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urine proteomics

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https://www.readbyqxmd.com/read/29028400/label-free-study-of-cosmonaut-s-urinary-proteome-changes-after-long-duration-spaceflights
#1
A Brzhozovskiy, A Kononikhin, M Indeykina, LKh Pastushkova, I A Popov, E N Nikolaev, I M Larina
During the entire time that cosmonauts stay on board the international space station, different extreme space flight factors affect their bodies. In order to find out what physiological changes occur under the influence of spaceflight, different parameters of the human body before and after flights are monitored. Analysis of the urine proteome is one of the most perspective non-invasive methods of condition monitoring. The aim of the study was to perform a comparative semi-quantitative label-free urine proteome analysis of samples collected from 21 cosmonauts before and after long-duration spaceflight at the international space station...
August 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29028163/early-detection-of-urinary-proteome-biomarkers-for-effective-early-treatment-of-pulmonary-fibrosis-in-a-rat-model
#2
Jianqiang Wu, Xundou Li, Mindi Zhao, He Huang, Wei Sun, Youhe Gao
PURPOSE: Pulmonary fibrosis (PF) is a progressive and devastating lung disease. With limited effective treatments available in the late stage, PF has a very poor prognosis. Molecular biomarkers are highly desired for PF, especially for its early phase. MATERIAL AND METHODS: Urine is a good biomarker source, and accumulates systemic changes in the body especially in the early-stage of diseases. In this study, a bleomycin (BLM)-induced rat model was used to mimic PF...
October 13, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28994030/gelc-ms-a-sample-preparation-method-for-proteomics-analysis-of-minimal-amount-of-tissue
#3
Manousos Makridakis, Antonia Vlahou
Application of various proteomics methodologies have been implemented for the global and targeted proteome analysis of many different types of biological samples such as tissue, urine, plasma, serum, blood, and cell lines. Among the aforementioned biological samples, tissue has an exceptional role into clinical research and practice. Disease initiation and progression is usually located at the tissue level of different organs, making the analysis of this material very important for the understanding of the disease pathophysiology...
October 10, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28992005/characterization-of-exosomes-from-body-fluids-of-dairy-cows
#4
Y Q Koh, H N Peiris, K Vaswani, S Meier, C R Burke, K A Macdonald, J R Roche, F Almughlliq, B J Arachchige, S Reed, M D Mitchell
Exosomes are a specific subpopulation of extracellular vesicles that are widely released by cells of different origins with divergent functions that make their way into body fluids that can be conveniently sampled. In the current study, we isolated and evaluated exosomes from concurrently collected samples of milk, plasma, saliva, and urine from a group of 6 pregnant Holstein-Friesian dairy cows (aged 7 mo, 174 to 203 d of gestation). The cows had BCS of 3.5 to 5.25 (on a scale of 1 to 10), and the milk production for the season to the time of sampling ranged between 5,118 and 6,959 kg...
September 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28980450/dynamic-changes-of-urine-proteome-in-a-walker-256-tumor-bearing-rat-model
#5
Jianqiang Wu, Zhengguang Guo, Youhe Gao
Despite advances in cancer treatments, early diagnosis of cancer is still the most promising way to improve outcomes. Without homeostatic control, urine reflects systemic changes in the body and can potentially be used for early detection of cancer. In this study, a tumor-bearing rat model was established by subcutaneous injection of Walker 256 cells. Urine samples from tumor-bearing rats were collected at five time points during cancer development. Dynamic urine proteomes were profiled using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)...
