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https://www.readbyqxmd.com/read/28088270/association-of-plasminogen-activator-inhibitor-1-and-vitamin-d-receptor-expression-with-the-risk-of-keloid-disease-in-a-chinese-population
#1
Zhen-Hua Gong, Jian-Feng Ji, Jun Yang, Tie Xiang, Chang-Kai Zhou, Xuan-Liang Pan, Jian Yao
Keloid disease (KD) is a benign fibroproliferative scarring condition of unknown etiopathogenesis. Plasminogen activator inhibitor-1 (PAI-1) and vitamin D receptor (VDR) have been shown to play important roles in the progression of tissue fibrosis; therefore, both these genes are potential susceptibility genes for KD. We aimed to determine whether the gene expression levels of PAI-1 and VDR are altered in Chinese KD patients. We measured the expression of PAI and VDR in human peripheral blood lymphocytes in 236 patients with keloid and 219 age- and sex-matched healthy controls by quantitative real-time polymerase chain reaction...
January 2017: Kaohsiung Journal of Medical Sciences
https://www.readbyqxmd.com/read/28081568/non-lethal-endotoxin-injection-a-rat-model-of-hypercoagulability
#2
Marjory B Brooks, James R Turk, Abraham Guerrero, Padma K Narayanan, John P Nolan, Elizabeth G Besteman, Dennis W Wilson, Roberta A Thomas, Cindy E Fishman, Karol L Thompson, Heidrun Ellinger-Ziegelbauer, Jennifer B Pierson, April Paulman, Alan Y Chiang, Albert E Schultze
Systemic inflammation co-activates coagulation, which unchecked culminates in a lethal syndrome of multi-organ microvascular thrombosis known as disseminated intravascular coagulation (DIC). We studied an endotoxin-induced inflammatory state in rats to identify biomarkers of hemostatic imbalance favoring hypercoagulability. Intraperitoneal injection of LPS at 15 mg/kg body weight resulted in peripheral leukopenia and widespread neutrophilic sequestration characteristic of an acute systemic inflammatory response...
2017: PloS One
https://www.readbyqxmd.com/read/28081538/testing-breast-cancer-serum-biomarkers-for-early-detection-and-prognosis-in-pre-diagnosis-samples
#3
Anna Kazarian, Oleg Blyuss, Gergana Metodieva, Aleksandra Gentry-Maharaj, Andy Ryan, Elena M Kiseleva, Olga M Prytomanova, Ian J Jacobs, Martin Widschwendter, Usha Menon, John F Timms
BACKGROUND: Breast cancer is a leading cause of morbidity and mortality worldwide. Although mammography screening is available, there is an ongoing interest in improved early detection and prognosis. Herein, we have analysed a combination of serological biomarkers in a case-control cohort of sera taken before diagnosis. METHODS: This nested case-control study within the UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS) used serum samples from 239 women who subsequently developed breast cancer and 239 matched cancer-free controls...
January 12, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28081351/the-effect-of-methylprednisolone-treatment-on-fibrinolysis-thecoagulation-system-and-blood-loss-in-cardiac-surgery
#4
Leila Ebrahimi, Maryam Kheirandish, Mahnoosh Foroughi
BACKGROUND/AIM: The purpose of this study was to examine steroid pretreatment in order to decrease postoperative coagulopathy disorders and bleeding. MATERIALS AND METHODS: In this randomized double-blinded study, the efficacy of low versus high doses of methylprednisolone on the coagulation system and postoperative bleeding was compared in patients who were undergoing cardiac surgery with cardiopulmonary bypass (CPB). The platelet response to agonists, D-dimer concentration, tissue plasminogen activator (tPA), plasminogen activator inhibitor (PAI-1) antigens, and platelet receptors CD42b, CD62P, and CD41a were evaluated...
December 20, 2016: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28065908/ticagrelor-but-not-clopidogrel-active-metabolite-displays-antithrombotic-properties-in-the-left-atrial-endocardium
#5
Martin F Reiner, Alexander Breitenstein, Erik W Holy, Martina Glanzmann, Heidi Amstalden, Simon F Stämpfli, Nicole R Bonetti, Volkmar Falk, Stephan Keller, Gianluigi Savarese, Stefano Benussi, Francesco Maisano, Thomas F Lüscher, Jürg H Beer, Jan Steffel, Giovanni G Camici
AIMS: Oral anticoagulation is considered standard therapy for stroke prevention in atrial fibrillation (AF). Endocardial activation triggers expression of pro-thrombotic mediators including tissue factor (TF) and plasminogen activator inhibitor-1 (PAI-1), and contributes to thrombus formation in the left atrial appendage (LAA) of AF patients. Recently, pleiotropic effects of specific P2Y12 receptor antagonists were demonstrated; however, whether these drugs possess antithrombotic effects on LAA endocardial cells currently remains unknown...
