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https://www.readbyqxmd.com/read/29793133/an-ultrasensitive-and-selective-electrochemical-aptasensor-based-on-rgo-mwcnts-chitosan-carbon-quantum-dot-for-the-detection-of-lysozyme
#1
Behzad Rezaei, Hamid Reza Jamei, Ali Asghar Ensafi
An aptamer-based method is described for the electrochemical determination of lysozyme. A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS. The composition of the nanocomposite (rGO-MWCNT/CS/CQD) warrants a high surface-to-volume ratio, high conductivity, high stability, and great electrocatalytic activity. This nanocomposite provides a suitable site for better immobilization of aptamers due to the existence of many amino and carboxyl functional groups, and remaining oxygen-related defects properties in rGO...
May 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29793047/transcript-profiling-of-salt-tolerant-tobacco-mutants-generated-via-mutation-breeding
#2
Özge Çelik, Aybüke Ekşioğlu, Enes Yağız Akdaş
The main aim of the study is to identify the genes differentially, predominantly or specifically expressed in salt tolerant tobacco mutants, improved from Akhisar 97 and İzmir Özbaş varieties via mutation breeding, with respect to unstressed control plants. Seven tobacco mutants which have different salt tolerance capacities were evaluated by Gene Fishing analysis. Under stress conditions differentially expressed 100 reproducible bands were identified (74 of up-regulated and 20 of down-regulated while 6 were unknown)...
May 21, 2018: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29792967/validated-reverse-phase-hplc-diode-array-method-for-the-quantification-of-intact-bevacizumab-infliximab-and-trastuzumab-for-long-term-stability-study
#3
Antonio Martínez-Ortega, Agustín Herrera, Antonio Salmerón-García, José Cabeza, Luis Cuadros-Rodríguez, Natalia Navas
The aim of the present study was to develop suitable and reliable method for quantification three of the most worldwide used therapeutic monoclonal antibodies (mAbs) -bevazizumab (BVZ), infliximab (INF) and trastuzumab (TTZ)- to be used in long-term stability studies. Reverse phase (RP) was selected by its greater sensibility and reproducibility comparing with other chromatographic modes. Then a high performance liquid chromatography with diode array detection (RP)HPLC/DAD method was checked. Since the three mAbs represent the active ingredient in the medicines in which they are formulated, the selected method was validated for each one in accordance with the International Conference on Harmonization (ICH) guidelines for pharmaceuticals for human use...
May 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29792897/mathematical-modeling-and-numerical-simulation-of-the-mitotic-spindle-orientation-system
#4
Bashar Ibrahim
The mitotic spindle orientation and position is crucial for the fidelity of chromosome segregation during asymmetric cell division to generate daughter cells with different sizes or fates. This mechanism is best understood in the budding yeast Saccharomyces cerevisiae, named the spindle position checkpoint (SPOC). The SPOC inhibits cells from exiting mitosis until the mitotic spindle is properly oriented along the mother-daughter polarity axis. Despite many experimental studies, the mechanisms underlying SPOC regulation remains elusive and unexplored theoretically...
May 21, 2018: Mathematical Biosciences
https://www.readbyqxmd.com/read/29792800/effects-of-enhanced-flexibility-and-pore-size-distribution-on-adsorption-induced-deformation-of-mesoporous-materials
#5
Andrei Kolesnikov, Nikolaj Georgi, Yury A Budkov, Jens Möllmer, Jorg Hofmann, Juergen Adolphs, Roger Gläser
We present a new model of adsorption-induced deformation of mesoporous solids. The model is based on a simplified version of Local Density Functional Theory in the framework of solvation free energy. Instead of density, which is treated as constant here, we used the film thickness and pore radius as order parameters. This allows us to obtain a self-consistent system of equations describing simultaneously the processes of gas adsorption and adsorbent deformation, as well as conditions for capillary condensation and evaporation...
