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https://www.readbyqxmd.com/read/29165417/-pterygium-etiology-pathogenesis-treatment
#1
S A Malozhen, S V Trufanov, D A Krakhmaleva
Pterygium is a degenerative condition characterized by fibrovascular outgrowth of conjunctiva over the cornea. Many theories exist that try to explain its pathogenesis. The current belief is that this disease is multifactorial with ultraviolet radiation being the most important trigger. Attention is also paid to such factors as tear film changes, cytokines and growth factors disbalance, immunologic disturbances, genetic mutations, and viral infections. Modern classifications consider the rate of fibrovascular growth, its progressive potential, and histological features...
2017: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/29159576/an-experience-of-second-trimester-anhydramnios-salvaged-by-single-amnioinfusion
#2
Shingo Io, Eiji Kondoh, Yoshitsugu Chigusa, Hirohiko Tani, Junzo Hamanishi, Ikuo Konishi
Second-trimester anhydramnios, which is primarily caused by ruptured membranes, placental dysfunction, or congenital renal malformations, is associated with high perinatal morbidity and mortality. Although amnioinfusion temporarily increases amniotic fluid volume, it does not generally provide a fundamental solution. We describe a case of second-trimester anhydramnios with an umbilical cord factor, wherein single amnioinfusion may have successfully broken a vicious circle involving non-reassuring fetal status...
November 20, 2017: Journal of Medical Ultrasonics
https://www.readbyqxmd.com/read/29146761/comparison-among-adjuvant-treatments-for-primary-pterygium-a-network-meta-analysis
#3
Ellen Carrara Fonseca, Eduardo Melani Rocha, Gustavo Viani Arruda
PURPOSE: Pterygium is a frequent ocular disease, where the major challenge is the high level of recurrence after its surgical removal. We performed a network meta-analysis to identify, among several adjuvant treatments for primary pterygium, which is the best to prevent recurrence. METHODS: A search was conducted using PubMed, Scientific Electronic Library Online, Latin American and Caribbean Centre on Health Sciences and Cochrane Eyes and Vision Group Trials Register between 1993 and 2015 for randomisedclinical trials (RCTs) comparing adjuvant treatments following primary pterygium surgery...
November 16, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29144789/a-comparative-study-of-different-amniotic-membrane-orientations-during-extraocular-muscle-surgery-in-rabbits
#4
Rehab Rashad Kassem, Randa Mohamed Abdel-Moneim El-Mofty, Mustafa Mahmoud Khodeir, Wael Mostafa Hamza
PURPOSE: To histopathologically compare the effect of different orientations of cryopreserved human amniotic membrane (AM) transplant during extraocular muscle surgery in rabbits. METHODS: Fifty-two albino rabbit eyes underwent 4-mm resection of the superior rectus. Eyes were randomly divided into four groups. In Group C (Control group, 16 eyes) the muscle was not wrapped with amniotic membrane. In the three AM groups, cryopreserved AM was wrapped around the muscle, oriented with either its stroma (Group S, 15 eyes) or epithelium (Group E, nine eyes) towards the muscle, or folded on itself with the epithelium externally (Group F, 12 eyes)...
November 16, 2017: Current Eye Research
https://www.readbyqxmd.com/read/29140861/sealing-of-corneal-lacerations-using-photoactivated-rose-bengal-dye-and-amniotic-membrane
#5
Timothy A Soeken, Hong Zhu, Sheri DeMartelaere, Brett W Davies, Mirang Kim, Heuy-Ching Wang, James Aden, Rose Grimm, Clemens Alt, Irene E Kochevar, Anthony J Johnson
PURPOSE: Watertight closure of perforating corneoscleral lacerations is necessary to prevent epithelial ingrowth, infection, and potential loss of the eye. Complex lacerations can be difficult to treat, and repair with sutures alone is often inadequate. In this study, we evaluated a potentially sutureless technology for sealing complex corneal and scleral lacerations that bonds the amniotic membrane (AM) to the wound using only green light and rose bengal dye. METHODS: The AM was impregnated with rose bengal and then sealed over lacerations using green light to bond the AM to the deepithelialized corneal surface...
