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https://www.readbyqxmd.com/read/29145896/downregulation-of-mir-31-stimulates-expression-of-lats2-via-the-hippo-pathway-and-promotes-epithelial-mesenchymal-transition-in-esophageal-squamous-cell-carcinoma
#1
Yanping Gao, Jun Yi, Kai Zhang, Fan Bai, Bing Feng, Rui Wang, Xiaoyuan Chu, Longbang Chen, Haizhu Song
BACKGROUND: Dysregulation of miRNAs is associated with cancer development by coordinately suppressing abundant target genes. Emerging evidence indicates that miR-31 plays a dual role in tumorigenicity. However, whether miR-31 plays as an oncogene in esophageal squamous cell carcinoma (ESCC) and the potential target molecules are still unclear. MiR-31 role in ESCC was investigated and an association of the target molecules with EMT was identified in the progression of ESCC. METHODS: Western blot assays and qRT-PCR was performed to detect the protein and mRNA levels...
November 16, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29136461/enzyme-free-nucleic-acid-amplification-assay-using-a-cellphone-based-well-plate-fluorescence-reader
#2
Donghyuk Kim, Qingshan Wei, Dong Hyeok Kim, Derek Tseng, Jingzi Zhang, Eric Pan, Omai Brandt Garner, Aydogan Ozcan, Dino Di Carlo
Nucleic acids, DNA and RNA, provide important fingerprint information for various pathogens and have significant diag-nostic value; however, improved approaches are urgently needed to enable rapid detection of nucleic acids in simple point-of-care formats with high sensitivity and specificity. Here, we present a system that utilizes a series of toehold-triggered hy-bridization/displacement reactions that are designed to convert a given amount of RNA molecules (i.e., the analyte) into an amplified amount of signaling molecules without any washing steps or thermocycling...
November 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29133396/repression-of-mir-31-by-bcl6-stabilizes-the-helper-function-of-human-follicular-helper-t-cells
#3
A Ripamonti, E Provasi, M Lorenzo, M De Simone, V Ranzani, S Vangelisti, S Curti, R J P Bonnal, L Pignataro, S Torretta, J Geginat, G Rossetti, M Pagani, S Abrignani
Follicular helper T cells (TFHs) are a key component of adaptive immune responses as they help antibody production by B cells. Differentiation and function of TFH cells are controlled by the master gene BCL6, but it is largely unclear how this transcription repressor specifies the TFH program. Here we asked whether BCL6 controlled helper function through down-regulation of specific microRNAs (miRNAs). We first assessed miRNA expression in TFH cells and defined a TFH-specific miRNA signature. We report that hsa-miR-31-5p (miR-31) is down-regulated in TFH; we showed that BCL6 suppresses miR-31 expression by binding to its promoter; and we demonstrated that miR-31 inhibits the expression of molecules that control T-helper function, such as CD40L and SAP...
November 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29115941/microrna-193a-3p-is-specifically-down-regulated-and-acts-as-a-tumor-suppressor-in-braf-mutated-colorectal-cancer
#4
Hidekazu Takahashi, Masanobu Takahashi, Shinobu Ohnuma, Michiaki Unno, Yuki Yoshino, Kota Ouchi, Shin Takahashi, Yasuhide Yamada, Hideki Shimodaira, Chikashi Ishioka
BACKGROUND: The aim of this study was to identify miRNAs specifically dysregulated in BRAF-mutated colorectal cancer, which could lead to a better understanding of the molecular mechanisms underlying oncogenesis of this malignant subtype of colorectal cancer. METHODS: Candidate dysregulated miRNAs were selected in genome-wide miRNA expression array analysis using a screening set composed of 15 BRAF-mutated and 15 non-KRAS/BRAF-mutated colorectal cancers. The miRNA expressions were validated in another set of patients...
November 7, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29115526/the-long-non-coding-rna-enst00000547547-reduces-5-fluorouracil-resistance-of-colorectal-cancer-cells-via-competitive-binding-to-microrna-31
#5
Jian Li, Xiaorong Li, Chaoqun Cen, Xiaohui Ai, Changwei Lin, Gui Hu
Colorectal cancer (CRC) is one of the most common cancers and the third leading cause of cancer-related deaths due to its rapid progression and poor prognosis. 5-Fluorouracil (5-FU)-based chemotherapies are the standard treatment for locally advanced CRC. However, a considerable percentage of CRCs have inherent or acquired 5-FU resistance, which critically impedes clinical outcomes. In the present study, we reported that the expression level ENST00000547547 was downregulated in 5-FU-resistant CRC cells in comparison with the parental cells, While rising with the treatment of 5-FU in parental cells...