May 24, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29792796/nano-lc-nsi-ms-refines-lipidomics-by-enhancing-lipid-coverage-measurement-sensitivity-and-linear-dynamic-range
#6
Niklas Danne-Rasche, Cristina Coman, Robert Ahrends
Nano-liquid chromatography (nLC) - nano-electrospray (NSI) is one of the cornerstones of mass spectrometry based bioanalytics. Nevertheless, the application of nLC is not prevailing lipid analyses so far. In this study, we established a reproducible nLC separation for global lipidomics and describe the merits of using such a miniaturized system for lipid analyses. In order to enable comprehensive lipid analyses that is not restricted to specific lipid classes, we particularly optimized sample preparation conditions and reversed phase separation parameters...
May 24, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29792545/an-update-on-quality-control-for-the-pfa-100-pfa-200
#7
Emmanuel J Favaloro, Roslyn Bonar
Testing of platelet function comprises a crucial element of hemostasis assessment, particularly for investigations into bleeding and/or bruising. The Platelet Function Analyzer (PFA)-100 is the most utilized primary hemostasis-screening test system available, as recently remodeled/upgraded to the PFA-200. Internal quality control (IQC) and external quality assessment (EQA) (including proficiency testing) represent critical elements of ensuring test practice quality. Although true for all tests, IQC and EQA are logistically challenging for platelet function testing, inclusive of the PFA-100/200...
May 24, 2018: Platelets
https://www.readbyqxmd.com/read/29792503/revisiting-carroll-s-survey-of-factor-analytic-studies-implications-for-the-clinical-assessment-of-intelligence
#8
Nicholas F Benson, A Alexander Beaujean, Ryan J McGill, Stefan C Dombrowski
John Carroll's three-stratum theory (and the decades of research behind its development) is foundational to the contemporary practice of intellectual assessment. The present study addresses some limitations of Carroll's work: specification, reproducibility with more modern methods, and interpretive relevance. We reanalyzed select data sets from Carroll's survey of factor analytic studies using confirmatory factor analysis as well as modern indices of interpretive relevance. For the majority of data sets, we found that Carroll likely extracted too many factors representing Stratum II abilities...
May 24, 2018: Psychological Assessment
https://www.readbyqxmd.com/read/29792435/measuring-drug-induced-changes-in-metabolite-populations-of-live-bacteria-real-time-analysis-by-raman-spectroscopy
#9
Paul R Carey, Grant R Whitmer, Michael J Yoon, Michael N Lombardo, Marianne Pusztai-Carey, Hossein Heidari-Torkabadi, Tao Che
Raman difference spectroscopy is shown to provide a wealth of molecular detail on changes within bacterial cells caused by infusion of antibiotics or hydrogen peroxide. E. coli strains paired with chloramphenicol, DHFR propargyl-based inhibitors, meropenem, or hydrogen peroxide provide details of the depletion of protein and nucleic acid populations in real time. Additionally, other reproducible Raman features appear and are attributed to changes in cell metabolite populations. An initial candidate for one of the metabolites involves population increases of citrate, an intermediate within the TCA cycle...
May 24, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29792323/cellular-beacons-mediated-counting-for-ultrasensitive-detection-of-ebola-virus-on-an-integrated-micromagnetic-platform
#10
Shao-Li Hong, Ya-Nan Zhang, Ya-Hua Liu, Man Tang, Dai-Wen Pang, Gary Wong, Jianjun Chen, Xiangguo Qiu, George F Gao, Wenjun Liu, Yuhai Bi, Zhi-Ling Zhang
Ebola virus (EBOV) disease is a complex zoonosis that is highly virulent in humans, and has caused many deaths. Highly sensitive detection of EBOV is of great importance to early-stage diagnosis for increasing the probability of survival. Herein, we established a cellular beacon-mediated counting strategy for ultrasensitive EBOV assay on a micromagnetic platform. The detection platform that was assisted by on-demand magnetic field manipulation showed high integration, and enhanced complex sample pretreatment ability by magnetophoretic separation and continuous flow washing...
