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urine proteomics

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https://www.readbyqxmd.com/read/28821634/lipidomic-and-proteomic-analysis-of-exosomes-from-mouse-cortical-collecting-duct-cells
#1
Viet D Dang, Kishore Kumar Jella, Ragy R T Ragheb, Nancy D Denslow, Abdel A Alli
Exosomes are endosome-derived nanovesicles that are involved in cellular communication and signaling. Exosomes are produced by epithelial cells and are found in biologic fluids including blood and urine. The packaged material within exosomes includes proteins and lipids, but the molecular comparison within exosome subtypes is largely unknown. The purpose of this study was to investigate differences between exosomes derived from the apical plasma membrane and basolateral plasma membrane of polarized murine cortical collecting duct principal cells...
August 16, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28815030/a-combined-bile-and-urine-proteomic-test-for-cholangiocarcinoma-diagnosis-in-patients-with-biliary-strictures-of-unknown-origin
#2
Torsten Voigtländer, Jochen Metzger, Bastian Schönemeier, Mark Jäger, Harald Mischak, Michael P Manns, Tim O Lankisch
BACKGROUND: Detection of cholangiocarcinoma (CC) remains a diagnostic challenge particularly in patients with primary sclerosing cholangitis (PSC). We recently established diagnostic peptide marker models in bile and urine to detect CC. Our aim was to combine both models to reach a higher diagnostic accuracy of CC diagnosis. METHODS: Bile (BPA) and urine (UPA) proteome analysis by capillary electrophoresis mass spectrometry was performed in a case-control phase II study on 87 patients (36 CC including 13 with CC on top of PSC, 33 PSC and 18 other benign disorders)...
August 2017: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/28798909/analysis-of-urine-composition-in-type-ii-diabetic-mice-after-intervention-therapy-using-holothurian-polypeptides
#3
Yanyan Li, Jiajie Xu, Xiurong Su
Hydrolysates and peptide fractions (PF) obtained from sea cucumber with commercial enzyme were studied on the hyperglycemic and renal protective effects on db/db rats using urine metabolomics. Compared with the control group the polypeptides from the two species could significantly reduce the urine glucose and urea. We also tried to address the compositions of highly expressed urinary proteins using a proteomics approach. They were serum albumins, AMBP proteins, negative trypsin, elastase, and urinary protein, GAPDH, a receptor of urokinase-type plasminogen activator (uPAR), and Ig kappa chain C region...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/28794178/potential-of-high-affinity-slow-off-rate-modified-aptamer-somamer-reagents-for-mycobacterium-tuberculosis-proteins-as-tools-for-infection-models-and-diagnostic-applications
#4
Theresa Russell, Louis S Green, Taylor Rice, Nicole A Kruh-Garcia, Karen Dobos, Mary A DeGroote, Thomas Hraha, David G Sterling, Nebojsa Janjic, Urs A Ochsner
Direct pathogen detection in blood to diagnose active tuberculosis (TB) has been difficult, due to low levels of circulating antigens or due to the lack of specific, high-affinity binding reagents and reliable assays with adequate sensitivity. We sought to determine whether slow off-rate modified aptamer (SOMAmer) reagents with sub-nanomolar affinity for M. tuberculosis (Mtb) proteins (antigen 85A, 85B, 85C, GroES, GroEL2, DnaK, CFP10, KAD, CFP2, RplL and Tpx) could be useful to diagnose tuberculosis. When incorporated into the multiplexed, array-based proteomic SOMAscan assay, limits of detection reached the sub-picomolar range in 40% serum...
August 9, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28780902/emerging-ms-based-platforms-for-the-characterization-of-tumor-derived-exosomes-isolated-from-human-biofluids-challenges-and-promises-of-mudpit
#5
Emanuele Ferrari, Antonella De Palma, Pierluigi Mauri
Exosomes are small extracellular vesicles of endosomal origin that are produced and released by several type of cells. These vesicles contain different macromolecules: proteins, mRNA, miRNA, mitochondrial DNA, and lipids. Exosomes play an important role in cell-to-cell communication, also promoting cancer progression. Areas covered: Various proteomic approaches have been applied to study exosomes isolated from different human biofluids in search of possible cancer biomarkers. The results of these studies are reported, and pros and cons of each employed technique are described...