October 4, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28979691/fascin-1-is-released-from-proximal-tubular-cells-in-response-to-calcineurin-inhibitors-cnis-and-correlates-with-isometric-vacuolization-in-kidney-transplanted-patients
#6
Conxita Jacobs-Cachá, Irina B Torres, Joan López-Hellín, Carme Cantarell, María A Azancot, Antonio Román, Francesc Moreso, Daniel Serón, Anna Meseguer, Eduard Sarró
Immunosuppression based on calcineurin inhibitors (CNIs) has greatly improved organ transplantation, although subsequent nephrotoxicity significantly hinders treatment success. There are no currently available specific soluble biomarkers for CNI-induced nephrotoxicity and diagnosis relies on renal biopsy, which is costly, invasive and may cause complications. Accordingly, identification of non-invasive biomarkers distinguishing CNI-induced kidney tubular damage from that of other etiologies would greatly improve diagnosis and enable more precise dosage adjustment...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28974115/application-of-proteomics-to-graft-versus-host-disease-from-biomarker-discovery-to-potential-clinical-applications
#7
Richard B Presland
Graft-versus-host disease (GVHD) is a frequent and potentially life-threatening complication that occurs in many patients who undergo hematopoietic stem cell transplantation. In an effort to develop blood and tissue-based biochemical assays for GVHD diagnosis, high throughput proteomic platforms have been widely utilized for the identification and validation of disease biomarkers for both acute and chronic GVHD. Areas covered: This article reviews biomarker research findings on acute and chronic GVHD ascertained by studying peripheral blood, urine and saliva that gives biological information on systemic or localized disease...
October 4, 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/28927277/benchmark-dose-modeling-estimates-of-the-concentrations-of-inorganic-arsenic-that-induce-changes-to-the-neonatal-transcriptome-proteome-and-epigenome-in-a-pregnancy-cohort
#8
Julia E Rager, Scott S Auerbach, Grace A Chappell, Elizabeth Martin, Chad M Thompson, Rebecca C Fry
Prenatal inorganic arsenic (iAs) exposure influences the expression of critical genes and proteins associated with adverse outcomes in newborns, in part through epigenetic mediators. The doses at which these genomic and epigenomic changes occur have yet to be evaluated in the context of dose-response modeling. The goal of the present study was to estimate iAs doses that correspond to changes in transcriptomic, proteomic, epigenomic, and integrated multi-omic signatures in human cord blood through benchmark dose (BMD) modeling...
September 27, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28917266/urinary-biomarkers-in-prostate-cancer-detection-and-monitoring-progression
#9
REVIEW
Duojia Wu, Jie Ni, Julia Beretov, Paul Cozzi, Mark Willcox, Valerie Wasinger, Bradley Walsh, Peter Graham, Yong Li
Prostate cancer (CaP) is the most common cancer in men and the second leading cause of cancer deaths in males in Australia. Although serum prostate-specific antigen (PSA) has been the most widely used biomarker in CaP detection for decades, PSA screening has limitations such as low specificity and potential association with over-diagnosis. Current biomarkers used in the clinic are not useful for the early detection of CaP, or monitoring its progression, and have limited value in predicting response to treatment...
October 2017: Critical Reviews in Oncology/hematology
https://www.readbyqxmd.com/read/28912895/mass-spectrometry-assisted-gel-based-proteomics-in-cancer-biomarker-discovery-approaches-and-application
#10
REVIEW
Rongrong Huang, Zhongsi Chen, Lei He, Nongyue He, Zhijiang Xi, Zhiyang Li, Yan Deng, Xin Zeng
There is a critical need for the discovery of novel biomarkers for early detection and targeted therapy of cancer, a major cause of deaths worldwide. In this respect, proteomic technologies, such as mass spectrometry (MS), enable the identification of pathologically significant proteins in various types of samples. MS is capable of high-throughput profiling of complex biological samples including blood, tissues, urine, milk, and cells. MS-assisted proteomics has contributed to the development of cancer biomarkers that may form the foundation for new clinical tests...
2017: Theranostics
https://www.readbyqxmd.com/read/28899687/proteomic-profiling-of-sweat-exosome-suggests-its-involvement-in-skin-immunity
#11
Chang-Xian Wu, Zheng-Fei Liu
The healthy human skin sustains an effective immune defense mechanism, formed by a complex physical and chemical epidermal barrier that coordinates with different cellular components of the skin immune system. However, the mechanism how skin cells regulate local immune homeostasis in health and disease contexts is not well known. To investigate whether exosomes exist in sweat, sweat samples from healthy individuals were collected after aerobic exercise. Sweat exosome was isolated via differential ultracentrifugation, observed under transmission electron microscope, measured by dynamic light scattering and confirmed by immunoblot...
