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https://www.readbyqxmd.com/read/28110389/a-novel-lectin-from-artocarpus-lingnanensis-induces-proliferation-and-th1-th2-cytokine-secretion-through-cd45-signaling-pathway-in-human-t-lymphocytes
#1
Bo Cui, Lu Li, Qiyan Zeng, Faquan Lin, Lijun Yin, Liejun Liao, Min Huang, Jingping Wang
Lectins are carbohydrate-binding proteins and have been used for purification and characterization of glycoproteins. In this study, a novel 58.9-kDa tetrameric lectin from Artocarpus lingnanensis seeds was purified, characterized, and its mitogenic potential was evaluated. The hemagglutination inhibition assay indicated that Artocarpus lingnanensis lectin (ALL) showed specificity toward galactose. ALL was effectively purified in a single-step using affinity chromatography on a galactose-Sepharose column. ALL showed pH optima between 5...
January 21, 2017: Journal of Natural Medicines
https://www.readbyqxmd.com/read/28110246/fe-fe2o3-promoted-electrochemical-mineralization-of-atrazine-via-a-triazinon-ring-opening-mechanism
#2
Xing Ding, Shengyao Wang, Wanqiu Shen, Yi Mu, Li Wang, Hao Chen, Lizhi Zhang
In this study, an electrochemical/electro-Fenton oxidation (EC/EF) system was designed to degrade atrazine, by utilizing boron-doped diamond (BDD) and Fe@Fe2O3 core-shell nanowires loaded active carbon fiber (Fe@Fe2O3/ACF) as the anode and the cathode, respectively. This EC/EF system exhibited much higher degradation rate, decholorination and mineralization efficiency of atrazine than the electrochemical (EC) and electrochemical/traditional electro-Fenton (EC/TEF) oxidation counterpart systems without Fe@Fe2O3 core-shell nanowires...
January 16, 2017: Water Research
https://www.readbyqxmd.com/read/28110168/ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry-uhplc-ms-ms-for-determination-of-ghb-precursors-and-metabolites-in-different-specimens-application-to-clinical-and-forensic-cases
#3
Francesco Paolo Busardò, Chrystalla Kyriakou, Emilia Marchei, Roberta Pacifici, Daniel Sejer Pedersen, Simona Pichini
Gamma-hydroxybutyric acid (GHB) acts as a precursor and metabolite of the inhibitory central nervous system (CNS) neurotransmitter gamma-aminobutyric acid (GABA). Sodium salt of GHB has been used as a medication for narcolepsy and alcohol withdrawal. Moreover, GHB and its precursor gamma-butyrolactone (GBL), are illegal recreational drugs of abuse. A procedure based on ultra-high-performance liquid chromatography tandem mass spectrometry has been developed and validated in plasma, urine, cerebrospinal fluid and hair for acute and chronic exposure to GHB and in seized preparations coming from black market...
January 9, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28110167/chiral-separation-of-new-sulfonamide-derivatives-and-evaluation-of-their-enantioselective-affinity-for-human-carbonic-anhydrase-ii-by-microscale-thermophoresis-and-surface-plasmon-resonance
#4
Tiphaine Rogez-Florent, Catherine Foulon, Anne-Sophie Drucbert, Nadège Schifano, Perrine Six, Stéphanie Devassine, Patrick Depreux, Pierre-Marie Danzé, Laurence Goossens, Cécile Danel, Jean-François Goossens
The aim of this study was to develop a method combining chiral separation and biophysical techniques to evaluate the enantioselective affinity of original sulfonamide derivatives towards their therapeutic target, the human carbonic anhydrase II (hACII). The first step consisted in the preparation of the enantiomers by chromatographic separation. The performances of HPLC and Supercritical Fluid Chromatography (SFC) were studied at the analytical scale by optimization of various experimental conditions using adsorbed polysaccharide chiral stationary phases (amylose AD-H and cellulose OD-H)...
January 9, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28110081/isolation-and-partial-characterization-of-an-immunogenic-antigen-of-giardia-lamblia
#5
Jael Quintero, Alejandra Valdez, Brenda Samaniego, Gloria Lopez-Romero, Humberto Astiazaran-Garcia, Lucila Rascon, Linda Breci, Adriana Garibay-Escobar, Ramón Robles-Zepeda, Carlos Velazquez
Humoral and cellular immune responses play an important role during Giardia lamblia infection. Several Giardia proteins have been identified as immunogenic antigens based on their elicited humoral immune response. Poorly is known about Giardia antigens that stimulate a cellular immune response. The main purpose of this study was to isolate and partial characterize an immunogenic antigen (5G8) of G. lamblia. The 5G8 protein was isolated from G. lamblia trophozoite lysates by affinity chromatography using moAb 5G8-coupled CNBr-Sepharose...