November 14, 2017: Cornea
https://www.readbyqxmd.com/read/29139122/vaccination-with-human-amniotic-epithelial-cells-confer-effective-protection-in-a-murine-model-of-colon-adenocarcinoma
#6
M Tabatabaei, N Mosaffa, R Ghods, S Nikoo, S Kazemnejad, M Khanmohammadi, E Mirzadeghan, A Mahmoudi, M R Bolouri, R Falak, B Keshavarzi, M Ramezani, A H Zarnani
In spite of being the star of therapeutic strategies for cancer treatment, the results of immunotherapeutic approaches are still far from expectations. In this regard, primary prevention of cancer using prophylactic cancer vaccines has gained considerable attention. The immunologic similarities between cancer development and placentation have helped researchers to unravel molecular mechanisms responsible for carcinogenesis and to take advantage of stem cells from reproductive organs to elicit robust anti-cancer immune responses...
November 15, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29133658/role-of-amniotic-membrane-and-full-thickness-skin-graft-in-reconstruction-of-kissing-nevus-of-eyelids
#7
Manpreet Singh, Natasha Gautam, Manpreet Kaur, Zoramthara Zadeng
A 20-year-old girl presented with a large pigmented lesion over the temporal aspect of left lateral canthus involving the lateral one-third of both eyelids, since birth. A slow increase in its size had been noticed for the past 2 years. On examination, a hairy nevus measuring 34 mm × 22 mm was noticed involving left eyelid skin, the mucocutaneous junction (MCJ), palpebral conjunctiva, and lateral canthus. The surface was irregularly thick and covered with long, thick, and pigmented hairs. A clinical diagnosis of kissing nevus of eyelids was kept and a surgical resection with reconstruction planned...
November 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29133641/simultaneous-use-of-amniotic-membrane-and-mitomycin-c-in-trabeculectomy-for-primary-glaucoma
#8
Usha Yadava, Kirti Jaisingh, Sonal Dangda, Prolima Thacker, Kirti Singh, Yashpal Goel
PURPOSE: This study aimed to propose the role of amniotic membrane transplantation (AMT) as an additional modulator in primary Mitomycin C (MMC)-augmented trabeculectomy. METHODS: This was a randomized prospective interventional study. Forty eyes of 39 adult patients with uncontrolled primary glaucoma were randomly divided into two equal groups. Control group underwent trabeculectomy augmented with MMC while the study group underwent additional AMT. Patients were followed up for 12 months and outcomes measured were intraocular pressure (IOP), need for additional intervention, and bleb morphology...
November 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29128574/conjunctivochalasis-a-systematic-review
#9
REVIEW
Anna Marmalidou, Ahmad Kheirkhah, Reza Dana
Conjunctivochalasis (CCH) is a conjunctival condition characterized by loose, redundant conjunctival folds, most typically in the inferior bulbar conjunctiva of both eyes. Although CCH is a common cause of ocular irritation and discomfort, especially in the elderly, it is often overlooked in clinical practice. Conjunctivochalasis may be associated with various ocular and non-ocular conditions; however, the most important risk factor is aging. Although often asymptomatic, CCH may cause symptoms related to tear film instability and/or delayed tear clearance...
November 8, 2017: Survey of Ophthalmology
https://www.readbyqxmd.com/read/29127427/amniotic-membrane-promotes-focal-adhesion-remodeling-to-stimulate-cell-migration
#10
Ángel Bernabé-García, Sergio Liarte, Jose M Moraleda, Gregorio Castellanos, Francisco J Nicolás
During wound healing, the migration of keratinocytes onto newly restored extracellular matrix aims to reestablish continuity of the epidermis. The application of amniotic membrane (AM) to chronic, deep traumatic, non-healing wounds has proven successful at stimulating re-epithelialization. When applied on epithelial cell cultures, AM activates extracellular signal-regulated kinases 1/2 (ERK1/2) and c-Jun N-terminal kinases 1/2 (JNK1/2), with the overexpression and phosphorylation of c-Jun along the wound edge...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29125135/silk-fibroin-amniotic-membrane-3d-bi-layered-artificial-skin
#11
Mazaher Gholipourmalekabadi, A Samadikuchaksaraei, Alexander Marcus Seifalian, Aleksandra Urbanska, Hossein Ghanbarian, John George Hardy, Mir Omrani, Masoud Mozafari, Rui Luis Reis, Subhas Chandra Kundu
Burn injury has been reported to be an important cause of morbidity and mortality and it is still considered as unmet clinical need. Although there is a myriad of effective stem cells suggested for skin regeneration, there is no one ideal scaffold. The aim of this study was to develop a 3D bi-layer scaffold made of biological decellularized human amniotic membrane (AM) with viscoelastic electrospun nanofibrous silk fibroin (ESF) spun on top. The fabricated 3D bi-layer AM/ESF scaffold was submerged in ethanol to induce β-sheet transformation as well as to get a tightly coated and inseparable bilayer...
