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https://www.readbyqxmd.com/read/28651288/development-of-a-high-performance-liquid-chromatographic-method-for-asiaticoside-quantification-in-different-skin-layers-after-topical-application-of-a-centella-asiatica-extract
#1
Priscila Bianca Rodrigues da Rocha, Bruno Dos Santos Souza, Lígia Marquez Andrade, Ricardo Neves Marreto, Eliana Martins Lima, Stephânia Fleury Taveira
The topical application of Centella asiatica extract has been commonly used for many different purposes but especially for cosmetic use in the treatment of gynoid lipodystrophy. Asiaticoside, the most active component in this extract, is responsible for its therapeutic activities. However, little is known to date about asiaticoside skin penetration. Thus, an analytical method for asiaticoside quantification in different skin layers after the topical application of C. asiatica extract was developed and skin permeation studies were performed with the plant extract to apply the analytical method developed...
June 26, 2017: Planta Medica
https://www.readbyqxmd.com/read/28651177/particle-size-distribution-psd-of-pulverized-hair-a-quantitative-approach-of-milling-efficiency-and-its-correlation-with-drug-extraction-efficiency
#2
Aline Garcia da Rosa Chagas, Eliani Spinelli, Sorele Batista Fiaux, Adriana da Silva Barreto, Silvana Vianna Rodrigues
Different types of hair were submitted to different milling procedures and their resulting powders were analyzed by scanning electron microscopy (SEM) and laser diffraction (LD). SEM results were qualitative whereas LD results were quantitative and accurately characterized the hair powders through their particle size distribution (PSD). Different types of hair were submitted to an optimized milling conditions and their PSD was quite similar. A good correlation was obtained between PSD results and ketamine concentration in a hair sample analyzed by LC-MS/MS...
June 15, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28651158/prospective-validation-of-a-lymphocyte-infiltration-prognostic-test-in-stage-iii-colon-cancer-patients-treated-with-adjuvant-folfox
#3
Jean-François Emile, Catherine Julié, Karine Le Malicot, Come Lepage, Josep Tabernero, Enrico Mini, Gunnar Folprecht, Jean-Luc Van Laethem, Stéphanie Dimet, Camille Boulagnon-Rombi, Marc-Antoine Allard, Frédérique Penault-Llorca, Jaafar Bennouna, Pierre Laurent-Puig, Julien Taieb
BACKGROUND: The prognostic value of lymphocyte infiltration (LI) of colorectal carcinoma (CC) has been demonstrated by several groups. However, no validated test is currently available for clinical practice. We previously described an automated and reproducible method for testing LI and aimed to validate it for clinical use. PATIENTS AND METHODS: According to National Institutes of Health criteria, we designed a prospective validation of this biomarker in patients included in the PETACC8 phase III study...
June 23, 2017: European Journal of Cancer
https://www.readbyqxmd.com/read/28651056/significantly-improved-protein-folding-thermodynamics-using-a-dispersion-corrected-water-model-and-a-new-residue-specific-force-field
#4
Hao-Nan Wu, Fan Jiang, Yun-Dong Wu
An accurate potential energy model is crucial for biomolecular simulations. Despite many recent improvements of classical protein force fields, there are remaining key issues: much weaker temperature-dependence of folding/unfolding equilibrium, and overly collapsed unfolded or disordered states. For the later problem, a new water model (TIP4P-D) has been proposed to correct the significantly underestimated water dispersion interactions. Here, using TIP4P-D, we reveal problems in current force fields through failures in folding model systems (a polyalanine peptide, Trp-cage and the GB1 hairpin)...
June 26, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28651049/dissolvable-and-recyclable-random-lasers
#5
Xiaoyu Shi, Yu-Ming Liao, Hsia-Yu Lin, Po-Wei Tsao, Meng-Jer Wu, Shih-Yao Lin, Hsiu-Hao Hu, Zhaona Wang, Tai-Yuan Lin, Ying-Chih Lai, Yang-Fang Chen
An integrated random laser based on green materials with dissolubility and recyclability is created and demonstrated. The dissolvable and recyclable random laser (DRRL) can be dissolved in water accompanying with the decay of emission intensity and the increment in lasing threshold. Furthermore, the DRRL can be reused after the process of de-ionized treatment, exhibiting excellent reproducibility with several recycling processes.
