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https://www.readbyqxmd.com/read/28811674/regenerative-medicine-and-cell-based-approaches-to-restore-pancreatic-function
#1
REVIEW
Cara Ellis, Adam Ramzy, Timothy J Kieffer
The pancreas is a complex organ with exocrine and endocrine components. Many pathologies impair exocrine function, including chronic pancreatitis, cystic fibrosis and pancreatic ductal adenocarcinoma. Conversely, when the endocrine pancreas fails to secrete sufficient insulin, patients develop diabetes mellitus. Pathology in either the endocrine or exocrine pancreas results in devastating economic and personal consequences. The current standard therapy for treating patients with type 1 diabetes mellitus is daily exogenous insulin injections, but cell sources of insulin provide superior glycaemic regulation and research is now focused on the goal of regenerating or replacing β cells...
August 16, 2017: Nature Reviews. Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/28811006/improved-fat-transplantation-survival-by-using-the-conditioned-medium-of-vascular-endothelial-growth-factor-transfected-human-adipose-derived-stem-cells
#2
Yang Zhang, Li-Ling Xiao, Jiang-Xuan Li, Hong-Wei Liu, Sheng-Hong Li, Yan-Yun Wu, Xuan Liao, Cong-Qiang Rao
Autologous fat transplantation has been applied widely in clinic. However, the low survival rate is still a problem to be solved. Studies shows that the human adipose-derived stem cells (ADSCs) transfected by vascular endothelial growth factor (VEGF) can improve the survival rate of autologous fat transplantation. Our study is to evaluate the effects of the conditioned medium of VEGF-transfected human adipose-derived stem cells (VEGF-ADSCs-CM) on fat transplantation. ADSCs were isolated and transfected with MOI = 40...
August 2017: Kaohsiung Journal of Medical Sciences
https://www.readbyqxmd.com/read/28810264/mesenchymal-stem-cells-promote-diabetic-corneal-epithelial-wound-healing-through-tsg-6-dependent-stem-cell-activation-and-macrophage-switch
#3
Guohu Di, Xianli Du, Xia Qi, Xiaowen Zhao, Haoyun Duan, Suxia Li, Lixin Xie, Qingjun Zhou
Purpose: To explore the role and mechanism of bone marrow-derived mesenchymal stem cells (BM-MSCs) in corneal epithelial wound healing in type 1 diabetic mice. Methods: Diabetic mice were treated with subconjunctival injections of BM-MSCs or recombinant tumor necrosis factor-α-stimulated gene/protein-6 (TSG-6). The corneal epithelial wound healing rate was examined by fluorescein staining. The mRNA and protein expression levels of TSG-6 were measured by quantitative RT-PCR and Western blot...
August 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28809024/chicken-induced-pluripotent-stem-cells-establishment-and-characterization
#4
Aurelie Fuet, Bertrand Pain
In mammals, the introduction of the OSKM (Oct4, Sox2, Klf4, and c-Myc) genes into somatic cells has allowed generating induced pluripotent stem (iPS) cells. So far, this process has been only clearly demonstrated in mammals. Here, using chicken as an avian model, we describe a set of protocols allowing the establishment, characterization, maintenance, differentiation, and injection of putative reprogrammed chicken Induced Pluripotent Stem (iPS) cells.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28809019/lentiviral-mediated-transgenesis-in-songbirds
#5
Wan-Chun Liu, Marian Hruska-Plochan, Atsushi Miyanohara
Transgenesis involves the insertion of an exogenous gene into an animal's genome, which allows the identification of the expressed phenotypes in brain function or behavior. Lentiviral-mediated transgenesis offers unique transduction potency making it possible to deliver and stably integrate transgenes into a wide variety of dividing and nondividing cells. The ability to establish long-term expression of such transgenes allows their use for transgenesis which is especially useful in organisms lacking quality pluripotent stem cell lines and which is otherwise difficult to produce via traditional pronuclear microinjection, such as songbirds...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28807497/characterisation-of-the-porcine-cytokines-which-activate-the-cd131%C3%AE-c-common-sub-unit-for-potential-immune-augmentation
#6
G Stephenson, K R Morris, T E O'Neil, M P Bruce, A D G Strom, A G D Bean
Early acting cytokines and growth factors such as those of the CD131 βc subunit, may offer an alternative method to the current use of antibiotics and chemicals such as anthelmintics in maintaining Porcine (Po) health. Thus far, the recombinant Po (rPo) Granulocyte-macrophage colony-stimulating factor (GM-CSF), rPo interleukin-3 (IL-3) and rPo interleukin-5 (IL-5) proteins have been identified and cloned and the biological activity of each cytokine has been confirmed in vitro, however, in vivo immune system regulation and hematopoietic stem cell (HSC) augmentation are regulated by numerous cytokines and cellular signals within the bone marrow (BM) niche...