January 18, 2017: Parasitology International
https://www.readbyqxmd.com/read/28110033/purification-characterization-and-functional-analysis-of-the-immune-molecule-lectin-from-the-haemolymph-of-blue-swimmer-crab-portunus-pelagicus-and-their-antibiofilm-properties
#6
Sangily Jayanthi, Ramachandran Ishwarya, Mahalingam Anjugam, Arokiadhas Iswarya, Sivashanmugam Karthikeyan, Baskaralingam Vaseeharan
The present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab Portunus pelagicus (Pp-Lec). The Pp-Lec was purified by affinity chromatography with mannose coupled sepharose CL-4B column and it exhibits single band with a molecular weight of 155 kDa in SDS-PAGE. The surface morphology of purified Pp-Lec displays the homogeneous nature of protein. A distinct peak with a retention time of 3.3 min was appeared in high performance liquid chromatography (HPLC) and X-ray diffraction (XRD) analysis expresses a single peak at 31...
January 18, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28110016/spatial-characteristics-of-urinary-btex-concentrations-in-the-general-population
#7
Xanthi Tsangari, Xanthi D Andrianou, Agapios Agapiou, Pawel Mochalski, Konstantinos C Makris
Benzene, toluene, ethylbenzene, o-, m-, and p-xylenes (BTEX) are ubiquitous outdoor and indoor air pollutants associated with both environmental and health effects. The objective of this exploratory study was to determine the magnitude and variability of urinary BTEX levels among residents of two areas located in the same city (Nicosia, Cyprus). The two areas differed with respect to their proximity to an industrial cluster and an intercity-highway. First morning urine voids were collected during a random campaign from selected households in the two urban areas (n = 48)...
January 8, 2017: Chemosphere
https://www.readbyqxmd.com/read/28109982/altered-glutamate-metabolism-contributes-to-antiepileptogenic-effects-in-the-progression-from-focal-seizure-to-generalized-seizure-by-low-frequency-stimulation-in-the-ventral-hippocampus
#8
Hong-Liu Sun, Wei Zhu, Yu-Rong Zhang, Xiao-Hong Pan, Jun-Ru Zhang, Xiang-Ming Chen, Yu-Xia Liu, Shu-Cui Li, Qiao-Yun Wang, Da-Ping Deng
As a promising method for treating intractable epilepsy, the inhibitory effect of low-frequency stimulation (LFS) is well known, although its mechanisms remain unclear. Excessive levels of cerebral glutamate are considered a crucial factor for epilepsy. Therefore, we designed experiments to investigate the crucial parts of the glutamate cycle. We evaluated glutamine synthetase (GS, metabolizes glutamate), glutaminase (synthesizes glutamate), and glutamic acid decarboxylase (GAD, a γ-aminobutyric acid [GABA] synthetase) in different regions of the brain, including the dentate gyrus (DG), CA3, and CA1 subregions of the hippocampus, and the cortex, using western blots, immunohistochemistry, and enzyme activity assays...
January 18, 2017: Epilepsy & Behavior: E&B
https://www.readbyqxmd.com/read/28109917/assessment-of-collagen-quality-associated-with-non-enzymatic-cross-links-in-human-bone-using-fourier-transform-infrared-imaging
#9
F N Schmidt, E A Zimmermann, G M Campbell, G E Sroga, K Püschel, M Amling, S Y Tang, D Vashishth, B Busse
Aging and many disease conditions, most notably diabetes, are associated with the accumulation of non-enzymatic cross-links in the bone matrix. The non-enzymatic cross-links, also known as advanced glycation end products (AGEs), occur at the collagen tissue level, where they are associated with reduced plasticity and increased fracture risk. In this study, Fourier-transform infrared (FTIR) imaging was used to detect spectroscopic changes associated with the formation of non-enzymatic cross-links in human bone collagen...
