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Amniotic membrane

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https://www.readbyqxmd.com/read/27898936/nutrient-transporters-in-bovine-uteroplacental-tissues-on-days-sixteen-to-fifty-of-gestation
#1
M S Crouse, K J McLean, M R Crosswhite, L P Reynolds, C R Dahlen, B W Neville, P P Borowicz, J S Caton
During early gestation, nutrients are transported to the developing embryo via transporters in the uterine endometrium and chorioallantois. In the present study, we examined glucose transporters and and the cationic AA transporters , , and to test the hypotheses that 1) relative mRNA expression of transporters would be different among uteroplacental tissue type as gestation progresses and 2) concentrations of glucose and cationic AA would be different among target sites (placental compartments, serum, and histotrophic) and days of gestation...
November 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27897025/-nonculture-techniques-of-microorganisms-determination-in-amniotic-fluid-in-patients-with-preterm-prelabor-rupture-of-membranes
#2
R Kutová, L Plíšková, M Kacerovský
OBJECTIVE: The preterm prelabor rupture of membranes is a serious obstetric complication that is frequently complicated by the presence of microorganisms in amniotic fluid. The aim of our work is to characterize current status of nonculture detection of microbial invasion into the amniotic cavity and the experience with the technique performed in University Hospital in Hradec Kralove. DESIGN: Original survey article. SETTING: Institute of Clinical Biochemistry and Diagnostics - molecular biology department, University Hospital Hradec Kralove...
2016: Ceská Gynekologie
https://www.readbyqxmd.com/read/27894883/incubation-relative-humidity-induces-renal-morphological-and-physiological-remodeling-in-the-embryo-of-the-chicken-gallus-gallus-domesticus
#3
Greta Bolin, Benjamin Dubansky, Warren W Burggren
The metanephric kidneys of the chicken embryo, along with the chorioallantoic membrane, process water and ions to maintain osmoregulatory homeostasis. We hypothesized that changes in relative humidity (RH) and thus osmotic conditions during embryogenesis would alter the developmental trajectory of embryonic kidney function. White leghorn chicken eggs were incubated at one of 25-30% relative humidity, 55-60% relative humidity, and 85-90% relative humidity. Embryos were sampled at days 10, 12, 14, 16, and 18 to examine embryo and kidney mass, glomerular characteristics, body fluid osmolalities, hematological properties, and whole embryo oxygen consumption...
November 25, 2016: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/27889918/inner-ear-labyrinth-anatomy-of-monotremes-and-implications-for-mammalian-inner-ear-evolution
#4
Julia A Schultz, Ulrich Zeller, Zhe-Xi Luo
The monophyletic clade Monotremata branches early from the rest of the mammalian crown group in the Jurassic and members of this clade retain many ancestral mammalian traits. Thus, accurate and detailed anatomical descriptions of this group can offer unique insight into the early evolutionary history of Mammalia. In this study, we examine the inner ear anatomy of two extant monotremes, Ornithorhynchus anatinus and Tachyglossus aculeatus, with the primary goals of elucidating the ancestral mammalian ear morphology and resolving inconsistencies found within previous descriptive literature...
November 27, 2016: Journal of Morphology
https://www.readbyqxmd.com/read/27888650/closure-of-the-cervical-os-in-patients-after-fertility-preserving-treatment-for-early-cervical-cancer-results-of-a-prospective-observational-study
#5
Mandy Mangler, Malgorzata Lanowska, Anne Bartens, Aaron Schindler, Jens-Uwe Blohmer, Dorothee Speiser
INTRODUCTION: Up to 50% of the infants delivered after radical vaginal trachelectomy (RVT) are born prematurely. An effective strategy to reduce this number could be the closure of the cervical os (CCO). PATIENTS AND METHODS: Fifteen pregnant patients who had a RVT due to early cervical cancer were included in this prospective case control study. All patients were scheduled for CCO early in the second trimester. CCO was performed in 12 patients. Their data were compared to data from 125 pregnancies after a RVT without CCO...
November 26, 2016: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/27888184/amnion-assisted-conjunctival-epithelial-redirection-in-limbal-stem-cell-grafting
#6
Harminder S Dua, Ammar Miri, Mohamed S Elalfy, Anna Lencova, Dalia G Said
AIMS: To develop a technique using amniotic membrane (AM) to prevent admixture of conjunctival epithelial cells and limbal explant-derived corneal epithelial cells in patients undergoing limbal stem cell transplantation. To compare this technique with the current method of 'sequential sector conjunctival epitheliectomy' (SSCE). METHODS: 26 patients with total limbal stem cell deficiency who underwent ocular surface reconstruction with limbal stem cells transplantation were retrospectively studied...
