pro-thrombotic mediator, plasminogen activator inhibitor-1 (PAI-1), in MetS and identify associated biochemical markers. Materials and methods The present study was a case control study and consisted of 50 diagnosed cases of MetS and 50 healthy volunteers as controls...
December 20, 2017: Hormone Molecular Biology and Clinical Investigation
https://www.readbyqxmd.com/read/29258500/anti-adipogenic-effects-of-the-traditional-herbal-formula-dohongsamul-tang-in-3t3-l1-adipocytes
#12
Eun Hyoung Shim, Hoyoung Lee, Myeong Soo Lee, Sooseong You
BACKGROUND: Blood stasis syndrome (BSS) is a general pattern identification and refers to pathological stagnation of blood circulation, dysfunction of endothelial cells or metabolic disorder in traditional Korean medicine (TKM). Dohongsamul-Tang (DHSMT) is a well-known traditional herbal formula which used for treatment and prevention of BSS by promoting blood circulation in TKM. METHODS: Cytotoxicity of DHSMT was examined by cell counting kit-8 (CCK-8). We also investigated the anti-adipogenesis effect of DHSMT by using Oil Red O staining, intracellular triglyceride assay leptin ELISA and western blot analysis in 3T3-L1 adipocytes...
December 19, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29258201/impact-of-unsaturated-fatty-acids-on-cytokine-driven-endothelial-cell-dysfunction
#13
Simon Trommer, Anja Leimert, Michael Bucher, Julia Schumann
Polyunsaturated fatty acids (PUFA) are reported to exert prophylactic and acute therapeutic effects in diseases linked to endothelial dysfunction. In the present study, the consequences of a PUFA enrichment of endothelial cells (cell line TIME) on cell viability, expression of the cytokines interleukin-6 (IL-6), interleukin-8 (IL-8), granulocyte-macrophage colony-stimulating factor (GM-CSF), and monocyte chemoattractant protein 1 (MCP-1), synthesis of the adhesion molecules intercellular adhesion molecule 1 (ICAM-1) and vascular adhesion molecule 1 (VCAM-1), and production of the coagulation factors plasminogen activator inhibitor-1 (PAI-1), von Willebrand factor (vWF), and tissue factor (TF) was analyzed in parallel...
December 16, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29245935/association-of-mmp-2-rb-and-pai-1-with-decreased-recurrence-free-survival-and-overall-survival-in-bladder-cancer-patients
#14
Owen T M Chan, Hideki Furuya, Ian Pagano, Yoshiko Shimizu, Kanani Hokutan, Lars Dyrskjøt, Jørgen Bjerggaard Jensen, Per-Uno Malmstrom, Ulrika Segersten, Filip Janku, Charles J Rosser
Background: We previously reported an accurate urine-based bladder cancer (BCa)-associated diagnostic signature that can be used to non-invasively detect BCa. In this study, we investigated whether a component of this signature could risk stratify patients with BCa. Methods: Utilizing immunohistochemistry, we investigated angiogenin, MMP-2, p53, RB and PAI-1 expression from 939 patients with BCa. The expression levels were scored by assigning a proportion score and an intensity score to yield a total staining score for each protein...
November 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/29234445/bioactivity-guided-fractionation-of-the-traditional-chinese-medicine-resina-draconis-reveals-loureirin-b-as-a-pai-1-inhibitor
#15
Yu Jiang, Guangping Zhang, Dong Yan, Hong Yang, Zuguang Ye, Tonghui Ma
Thrombotic diseases have become a global burden due to morbidity, mortality, and disability. Traditional Chinese medicine has been proven effective in removing blood stasis and promoting blood circulation, but the exact mechanisms remain unclear. Plasminogen activator inhibitor-1 (PAI-1) is a natural inhibitor of tissue-type and urokinase-type plasminogen activators. In this study, we screened four fractions of Resina Draconis (a traditional Chinese medicine) extract for PAI-1 inhibitory activity. Bioactivity-guided purification and chromogenic substrate-based assay led to the identification of loureirin B as the major PAI-1 inhibitor, with an IC50 value of 26...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29233634/epicardial-adipose-tissue-density-and-volume-are-related-to-subclinical-atherosclerosis-inflammation-and-major-adverse-cardiac-events-in-asymptomatic-subjects
#16
Markus Goeller, Stephan Achenbach, Mohamed Marwan, Mhairi K Doris, Sebastien Cadet, Frederic Commandeur, Xi Chen, Piotr J Slomka, Heidi Gransar, J Jane Cao, Nathan D Wong, Moritz H Albrecht, Alan Rozanski, Balaji K Tamarappoo, Daniel S Berman, Damini Dey
BACKGROUND: We investigated whether epicardial adipose tissue (EAT) volume and density are related to early atherosclerosis, plaque inflammation and major adverse cardiac events (MACE, cardiac death and myocardial infarction) in asymptomatic subjects. METHODS: EAT volume and density were quantified from non-contrast cardiac CT in 456 asymptomatic individuals (age 60.3 ± 8.3; 68% with CCS>0) from the prospective EISNER trial. EAT volume and density were examined in relation to coronary calcium score (CCS), inflammatory biomarkers and MACE...