August 7, 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/28775000/bpi-fold-containing-family-a-member-2-parotid-secretory-protein-is-an-early-biomarker-of-aki
#6
Satya K Kota, Elizabeth Pernicone, David E Leaf, Isaac E Stillman, Sushrut S Waikar, Savithri Balasubramanian Kota
AKI is a major cause of morbidity and mortality and an important contributor to the development and progression of CKD. Molecular biomarkers that improve the detection and prognostication of AKI are therefore required. We assessed the utility as such of BPI fold-containing family A member 2 (BPIFA2), also known as parotid secretory protein, which we identified via a multiplex quantitative proteomics screen of acutely injured murine kidneys. In physiologic conditions, BPIFA2 is expressed specifically in the parotid glands and is abundant in salivary secretions...
August 3, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/28752056/the-munich-midy-pig-biobank-a-unique-resource-for-studying-organ-crosstalk-in-diabetes
#7
Andreas Blutke, Simone Renner, Florian Flenkenthaler, Mattias Backman, Serena Haesner, Elisabeth Kemter, Erik Ländström, Christina Braun-Reichhart, Barbara Albl, Elisabeth Streckel, Birgit Rathkolb, Cornelia Prehn, Alessandra Palladini, Michal Grzybek, Stefan Krebs, Stefan Bauersachs, Andrea Bähr, Andreas Brühschwein, Cornelia A Deeg, Erica De Monte, Michaela Dmochewitz, Caroline Eberle, Daniela Emrich, Robert Fux, Frauke Groth, Sophie Gumbert, Antonia Heitmann, Arne Hinrichs, Barbara Keßler, Mayuko Kurome, Miriam Leipig-Rudolph, Kaspar Matiasek, Hazal Öztürk, Christiane Otzdorff, Myriam Reichenbach, Horst Dieter Reichenbach, Alexandra Rieger, Birte Rieseberg, Marco Rosati, Manuel Nicolas Saucedo, Anna Schleicher, Marlon R Schneider, Kilian Simmet, Judith Steinmetz, Nicole Übel, Patrizia Zehetmaier, Andreas Jung, Jerzy Adamski, Ünal Coskun, Martin Hrabě de Angelis, Christian Simmet, Mathias Ritzmann, Andrea Meyer-Lindenberg, Helmut Blum, Georg J Arnold, Thomas Fröhlich, Rüdiger Wanke, Eckhard Wolf
OBJECTIVE: The prevalence of diabetes mellitus and associated complications is steadily increasing. As a resource for studying systemic consequences of chronic insulin insufficiency and hyperglycemia, we established a comprehensive biobank of long-term diabetic INS(C94Y) transgenic pigs, a model of mutant INS gene-induced diabetes of youth (MIDY), and of wild-type (WT) littermates. METHODS: Female MIDY pigs (n = 4) were maintained with suboptimal insulin treatment for 2 years, together with female WT littermates (n = 5)...
August 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/28729428/fluorine-18-labeled-carboplatin-derivative-for-pet-imaging-of-platinum-drug-distribution
#8
Narottam Lamichhane, Gajanan K Dewkar, Sundaresan Gobalakrishnan, Li Wang, Purnima Jose, Muhammad Otabashi, Jean-Luc Morelle, Nicholas Farrell, Jamal Zweit
Increasing evidence indicates that reduced intracellular drug accumulation is the parameter most consistently associated with platinum drug resistance, and emphasizes the need to directly measure intra-tumor drug concentration. In the era of precision medicine and with the advent of powerful imaging and proteomics technologies, there is an opportunity to better understand drug resistance, by exploiting these techniques to provide new knowledge on drug-target interactions. Here, we are contributing to this endeavor by reporting on the development of a fluorine-18 labeled carboplatin derivative ((18)F-FCP) that can be used to potentially image drug uptake and retention, including intra-tumoral distribution, by positron emission tomography (PET)...
July 20, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28727760/detection-of-titin-fragments-in-urine-in-response-to-exercise-induced-muscle-damage
#9
Kazue Kanda, Jun Sakuma, Takayuki Akimoto, Yasuo Kawakami, Katsuhiko Suzuki
Many studies have attempted to determine the associations between blood biomarkers and exercise-induced muscle damage. However, poor correlations between the changes in biomarker levels and the magnitude of muscle symptoms have been reported. Recent advances in proteomic tools offer a strategy for the comprehensive analysis of protein expression, which can be used to identify biomarkers. Here, we used a proteomic analysis to identify urinary proteins that appear in response to a calf-raise exercise, including repetitive eccentric muscle contractions, and found that a titin (also known as connectin) N-terminal fragment molecule appears in the urine after eccentric exercise...
