keyword
MENU ▼
Read by QxMD icon Read
search

Chromatography

keyword
https://www.readbyqxmd.com/read/28550805/enantioselective-accumulation-metabolism-and-phytoremediation-of-lactofen-by-aquatic-macrophyte-lemna-minor
#1
Fang Wang, Xiaotong Yi, Han Qu, Li Chen, Donghui Liu, Peng Wang, Zhiqiang Zhou
Pesticides are frequently detected in water bodies due to the agricultural application, which may pose impacts on aquatic organisms. The enantioselective bioaccumulation and metabolism of the herbicide lactofen in aquatic floating macrophyte Lemna minor (L. minor) were studied and the potential L. minor phytoremediation was investigated. Ultra-high performance liquid chromatography - tandem mass spectrometry (UHPLC-MS-MS) analysis for lactofen and its two known metabolites in L. minor was performed. The initial concentrations of racemic lactofen, R-lactofen and S-lactofen were all 30μgL(-1) in the growth solution...
May 24, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28550781/on-the-behavior-of-reduced-graphene-oxide-based-electrodes-coated-with-dispersed-platinum-by-alternate-current-methods-in-the-electrochemical-degradation-of-reactive-dyes
#2
A I Del Río, C García, J Molina, J Fernández, J Bonastre, F Cases
The electrochemical behavior of different carbon-based electrodes with and without nanoparticles of platinum electrochemically dispersed on their surface has been studied. Among others, reduced graphene oxide based electrodes was used to determine the best conditions for the decolorization/degradation of the reactive dye C.I. Reactive Orange 4 in sulfuric medium. Firstly, the electrochemical behavior was evaluated by cyclic voltammetry. Secondly, different electrolyses were performed using two cell configurations: cell with anodic and cathodic compartments separated (divided configuration) and without any separation (undivided configuration)...
May 22, 2017: Chemosphere
https://www.readbyqxmd.com/read/28550728/quantification-of-nitrous-oxide-in-wastewater-based-on-salt-induced-stripping
#3
Pascal Kosse, Manfred Lübken, Torsten C Schmidt, Marc Wichern
Monitoring nitrous oxide (N2O) emissions from wastewater treatment plants has attracted much attention in recent years demanding accurate and rapid quantification methods. In the present study a salt-assisted methodology is proposed by which N2O is chemically stripped out from wastewater and quantified by gas chromatography (GC-TCD) subsequently. Eight different inorganic salts have been evaluated for this purpose, likewise the application of ultrasound. By addition of sodium bromide (NaBr) the best recovery rate of about 98% (=1...
May 24, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28550716/ex-vivo-measurement-of-scalp-follicular-infundibulum-delivery-of-zinc-pyrithione-and-climbazole-from-an-anti-dandruff-shampoo
#4
Guoqiang Chen, Chengdong Ji, Miao Miao, Kang Yang, Yajun Luo, Michael Hoptroff, Luisa Z Collins, Hans-Gerd Janssen
Efficient delivery of anti-dandruff (AD) actives into the scalp follicular infundibulum as well as onto the scalp surface is critical for the efficacy of AD shampoos. A method involving scalp cyanoacrylate (CA) biopsy sampling, a tailor made cutting device, ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis, scanning electron microscopy (SEM) measurement and Raman imaging has been developed for the measurement of delivery of zinc pyrithione (ZPT) and climbazole (CBZ) from an AD shampoo into the scalp follicular infundibulum...
May 17, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28550651/a-new-biphenyl-ether-derivative-produced-by-indonesian-ascidian-derived-penicillium-albobiverticillium
#5
Deiske A Sumilat, Hiroyuki Yamazaki, Kotaro Endo, Henki Rotinsulu, Defny S Wewengkang, Kazuyo Ukai, Michio Namikoshi
A new biphenyl ether derivative, 2-hydroxy-6-(2'-hydroxy-3'-hydroxymethyl-5-methylphenoxy)-benzoic acid (1), was isolated together with the known benzophenone derivative, monodictyphenone (2), from a culture broth of Indonesian ascidian-derived Penicillium albobiverticillium TPU1432 by solvent extraction, ODS column chromatography, and preparative HPLC (ODS). The structure of 1 was elucidated based on NMR experiments. Compound 2 exhibited moderate inhibitory activities against protein tyrosine phosphatase (PTP) 1B, T cell PTP (TCPTP), and CD45 tyrosine phosphatase (CD45), whereas compound 1 modestly inhibited CD45 activity...