May 24, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29792263/an-update-on-the-use-of-immunohistochemistry-and-molecular-pathology-in-the-diagnosis-of-pre-invasive-and-malignant-lesions-in-gynecological-oncology
#11
REVIEW
Emmanouil Kalampokas, Fiona Payne, Mahalakshmi Gurumurthy
OBJECTIVE: One of the most common challenges in everyday clinical practice of gynecological oncology is to identify the type and the primary origin of a tumor. This is a crucial step in the management, treatment, prognosis, and survival of patients suffering from a gynecological malignancy. Immunohistochemistry has been widely adopted over the last three decades in pathology laboratories all over the world. Recent advances in our understanding of the differentiation of gynecological tumors based on immunohistochemical expression have resulted in use of immunohistochemistry as a major diagnostic tool in gynecology, for precise tumor classification...
May 21, 2018: Gynecologic Oncology
https://www.readbyqxmd.com/read/29791694/filling-open-screw-holes-in-the-area-of-metaphyseal-comminution-does-not-affect-fatigue-life-of-the-synthes-variable-angle-distal-femoral-locking-plate-in-the-ao-ota-33-a3-fracture-model
#12
Luis Grau, Kevin Collon, Ali Alhandi, David Kaimrajh, Maria Varon, Loren Latta, Fernando Vilella
INTRODUCTION: The aim of this study is to evaluate the biomechanical effect of filling locking variable angle (VA) screw holes at the area of metaphyseal fracture comminution in a Sawbones® (Sawbones USA, Vashon, Washington) model (AO/OTA 33A-3 fracture) using a Synthes VA locking compression plate (LCP) (Depuy Synthes, Warsaw, Indiana). MATERIALS AND METHODS: Seven Sawbones® femur models had a Synthes VA-LCP placed as indicated by the manufacturers technique...
June 1, 2018: Surgical Technology International
https://www.readbyqxmd.com/read/29791633/elderly-and-caregiver-demand-proposal-for-a-care-need-classification
#13
Daniella Pires Nunes, Tábatta Renata Pereira de Brito, Ligiana Pires Corona, Tiago da Silva Alexandre, Yeda Aparecida de Oliveira Duarte
OBJECTIVE: To propose a care need classification for elderly people by identifying their functional demands. METHOD: Cross-sectional study carried out in São Paulo, in 2006, with 1,413 elderly (≥ 60 years old), participants in the Health, Well-being and Aging study (SABE - Saúde, Bem Estar e Envelhecimento). For the care need classification, we used the Guttman Scaling method e the frequency of assistance required by the elderly. RESULTS: The hierarchy of activities of daily living had good internal consistency (α = 0...
2018: Revista Brasileira de Enfermagem
https://www.readbyqxmd.com/read/29791604/translation-and-cultural-adaptation-of-the-stroke-impact-scale-2-0-sis-a-quality-of-life-scale-for-stroke
#14
Aline Dias Brandão, Natasha Bertocco Teixeira, Maria Claudia Brandão, Milena Carlos Vidotto, José Roberto Jardim, Mariana Rodrigues Gazzotti
BACKGROUND: No specific quality-of-life scale for stroke patients has previously been translated and evaluated for reproducibility, for use in the Portuguese language. Internationally, the instrument for this purpose is the Stroke Impact Scale 2.0 (SIS). Use of of SIS enables comprehensive analysis on the impact of mild and moderate stroke on patients' lives. The aims here were to translate SIS into Portuguese, adapt it culturally, evaluate its reproducibility and correlate it with SF-36 among stroke patients...
March 2018: São Paulo Medical Journal, Revista Paulista de Medicina
https://www.readbyqxmd.com/read/29791517/costs-of-cold-acclimation-on-survival-and-reproductive-behavior-in-drosophila-melanogaster
#15
Elizabeth R Everman, Jennifer L Delzeit, F Kate Hunter, Jennifer M Gleason, Theodore J Morgan
Fitness is determined by the ability of an organism to both survive and reproduce; however, the mechanisms that lead to increased survival may not have the same effect on reproductive success. We used nineteen natural Drosophila melanogaster genotypes from the Drosophila Genetic Reference Panel to determine if adaptive plasticity following short-term acclimation through rapid cold-hardening (RCH) affects mating behavior and mating success. We confirmed that exposure to the acclimation temperature is beneficial to survival following cold stress; however, we found that this same acclimation temperature exposure led to less efficient male courtship and a significant decrease in the likelihood of mating...