June 26, 2017: ACS Nano
https://www.readbyqxmd.com/read/28651045/photoinduced-charge-transfer-versus-fragmentation-pathways-in-lanthanum-cyclopentadienyl-complexes
#6
Yulun Han, Qingguo Meng, Bakhtiyor Rasulev, P Stanley May, Mary T Berry, Dmitri S Kilin
This study compares two competing pathways of photoexcitations in gas-phase metal-organic complexes: first, a sequence of phonon-assisted electronic transitions leading to dissipation of the energy of photoexcitations and second, a sequence of light-driven electronic transitions leading to photolysis. Phonon-assisted charge carrier dynamics is investigated by combination of the density matrix formalism and on-the-fly nonadiabatic couplings. Light-driven fragmentation is modeled by a time-dependent excited-state molecular dynamics (TDESMD) algorithm based on Rabi theory and principles similar to the trajectory surface hopping approximation...
June 26, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28651008/production-and-purification-of-human-hsp90%C3%AE-in-escherichia-coli
#7
Martina Radli, Dmitry B Veprintsev, Stefan G D Rüdiger
The molecular chaperone Hsp90 is an essential member of the cellular proteostasis system. It plays an important role in the stabilisation and activation of a large number of client proteins and is involved in fatal disease processes, e.g. Alzheimer disease, cancer and cystic fibrosis. This makes Hsp90 a crucial protein to study. Mechanistic studies require large amounts of protein but the production and purification of recombinant human Hsp90 in Escherichia coli is challenging and laborious. Here we identified conditions that influence Hsp90 production, and optimised a fast and efficient purification protocol...
2017: PloS One
https://www.readbyqxmd.com/read/28650903/thigh-ultrasound-monitoring-identifies-decreases-in-quadriceps-femoris-thickness-as-a-frequent-observation-in-critically-ill-children
#8
Frederic V Valla, David K Young, Muriel Rabilloud, Uvaraj Periasami, Manoj John, Florent Baudin, Carole Vuillerot, Aurélie Portefaix, Deborah White, Jenna A Ridout, Rosan Meyer, Bénédicte Gaillard Le Roux, Etienne Javouhey, Nazima Pathan
OBJECTIVES: Significant muscle wasting develops in critically ill adults, with subsequent worse outcomes. In the pediatric setting, occurrence and effects of muscle wasting are undescribed; this is in part due to a lack of validated, objective methods for assessing muscle wasting. A single measurement of quadriceps femoris thickness has failed to show consistent reproducibility. We hypothesized that averaging repeated measurements could afford good reproducibility to allow for quadriceps femoris thickness decline detection and monitoring...
June 23, 2017: Pediatric Critical Care Medicine
https://www.readbyqxmd.com/read/28650900/a-high-throughput-hplc-ms-ms-assay-for-the-detection-quantification-and-simultaneous-structural-confirmation-of-136-drugs-and-metabolites-in-human-urine
#9
Jacek Klepacki, Baharak Davari, Marion Boulet, Ryan Lizarraga, Uwe Christians
BACKGROUND: Due to its superior sensitivity and specificity, multi-analyte high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) has become the gold standard in clinical toxicology. Although several qualitative and quantitative LC-MS/MS assays on various mass spectrometry platforms have been described in the literature, the majority of these methods either analyze only a limited number of compounds, and/or require tedious and time-consuming sample preparation...
June 22, 2017: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/28650654/evaluation-of-a-rapid-method-for-screening-heat-stress-tolerance-using-three-korean-wheat-triticum-aestivum-l-cultivars
#10
Hai An Truong, Chan Young Jeong, Won Je Lee, Byung Cheon Lee, Namhyun Chung, Chon-Sik Kang, Young-Keun Cheong, Suk-Whan Hong, Hojoung Lee
Thermotolerance in plants is a topic of concern given the current trends in global warming. Here, we aimed to develop a rapid and reproducible screening method for selection of heat stress- tolerant wheat varieties to expedite the breeding process. We tested the robustness of the screen in three Korean wheat cultivars, 'BackJung', 'KeumKang', and 'ChoKyeong'. We showed that 4-day-old seedlings of 'KeumKang' had the highest survival rates after a 45C treatment for 20 h. Moreover, the ability to retain chlorophyll and antioxidant activity was also highest in 'KeumKang'...