September 9, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28885000/a-multiplatform-approach-for-the-discovery-of-novel-drug-induced-kidney-injury-biomarkers
#12
Liuxi Chen, James Smith, Jaromir Mikl, Ryan Fryer, Frank Pack, Brad J Williams, Jonathan A Phillips, Vladimir V Papov
Drug-induced kidney injury (DIKI) is a common toxicity observed in pharmaceutical development. We demonstrated the use of label-free liquid chromatography-mass spectrometry (LC-MS) and multiplex liquid chromatography-single reaction monitoring (LC-SRM) as practical extensions of standard immunoassay based safety biomarker assessments for identification of new toxicity marker candidates and for improved mechanistic understanding. Two different anticancer drugs, doxorubicin (DOX) and cisplatin (cis-diamminedichloridoplatinum, CDDP), were chosen as the toxicants due to their different modes of nephrotoxicity...
September 27, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28880921/urinary-proteomics-in-predicting-heart-transplantation-outcomes-uprophet-rationale-and-database-description
#13
Qi-Fang Huang, Sander Trenson, Zhen-Yu Zhang, Wen-Yi Yang, Lucas Van Aelst, Esther Nkuipou-Kenfack, Fang-Fei Wei, Blerim Mujaj, Lutgarde Thijs, Agnieszka Ciarka, Jerome Zoidakis, Walter Droogné, Antonia Vlahou, Stefan Janssens, Johan Vanhaecke, Johan Van Cleemput, Jan A Staessen
OBJECTIVES: Urinary Proteomics in Predicting Heart Transplantation Outcomes (uPROPHET; NCT03152422) aims: (i) to construct new multidimensional urinary proteomic (UP) classifiers that after heart transplantation (HTx) help in detecting graft vasculopathy, monitoring immune system activity and graft performance, and in adjusting immunosuppression; (ii) to sequence UP peptide fragments and to identify key proteins mediating HTx-related complications; (iii) to validate UP classifiers by demonstrating analogy between UP profiles and tissue proteomic signatures (TP) in diseased explanted hearts, to be compared with normal donor hearts; (iv) and to identify new drug targets...
2017: PloS One
https://www.readbyqxmd.com/read/28869421/a-systemic-view-on-the-distribution-of-diet-derived-methanol-and-hepatic-acetone-in-mice
#14
Martin Kistler, Andreea Muntean, Vera Hoellriegl, Georg Matuschek, Ralf Zimmermann, Christoph Hoeschen, Martin Hrabe de Angelis, Jan Rozman
Volatile organic compounds (VOCs) from breath can successfully be used to diagnose disease-specific pathological alterations in metabolism. However, the exact origin and underlying biochemical pathways that could be mapped to VOC signatures are mainly unknown. There is a knowledge gap regarding the contribution of tissues, organs, the gut microbiome, and exogenous factors to the "sum signal" from breath samples. Animal models for human disease such as mutant mice provide the possibility to reproduce genetic predisposition to disease, thereby allowing the in-depth analysis of metabolic and biochemical functions...
September 4, 2017: Journal of Breath Research
https://www.readbyqxmd.com/read/28827195/impact-of-regular-cannabis-use-on-biomarkers-of-lower-urinary-tract-function
#15
Balachandar Nedumaran, Pratyaydipta Rudra, Jeanette Gaydos, Sushil Kumar, Randall B Meacham, Ellen L Burnham, Anna P Malykhina
OBJECTIVE: To evaluate the differences in the composition and quantities of urine peptides in regular cannabis users and nonusers by liquid chromatography tandem mass spectrometry analysis. MATERIALS AND METHODS: Urine specimens from healthy control subjects and cannabis users were utilized to identify the differences in the number and quantity of urine proteins by liquid chromatography tandem mass spectrometry analysis. Significantly altered proteins were determined by a permutation testing statistical method...