November 25, 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/27882109/excisional-keratectomy-combined-with-focal-cryotherapy-and-amniotic-membrane-inlay-for-recalcitrant-filamentary-fungal-keratitis-a-retrospective-comparative-clinical-data-analysis
#7
Yingxin Chen, Minghong Gao, Joshua K Duncan, Di Ran, Denise J Roe, Michael W Belin, Mingwu Wang
The aim of the present study was to investigate the efficacy of a novel surgical intervention, excisional keratectomy combined with focal cryotherapy and amniotic membrane inlay (EKCAI), for the treatment of recalcitrant filamentary fungal keratitis. A retrospective analysis was performed of patients who underwent excisional keratectomy combined with conjunctival flap inlay (EKCFI), EKCAI or therapeutic penetrating keratoplasty (TPK) from January 2006 to January 2011. Recalcitrance was determined as being unresponsive to standard medical antifungal therapy for at ≥1 week...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27881591/vernal-keratoconjunctivitis-culmination-of-management-using-immunosuppression-surgical-and-prosthetic-therapy-over-quarter-century
#8
Shilpa Das, Anand S Pasari, Virender S Sangwan
A 22-year-old male patient presented in 1988 with active vernal keratoconjunctivitis. He was treated with topical mast cell stabilisers and corticosteroids. Chronic inflammation despite topical treatment necessitated oral immunosuppressants. Active disease came under control with this; however, the patient gradually developed limbal stem cell deficiency. He underwent bilateral pannus resection with amniotic membrane transplantation that resulted in improved ocular surface. In 2007, patient was found to have significant bilateral posterior subcapsular cataracts and underwent bilateral cataract surgery with intraocular lens implantation with good visual outcome...
November 23, 2016: BMJ Case Reports
https://www.readbyqxmd.com/read/27881176/no-midterm-advantages-in-the-middle-term-using-small-intestinal-submucosa-and-human-amniotic-membrane-in-achilles-tendon-transverse-tenotomy
#9
Yushu Liu, Yinbo Peng, Yong Fang, Min Yao, Robert W Redmond, Tao Ni
BACKGROUND: The study was aimed to compare the effects of small intestinal submucosa (SIS) and human amniotic membrane (HAM) on Achilles tendon healing. METHODS: A total of 48 New Zealand white rabbits were divided into two groups. A full-thickness transverse tenotomy was made at the right leg of the rabbits. Then, the laceration site was wrapped with HAM (P/A group) or SIS (P/S group). The ultimate stress (US) and Young's modulus (E) of the tendons were detected for biomechanical analysis...
November 24, 2016: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/27872513/vaginal-lactoferrin-modulates-pge2-mmp-9-mmp-2-and-timp-1-amniotic-fluid-concentrations
#10
Alessandro Trentini, Martina Maritati, Carlo Cervellati, Maria C Manfrinato, Arianna Gonelli, Carlo A Volta, Fortunato Vesce, Pantaleo Greco, Franco Dallocchio, Tiziana Bellini, Carlo Contini
Inflammation plays an important role in pregnancy, and cytokine and matrix metalloproteases (MMPs) imbalance has been associated with premature rupture of membranes and increased risk of preterm delivery. Previous studies have demonstrated that lactoferrin (LF), an iron-binding protein with anti-inflammatory properties, is able to decrease amniotic fluid (AF) levels of IL-6. Therefore, we aimed to evaluate the effect of vaginal LF administration on amniotic fluid PGE2 level and MMP-TIMP system in women undergoing genetic amniocentesis...
2016: Mediators of Inflammation
https://www.readbyqxmd.com/read/27870156/combinations-and-loads-of-bacteria-affect-the-cytokine-production-by-fetal-membranes-an-in-vitro-study
#11
Nathalia Mayumi Noda-Nicolau, Jossimara Polettini, Morgan R Peltier, Márcia Guimarães da Silva, Ramkumar Menon
PROBLEM: The polybacterial invasion and inflammation of the amniotic cavity is a common scenario in PTB, and then, we analyzed the cytokine production by human fetal membranes to better understand the host response to polybacterial infections. METHOD OF STUDY: Fetal membranes were treated by heat-inactivated genital mycoplasmas and Gardnerella vaginalis at 10(3) or 10(6) colony/color-forming units/mL alone or in combination. Cytokines/receptors were measured in the medium by immunoassays...
