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https://www.readbyqxmd.com/read/28434198/eukaryotic-diversity-in-late-pleistocene-marine-sediments-around-a-shallow-methane-hydrate-deposit-in-the-japan-sea
#1
M Kouduka, A S Tanabe, S Yamamoto, K Yanagawa, Y Nakamura, F Akiba, H Tomaru, H Toju, Y Suzuki
Marine sediments contain eukaryotic DNA deposited from overlying water columns. However, a large proportion of deposited eukaryotic DNA is aerobically biodegraded in shallow marine sediments. Cold seep sediments are often anaerobic near the sediment-water interface, so eukaryotic DNA in such sediments is expected to be preserved. We investigated deeply buried marine sediments in the Japan Sea, where a methane hydrate deposit is associated with cold seeps. Quantitative PCR analysis revealed the reproducible recovery of eukaryotic DNA in marine sediments at depths up to 31...
April 23, 2017: Geobiology
https://www.readbyqxmd.com/read/28434036/acromion-morphology-and-bone-mineral-density-distribution-suggest-favorable-fixation-points-for-anatomic-acromioclavicular-reconstruction
#2
Andreas Voss, Felix Dyrna, Andrea Achtnich, Alex Hoberman, Elifho Obopilwe, Andreas B Imhoff, Augustus D Mazzocca, Knut Beitzel
PURPOSE: Recent techniques for acromioclavicular (AC) joint reconstruction focus on additional AC cerclage to coracoclavicular (CC)-reconstructions. Due to the specific slim bone morphology at the acromion, there are concerns regarding these additional bone tunnels, as they may predispose to fracture and break out. The purpose of this study was to investigate anatomic properties of the acromion which may help improve surgical techniques directed at injuries to the AC joint. It was hypothesized that via measurements of thickness and density points of increased strength and support could be identified on the acromion...
April 22, 2017: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/28433990/two-dimensional-ultrasound-evaluation-of-the-fetal-cerebral-aqueduct-improving-the-antenatal-diagnosis-and-counseling-of-aqueductal-stenosis
#3
Fernando Viñals, Pilar Ruiz, Gabriel Quiroz, Francisco A Guerra, Flavia Correa, Daniel Martínez, Bienvenido Puerto
OBJECTIVE: To describe a technique for the visualization and measurement of cerebral aqueduct diameter through a 2D sagittal median plane, and to report its aspect and measurement in fetuses with aqueductal stenosis (AS). METHODS: This was a cross-sectional study of 207 morphologically normal fetuses in low-risk pregnancies between 20 and 36 weeks of gestation. The cerebral aqueduct was visualized transvaginally in a midsagittal plane, and measurements of its greatest diameter (ampulla) were taken independently by an expert and a nonexpert sonographer...
April 22, 2017: Fetal Diagnosis and Therapy
https://www.readbyqxmd.com/read/28433858/reduced-graphene-oxide-chitosan-aptamer-interface-as-new-platform-for-ultrasensitive-detection-of-human-epidermal-growth-factor-receptor-2
#4
Arezoo Tabasi, Abdollah Noorbakhsh, Ensiyeh Sharifi
The present work describes an ultrasensitive electrochemical aptamer-based assay for detection of human epidermal growth factor receptor 2 protein (HER2) cancer biomarker as a model analyte. Results show that the reduced graphene oxide-chitosan (rGO-Chit) film as a suitable electrode material possesses great favorable properties including high homogeneity, good stability, large surface area and high fraction of amine groups as aptamer binding sites. Various steps of aptasensor fabrication were characterized using microscopic, energy-dispersive X-ray spectroscopy (EDAX), Fourier transform infrared (FTIR) spectroscopy and electrochemical techniques...
April 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28433811/rapid-and-sensitive-detection-of-salmonid-alphavirus-using-taqman-real-time-pcr
#5
Wen Shi, Aochen Song, Shuai Gao, Yuting Wang, Lijie Tang, Yigang Xu, Tong Ren, Yijing Li, Min Liu
Salmonid alphavirus (SAV) infection has led to the spread of salmon pancreas disease (PD) and sleeping disease (SD) to salmonids in several countries in Europe, resulting in tremendous economic losses to the fish farming industry. Recently, with increases in the fish import trade, many countries in which SAV has been unreported, such as China, may be seriously threatened by these diseases. It is therefore necessary to develop efficient detection methods for the prevention and diagnosis of SAV infection. In this study, a rapid and sensitive TaqMan real-time PCR method was established and assessed for this purpose...