August 18, 2017: Urology
https://www.readbyqxmd.com/read/28821634/lipidomic-and-proteomic-analysis-of-exosomes-from-mouse-cortical-collecting-duct-cells
#16
Viet D Dang, Kishore Kumar Jella, Ragy R T Ragheb, Nancy D Denslow, Abdel A Alli
Exosomes are endosome-derived nanovesicles that are involved in cellular communication and signaling. Exosomes are produced by epithelial cells and are found in biologic fluids including blood and urine. The packaged material within exosomes includes proteins and lipids, but the molecular comparison within exosome subtypes is largely unknown. The purpose of this study was to investigate differences between exosomes derived from the apical plasma membrane and basolateral plasma membrane of polarized murine cortical collecting duct principal cells...
August 16, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28815030/a-combined-bile-and-urine-proteomic-test-for-cholangiocarcinoma-diagnosis-in-patients-with-biliary-strictures-of-unknown-origin
#17
Torsten Voigtländer, Jochen Metzger, Bastian Schönemeier, Mark Jäger, Harald Mischak, Michael P Manns, Tim O Lankisch
BACKGROUND: Detection of cholangiocarcinoma (CC) remains a diagnostic challenge particularly in patients with primary sclerosing cholangitis (PSC). We recently established diagnostic peptide marker models in bile and urine to detect CC. Our aim was to combine both models to reach a higher diagnostic accuracy of CC diagnosis. METHODS: Bile (BPA) and urine (UPA) proteome analysis by capillary electrophoresis mass spectrometry was performed in a case-control phase II study on 87 patients (36 CC including 13 with CC on top of PSC, 33 PSC and 18 other benign disorders)...
August 2017: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/28798909/analysis-of-urine-composition-in-type-ii-diabetic-mice-after-intervention-therapy-using-holothurian-polypeptides
#18
Yanyan Li, Jiajie Xu, Xiurong Su
Hydrolysates and peptide fractions (PF) obtained from sea cucumber with commercial enzyme were studied on the hyperglycemic and renal protective effects on db/db rats using urine metabolomics. Compared with the control group the polypeptides from the two species could significantly reduce the urine glucose and urea. We also tried to address the compositions of highly expressed urinary proteins using a proteomics approach. They were serum albumins, AMBP proteins, negative trypsin, elastase, and urinary protein, GAPDH, a receptor of urokinase-type plasminogen activator (uPAR), and Ig kappa chain C region...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/28794178/potential-of-high-affinity-slow-off-rate-modified-aptamer-reagents-for-mycobacterium-tuberculosis-proteins-as-tools-for-infection-models-and-diagnostic-applications
#19
Theresa M Russell, Louis S Green, Taylor Rice, Nicole A Kruh-Garcia, Karen Dobos, Mary A De Groote, Thomas Hraha, David G Sterling, Nebojsa Janjic, Urs A Ochsner
Direct pathogen detection in blood to diagnose active tuberculosis (TB) has been difficult due to low levels of circulating antigens or due to the lack of specific, high-affinity binding reagents and reliable assays with adequate sensitivity. We sought to determine whether slow off-rate modified aptamer (SOMAmer) reagents with subnanomolar affinity for Mycobacterium tuberculosis proteins (antigens 85A, 85B, 85C, GroES, GroEL2, DnaK, CFP10, KAD, CFP2, RplL, and Tpx) could be useful to diagnose tuberculosis. When incorporated into the multiplexed, array-based proteomic SOMAscan assay, limits of detection reached the subpicomolar range in 40% serum...
October 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28780902/emerging-ms-based-platforms-for-the-characterization-of-tumor-derived-exosomes-isolated-from-human-biofluids-challenges-and-promises-of-mudpit
#20
Emanuele Ferrari, Antonella De Palma, Pierluigi Mauri
Exosomes are small extracellular vesicles of endosomal origin that are produced and released by several type of cells. These vesicles contain different macromolecules: proteins, mRNA, miRNA, mitochondrial DNA, and lipids. Exosomes play an important role in cell-to-cell communication, also promoting cancer progression. Areas covered: Various proteomic approaches have been applied to study exosomes isolated from different human biofluids in search of possible cancer biomarkers. The results of these studies are reported, and pros and cons of each employed technique are described...
August 10, 2017: Expert Review of Proteomics
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