August 11, 2017: Cytokine
https://www.readbyqxmd.com/read/28807034/exosomes-from-embryonic-mesenchymal-stem-cells-alleviate-osteoarthritis-through-balancing-synthesis-and-degradation-of-cartilage-extracellular-matrix
#7
Yafei Wang, Dongsheng Yu, Zhiming Liu, Fang Zhou, Jun Dai, Bingbing Wu, Jing Zhou, Boon Chin Heng, Xiao Hui Zou, Hongwei Ouyang, Hua Liu
BACKGROUND: Mesenchymal stem cell therapy for osteoarthritis (OA) has been widely investigated, but the mechanisms are still unclear. Exosomes that serve as carriers of genetic information have been implicated in many diseases and are known to participate in many physiological processes. Here, we investigate the therapeutic potential of exosomes from human embryonic stem cell-induced mesenchymal stem cells (ESC-MSCs) in alleviating osteoarthritis (OA). METHODS: Exosomes were harvested from conditioned culture media of ESC-MSCs by a sequential centrifugation process...
August 14, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28807014/epigenetic-reprogramming-converts-human-wharton-s-jelly-mesenchymal-stem-cells-into-functional-cardiomyocytes-by-differential-regulation-of-wnt-mediators
#8
G Bhuvanalakshmi, Frank Arfuso, Alan Prem Kumar, Arun Dharmarajan, Sudha Warrier
BACKGROUND: Lineage commitment of mesenchymal stem cells (MSCs) to cardiac differentiation is controlled by transcription factors that are regulated by epigenetic events, mainly histone deacetylation and promoter DNA methylation. Here, we studied the differentiation of human Wharton's jelly MSCs (WJMSCs) into the cardiomyocyte lineage via epigenetic manipulations. METHODS: We introduced these changes using inhibitors of DNA methyl transferase and histone deacetylase, DC301, DC302, and DC303, in various combinations...
August 14, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28807003/overexpression-of-mir-21-in-stem-cells-improves-ovarian-structure-and-function-in-rats-with-chemotherapy-induced-ovarian-damage-by-targeting-pdcd4-and-pten-to-inhibit-granulosa-cell-apoptosis
#9
Xiafei Fu, Yuanli He, Xuefeng Wang, Dongxian Peng, Xiaoying Chen, Xinran Li, Qing Wang
BACKGROUND: Chemotherapy-induced premature ovarian failure (POF) is a severe complication affecting tumor patients at a childbearing age. Mesenchymal stem cells (MSCs) can partially restore the ovarian structure and function damaged by chemotherapy. miR-21 is a microRNA that can regulate cell apoptosis. This study discusses the repair effect and mechanism of MSCs overexpressing miR-21 on chemotherapy-induced POF. METHODS: Rat MSCs and granulosa cells (GCs) were isolated in vitro...
August 14, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28805297/mesenchymal-stem-cells-deliver-exogenous-mir-21-via-exosomes-to-inhibit-nucleus-pulposus-cell-apoptosis-and-reduce-intervertebral-disc-degeneration
#10
Xiaofei Cheng, Guoying Zhang, Liang Zhang, Ying Hu, Kai Zhang, Xiaojiang Sun, Changqing Zhao, Hua Li, Yan Michael Li, Jie Zhao
Although mesenchymal stem cells (MSCs) transplantation into the IVD (intervertebral disc) may be beneficial in inhibiting apoptosis of nucleus pulposus cells (NPCs) and alleviating IVD degeneration, the underlying mechanism of this therapeutic process has not been fully explained. The purpose of this study was to explore the protective effect of MSC-derived exosomes (MSC-exosomes) on NPC apoptosis and IVD degeneration and investigate the regulatory effect of miRNAs in MSC-exosomes and associated mechanisms for NPC apoptosis...
August 14, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28805262/mesenchymal-stem-cells-attenuate-liver-fibrosis-by-suppressing-th17-cells
#11
Neda Milosavljevic, Marina Gazdic, Bojana Simovic Markovic, Aleksandar Arsenijevic, Jasmin Nurkovic, Zana Dolicanin, Nemanja Jovicic, Ilija Jeftic, Valentin Djonov, Nebojsa Arsenijevic, Miodrag L Lukic, Vladislav Volarevic
This study investigates molecular and cellular mechanisms involved in mesenchymal stem cell (MSCs)-mediated modulation of IL-17 signaling during liver fibrosis. Mice received CCl4 (1μL/g intraperitoneally) twice/week for 1 month. MSCs (1x10(6) ), or MSC-conditioned medium (MSC-CM) were intravenously injected 24h after CCl4 and on every 7(th) day. Liver fibrosis was determined by macroscopic examination, histological analysis, Sirius red staining and RT-PCR. Serum levels of cytokines, IDO and kynurenine were determined by ELISA...
