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ST101 Klebsiella

Torunn Pedersen, John Osei Sekyere, Usha Govinden, Krishnee Moodley, Audun Sivertsen, Ørjan Samuelsen, Sabiha Yusuf Essack, Arnfinn Sundsfjord
Whole-genome sequence analyses revealed the presence of bla NDM-1 ( n =31), bla GES-5 ( n =8), bla OXA-232 ( n =1), or bla NDM-5 ( n =1) in extensively drug-resistant and pandrug-resistant Enterobacteriaceae isolated from in-patients in 10 private hospitals (2012-2013) in Durban, South Africa. Two novel NDM-1 encoding plasmids from Klebsiella pneumoniae were closed by PacBio sequencing. In p19-10_01 (IncFIB(K), 223.434 bp) bla NDM-1 was part of a Tn 1548 -like structure (16.276 bp) delineated by IS 26 The multireplicon plasmid p18-43_01 (IncR_1/IncFIB(pB171)/IncFII(Yp); 212...
March 5, 2018: Antimicrobial Agents and Chemotherapy
Leonardo Neves Andrade, Ângela Novais, Lenita Maria Marcato Stegani, Joseane Cristina Ferreira, Carla Rodrigues, Ana Lucia Costa Darini, Luisa Peixe
We performed a single-month snapshot study of the population diversity of multidrug resistant (MDR) Klebsiella pneumoniae isolates producing carbapenemases and/or extended-spectrum β-lactamases from four major hospitals in Brazil. Isolates produced diverse ESBL (CTX-M-2, -8, -15, SHV-2), KPC-2 or both (CTX-M-2 and KPC-2), linked to specific genetic backgrounds and plasmids from a few families (IncR, IncFIIk, IncL/M) that were shared among clonal lineages within and between hospitals. A high clonal diversity was identified, among isolates from the same ST (ST11, ST15, ST101 or ST340)...
January 12, 2018: Diagnostic Microbiology and Infectious Disease
Fusun Can, Sirin Menekse, Pelin Ispir, Nazli Atac, Ozgur Albayrak, Tuana Demir, Doruk Can Karaaslan, Salih Nafiz Karahan, Mahir Kapmaz, Ozlem Kurt Azap, Funda Timurkaynak, Serap Simsek Yavuz, Seniha Basaran, Fugen Yoruk, Alpay Azap, Safiye Koculu, Nur Benzonana, Nathan A Lack, Mehmet Gönen, Onder Ergonul
Objectives: We describe the molecular characteristics of colistin resistance and its impact on patient mortality. Methods: A prospective cohort study was performed in seven different Turkish hospitals. The genotype of each isolate was determined by MLST and repetitive extragenic palindromic PCR (rep-PCR). Alterations in mgrB were detected by sequencing. Upregulation of pmrCAB, phoQ and pmrK was quantified by RT-PCR. mcr-1 and the genes encoding OXA-48, NDM-1 and KPC were amplified by PCR...
February 3, 2018: Journal of Antimicrobial Chemotherapy
V Nordberg, K Jonsson, C G Giske, A Iversen, O Aspevall, B Jonsson, A Camporeale, M Norman, L Navér
OBJECTIVE: To analyse Klebsiella pneumoniae (KP) isolates from an outbreak of Extended-Spectrum β-Lactamase (ESBL)-producing KP and Escherichia coli (ESBL-EC) among infants admitted to neonatal intensive care units (NICUs) and to determine the duration of the intestinal colonization. METHODS: A prospective cohort study of intestinal ESBL-KP/ ESBL-EC colonized neonates after a five-month outbreak in two NICUs. Whole genome sequencing (WGS), multilocus sequence typing (MLST), core genome MLST, pulsed-field electrophoresis (PFGE) and PCR for blaCTX-M were performed on the first isolates...
January 8, 2018: Clinical Microbiology and Infection
Farah Ben Tanfous, Wafa Achour, Anis Raddaoui, Assia Ben Hassen
OBJECTIVES: This study investigated the prevalence of extended-spectrum β-lactamase producing K. pneumoniae (ESBL-KP) strains in the National Bone Marrow Transplant Center of Tunis between 2002 and 2011, their associated antibiotics resistance patterns and their molecular features. METHODS: Antimicrobial susceptibility was determined by the disk diffusion method according to CA-SFM guidelines. All the strains were screened for beta-lactamase genes, plasmidic AmpC genes and integrons...
