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Qingchun Shi, Vicente J Febres, Shujian Zhang, Fahong Yu, Greg McCollum, David G Hall, Gloria A Moore, Ed Stover
The 22-amino acid (flg22) pathogen-associated molecular pattern from the flagellin of Xanthomonas citri subsp. citri has been shown to induce defense responses correlated with citrus canker resistance. Here, flg22 of 'Candidatus Liberibacter asiaticus', the putative causal agent of Huanglongbing (HLB), elicited differential defense responses that were weaker than those from Xcc-flg22, between those of the HLB-tolerant mandarin cultivar Sun Chu Sha and susceptible grapefruit cultivar Duncan. Transcriptomics was used to compare the effect of CLas-flg22 and Xcc-flg22 between the citrus genotypes and identified 86 genes induced only by CLas-flg22 in the tolerant mandarin...
November 17, 2017: Molecular Plant-microbe Interactions: MPMI
Nelson B Lima, Marco A S Gama, Rosa L R Mariano, Wilson J Silva, Antônio R G Farias, Raul M Falcão, Lucas C Sousa-Paula, Ana M Benko-Iseppon, Sérgio S L Paiva, Valdir Q Balbino, Elineide B Souza
Here, we report the complete 5.3-Mb genome sequence of Xanthomonas campestris pv. viticola (CCRMXCV 80), which causes grapevine (Vitis vinifera L.) bacterial canker. Genome data will improve our understanding of the strain's comparative genomics and epidemiology, and help to further define plant protection and quarantine procedures.
November 16, 2017: Genome Announcements
Alvaro L Pérez-Quintero, Léo Lamy, Carlos A Zarate, Sébastien Cunnac, Erin L Doyle, Adam J Bogdanove, Boris Szurek, Alexis Derepeer
Transcription activator-like effectors (TALEs) are proteins found in the genus Xanthomonas of phytopathogenic bacteria. These proteins enter the nucleus of cells in the host plant and can induce the expression of susceptibility S genes, triggering disease. TALEs bind the promoter region of S genes following a specific code, which allows the prediction of binding sites based on TALEs' amino acid sequence. New candidate susceptibility genes can then be discovered by finding the intersection between genes induced in the presence of TALEs and genes containing predicted effector binding elements (EBEs)...
November 16, 2017: Molecular Plant-microbe Interactions: MPMI
Huiyong Xu, Ruping Wang, Yangyang Zhao, Zheng Qing Fu, Guoliang Qian, Fengquan Liu
BACKGROUND: Heat-stable antifungal factor (HSAF) is a polycyclic tetramate macrolactam secondary metabolite that exhibits broad-spectrum inhibitory activities against filamentous fungal pathogens. The native yield of this chemical is low. It is also a great challenge to synthesize HSAF artificially, due to its complex structure. Understanding the regulatory mechanism underlying HSAF biosynthesis could provide genetic basis for engineering high HSAF-producing strain. The transcription factor Clp is a global regulator that controls bacterial pathogenicity and the expression of one hundred related genes in the phytopathogenic bacterium Xanthomonas campestris pv...
November 14, 2017: Microbial Cell Factories
Kaleem U Kakar, Zarqa Nawaz, Khadija Kakar, Essa Ali, Abdulwareth A Almoneafy, Raqeeb Ullah, Xue-Liang Ren, Qing-Yao Shu
BACKGROUND: The cyclic nucleotide-gated ion channel (CNGC) family affects the uptake of cations, growth, pathogen defence, and thermotolerance in plants. However, the systematic identification, origin and function of this gene family has not been performed in Brassica oleracea, an important vegetable crop and genomic model organism. RESULTS: In present study, we identified 26 CNGC genes in B. oleracea genome, which are non-randomly localized on eight chromosomes, and classified into four major (I-IV) and two sub-groups (i...
November 13, 2017: BMC Genomics
Shulamit Manulis-Sasson, Laura Chalupowicz
Cell-to-cell communication mediated by the diffusible signal factor (DSF) is a common form of gene regulation and plays an important role in virulence of many plant pathogenic bacteria including Xanthomonas spp. Here we describe several approaches to study the involvement of DSF-dependent QS system of the plant pathogenic bacteria Xanthomonas campestris pv. pelargonii (Xhp) as an example of the Xanthomonas spp. The methods described include detection and measurement of DSF, movement in planta, colonization, and aggregate formation...
