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https://www.readbyqxmd.com/read/29681865/quercetin-increases-muc2-and-muc5ac-gene-expression-and-secretion-in-intestinal-goblet-cell-like-ls174t-via-plc-pkc%C3%AE-erk1-2-pathway
#1
Simona Damiano, Anna Sasso, Bruna De Felice, Ilaria Di Gregorio, Giuliana La Rosa, Gelsi A Lupoli, Anna Belfiore, Paolo Mondola, Mariarosaria Santillo
The main dietary flavonoid quercetin, is known to preserve the integrity of gastrointestinal barrier and to have anti-inflammatory, anti-cancer, anti-fibrotic, and other beneficial properties. Many of the biological effects of quercetin appear to be associated to the modulation of cell signaling pathways, rather than to its antioxidant activity. In spite of the large number of data available on the molecular and cellular mechanisms by which quercetin exerts its biological effects, including protection of intestinal barrier function, there is a lack of data about the role of this substance on the expression and/or the secretion of mucins released by intestinal goblet cells...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29675856/adreno-muscarinic-synergy-in-the-male-human-urinary-outflow-tract
#2
Sebastian Walther, Frank Strittmatter, Martin Hennenberg, Christian Gratzke, Christian G Stief, Alexander Roosen
AIM: To examine putative interaction between adrenergic and muscarinic contractile activation in the human urinary outflow tract. METHODS: Tissue from the trigone and prostatic urethra was obtained from 12 cystectomy and 16 prostatectomy specimen. Contractions were elicited by exposure to exogenous agonists before and after inhibition of Rho kinase and protein kinase c (PKC). Immunofluorescence and Western-blot studies were performed using antibodies to muscarinic M3-receptors (M3-R) and alpha1A-adrenoreceptors (alpha1A-AR)...
April 20, 2018: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/29674665/the-proximal-tubular-%C3%AE-7-nicotinic-acetylcholine-receptor-attenuates-ischemic-acute-kidney-injury-through-akt-pkc-signaling-mediated-ho-1-induction
#3
Hwajin Kim, So Ra Kim, Jihyun Je, Kyuho Jeong, Sooji Kim, Hye Jung Kim, Ki Churl Chang, Sang Won Park
Activation of the α7 nicotinic acetylcholine receptor (α7nAChR) has been shown to attenuate excessive inflammation by inhibiting proinflammatory cytokines during ischemia-reperfusion (IR) injury; however, the underlying kidney-specific molecular mechanisms remain unclear. The protective action of α7nAChR against renal IR injury was investigated using a selective α7nAChR agonist and antagonist. α7nAChR activation reduced plasma creatinine levels and tubular cell damage, whereas α7nAChR inhibition aggravated the IR-induced phenotype...
April 20, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29672451/spinal-protein-kinase-c-extracellular-signal-regulated-kinase-signal-pathway-mediates-hyperalgesia-priming
#4
Wei-Hsin Chen, Ya-Ting Chang, Yong-Cyuan Chen, Sin-Jhong Cheng, Chien-Chang Chen
Chronic pain can be initiated by one or more acute stimulations to sensitize neurons into the primed state. In the primed state, the basal nociceptive thresholds of the animal are normal, but, in response to another hyperalgesic stimulus, the animal develops enhanced and prolonged hyperalgesia. The exact mechanism of how primed state is formed is not completely understood. Here, we showed that spinal protein kinase C (PKC)/extracellular signal-regulated kinase (ERK) signal pathway is required for neuronal plasticity change, hyperalgesic priming formation, and the development of chronic hyperalgesia using acid-induced muscle pain model in mice...
May 2018: Pain
https://www.readbyqxmd.com/read/29672153/p21-activated-kinase4-in-pancreatic-acini-is-activated-by-gi-hormones-growth-factors-by-novel-signaling-and-is-needed-to-stimulate-secretory-growth-cascades
#5
Irene Ramos-Alvarez, Robert T Jensen
p21-activated kinases(PAKs) are highly conserved serine/threonine protein kinases, which are divided into two groups:Group-I(PAKs1-3) and Group-II(PAKs4-6). In various tissues, Group-II PAKs play important roles in cytoskeletal dynamics, cell growth as well as neoplastic development/progression. However, little is known about Group-II PAKs role in a number of physiological events including their ability to be activated by gastrointestinal(GI) hormones/neurotransmitters/growth-factors(GFs). We used rat pancreatic acini to explore the ability of GI-hormones/neurotransmitters/GFs to activate Group-II-PAKs and the signaling cascades involved...