2018: PloS One
https://www.readbyqxmd.com/read/29791507/a-novel-mouse-model-of-campylobacter-jejuni-enteropathy-and-diarrhea
#16
Natasa Giallourou, Gregory L Medlock, David T Bolick, Pedro Hqs Medeiros, Solanka E Ledwaba, Glynis L Kolling, Kenneth Tung, Patricia Guerry, Jonathan R Swann, Richard L Guerrant
Campylobacter infections are among the leading bacterial causes of diarrhea and of 'environmental enteropathy' (EE) and growth failure worldwide. However, the lack of an inexpensive small animal model of enteric disease with Campylobacter has been a major limitation for understanding its pathogenesis, interventions or vaccine development. We describe a robust standard mouse model that can exhibit reproducible bloody diarrhea or growth failure, depending on the zinc or protein deficient diet and on antibiotic alteration of normal microbiota prior to infection...
May 23, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29791447/development-of-an-rp-uhplc-pda-method-for-quantification-of-free-gossypol-in-cottonseed-cake-and-fungal-treated-cottonseed-cake
#17
Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio de Aquino Ribeiro, Felix Gonçalves de Siqueira, Robert Neil Gerard Miller, Simone Mendonça
Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated...
2018: PloS One
https://www.readbyqxmd.com/read/29791443/traceability-reproducibility-and-wiki-exploration-for-%C3%A3-la-carte-reconstructions-of-genome-scale-metabolic-models
#18
Méziane Aite, Marie Chevallier, Clémence Frioux, Camille Trottier, Jeanne Got, María Paz Cortés, Sebastián N Mendoza, Grégory Carrier, Olivier Dameron, Nicolas Guillaudeux, Mauricio Latorre, Nicolás Loira, Gabriel V Markov, Alejandro Maass, Anne Siegel
Genome-scale metabolic models have become the tool of choice for the global analysis of microorganism metabolism, and their reconstruction has attained high standards of quality and reliability. Improvements in this area have been accompanied by the development of some major platforms and databases, and an explosion of individual bioinformatics methods. Consequently, many recent models result from "à la carte" pipelines, combining the use of platforms, individual tools and biological expertise to enhance the quality of the reconstruction...
May 23, 2018: PLoS Computational Biology
https://www.readbyqxmd.com/read/29791430/ex-vivos-models-to-teaching-therapeutic-endoscopic-ultrasound-t-eus
#19
Everson L A Artifon, Spencer Cheng, Thaisa Nakadomari, Leandro Kashiwagi, Jose Celso Ardengh, Emilio Belmonte, Jose P Otoch
BACKGROUND: Endoscopic ultrasound training has a learning curve greater than the other endoscopic therapeutic techniques. One of the preclinical teaching methods is the use of ex vivo porcine models. AIM: To describe five ex vivo porcine models for training in therapeutic echoendoscopic procedures. MATERIALS AND METHODS: Using porcine digestive tract containing esophagus, stomach, duodenum, spleen, liver and gallbladder, five models for therapeutic echoendoscopy training were described...
January 2018: Revista de Gastroenterología del Perú: órgano Oficial de la Sociedad de Gastroenterología del Perú
https://www.readbyqxmd.com/read/29791384/copying-of-holograms-by-spot-scanning-approach
#20
Makoto Okui, Koki Wakunami, Ryutaro Oi, Yasuyuki Ichihashi, Boaz Jessie Jackin, Kenji Yamamoto
To replicate holograms, contact copying has conventionally been used. In this approach, a photosensitive material is fixed together with a master hologram and illuminated with a coherent beam. This method is simple and enables high-quality copies; however, it requires a large optical setup for large-area holograms. In this paper, we present a new method of replicating holograms that uses a relatively compact optical system even for the replication of large holograms. A small laser spot that irradiates only part of the hologram is used to reproduce the hologram by scanning the spot over the whole area of the hologram...
May 20, 2018: Applied Optics
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