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Xiaolin Li, Wen Liu, Bing Li, Guoyin Liu, Yunxie Wei, Chaozu He, Haitao Shi
Gibberellin (GA) is an essential plant hormone in plant growth and development as well as various stress responses. DELLA proteins are important repressors of GA signal pathway. GA and DELLA have been extensively investigated in several model plants. However, the in vivo roles of GA and DELLA in cassava, one of the most important crops and energy crops in the tropical area, are unknown. In this study, systematic genome-wide analysis identified 4 MeDELLAs in cassava, as evidenced by the evolutionary tree, gene structures and motifs analyses...
December 26, 2017: Plant Physiology and Biochemistry: PPB
Suji Hong, Donghyun Ka, Seo Jeong Yoon, Nayoung Suh, Migyeong Jeong, Jeong-Yong Suh, Euiyoung Bae
Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins provide microbial adaptive immunity against bacteriophages. In type I-F CRISPR-Cas systems, multiple Cas proteins (Csy1-4) comprise a surveillance complex (Csy complex) with CRISPR RNA (crRNA) for target recognition. Here, we report the biochemical characterization of the Csy1-Csy2 subcomplex from Xanthomonas albilineans, including the analysis of its interaction with crRNA and also AcrF2, an anti-CRISPR (Acr) protein from a phage that infects Pseudomonas aeruginosa The X...
January 18, 2018: Journal of Biological Chemistry
Ping Cui, Rui-Fang Li, Da-Pei Zhang, Ji-Liang Tang, Guang-Tao Lu
The ability of the bacterial phytopathogen Xanthomonas campestris pv. campestris (Xcc) to cause disease is dependent on the type III secretion system (T3SS). Proteins of the Xcc T3SS are encoded by hrp (hypersensitive response and pathogenicity) genes and whose expression is mainly controlled by the regulators HrpG and HrpX. Here, we describe the identification and characterization of a previously unknown regulatory protein (named HpaP), which plays important role in hrp gene expression and virulence in Xcc...
January 18, 2018: Environmental Microbiology
Amit Levy, Choaa El-Mochtar, Chunxia Wang, Michael Goodin, Vladimir Orbovic
Background: Transient gene expression is a powerful tool to study gene function in plants. In citrus, Agrobacterium transformation is the method of choice for transient expression studies, but this method does not work efficiently with many gene constructs, and there is a need for a more robust transient expression system in citrus leaves. Biolistic particle delivery is an alternative to Agrobacterium transformation, and in some plants, such as Arabidopsis, gives higher transformation rates in leaf tissues than Agrobacterium...
2018: Plant Methods
Ling Ma, Qiang Wang, Meng Yuan, Tingting Zou, Ping Yin, Shiping Wang
The Xanthomonas genus includes Gram-negative plant-pathogenic bacteria, which infect a broad range of crops and wild plant species, cause symptoms with leaf blights, streaks, spots, stripes, necrosis, wilt, cankers and gummosis on leaves, stems and fruits in a wide variety of plants via injecting their effector proteins into the host cell during infection. Among these virulent effectors, transcription activator-like effectors (TALEs) interact with the γ subunit of host transcription factor IIA (TFIIAγ) to activate the transcription of host disease susceptibility genes...
January 10, 2018: Biochemical and Biophysical Research Communications
Heyoung Lee, Jooyoung Cha, Changhyun Choi, Naeyoung Choi, Hyun-So Ji, Sang Ryeol Park, Seungbum Lee, Duk-Ju Hwang
BACKGROUND: Plants are frequently subjected to abiotic and biotic stresses, and WRKY proteins play a pivotal role in the response to such stress. OsWRKY11 is induced by pathogens, drought, and heat, suggesting a function in biotic and abiotic stress responses. RESULTS: This study identified OsWRKY11, a member of WRKY group IIc. It is a transcriptional activator that localized to the nucleus. Ectopic expression of OsWRKY11 resulted in enhanced resistance to a bacterial pathogen, Xanthomonas oryzae pv...