December 2016: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/27867342/the-family-of-crumbs-genes-and-human-disease
#12
REVIEW
Anne M Slavotinek
The family of vertebrate Crumbs proteins, homologous to Drosophila Crumbs (Crb), share large extracellular domains with epidermal growth factor-like repeats and laminin-globular domains, a single transmembrane domain, and a short intracellular C-terminus containing a single membrane proximal 4.1/ezrin/radixin/moesin-binding domain and PSD-95/Discs large/ZO-1-binding motifs. There are 3 Crb genes in humans - Crumbs homolog-1 (CRB1), Crumbs homolog-2 (CRB2), and Crumbs homolog-3 (CRB3). Bilallelic loss-of-function mutations in CRB1 cause visual impairment, with Leber's congenital amaurosis and retinitis pigmentosa, whereas CRB2 mutations are associated with raised maternal serum and amniotic fluid alpha feto-protein levels, ventriculomegaly/hydrocephalus, and renal disease, ranging from focal segmental glomerulosclerosis to congenital Finnish nephrosis...
October 2016: Molecular Syndromology
https://www.readbyqxmd.com/read/27864846/monochorial-diamniotic-dizygotic-twins-in-a-german-shepherd-dog-a-case-report
#13
C Urhausen, K Wolf, A Beineke, C Dierks, M Schmicke, A Einspanier, A R Günzel-Apel
A 6.5-year-old clinically healthy German Shepherd Dog with regular oestrous cycles of 6 months was presented for pregnancy diagnosis on day 38 after ovulation (p.ov.). Ultrasonography revealed three individual placental sites in progressed resorption and two vital adequately developed foetuses sharing a joint placenta. On days 41 and 48 p.ov., sonographic signs indicated normal development of both foetuses, but on day 52 p.ov., both foetuses were found to be dead. A caesarean section was performed the same day...
November 18, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27853330/effect-of-human-amniotic-membrane-on-prevention-of-colorectal-anastomosis-leakage-in-cases-with-neoadjuvant-radiotherapy-an-experimental-animal-study
#14
Sam Moslemi, Sajjad Ahmadi Joraghi, Reza Roshanravan, Leila Ghahramani, Mohammad Mohammadianpanah, Masood Hosseinzadeh, Abbas Rezaianzadeh, Ahmed Mohammed Ali Hussein, Neda Najibpour, Seyed Vahid Hosseini
BACKGROUND: Radiotherapy is one of the most important factors which results in negative effects on wound healing and increases anastomosis leakage. Diverting loop ileostomy has been usually performed after colorectal anastomosis in cases of colorectal cancer with a history of neoadjuvant radiotherapy to decrease the chance of leakage. Considering the side effects of diverting loop ileostomy, the objective of the present study is to investigate the effect of human amniotic membrane (HAM) on colorectal anastomosis leakage after neo-adjuvant radiotherapy...
November 2016: Iranian Journal of Medical Sciences
https://www.readbyqxmd.com/read/27852013/modulation-of-cell-attachment-proliferation-and-angiogenesis-by-decellularized-dehydrated-human-amniotic-membrane-in-in-vitro-models
#15
Xuan Guo, Aleksandr Kaplunovsky, Raihana Zaka, Chuan Wang, Hemlata Rana, Jeffrey Turner, Qian Ye, Ivana Djuretic, Joseph Gleason, Vladimir Jankovic, Janice M Smiell, Mohit Bhatia, Wolfgang Hofgartner, Robert Hariri
BACKGROUND: Decellularized, dehydrated human amniotic membrane (DDHAM) is an extracellular matrix devoid of cells, cell debris, and growth factors. This study examines the effect of cell attachment to the DDHAM and the induced cellular responses. MATERIALS AND METHODS: The cell types employed in this study were human dermal fibroblasts (HDF), human epithelial keratinocytes (HEK), and human dermal microvascular endothelial cells (HDMEC), all of which play critical roles in the wound healing process...
