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https://www.readbyqxmd.com/read/28335524/roles-of-cdk-and-ddk-in-genome-duplication-and-maintenance-meiotic-singularities
#1
REVIEW
Blanca Gómez-Escoda, Pei-Yun Jenny Wu
Cells reproduce using two types of divisions: mitosis, which generates two daughter cells each with the same genomic content as the mother cell, and meiosis, which reduces the number of chromosomes of the parent cell by half and gives rise to four gametes. The mechanisms that promote the proper progression of the mitotic and meiotic cycles are highly conserved and controlled. They require the activities of two types of serine-threonine kinases, the cyclin-dependent kinases (CDKs) and the Dbf4-dependent kinase (DDK)...
March 20, 2017: Genes
https://www.readbyqxmd.com/read/28335503/synthesis-of-randomly-substituted-anionic-cyclodextrins-in-ball-milling
#2
László Jicsinszky, Marina Caporaso, Emanuela Calcio Gaudino, Cristina Giovannoli, Giancarlo Cravotto
A number of influencing factors mean that the random substitution of cyclodextrins (CD) in solution is difficult to reproduce. Reaction assembly in mechanochemistry reduces the number of these factors. However, lack of water can improve the reaction outcomes by minimizing the reagent's hydrolysis. High-energy ball milling is an efficient, green and simple method for one-step reactions and usually reduces degradation and byproduct formation. Anionic CD derivatives have successfully been synthesized in the solid state, using a planetary ball mill...
March 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28335479/phakellistatins-an-underwater-unsolved-puzzle
#3
REVIEW
Alessandra Meli, Consiglia Tedesco, Giorgio Della Sala, Rosaria Schettini, Fernando Albericio, Francesco De Riccardis, Irene Izzo
A critical summary on the discovery of the nineteen members of the phakellistatin family (phakellistatin 1-19), cytotoxic proline-rich cyclopeptides of marine origin, is reported. Isolation, structural elucidation, and biological properties of the various-sized natural macrocycles are described, along with the total syntheses and the enigmatic issues of the cytotoxic activity reproducibility.
March 18, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28335433/evaluation-and-adaptation-of-a-laboratory-based-cdna-library-preparation-protocol-for-retrospective-sequencing-of-archived-micrornas-from-up-to-35-year-old-clinical-ffpe-specimens
#4
Olivier Loudig, Tao Wang, Kenny Ye, Juan Lin, Yihong Wang, Andrew Ramnauth, Christina Liu, Azadeh Stark, Dhananjay Chitale, Robert Greenlee, Deborah Multerer, Stacey Honda, Yihe Daida, Heather Spencer Feigelson, Andrew Glass, Fergus J Couch, Thomas Rohan, Iddo Z Ben-Dov
Formalin-fixed paraffin-embedded (FFPE) specimens, when used in conjunction with patient clinical data history, represent an invaluable resource for molecular studies of cancer. Even though nucleic acids extracted from archived FFPE tissues are degraded, their molecular analysis has become possible. In this study, we optimized a laboratory-based next-generation sequencing barcoded cDNA library preparation protocol for analysis of small RNAs recovered from archived FFPE tissues. Using matched fresh and FFPE specimens, we evaluated the robustness and reproducibility of our optimized approach, as well as its applicability to archived clinical specimens stored for up to 35 years...
March 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28335348/electrochemical-synthesis-of-polypyrrole-reduced-graphene-oxide-and-gold-nanoparticles-composite-and-its-application-to-hydrogen-peroxide-biosensor
#5
Baoyan Wu, Na Zhao, Shihua Hou, Cong Zhang
Here we report a facile eco-friendly one-step electrochemical approach for the fabrication of a polypyrrole (PPy), reduced graphene oxide (RGO), and gold nanoparticles (nanoAu) biocomposite on a glassy carbon electrode (GCE). The electrochemical behaviors of PPy-RGO-nanoAu and its application to electrochemical detection of hydrogen peroxide were investigated by cyclic voltammetry. Graphene oxide and pyrrole monomer were first mixed and casted on the surface of a cleaned GCE. After an electrochemical processing consisting of the electrooxidation of pyrrole monomer and simultaneous electroreduction of graphene oxide and auric ions (Au(3+)) in aqueous solution, a PPy-RGO-nanoAu biocomposite was synthesized on GCE...
