keyword
MENU ▼
Read by QxMD icon Read
search

Chromatography

keyword
https://www.readbyqxmd.com/read/29471542/specialized-pro-resolving-lipid-mediators-regulate-ozone-induced-pulmonary-and-systemic-inflammation
#1
Brita Kilburg-Basnyat, Sky W Reece, Miranda J Crouch, Bin Luo, Andria D Boone, Michael Yaeger, Myles Hodge, Christine Psaltis, Johanna L Hannan, Jonathan Manke, Michael L Armstrong, Nichole Reisdorph, Robert M Tighe, Saame Raza Shaikh, Kymberly M Gowdy
Exposure to ozone (O3) induces lung injury, pulmonary inflammation, and alters lipid metabolism. During tissue inflammation, specialized pro-resolving lipid mediators (SPMs) facilitate the resolution of inflammation. SPMs regulate the pulmonary immune response during infection and allergic asthma; however, the role of SPMs in O3-induced pulmonary injury and inflammation is unknown. We hypothesized that O3 exposure induces pulmonary inflammation by reducing SPMs. To evaluate this, male C57Bl/6J mice were exposed to filtered air (FA) or 1 ppm O3 for 3h and necropsied 24h after exposure...
February 19, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29471466/changes-in-metabolites-present-in-lung-lining-fluid-following-exposure-of-humans-to-ozone
#2
WanYun Cheng, Kelly E Duncan, Andrew J Ghio, Cavin Ward-Caviness, Edward D Karoly, David Diaz-Sanchez, Rory B Conolly, Robert B Devlin
Controlled human exposure to the oxidant air pollutant ozone causes decrements in lung function and increased inflammation as evidenced by neutrophil influx into the lung and increased levels of proinflammatory cytokines in the airways. Here we describe a targeted metabolomics evaluation of human bronchioalveolar lavage fluid (BALF) following controlled in vivo exposure to ozone to gain greater insight into its pulmonary effects. In a two-arm cross-over study, each healthy adult human volunteer was randomly exposed to filtered air (FA) and to 0...
February 19, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29471241/influence-of-chelation-on-the-fenton-based-electrochemical-degradation-of-herbicide-tebuthiuron
#3
Fábio Gozzi, Ignasi Sirés, Silvio César de Oliveira, Amílcar Machulek, Enric Brillas
This study describes the performance of electro-Fenton (EF) and photoelectro-Fenton (PEF) processes to degrade the herbicide tebuthiuron (TBH) in 0.050 M Na2 SO4 at pH = 3.0. Experiments were performed in an undivided cell equipped with a boron-doped diamond (BDD) or Pt anode and an air-diffusion cathode that produces H2 O2 . Physisorbed hydroxyl radicals (M(OH)) generated from water oxidation at the anode and/or free OH formed from Fenton's reaction acted as main oxidants. All processes became much more effective using a BDD anode because of the higher oxidation power of BDD(OH)...
February 12, 2018: Chemosphere
https://www.readbyqxmd.com/read/29471230/variations-of-floc-morphology-and-extracellular-organic-matters-eom-in-relation-to-floc-filterability-under-algae-flocculation-harvesting-using-polymeric-titanium-coagulants-ptcs
#4
Weijun Zhang, Rongna Song, Bingdi Cao, Xiaofang Yang, Dongsheng Wang, Xingmin Fu, Yao Song
The work evaluated the algae cells removal efficiency using titanium salt coagulants with different degree of polymerization (PTCs), and the algae cells aggregates and extracellular organic matter (EOM) under chemical flocculation were investigated. The results indicated that PTCs performed well in algae cells flocculation and separation. The main mechanism using PTCs of low alkalisation degree for algae flocculation was associated with charge neutralization, while adsorption bridging and sweep flocculation was mainly responsible for algae removal by PTCs of high alkalisation degree treatment...
February 10, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29471165/emission-characteristics-of-pcdd-fs-in-stack-gas-from-municipal-solid-waste-incineration-plants-in-northern-china
#5
Feng Zhu, Xiaofei Li, Jia-Wei Lu, Jing Hai, Jieru Zhang, Bing Xie, Chengyang Hong
Emission characteristics including congener's profile, gas emissions and toxic equivalent concentration (TEQ) indicators of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in 57 stack gas samples from 6 municipal solid waste incinerators (MSWIs) in Northern China were investigated by gas chromatography-high resolution mass spectrometry (HRGC-HRMS). Additionally, PCDD/Fs formation mechanisms from the MSWIs were briefly discussed. Results revealed that the concentrations and equivalent concentrations of PCDD/Fs emissions in stack gas from 6 MSWIs were in the range of 0...