April 19, 2018: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/29671254/co-enzyme-q10-upregulates-hsp70-and-protects-chicken-primary-myocardial-cells-under-in-vitro-heat-stress-via-pkc-mapk
#6
Jiao Xu, Shu Tang, Bin Yin, Jiarui Sun, Endong Bao
In this report, we investigate the protective mechanism of co-enzyme Q10 on chicken primary myocardial cells during heat stress. Morphological observations indicate that addition of co-enzyme Q10 protects myocardial cells from heat stress, reduces the damage of mitochondria and nucleus, and decreases the mean number of vacuolated mitochondria. We have previously shown that co-enzyme Q10 can protect myocardial cells by upregulating the expression of Hsp70. Therefore, signaling pathways involved in this process were explored...
April 18, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29670278/blt1-in-dendritic-cells-promotes-th1-th17-differentiation-and-its-deficiency-ameliorates-tnbs-induced-colitis
#7
Jinfeng Zhou, Weiming Lai, Wanjie Yang, Juping Pan, Hu Shen, Yingying Cai, Cuixia Yang, Ningjia Ma, Yue Zhang, Ru Zhang, Xin Xie, Zhongjun Dong, Yuan Gao, Changsheng Du
Leukotriene B4 (LTB4) synthesis is enhanced in the colonic mucosa in patients with inflammatory bowel disease (IBD). BLT1, a high-affinity receptor for LTB4, exhibits no effect on the progression of dextran sodium sulfate (DSS)-induced colitis, which mostly relies on innate immunity. Here, we reported that BLT1 regulates trinitrobenzene sulfonic acid (TNBS)-induced colitis, which reflects CD4+ T-cell-dependent adaptive immune mechanisms of IBD. We found that BLT1 signaling enhanced the progression of colitis through controlling the production of proinflammatory cytokines by dendritic cells (DCs) and modulating the differentiation of Th1 and Th17...
April 18, 2018: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/29669290/non-canonical-opioid-signaling-inhibits-itch-transmission-in-the-spinal-cord-of-mice
#8
Admire Munanairi, Xian-Yu Liu, Devin M Barry, Qianyi Yang, Jun-Bin Yin, Hua Jin, Hui Li, Qing-Tao Meng, Jia-Hang Peng, Zhen-Yu Wu, Jun Yin, Xuan-Yi Zhou, Li Wan, Ping Mo, Seungil Kim, Fu-Quan Huo, Joseph Jeffry, Yun-Qing Li, Rita Bardoni, Michael R Bruchas, Zhou-Feng Chen
Chronic itch or pruritus is a debilitating disorder that is refractory to conventional anti-histamine treatment. Kappa opioid receptor (KOR) agonists have been used to treat chronic itch, but the underlying mechanism remains elusive. Here, we find that KOR and gastrin-releasing peptide receptor (GRPR) overlap in the spinal cord, and KOR activation attenuated GRPR-mediated histamine-independent acute and chronic itch in mice. Notably, canonical KOR-mediated Gαi signaling is not required for desensitizing GRPR function...
April 17, 2018: Cell Reports
https://www.readbyqxmd.com/read/29661832/kinase-activity-in-the-olfactory-bulb-is-required-for-odor-memory-consolidation
#9
Michelle T Tong, Tae-Young P Kim, Thomas A Cleland
Long-term fear memory formation in the hippocampus and neocortex depends upon brain-derived neurotrophic factor (BDNF) signaling after acquisition. Incremental, appetitive odor discrimination learning is thought to depend substantially on the differentiation of adult-born neurons within the olfactory bulb (OB)-a process that is closely associated with BDNF signaling. We sought to elucidate the role of neurotrophin signaling within the OB on odor memory consolidation. Male mice were trained on odor-reward associative discriminations after bilateral infusion of the kinase inhibitor K252a, or vehicle control, into the OB...
May 2018: Learning & Memory
https://www.readbyqxmd.com/read/29660932/neuroprotective-effects-of-ferruginol-jatrophone-and-junicedric-acid-against-amyloid-%C3%AE-injury-in-hippocampal-neurons
#10
Juan M Zolezzi, Carolina B Lindsay, Felipe G Serrano, Roxana C Ureta, Cristina Theoduloz, Guillermo Schmeda-Hirschmann, Nibaldo C Inestrosa
Soluble amyloid-β (Aβ) oligomers have been recognized as early neurotoxic intermediates with a key role in the synaptic dysfunction observed in Alzheimer's disease (AD). Aβ oligomers block hippocampal long-term potentiation (LTP) and impair rodent spatial memory. Additionally, the presence of Aβ oligomers is associated with imbalanced intracellular calcium levels and apoptosis in neurons. In this context, we evaluated the effects of three diterpenes (ferruginol, jatrophone, and junicedric acid) that are found in medicinal plants and have several forms of biological activity...
