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https://www.readbyqxmd.com/read/29775893/puerarin-prevents-vascular-endothelial-injury-through-suppression-of-nf-%C3%AE%C2%BAb-activation-in-lps-challenged-human-umbilical-vein-endothelial-cells
#1
Hua-Fei Deng, Sha Wang, Lian Li, Qin Zhou, Wan-Bei Guo, Xiao-Li Wang, Mei-Dong Liu, Ke Liu, Xian-Zhong Xiao
OBJECTIVE: In the present study, we aimed to explore the effects of puerarin on vascular endothelial cell injury induced by lipopolysaccharide (LPS) and its underlying mechanisms. METHODS: The cell viability and morphological changes were assessed using the cell counting kit-8 (CCK-8) assay and 4´,6-diamidino-2-phenylindole (DAPI) staining, respectively. The levels of tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), monocyte/macrophage chemotactic protein-1 (MCP-1), IL-8, intercellular cell adhesion molecule-1 (ICAM-1), thrombomodulin (TM) and plasminogen activator inhibitor-1 (PAI-1) in cell culture supernatant were determined by the enzyme-linked immunosorbent assay (ELISA)...
May 15, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29775223/curcumin-down-regulates-il-17a-mediated-p53-fibrinolytic-system-in-bleomycin-induced-acute-lung-injury-in-vivo
#2
Mahesh M Gouda, Yashodhar P Bhandary
Bleomycin (BLM) induced cellular damage causes inflammation in the alveolar compartment and impairment of fibrinolytic system leads to alveolar epithelial cell apoptosis. Here, we describe novel inflammatory pathway associated with p53-fibrinolytic system and apoptosis of alveolar epithelial cells and pharmacological efficiency of curcumin against this action. In the present study we used C57BL/6 mice. The specific dose and time interval of curcumin were analyzed to assess the intervention. Experiments were designed to investigate the IL-17A mediated modulation in the alveolar epithelial cell apoptosis and injury...
May 18, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29769125/observational-study-of-coagulation-activation-in-early-breast-cancer-development-of-a-prognostic-model-based-on-data-from-the-real-world-setting
#3
Chiara Mandoj, Laura Pizzuti, Domenico Sergi, Isabella Sperduti, Marco Mazzotta, Luigi Di Lauro, Antonella Amodio, Silvia Carpano, Anna Di Benedetto, Claudio Botti, Francesca Ferranti, Anna Antenucci, Maria Gabriella D'Alessandro, Paolo Marchetti, Silverio Tomao, Giuseppe Sanguineti, Antonio Giordano, Marcello Maugeri-Saccà, Gennaro Ciliberto, Laura Conti, Patrizia Vici, Maddalena Barba
BACKGROUND: Cancer and coagulation activation are tightly related. The extent to which factors related to both these pathologic conditions concur to patient prognosis intensely animates the inherent research areas. The study herein presented aimed to the development of a tool for the assessment and stratification of risk of death and disease recurrence in early breast cancer. METHODS: Between 2008 and 2010, two hundreds thirty-five (N: 235) patients diagnosed with stage I-IIA breast cancer were included...
May 16, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29768059/pentabromopseudilin-a-myosin-v-inhibitor-suppresses-tgf-%C3%AE-activity-by-recruiting-the-type-ii-tgf-%C3%AE-receptor-to-lysosomal-degradation
#4
Wang Shih-Wei, Chung Chih-Ling, Yu-Chen Kao, René Martin, Hans-Joachim Knölker, Meng-Shin Shiao, Chun-Lin Chen
Pentabromopseudilin (PBrP) is a marine antibiotic isolated from the marine bacteria Pseudomonas bromoutilis and Alteromonas luteoviolaceus. PBrP exhibits antimicrobial, anti-tumour, and phytotoxic activities. In mammalian cells, PBrP is known to act as a reversible and allosteric inhibitor of myosin Va (MyoVa). In this study, we report that PBrP is a potent inhibitor of transforming growth factor-β (TGF-β) activity. PBrP inhibits TGF-β-stimulated Smad2/3 phosphorylation, plasminogen activator inhibitor-1 (PAI-1) protein production and blocks TGF-β-induced epithelial-mesenchymal transition in epithelial cells...