February 16, 2018: Chemosphere
https://www.readbyqxmd.com/read/29471092/purification-and-functional-characterization-of-recombinant-balsamin-a-ribosome-inactivating-protein-from-momordica-balsamina
#6
Parminder K Ajji, Shailendra P Sonkar, Ken Walder, Munish Puri
Balsamin, a type I ribosome-inactivating protein (RIP), has been shown to inhibit HIV-1 replication at the translation step. Our recent studies have shown that balsamin also possess anti-tumor, antibacterial and DNase-like activity, however, the amount of natural balsamin in Momordica balsamina seeds is limited and preclinical studies require large quantities of pure, bioactive balsamin. Therefore, in this study, we cloned the balsamin gene, expressed it in E. coli BL21(DE3) strain and purified it by nickel affinity chromatography...
February 19, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29471088/characterization-of-highly-branched-dextran-produced-by-leuconostoc-citreum-b-2-from-pineapple-fermented-product
#7
Fang Feng, Qingqing Zhou, Yanfang Yang, Fangkun Zhao, Renpeng Du, Ye Han, Huazhi Xiao, Zhijiang Zhou
A strain Leuconostoc citreum B-2 was isolated from homemade fermentation product of pineapple and its polysaccharide yield was 28.3 g/L after cultivating the strain in Man-Rogosa-Sharpe (MRS) medium with 75 g/L sucrose. The exopolysaccharide (EPS) was characterized by gas chromatography (GC), Fourier-transform infrared (FT-IR) spectra, high-performance size-exclusion chromatography (HPSEC), nuclear magnetic resonance (NMR) spectroscopy and scanning electron microscope (SEM) analysis in present study. The monosaccharide composition of EPS was glucose and molecular weight was 3...
February 19, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29471066/a-pharmacokinetic-study-on-lapatinib-in-type-2-diabetic-rats
#8
Agnieszka Karbownik, Edyta Szałek, Katarzyna Sobańska, Agnieszka Klupczynska, Szymon Plewa, Tomasz Grabowski, Anna Wolc, Marta Moch, Zenon J Kokot, Edmund Grześkowiak
BACKGROUND: Diabetes mellitus (DM) is a complex metabolic disorder which affects the function of numerous tissues and alters the pharmacokinetic parameters of many drugs. As many oncological patients are diabetics, it is important to determine the influence of this chronic disease on the pharmacokinetics (PK) of anticancer drugs. Lapatinib is a tyrosine kinase inhibitor (TKI), approved for the treatment of human epidermal growth factor receptor 2 (HER2)-positive metastatic breast cancer...
September 18, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/29471007/risk-assessment-of-benzene-in-food-samples-of-iran-s-market
#9
Ali Heshmati, Sabah Ghadimi, Amin Mousavi Khaneghah, Francisco J Barba, Jose M Lorenzo, Fatemeh Nazemi, Yadolah Fakhri
The concentration of benzene in ninety-eight collected food and drink samples (carbonated beverage, fruit juice, pickle, lime juice, mayonnaise and salad dressing, 16 samples from each) from Iran local markets were investigated using gas chromatography equipped with flame ionization detector (GC-FID). Moreover, the correlation of benzene concentration with sodium benzoate and ascorbic acid concentrations was assessed. Benzene concentration in carbonated beverages, fruit juices, pickle, lime juices, mayonnaise and salad dressing were 3...
February 19, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29470948/direct-analysis-of-pi-3-4-5-p-3-using-liquid-chromatography-electrospray-ionization-tandem-mass-spectrometry
#10
Hai H Bui, Phillip E Sanders, Diane Bodenmiller, Ming Shang Kuo, Gregory P Donoho, Anthony S Fischl
Phosphatidylinositol (3,4,5) trisphosphate (PIP3 ) is a biologically active membrane phospholipid that is essential for the growth and survival of all eukaryotic cells. We describe a new method that directly measures PIP3 and describe the HPLC separation and measurement of the positional isomers of phosphatidylinositol bisphosphate, PI(3,5)P2 , PI(3,4)P2 and PI(4,5)P2 . Mass spectrometric analyses were performed online using ultra-high performance liquid chromatography (UHPLC)-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) in the negative multiple-reaction monitoring (MRM) modes...
