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Anne Z Phillips, Jeffrey C Berry, Mark C Wilson, Anupama Vijayaraghavan, Jillian Burke, J Imani Bunn, Tom W Allen, Terry Wheeler, Rebecca S Bart
Cotton bacterial blight (CBB), an important disease of (Gossypium hirsutum) in the early 20th century, had been controlled by resistant germplasm for over half a century. Recently, CBB re-emerged as an agronomic problem in the United States. Here, we report analysis of cotton variety planting statistics that indicate a steady increase in the percentage of susceptible cotton varieties grown each year since 2009. Phylogenetic analysis revealed that strains from the current outbreak cluster with race 18 Xanthomonas citri pv...
September 14, 2017: PLoS Genetics
Graciete S Silva, Denilson J Assis, Janice I Druzian, Maria B P P Oliveira, Paulo L L Ribeiro, Soraia M Cordeiro, Cleber A Schmidt
The conditions of storage, cultivation and maintenance of microbial cultures should preserve the microbiological homogeneity, phenotypic and genotypic characteristics to ensure better reproducibility of metabolic production. To evaluate the influence of the storage condition on the composition of cell fatty acids, genetic profile and biochemical characteristics of Xanthomonas campestris pv. mangiferaeindicae IBSBF 2103, as well as, to identify its relationship with the yielding and viscosity of the xanthan gum produced, this study monitored the strain preserved in two simple and widely used conditions, ultra-freezer (-80 °C) and refrigeration (3-8 °C) during 5 months...
September 2017: Indian Journal of Microbiology
Jie Kuang, Jianzhong Liu, Jun Mei, Changchun Wang, Haitao Hu, Yanjun Zhang, Meihao Sun, Xi Ning, Langtao Xiao, Ling Yang
Bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most devastating diseases of rice. However, the molecular mechanism underpinning the Xoo resistance of rice is still not fully understood. Here, we report that a class II small heat shock protein gene, OsHsp18.0, whose expression was differentially induced between a resistant and a susceptible variety in response to Xoo infection, plays positive roles in both biotic and abiotic resistance. The molecular chaperone activity of OsHsp18...
September 12, 2017: Scientific Reports
Alex Schultink, Tiancong Qi, Arielle Lee, Adam D Steinbrenner, Brian Staskawicz
Xanthomonas spp are phytopathogenic bacteria that can cause disease on a wide variety of plant species resulting in significant impacts on crop yields. Limited genetic resistance is available in most crop species and current control methods are often inadequate, particularly when environmental conditions favor disease. The plant Nicotiana benthamiana has been shown to be resistant to Xanthomonas and Pseudomonas due to an immune response triggered by the bacterial effector proteins XopQ and HopQ1, respectively...
September 11, 2017: Plant Journal: for Cell and Molecular Biology
Shi-Qi An, Ji-Liang Tang, J Maxwell Dow
The determination of the genome sequences of pathogenic bacteria has facilitated functional analyses that aim to understand the molecular basis of virulence. In particular, genome sequence information of the pathogen Xanthomonas campestris pathovar campestris has allowed researchers to identify and functionally analyze the role of intracellular signaling involving cyclic di-GMP in black rot disease of crucifers. Here, we describe leaf clipping and spraying methods for testing the virulence of wild type and derived mutants of X...
2017: Methods in Molecular Biology
Mylène Ruh, Martial Briand, Sophie Bonneau, Marie-Agnès Jacques, Nicolas W G Chen
Xanthomonas citri pv. vignicola strains cause bacterial blight of the legume crop cowpea. We report whole-genome sequences of three X. citri pv. vignicola strains obtained using PacBio single-molecule real-time sequencing. Such genomic data provide new information on pathogenicity factors, such as transcription activator-like effectors.
September 7, 2017: Genome Announcements
Huan Du, Zhijiang Fan, Lan Yang, Xiaoping Bao
A series of novel quinazolin-4-one derivatives (7a-7n) bearing the 7-oxo-1,2,4-triazolo[1,5-a]pyrimidine moiety were designed, synthesized and evaluated for their inhibition activities against phytopathogenic bacteria and fungi in vitro. All of the target compounds were fully characterized through [Formula: see text] NMR, [Formula: see text] NMR, HRMS and IR spectra. Among these compounds, the structure of compound 7e was unambiguously confirmed via single-crystal X-ray diffraction analysis. The turbidimetric assays indicated that compounds 7b, 7d, 7g, 7k and 7n exhibited much more potent inhibition activities against the pathogen Xanthomonas oryzae pv...