June 26, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28650590/intra-and-interobserver-reproducibility-of-bruch-s-membrane-opening-minimum-rim-width-measurements-with-spectral-domain-optical-coherence-tomography
#11
Alexandre S C Reis, Camila E S Zangalli, Ricardo Y Abe, André L Silva, Jayme R Vianna, José Paulo C Vasconcellos, Vital P Costa
PURPOSE: To investigate the reproducibility of Bruch's membrane opening minimum rim width (BMO-MRW) and retinal nerve fibre layer thickness (RNFLT) measurements using spectral domain optical coherence tomography (SD-OCT). Additionally, to investigate the reproducibility of BMO area measurements and fovea to BMO centre (FoBMO) angle. METHODS: Participants were healthy subjects (n = 30) and patients with glaucoma (n = 26). One eye of each participant was scanned to obtain optic nerve head (24 radial B-scans) and peripapillary (one circular B-scan) images by three independent examiners...
June 26, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28650393/orthotopic-patient-derived-pancreatic-cancer-xenografts-engraft-into-the-pancreatic-parenchyma-metastasize-and-induce-muscle-wasting-to-recapitulate-the-human-disease
#12
Kristina L Go, Daniel Delitto, Sarah M Judge, Michael H Gerber, Thomas J George, Kevin E Behrns, Steven J Hughes, Andrew R Judge, Jose G Trevino
OBJECTIVE: Limitations associated with current animal models serve as a major obstacle to reliable preclinical evaluation of therapies in pancreatic cancer (PC). In an effort to develop more reliable preclinical models, we have recently established a subcutaneous patient-derived xenograft (PDX) model. However, critical aspects of PC responsible for its highly lethal nature, such as the development of distant metastasis and cancer cachexia, remain underrepresented in the flank PDX model...
June 22, 2017: Pancreas
https://www.readbyqxmd.com/read/28650171/comprehensive-lipoprotein-characterization-using-lipidomics-analysis-of-human-plasma
#13
Nicolas Christinat, Mojgan Masoodi
Lipoproteins are responsible for the transport of lipids and other nutrients in the circulation and therefore play an important role in lipid metabolism and dyslipidemia. They have also been linked to multiple metabolic disorders including cardiovascular disease, thus understanding their lipid composition is of crucial importance. Characterization of lipoproteins is a challenging task due to their heterogeneity. In particular, their fractionation is often laborious and time-consuming, making large sets of clinical samples difficult to analyze...
June 26, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28650151/orthogonal-comparison-of-gc-ms-and-1-h-nmr-spectroscopy-for-short-chain-fatty-acid-quantitation
#14
Jingwei Cai, Jingtao Zhang, Yuan Tian, Limin Zhang, Emmanuel Hatzakis, Kristopher W Krausz, Philip B Smith, Frank J Gonzalez, Andrew D Patterson
Short chain fatty acids (SCFAs) are important regulators of host physiology and metabolism and may contribute to obesity and associated metabolic diseases. Interest in SCFAs has increased in part due to the recognized importance of how production of SCFAs by the microbiota may signal to the host. Therefore, reliable, reproducible, and affordable methods for SCFA profiling are required for accurate identification and quantitation. In the current study, four different methods for SCFA (acetic acid, propionic acid, and butyric acid) extraction and quantitation were compared using two independent platforms including gas chromatography coupled with mass spectrometry (GC-MS) and (1)H nuclear magnetic resonance (NMR) spectroscopy...
June 26, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28650110/reproducibility-of-in-vivo-magnetic-resonance-imaging-t1-rho-and-t2-relaxation-time-measurements-of-hip-cartilage-at-3-0t-in-healthy-volunteers
#15
Angeline Nemeth, Lucy Di Marco, Florent Boutitie, Michael Sdika, Denis Grenier, Muriel Rabilloud, Olivier Beuf, Jean-Baptiste Pialat
PURPOSE: To assess the T1 ρ and T2 values in the hip cartilage of healthy volunteers and to evaluate the reproducibility of these measurements. MATERIALS AND METHODS: The right hip joint of 30 asymptomatic volunteers was explored with 3T magnetic resonance imaging (MRI). Quantitative 3D T1 ρ- and T2 -maps sequences were repeated twice with a 30-minute delay (immediate reproducibility). The same protocol was repeated 14 days later (short-term reproducibility). Immediate and short-term reproducibility were estimated using coefficients of variation and correlation concordance coefficients (CCC)...