April 19, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28433649/chronic-nicotine-attenuates-behavioral-and-synaptic-plasticity-impairments-in-a-streptozotocin-model-of-alzheimer-s-disease
#6
Esteves Im, Lopes-Aguiar C, Rossignoli Mt, Ruggiero Rn, Broggini Acs, Bueno-Junior Ls, Kandratavicius L, Monteiro Mr, Romcy-Pereira Rn, Leite Jp
Brain glucose metabolism is altered in sporadic Alzheimer's disease (sAD), whose pathologies are reproduced in rodents by intracerebroventricular (icv) infusion of streptozotocin (STZ) in subdiabetogenic doses. The icv-STZ model also culminates in central cholinergic dysfunctions, which in turn are known to underlie both the sAD cognitive decline, and synaptic plasticity impairments. Considering the cognitive-enhancing potential of chronic nicotine (Nic), we investigated whether it attenuates icv-STZ-induced impairments in recognition memory and synaptic plasticity in a cognition-relevant substrate: the hippocampal CA1-medial prefrontal cortex (mPFC) pathway...
April 19, 2017: Neuroscience
https://www.readbyqxmd.com/read/28433605/stability-of-patient-specific-features-of-altered-dna-replication-timing-in-xenografts-of-primary-human-acute-lymphoblastic-leukemia
#7
Takayo Sasaki, Juan Carlos Rivera-Mulia, Daniel Vera, Jared Zimmerman, Sunny Das, Michelle Padget, Naoto Nakamichi, Bill H Chang, Jeff Tyner, Brian J Druker, Andrew P Weng, Curt I Civin, Connie J Eaves, David M Gilbert
Genome-wide DNA replication timing (RT) profiles reflect the global 3D chromosome architecture of cells. They also provide a comprehensive and unique megabase-scale picture of the cellular epigenetic state. Thus normal differentiation involves reproducible changes in RT and transformation generally perturbs these, although the potential effects of altered RT on the properties of transformed cells remain largely unknown. A major challenge to interrogating these issues in human acute lymphoid leukemia (ALL) is the low proliferative activity of most of the cells, which may be further reduced in cryopreserved samples and difficult to overcome in vitro...
April 19, 2017: Experimental Hematology
https://www.readbyqxmd.com/read/28433601/volumetric-versus-unidimensional-measures-of-metastatic-colorectal-cancer-in-assessing-disease-response
#8
Meghan G Lubner, Nicholas Stabo, Sam J Lubner, Alejandro Munoz Del Rio, Chihwa Song, Perry J Pickhardt
INTRODUCTION: The purpose of this study was to compare unidimensional (1D/linear) and volumetric (3D) measures of metastatic colorectal cancer (mCRC) at computed tomography (CT) for predicting clinical outcome. PATIENTS AND METHODS: Analysis of CT images in 105 patients (mean age, 59 years; range, 25-81 years; 45 women, 60 men) receiving treatment for mCRC was performed. Both unidimensional and volumetric measures were obtained on index lesions at 3 time points (baseline/midpoint/post-therapy; mean interval, 4...
March 27, 2017: Clinical Colorectal Cancer
https://www.readbyqxmd.com/read/28433580/description-of-a-novel-multiplex-avidity-assay-for-evaluating-hpv-antibodies
#9
Allison M Brady, Elizabeth R Unger, Gitika Panicker
Limited data exists regarding antibody avidity for human papillomavirus (HPV). We describe development of a multiplex electrochemiluminescent avidity ELISA for four HPV types (HPV 6, 11, 16, 18) by adding a dissociating step to our established multiplex HPV VLP ELISA. Initial experiments exploring ammonium thiocyanate, sodium thiocyanate and guanidine hydrochloride (GuHCl) as dissociating agents identified GuHCl as most promising. Dissociation conditions with GuHCl were varied (concentration, incubation time, temperature) to select conditions with minimal impact on VLP integrity as measured with monoclonal antibodies to conformational epitopes...