May 26, 2017: Journal of Natural Medicines
https://www.readbyqxmd.com/read/28550642/post-heroin-dose-tissue-distribution-of-6-monoacetylmorphine-6-mam-with-maldi-imaging
#6
Belin G Teklezgi, Annapurna Pamreddy, Sooraj Baijnath, Nirmala D Gopal, Tricia Naicker, Hendrik G Kruger, Thavendran Govender
Heroin is an illicit opioid drug which is commonly abused and leads to dependence and addiction. Heroin is considered a pro-drug and is rapidly converted to its major active metabolite 6-monoacetylmorphine (6-MAM) which mediates euphoria and reward through the stimulation of opioid receptors in the brain. The aim of this study was to investigate the distribution and localization of 6-MAM in the healthy Sprague Dawley rat brain following intraperitoneal (i.p) administration of heroin (10 mg/kg), using matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI), in combination with quantification via liquid chromatography mass spectrometry (LC-MS/MS)...
May 26, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28550633/the-efficiency-of-the-multi-walled-carbon-nanotubes-used-for-antibiotics-removal-from-wastewaters-generated-by-animal-farms
#7
Maria-Loredana Soran, Ocsana Opriş, Ildikó Lung, Irina Kacso, Alin S Porav, Manuela Stan
In the recent years, residual antibiotics are considered to be emerging environmental pollutants due to their continuous input and persistence into the aquatic ecosystem even at low concentrations. Therefore, these are necessary to develop efficient methods for the wastewater treatment. The present paper describes the efficiency of several types of multi-walled carbon nanotubes (MWCNTs) for the retention of the selected antibiotics (ampicillin, ceftazidime, cefepime, imipenem, piperacillin, tazobactam, tetracycline, erythromycin, ciprofloxacin, norfloxacin, vancomycin, gentamicin, sulfamethoxazole, and thrimetoprim) from aqueous (synthetic) solutions and wastewater samples...
May 27, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28550445/%C3%AE-carotene-at-physiologically-attainable-concentration-induces-apoptosis-and-down-regulates-cell-survival-and-antioxidant-markers-in-human-breast-cancer-mcf-7-cells
#8
G Sowmya Shree, K Yogendra Prasad, H S Arpitha, U R Deepika, K Nawneet Kumar, Priya Mondal, P Ganesan
Although β-carotene is known for its anti-carcinogenic and antioxidant properties, a few recent epidemiological and experimental evidence show that at higher concentration it acts as pro-oxidant and induces cancer. Since the global burden of breast cancer exceeds all other types of cancer, and its incidence rates is also in increasing trend, the present study attempted to evaluate the anti-cancer molecular mechanism of β-carotene (at 1 µM concentration) isolated from Spinacia oleracea in human breast cancer (MCF-7) cells...
May 26, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28550302/antigenicity-of-bovine-pericardium-determined-by-a-novel-immunoproteomic-approach
#9
Katherine V Gates, Ailsa J Dalgliesh, Leigh G Griffiths
Despite bovine pericardium (BP) being the primary biomaterial used in heart valve bioprostheses, recipient graft-specific immune responses remain a significant cause of graft failure. Consequently, tissue antigenicity remains the principal barrier for expanding use of such biomaterials in clinical practice. We hypothesize that our understanding of BP antigenicity can be improved by application of a combined affinity chromatography shotgun immunoproteomic approach to identify antigens that have previously been overlooked...
May 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28550293/stochastic-sensing-of-angiotensin-ii-with-lysenin-channels
#10
Nisha Shrestha, Sheenah L Bryant, Christopher Thomas, Devon Richtsmeier, Xinzhu Pu, Juliette Tinker, Daniel Fologea
The ability of pore-forming proteins to interact with various analytes has found vast applicability in single molecule sensing and characterization. In spite of their abundance in organisms from all kingdoms of life, only a few pore-forming proteins have been successfully reconstituted in artificial membrane systems for sensing purposes. Lysenin, a pore-forming toxin extracted from the earthworm E. fetida, inserts large conductance nanopores in lipid membranes containing sphingomyelin. Here we show that single lysenin channels may function as stochastic nanosensors by allowing the short cationic peptide angiotensin II to be electrophoretically driven through the conducting pathway...