April 10, 2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29660437/deoxycholylglycine-a-conjugated-secondary-bile-acid-reduces-vascular-tone-by-attenuating-ca-2-sensitivity-via-rho-kinase-pathway
#11
Ravirajsinh N Jadeja, Menaka C Thounaojam, Manuela Bartoli, Sandeep Khurana
Patients with cirrhosis have reduced systemic vascular resistance and elevated circulating bile acids (BAs). Previously, we showed that secondary conjugated BAs impair vascular tone by reducing vascular smooth muscle cell (VSMC) Ca2+ influx. In this study, we investigated the effect of deoxycholylglycine (DCG), on Ca2+ sensitivity in reducing vascular tone. First, we evaluated the effects of DCG on U46619- and phorbol-myristate-acetate (PMA)-induced vasoconstriction. DCG reduced U46619-induced vascular tone but failed to reduce PMA-induced vasoconstriction...
April 13, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29660338/different-molecular-mechanisms-of-htlv-1-and-hiv-ltr-activation-by-tpa
#12
Azhar Jabareen, Manal Salman, Aya Abu-Jaafar, Mahmoud Huleihel
HTLV-1 and HIV-1 are retroviruses involved in different human diseases. However, following infection, these viruses inter into a latent state. Tax and Tat are regarded as trans-activators of HTLV-1 and HIV-1 respectively. As it known, during the latent state the infected cells contain low Tax and Tat protein levels, so the activation of these viruses must be independent of these proteins. Here we focus on exploring the mechanism of activation of these viruses by 12-O-tetradecanoylphorbol-13-acetate (TPA), which is a potent activator of protein kinase C (PKC) and considered as a stress-inducing agent...
April 13, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29659148/hydrogen-sulphide-increases-pulmonary-veins-and-atrial-arrhythmogenesis-with-activation-of-protein-kinase-c
#13
Chao-Shun Chan, Yung-Kuo Lin, Yu-Hsun Kao, Yao-Chang Chen, Shih-Ann Chen, Yi-Jen Chen
Hydrogen sulphide (H2 S), one of the most common toxic air pollutants, is an important aetiology of atrial fibrillation (AF). Pulmonary veins (PVs) and left atrium (LA) are the most important AF trigger and substrate. We investigated whether H2 S may modulate the arrhythmogenesis of PVs and atria. Conventional microelectrodes and whole-cell patch clamp were performed in rabbit PV, sinoatrial node (SAN) or atrial cardiomyocytes before and after the perfusion of NaHS with or without chelerythrine (a selective PKC inhibitor), rottlerin (a specific PKC δ inhibitor) or KB-R7943 (a NCX inhibitor)...
April 16, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29659066/a-mass-spectrometry-based-predictive-strategy-reveals-adap1-is-phosphorylated-at-tyrosine-364
#14
Richard Reisdorph, BobbiJo Littrell-Miller, Roger Powell, Nichole Reisdorph
RATIONALE: The goal of this work was to identify phosphorylation sites within the amino acid sequence of human ADAP1. Using traditional mass spectrometry-based techniques we were unable to produce interpretable spectra demonstrating modification by phosphorylation. This prompted us to employ a strategy in which phosphorylated peptides were first predicted using peptide mapping followed by targeted MS/MS acquisition. METHODS: ADAP1 was immunoprecipitated from extracts of HEK293 cells stably-transfected with ADAP1 cDNA...
April 16, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29658601/silencing-of-casein-kinase-2-inhibits-pkc%C3%A2-induced-cell-invasion-by-targeting-mmp%C3%A2-9-in-mcf%C3%A2-7-cells
#15
Jeong-Mi Kim, Eun-Mi Noh, Hyun-Kyung Song, Yong-Ouk You, Sung Hoo Jung, Jong-Suk Kim, Kang-Beom Kwon, Young-Rae Lee, Hyun Jo Youn
Casein kinase 2 (CK2) is a serine/threonine protein kinase that has been considered to represent an important factor in mammary tumorigenesis. Increased expression of matrix metalloproteinase‑9 (MMP‑9) via nuclear factor‑κB (NF‑κB) activation has been demonstrated to promote breast cancer cell invasion. In the present study, the involvement of CK2 in protein kinase C (PKC) induced cell invasion in MCF‑7 breast cancer cells was investigated as well as the underlying molecular mechanisms. The mRNA and protein levels of MMP‑9 in MCF‑7 cells were investigated using reverse transcription‑quantitative polymerase chain reaction, western blot analyses and a zymography assay...