January 18, 2017: Bone
https://www.readbyqxmd.com/read/28109808/selective-adsorption-of-pb-ii-ions-by-amylopectin-g-poly-acrylamide-co-acrylic-acid-a-bio-degradable-graft-copolymer
#10
Dinabandhu Sasmal, Jayanta Maity, Haradhan Kolya, Tridib Tripathy
Amylopectin-g-poly (acrylamide-co-acrylic acid) [AP-g-poly (AM-co-AA)] was synthesised in water medium by using potassium perdisulphate as an initiator. The graft copolymer was characterized by molecular weight determination by size exclusion chromatography (SEC), fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) spectroscopy, scanning electron microscope (SEM) studies, thermal analysis, measurement of neutralisation equivalent and biodegradation studies. The graft copolymer was used for Pb (II) ion removal from aqueous solution...
January 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28109528/specific-enrichment-of-a-targeted-nitrotyrosine-containing-peptide-from-complex-matrices-and-relative-quantification-for-liquid-chromatography-mass-spectrometry-analysis
#11
Yun Yang
Protein tyrosine nitration is considered an important non-enzymatic post-translational modification. In the tyrosine nitration process, 3-nitrotyrosine is formed and recognized as a biomarker of nitrosative/nitrative stress implicated in inflammatory responses and age-related disorders. In view of the complexity of biological samples and the ultra-low abundance of protein-incorporated nitrotyrosine, selective enrichment of nitrotyrosine-containing peptides prior to chromatographic separation is crucial. Herein, I report a simple yet highly specific and efficient enrichment method for nitrotyrosine-containing peptides...
January 15, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28109489/impact-of-environmental-volatile-organic-compounds-on-otitis-media-in-children-correlation-between-exposure-and-urinary-metabolites
#12
So Young Kim, Bu-Soon Son, Hee-Jin Park, Seung Ha Oh, Jun Ho Lee, Myung-Hwan Suh, Moo Kyun Park
INTRODUCTION: Volatile organic compounds (VOCs) induce inflammatory responses. Tobacco smoke contains numerous VOCs and is a risk factor for otitis media effusion (OME); however, no previous studies have investigated the association between VOCs and OME. OBJECTIVES: We used urinary metabolites and exposure to environmental risk factors to investigate the association between VOC and polycyclic aromatic hydrocarbon exposure and recurrent OME in children. METHODS: Children with recurrent OME who visited the Otorhinolaryngology Department of Seoul National University Hospital between November 2014 and June 2015 were prospectively enrolled in the study...
February 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28109443/separation-of-two-distinct-o-glycoforms-of-human-iga1-by-serial-lectin-chromatography-followed-by-mass-spectrometry-o-glycan-analysis
#13
S Lehoux, T Ju
Human immunoglobulin A1 (IgA1), which carries four to six mucin-type O-glycans (O-glycans) on its hinge region (HR), is the most abundant O-glycoprotein in plasma or serum. While normal O-glycans from hematopoietic-originated cells are core 1-based complex structures, many reports showed that the IgA1 from patients with IgA nephropathy (IgAN) carries undergalactosylated or truncated O-glycans such as the Tn antigen and its sialylated version the SialylTn (STn) antigen on the HR. Yet, there is still a debate whether Tn/STn on the HR of IgA1 is specific to the IgA1 from patients with IgAN since these antigens have also been seen in serum IgA1 of healthy individuals...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28109439/deep-profiling-of-proteome-and-phosphoproteome-by-isobaric-labeling-extensive-liquid-chromatography-and-mass-spectrometry
#14
B Bai, H Tan, V R Pagala, A A High, V P Ichhaporia, L Hendershot, J Peng
Mass spectrometry-based proteomics has experienced an unprecedented advance in comprehensive analysis of proteins and posttranslational modifications, with particular technical progress in liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and isobaric labeling multiplexing capacity. Here, we introduce a deep proteomics profiling protocol that combines 10-plex tandem mass tag (TMT) labeling with an optimized LC-MS/MS platform to quantitate whole proteome and phosphoproteome. The major steps include protein extraction and digestion, TMT labeling, two-dimensional liquid chromatography, TiO2-mediated phosphopeptide enrichment, high-resolution mass spectrometry, and computational data processing...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28109432/targeted-in-depth-quantification-of-signaling-using-label-free-mass-spectrometry
#15
P R Cutillas
Protein phosphorylation encodes information on the activity of kinase-driven signaling pathways that regulate cell biology. This chapter discusses an approach, named TIQUAS (targeted in-depth quantification of signaling), to quantify cell signaling comprehensively and without bias. The workflow-based on mass spectrometry (MS) and computational science-consists of targeting the analysis of phosphopeptides previously identified by shotgun liquid chromatography tandem MS (LC-MS/MS) across the samples that are being compared...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28109274/the-role-of-fatty-acids-on-icsi-outcomes-a-prospective-cohort-study
#16
Parvaneh Mirabi, Mohammad Javad Chaichi, Sedighe Esmaeilzadeh, Seyed Gholam Ali Jorsaraei, Ali Bijani, Mahjoobeh Ehsani, Seyedeh Fezzeh Hashemi Karooee
BACKGROUND: Our objective was to determine the effect of fatty acids (FAs) in serum and follicular fluid (FF) on fertilization and intracytoplasmic sperm injection (ICSI) outcomes. METHODS: One hundred five women aged 18-38 years undergoing ICSI were recruited in this prospective cohort study. oocyte and emberyo quality was morphologically assessed. FAs in serum and FF were analyzed, using gas chromatography-mass spectrometry (GC-MS). RESULTS: The mean number of mature oocytes was associated with serum levels of oleic acid (r = 0...
