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ST101 Klebsiella

Lotfi Loucif, Ahmed Kassah Laouar, Mahdia Saidi, Amina Messala, Widad Chelaghma, Jean-Marc Rolain
Seven non-redundant ertapenem-resistant Klebsiella pneumoniae isolates were collected from May 2014 to January 19, 2015 in the nephrology and hematology units of Batna University Hospital, Algeria. All strains coproduced the blaOXA-48, blaCTX-M-15, blaSHV-1 and blaTEM-1D genes. Six of these isolates belonged to the pandemic clone ST101. The blaOXA-48 gene was located on a conjugative IncL/M-type plasmid. This is the first outbreak of OXA-48-producing K. pneumoniae isolates involving an ST101 clone in Batna University Hospital, Algeria...
September 19, 2016: Antimicrobial Agents and Chemotherapy
M De Laveleye, T D Huang, P Bogaerts, C Berhin, C Bauraing, P Sacré, A Noel, Y Glupczynski
Carbapenemase-producing Enterobacteriaceae are increasingly reported worldwide. The aim of the study was to determine the incidence and molecular epidemiology of carbapenemase-producing (CP) Escherichia coli and Klebsiella pneumoniae (CP-E/K) in Belgium. Eleven hospital-based laboratories collected carbapenem non-susceptible (CNS) isolates of E. coli and K. pneumoniae detected in clinical specimens from January 2013 to December 2014. All CNS strains were tested for carbapenemase production and typed by multilocus sequence typing (MLST) for a 6-month period as part of the European Survey on Carbapenemase-Producing Enterobacteriaceae in Europe (EuSCAPE) structured survey...
September 17, 2016: European Journal of Clinical Microbiology & Infectious Diseases
Jesús Oteo, María Pérez-Vázquez, Verónica Bautista, Adriana Ortega, Pilar Zamarrón, David Saez, Sara Fernández-Romero, Noelia Lara, Raquel Ramiro, Belén Aracil, José Campos
OBJECTIVES: We analysed the microbiological traits and population structure of KPC-producing Enterobacteriaceae isolates collected in Spain between 2012 and 2014. We also performed a comparative WGS analysis of the three major KPC-producing Klebsiella pneumoniae clones detected. METHODS: Carbapenemase and ESBL genes were sequenced. The Institut Pasteur MLST scheme was used. WGS data were used to construct phylogenetic trees, to identify the determinants of resistance and to de novo assemble the genome of one representative isolate of each of the three major K...
August 15, 2016: Journal of Antimicrobial Chemotherapy
Carolina Venditti, Laura Villa, Alessandro Capone, Daniela Fortini, Silvia D'Arezzo, Carla Nisii, Eugenio Bordi, Vincenzo Puro, Mario Antonini, Alessandra Carattoli, Maria Adriana Cataldo, Nicola Petrosillo, Antonino Di Caro
We describe the interspecies transmission of the plasmid-mediated blaKPC-3 gene, which confers carbapenem resistance, between clinically relevant gram-negative bacteria in a single patient. A KPC-3 producing Enterobacter aerogenes was isolated from a hospitalized patient previously colonized and then infected by a Klebsiella pneumoniae ST101 carrying the blaKPC-3 gene. The strains showed identical plasmids. Since intense horizontal exchanges among bacteria can occur in the gut, clinicians should be aware that patients colonized by carbapenem-resistant K...
June 10, 2016: New Microbiologica
Martin Kaase, Sven Schimanski, Reinhold Schiller, Bettina Beyreiß, Alexander Thürmer, Jörg Steinmann, Volkhard A Kempf, Christina Hess, Ingo Sobottka, Ines Fenner, Stefan Ziesing, Irene Burckhardt, Lutz von Müller, Axel Hamprecht, Ina Tammer, Nina Wantia, Karsten Becker, Thomas Holzmann, Martina Furitsch, Gabriele Volmer, Sören G Gatermann
Aim of this study was to determine the incidence and molecular epidemiology of carbapenemase-producing Escherichia coli and Klebsiella pneumoniae in Germany. E. coli and K. pneumoniae isolates from clinical samples which were non-susceptible to carbapenems were collected in laboratories serving 20 hospitals throughout Germany from November 2013 to April 2014. The isolates were tested for the presence of carbapenemases by PCR and phenotypic methods and typed by multilocus sequence typing. Risk factors including a previous hospitalization abroad were analysed...