April 19, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28433479/modeling-short-range-substitution-order-and-disorder-in-crystals-application-to-the-ga-si-distribution-in-a-natrolite-zeolite
#10
Sylvian Cadars, Nak Ho Ahn, Kirill Okhotnikov, Jiho Shin, Aurélie Vicente, Suk Bong Hong, Christian Fernandez
Atomic substitutions are a central feature of the physicochemical properties of an increasing number of solid-state materials. The complexity that this chemical disorder locally generates in otherwise crystalline solids poses a major challenge to the understanding of the relationships between the structure and properties of materials at the atomic and molecular level. Strategies designed to efficiently explore the ensemble of local chemical environments present in disordered crystals and predict their signatures in local spectroscopies such as solid-state nuclear magnetic resonance (NMR) are therefore essential...
April 13, 2017: Solid State Nuclear Magnetic Resonance
https://www.readbyqxmd.com/read/28433391/mechanisms-of-anterior-posterior-stability-of-the-knee-joint-under-load-bearing
#11
Ryan J Reynolds, Peter S Walker, John Buza
The anterior-posterior (AP) stability of the knee is an important aspect of functional performance. Studies have shown that the stability increases when compressive loads are applied, as indicated by reduced laxity, but the mechanism has not been fully explained. A test rig was designed which applied combinations of AP shear and compressive forces, and measured the AP displacements relative to the neutral position. Five knees were evaluated at compressive loads of 0, 250, 500, and 750N, with the knee at 15° flexion...
April 7, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/28433348/the-influence-of-polyethylene-bearing-thickness-on-the-tibiofemoral-kinematics-of-a-bicruciate-retaining-total-knee-arthroplasty
#12
Geert Peersman, Josh Slane, Margo Dirckx, Arne Vandevyver, Philipp Dworschak, Thomas J Heyse, Lennart Scheys
BACKGROUND: The recently reintroduced bicruciate retaining Total Knee Arthroplasty (BCR TKA) is an effort to reproduce kinematics closer to the native knee. However, there is no data on appropriate balancing with this implant. Balancing is crucial and challenging as medial and lateral polyethylene (PE) inlays are modular, which allows for placement of different thicknesses in the medial and lateral compartments. This study aimed at providing a detailed kinematic view on balancing BCR TKA...
April 19, 2017: Knee
https://www.readbyqxmd.com/read/28433213/three-dimensional-surgical-modelling-with-an-open-source-software-protocol-study-of-precision-and-reproducibility-in-mandibular-reconstruction-with-the-fibula-free-flap
#13
L Ganry, J Quilichini, C M Bandini, P Leyder, B Hersant, J P Meningaud
Very few surgical teams currently use totally independent and free solutions to perform three-dimensional (3D) surgical modelling for osseous free flaps in reconstructive surgery. This study assessed the precision and technical reproducibility of a 3D surgical modelling protocol using free open-source software in mandibular reconstruction with fibula free flaps and surgical guides. Precision was assessed through comparisons of the 3D surgical guide to the sterilized 3D-printed guide, determining accuracy to the millimetre level...
April 19, 2017: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28433177/kinetic-study-of-thermal-degradation-of-chitosan-as-a-function-of-deacetylation-degree
#14
M A Gámiz-González, D M Correia, S Lanceros-Mendez, V Sencadas, J L Gómez Ribelles, A Vidaurre
Thermal degradation of chitosan with varying deacetylation degree (DD) ranging between 50 and 85% was analyzed by dynamic thermogravimetric analysis at different heating rates. The present study focused on the temperature range between 500 and 800K, above water evaporation. Thermal degradation showed a main degradation stage in this temperature interval with a second stage that appeared in the weight derivative curves as a shoulder in the high temperature side of the main peak with increasing intensity as the DD decreased...
July 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28433061/graphene-coated-polymeric-anion-exchangers-for-ion-chromatography
#15
Kai Zhang, Minyi Cao, Chaoyan Lou, Shuchao Wu, Peimin Zhang, Mingyu Zhi, Yan Zhu
Carbonaceous stationary phases have gained much attention for their peculiar selectivity and robustness. Herein we report the fabrication and application of a graphene-coated polymeric stationary phase for anion exchange chromatography. The graphene-coated particles were fabricated by a facile evaporation-reduction method. These hydrophilic particles were proven appropriate substrates for grafting of hyperbranched condensation polymers (HBCPs) to make pellicular anion exchangers. The new phase was characterized by zeta potentials, Fourier transform infrared spectroscopy, thermogravimetry and scanning electron microscope...