2017: PloS One
https://www.readbyqxmd.com/read/28717416/feasibility-of-urinary-extracellular-vesicle-proteome-profiling-using-a-robust-and-simple-clinically-applicable-isolation-method
#10
Irene V Bijnsdorp, Olga Maxouri, Aarzo Kardar, Tim Schelfhorst, Sander R Piersma, Thang V Pham, Andre Vis, R Jeroen van Moorselaar, Connie R Jimenez
Extracellular vesicles (EVs) secreted by prostate cancer (PCa) cells contain specific biomarkers and can be isolated from urine. Collection of urine is not invasive, and therefore urinary EVs represent a liquid biopsy for diagnostic and prognostic testing for PCa. In this study, we optimised urinary EV isolation using a method based on heat shock proteins and compared it to gold-standard ultracentrifugation. The urinary EV isolation protocol using the Vn96-peptide is easier, time convenient (≈1.5 h) and no special equipment is needed, in contrast to ultracentrifugation protocol (>3...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28711247/ratio-of-dietary-rumen-degradable-protein-to-rumen-undegradable-protein-affects-nitrogen-partitioning-but-does-not-affect-the-bovine-milk-proteome-produced-by-mid-lactation-holstein-dairy-cows
#11
R Tacoma, J Fields, D B Ebenstein, Y-W Lam, S L Greenwood
Little is known about the bovine milk proteome or whether it can be affected by diet. The objective of this study was to determine if the dietary rumen degradable protein (RDP):rumen undegradable protein (RUP) ratio could alter the bovine milk proteome. Six Holstein cows (parity: 2.5 ± 0.8) in mid lactation were blocked by days in milk (80 ± 43 d in milk) and milk yield (57.5 ± 6.0 kg) and randomly assigned to treatment groups. The experiment was conducted as a double-crossover design consisting of three 21-d periods...
September 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28681595/assessment-of-the-therapeutic-potential-of-persimmon-leaf-extract-on-prediabetic-subjects
#12
Mohd M Khan, Bao Quoc Tran, Yoon-Jin Jang, Soo-Hyun Park, William E Fondrie, Khadiza Chowdhury, Sung Hwan Yoon, David R Goodlett, Soo-Wan Chae, Han-Jung Chae, Seung-Young Seo, Young Ah Goo
Dietary supplements have exhibited myriads of positive health effects on human health conditions and with the advent of new technological advances, including in the fields of proteomics, genomics, and metabolomics, biological and pharmacological activities of dietary supplements are being evaluated for their ameliorative effects in human ailments. Recent interests in understanding and discovering the molecular targets of phytochemical-gene-protein-metabolite dynamics resulted in discovery of a few protein signature candidates that could potentially be used to assess the effects of dietary supplements on human health...
July 31, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28674889/proteomics-analysis-of-circulating-serum-exosomes
#13
Antonius Koller, Purvi Patel, Jenny Kim Kim, Emily I Chen
Proteomics characterization of biofluids, such as urine and plasma, has been explored for the discovery of predictive, prognostic, and mechanistic biomarkers of diseases and tissue injury. Here we describe comprehensive characterization of protein cargos from cell-derived secreted vesicles (extracellular vesicles or exosome) for biomarker discovery using the mass spectrometry-based technology.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28669814/quick-96fasp-for-high-throughput-quantitative-proteome-analysis
#14
Yanbao Yu, Shiferaw Bekele, Rembert Pieper
Filter aided sample preparation (FASP) is becoming a central method for proteomic sample cleanup and peptide generation prior to LC-MS analysis. We previously adapted this method to a 96-well filter plate, and applied to prepare protein digests from cell lysate and body fluid samples in a high throughput quantitative manner. While the 96FASP approach is scalable and can handle multiple samples simultaneously, two key advantages compared to single FASP, it is also time-consuming. The centrifugation-based liquid transfer on the filter plate takes 3-5 times longer than single filter...