2018: Methods in Molecular Biology
Abdelmonim A Ahmad, Makoto Kawabe, Ahmed Askora, Takeru Kawasaki, Makoto Fujie, Takashi Yamada
Inovirus XacF1 (7325 nucleotides) is integrated into the genome of Xanthomonas citri pv. citri (Xcc) strains at the host dif site (attB) by the host XerC/D recombination system. The XacF1 attP sequence is located within the coding region of ORF12, a possible phage regulator. After integration, this open reading frame (ORF) is split into two pieces on the host genome. We examined dynamic integration/excision of XacF1 in Xcc strain MAFF 301080 and found that the integration started at 4 h postinfection (p.i.) and peaked at 12 h p...
November 2017: FEBS Open Bio
Lan Yang, Shijia Ge, Jian Huang, Xiaoping Bao
A series of novel (E)-2-(4-(1H-1,2,4-triazol-1-yl)styryl)-4-(alkyl/arylmethyleneoxy)quinazoline derivatives (4a-4s) were synthesized in good to excellent yields, and their structures were fully characterized by [Formula: see text] NMR, [Formula: see text] NMR, HRMS and IR spectra. The structure of compound 4b was further confirmed via single-crystal X-ray diffraction analysis. The bioassay results indicated that compounds 4s, 4q and 4n inhibit phytopathogenic bacterium Xanthomonas axonopodis pv. citri (Xac) more potently than commercial bactericide bismerthiazol...
November 8, 2017: Molecular Diversity
Stephen P Cohen, Hongxia Liu, Cristiana T Argueso, Andy Pereira, Casiana Vera Cruz, Valerie Verdier, Jan E Leach
Plant disease is a major challenge to agriculture worldwide, and it is exacerbated by abiotic environmental factors. During some plant-pathogen interactions, heat stress allows pathogens to overcome host resistance, a phenomenon which could severely impact crop productivity considering the global warming trends associated with climate change. Despite the importance of this phenomenon, little is known about the underlying molecular mechanisms. To better understand host plant responses during simultaneous heat and pathogen stress, we conducted a transcriptomics experiment for rice plants (cultivar IRBB61) containing Xa7, a bacterial blight disease resistance (R) gene, that were infected with Xanthomonas oryzae, the bacterial blight pathogen of rice, during high temperature stress...
2017: PloS One
Christopher M Ference, Alberto M Gochez, Franklin Behlau, Nian Wang, James H Graham, Jeffrey B Jones
Taxonomic status: Bacteria; Phylum Proteobacteria; Class Gammaproteobacteria; Order Xanthomonadles; Family Xanthomonadaceae; Genus Xanthomonas; Species Xanthomonas citri subsp. citri (Xcc). Host range: Compatible hosts vary in their susceptibility to CC, with grapefruit, lime, and lemon being most susceptible, sweet orange being moderately susceptible, and kumquat and calamondin being among the least susceptible (Gottwald et al., 1993). Microbiological properties: Xcc is a rod-shaped (1.5 - 2.0 X 0.5 - 0.75 µm) gram-negative, aerobic bacterium with a single polar flagellum...
November 4, 2017: Molecular Plant Pathology
Anuj Kumar, Kummara Madhusudana Rao, Sung Soo Han
Xanthan gum is a microbial high molecular weight exo-polysaccharide produced by Xanthomonas bacteria (a Gram-negative bacteria genus that exhibits several different species) and it has widely been used as an additive in various industrial and biomedical applications such as food and food packaging, cosmetics, water-based paints, toiletries, petroleum, oil-recovery, construction and building materials, and drug delivery. Recently, it has shown great potential in issue engineering applications and a variety of modification methods have been employed to modify xanthan gum as polysaccharide for this purpose...
January 15, 2018: Carbohydrate Polymers
Xinmin Zhong, Xiaobin Wang, Lijuan Chen, Xianghui Ruan, Qin Li, Juping Zhang, Zhuo Chen, Wei Xue
BACKGROUND: Myricetin and 1,3,4-thiadiazole derivatives were reported to exhibit favorable antiviral and antibacterial activities. Aiming to discover novel myricetin analogues with potent activities, a series of novel myricetin derivatives containing 1,3,4-thiadiazole moiety were synthesized, and their antibacterial and antiviral activities were evaluated. RESULT: Bioassay results indicated that some target compounds exhibited potential antibacterial and antiviral activities...
October 17, 2017: Chemistry Central Journal
Jehan Bakht, Zainab Iftikhar, Mohammad Shafi, Arshad Iqbal
The present research was focused on the anti-microbial activities of different solvent extracted fractions from commercial available and fresh plants of Berberis lyceum against Gram positive, Gram-negative bacteria and fungi using 1 and 2 mg disc(-1) concentrations. Our results showed that fractions from both sources revealed different degree of antimicrobial activities. Our result indicated that Escherichia coli, Citorbacter freundii and Candida albicans were more susceptible to crude methanolic extract and the same microbes were resistant to water extracted fractions...