December 2018: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/29763839/salivary-fingerprint-of-simple-obesity
#5
Anna Lehmann-Kalata, Izabela Miechowicz, Katarzyna Korybalska, Ewelina Swora-Cwynar, Natasza Czepulis, Joanna Łuczak, Zofia Orzechowska, Marian Grzymisławski, Anna Surdacka, Janusz Witowski
BACKGROUND: The nature of a link between poor oral health and obesity is not fully understood. It is also unclear if saliva contributes to it and whether the properties of saliva change as a result of an increase in body mass or rather as a consequence of obesity-associated comorbidities. This pilot study was undertaken in an attempt to determine if salivary biomarkers can identify obesity per se. METHODS: Whole mixed saliva was analysed for 16 soluble parameters covering 4 categories (inflammation, oxidative stress, endothelial dysfunction, adipokines)...
May 11, 2018: Cytokine
https://www.readbyqxmd.com/read/29761522/thrombotic-characteristics-of-extracellular-vesicles-derived-from-prostate-cancer-cells
#6
Hassan A Al Saleh, Sandor Haas-Neill, Ali Al-Hashimi, Anil Kapoor, Bobby Shayegan, Richard C Austin, Khalid Al-Nedawi
BACKGROUND: Prostate cancer (PC) patients in advanced stages of the disease have high risk of blood coagulation complications. The procoagulant molecule Tissue factor (TF), and the fibrinolysis inhibitor plasminogen activator inhibitor-1 PAI-1 play important role in this complication. Extracellular vesicles (EV) shed from cancer cells may contribute to the regulation of TF and PAI-1. The procoagulant activity of EV can be associated with the oncogenic and metastatic characteristics of their cells...
May 15, 2018: Prostate
https://www.readbyqxmd.com/read/29758118/hemostasis-biomarkers-in-multiple-sclerosis
#7
Nicole Ziliotto, Francesco Bernardi, Dejan Jakimovski, Marcello Baroni, Giovanna Marchetti, Niels Bergsland, Deepa P Ramasamy, Bianca Weinstock-Guttman, Ferdinand Schweser, Paolo Zamboni, Murali Ramanathan, Robert Zivadinov
OBJECTIVE: To investigate the plasma levels of hemostasis components in multiple sclerosis (MS) and their association with clinical and MRI outcomes. METHODS: We studied 138 MS patients (85 with relapsing-remitting, RR-MS and 53 with progressive, P-MS) with mean age of 54 years; 72.5% female; median EDSS 3.5; mean disease duration 21 years) and 42 age- and sex-matched healthy individuals (HI). All subjects were examined with 3T MRI and clinical examinations. Plasma levels of hemostasis factors (procoagulant, factor XII, FXII) and inhibitors (tissue factor pathway inhibitor (TFPI), thrombomodulin (TM), heparin cofactor II (HCII), disintegrin-like and metalloprotease with thrombospondin type 1 motif 13 (ADAMTS13), and plasminogen activator inhibitor 1 (PAI-1) were evaluated by magnetic Luminex assays and ELISA...
May 14, 2018: European Journal of Neurology: the Official Journal of the European Federation of Neurological Societies
https://www.readbyqxmd.com/read/29739057/cell-line-controls-for-the-genotyping-of-a-spectrum-of-human-single-nucleotide-polymorphisms-in-the-clinical-laboratory
#8
Christine Kimbacher, Christian Paar, Andrea Freystetter, Joerg Berg
BACKGROUND: Genotyping for clinically important single nucleotide polymorphisms (SNPs) is performed by many clinical routine laboratories. To support testing, quality controls and reference materials are needed. Those may be derived from residual patient samples, left over samples of external quality assurance schemes, plasmid DNA or DNA from cell lines. DNAs from cell lines are commutable and available in large amounts. METHODS: DNA from 38 cell lines were examined for suitability as controls in 11 SNP assays that are frequently used in a clinical routine laboratory: FV (1691G>A), FII (20210G>A), PAI-1 4G/5G polymorphism, MTHFR (677C>T, 1298A>C), HFE (H63D, S65C, C282Y), APOE (E2, E3, E4), LPH (-13910C>T), UGT1A1 (*28, *36, *37), TPMT (*2, *3A, *3B, *3C), VKORC1 (-1639G>A, 1173C>T), CYP2C9 (*2, *3, *5)...