August 14, 2017: Transplant International: Official Journal of the European Society for Organ Transplantation
https://www.readbyqxmd.com/read/28804608/remyelination-improvement-after-neurotrophic-factors-secreting-cells-transplantation-in-rat-spinal-cord-injury
#12
Shahnaz Razavi, Nazem Ghasemi, Mohammad Mardani, Hossein Salehi
OBJECTIVES: Neurotrophic factors secreting cells (NTS-SCs) may be a superior cell source for cell-based therapy in neurodegenerative diseases. NTS-SCs are able to secrete some neurotrophic Such as nerve growth factor and glia-derived neurotrophic factor. Our primary aim was to assess transplantation of neurotrophic factor secreting cells derived from human adipose-derived stem cells (hADSCs) into the damaged spinal cord rats and determine the potential of these cells in remyelination...
April 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28804549/treatment-effect-of-low-intensity-pulsed-ultrasound-on-leukopenia-induced-by-cyclophosphamide-in-rabbits
#13
Baoru Liu, Yueping Luo, Dong Luo, Weichen Zhou, Yu Zhang, Ruixin He, Junshu Li, Yong Wang, Yan Wang, Wenzhi Chen
OBJECTIVE: This study aims to examine the effects of low intensity pulsed ultrasound (LIPUS) on leukopenia induced by cyclophosphamide in a rabbit model. METHODS: The leukopenia model in New Zealand rabbit was established by injecting cyclophosphamide into the ear vein. Forty leukopenia model rabbits were randomly allocated to control group (n = 20) and LIPUS group (n = 20). LIPUS group underwent 20 minutes of daily ultrasound treatment at femoral metaphysis for 7 days while control group received sham treatment...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28803917/sca1-cells-from-the-heart-possess-a-molecular-circadian-clock-and-display-circadian-oscillations-in-cellular-functions
#14
Bastiaan C Du Pré, Evelyne J Demkes, Dries A M Feyen, Pieterjan Dierickx, Sandra Crnko, Bart J M Kok, Joost P G Sluijter, Pieter A Doevendans, Marc A Vos, Toon A B Van Veen, Linda W Van Laake
Stem cell antigen 1-positive (SCA1(+)) cells (SPCs) have been investigated in cell-based cardiac repair and pharmacological research, although improved cardiac function after injection has been variable and the mode of action remains unclear. Circadian (24-hr) rhythms are biorhythms regulated by molecular clocks that play an important role in (patho)physiology. Here, we describe (1) the presence of a molecular circadian clock in SPCs and (2) circadian rhythmicity in SPC function. We isolated SPCs from human fetal heart and found that these cells possess a molecular clock based on typical oscillations in core clock components BMAL1 and CRY1...
August 8, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28803602/short-and-long-term-therapeutic-efficacies-of-intravenous-transplantation-of-bone-marrow-stem-cells-on-cardiac-function-in-rats-with-acute-myocardial-infarction-a-meta-analysis-of-randomized-controlled-trials
#15
Can Jiang, Dong Zheng, Yun-Lu Feng, Jun Guo, Hai-Rui Li, Ai-Dong Zhang
Objective To investigate the short- and long-term therapeutic efficacies of intravenous trans- plantation of bone marrow stem cells (MSCs) in rats with experimental myocardial infarction by meta- analysis. Methods Randomized controlled trials were systematically searched from PubMed, Science Citation Index (SCI), Chinese journal full-text database (CJFD) up to December 2014. While the experimental groups (MSCs groups) were injected MSCs intravenously, the control groups were injected Delubecco's minimum essential medium (DMEM) or phosphate buffered saline (PBS)...
September 20, 2016: Chinese Medical Sciences Journal, Chung-kuo i Hsüeh K'o Hsüeh Tsa Chih
https://www.readbyqxmd.com/read/28801914/blockade-of-transforming-growth-factor-%C3%AE-signaling-enhances-oncolytic-herpes-simplex-virus-efficacy-in-patient-derived-recurrent-glioblastoma-models
#16
Shinichi Esaki, Fares Nigim, Esther Moon, Samantha Luk, Juri Kiyokawa, William Curry, Daniel P Cahill, Andrew S Chi, A John Iafrate, Robert L Martuza, Samuel D Rabkin, Hiroaki Wakimoto
Despite the current standard of multimodal management, glioblastoma (GBM) inevitably recurs and effective therapy is not available for recurrent disease. A subset of tumor cells with stem-like properties, termed GBM stem-like cells (GSCs), are considered to play a role in tumor relapse. Although oncolytic herpes simplex virus (oHSV) is a promising therapeutic for GBM, its efficacy against recurrent GBM is incompletely characterized. Transforming growth factor beta (TGF-β) plays vital roles in maintaining GSC stemness and GBM pathogenesis...