September 2017: Pakistan Journal of Pharmaceutical Sciences
Yue Pan, Fang Liang, Ru-Jiao Li, Wei Qian
MarR-family transcription factors (TFs), which regulate the expression of virulence factors and other physiological pathways in pathogenic bacteria, are regarded as ideal molecular targets for the development of novel antimicrobial strategies. In the plant bacterial pathogen Xanthomonas campestris pv. campestris, HpaR, a typical MarR-family TF, is associated with bacterial virulence, but its mechanism of virulence regulation remains unclear. Here, we dissected the HpaR regulon using high-throughput RNA sequencing and chromatin immunoprecipitation sequencing...
October 27, 2017: Molecular Plant-microbe Interactions: MPMI
Denver I Walitang, Kiyoon Kim, Munusamy Madhaiyan, Young Kee Kim, Yeongyeong Kang, Tongmin Sa
BACKGROUND: Rice (Oryza sativa L. ssp. indica) seeds as plant microbiome present both an opportunity and a challenge to colonizing bacterial community living in close association with plants. Nevertheless, the roles and activities of bacterial endophytes remain largely unexplored and insights into plant-microbe interaction are compounded by its complexity. In this study, putative functions or physiological properties associated with bacterial endophytic nature were assessed. Also, endophytic roles in plant growth and germination that may allow them to be selectively chosen by plants were also studied...
October 26, 2017: BMC Microbiology
Cristina Camó, Maria Torné, Emili Besalú, Cristina Rosés, Anna D Cirac, Gemma Moiset, Esther Badosa, Eduard Bardají, Emilio Montesinos, Marta Planas, Lidia Feliu
A library of 66 cyclic decapeptides incorporating a Trp residue was synthesized on solid phase and screened against the phytopathogenic bacteria Pseudomonas syringae pv. syringae, Xanthomonas axonopodis pv. vesicatoria, and Erwinia amylovora. The hemolytic activity of these peptides was also evaluated. The results obtained were compared with those of a collection of Phe analogues previously reported. The analysis of the data showed that the presence of the Trp improved the antibacterial activity against these three pathogens...
October 26, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Debora Liabeuf, Sung-Chur Sim, David M Francis
Bacterial spot affects tomatoes (Solanum lycopersicum L.) grown under humid conditions. Major genes and quantitative trait loci (QTL) for resistance have been described, and multiple loci from diverse sources need to be combined to improve disease control. We investigated genomic selection (GS) prediction models for resistance to Xanthomonas euvesicatoria and experimentally evaluated the accuracy of these models. The training population consisted of 109 families combining resistance from four sources, and directionally selected from a population of 1,100 individuals...
October 24, 2017: Phytopathology
Yu Yan, Peng Wang, Chaozu He, Haitao Shi
As a highly conserved mechanism, autophagy is responsible for the transport of cytoplasmic constituents in the vacuoles or lysosomes. Moreover, autophagy is essential for plant development and various stress responses. In this study, 34 MeATGs were systematically identified in cassava, and their transcripts were commonly regulated by Xanthomonas axonopodis pv manihotis (Xam). Through transient expression in Nicotiana benthamiana, the subcellular locations of 4 MeATG8s were revealed. Notably, MeWRKY20 was identified as physical interacting protein of MeATG8a/8f/8h and upstream transcriptional activator of MeATG8a...
December 9, 2017: Biochemical and Biophysical Research Communications
Guixia Hao, Shujian Zhang, Ed Stover
Citrus Huanglongbing (HLB) associated with 'Candidatus Liberibacter asiaticus' (Las) and citrus canker disease incited by Xanthomonas citri are the most devastating citrus diseases worldwide. To control citrus HLB and canker disease, we previously screened over forty antimicrobial peptides (AMPs) in vitro for their potential application in genetic engineering. D2A21 was one of the most active AMPs against X. citri, Agrobacterium tumefaciens and Sinorhizobium meliloti with low hemolysis activity. Therefore, we conducted this work to assess transgenic expression of D2A21 peptide to achieve citrus resistant to canker and HLB...
2017: PloS One
Hey-Jee Park, Nahee Bae, Hanbi Park, Dae-Wi Kim, Sang-Wook Han
Bacteria change their gene expression when exposed to different nutrient conditions. The levels of proteins do not always correlate with those of RNAs, hence proteomic analysis is required for understanding how bacteria adapt to different conditions. Herein, differentially abundant proteins from Xanthomonas oryzae pv. oryzae (Xoo), X. campestris pv. vesicatoria (Xcv), and X. axonopodis pv. glycines (Xag), which were cultured in rich media and in minimal media, were determined using label-free shotgun proteomic analysis and clusters of orthologous groups classification...
October 17, 2017: Proteomics
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