May 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/29736094/influence-of-plasminogen-activator-inhibitor-1-gene-polymorphism-on-renal-scarring-after-first-febrile-urinary-tract-infection-in-infants
#9
Danka Pokrajac, Lejla Kapur-Pojskic, Sandra Vegar-Zubovic, Renata Milardovic
Background: The pathogenesis of renal scarring (RS) after first febrile urinary tract infection (UTI) in children is multifactorial. In addition to well-known risk factors, a role for genetic predisposition has been suggested. Aims: To determine whether deoxyribonucleic acid (DNA) polymorphisms at the plasminogen activator inhibitor -1 (PAI-1) gene were associated with evolution to RS following a febrile UTI in infants. Materials and Methods: Our research included 100 infants, 84 girls and 16 boys, ages up to 1 year with a first febrile UTI, increased inflammatory parameters and positive urine culture treated at the Pediatric Clinic II of the University Clinical Center Sarajevo (UCCS)...
April 2018: Medical Archives
https://www.readbyqxmd.com/read/29727174/exposure-to-vinyl-chloride-and-its-influence-on-western-diet-induced-cardiac-remodeling
#10
Yaqin Liang, Anna L Lang, Jian Zhang, Jing Chen, Kai Wang, Liya Chen, Juliane I Beier, Yan Qian, Lu Cai
Obesity, usually caused by high fat diets (HFD), is a major public health issue worldwide, causing obesity associated cardiomyopathy. Moreover, the environmental toxicant vinyl chloride (VC) can exacerbate HFD-induced fatty liver disease. However, whether VC serves to enhance obesity-associated cardiomyopathy remains unclear. This study aims to investigate the interaction of western diet (WD) containing relatively low fat (42%) with VC on cardiac remodeling and its underling mechanisms. Adult male C57BL/6J mice were exposed to WD coinhalation of low-dose VC (<1 ppm/d) for 12 weeks...
May 10, 2018: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/29724147/characteristics-of-fibrinolytic-disorders-in-acute-promyelocytic-leukemia
#11
Ping Wang, Yingmei Zhang, Huiyuan Yang, Wenyi Hou, Bo Jin, Jinxiao Hou, Haitao Li, Hongli Zhao, Jin Zhou
OBJECTIVES: Catastrophic hemorrhage remains the main cause of acute promyelocytic leukemia (APL) treatment failure. This study was aimed to study the pathogenesis of coagulopathy in patients with APL. METHODS: Multiple procoagulant and profibrinolytic parameters in plasma and peripheral leukocytes from 24 patients with newly diagnosed APL accompanied by coagulopathy before and after arsenic trioxide (ATO) treatment were evaluated. RESULTS: Prior to the treatment, the patients had elevated D-dimer and decreased fibrinogen levels...
May 4, 2018: Hematology (Amsterdam, Netherlands)
https://www.readbyqxmd.com/read/29717906/relationship-of-selected-adipokines-with-markers-of-vascular-damage-in-patients-with-type-2-diabetes
#12
Jaromíra Spurná, David Karásek, Veronika Kubíčková, Dominika Goldmannová, Ondřej Krystyník, Jan Schovánek, Josef Zadražil
BACKGROUND: In this study we compared levels of selected adipokines between patients with type 2 diabetes (T2D) and healthy individuals and we determined their relationship with early vascular damage markers. METHODS: Seventy-seven subjects: 56 patients with T2D (34 men and 22 women) and 21 healthy controls (8 men and 13 women) were examined in this cross-sectional study. Selected adipokines [adiponectin, adipocyte fatty acid-binding protein (A-FABP), fibroblast growth factor 21 (FGF-21), C1q/TNF-related protein 9 (CTRP-9), and allograft inflammatory factor-1 (AIF-1)] with possible cardiovascular impact were measured in all participants...