September 2016: International Journal of Medical Microbiology: IJMM
Leila Ben Said, Ahlem Jouini, Carla Andrea Alonso, Naouel Klibi, Raoudha Dziri, Abdellatif Boudabous, Karim Ben Slama, Carmen Torres
The presence of extended-spectrum beta-lactamase and plasmid-mediated AmpC beta-lactamase producing Enterobacteriaceae (ESBL-Eb and pAmpC-Eb, respectively) was analyzed in 57 wastewater and 57 surface-water samples in Tunisia. Twenty-four of the 57 wastewater samples (42.1%) and one of the 57 surface-water samples (1.7%, a river that received effluents of a wastewater-treatment-plant) contained ESBL-Eb or pAmpC-Eb; one ESBL/pAmpC-Eb per positive sample was further characterized. Beta-lactamase genes detected were as follows: blaCTX-M-1 (10 Escherichia coli),blaCTX-M-15 (eight E...
April 15, 2016: Science of the Total Environment
Mariateresa Del Franco, Laura Paone, Roberto Novati, Claudio G Giacomazzi, Maria Bagattini, Chiara Galotto, Pier Giorgio Montanera, Maria Triassi, Raffaele Zarrilli
BACKGROUND: The spread of carbapenem resistant Enterobacteriaceae (CRE) is an emerging clinical problem, of great relevance in Europe and worldwide. The aim of this study was the molecular epidemiology of CRE isolates in Valle d'Aosta region, Italy, and the mechanism of carbapenem resistance. RESULTS: Sixty consecutive CRE samples were isolated from 52 hospital inpatients and/or outpatients from November 2013 to August 2014. Genotyping of microbial isolates was done by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST), carbapenemases were identified by PCR and sequencing...
2015: BMC Microbiology
Szilvia Melegh, György Schneider, Marianna Horváth, Ferenc Jakab, Levente Emődy, Zoltán Tigyi
We investigated the molecular epidemiology of extended spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae isolates derived from the teaching hospitals of University of Pécs, Pécs, Hungary in the time period 2004-2008. Molecular typing, antimicrobial susceptibility testing, detection of common β-lactamase genes (bla(CTX-M), bla(TEM) and bla(SHV)) and virulence associated traits (hypermucoviscosity, magA, k2a, rmpA, siderophores, type 1 and 3 fimbria, biofilm formation, serum resistance) were performed for 102 isolates...
September 2015: Acta Microbiologica et Immunologica Hungarica
Shoeib Nematzadeh, Fereshteh Shahcheraghi, Aina Iversen, Christian G Giske
The dissemination of plasmid-mediated multidrug resistance in Enterobacteriaceae is a major public health concern. We investigated the prevalence of plasmid-mediated quinolone resistance (PMQR), 16S rRNA methylases, CTX-M, and acquired AmpC enzymes in ESBL-producing Klebsiella pneumoniae (n=40) from Tehran hospitals. Plasmid replicon typing, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) were carried out for typing. CTX-M group 1 (confirmed as bla(CTX-M-15) in selected isolates) was found in 35/40 isolates...
December 2015: Diagnostic Microbiology and Infectious Disease
Raoudha Grami, Wejdene Mansour, Anis Ben Haj Khalifa, Safia Dahmen, Pierre Chatre, Marisa Haenni, Mahjoub Aouni, Jean-Yves Madec
Molecular features of the first carbapenem-resistant Klebsiella pneumoniae isolates (KP1 and KP2) from the University Hospital Tahar Sfar, Tunisia, were investigated. Antimicrobial susceptibility testing, multilocus sequence typing, S1 nuclease pulsed-field gel electrophoresis, Southern blot, and polymerase chain reaction (PCR)-based replicon typing were performed. Extended-spectrum β-lactamases and carbapenemase genes were detected by PCR and sequencing. Both isolates were multidrug resistant. KP1 was of sequence type (ST) ST101 and exhibited blaCTX-M-15 and blaTEM-1 on an untypeable plasmid and blaOXA-48 on an IncL/M plasmid...