January 5, 2018: Journal of Global Antimicrobial Resistance
R Izdebski, A Baraniak, D Zabicka, M Machulska, P Urbanowicz, J Fiett, E Literacka, K Bojarska, A Kozinska, B Zieniuk, W Hryniewicz, M Gniadkowski
Objectives: To analyse OXA-48 (OXA-48/181)-type carbapenemase-producing Enterobacteriaceae reported in Poland from 2013 until January 2017. Methods: Bacterial isolates were typed by PFGE and MLST. Genes coding for OXA-48/181 types and other β-lactamases were amplified and sequenced. Mobile elements with blaOXA-48/181-like genes were PCR mapped. blaOXA-48/181-carrying plasmids were characterized by nuclease S1-hybridization profiling, transfer assays and PCR-based replicon typing, while the chromosomal location of the genes was confirmed by the I-CeuI analysis...
December 9, 2017: Journal of Antimicrobial Chemotherapy
Nadia Jaidane, Rémy A Bonnin, Wejdene Mansour, Delphine Girlich, Elodie Creton, Garance Cotellon, Cherifa Chaouch, Noureddine Boujaafar, Olfa Bouallegue, Thierry Naas
The emergence of colistin-resistant Klebsiella pneumoniae (CoR Kp ) is a public health concern, since this antibiotic has become the last line of treatment for infections caused by multidrug-resistant (MDR) Gram negatives. In this study, we have investigated the molecular basis of colistin resistance in 13 MDR K. pneumoniae strains isolated from 12 patients in a teaching hospital in Sousse, Tunisia. Whole-genome sequencing (WGS) was used to decipher the molecular mechanism of colistin resistance and to identify the resistome of these CoR Kp isolates...
February 2018: Antimicrobial Agents and Chemotherapy
Guilherme Bartolomeu Gonçalves, João Pedro Rueda Furlan, Eliana Carolina Vespero, Marsileni Pelisson, Eliana Guedes Stehling, André Pitondo-Silva
Klebsiella pneumoniae is among the most important pathogens found in hospitals. The emergence of multiple antibiotic resistant K. pneumoniae associated with its virulence factors is a worldwide concern and its early identification is crucial, especially for controlling the spread of emerging clones. This article reports a high prevalence of multiresistant K. pneumoniae in a university hospital in southern Brazil, harboring several virulence and β-lactamase encoding genes, including pandrug-resistant high-risk international clones belonging to the clonal group 258 (ST11, ST15, ST101, ST258, ST340 and ST874)...
December 2017: Infection, Genetics and Evolution
Wejdène Mansour, Marisa Haenni, Estelle Saras, Raoudha Grami, Yosra Mani, Anis Ben Haj Khalifa, Souhail El Atrouss, Mohamed Kheder, Mohamed Fekih Hassen, Noureddine Boujâafar, Olfa Bouallegue, Jean-Yves Madec
OBJECTIVES: Mechanisms of colistin and carbapenem resistance among a collection of Klebsiella pneumoniae isolates recovered in a university hospital in Tunisia were studied. METHODS: In vitro antimicrobial susceptibility testing, S1 nuclease pulsed-field gel electrophoresis (S1-PFGE), Southern blotting and PCR-based replicon typing (PBRT) were performed. Extended-spectrum β-lactamases (ESBLs), carbapenemases, AmpC-type enzymes and mgrB genes were detected by PCR and sequencing...
September 2017: Journal of Global Antimicrobial Resistance
Avemaria Obasi, SimonCyril Nwachukwu, Esther Ugoji, Christian Kohler, André Göhler, Veronica Balau, Yvonne Pfeifer, Ivo Steinmetz
Emergence and spread of Klebsiella pneumoniae isolates producing extended-spectrum β-lactamases (ESBLs) present a major threat to public health. In this study, we characterized β-lactam-resistant K. pneumoniae isolates from six wastewater samples obtained from two pharmaceutical industries located in Lagos and Ogun States, Nigeria. Bacteria were isolated by using MacConkey agar; species identification and antibacterial susceptibility testing were performed by Vitek 2. Etest was used for ESBL phenotype confirmation...