October 24, 2016: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/27843896/surgical-reconstruction-of-ocular-surface-tumors-using-fibrin-sealant-tissue-adhesive
#16
Aline Roseane Queiroz de Paiva, Larissa Abreu de Azevedo Fraga, Virgínia Laura Lucas Torres
PURPOSE: To evaluate the surgical outcomes of ocular surface reconstruction in corneal-conjunctival tumors using fibrin tissue adhesive. METHODS: A prospective noncomparative study was performed between May 2013 and February 2015. Patients were submitted to routine surgical procedure for corneal-conjunctival tumor excision followed by amniotic membrane graft transplantation using fibrin tissue adhesive (Evicel®, Omrix Biopharmaceuticals Ltd., Israel). Results were assessed on the 1st, 7th, 15th and 30th postoperative days to analyze subjective complaints, adhesiveness and positioning of the graft, potential complications and recurrences...
October 2016: Ocular Oncology and Pathology
https://www.readbyqxmd.com/read/27834121/demineralized-freeze-dried-bone-allograft-with-amniotic-membrane-in-the-treatment-of-periodontal-intrabony-defects-12-month-randomized-controlled-clinical-trial
#17
Deepthi D Sali, Joann Pauline George
AIM: The goal of randomized controlled clinical study was to compare the clinical and radiological outcomes of the combination of Open flap debridement, Amniotic membrane and Demineralized Freeze Dried bone Allograft with Open flap debridement and Demineralized Freeze Dried bone Allograft alone in the management of periodontal intrabony defects. METHOD: 10 patients with bilaterally similar intrabony defects were selected. Each of the intrabony defects were randomly allocated for control and test groups...
November 11, 2016: Journal of Periodontology
https://www.readbyqxmd.com/read/27832332/a-successfully-treated-case-of-cardiac-arrest-after-caesarean-section-complicated-by-pheochromocytoma-crisis-and-amniotic-fluid-embolism
#18
Kenichiro Mita, Kayo Tsugita, Yoshikazu Yasuda, Yasunari Matsuki, Yurie Obata, Yuka Matsuki, Seiichi Kamisawa, Kenji Shigemi
Both pheochromocytoma and amniotic fluid embolism (AFE) are important causes of maternal mortality. We present a case of a 29-year-old woman who developed cardiac arrest after Caesarean section, complicated by both pheochromocytoma crisis and AFE. After resuscitation, the patient developed multiple organ dysfunction, rhabdomyolysis and disseminated intravascular coagulation (DIC). After institution of multidisciplinary interventions (including the use of an intra-aortic balloon pump, extracorporeal membrane oxygenation, continuous hemodiafiltration, and neuroprotective therapeutic hypothermia) the patient made a full recovery without any apparent neurological deficit...
November 10, 2016: Journal of Anesthesia
https://www.readbyqxmd.com/read/27831903/calciprotein-particles-as-potential-etiologic-agents-of-idiopathic-preterm-birth
#19
Lydia L Shook, Catalin S Buhimschi, Antonette T Dulay, Megan E McCarthy, John T Hardy, Christina M Duzyj Buniak, Guomao Zhao, Irina A Buhimschi
Preterm birth (PTB) is a leading cause of neonatal morbidity and mortality and is often preceded by preterm premature rupture of the membranes (PPROM) without an identifiable cause. Pathological calcification, the deposition of hydroxyapatite (HA) in nonskeletal tissues, has been implicated in degenerative diseases including atherosclerosis and aneurism rupture. Among pathogenic mechanisms, the aberrant aggregation of HA into calciprotein particles (CPPs) and the HA-induced differentiation of mesenchymal cells into osteoblasts (ectopic osteogenesis) have been implicated...
November 9, 2016: Science Translational Medicine
https://www.readbyqxmd.com/read/27830445/tissue-engineering-for-neurodegenerative-diseases-using-human-amniotic-membrane-and-umbilical-cord
#20
Anahí Sanluis-Verdes, Namibia Sanluis-Verdes, María Jesús Manso-Revilla, Antonio Manuel Castro-Castro, Jorge Pombo-Otero, María Fraga-Mariño, Jacinto Sanchez-Ibañez, Nieves Doménech, María Esther Rendal-Vázquez
Regenerative medicine, based on the use of stem cells, scaffolds and growth factors, has the potential to be a good approach for restoring damaged tissues of the central nervous system. This study investigated the use of human amniotic mesenchymal stem cells (hAMSC), human amniotic epithelial stem cells (hAESC), and human Wharton's jelly mesenchymal stem cells (hWJMSC) derived from human umbilical cord as a source of stem cells, and the potential of the human amniotic membrane (HAM) as a scaffold and/or source of growth factors to promote nerve regeneration...
November 9, 2016: Cell and Tissue Banking
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