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Genki Yoshikawa, Ahmed Askora, Romain Blanc-Mathieu, Takeru Kawasaki, Yanze Li, Miyako Nakano, Hiroyuki Ogata, Takashi Yamada
Xanthomonas virus (phage) XacN1 is a novel jumbo myovirus infecting Xanthomonas citri, the causative agent of Asian citrus canker. Its linear 384,670 bp double-stranded DNA genome encodes 592 proteins and presents the longest (66 kbp) direct terminal repeats (DTRs) among sequenced viral genomes. The DTRs harbor 56 tRNA genes, which correspond to all 20 amino acids and represent the largest number of tRNA genes reported in a viral genome. Codon usage analysis revealed a propensity for the phage encoded tRNAs to target codons that are highly used by the phage but less frequently by its host...
March 14, 2018: Scientific Reports
Jelena Purić, Gabrielle Vieira, Lúcia Bonci Cavalca, Lara Durães Sette, Henrique Ferreira, Maria Lúcia Carneiro Vieira, Daiane Cristina Sass
This study aims to obtain secondary metabolites extracts from filamentous fungi isolated from soil and marine sediments from Antarctic ecosystems and assess its potential antibacterial activity on Xanthomonas euvesicatoria and Xanthomonas axonopodis pv. passiflorae (phytopathogenic bacteria causing diseases in pepper and tomato, and passionfruit, respectively). Among the 66 crude intracellular and extracellular extracts obtained from fungi recovered from soil and 79 obtained from marine sediment samples, 25 showed the ability to prevent the growth of X...
March 12, 2018: Letters in Applied Microbiology
Abigail Savietto, Carlos Roberto Polaquini, Malgorzata Kopacz, Dirk-Jan Scheffers, Beatriz Carvalho Marques, Luís Octavio Regasini, Henrique Ferreira
Asiatic citrus canker (ACC) is an incurable disease of citrus plants caused by the Gram-negative bacterium Xanthomonas citri subsp. citri (X. citri). It affects all the commercially important citrus varieties in the major orange producing areas around the world. Control of the pathogen requires recurrent sprays of copper formulations that accumulate in soil and water reservoirs. Here, we describe the improvement of the alkyl gallates, which are potent anti-X. citri compounds, intended to be used as alternatives to copper in the control of ACC...
March 10, 2018: Archives of Microbiology
Yu Yan, Xinyi He, Wei Hu, Guoyin Liu, Peng Wang, Chaozu He, Haitao Shi
MeCIPK23 interacts with MeCBL1/9, and they confer improved defense response, providing potential genes for further genetic breeding in cassava. Cassava (Manihot esculenta) is an important food crop in tropical area, but its production is largely affected by cassava bacterial blight. However, the information of defense-related genes in cassava is very limited. Calcium ions play essential roles in plant development and stress signaling pathways. Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) are crucial components of calcium signals...
March 9, 2018: Plant Cell Reports
Chunxia Li, Shiyao Liu, Peiyi Liu, Yaxin Wang, Chunli Xu, Jun Tao, Chaozu He
Riboswitches are RNA elements that sense metabolites and control gene expression. Recently, the yybP-ykoY riboswitches were found to sense manganese (Mn2+) and regulate the expression of diverse genes. Here, we show that the leader RNA (a yybP-ykoY RNA) of yebN in Xanthomonas oryzae pv. oryzae (Xoo) also functions as a sensor of Mn2+. This leader RNA detects Mn2+ levels in plants and is essential to Xoo virulence. Our data also indicate that Mn2+ is not only required as a microelement for plant growth but also acts as a defense molecule to inhibit pathogen growth...
March 9, 2018: Molecular Plant-microbe Interactions: MPMI
Jinhong Kan, Lin An, Yao Wu, Jia Long, Liyang Song, Rongxiang Fang, Yantao Jia
During plant-pathogen interactions, pathogenic bacteria have evolved multiple strategies to cope with the sophisticated defense systems of host plants. Proline iminopeptidase (PIP) is essential to Xanthomonas campestris pv. campestris (Xcc) virulence, and conserved in many plant-associated bacteria, but its pathogenic mechanism remains unclear. In this study, we found that disrupting pip in Xcc enhanced its flagella-mediated bacterial motility by decreasing intracellular c-di-GMP levels, and overexpression of pip restricted bacterial motility by elevating c-di-GMP levels...