November 7, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29056521/study-on-the-role-of-hsa-mir-31-5p-in-hypertrophic-scar-formation-and-the-mechanism
#6
X Wang, Y Zhang, B H Jiang, Q Zhang, R P Zhou, L Zhang, Wang Chen
Hypertrophic scar (HS) formation is associated with the fibrosis of fibrocytes caused by excessive extracellular matrix (ECM) synthesis and deposition, the initial event of HS formation. Our high throughput screen of miRNA expression profiles identified hsa-miR31-5p, whose transcription level was most differentially in normal skin fibroblasts (NS) and HS among other miRNAs. The level of hsa-miR31-5p in HS was significantly higher than in NS. In-vitro functional experiments showed hsa-miR31-5p knockdown remarkably suppressed the proliferation of hypertrophic scar fibroblasts (HSFBs) under hypoxia, promoted cell invasion, and inhibited the expression of Collagen I and III and Fibronectin (FN), suggesting that hsa-miR31-5p knockdown effectively reduces HS formation caused by excessive ECM synthesis and deposition in HSFBs under hypoxia...
October 19, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29054825/integrative-mrna-mirna-interaction-analysis-associate-with-immune-response-of-sea-cucumber-apostichopus-japonicus-based-on-transcriptome-database
#7
Xiaoxu Zhou, Yaqing Chang, Yaoyao Zhan, Xiuli Wang, Kai Lin
MicroRNAs (miRNAs) constitute a family of endogenous non-coding small RNAs that have been demonstrated to be the key effectors in mediating host-pathogen interactions. Additionally, high-throughput sequencing provides unexampled opportunities to identify the pathogenic mechanism underlying miRNAs. In the present study, the target genes of immune-related miRNAs (miR-31, miR-2008, miR-92a, miR-210 and miR-7) and specific miRNAs (miR-2004) in Echinodermata were predicted in silico and validated. Gene ontology (GO) analysis of the target genes of these six miRNAs were conducted to further understand the regulatory function in the host immunity of Apostichopus japonicus (A...
October 18, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29051494/mir-31-promotes-mammary-stem-cell-expansion-and-breast-tumorigenesis-by-suppressing-wnt-signaling-antagonists
#8
Cong Lv, Fengyin Li, Xiang Li, Yuhua Tian, Yue Zhang, Xiaole Sheng, Yongli Song, Qingyong Meng, Shukai Yuan, Liming Luan, Thomas Andl, Xu Feng, Baowei Jiao, Mingang Xu, Maksim V Plikus, Xing Dai, Christopher Lengner, Wei Cui, Fazheng Ren, Jianwei Shuai, Sarah E Millar, Zhengquan Yu
MicroRNA-mediated post-transcriptional regulation plays key roles in stem cell self-renewal and tumorigenesis. However, the in vivo functions of specific microRNAs in controlling mammary stem cell (MaSC) activity and breast cancer formation remain poorly understood. Here we show that miR-31 is highly expressed in MaSC-enriched mammary basal cell population and in mammary tumors, and is regulated by NF-κB signaling. We demonstrate that miR-31 promotes mammary epithelial proliferation and MaSC expansion at the expense of differentiation in vivo...
October 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/29032608/microrna-profiling-reveals-dysregulated-micrornas-and-their-target-gene-regulatory-networks-in-cemento-ossifying-fibroma
#9
Thaís Dos Santos Fontes Pereira, João Artur Ricieri Brito, André Luiz Sena Guimarães, Carolina Cavaliéri Gomes, Júlio Cesar Tanos de Lacerda, Wagner Henriques de Castro, Roney Santos Coimbra, Marina Gonçalves Diniz, Ricardo Santiago Gomez
BACKGROUND: Cemento-ossifying fibroma (COF) is a benign fibro-osseous neoplasm of uncertain pathogenesis, and its treatment results in morbidity. MicroRNAs (miRNA) are small non-coding RNAs that regulate gene expression and may represent therapeutic targets. The purpose of the study was to generate a comprehensive miRNA profile of COF compared to normal bone. Additionally, the most relevant pathways and target genes of differentially expressed miRNA were investigated by in silico analysis...
October 15, 2017: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/28993685/microrna-profiling-in-lung-tissue-and-bronchoalveolar-lavage-of-cigarette-smoke-exposed-mice-and-in-copd-patients-a-translational-approach
#10
Griet Conickx, Francisco Avila Cobos, Maarten van den Berge, Alen Faiz, Wim Timens, Pieter S Hiemstra, Guy F Joos, Guy G Brusselle, Pieter Mestdagh, Ken R Bracke
Chronic obstructive pulmonary disease (COPD) is characterized by a progressive airflow limitation and is associated with a chronic inflammatory response in both airways and lungs. microRNAs (miRNAs) are often highly conserved between species and have an intricate role within homeostatic conditions and immune responses. Also, miRNAs are dysregulated in smoking-associated diseases. We investigated the miRNA profile of 523 miRNAs by stem-loop RT-qPCR in lung tissue and cell-free bronchoalveolar lavage (BAL) supernatant of mice exposed to air or cigarette smoke (CS) for 4 or 24 weeks...