September 6, 2017: Molecular Diversity
Roderich Süssmuth, Leonard von Eckardstein, Maria Seidel, John B Weston, Stefan Grätz, Monique Royer, Stephane Cociancich, Tam Dang, Daniel Petras, Souhir Sabri, Alexander Pesic, Dennis Kerwat
Natural products represent an important source of potential novel antimicrobial drug leads. Low production by microorganisms in cell culture often delays the structural elucidation or even prevents a timely discovery. Starting from the anti-Gram-negative antibacterial compound albicidin synthesized by Xanthomonas albilineans we describe a bioactivity-guided approach combined with non-targeted tandem mass spectrometry and spectral (molecular) networking for the discovery of novel antimicrobial compounds. We report eight new natural albicidin derivatives, four of which bear a β-methoxy cyanoalanine or β-methoxy asparagine as the central α-amino acid...
September 6, 2017: Chemistry: a European Journal
Zirlane Portugal da Costa, Carla de Freitas Munhoz, Maria Lucia Carneiro Vieira
BACKGROUND: Passionflowers Passiflora edulis and Passiflora alata are diploid, outcrossing and understudied fruit bearing species. In Brazil, passion fruit cultivation began relatively recently and has earned the country an outstanding position as the world's top producer of passion fruit. The fruit's main economic value lies in the production of juice, an essential exotic ingredient in juice blends. Currently, crop improvement strategies, including those for underexploited tropical species, tend to incorporate molecular genetic approaches...
September 6, 2017: BMC Research Notes
F C F Vieira, A M Gonçalves, E F R Mendoza, R M Ferreira, M L M Costa, T S Balbuena, H G Sebinelli, P Ciancaglini, J M Pizauro Junior, J A Ferro
Citrus canker, caused by the Gram-negative bacterium Xanthomonas citri subsp citri (Xac), severely affects most economically important citrus varieties worldwide. A previous study showed that disruption of the ORF XAC1201 from the Xac 306 strain by transposon Tn5 decreased bacterium virulence in the Rangpur lime host (Citrus limonia L. Osbeck). However, little is known regarding the possible function of the hypothetical protein XAC1201 and how it affects the virulence of Xac 306. Here, we confirmed that disruption of ORF XAC1201 reduces Xac 306 virulence in two different hosts, delaying the onset of typical symptoms...
August 31, 2017: Genetics and Molecular Research: GMR
James K Christenson, Serina L Robinson, Tiffany A Engel, Jack Eugene Richman, An N Kim, Lawrence P Wackett
OleB is an α/β-hydrolase found in bacteria that biosynthesize long-chain olefinic hydrocarbons, but its function has remained obscure. We report that OleB from the gram-negative bacterium Xanthomonas campestris performs an unprecedented β-lactone decarboxylation reaction, to complete cis-olefin biosynthesis. OleB reactions monitored by (1)H-NMR spectroscopy revealed a selectivity for decarboxylating cis-β-lactones and no discernable activity with trans-β-lactones, consistent with the known configuration of pathway intermediates...
September 5, 2017: Biochemistry
Ning Li, Shutong Wei, Jing Chen, Fangfang Yang, Lingguang Kong, Cuixia Chen, Xinhua Ding, Zhaohui Chu
The GT-1 cis-element widely exists in many plant gene promoters. However, the molecular mechanism that underlies the response of the GT-1 cis-element to abiotic and biotic stresses remains elusive in rice. We previously isolated a rice short-chain peptide encoding gene, Os2H16, and demonstrated that it plays important roles in both disease resistance and drought tolerance. Here, we conducted a promoter assay of Os2H16 and identified GT-1 as an important cis-element that mediates Os2H16 expression in response to pathogen attack and osmotic stress...
September 4, 2017: Plant Biotechnology Journal
Déborah Merda, Martial Briand, Eran Bosis, Céline Rousseau, Perrine Portier, Matthieu Barret, Marie-Agnès Jacques, Marion Fischer-Le Saux
Deciphering the evolutionary history and transmission patterns of virulence determinants is necessary to understand the emergence of novel pathogens. The main virulence determinant of most pathogenic proteobacteria is the type three secretion system (T3SS). The Xanthomonas genus includes bacteria responsible for numerous epidemics in agroecosystems worldwide and represents a major threat to plant health. The main virulence factor of Xanthomonas is the Hrp2 family T3SS, however this system is not conserved in all strains and it has not been previously determined whether the distribution of T3SS in this bacterial genus has resulted from losses or independent acquisitions...
September 4, 2017: Molecular Ecology
D Garcia-Seco, M Chiapello, M Bracale, C Pesce, P Bagnaresi, E Dubois, L Moulin, C Vannini, R Koebnik
The molecular details of local plant response against Xanthomonas translucens infection is largely unknown. Moreover, there is no knowledge about effects of the pathogen on the root's transcriptome and proteome. Therefore, we investigated the global gene and protein expression changes both in leaves and roots of wheat (Triticum aestivum) 24 h post leaf infection of X. translucens. This simultaneous analysis allowed us to obtain insight into possible metabolic rearrangements in above- and belowground tissues and to identify common responses as well as specific alterations...