June 29, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28665768/identification-of-a-sensitive-urinary-biomarker-selenium-binding-protein-1-for-early-detection-of-acute-kidney-injury
#15
Kyeong Seok Kim, Hun Yong Yang, Hosup Song, Ye Rim Kang, JiHoon Kwon, JiHye An, Ji Yeon Son, Seung Jun Kwack, Young-Mi Kim, Ok-Nam Bae, Mee-Young Ahn, Jaewon Lee, Sungpil Yoon, Byung Mu Lee, Hyung Sik Kim
Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i...
June 30, 2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/28635333/molecular-biomarkers-to-guide-precision-medicine-in-localized-prostate-cancer
#16
Minke Smits, Niven Mehra, Michiel Sedelaar, Winald Gerritsen, Jack A Schalken
Major advances through tumor profiling technologies, that include next-generation sequencing, epigenetic, proteomic and transcriptomic methods, have been made in primary prostate cancer, providing novel biomarkers that may guide precision medicine in the near future. Areas covered: The authors provided an overview of novel molecular biomarkers in tissue, blood and urine that may be used as clinical tools to assess prognosis, improve selection criteria for active surveillance programs, and detect disease relapse early in localized prostate cancer...
August 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28631898/mass-spectrometry-based-protein-analytics-to-unravel-the-tissue-pathophysiology-in-duchenne-muscular-dystrophy
#17
Stephanie J Carr, René P Zahedi, Hanns Lochmüller, Andreas Roos
Duchenne muscular dystrophy (DMD) is a genetic muscle wasting condition with limited treatment options available and is caused by the lack of Dystrophin. However, pathophysiology of different tissues is variable showing different histological and molecular signatures. Recently, a number of studies have employed gel-free proteomic approaches to unveil the molecular pathophysiology in terms of tissue-specific proteome changes in dystrophin-deficiency. We analysed studies in models of dystrophin-deficiency and patients both from the published literature...
June 20, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28618237/effect-of-sweetened-dried-cranberry-consumption-on-urinary-proteome-and-fecal-microbiome-in-healthy-human-subjects
#18
Nell Bekiares, Christian G Krueger, Jennifer J Meudt, Dhanansayan Shanmuganayagam, Jess D Reed
The relationship among diet, human health, and disease is an area of growing interest in biomarker research. Previous studies suggest that the consumption of cranberries (Vaccinium macrocarpon) could beneficially influence urinary and digestive health. The present study sought to determine if daily consumption of sweetened dried cranberries (SDC) changes the urinary proteome and fecal microbiome, as determined in a prospective sample of 10 healthy individuals. Baseline urine and fecal samples were collected from the subjects in the fasted (8-12 h) state...
June 15, 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/28596590/a-comprehensive-analysis-and-annotation-of-human-normal-urinary-proteome
#19
Mindi Zhao, Menglin Li, Yehong Yang, Zhengguang Guo, Ying Sun, Chen Shao, Mingxi Li, Wei Sun, Youhe Gao
Biomarkers are measurable changes associated with the disease. Urine can reflect the changes of the body while blood is under control of the homeostatic mechanisms; thus, urine is considered an important source for early and sensitive disease biomarker discovery. A comprehensive profile of the urinary proteome will provide a basic understanding of urinary proteins. In this paper, we present an in-depth analysis of the urinary proteome based on different separation strategies, including direct one dimensional liquid chromatography-tandem mass spectrometry (LC/MS/MS), two dimensional LC/MS/MS, and gel-eluted liquid fraction entrapment electrophoresis/liquid-phase isoelectric focusing followed by two dimensional LC/MS/MS...
June 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28595723/stable-isotope-n-phosphoryl-amino-acids-labeling-for-quantitative-profiling-of-amine-containing-metabolites-using-liquid-chromatography-mass-spectrometry
#20
Shanshan Zhang, Jinwen Shi, Changkai Shan, Chengting Huang, Yile Wu, Rong Ding, Yuhua Xue, Wen Liu, Qiang Zhou, Yufen Zhao, Pengxiang Xu, Xiang Gao
Stable isotope chemical labeling liquid chromatography-mass spectrometry (LC-MS) is a powerful strategy for comprehensive metabolomics profiling, which can improve metabolites coverage and quantitative information for exploration of metabolic regulation in complex biological systems. In the current work, a novel stable isotope N-phosphoryl amino acids labeling strategy (SIPAL) has been successful developed for quantitative profiling of amine-containing metabolites in urine based on organic phosphorus chemistry...
July 25, 2017: Analytica Chimica Acta
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