May 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28550166/lysine-acetylome-analysis-reveals-photosystem-ii-manganese-stabilizing-protein-acetylation-is-involved-in-negative-regulation-of-oxygen-evolution-in-model-cyanobacterium-synechococcus-sp-pcc-7002
#11
Zhuo Chen, Guiying Zhang, Mingkun Yang, Tao Li, Feng Ge, Jindong Zhao
Nϵ-Acetylation of lysine residues represents a frequently occurring post-translational modification widespread in bacteria that plays vital roles in regulating bacterial physiology and metabolism. However, the role of lysine acetylation in cyanobacteria remains unclear, presenting a hurdle to in-depth functional study of this post-translational modification. Here, we report the lysine acetylome of Synechococcus sp. PCC 7002 (hereafter Synechococcus) using peptide prefractionation, immunoaffinity enrichment, and coupling with high-precision liquid chromatography-tandem mass spectrometry analysis...
May 26, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28550076/high-throughput-analysis-of-sulfatides-in-cerebrospinal-spinal-fluid-using-automated-extraction-and-uplc-ms-ms
#12
Mara Blomqvist, Jan Bor Eacuten, Henrik Zetterberg, Kaj Blennow, Jan-Erik M Aringnsson, Marcus St Aringhlman
Sulfatides are a group of glycosphingolipids that are highly expressed in brain. Due to their importance for normal brain function and their potential involvement in neurological diseases, development of accurate and sensitive methods for their determination are needed. Here we describe a high-throughput oriented and quantitative method for the determination of sulfatides in cerebrospinal fluid. The sulfatides are extracted using a fully automated liquid/liquid extraction method and quantified using ultra-performance liquid chromatography coupled to tandem mass spectrometry...
May 26, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28549974/evaluation-of-tacrolimus-sorption-to-pvc-and-non-pvc-based-tubes-in-administration-sets-pump-method-vs-drip-method
#13
Su-Eon Jin, Seungho Jeon, Hyo-Jin Byon, Sung-Joo Hwang
Tacrolimus sorption to tubes was evaluated using pump and drip methods For tubes, polyvinylchloride (PVC)- and non-PVC-based (polyurethane [PU] and polyolefin [PO]) tubes were used. First, inner surface properties of tubes were analyzed using field emission scanning electron microscopy and X-ray photoelectron spectroscopy. Tacrolimus was quantitatively analyzed using high-performance liquid chromatography with UV detection. For kinetic sorption analysis, diluted tacrolimus to 10μg/mL was passed through 1-m-long tubes at 10mL/h...
May 23, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28549946/potential-metabolomic-biomarkers-for-reliable-diagnosis-of-behcet-s-disease-using-gas-chromatography-with-time-of-flight-mass-spectrometry
#14
Joong Kyong Ahn, Jungyeon Kim, Jiwon Hwang, Juhwan Song, Kyoung Heon Kim, Hoon-Suk Cha
OBJECTIVES: Although many diagnostic criteria of Behcet's disease (BD) have been developed and revised by experts, diagnosing BD is still complicated and challenging. No metabolomic studies on serum have been attempted to improve the diagnosis and to identify potential biomarkers of BD. The purposes of this study were to investigate distinctive metabolic changes in serum samples of BD patients and to identify metabolic candidate biomarkers for reliable diagnosis of BD using the metabolomics platform...
May 23, 2017: Joint, Bone, Spine: Revue du Rhumatisme
https://www.readbyqxmd.com/read/28549866/venom-of-the-peruvian-snake-bothriopsis-oligolepis-detection-of-antibacterial-activity-and-involvement-of-proteolytic-enzymes-and-c-type-lectins-in-growth-inhibition-of-staphylococcus-aureus
#15
M A Sulca, C Remuzgo, J Cárdenas, S Kiyota, E Cheng, M P Bemquerer, M T Machini
There is a rising interest in snake venoms proteins (SVPs) because these macromolecules are related to pharmacological properties that manifest themselves during poisoning and can lead to secondary microbial infections. Interestingly, researchers have somehow neglected the antimicrobial activity of SVPs. The aims of this study were: (i) to verify whether the venom of the Peruvian snake Bothriopsis oligolepis displays such activity; (ii) to isolate and identify some its antimicrobial constituents. Liquid growth inhibition assays revealed that the crude venom inhibited the growth of Gram-positive and Gram-negative bacteria, but not of Candida species...