November 24, 2017: Journal of Cardiovascular Computed Tomography
https://www.readbyqxmd.com/read/29228113/fibroblast-growth-factor-2-and-transforming-growth-factor-beta1-oppositely-regulate-mir-221-that-targets-thrombospondin-1-in-bovine-luteal-endothelial-cells
#17
Svetlana Farberov, Rina Meidan
Thrombospondin-1 (THBS1) is an important mediator of corpus luteum (CL) regression. Highly induced during luteolysis, it acts as a natural anti-angiogenic, proapoptotic compound. THBS1 expression is regulated in bovine luteal endothelial cells (LECs) by fibroblast growth factor-2 (FGF2) and transforming growth factor-beta1 (TGFB1) acting in an opposite manner. In this study we sought to identify specific microRNAs (miRNAs) targeting THBS1 and investigate their possible involvement in FGF2 and TGFB1-mediated THBS1 expression...
December 6, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29227673/crystal-structure-of-cleaved-serp-1-a-myxomavirus-derived-immune-modulating-serpin-structural-design-of-serpin-reactive-center-loop-rcl-peptides-with-improved-therapeutic-function
#18
Brian P Mahon, Sriram Ambadapadi, Jordan R Yaron, Carrie L Lomelino, Melissa A Pinard, Shahar Keinan, Igor Kurnikov, Colin Macaulay, Liqiang Zhang, Westley Reeves, Grant McFadden, Scott Tibbetts, Robert McKenna, Alexandra Lucas
The Myxomavirus-derived protein Serp-1 has potent anti-inflammatory activity in models of vasculitis, lupus, viral sepsis, and transplant. Serp-1 has also been tested successfully in a Phase IIa clinical trial in unstable angina, representing a 'first-in-class' therapeutic. Recently, peptides derived from the reactive center loop (RCL) have been developed as stand-alone therapeutics for reducing vasculitis and improving survival in MHV68-infected mice. However, both Serp-1 and the RCL peptides lose activity in MHV68-infected mice after antibiotic suppression of intestinal microbiota...
December 11, 2017: Biochemistry
https://www.readbyqxmd.com/read/29221711/sitosterol-prevents-obesity-related-chronic-inflammation
#19
Makoto Kurano, Koji Hasegawa, Motoei Kunimi, Masumi Hara, Yutaka Yatomi, Tamio Teramoto, Kazuhisa Tsukamoto
The physiological roles of phytosterols in chronic inflammation, which are believed to be involved in the underlying mechanisms for metabolic diseases, have yet to be elucidated. Therefore, in the present study, we aimed to elucidate the physiological roles of phytosterols in both clinical studies and animal experiments. We observed the existence of rather specific negative correlations between the serum sitosterol level and the serum IL-6 and the TNF-α levels in both diabetic subjects (n=46) and non-diabetic subjects (n=178)...
December 6, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29218943/-effect-of-germacrone-in-alleviating-huvecs-damaged-by-h2o2-induced-oxidative-stress
#20
Qiong-Fang Chen, Gang Wang, Li-Qing Tang, Xian-Wen Yu, Zhao-Fei Li, Xiu-Fen Yang
This study focuses on the protective effect of germacrone on human umbilical vein endothelial cells(HUVECs) damaged by H2O2-induced oxidative stress and its possible mechanisms. The oxidative damage model was established by using 500 μmol•L⁻¹ H2O2 to treat HUVECs for 3 hours, and then protected with different concentrations of germacrone for 24 hours. The effect of germacrone on cell viability of HUVECs damaged by H2O2 was detected by MTT. The contents of PGI2, TXB2, ET-1, t-PA, PAI-1, TNF-α and IL-6 were detected by ELISA...
September 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
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