November 21, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335313/triboelectric-hydrogen-gas-sensor-with-pd-functionalized-surface
#6
Sung-Ho Shin, Yang Hyeog Kwon, Young-Hwan Kim, Joo-Yun Jung, Junghyo Nah
Palladium (Pd)-based hydrogen (H₂) gas sensors have been widely investigated thanks to its fast reaction and high sensitivity to hydrogen. Various sensing mechanisms have been adopted for H₂ gas sensors; however, all the sensors must be powered through an external battery. We report here an H₂ gas sensor that can detect H₂ by measuring the output voltages generated during contact electrification between two friction surfaces. When the H₂ sensor, composed of Pd-coated ITO (indium tin oxide) and PET (polyethylene Terephthalate) film, is exposed to H₂, its output voltage is varied in proportion to H₂ concentration because the work function (WF) of Pd-coated surface changes, altering triboelectric charging behavior...
October 14, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335305/simultaneous-detection-of-dopamine-and-uric-acid-using-a-poly-l-lysine-graphene-oxide-modified-electrode
#7
Yuehua Zhang, Wu Lei, Yujuan Xu, Xifeng Xia, Qingli Hao
A novel, simple and selective electrochemical method was investigated for the simultaneous detection of dopamine (DA) and uric acid (UA) on a poly(l-lysine)/graphene oxide (GO) modified glassy carbon electrode (PLL/GO/GCE) by differential pulse voltammetry (DPV). The electrochemically prepared PLL/GO sensory platform toward the oxidation of UA and DA exhibited several advantages, including high effective surface area, more active sites and enhanced electrochemical activity. Compared to the PLL-modified GCE (PLL/GCE), GO-modified GCE and bare GCE, the PLL/GO/GCE exhibited an increase in the anodic potential difference and a remarkable enhancement in the current responses for both UA and DA...
September 28, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335243/correlation-between-cdse-qd-synthesis-post-synthetic-treatment-and-bhj-hybrid-solar-cell-performance
#8
Michael Eck, Michael Krueger
In this publication we show that the procedure to synthesize nanocrystals and the post-synthetic nanocrystal ligand sphere treatment have a great influence not only on the immediate performance of hybrid bulk heterojunction solar cells, but also on their thermal, long-term, and air stability. We herein demonstrate this for the particular case of spherical CdSe nanocrystals, post-synthetically treated with a hexanoic acid based treatment. We observe an influence from the duration of this post-synthetic treatment on the nanocrystal ligand sphere size, and also on the solar cell performance...
June 14, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28334930/rcas-an-rna-centric-annotation-system-for-transcriptome-wide-regions-of-interest
#9
Bora Uyar, Dilmurat Yusuf, Ricardo Wurmus, Nikolaus Rajewsky, Uwe Ohler, Altuna Akalin
In the field of RNA, the technologies for studying the transcriptome have created a tremendous potential for deciphering the puzzles of the RNA biology. Along with the excitement, the unprecedented volume of RNA related omics data is creating great challenges in bioinformatics analyses. Here, we present the RNA Centric Annotation System (RCAS), an R package, which is designed to ease the process of creating gene-centric annotations and analysis for the genomic regions of interest obtained from various RNA-based omics technologies...
February 21, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28334918/adaptive-human-immunity-drives-remyelination-in-a-mouse-model-of-demyelination
#10
Mohamed El Behi, Charles Sanson, Corinne Bachelin, Léna Guillot-Noël, Jennifer Fransson, Bruno Stankoff, Elisabeth Maillart, Nadège Sarrazin, Vincent Guillemot, Hervé Abdi, Isabelle Cournu-Rebeix, Bertrand Fontaine, Violetta Zujovic
One major challenge in multiple sclerosis is to understand the cellular and molecular mechanisms leading to disease severity progression. The recently demonstrated correlation between disease severity and remyelination emphasizes the importance of identifying factors leading to a favourable outcome. Why remyelination fails or succeeds in multiple sclerosis patients remains largely unknown, mainly because remyelination has never been studied within a humanized pathological context that would recapitulate major events in plaque formation such as infiltration of inflammatory cells...