February 19, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29470860/investigation-of-the-in-vivo-metabolism-of-harpagoside-and-distribution-of-its-metabolites-in-rats-by-hplc-it-tof-ms-n
#11
Jing-Zhe Wang, Yi-Fan Zhang, Feng Xu, Ming-Ying Shang, Guang-Xue Liu, Shao-Qing Cai
Harpagoside, an iridoid glycoside, is the major bioactive constituent of the traditional Chinese medicine Scrophulariae Radix. High-performance liquid chromatography with a diode array detector combined with electrospray ionization ion trap time-of-flight multistage mass spectrometry (HPLC-ESI-IT-TOF-MSn ) was used to profile and identify the metabolites of harpagoside in rats in vivo and to study the distribution of these metabolites in rats for the first time. A total of 45 metabolites were identified, 37 of which were postulated to be new compounds...
February 22, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29470848/simultaneous-determination-of-evodiamine-and-its-four-metabolites-in-rat-plasma-by-lc-ms-ms-and-its-application-to-a-pharmacokinetic-study
#12
Cuiyun Wang, Fang Yue, Guangfeng Ai, Jie Yang
A simple and sensitive liquid chromatography tandem mass spectrometry method was validated for simultaneous quantification of evodiamine and its metabolites 10-hydroxyevodiamine (M1), 18-hydroxyevodiamine (M2), 10-hydroxyevodiamine-glucuronide (M3) and 18-hydroxy- evodiamine-glucuronide (M4) in rat plasma for the first time. The analytes were extracted with acetonitrile and separated on a C18 column within 3 min. The detection was achieved in positive selected reaction monitoring mode with precursor-to-product transitions at m/z 304...
February 22, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29470816/hepatic-subcellular-distribution-of-squalene-changes-according-to-the-experimental-setting
#13
Roberto Martínez-Beamonte, Olga Alda, Teresa Sanclemente, María J Felices, Sara Escusol, Carmen Arnal, Luis V Herrera-Marcos, Sonia Gascón, Joaquín C Surra, Jesús Osada, Mª Jesús Rodríguez-Yoldi
Squalene is the main unsaponifiable component of virgin olive oil, the main source of dietary fat in Mediterranean diet, traditionally associated with a less frequency of cardiovascular diseases. In this study, two experimental approaches were used. In the first, New Zealand rabbits fed for 4 weeks with a chow diet enriched in 1% sunflower oil for the control group, and in 1% of sunflower oil and 0.5% squalene for the squalene group. In the second, APOE KO mice received either Western diet or Western diet enriched in 0...
February 22, 2018: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/29470751/method-development-for-determination-of-migrated-phthalate-acid-esters-from-polyethylene-terephthalate-pet-packaging-into-traditional-iranian-drinking-beverage-doogh-samples-a-novel-approach-of-mspe-gc-ms-technique
#14
Amin Kiani, Mahsa Ahmadloo, Nabi Shariatifar, Mojtaba Moazzen, Abbas Norouzian Baghani, GholamReza Jahed Khaniki, Ali Taghinezhad, Amin Kouhpayeh, Amin Mousavi Khaneghah, Peyman Ghajarbeygi
In the current study, a novel magnetic solid phase extraction (MSPE) technique combined with a gas chromatography/mass spectroscopy (GC/MS) was developed to determine the phthalate ester content of bottled Doogh samples. Doogh is a yogurt-based drinking beverage, which is frequently consumed in Middle East and Balkans. It is produced by stirring yogurt in Chern separation machine and consists of substances such as water, yogurt, and salt in addition to aqueous extracts of native herbs. The magnetic multi-walled carbon nanotubes (MWCNT-Fe3 O4 ) were used as adsorbents of phthalate acid esters (PAEs) due to a superior adsorption capability of hydrophobic compounds...
February 22, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29470664/toward-miniaturized-analysis-of-chemical-identity-and-purity-of-radiopharmaceuticals-via-microchip-electrophoresis
#15
Jimmy Ly, Noel S Ha, Shilin Cheung, R Michael van Dam
Miniaturized synthesis of positron emission tomography (PET) tracers is poised to offer numerous advantages including reduced tracer production costs and increased availability of diverse tracers. While many steps of the tracer production process have been miniaturized, there has been relatively little development of microscale systems for the quality control (QC) testing process that is required by regulatory agencies to ensure purity, identity, and biological safety of the radiotracer before use in human subjects...