May 2, 2018: Metabolic Syndrome and related Disorders
https://www.readbyqxmd.com/read/29709004/mcu-knockdown-attenuates-high-glucose-induced-inflammation-through-regulating-mapks-nf-%C3%AE%C2%BAb-pathways-and-ros-production-in-hepg2-cells
#13
Ghodratollah Panahi, Parvin Pasalar, Mina Zare, Rosario Rizzuto, Reza Meshkani
Mitochondrial Ca2+ is a key regulator of organelle physiology and the excessive increase in mitochondrial calcium is associated with the oxidative stress. In the present study, we investigated the molecular mechanisms linking mitochondrial calcium to inflammatory and coagulative responses in hepatocytes exposed to high glucose (HG) (33mM glucose). Treatment of HepG2 cells with HG for 24 h induced insulin resistance, as demonstrated by an impairment of insulin-stimulated Akt phosphorylation. HepG2 treatment with HG led to an increase in mitochondrial Ca2+ uptake, while cytosolic calcium remained unchanged...
2018: PloS One
https://www.readbyqxmd.com/read/29708806/ashitaba-angelica-keiskei-exudate-prevents-increases-in-plasminogen-activator-inhibitor-1-induced-by-obesity-in-tsumura-suzuki-obese-diabetic-mice
#14
Mitsuhiro Ohta, Aya Fujinami, Katsutaka Oishi, Norihiro Kobayashi, Katsunori Ohnishi, Naoki Ohkura
Angelica keiskei koidzumi (ashitaba) is consumed as a traditional folk medicine and health food in Japan. Ashitaba extract contains abundant flavonoids containing chalcones. Plasminogen activator inhibitor-1 (PAI-1) is the primary physiological inhibitor of tissue plasminogen activator. Excessive amounts of PAI-1 in plasma disrupt the fibrinolytic balance and promote a prothrombotic state with which thrombosis and cardiovascular diseases are associated. In the present study, we investigated the effects of ashitaba yellow exudate (AE) on enhanced PAI-1 levels in Tsumura Suzuki obese diabetic (TSOD) mice...
April 30, 2018: Journal of Dietary Supplements
https://www.readbyqxmd.com/read/29703881/the-association-of-factor-v-g1961a-factor-v-leiden-prothrombin-g20210a-mthfr-c677t-and-pai-1-4g-5g-polymorphisms-with-recurrent-pregnancy-loss-in-bosnian-women
#15
Amela Jusić, Devleta Balić, Aldijana Avdić, Maja Pođanin, Adem Balić
Aim To investigate association of factor V Leiden, prothrombin G20210A, MTHFR C677T and PAI-1 4G/5G polymorphisms with recurrent pregnancy loss in Bosnian women. Methods A total of 60 women with two or more consecutive miscarriages before 20 weeks of gestation with the same partners and without history of known causes or recurrent pregnancy loss were included. A control group included 80 healthy women who had one or more successful pregnancies without history of any complication which could be associated with miscarriages...
August 1, 2018: Medicinski Glasnik
https://www.readbyqxmd.com/read/29702223/an-integrative-approach-to-investigate-the-association-among-high-sensitive-c-reactive-protein-body-fat-mass-distribution-and-other-cardiometabolic-risk-factors-in-young-healthy-women
#16
Bin Wu, Jingshan Huang, Lihua Zhang, Mohan Vamsi Kasukurthi, Fangwan Huang, Jiang Bian, Keisuke Fukuo, Tsutomu Kazumi
Prior research has indicated that as an important biomarker of chronic low-grade inflammation, high-sensitivity C-reactive protein (hs-CRP) can play important roles on the onset of metabolic syndrome and cardiovascular diseases (CVD). We conducted an integrative approach, which combines biological wet-lab experiments, statistical analysis, and semantics-oriented bioinformatics & computational analysis, to investigate the association among hs-CRP, body fat mass (FM) distribution, and other cardiometabolic risk factors in young healthy women...