January 8, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28064471/vasohibin-2-is-required-for-epithelial-mesenchymal-transition-of-ovarian-cancer-cells-by-modulating-tgf-%C3%AE-signaling
#6
Rie Norita, Yasuhiro Suzuki, Yutaka Furutani, Kazuki Takahashi, Yasuhiro Yoshimatsu, Katarzyna A Podyma-Inoue, Tetsuro Watabe, Yasufumi Sato
Vasohibin-2 (VASH2) is a homologue of VASH1, an endothelium-derived angiogenesis inhibitor. VASH2 is mainly expressed in cancer cells, and has been implicated in the progression of cancer by inducing angiogenesis and tumor growth. While VASH2 has been recently reported to be involved in epithelial-mesenchymal transition (EMT), its precise roles are obscure. The aim of the present study was to clarify the role of VASH2 in the EMT of cancer cells in relation to TGF-β signaling, which is a major stimulator of the EMT...
January 8, 2017: Cancer Science
https://www.readbyqxmd.com/read/28061787/plasma-markers-of-inflammation-and-hemostatic-and-endothelial-activity-in-naturally-overweight-and-obese-dogs
#7
R Barić Rafaj, J Kuleš, A Marinculić, A Tvarijonaviciute, J Ceron, Ž Mihaljević, A Tumpa, V Mrljak
BACKGROUND: Obesity is one of the most prevalent health problems in the canine population. While haemostatic parameters and markers of endothelial function have been evaluated in various disease conditions in dogs, there are no studies of these markers in canine obesity. This study was designed to evaluate the effect of naturally gained weight excess and obesity on inflammatory, hemostatic and endothelial biomarkers in dogs. A total of 37 overweight and obese dogs were compared with 28 normal weight dogs...
January 6, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28052725/paracrine-activity-from-adipose-derived-stem-cells-on-in-vitro-wound-healing-in-human-tympanic-membrane-keratinocytes
#8
Huan Ting Ong, Sharon Redmond, Robert Marano, Marcus Atlas, Magnus von Unge, Peder Aabel, Rodney J Dilley
Stem cell therapies for tympanic membrane repair have shown initial experimental success using mesenchymal stem cells in rat models to promote healing, however the mechanisms providing this benefit are not known. We investigated in vitro the paracrine effects of human adipose-derived stem cells (ADSC) on wound healing mechanisms for human tympanic membrane-derived keratinocytes (hTM) and immortalised human keratinocytes (HaCat). ADSC conditioned media (CMADSC) were assessed for paracrine activity on keratinocyte proliferation and migration, with hypoxic conditions for ADSC culture used to generate contrasting effects on cytokine gene expression...
January 4, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28043356/anethole-improves-the-in%C3%A2-vitro-development-of-isolated-caprine-secondary-follicles
#9
N A R Sá, V R Araújo, H H V Correia, A C A Ferreira, D D Guerreiro, A M Sampaio, E Escobar, F W Santos, A A Moura, C H Lôbo, V M Ceccatto, C C Campello, A P R Rodrigues, J H Leal-Cardoso, J R Figueiredo
The aim of this study was to investigate the effect of three concentrations of anethole (30, 300, and 2000 μg/mL) on survival, antrum formation, follicular diameter, and oocyte maturation in the caprine species. The study also evaluated the effects of anethole on transcripts of ICAM-1, CAV-1, TIMP-2, and PAI-1 genes and levels of reactive oxygen species (ROS) in isolated goat preantral ovarian follicles before and after in vitro culture for 18 days. Preantral follicles were isolated from goat ovaries and individually cultured in alpha minimum essential medium modified (α-MEM(+)), defined as the control treatment, α-MEM(+) supplemented with ascorbic acid at a concentration of 100 μg/mL (AA), or α-MEM(+) supplemented with three different concentrations of anethole (30, 300, 2000 μg/mL) for a period of 18 days...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043040/rho-kinase-inhibitor-treatment-prevents-pulmonary-inflammation-and-coagulation-in-lipopolysaccharide-induced-lung-injury
#10
Renyu Ding, Dongmei Zhao, Xiaoxia Li, Baoyan Liu, Xiaochun Ma
INTRODUCTION: In the pathogenesis of sepsis-induced acute lung injury (ALI), the crosstalk between inflammation and coagulation plays a pivotal role. The aim of this study was to investigate the role of Rho kinase (ROCK) inhibitor in alleviating pulmonary inflammation and coagulation in lipopolysaccharide (LPS)-induced acute lung injury (ALI) models. METHODS: In the in vivo study, mice were randomized to four different groups: Control, Y-27632 (Y), LPS, and LPS+Y-27632 (LPS+Y)...