February 22, 2017: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/28334822/posigene-automated-and-easy-to-use-pipeline-for-genome-wide-detection-of-positively-selected-genes
#11
Arne Sahm, Martin Bens, Matthias Platzer, Karol Szafranski
Many comparative genomics studies aim to find the genetic basis of species-specific phenotypic traits. A prevailing strategy is to search genome-wide for genes that evolved under positive selection based on the non-synonymous to synonymous substitution ratio. However, incongruent results largely due to high false positive rates indicate the need for standardization of quality criteria and software tools. Main challenges are the ortholog and isoform assignment, the high sensitivity of the statistical models to alignment errors and the imperative to parallelize large parts of the software...
March 15, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28334648/optimizing-withdrawal-from-drinking-water-reservoirs-to-reduce-downstream-temperature-pollution-and-reservoir-hypoxia
#12
M Weber, K Rinke, M R Hipsey, B Boehrer
Sustainable management of drinking water reservoirs requires balancing the demands of water supply whilst minimizing environmental impact. This study numerically simulates the effect of an improved withdrawal scheme designed to alleviate the temperature pollution downstream of a reservoir. The aim was to identify an optimal withdrawal strategy such that water of a desirable discharge temperature can be supplied downstream without leading to unacceptably low oxygen concentrations within the reservoir. First, we calibrated a one-dimensional numerical model for hydrodynamics and oxygen dynamics (GLM-AED2), verifying that the model reproduced water temperatures and hypolimnetic dissolved oxygen concentrations accurately over a 5 year period...
March 20, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28334612/computing-by-robust-transience-how-the-fronto-parietal-network-performs-sequential-category-based-decisions
#13
Warasinee Chaisangmongkon, Sruthi K Swaminathan, David J Freedman, Xiao-Jing Wang
Decision making involves dynamic interplay between internal judgements and external perception, which has been investigated in delayed match-to-category (DMC) experiments. Our analysis of neural recordings shows that, during DMC tasks, LIP and PFC neurons demonstrate mixed, time-varying, and heterogeneous selectivity, but previous theoretical work has not established the link between these neural characteristics and population-level computations. We trained a recurrent network model to perform DMC tasks and found that the model can remarkably reproduce key features of neuronal selectivity at the single-neuron and population levels...
March 22, 2017: Neuron
https://www.readbyqxmd.com/read/28334172/evaluation-of-neoseiulus-barkeri-acari-phytoseiidae-for-control-of-eotetranychus-kankitus-acari-tetranychidae
#14
Ya-Ying Li, Ming-Xiu Liu, Hai-Wei Zhou, Chuan-Bei Tian, Guo-Hao Zhang, Yi-Qing Liu, Huai Liu, Jin-Jun Wang
Eotetranychus kankitus Ehara (Acari: Tetranychidae) is an important pest in Chinese citrus orchards. In the present study, we aimed to evaluate the potential of Neoseiulus barkeri (Hughes) (Acari: Phytoseiidae) for the biological control of E. kankitus. A two-sex life table of E. kankitus and N. barkeri was constructed to estimate development and fecundity. The functional response and stage-specific predation rate were analyzed to evaluate predation capacity. In addition, a timing model was used to project populations of E...
March 3, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28334000/temperature-and-food-quantity-effects-on-the-harpacticoid-copepod-nitocra-spinipes-combining-in-vivo-bioassays-with-population-modeling
#15
Josef Koch, Thuy T Bui, Elin Lundström Belleza, Markus Brinkmann, Henner Hollert, Magnus Breitholtz
The harpacticoid copepod Nitocra spinipes has become a popular model species for toxicity testing over the past few decades. However, the combined influence of temperature and food shortage, two climate change-related stressors, has never been assessed in this species. Consequently, effects of three temperatures (15, 20 and 25°C) and six food regimes (between 0 and 5 × 105 algal cells/mL) on the life cycle of N. spinipes were examined in this study. Similarly to other copepod species, development times and brood sizes decreased with rising temperatures...