January 21, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28109126/a-step-wise-approach-to-define-binding-mechanisms-of-surrogate-viral-particles-to-multi-modal-anion-exchange-resin-in-a-single-solute-system
#17
Matthew Brown, Sarah Johnson, Kurt Brorson, Scott Lute, David Roush
Multi-modal anion exchange resins combine properties of both anion exchange and hydrophobic interaction chromatography for commercial protein polishing and may provide some viral clearance as well. From a regulatory viral clearance claim standpoint, it is unclear if multi-modal resins are truly orthogonal to either single-mode anion exchange or hydrophobic interaction columns. To answer this, a strategy of solute surface assays and High Throughput Screening of resin in concert with a scale-down model of large scale chromatography purification was employed to determine the predominant binding mechanisms of a panel of bacteriophage (i...
January 21, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28109117/lipid-profiling-of-parkin-mutant-human-skin-fibroblasts
#18
Simona Lobasso, Paola Tanzarella, Daniele Vergara, Michele Maffia, Tiziana Cocco, Angela Corcelli
Parkin mutations are a major cause of early-onset Parkinson's disease (PD). The impairment of protein quality control system together with defects in mitochondria and autophagy process are consequences of the lack of parkin, which leads to neurodegeneration. Little is known about the role of lipids in these alterations of cell functions. In the present study parkin-mutant human skin primary fibroblasts have been considered as cellular model of PD to investigate on possible lipid alterations associated with the lack of parkin protein...
January 21, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28109074/determination-of-four-nadolol-stereoisomers-in-capsules-by-high-performance-liquid-chromatography-and-circular-dichroism
#19
Grazielle Prado Alexandre, Márcio Nardelli Wandermuren, Adriano Peron, Vanessa Franco Tavares, Denis Alexandre Ramalho Padeiro, Kelly Alessandra Sakiara, María Segunda Aurora-Prado, Anil Kumar Singh, Erika Rosa Maria Kedor-Hackmann, Maria Inês Rocha Miritello Santoro
Nadolol is a blocking agent with activity in β-adrenergic receptors. The objective of this research was to develop andvalidate an HPLC and circular dichroism method for the quantification of four nadolol stereoisomers in capsules. The HPLC method was validated using a Chiralcel OD column (250 × 4,6 mm, 10 μm). The mobile phase consisted of hexane-ethanol-diethylamine-acetic acid(86 + 14 + 0.3 + 0.3, v/v/v/v), with a flow rate of 0.7 mL/min and temperature of 25 ± 1°C and UV detection carried out at 220 nm...
January 21, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28109042/expression-and-characterization-of-a-codon-optimized-blood-coagulation-factor-viii
#20
S A Shestopal, J-J Hao, E Karnaukhova, Y Liang, M V Ovanesov, M Lin, J H Kurasawa, T K Lee, J H McVey, A G Sarafanov
BACKGROUND: Production of recombinant factor VIII (FVIII) is challenging due to its low expression. It was previously shown that codon-optimization of a B domain-deleted FVIII (BDD-FVIII) cDNA resulted in increased protein expression. However, it is well-recognized that synonymous mutations may affect the protein structure and function. OBJECTIVES: To compare biochemical properties of a BDD-FVIII expressed from codon-optimized (CO) and the wild-type (WT) cDNAs. METHODS: Each variant of the BDD-FVIII was expressed in several independent CHO cell lines, generated using a lentiviral platform...
January 21, 2017: Journal of Thrombosis and Haemostasis: JTH
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