March 8, 2018: Molecular Plant Pathology
Rongmei Li, Shuo Wang, Ronghua Sun, Xiang He, Yongting Liu, Congfeng Song
Transcription activator-like (TAL) effectors encoded by tal genes were recognized as a key virulence strategy used by Xanthomonas oryzae pv. oryzae (Xoo) to cause bacterial leaf blight of rice. TAL effector PthXo3 is a major virulence factor identified in a Philippine Xoo strain PXO61, and it can induce the expression of susceptibility gene OsSWEET14 by binding to the effector-binding element (EBE) in the promoter region. In this study, pthXo3 homologous genes were also identified and isolated from Xoo Chinese strain OS198 and Japanese strain JXOV, which were named as PthXo3OS198 and PthXo3JXOV respectively...
March 4, 2018: FEMS Microbiology Letters
Gerard Morales, Concepció Moragrega, Emilio Montesinos, Isidre Llorente
Xanthomonas arboricola pv. pruni is the causal agent of bacterial spot disease of stone fruits and almond. The bacterium is distributed throughout the major stone-fruit-producing areas of the World and is considered a quarantine organism in the European Union according to the Council Directive 2000/29/EC, and by the European and Mediterranean Plant Protection Organization. The effect of leaf wetness duration and temperature on infection of Prunus by X. arboricola pv. pruni was determined in controlled environment experiments...
2018: PloS One
Yu-Fan Liu, Chao-Tsai Liao, Ying-Chuan Chiang, Chih-En Li, Yi-Min Hsiao
Xanthomonas campestris pv. campestris (Xcc) is the causative agent of black rot in crucifers. Here, one EZ-Tn5 transposon mutant of Xcc, altered in bacterial attachment, was isolated. Further analysis revealed that the transposon was inserted in the wxcX gene (encodes a hypothetical protein) of the transposon mutant. Sequence analysis revealed that WxcX is highly conserved in Xanthomonas, but none has been characterized. In this study, it was indicated that mutation of wxcX resulted in enhanced bacterial attachment, reduced virulence on the host cabbage, and increased sensitivity to sodium dodecyl sulfate...
March 5, 2018: Journal of Basic Microbiology
Pei Li, De-Yu Hu, Dandan Xie, Jixiang Chen, Linhong Jin, Song Baoan
This study aimed to synthesize some new sulfone derivatives containing a 1,3,4-oxadiazole moiety and investigate their in vitro antibacterial activities against Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas axonopodis pv. citri (Xac), the pathogens of rice bacterial leaf blight and citrus canker, respectively, by performing turbidimeter tests. Antibacterial-bioassay results showed that compound 6d revealed excellent bioactivities against Xoo and Xac, with the 50% effective concentration (EC50) values of 0...
March 5, 2018: Journal of Agricultural and Food Chemistry
Rezwan Tariq, Chunlian Wang, Tengfei Qin, Feifei Xu, Yongchao Tang, Ying Gao, Zhiyuan Ji, Kaijun Zhao
Bacterial blight, caused by Xanthomonas oryzae pv. oryzae ( Xoo ), is an overwhelming disease in rice-growing regions worldwide. Our previous studies revealed that the executor R gene Xa23 confers broad-spectrum disease resistance to all naturally occurring biotypes of Xoo . In this study, comparative transcriptomic profiling of two near-isogenic lines (NILs), CBB23 (harboring Xa23 ) and JG30 (without Xa23 ), before and after infection of the Xoo strain, PXO99A , was done by RNA sequencing, to identify genes associated with the resistance...
March 2, 2018: International Journal of Molecular Sciences
Ethel Bayer-Santos, Lidia Dos Passos Lima, Lucas de Moraes Ceseti, Camila Yuri Ratagami, Eliane Silva de Santana, Aline Maria da Silva, Chuck Shaker Farah, Cristina Elisa Alvarez-Martinez
Plant-associated bacteria of the genus Xanthomonas cause disease in a wide range of economically important crops. However, their ability to persist in the environment is still poorly understood. Predation by amoebas represent a major selective pressure to bacterial populations in the environment. In this study, we show that the X. citri type 6 secretion system (T6SS) promotes resistance to predation by the soil amoeba Dictyostelium discoideum. We found that an extracytoplasmic function (ECF) sigma factor (EcfK) is required for induction of T6SS genes during interaction with Dictyostelium...
February 28, 2018: Environmental Microbiology
Shiri Topman, Dafna Tamir-Ariel, Heli Bochnic-Tamir, Tal Stern Bauer, Sharoni Shafir, Saul Burdman, Zvi Hayouka
Many types of crops are severely affected by at least one important bacterial disease. Chemical control of bacterial plant diseases in the field vastly relies on copper-based bactericides, yet with limited efficacy. In this study, we explored the potential of two random peptide mixture (RPM) models as novel crop protection agents. These unique peptide mixtures consist of random combination of l-phenylalanine and l- or d-lysine (FK-20 and FdK-20, respectively) along the 20 mer chain length of the peptides. Both RPMs displayed powerful bacteriostatic and bactericidal activities towards strains belonging to several plant pathogenic bacterial genera, for example, Xanthomonas, Clavibacter and Pseudomonas...