August 12, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28800128/adult-cardiac-stem-cells-are-multipotent-and-robustly-myogenic-c-kit-expression-is-necessary-but-not-sufficient-for-their-identification
#17
Carla Vicinanza, Iolanda Aquila, Mariangela Scalise, Francesca Cristiano, Fabiola Marino, Eleonora Cianflone, Teresa Mancuso, Pina Marotta, Walter Sacco, Fiona C Lewis, Liam Couch, Victoria Shone, Giulia Gritti, Annalaura Torella, Andrew J Smith, Cesare Mn Terracciano, Domenico Britti, Pierangelo Veltri, Ciro Indolfi, Bernardo Nadal-Ginard, Georgina M Ellison-Hughes, Daniele Torella
Multipotent adult resident cardiac stem cells (CSCs) were first identified by the expression of c-kit, the stem cell factor receptor. However, in the adult myocardium c-kit alone cannot distinguish CSCs from other c-kit-expressing (c-kit(pos)) cells. The adult heart indeed contains a heterogeneous mixture of c-kit(pos) cells, mainly composed of mast and endothelial/progenitor cells. This heterogeneity of cardiac c-kit(pos) cells has generated confusion and controversy about the existence and role of CSCs in the adult heart...
August 11, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28798777/mesenchymal-stem-cells-promote-metastasis-of-lung-cancer-cells-by-downregulating-systemic-antitumor-immune-response
#18
Marina Gazdic, Bojana Simovic Markovic, Nemanja Jovicic, Maja Misirkic-Marjanovic, Valentin Djonov, Vladimir Jakovljevic, Nebojsa Arsenijevic, Miodrag L Lukic, Vladislav Volarevic
Since majority of systemically administered mesenchymal stem cells (MSCs) become entrapped within the lungs, we used metastatic model of lung cancer, induced by intravenous injection of Lewis lung cancer 1 (LLC1) cells, to investigate the molecular mechanisms involved in MSC-mediated modulation of metastasis. MSCs significantly augmented lung cancer metastasis, attenuate concentrations of proinflammatory cytokines (TNF-α, IL-17), and increase levels of immunosuppressive IL-10, nitric oxide, and kynurenine in sera of LLC1-treated mice...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28798327/assessing-cell-nanoparticle-interactions-by-high-content-imaging-of-biocompatible-iron-oxide-nanoparticles-as-potential-contrast-agents-for-magnetic-resonance-imaging
#19
Roxanne Hachani, Martin A Birchall, Mark W Lowdell, Georgios Kasparis, Le D Tung, Bella B Manshian, Stefaan J Soenen, Willy Gsell, Uwe Himmelreich, Codi A Gharagouzloo, Srinivas Sridhar, Nguyen T K Thanh
Stem cell tracking in cellular therapy and regenerative medicine is an urgent need, superparamagnetic iron oxide nanoparticles (IONPs) could be used as contrast agents in magnetic resonance imaging (MRI) that allows visualization of the implanted cells ensuring they reach the desired sites in vivo. Herein, we report the study of the interaction of 3,4-dihydroxyhydrocinnamic acid (DHCA) functionalized IONPs that have desirable properties for T2 - weighted MRI, with bone marrow-derived primary human mesenchymal stem cells (hMSCs)...
August 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28795668/-immune-intervention-of-human-umbilical-cord-mesenchymal-stem-cells-on-sepsis-rats
#20
Hewei Zhang, Xiaoxu Cui, Tao Fang, Qiang Fu
OBJECTIVE: To investigate the effect of human umbilical cord mesenchymal stem cells (UC-MSCs) on immune cells and inflammatory factors in septic rats. METHODS: 184 male Sprague-Dawley (SD) rats were divided into normal control group (n = 8), sham operation group (n = 48), sepsis model group (n = 64), and UC-MSCs treatment group (n = 64). An animal model of sepsis was produced by cecal ligation and puncture (CLP). In the UC-MSCs treatment group 1 mL UC-MSCs (2×10(6)/mL) were injected intraperitoneally at 1 hour after the model establishment; the sham operation group and the sepsis model group were given the same amount of saline...
August 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
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