May 23, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28549859/ethnopharmacological-investigations-of-the-cardio-renal-properties-of-a-native-species-from-the-region-of-pantanal-state-of-mato-grosso-do-sul-brazil
#16
Cleide Adriane Signor Tirloni, Francislaine Aparecida Dos Reis Lívero, Rhanany Alan Calloi Palozi, Renan César Alves Silveira, Paulo César de Paula Vasconcelos, Roosevelt Isaias Carvalho Souza, Ariany Carvalho Dos Santos, Valter Paes de Almeida, Jane Manfron Budel, Lauro Mera de Souza, Arquimedes Gasparotto Junior
ETHNOPHARMACOLOGICAL RELEVANCE: Acanthospermum hispidum DC. is an important medicinal herb that belongs to family Asteraceae, popularly used as a diuretic and hypotensive in the region of Pantanal, state of Mato Grosso do Sul, Brazil. Despite the relevance of this species throughout the country, there are no detailed studies about its possible ethnobotanical indication. AIM: To carry out a detailed ethnopharmacological investigation of the cardio-renal properties of A...
May 23, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28549717/separation-of-alk-en-ylresorcinols-from-rye-bran-with-saturated-monoenoic-dienoic-trienoic-and-hydroxylated-monoenoic-side-chains-using-an-octyl-phase-in-ultra-high-performance-liquid-chromatography-and-their-differentiation-by-tandem-mass-spectrometrie
#17
Benno F Zimmermann, Hannes Patzke, Andreas Schieber
Alkylresorcinols (ARs) occur in bran of cereals and in fruits from the Anacardiaceae family. Their separation by liquid chromatography is challenging, especially in rye (Secale cereale L.) that has a complex AR composition. An octyl phase (C8) with 1.8μm particles was used for the analysis of an acetone extract of rye bran. The ARs were detected by UV at 205 and 275nm and by MS applying selected ion monitoring (SIM) of known and hypothetical m/z values in positive and negative mode. The compounds found were subjected to product ion scans in a triple quadrupole mass spectrometer...
May 15, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28549716/automated-programmable-pressurized-carbonic-acid-eluent-ion-exclusion-chromatography-of-organic-acids
#18
C Phillip Shelor, Purnendu K Dasgupta
Conductometric ion exclusion chromatography of weak acids does not enjoy the same degree of success as its counterpart in suppressed conductometric anion exchange chromatography. Compared to the latter, eluent preparation is not automated. Commonly, a strong acid is used as the eluent leading to a relatively high-detector background and poorer limits of detection. In addition, generally applicable gradient elution has not been possible. We introduce the automated preparation of carbonic acid eluents at concentrations exceeding 0...
May 16, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28549715/direct-residue-analysis-of-systemic-insecticides-and-some-of-their-relevant-metabolites-in-wines-by-liquid-chromatography-mass-spectrometry
#19
J D Berset, S Mermer, A E Robel, V M Walton, M L Chien, J A Field
A direct large volume injection (DI-LVI) high performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the quantitative determination of 16 systemic insecticides and their main plant metabolites. The assays were conducted on commercial red and white wines made from grapes grown in major wine-producing regions nationally and internationally. Using a 1:20 dilution and an injection volume of 800μL, a limit of quantitation (LOQ) of 1μgL(-1) for all analytes was achieved...
May 13, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28549656/glycyrrhizin-and-glycyrrhetinic-acid-inhibits-alpha-naphthyl-isothiocyanate-induced-liver-injury-and-bile-acid-cycle-disruption
#20
Haina Wang, Zhong-Ze Fang, Ran Meng, Yun-Feng Cao, Naoki Tanaka, Kristopher W Krausz, Frank J Gonzalez
Alpha-naphthyl isothiocyanate (ANIT) is a common hepatotoxicant experimentally used to reproduce the pathologies of drug-induced liver injury in humans, but the mechanism of its toxicity remains unclear. To determine the metabolic alterations following ANIT exposure, metabolomic analyses was performed by use of liquid chromatography-mass spectrometry. Partial least squares discriminant analysis (PLS-DA) of liver, serum, bile, ileum, and cecum of vehicle- and ANIT-treated mice revealed significant alterations of individual bile acids, including increased tauroursodeoxycholic acid, taurohydrodeoxycholic acid, taurochenodeoxycholic acid, and taurodeoxycholic acid, and decreased ω-, β- and tauro-α/β- murideoxycholic acid, cholic acid, and taurocholic acid in the ANIT-treated groups...
May 23, 2017: Toxicology
keyword
keyword
29345
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"