December 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Katarina Novović, Anika Trudić, Snežana Brkić, Zorica Vasiljević, Milan Kojić, Deana Medić, Ivana Ćirković, Branko Jovčić
Twenty-seven colistin-resistant, carbapenemase-producing Klebsiella pneumoniae isolates were identified from hospitals in Serbia. All isolates were blaCTX-M-15 positive; ST101, ST888, ST437, ST336, and ST307 were blaOXA-48 positive; and ST340 was blaNDM-1 positive. ST307 had an insertion, and ST336 had a premature stop codon in the mgrB gene. Amino acid substitutions were detected in PmrAB of isolates ST101, ST888, ST336, and ST307. The mcr-1 and mcr-2 were not detected. An increase in phoP, phoQ, and pmrK gene transcription was detected for all sequence types...
May 2017: Antimicrobial Agents and Chemotherapy
Jing Yu, Yue Wang, Zhongju Chen, Xuhui Zhu, Lei Tian, Li Li, Ziyong Sun
OBJECTIVES: The aim of this study was to characterise carbapenem-resistant Klebsiella pneumoniae isolates recovered from neonatal clinical specimens over a 4-month period. METHODS: Seven carbapenem-resistant K. pneumoniae isolates were analysed. Antibiotic susceptibilities of the isolates were determined by the agar dilution method, and the drug resistance genes were evaluated by PCR. Clonal relatedness of the isolates was assessed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST)...
January 18, 2017: Journal of Global Antimicrobial Resistance
Ye-Mun Low, Polly Soo-Xi Yap, Kartini Abdul Jabar, Sasheela Ponnampalavanar, Rina Karunakaran, Rukumani Velayuthan, Chun-Wie Chong, Sazaly Abu Bakar, Mohd Yasim Md Yusof, Cindy Shuan-Ju Teh
BACKGROUND: Carbapenem resistant Enterobacteriaceae is a growing concern worldwide including Malaysia. The emergence of this pathogen is worrying because carbapenem is one of the 'last-line' antibiotics. The main objective of this study was to determine the prevalence of genetic mechanisms and clinical risk factors of carbapenem resistant Klebsiella pneumoniae (K. pneumoniae) in Malaysia. METHODS: In this study, seventeen carbapenem resistant K. pneumoniae strains isolated from a tertiary teaching hospital in 2013 were studied...
2017: Antimicrobial Resistance and Infection Control
Elaa Maamar, Sana Ferjani, Ali Jendoubi, Samia Hammami, Zaineb Hamzaoui, Laure Mayonnove-Coulange, Mabrouka Saidani, Aouatef Kammoun, Amel Rehaiem, Salma Ghedira, Mohamed Houissa, Ilhem Boutiba-Ben Boubaker, Amine Slim, Veronique Dubois
Healthcare-associated infections due to cefotaxime-resistant (CTX-R) Enterobacteriaceae have become a major public health threat, especially in intensive care units (ICUs). Often acquired nosocomially, CTX-R Enterobacteriaceae can be introduced initially by patients at admission. This study aimed to determine the prevalence and genetic characteristics of CTX-R Enterobacteriaceae-intestinal carriage in ICU patients, to evaluate the rate of acquisition of these organisms during hospitalization, and to explore some of the associated risk factors for both carriage and acquisition...
2016: Frontiers in Microbiology
Anna Skalova, Katerina Chudejova, Veronika Rotova, Matej Medvecky, Vendula Studentova, Eva Chudackova, Pavel Lavicka, Tamara Bergerova, Vladislav Jakubu, Helena Zemlickova, Costas C Papagiannitsis, Jaroslav Hrabak
The aim of this study was to characterize the first cases and outbreaks of OXA-48-like-producing Enterobacteriaceae recovered from hospital settings in the Czech Republic. From 2013 to 2015, 22 Klebsiella pneumoniae isolates, 3 Escherichia coli isolates, and 1 Enterobacter cloacae isolate producing OXA-48-like carbapenemases were isolated from 20 patients. Four of the patients were colonized or infected by two or three different OXA-48-like producers. The K. pneumoniae isolates were classified into nine sequence types (STs), with ST101 being predominant (n = 8)...