December 24, 2016: Thrombosis Research
https://www.readbyqxmd.com/read/28028973/the-impact-of-0-9-nacl-on-mesothelial-cells-after-intraperitoneal-lavage-during-surgical-procedures
#11
Jarosław Cwaliński, Andrzej Bręborowicz, Alicja Połubińska
BACKGROUND: Normal saline gained wide popularity in abdominal surgery as a basic compound used in intraoperative drainage of the peritoneal cavity. However, recent studies have revealed that saline solution is not quite biocompatible with the intraperitoneal enviroment and may promote peritoneal adhesions. OBJECTIVES: The aim of the study was to evaluate the function and viability of human mesothelial cells cultured in vitro in 0.9% NaCl solution from intraperitoneal lavage carried out during laparoscopic cholecytectomies...
November 2016: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/28018998/fibrinolysis-in-patients-with-a-mild-to-moderate-bleeding-tendency-of-unknown-cause
#12
Johanna Gebhart, Sylvia Kepa, Stefanie Hofer, Silvia Koder, Alexandra Kaider, Alisa S Wolberg, Helmuth Haslacher, Peter Quehenberger, Ernst Eigenbauer, Simon Panzer, Christine Mannhalter, Ingrid Pabinger
In more than 50% of patients with a mild-to-moderate bleeding tendency, no underlying cause can be identified (bleeding of unknown cause, BUC). Data on parameters of fibrinolysis in BUC are scarce in the literature and reveal discrepant results. It was the aim of this study to investigate increased fibrinolysis as a possible mechanism of BUC. We included 270 patients (227 females, median age 44 years, 25-75(th) percentile 32-58) with BUC and 98 healthy controls (65 females, median age 47 years, 25-75(th)percentile 39-55)...
December 26, 2016: Annals of Hematology
https://www.readbyqxmd.com/read/28004797/acute-transcriptional-up-regulation-specific-to-osteoblasts-osteoclasts-in-medaka-fish-immediately-after-exposure-to-microgravity
#13
Masahiro Chatani, Hiroya Morimoto, Kazuhiro Takeyama, Akiko Mantoku, Naoki Tanigawa, Koji Kubota, Hiromi Suzuki, Satoko Uchida, Fumiaki Tanigaki, Masaki Shirakawa, Oleg Gusev, Vladimir Sychev, Yoshiro Takano, Takehiko Itoh, Akira Kudo
Bone loss is a serious problem in spaceflight; however, the initial action of microgravity has not been identified. To examine this action, we performed live-imaging of animals during a space mission followed by transcriptome analysis using medaka transgenic lines expressing osteoblast and osteoclast-specific promoter-driven GFP and DsRed. In live-imaging for osteoblasts, the intensity of osterix- or osteocalcin-DsRed fluorescence in pharyngeal bones was significantly enhanced 1 day after launch; and this enhancement continued for 8 or 5 days...
December 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28000463/obesity-endothelial-function-and-inflammation-the-effects-of-weight-loss-after-bariatric-surgery
#14
Fátima Illán Gómez, Manuel Gonzálvez Ortega, Aurora Aragón Alonso, Sabel Orea Soler, M ª Soledad Alcaraz Tafalla, Matías Pérez Paredes, M ª Luisa Lozano Almela
OBJECTIVE: Obesity is associated with a high risk for atherosclerotic cardiovascular disease. There is a causal association between obesity, inflammation, insulin resistance (IR) and endothelial dysfunction. The aim of this study was to evaluate changes in IR, proinflammatory state and markers of endothelial dysfunction in morbidly obese patients after weight loss following bariatric surgery. METHODS: In this study, we measured the levels of soluble intracellular adhesion molecule-1 (sICAM1), plasminogen activator inhibitor 1 (PAI-1), high-sensitivity C-reactive protein (hs-CRP), and interleukin-6 (IL-6) in 79 morbidly obese patients at baseline and 3, 6 and 12 months after gastric bypass...
November 29, 2016: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/28000367/cell-adhesive-peptides-functionalized-on-cocr-alloy-stimulate-endothelialization-and-prevent-thrombogenesis-and-restenosis
#15
Maria Isabel Castellanos, Jordi Guillem-Marti, Carlos Mas-Moruno, Maribel Díaz-Ricart, Ginés Escolar, Maria Pau Ginebra, Francisco Javier Gil, Marta Pegueroles, Jose María Manero
Immobilization of bioactive peptide sequences on CoCr surfaces is an effective route to improve endothelialization, which is of great interest for cardiovascular stents. In this work, we explored the effect of physical and covalent immoblization of RGDS, YIGSR and their equimolar combination peptides on endothelial cells (EC) and smooth muscle cell (SMC) adhesion and on thrombogenicity. We extensively investigated using RT-qPCR, the expression by ECs cultured on functionalised CoCr surfaces of different genes...