January 12, 2018: Rice
Siou-Luan He, Jian-Zhi Jiang, Bo-Hong Chen, Chun-Hsiang Kuo, Shin-Lon Ho
The rice pathogenesis-related protein OsPR10a was scarcely expressed in OsCDPK1-silenced (Ri-1) rice, which was highly sensitive to pathogen infection. After inoculating the leaves with bacterial blight (Xanthomonas oryzae pv. oryzae; Xoo), we found that the expression of OsPR10a was up- and down-regulated in OEtr-1 (overexpression of the constitutively active truncated form of OsCDPK1) and Ri-1 rice plants, respectively. OsPR10a and OsCDPK1 showed corresponding expression patterns and were up-regulated in response to the jasmonic acid, salicylic acid and Xoo treatments, and OsPR1 and OsPR4 were significantly up-regulated in OEtr-1...
January 10, 2018: Scientific Reports
María Tortosa, María E Cartea, Víctor M Rodríguez, Pablo Velasco
BACKGROUND: Brassica crops together with cereals represent the basis of world supplies. Due to their importance, the production losses caused by Xanthomonas campestris pv. campestris (Xcc) infection represent a high economic impact. Understanding molecular and biochemical mechanisms of plants is essential to develop resistant crops with durable protection against diseases. In this regard, metabolomics has emerged as a valuable technology to provide an overview of the biological status of a plant exposed to a disease...
January 9, 2018: Journal of the Science of Food and Agriculture
(no author information available yet)
No abstract text is available yet for this article.
February 2018: Molecular Plant-microbe Interactions: MPMI
(no author information available yet)
No abstract text is available yet for this article.
February 2018: Molecular Plant-microbe Interactions: MPMI
Gustavo Gabriel Martos, Alicia Mamaní, María Paula Filippone, Atilio Pedro Castagnaro, Juan Carlos Díaz Ricci
HeT (1-0-galloyl-2,3; 4,6-bis-hexahydroxydiphenoyl-β-D-glucopyranose) is a penta-esterified ellagitannin obtained from strawberry leaves. Previous studies have shown that foliar application of HeT prior to inoculation with a virulent pathogen increases the resistance toward Colletotrichum acutatum in strawberry plants and to Xanthomonas citri subsp. citri in lemon plants. In this work we report that HeT induces an immediate leak of electrolytes, the hyperpolarization of the cellular membrane, a rapid Ca2+ influx to the cytoplasm during the first few seconds, which in turn modulates the accumulation of nitric oxide 5 min after treatment...
December 27, 2017: Plant Physiology and Biochemistry: PPB
Alberto M Gochez, Jose C Huguet-Tapia, Gerald V Minsavage, Deepak Shantaraj, Neha Jalan, Annett Strauß, Thomas Lahaye, Nian Wang, Blanca I Canteros, Jeffrey B Jones, Neha Potnis
BACKGROUND: Xanthomonas citri, a causal agent of citrus canker, has been a well-studied model system due to recent availability of whole genome sequences of multiple strains from different geographical regions. Major limitations in our understanding of the evolution of pathogenicity factors in X. citri strains sequenced by short-read sequencing methods have been tracking plasmid reshuffling among strains due to inability to accurately assign reads to plasmids, and analyzing repeat regions among strains...
January 4, 2018: BMC Genomics
Yue Liu, Xiaoyun Zhou, Wenbo Liu, Xiaohui Xiong, Chuyang Lv, Xiang Zhou, Weiguo Miao
HpaXm produced by the cotton leaf blight bacterium Xanthomonas citri subsp. malvacearum is a novel harpin elicitor of the induced hypersensitive response (HR) in tobacco. We investigated whether fragments of HpaXm, compared with fragments of Hpa1Xoo, are sufficient for HR or plant growth promotion (PGP) elicitation using four synthetic peptides (HpaXm35-51, HpaXm10-39, Hpa1Xoo36-52 and Hpa1Xoo10-40). We also heated the fragments to determine the heat tolerance of the functional fragments. HpaXm35-51 and Hpa1Xoo36-52 induced hypersensitive response (HR)...
2018: PloS One
Hehe Wang, Christine McTavish, William W Turechek
Xanthomonas fragariae causes angular leaf spot of strawberry, an important disease in strawberry growing regions worldwide. To better understand how X. fragariae multiplies and moves in strawberry plants, a GFP-labelled strain was constructed and used to monitor the pathogen's presence in leaf, petiole, and crown tissue with fluorescence microscopy following natural and wound inoculation in three strawberry cultivars. Taqman PCR was used to quantify bacterial densities in these same tissues regardless of the presence of GFP signal...