February 2017: Antimicrobial Agents and Chemotherapy
Lotfi Loucif, Ahmed Kassah-Laouar, Mahdia Saidi, Amina Messala, Widad Chelaghma, Jean-Marc Rolain
Seven nonredundant ertapenem-resistant Klebsiella pneumoniae isolates were collected between May 2014 and 19 January 2015 in the nephrology and hematology units of Batna University Hospital in Algeria. All strains coproduced the blaOXA-48, blaCTX-M-15, blaSHV-1, and blaTEM-1D genes. Six of these isolates belonged to the pandemic clone sequence type 101 (ST101). The blaOXA-48 gene was located on a conjugative IncL/M-type plasmid. This is the first known outbreak of OXA-48-producing K. pneumoniae isolates involving an ST101 clone in Batna University Hospital...
December 2016: Antimicrobial Agents and Chemotherapy
M De Laveleye, T D Huang, P Bogaerts, C Berhin, C Bauraing, P Sacré, A Noel, Y Glupczynski
Carbapenemase-producing Enterobacteriaceae are increasingly reported worldwide. The aim of the study was to determine the incidence and molecular epidemiology of carbapenemase-producing (CP) Escherichia coli and Klebsiella pneumoniae (CP-E/K) in Belgium. Eleven hospital-based laboratories collected carbapenem non-susceptible (CNS) isolates of E. coli and K. pneumoniae detected in clinical specimens from January 2013 to December 2014. All CNS strains were tested for carbapenemase production and typed by multilocus sequence typing (MLST) for a 6-month period as part of the European Survey on Carbapenemase-Producing Enterobacteriaceae in Europe (EuSCAPE) structured survey...
January 2017: European Journal of Clinical Microbiology & Infectious Diseases
Jesús Oteo, María Pérez-Vázquez, Verónica Bautista, Adriana Ortega, Pilar Zamarrón, David Saez, Sara Fernández-Romero, Noelia Lara, Raquel Ramiro, Belén Aracil, José Campos
OBJECTIVES: We analysed the microbiological traits and population structure of KPC-producing Enterobacteriaceae isolates collected in Spain between 2012 and 2014. We also performed a comparative WGS analysis of the three major KPC-producing Klebsiella pneumoniae clones detected. METHODS: Carbapenemase and ESBL genes were sequenced. The Institut Pasteur MLST scheme was used. WGS data were used to construct phylogenetic trees, to identify the determinants of resistance and to de novo assemble the genome of one representative isolate of each of the three major K...
December 2016: Journal of Antimicrobial Chemotherapy
Carolina Venditti, Laura Villa, Alessandro Capone, Daniela Fortini, Silvia D'Arezzo, Carla Nisii, Eugenio Bordi, Vincenzo Puro, Mario Antonini, Alessandra Carattoli, Maria Adriana Cataldo, Nicola Petrosillo, Antonino Di Caro
We describe the interspecies transmission of the plasmid-mediated blaKPC-3 gene, which confers carbapenem resistance, between clinically relevant gram-negative bacteria in a single patient. A KPC-3 producing Enterobacter aerogenes was isolated from a hospitalized patient previously colonized and then infected by a Klebsiella pneumoniae ST101 carrying the blaKPC-3 gene. The strains showed identical plasmids. Since intense horizontal exchanges among bacteria can occur in the gut, clinicians should be aware that patients colonized by carbapenem-resistant K...
October 2016: New Microbiologica
Martin Kaase, Sven Schimanski, Reinhold Schiller, Bettina Beyreiß, Alexander Thürmer, Jörg Steinmann, Volkhard A Kempf, Christina Hess, Ingo Sobottka, Ines Fenner, Stefan Ziesing, Irene Burckhardt, Lutz von Müller, Axel Hamprecht, Ina Tammer, Nina Wantia, Karsten Becker, Thomas Holzmann, Martina Furitsch, Gabriele Volmer, Sören G Gatermann
Aim of this study was to determine the incidence and molecular epidemiology of carbapenemase-producing Escherichia coli and Klebsiella pneumoniae in Germany. E. coli and K. pneumoniae isolates from clinical samples which were non-susceptible to carbapenems were collected in laboratories serving 20 hospitals throughout Germany from November 2013 to April 2014. The isolates were tested for the presence of carbapenemases by PCR and phenotypic methods and typed by multilocus sequence typing. Risk factors including a previous hospitalization abroad were analysed...
September 2016: International Journal of Medical Microbiology: IJMM
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