April 24, 2018: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29700097/prostaglandin-e2-receptor-3-ep3-signaling-is-induced-in-placentas-with-unexplained-recurrent-pregnancy-losses-urpl
#17
Yao Ye, Aurelia Vattai, Nina Ditsch, Christina Kuhn, Martina Rahmeh, Sven Mahner, Myriam Ripphahn, Roland Immler, Markus Sperandio, Udo Jeschke, Viktoria von Schoenfeldt
Although an inflammatory microenvironment is required for successful implantation, an inflammatory overreaction is one of the causes of unexplained recurrent pregnancy losses (uRPL). Prostaglandin E2 (PGE2) plays a pivotal role in regulating immune balance during early pregnancy and it can stimulate inflammatory reactions via EP3. However, the role of PGE2 receptor signaling in the uRPL remains unknown. We aimed to investigate whether prostaglandin E2 receptor 3 (EP3) signaling is involved in the mechanism of uRPL...
April 26, 2018: Endocrine Connections
https://www.readbyqxmd.com/read/29693741/pai-1-caix-and-vegfa-expressions-as-prognosis-markers-in-oral-squamous-cell-carcinoma
#18
Gabriela Tonini Peterle, Lucas Lima Maia, Leonardo Oliveira Trivilin, Mayara Mota de Oliveira, Joaquim Gasparini Dos Santos, Suzanny Oliveira Mendes, Elaine Stur, Lidiane Pignaton Agostini, Lília Alves Rocha, Raquel Ajub Moysés, Patrícia Maluf Cury, Fábio Daumas Nunes, Iúri Drumond Louro, Marcelo Dos Santos, Adriana Madeira Álvares da Silva
BACKGROUND: In oral squamous cell carcinoma (OSCC), the HIF-1 complex promotes the expression of genes involved in specific mechanisms of cell survival under hypoxic conditions, such as plasminogen activator inhibitor-1 (PAI-1), carbonic anhydrase 9 (CAIX) and vascular endothelial growth factor A (VEGFA). The study aimed to investigate the presence and prognostic value of PAI-1, CAIX, and VEGFA in OSCC. MATERIALS AND METHODS: Immunohistochemistry was used to analyze the expressions of these proteins in 52 tumoral tissue samples of patients with OSCC, surgically treated and followed by a minimum of 24 months after surgery...
April 25, 2018: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/29693219/reduction-of-premature-aging-markers-after-gastric-bypass-surgery-in-morbidly-obese-patients
#19
P J Hohensinner, C Kaun, B Ebenbauer, M Hackl, S Demyanets, D Richter, M Prager, J Wojta, Gersina Rega-Kaun
BACKGROUND: Obesity is considered to be a major comorbidity. Obese patients suffer from an increased proinflammatory state associated with a premature aging phenotype including increased secretion of senescence-associated secretory proteins (SASP) and reduced telomere length. Micro-ribonucleic acids (miRNAs) are non-coding RNA molecules that could modify the post-transcriptional process. Several studies have reported associations between miRNAs and metabolic unhealthy conditions. AIM: To determine if bariatric surgery and the resulting weight loss could reverse the premature aging phenotype...
April 25, 2018: Obesity Surgery
https://www.readbyqxmd.com/read/29691454/heterologous-dna-prime-protein-boost-immunization-with-reca-and-flid-offers-cross-clade-protection-against-leptospiral-infection
#20
Veerapandian Raja, Sankaran Sobana, Charles Solomon Akino Mercy, Bianca Cotto, Durlav Prasad Bora, Kalimuthusamy Natarajaseenivasan
The emergence of >300 serovars of Leptospira confounded the use of generalized bacterin, the whole cell lysate, as vaccines to control leptospirosis. Because of substantial genetic and geographic heterogeneity among circulating serovars, one vaccine strain per serovar cannot be efficacious against all the serovars. We have performed heterologous DNA prime-protein boost vaccination challenge studies in hamsters using in vivo expressed, leptospiral recombinase A (RecA) and flagellar hook associated protein (FliD)...
April 24, 2018: Scientific Reports
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