June 26, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28650041/competition-between-the-h-and-d-atom-transfer-channels-in-the-h2o-hd-reaction-reduced-dimensional-quantum-and-quasi-classical-studies
#16
Hongwei Song, Anyang Li, Minghui Yang, Hua Guo
The ion-molecule reaction between a water cation and a hydrogen molecule has recently attracted considerable interest due to its importance in astrochemistry. In this work, the intramolecular isotope effect of the H2O(+) + HD reaction is investigated using a seven-dimensional initial state-selected time-dependent wave packet approach as well as a full-dimensional quasi-classical trajectory method on a full-dimensional ab initio global potential energy surface. The calculated branching ratios for the formation of H3O(+) and H2DO(+)via H- and D-transfer agree reasonably well with the experimental values...
June 26, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28649960/gold-nanoparticles-decorated-electrospun-poly-n-vinyl-2-pyrrolidone-nanofibers-with-tunable-size-and-coverage-density-for-nanomolar-detection-of-single-and-binary-component-dyes-by-surface-enhanced-raman-spectroscopy
#17
Alfin Kurniawan, Meng-Jiy Wang
The application of the electrospun nanomaterials to surface-enhanced Raman spectroscopy (SERS) is a rapidly evolving field which holds potential for future developments in the generation of portable plasmonic-based detection platforms. In this study, a simple approach to fabricate electrospun poly(N-vinylpyrrolidone) (PVP) mats decorated with gold nanoparticles (AuNPs) by combining electrospinning and calcination was presented. AuNPs were decorated on the fiber mat surface through electrostatic interactions between positively charged aminosilane groups and negatively charged AuNPs...
June 26, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28649712/cross-platform-compatibility-of-de-novo-aligned-snps-in-a-non-model-butterfly-genus
#18
Erin O Campbell, Corey S Davis, Julian R Dupuis, Kevin Muirhead, Felix A H Sperling
High-throughput sequencing methods for genotyping genome-wide markers are being rapidly adopted for phylogenetics of non-model organisms in conservation and biodiversity studies. However, the reproducibility of SNP genotyping and degree of marker overlap or compatibility between datasets from different methodologies have not been tested in non-model systems. Using double-digest restriction site associated DNA sequencing, we sequenced a common set of 22 specimens from the butterfly genus Speyeria on two different Illumina platforms, using two variations of library preparation...
June 26, 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/28649594/specific-macronutrients-exert-unique-influences-on-the-adipose-liver-axis-to-promote-hepatic-steatosis-in-mice
#19
Caroline C Duwaerts, Amin M Amin, Kevin Siao, Chris Her, Mark Fitch, Carine Beysen, Scott M Turner, Amanda Goodsell, Jody L Baron, James P Grenert, Soo-Jin Cho, Jacquelyn J Maher
BACKGROUND & AIMS: The factors that distinguish metabolically healthy obesity from metabolically unhealthy obesity are not well understood. Diet has been implicated as a determinant of the unhealthy obesity phenotype, but which aspects of the diet induce dysmetabolism are unknown. The goal of this study was to investigate whether specific macronutrients or macronutrient combinations provoke dysmetabolism in the context of isocaloric, high-energy diets. METHODS: Mice were fed 4 high-energy diets identical in calorie and nutrient content but different in nutrient composition for 3 weeks to 6 months...
September 2017: Cellular and Molecular Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28649432/an-individual-based-model-for-collective-cancer-cell-migration-explains-speed-dynamics-and-phenotype-variability-in-response-to-growth-factors
#20
Damian Stichel, Alistair M Middleton, Benedikt F Müller, Sofia Depner, Ursula Klingmüller, Kai Breuhahn, Franziska Matthäus
Collective cell migration is a common phenotype in epithelial cancers, which is associated with tumor cell metastasis and poor patient survival. However, the interplay between physiologically relevant pro-migratory stimuli and the underlying mechanical cell-cell interactions are poorly understood. We investigated the migratory behavior of different collectively migrating non-small cell lung cancer cell lines in response to motogenic growth factors (e.g. epidermal growth factor) or clinically relevant small compound inhibitors...
2017: NPJ Systems Biology and Applications
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