December 21, 2016: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/27994710/serum-from-varicose-patients-induces-senescence-related-dysfunction-of-vascular-endothelium-generating-local-and-systemic-proinflammatory-conditions
#16
Justyna Mikuła-Pietrasik, Paweł Uruski, Krzysztof Aniukiewicz, Patrycja Sosińska, Zbigniew Krasiński, Andrzej Tykarski, Krzysztof Książek
Although the role of endothelium in varicose vein development is indisputable, the effect of the pathology on biological properties of endothelial cells remains unclear. Here we examined if the presence of varicose veins affects senescence of endothelial cells (HUVECs) and, if so, what will be the local and systemic outcome of this effect. Experiments showed that HUVECs subjected to serum from varicose patients display improved proliferation, increased expression of senescence marker, SA-β-Gal, and increased generation of reactive oxygen species (ROS), as compared with serum from healthy donors...
2016: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/27994515/dose-dependent-effects-of-strontium-ranelate-on-ovariectomy-rat-bone-marrow-mesenchymal-stem-cells-and-human-umbilical-vein-endothelial-cells
#17
Xiaojing Guo, Silong Wei, Mengmeng Lu, Zhengwei Shao, Jiayu Lu, Lunguo Xia, Kaili Lin, Derong Zou
In clinic, strontium ranelate (SrR) is a useful drug to treat osteoporosis by orally taken method, but some side effect appeared in recent years. The aim of this study is to evaluate the effectiveness and safety of SrR on cells by direct application, to study the possibility of local application of this drug. Qualitative ALP staining, quantitative ALP activity assay, alizarin red staining, realtime PCR and westernblot assay were used to evaluate the osteogenesis ability of SrR under normal or osteogenic induction environment of ovariectomy bone marrow mesenchymal stem cells (OVX-BMSCs)...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27992087/urokinase-type-plasminogen-activator-modulates-mammalian-circadian-clock-phase-regulation-in-tissue-type-plasminogen-activator-knockout-mice
#18
Joanna M Cooper, Ashutosh Rastogi, Jessica A Krizo, Eric M Mintz, Rebecca A Prosser
Glutamate phase shifts the circadian clock in the mammalian suprachiasmatic nucleus (SCN) by activating NMDA receptors. Tissue-type plasminogen activator (tPA) gates phase shifts by activating plasmin to generate m(ature)BDNF, which binds TrkB receptors allowing clock phase shifts. Here we investigate phase shifting in tPA knockout (tPA(-/-) ; B6.129S2-Plat(tm1Mlg) /J) mice, and identify urokinase-type plasminogen activator (uPA) as an additional circadian clock regulator. Behavioral activity rhythms in tPA(-/-) mice entrain to a light-dark (LD) cycle and phase shift in response to nocturnal light pulses with no apparent loss in sensitivity...
December 19, 2016: European Journal of Neuroscience
https://www.readbyqxmd.com/read/27975104/prognostic-significance-of-tpa-pai-1-complex-in-patients-with-heart-failure-and-preserved-ejection-fraction
#19
Max-Paul Winter, Marcus E Kleber, Lorenz Koller, Patrick Sulzgruber, Hubert Scharnagl, Graciela Delgado, Georg Goliasch, Winfried März, Alexander Niessner
Heart failure with preserved ejection fraction (HFpEF) represents a major epidemic, clinical and public health problem with rising patient numbers every year. Traditional markers for heart failure have been shown to be of limited sensitivity in patients with HFpEF, as those do not reflect pathophysiology of the disease properly. Dysregulation of haemostasis is thought to be central for the initiation and progression of HFpEF. For this reason, we aimed to assess markers of fibrinolytic activity as potential biomarkers for risk assessment in patients with HFpEF...
December 15, 2016: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/27975098/endotoxaemia-augmented-murine-venous-thrombosis-is-dependent-on-tlr-4-and-icam-1-and-potentiated-by-neutropenia
#20
Andrea T Obi, Elizabeth Andraska, Yogendra Kanthi, Chase W Kessinger, Megan Elfline, Cathy Luke, Teruna J Siahaan, Farouc A Jaffer, Thomas W Wakefield, Peter K Henke
Venous thromboembolism is a major cause of death during and immediately post-sepsis. Venous thrombosis (VT) is mediated by cell adhesion molecules and leukocytes, including neutrophil extracellular traps (NETs). Sepsis, or experimentally, endotoxaemia, shares similar characteristics and is modulated via toll like receptor 4 (TLR4). This study was undertaken to determine if endotoxaemia potentiates early stasis thrombogenesis, and secondarily to determine the role of VT TLR4, ICAM-1 and neutrophils (PMNs). Wild-type (WT), ICAM-1(-/-) and TLR4(-/-) mice underwent treatment with saline or LPS (10 mg/kg i...
December 15, 2016: Thrombosis and Haemostasis
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