March 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Wejdene Mansour, Raoudha Grami, Anis Ben Haj Khalifa, Safia Dahmen, Pierre Châtre, Marisa Haenni, Mahjoub Aouni, Jean-Yves Madec
This study investigated the molecular features of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae from hospital- and community-acquired (HA/CA) infections in the region of Mahdia, Tunisia. Among 336 K. pneumoniae isolates recovered from both clinical contexts between July 2009 and December 2011, 49 and 15 were ESBL producers and originated from clinical and community sources, respectively. All isolates produced the CTX-M-15 enzyme. As shown by Southern blot on S1 nuclease treatment followed by pulsed-field gel electrophoresis (PFGE) gels, the blaCTX-M-15 gene was carried on IncFII (n=4), IncFIIk (n=25), IncL/M (n=4), IncK (n=1), or untypeable (n=15) plasmids in HA isolates...
November 2015: Diagnostic Microbiology and Infectious Disease
Meritxell Cubero, Guillermo Cuervo, M Ángeles Dominguez, Fe Tubau, Sara Martí, Elena Sevillano, Lucía Gallego, Josefina Ayats, Carmen Peña, Miquel Pujol, Josefina Liñares, Carmen Ardanuy
BACKGROUND: In this study we describe the clinical and molecular characteristics of an outbreak due to carbapenem-resistant Klebsiella pneumoniae (CR-KP) producing CTX-M-15 and OXA-48 carbapenemase. Isogenic strains, carbapenem-susceptible K. pneumoniae (CS-KP) producing CTX-M-15, were also involved in the outbreak. RESULTS: From October 2010 to December 2012 a total of 62 CR-KP and 23 CS-KP were isolated from clinical samples of 42 patients (22 had resistant isolates, 14 had susceptible isolates, and 6 had both CR and CS isolates)...
2015: BMC Microbiology
Rumyana Markovska, Temenuga Stoeva, Ines Schneider, Lyudmila Boyanova, Valentina Popova, Daniela Dacheva, Radka Kaneva, Adolf Bauernfeind, Vanyo Mitev, Ivan Mitov
A total of 36 consecutive clinical and two fecal-screening carbapenem-resistant Klebsiella pneumoniae isolates from two Bulgarian university hospitals (Varna and Pleven) were investigated. Susceptibility testing, conjugation experiments, and plasmid replicon typing were carried out. Beta-lactamases were characterized by isoelectric focusing, PCR, and sequencing. Clonal relatedness was investigated by RAPD and multilocus sequence typing (MLST). Most of the isolates demonstrated multidrug resistance profile. Amikacin and tigecycline retained good activity with susceptibility rates of 95 and 87%, respectively...
October 2015: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
J Hrabák, V Študentová, V Jakubů, V Adámková, L Dvořáková, M Balejova, T Bergerová, E Chmelařová, P Ježek, P Kabelíková, M Kolář, P Paterová, R Tejkalová, C Papagiannitsis, H Žemličková
OBJECTIVE: One of the most important threats of current medicine is the spread of multiresistant Gram-negative bacteria. We report here data from a six-month prevalence study on carbapenemase-producing K. pneumoniae and E. coli performed in Czech hospitals participating on European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE). METHODS: Ten hospitals covering all regions of the Czech Republic were selected. During the study period (1st November 2013 to 30th April 2014), first ten carbapenem non-susceptible isolates of K...
June 2015: Epidemiologie, Mikrobiologie, Imunologie
Stephen E Mshana, Moritz Fritzenwanker, Linda Falgenhauer, Eugen Domann, Torsten Hain, Trinad Chakraborty, Can Imirzalioglu
BACKGROUND: Multi-drug resistant Klebsiella pneumoniae strains are a common cause of health care associated infections worldwide. Clonal spread of Klebsiella pneumoniae isolates carrying plasmid mediated CTX-M-15 have been commonly reported. Limited data is available regarding dissemination of chromosomally encoded CTX-M-15 in Klebsiella pneumoniae worldwide. RESULTS: We examined 23 non-repetitive ESBL-producing Klebsiella pneumoniae strains isolated from clinical specimens over a period of 4 months in a German University Hospital...