April 13, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29655911/antinociceptive-activity-of-riparin-ii-from-aniba-riparia-further-elucidation-of-the-possible-mechanisms
#16
Alyne Mara Rodrigues de Carvalho, Leonardo Freire Vasconcelos, Nayrton Flávio Moura Rocha, Emiliano Ricardo Vasconcelos Rios, Marília Leite Dias, Marta Maria de França Fonteles, Danielle Macêdo Gaspar, José Maria Barbosa Filho, Stanley Juan Chavez Gutierrez, Francisca Cléa Florenço de Sousa
Riparin II (RipII) has an anti-inflammatory activity potentially due its ability to decrease TNF-α and IL-1β production and its histamine antagonism. The objective of this study was to evaluate the role of RipII in the pain process and the possible antinociceptive mechanisms involved, using classic models of nociception. Male Swiss mice were used in the assays. Determinate the acute toxicity according to the OECD 425 test guideline. The models used were the acetic acid-, formalin-, hot plate and glutamate-induced nociception...
April 12, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29652915/protein-kinase-d3-modulates-mmp1-and-mmp13-expression-in-human-chondrocytes
#17
Jonathan Baker, Adrian M D Falconer, David J Wilkinson, G Nicholas Europe-Finner, Gary J Litherland, Andrew D Rowan
Many catabolic stimuli, including interleukin-1 (IL-1) in combination with oncostatin M (OSM), promote cartilage breakdown via the induction of collagen-degrading collagenases such as matrix metalloproteinase 1 (MMP1) and MMP13 in human articular chondrocytes. Indeed, joint diseases with an inflammatory component are characterised by excessive extracellular matrix (ECM) catabolism. Importantly, protein kinase C (PKC) signalling has a primary role in cytokine-induced MMP1/13 expression, and is known to regulate cellular functions associated with pathologies involving ECM remodelling...
2018: PloS One
https://www.readbyqxmd.com/read/29651299/a-leukocyte-activation-test-identifies-food-items-which-induce-release-of-dna-by-innate-immune-peripheral-blood-leucocytes
#18
Irma Garcia-Martinez, Theresa R Weiss, Muhammad N Yousaf, Ather Ali, Wajahat Z Mehal
Background: Leukocyte activation (LA) testing identifies food items that induce a patient specific cellular response in the immune system, and has recently been shown in a randomized double blinded prospective study to reduce symptoms in patients with irritable bowel syndrome (IBS). We hypothesized that test reactivity to particular food items, and the systemic immune response initiated by these food items, is due to the release of cellular DNA from blood immune cells. Methods: We tested this by quantifying total DNA concentration in the cellular supernatant of immune cells exposed to positive and negative foods from 20 healthy volunteers...
2018: Nutrition & Metabolism
https://www.readbyqxmd.com/read/29649551/the-receptor-for-activated-c-kinase-1-rack1-mediating-immune-response-in-thick-shell-mussel-mytilus-coruscus
#19
Weixiao Lu, Baoying Guo, Xiaoyan Wang, Kaida Xu, Pengzhi Qi
The receptor for activated C kinase 1 (RACK1) is a intracellular receptor for the protein kinase C family which mediates various biological processes. Here, a novel RACK1 gene termed Mc-RACK1 was identified from thick shell mussel, Mytilus coruscus. Mc-RACK1 shared typical RACK1 domains containing WD repeats, PKC phosphorylation sites, N-myristoylation sites, PKC activation sites, TK phosphorylation site and WD motifs. Mc-RACK1 was constitutively expressed in all examined tissues, and its expression in gills, haemocytes and digestive glands were significantly up-regulated upon LPS challenge...
April 9, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29648633/inhibition-of-a-type-k-channels-by-urotensin-ii-induces-sensory-neuronal-hyperexcitability-through-the-pkc%C3%AE-erk-pathway
#20
Yuan Zhang, Hua Wang, Jin Ke, Yuan Wei, Heyi Ji, Zhiyuan Qian, Li Liu, Jin Tao
Previous studies have implicated urotensin-II in the nociception of sensory neurons. However, to date, the relevant mechanisms still remain unknown. In the present study, we determined the role of urotensin-II in the regulation of transient outward A-type potassium currents (IA) as well as neuronal excitability in trigeminal ganglion (TG) neurons. We found that application of urotensin-II to small-diameter TG neurons decreased IA in a dose-dependent manner, while the delayed-rectifier potassium channel current (IDR) was unaffected...
April 10, 2018: Endocrinology
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