February 22, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29470511/preliminary-analysis-of-proteome-alterations-in-non-aneurysmal-internal-mammary-artery-tissue-from-patients-with-abdominal-aortic-aneurysms
#16
Christina Lund Kidholm, Hans Christian Beck, Julie Bukh Madsen, Nikolai Bjødstrup Palstrøm, Jes Sanddal Lindholt, Lars Melholt Rasmussen
OBJECTIVE: The pathogenesis of abdominal aortic aneurysms (AAA) involves a disturbed balance of breakdown and buildup of arterial proteins. We envision that individuals with AAA carry generalized arterial protein alterations either because of effects of genetically or environmental AAA risk factors or because of compensatory changes due to signaling molecules released from the affected aneurysmal tissue. APPROACH: Protein extraction and quantitative proteome analysis by LC-MS/MS (liquid chromatography-mass spectrometry) was done on individual samples from the internal mammary artery from 11 individuals with AAA and 33 sex- and age-matched controls without AAA...
2018: PloS One
https://www.readbyqxmd.com/read/29470453/uhplc-uv-method-for-simultaneous-determination-of-perindopril-arginine-and-indapamide-hemihydrate-in-combined-dosage-form-a-stability-indicating-assay-method
#17
Naser F Al-Tannak
Perindopril arginine and Indapamide hemihydrate in combination were proven to have a synergistic antihypertensive impact when compared with the use of each component alone. Therefore, a new Ultra-High Performance Liquid Chromatography coupled with Ultraviolet detector (UHPLC-UV) method has been developed and subsequently validated for simultaneous determination of the anti-hypertensive combination of Perindopril arginine and Indapamide hemihydrate. The separation of Perindopril arginine and Indapamide hemihydrate was achieved using a BEH C18 (1...
February 22, 2018: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/29470443/structure-antiplatelet-activity-relationships-of-novel-ruthenium-ii-complexes-investigation-of-its-molecular-targets
#18
Chih-Hsuan Hsia, Thanasekaran Jayakumar, Joen-Rong Sheu, Shin-Yi Tsao, Marappan Velusamy, Chih-Wei Hsia, Duen-Suey Chou, Chao-Chien Chang, Chi-Li Chung, Themmila Khamrang, Kao-Chang Lin
The regulation of platelet function by pharmacological agents that modulate platelet signaling has proven to be a positive approach to the prevention of thrombosis. Ruthenium complexes are fascinating for the development of new drugs, as they possess numerous chemical and biological properties. The present study aims to evaluate the structure-activity relationship (SAR) of newly synthesized ruthenium (II) complexes, TQ-1, TQ-2 and TQ-3 in agonists-induced washed human platelets. Silica gel column chromatography, aggregometry, immunoblotting, NMR, and X-ray analyses were performed in this study...
February 22, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29470438/altered-expression-of-ganglioside-metabolizing-enzymes-results-in-gm3-ganglioside-accumulation-in-cerebellar-cells-of-a-mouse-model-of-juvenile-neuronal-ceroid-lipofuscinosis
#19
Aleksandra Somogyi, Anton Petcherski, Benedikt Beckert, Mylene Huebecker, David A Priestman, Antje Banning, Susan L Cotman, Frances M Platt, Mika O Ruonala, Ritva Tikkanen
Juvenile neuronal ceroid lipofuscinosis (JNCL) is caused by mutations in the CLN3 gene. Most JNCL patients exhibit a 1.02 kb genomic deletion removing exons 7 and 8 of this gene, which results in a truncated CLN3 protein carrying an aberrant C-terminus. A genetically accurate mouse model ( Cln3 Δex7/8 mice) for this deletion has been generated. Using cerebellar precursor cell lines generated from wildtype and Cln3 Δex7/8 mice, we have here analyzed the consequences of the CLN3 deletion on levels of cellular gangliosides, particularly GM3, GM2, GM1a and GD1a...
February 22, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29469990/differential-metabolic-pathway-analysis-of-the-proteomes-of-leishmania-donovani-and-leptomonas-seymouri
#20
Neeloo Singh, Renu Goel, Ekta Jain
PURPOSE: Although in trypanosomatids, monoxeny (Leptomonas) is ancestral to dixeny (Leishmania), however clinical cases of visceral leishmanisis with Leptomonas co-infection are increasingly being reported from India. Using a proteogenomic approach, we carried out a detailed proteome analysis of these two kinetoplastid parasites viz. Leishmania and its sister Leptomonas, to catalog the key proteins associated with and therefore possibly responsible for phenotype changes in Leptomonas evolution and domestication as co-infection with Leishmania...
February 22, 2018: Proteomics. Clinical Applications
keyword
keyword
29345
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"