2017: PloS One
https://www.readbyqxmd.com/read/28333955/producing-or-reproducing-reasoning-socratic-dialog-is-very-effective-but-only-for-a-few
#16
Andrea Paula Goldin, Olivia Pedroncini, Mariano Sigman
Successful communication between a teacher and a student is at the core of pedagogy. A well known example of a pedagogical dialog is 'Meno', a socratic lesson of geometry in which a student learns (or 'discovers') how to double the area of a given square 'in essence, a demonstration of Pythagoras' theorem. In previous studies we found that after engaging in the dialog participants can be divided in two kinds: those who can only apply a rule to solve the problem presented in the dialog and those who can go beyond and generalize that knowledge to solve any square problems...
2017: PloS One
https://www.readbyqxmd.com/read/28333901/influence-of-the-recurrent-syncope-episodes-on-neurocognitive-functions-in-patients-with-vasovagal-syncope
#17
Joanna Jędrzejczyk-Spaho, Artur Z Pietrucha, Agnieszka Borowiec, Irena Bzukała, Mateusz Wnuk, Ewa Konduracka, Jadwiga Nessler
The aim of study was to evaluate an influence of recurrent syncope episodes on the neurocognitive functions (NCF) in patients with suspected VVS. AIM: The aim of study was to evaluate an influence of recurrent syncope episodes on the neurocognitive functions (NCF) in patients with suspected VVS. MATERIALS AND METHODS: Study population: 24 pts. (16 women) aged 17-70 yrs (mean age 40 years), with suspected VVS, referred to HUTT (head-up tilt test). All pts. underwent initial evaluation regarding to the number and circumstances of the syncopal and/or presyncopal spells...
March 21, 2017: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/28333831/deployed-skills-training-for-whole-blood-collection-by-a-special-operations-expeditionary-surgical-team
#18
Linda C Benavides, Iain M Smith, Jerome M Benavides, Douglas M Bowley, Heidi A Doughty, Jonathan B Lundy
BACKGROUND: Non-compressible hemorrhage is the leading cause of potentially preventable battlefield death. Combining casualty retrieval from the battlefield and damage control resuscitation (DCR) within the "golden hour" increases survival. However, transfusion requirements may exceed the current blood component stocks held by forward surgical teams. Warm fresh whole blood (WFWB) is an alternative. We report WFWB transfusion training developed by and delivered to a US Golden Hour Offset Surgical Treatment Team (GHOST-T) and the resulting improvement in confidence with WFWB transfusion...
March 22, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28333782/the-normal-adult-human-internal-auditory-canal-a-volumetric-multidetector-computed-tomography-study
#19
Fahad Essbaiheen, Tarek Hegazi, Lorne Rosenbloom
OBJECTIVE: The purpose of this study was to demonstrate that volumetric analysis of multidetector computed tomography (CT) images can be used to calculate the volume of the adult human internal auditory canal (IAC) reproducibly, and to describe the range of normal IAC volumes in the adult population with subgroup analysis of sex, age, and laterality. BACKGROUND: Previous studies of the IAC have typically used measurements in two dimensions or by using casts of cadavers to measure IAC volumes...
March 22, 2017: Otology & Neurotology
https://www.readbyqxmd.com/read/28333456/calixsmaragdyrin-a-versatile-ligand-for-coordination-complexes
#20
Tamal Chatterjee, Brian Molnar, G G Theophall, Way-Zen Lee, K V Lakshmi, Mangalampalli Ravikanth
The Ru(II) and BF2 complexes of calixsmaragdyrin were prepared under simple reaction conditions and characterized by HR-MS, 1D and 2D NMR spectroscopy, optical spectroscopy, and electrochemistry, and the structure of the Ru(II) complex of calixsmaragdyrin was elucidated by X-ray crystallography. The crystal structure of the Ru(II) complex revealed that the Ru(II) ion is hexacoordinate with the three pyrrole nitrogen ligands from the tripyrrin unit of the calixsmaragdyrin macrocycle, and the remaining coordination sites of Ru(II) ion were occupied by two carbonyl groups and one hydroxyl (-OH) group...
March 23, 2017: Inorganic Chemistry
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