January 3, 2018: Phytopathology
Marta Martínez-Júlvez, Guillermina Goñi, Daniel Pérez-Amigot, Rubén Laplaza, Irina Alexandra Ionescu, Silvana Petrocelli, María Laura Tondo, Javier Sancho, Elena G Orellano, Milagros Medina
Ferredoxin-NADP(H) reductases (FNRs) deliver NADPH or low potential one-electron donors to redox-based metabolism in plastids and bacteria. Xanthomonas citri subsp. citri (Xcc) is a Gram-negative bacterium responsible for citrus canker disease that affects commercial citrus crops worldwide. The Xcc fpr gene encodes a bacterial type FNR (XccFPR) that contributes to the bacterial response to oxidative stress conditions, usually found during plant colonization. Therefore, XccFPR is relevant for the pathogen survival and its inhibition might represent a strategy to treat citrus canker...
December 24, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Jessica L Erickson, Norman Adlung, Christina Lampe, Ulla Bonas, Martin H Schattat
Xanthomonas campestris pv. vesicatoria type III secreted effectors were screened for candidates influencing plant cell processes relevant to the formation and maintenance of stromules in Nicotiana benthamiana lower leaf epidermis. Transient expression of XopL, a unique type of E3 ubiquitin ligase, led to a nearly complete elimination of stromules and the relocation of plastids to the nucleus. Further characterization of XopL revealed that the E3 ligase activity is essential for the two plastid phenotypes. In contrast to XopL wild type, a mutant XopL lacking E3 ligase activity specifically localized to microtubules...
December 29, 2017: Plant Journal: for Cell and Molecular Biology
Xiaolin Li, Shuhong Fan, Wei Hu, Guoyin Liu, Yunxie Wei, Chaozu He, Haitao Shi
Basic domain-leucine zipper (bZIP) transcription factor, one type of conserved gene family, plays an important role in plant development and stress responses. Although 77 MebZIPs have been genome-wide identified in cassava, their in vivo roles remain unknown. In this study, we analyzed the expression pattern and the function of two MebZIPs (MebZIP3 and MebZIP5) in response to pathogen infection. Gene expression analysis indicated that MebZIP3 and MebZIP5 were commonly regulated by flg22, Xanthomonas axonopodis pv...
2017: Frontiers in Plant Science
Akanksha Singh, Rupali Gupta, Sudeep Tandon, Prateeksha, Rakesh Pandey
The present investigation for the first time explains the anti biofilm and anti virulence potential of Kaffir lime oil (KLO) and its major constituent, Citronellal (3,7-dimethyloct-6-enal) against Xanthomonas oryzae pv. oryzae, causal organism of bacterial blight disease of rice. KLO at 500 ppm showed potential activity against X. oryzae pv. oryzae. Among the major components identified, citronellal (CIT) at 75 μM concentration was found to significantly inhibit biofilm along with the swimming and swarming potential of X...
December 19, 2017: Microbial Pathogenesis
Marco Bürger, Björn C Willige, Joanne Chory
Several Pseudomonas and Xanthomonas species are plant pathogens that infect the model organism Arabidopsis thaliana and important crops such as Brassica. Resistant plants contain the infection by rapid cell death of the infected area through the hypersensitive response (HR). A family of highly related α/β hydrolases is involved in diverse processes in all domains of life. Functional details of their catalytic machinery, however, remained unclear. We report the crystal structures of α/β hydrolases representing two different clades of the family, including the protein SOBER1, which suppresses AvrBsT-incited HR in Arabidopsis...
December 19, 2017: Nature Communications
Panpan Su, Wei Zhi Song, Guichun Wu, Yancun Zhao, Yuqiang Zhang, Bo Wang, Guoliang Qian, Zheng Qing Fu, Fenquan Liu
Xanthomonas oryzae pv. oryzicola (Xoc) is a X. oryzae pathovar that causes bacterial leaf streak in rice. In this study, we performed functional characterization of a nine-gene his operon in Xoc. Sequence analysis indicates that this operon is highly conserved in Xanthomonas spp. Auxotrophic assays confirmed that the his operon was involved in histidine biosynthesis. We found that two genes within this operon -trpR and hisB- were required for virulence and bacterial growth in planta. Further research revealed that trpR and hisB play different roles in Xoc...
December 19, 2017: Phytopathology
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