November 10, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/29124501/quality-and-clinical-outcomes-associated-with-a-gentamicin-use-system-change-for-managing-chorioamnionitis
#12
Jason B Sauberan, Brittney Choi, Alexander R Paradyse, Jennifer Le
Chorioamnionitis is an intra-amniotic infection with serious maternal and neonatal complications. Clinical studies suggest antibiotic administration before delivery reduces the risk of complications compared to after delivery. Our center implemented a standardized intrapartum gentamicin computerized provider order entry and dosage form dispensing system intended to improve treatment initiation efficiency in hospitalized obstetric patients. The primary objective of this retrospective study was to determine if these system changes were associated with decreased time from gentamicin ordering to administration in patients with chorioamnionitis...
November 9, 2017: Journal of Medical Systems
https://www.readbyqxmd.com/read/29114268/vaginal-fluid-urea-and-creatinine-in-the-diagnosis-of-premature-rupture-of-membranes-in-resource-limited-community-settings
#13
Jasmina Begum, Sunil Kumar Samal, Seetesh Ghose, Gopal Niranjan
Objective: Diagnosis of premature rupture of membranes (PROM) is difficult in equivocal cases with traditional methods. This study aimed to evaluate the reliability of vaginal washing fluid urea and creatinine for diagnosis of PROM and to determine the cut off value. Materials and methods: The current study was a prospective case control. Women having gestational age of 28 to 42 weeks were divided into two equal groups: Fifty with history of leaking per vagina (study group) and an equal number with gestation matched none leaking (control group) were recruited...
March 2017: Journal of Family & Reproductive Health
https://www.readbyqxmd.com/read/29111829/effect-of-cryopreserved-amniotic-membrane-on-the-mechanical-properties-of-skeletal-muscle-after-strabismus-surgery-in-rabbits
#14
Marta Sierra, Ángel Ortillés, Francisco J Miana-Mena, Jorge Grasa, Begoña Calvo
PURPOSE: To study the functional recovery of the superior rectus muscle (SRM) after its partial resection in a rabbit model with and without cryopreserved amniotic membrane (AM). MATERIAL AND METHODS: Resection of the right and left SRMs of 30 rabbits was performed. On the left eyes, a single sheet of equine cryopreserved AM was placed covering the muscle edge sutured. Active and passive mechanical properties of muscles operated with and without AM were monitored over time at 30 (n = 10), 60 (n = 10), and 90 (n = 10) days after surgery...
November 7, 2017: Current Eye Research
https://www.readbyqxmd.com/read/29109898/limbal-stem-cell-deficiency-and-treatment-with-stem-cell-transplantation
#15
REVIEW
Özlem Barut Selver, Ayşe Yağcı, Sait Eğrilmez, Mehmet Gürdal, Melis Palamar, Türker Çavuşoğlu, Utku Ateş, Ali Veral, Çağrı Güven, Jose Mario Wolosin
The cornea is the outermost tissue of the eye and it must be transparent for the maintenance of good visual function. The superficial epithelium of the cornea, which is renewed continuously by corneal stem cells, plays a critical role in the permanence of this transparency. These stem cells are localized at the cornea-conjunctival transition zone, referred to as the limbus. When this zone is affected/destroyed, limbal stem cell deficiency ensues. Loss of limbal stem cell function allows colonization of the corneal surface by conjunctival epithelium...