August 31, 2017: Scientific Reports
Haigang Ma, Jie Chen, Zhenzhen Zhang, Ling Ma, Zeyu Yang, Qinglu Zhang, Xianghua Li, Jinghua Xiao, Shiping Wang
Mitogen-activated protein kinase (MAPK) cascades, with each cascade consisting of a MAPK kinase kinase (MAPKKK), a MAPK kinase (MAPKK), and a MAPK, have important roles in different biological processes. However, the signal transduction in rice MAPK cascades remains to be elucidated. We show that the structural non-canonical MAPKK, MPKK10.2, enhances rice resistance to Xanthomonas oryzae pv. oryzicola (Xoc), which causes bacterial streak disease, and increases rice tolerance to drought stress by phosphorylating and activating two MAPKs, MPK6 and MPK3, respectively...
August 30, 2017: Plant Journal: for Cell and Molecular Biology
Mylène Ruh, Martial Briand, Sophie Bonneau, Marie-Agnès Jacques, Nicolas W G Chen
BACKGROUND: Common bacterial blight is a devastating bacterial disease of common bean (Phaseolus vulgaris) caused by Xanthomonas citri pv. fuscans and Xanthomonas phaseoli pv. phaseoli. These phylogenetically distant strains are able to cause similar symptoms on common bean, suggesting that they have acquired common genetic determinants of adaptation to common bean. Transcription Activator-Like (TAL) effectors are bacterial type III effectors that are able to induce the expression of host genes to promote infection or resistance...
August 30, 2017: BMC Genomics
Hoa Thi Nguyen, Nan Hee Yu, Ae Ran Park, Hae Woong Park, In Son Kim, Jin-Cheol Kim
This study aimed to isolate and characterize an antibacterial metabolite from Pharbitis nil seeds and investigate antibacterial activity against various plant pathogenic bacteria. The methanol extract of P. nil seeds showed the strongest activity against Xanthomonas arboricola pv. pruni (Xap) with minimum inhibition concentration (MIC) value of 250 μg/ml. Among the three solvent layers obtained from the methanol extract of P. nil seeds, only butanol layer displayed the activity with MIC value of 125 μg/ml against Xap...
August 31, 2017: Journal of Microbiology and Biotechnology
Nargues Falahi Charkhabi, Nicholas J Booher, Zhao Peng, Li Wang, Heshmat Rahimian, Masoud Shams-Bakhsh, Zhaohui Liu, Sanzhen Liu, Frank F White, Adam J Bogdanove
Bacterial leaf streak caused by Xanthomonas translucens pv. undulosa (Xtu) is an important disease of wheat (Triticum aestivum) and barley (Hordeum vulgare) worldwide. Transcription activator-like effectors (TALEs) play determinative roles in many of the plant diseases caused by the different species and pathovars of Xanthomonas, but their role in this disease has not been characterized. ICMP11055 is a highly virulent Xtu strain from Iran. The aim of this study was to better understand genetic diversity of Xtu and to assess the role of TALEs in bacterial leaf streak of wheat by comparing the genome of this strain to the recently completely sequenced genome of a U...
2017: Frontiers in Microbiology
Joseph Roberts, Bangya Ma, Lane Tredway, Dave Ritchie, Jim Kerns
Bacterial etiolation and decline has developed into a widespread issue with creeping bentgrass [Agrostis stolonifera L. (CBG)] putting green turf. The condition is characterized by an abnormal elongation of turfgrass stems and leaves that in rare cases progresses into a rapid and widespread necrosis and decline. Recent reports have cited bacteria, Acidovorax avenae and Xanthomonas translucens, as causal agents; however, few cases exist where either bacterium were isolated in conjunction with turf exhibiting bacterial disease symptoms...
August 28, 2017: Phytopathology
Pei-Yi Wang, He-Shu Fang, Wu-Bin Shao, Jian Zhou, Zhuo Chen, Bao-An Song, Song Yang
Various pyridinium-functionalized carbazole derivatives were constructed by coupling the key fragments of carbazole skeleton and pyridinium nucleus in a single molecular architecture. Antibacterial bioassays revealed that some of the title compounds displayed impressive bioactivities against plant pathogens such as Xanthomonas oryzae pv. oryzae, Ralstonia solanacearum, and Xanthomonas axonopodis pv. citri with minimal EC50 values of up to 0.4, 0.3, and 0.3mg/L, respectively. These bioactivities were achieved by systematically tuning and optimizing bridging linker, alkyl length of the tailor, and substituents on the carbazole scaffold...
September 15, 2017: Bioorganic & Medicinal Chemistry Letters
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