2015: BMC Microbiology
Karama Charfi, Wejdène Mansour, Anis Ben Haj Khalifa, Maha Mastouri, Mahjoub Aouni, Hedi Mammeri
A retrospective epidemiological survey was carried out to determine the prevalence of carbapenemase producers among enterobacterial clinical isolates recovered in the center of maternity and neonatology of Monastir (Tunisia). PCR screening identified 1 OXA-48 and 2 OXA-204 producers, which coexpressed the CTX-M-15 or the CMY-4 β-lactamases. PCR mapping showed that the bla(OXA-48) gene was carried by a Tn1999.2 transposon, whereas the bla(OXA-204) gene was part of the Tn2016 transposon-like structure. The OXA-48- or OXA-204-producing Klebsiella pneumoniae clinical isolates and the OXA-204-expressing Escherichia coli clinical isolate belonged to the widespread sequence types ST11, ST101, and ST617, respectively...
August 2015: Diagnostic Microbiology and Infectious Disease
Pia S Pannaraj, Jennifer Dien Bard, Chiara Cerini, Scott J Weissman
BACKGROUND: Infections caused by carbapenem-resistant Enterobacteriaceae (CRE) are on the rise worldwide but are not well described in pediatric populations. This study characterizes the clinical, phenotypic and genotypic characteristics of CRE infections at a free-standing US children's hospital. METHODS: CRE were defined as any clinical Enterobacteriaceae isolate non-susceptible to either imipenem or meropenem and resistant to ceftriaxone, cefotaxime and ceftazidime determined by routine antimicrobial susceptibility testing...
January 2015: Pediatric Infectious Disease Journal
E Liapis, A Pantel, J Robert, M-H Nicolas-Chanoine, L Cavalié, N van der Mee-Marquet, C de Champs, N Aissa, C Eloy, V Blanc, C Guyeux, D Hocquet, J-P Lavigne, X Bertrand
We characterized 53 OXA-48-producing Klebsiella pneumoniae (OXA-48-Kp) isolated between 2011 and 2013 in 21 French hospitals. All the isolates were genotyped using MLST and PFGE and the population structure of the species was determined by a nucleotide-based analysis of the entire K. pneumoniae MLST database. Most of the OXA-48-Kp isolates also produced CTX-M-15 and remained susceptible to imipenem and meropenem. The isolates were distributed into 20 STs, of which five were dominant (ST15, ST101, ST147, ST395 and ST405)...
December 2014: Clinical Microbiology and Infection
Aurélie Jayol, Laurent Poirel, Adrian Brink, Maria-Virginia Villegas, Mesut Yilmaz, Patrice Nordmann
A series of colistin-resistant Klebsiella pneumoniae isolates recovered from different countries was investigated in order to evaluate the involvement of the PmrA/PmrB two-component system in this resistance. Six isolates possessed a mutated PmrB protein, which is encoded by the pmrB gene, part of the pmrCAB operon involved in lipopolysaccharide modification. The same amino acid substitution (Thr157Pro) in PmrB was identified in the six isolates. The six isolates belonged to four distinct clonal groups, recovered in South Africa (sequence type 14 [ST14]), Turkey (ST101), and Colombia (ST258 and ST15)...
August 2014: Antimicrobial Agents and Chemotherapy
Irene Pena, Juan J Picazo, Carmen Rodríguez-Avial, Iciar Rodríguez-Avial
Here we describe the carbapenemase genes, genetic relatedness and antimicrobial susceptibility data of 123 carbapenemase-producing Enterobacteriaceae (CPE) clinical isolates recovered from 2010 to 2012, comprising Klebsiella pneumoniae (n = 79), Klebsiella oxytoca (n = 13), Serratia marcescens (n = 14), Enterobacter cloacae (n = 12), Enterobacter asburiae (n = 4) and Enterobacter aerogenes (n = 1). VIM-1 was the most common carbapenemase (n = 101) followed by KPC-2 (n = 19), OXA-48 (n = 2) and IMP-22 (n = 1)...
May 2014: International Journal of Antimicrobial Agents
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