October 2017: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/29107055/native-and-synthetic-scaffolds-for-limbal-epithelial-stem-cell-transplantation
#16
REVIEW
Kim N Nguyen, Samantha Bobba, Alexander Richardson, Mijeong Park, Stephanie L Watson, Denis Wakefield, Nick Di Girolamo
Limbal stem cell deficiency (LSCD) is a complex blinding disease of the cornea, which cannot be treated with conventional corneal transplants. Instead, a stem cell (SC) graft is required to replenish the limbal epithelial stem cell (LESC) reservoir, which is ultimately responsible for regenerating the corneal epithelium. Current therapies utilize limbal tissue biopsies that harbor LESCs as well as tissue culture expanded cells. Typically, this tissue is placed on a scaffold that supports the formation of corneal epithelial cell sheets, which are then transferred to diseased eyes...
November 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29106643/zika-virus-replicates-in-proliferating-cells-in-explants-from-first-trimester-human-placentas-potential-sites-for-dissemination-of-infection
#17
Takako Tabata, Matthew Petitt, Henry Puerta-Guardo, Daniela Michlmayr, Eva Harris, Lenore Pereira
Background: Maternal Zika virus (ZIKV) infection with prolonged viremia leads to fetal infection and congenital Zika syndrome. We reported that ZIKV infects primary cells from human placentas and fetal membranes. Here, we studied viral replication in numerous explants of anchoring villi and basal decidua from first-trimester human placentas and mid-gestation amniotic epithelial cells (AmEpCs). Methods: Explants and AmEpCs were infected with American and African ZIKV strains at low multiplicities, and ZIKV proteins were visualized by immunofluorescence...
November 2, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29106045/hyaluronic-acid-in-the-tail-and-limb-of-amphibians-and-lizards-recreates-permissive-embryonic-conditions-for-regeneration-due-to-its-hygroscopic-and-immunosuppressive-properties
#18
REVIEW
Lorenzo Alibardi
The present review focuses on the role of hyaluronate (hyaluronic acid; HA) during limb and tail regeneration in amphibians and lizards mainly in relation to cells of the immune system. This non-sulfated glycosaminoglycan (GAG) increases in early stages of wound healing and blastema formation, like during limb or tail embryogenesis, when the immune system is still immature. The formation of a regenerating blastema occurs by the accumulation of mesenchymal cells displaying embryonic-like antigens and HA. This GAG adsorbs large amount of water and generates a soft tissue over 80% hydrated where mesenchymal and epithelial cells can move and interact, an obligatory passage for organ regeneration...
November 6, 2017: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/29106037/mesenchymal-stem-cells-seeded-on-a-human-amniotic-membrane-improve-liver-regeneration-and-mouse-survival-after-extended-hepatectomy
#19
Aure Despeyroux, Cédric Duret, Claire Gondeau, Esther Perez-Gracia, Lisa Chuttoo, Hugues de Boussac, Philippe Briolotti, Claire Bony, Danièle Noël, Christian Jorgensen, Dominique Larrey, Martine Daujat-Chavanieu, Astrid Herrero
Liver failure remains the leading cause of postoperative mortality after hepatectomy. The present study investigated the effect of treatment with allogenic mesenchymal stem cells (MSCs) on survival and liver regeneration 48h and 7 days after 80% hepatectomy in C57Bl/6 mice. To optimize their biodistribution, MSCs were grown on acellular human amniotic membranes (HAM) and applied as a patch on the remnant liver. This approach was compared with MSC infusion and HAM patch alone. Hepatectomized mice without any treatment were used as control group...
November 6, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29105544/human-acellular-amniotic-membrane-a-potential-osteoinductive-biomaterial-for-bone-regeneration
#20
Kai Tang, Jiayi Wu, Zekang Xiong, Yanhui Ji, Tingfang Sun, Xiaodong Guo
Human acellular amniotic membrane is an acellular, naturally extracellular matrix material with various bioactive factors, which applied in tissue engineering in clinic. Several studies have applied human acellular amniotic membrane in skin and ocular surface tissue engineering to enhance tissue regeneration. However, the application of human acellular amniotic membrane in bone tissue engineering was rarely investigated. The aim of the current study was to investigate the osteoinductivity, angiogenesis and the early molecular changes of human acellular amniotic membrane to bone regeneration...
January 1, 2017: Journal of Biomaterials Applications
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