February 28, 2018: Microbial Biotechnology
Migyeong Jeong, Iktae Kim, Gowoon Kim, Donghyun Ka, Nak-Kyun Kim, Euiyoung Bae, Kyoung-Seok Ryu, Jeong-Yong Suh
No abstract text is available yet for this article.
February 2018: FEBS Letters
Xiayan Pan, Shu Xu, Jian Wu, Jianying Luo, Yabing Duan, Jianxin Wang, Feng Zhang, Mingguo Zhou
Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight (BLB) and can be effectively controlled by phenazine-1-carboxylic acid (PCA), an antibiotic secreted by Pseudomonas spp. PCA resistance in Xoo was investigated in this research. Only four PCA-resistant strains were obtained by extensive screening, and the resistance was genetically stable in only one of them (P4). P4 was also resistant to phenazine and 1-hydroxyphezine but not to captan, bismerthiazol, or streptomycin. The following were reduced in P4 relative to the parental wild type: growth, virulence, EPS production, extracellular cellulase production and activity, biofilm formation, and swimming ability...
February 2018: Pesticide Biochemistry and Physiology
Pirjo Koivusaari, Johanna Pohjanen, Piippa R Wäli, Saija H K Ahonen, Karita Saravesi, Anna Mari Markkola, Kaisa Haapala, Marko Suokas, Janne J Koskimäki, Mysore V Tejesvi, Anna Maria Pirttilä
Plant meristems were previously thought to be sterile. Today, meristem-associated shoot endophytes are mainly reported as contaminants from plant tissue cultures, the number of observed species being very low. However, the few strains characterized have the capacity for infecting host cells and affecting plant growth and development. Here we studied the communities of endophytic bacteria in the buds of mountain birch (Betula pubescens ssp. czerepanovii (N. I. Orlova) Hämet-Ahti) exposed to winter moth (Operophtera brumata L...
February 22, 2018: Tree Physiology
Benzhong Fu, Qianqian Chen, Mi Wei, Jieqian Zhu, Lulu Zou, Guoyuan Li, Lihua Wang
Here, we report the complete genome sequence of Xanthomonas arboricola pv. juglandis DW3F3, a strong pathogenic strain isolated from blighted walnut immature fruit ( Juglans regia L. cv. Qingxiang). The genome consists of a single chromosome (5,144 kb).
February 22, 2018: Genome Announcements
Shuo Duan, Hongge Jia, Zhiqian Pang, Doron Teper, Frank White, Jeffrey Jones, Changyong Zhou, Nian Wang
Xanthomonas citri ssp. citri (Xcc) is an important plant pathogenic bacterium that causes citrus canker disease worldwide. PthA, a transcriptional activator-like (TAL) effector, directs the expression of canker susceptibility gene CsLOB1. Here, we report our recent progress in functional characterization of CsLOB1. Subcellular localization analysis of CsLOB1 protein in citrus protoplast revealed that CsLOB1 is primarily localized in the nucleus. We showed that CsLOB1 expression driven by the dexamethasone (DEX) in the CsLOB1-GR transgenic plants is associated with pustule formation following treatment with DEX...
February 20, 2018: Molecular Plant Pathology
Agata Motyka, Anna Dzimitrowicz, Piotr Jamroz, Ewa Lojkowska, Wojciech Sledz, Pawel Pohl
Diseases caused by phytopathogenic bacteria are responsible for significant economic losses, and these bacteria spread through diverse pathways including waterways and industrial wastes. It is therefore of interest to develop potent methods for their eradication. Here, antibacterial properties of direct current atmospheric pressure glow discharge (dc-APGD) generated in contact with flowing bacterial suspensions were examined against five species of phytopathogens. Complete eradication of Clavibacter michiganensis subsp...
February 19, 2018: Biotechnology and Bioengineering
Jiaming Mi, Dabing Yang, Yi Chen, Jiefeng Jiang, Haipeng Mou, Junbin Huang, Yidan Ouyang, Tongmin Mou
BACKGROUND: Breeding two-line hybrid rice with disease resistance is an effective approach to stabilize rice yield in commercial rice production of China. RESULTS: We improved the blast and bacterial blight resistance of Guangzhan63-4S, an elite photoperiod- and thermo-sensitive male sterile (P/TGMS) line widely used in two-line hybrid rice, by introducing the R genes Pi2 and Xa7 conferring resistance to rice blast and bacterial blight, respectively. Through the backcrossing and gene pyramiding breeding coupled with molecular marker-assisted selection, a new P/TGMS line Hua1228S carrying Pi2, Xa7, and tms5 was developed...
February 17, 2018: Rice
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