June 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28433040/predicting-miscibility-of-binary-liquids-from-small-cluster-qce-calculations
#16
Johannes Ingenmey, Michael von Domaros, Barbara Kirchner
The quantum cluster equilibrium method is applied to model binary systems of molecular solvents. We minimize the computational effort as well as the experimental input and present the results obtained for the completely miscible acetonitrile/acetone, benzene/acetone, and water/acetone systems, as well as for the hardly miscible water/benzene system. Only clusters of sizes up to n = 3 are applied and these are optimized employing the low-cost functional PBEh-3c. The thermodynamic functions of the pure liquids are in reasonable agreement with experiments...
April 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28432786/the-comprehensive-connectome-of-a-neural-substrate-for-on-motion-detection-in-drosophila
#17
Shin-Ya Takemura, Aljoscha Nern, Dmitri B Chklovskii, Louis K Scheffer, Gerald M Rubin, Ian A Meinertzhagen
Analysing computations in neural circuits often uses simplified models because the actual neuronal implementation is not known. For example, a problem in vision, how the eye detects image motion, has long been analysed using Hassenstein-Reichardt (HR) detector or Barlow-Levick (BL) models. These both simulate motion detection well, but the exact neuronal circuits undertaking these tasks remain elusive. We reconstructed a comprehensive connectome of the circuits of Drosophila's motion-sensing T4 cells using a novel EM technique...
April 22, 2017: ELife
https://www.readbyqxmd.com/read/28432730/assessment-of-voluntary-deep-inspiration-breath-hold-with-cine-imaging-for-breast-radiotherapy
#18
Reuben Patrick Estoesta, Lani Attwood, Diana Naehrig, Elizabeth Claridge-Mackonis, David Odgers, Darren Martin, Melissa Pham, Joanne Toohey, Susan Carroll
Deep Inspiration Breath-Hold (DIBH) techniques for breast cancer radiation therapy (RT) have reduced cardiac dose compared to Free Breathing (FB). Recently, a voluntary deep inspiration breath-hold (vDIBH) technique was established using in-room lasers and skin tattoos to monitor breath-hold. An in-house quality assessment of positional reproducibility during RT delivery with vDIBH in patients with left-sided breast cancer was evaluated. The electronic portal imaging device (EPID) was used in cinematographic (CINE) mode to capture a sequence of images during beam delivery...
April 22, 2017: Journal of Medical Imaging and Radiation Oncology
https://www.readbyqxmd.com/read/28432654/laser-desorption-ionization-mass-spectrometric-imaging-of-endogenous-lipids-from-rat-brain-tissue-implanted-with-silver-nanoparticles
#19
Ludovic Muller, Kathrine Baldwin, Damon C Barbacci, Shelley N Jackson, Aurélie Roux, Carey D Balaban, Bruce E Brinson, Michael I McCully, Ernest K Lewis, J Albert Schultz, Amina S Woods
Mass spectrometry imaging (MSI) of tissue implanted with silver nanoparticulate (AgNP) matrix generates reproducible imaging of lipids in rodent models of disease and injury. Gas-phase production and acceleration of size-selected 8 nm AgNP is followed by controlled ion beam rastering and soft landing implantation of 500 eV AgNP into tissue. Focused 337 nm laser desorption produces high quality images for most lipid classes in rat brain tissue (in positive mode: galactoceramides, diacylglycerols, ceramides, phosphatidylcholines, cholesteryl ester, and cholesterol, and in negative ion mode: phosphatidylethanolamides, sulfatides, phosphatidylinositol, and sphingomyelins)...
April 21, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28432568/the-musos-music-software-system-toolkit-a-computer-based-open-source-application-for-testing-memory-for-melodies
#20
M Rainsford, M A Palmer, G Paine
Despite numerous innovative studies, rates of replication in the field of music psychology are extremely low (Frieler et al., 2013). Two key methodological challenges affecting researchers wishing to administer and reproduce studies in music cognition are the difficulty of measuring musical responses, particularly when conducting free-recall studies, and access to a reliable set of novel stimuli unrestricted by copyright or licensing issues. In this article, we propose a solution for these challenges in computer-based administration...
April 21, 2017: Behavior Research Methods
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