October 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28963737/long-non-coding-rna-loc554202-modulates-chordoma-cell-proliferation-and-invasion-by-recruiting-ezh2-and-regulating-mir-31-expression
#11
Xianli Ma, Shengjin Qi, Zhenying Duan, Hongzhan Liao, Baohua Yang, Wenbo Wang, Jie Tan, Qinghua Li, Xuewei Xia
OBJECTIVES: Chordoma is a rare malignant bone tumour arising from notochordal remnants. Long non-coding RNA LOC554202, as the host gene of miR-31, contributes to various cancer developments. However, little is known about the biological function of LOC554202 in chordoma. Here, the relationship between LncRNA LOC554202, miR-31 and EZH2 was elucidated in chordoma. MATERIALS AND METHODS: The levels of LOC554402, miR-31, EZH2, RNF144B, and epithelial-mesenchymal transition (EMT) markers were measured in chordoma tissues and the chordoma cell lines via quantitative real-time PCR (qRT-PCR) or Western blot...
September 30, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28960215/evaluation-of-the-plasma-microrna-levels-in-stage-3-premature-retinopathy-with-plus-disease-preliminary-study
#12
T Metin, E Dinç, A Görür, S Erdoğan, S Ertekin, A A Sarı, L Tamer, Y Çelik
PurposeIn the present study, we aimed to investigate the changes in plasma microRNA (miRNA) levels in premature infants diagnosed with premature retinopathy (ROP).Patients and methodsIn order to investigate the relationship of miRNAs with ROP, 13 premature infants admitted to Mersin University, Medical School, Department of Ophthalmology and diagnosed with ROP stage 3 with plus disease between January 2014-January 2015 were included in the study. Control group consisted of 15 premature infants with no ROP. The plasma miRNA levels were evaluated using high-throughput quantitative real-time PCR...
September 29, 2017: Eye
https://www.readbyqxmd.com/read/28953910/potential-mirna-involvement-in-the-anti-adipogenic-effect-of-resveratrol-and-its-metabolites
#13
Itziar Eseberri, Arrate Lasa, Jonatan Miranda, Ana Gracia, Maria P Portillo
OBJECTIVE: Scientific research is constantly striving to find molecules which are effective against excessive body fat and its associated complications. Taking into account the beneficial effects that resveratrol exerts on other pathologies through miRNA, the aim of the present work was to analyze the possible involvement of miRNAs in the regulation of adipogenic transcription factors peroxisome proliferator-activated receptor γ (pparγ), CCAAT enhancer-binding proteins α and β (cebpβ and cebpα) induced by resveratrol and its metabolites...
2017: PloS One
https://www.readbyqxmd.com/read/28941018/role-of-non-coding-rnas-in-head-and-neck-squamous-cell-carcinoma-a-narrative-review
#14
REVIEW
M K Sannigrahi, Rajni Sharma, Naresh K Panda, Madhu Khullar
Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide with high recurrence, metastasis, and poor treatment outcome. Recent studies have reported that non-coding RNA (ncRNA) might play critical role in regulating different types of cancer. MicroRNAs (miRs) are short ncRNAs (20-25 nucleotides), responsible for post-transcriptional regulation of gene expression and may have a role in oncogenesis by acting as oncomiRs or tumor-suppressors miRs. Long non-coding RNAs (lncRNAs) are heterogenous group of ncRNAs more than 200 nucleotides long, can act in cis and/or in trans, and, have been also implicated in carcinogenesis...
September 21, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28937251/roles-of-mir-31-and-endothelin-1-in-psoriasis-vulgaris-pathophysiological-functions-and-potential-biomarkers
#15
L Borska, C Andrys, M Chmelarova, H Kovarikova, J Krejsek, K Hamakova, M Beranek, V Palicka, J Kremlacek, P Borsky, Z Fiala
Psoriatic lesions are characterized by hyperproliferation, aberrant differentiation of keratinocytes resistant to apoptosis and inflammation. miR-31 plays pro-proliferative, pro-differentiative and pro-inflammatory roles and modulates apoptosis in psoriatic keratinocytes. Endothelin 1 (ET-1) is produced by psoriatic keratinocytes and suppresses apoptosis. Inflammation increases the production of ET-1, which in turn leads to the chronic stimulation of keratinocyte proliferation. The aim of this study was to identify the putative link between two potential biomarkers (miR-31 and ET-1) in patients with psoriasis...
September 22, 2017: Physiological Research
https://www.readbyqxmd.com/read/28927157/selected-mirna-levels-are-associated-with-ikzf1-microdeletions-in-pediatric-acute-lymphoblastic-leukemia
#16
J Krzanowski, J Madzio, A Pastorczak, A Tracz, M Braun, J Tabarkiewicz, A Pluta, W Młynarski, I Zawlik
The clinical outcome of children with high-risk relapsed B-cell precursor acute lymphoblastic leukemia (BCP-ALL) is poor. The present study assessed the utility and prognostic value of selected microRNA (miRNA/miR) in BCP-ALL. The changes in the expression levels of these miRNAs regarding known gene lesions affecting lymphoid development [early B-cell factor 1 (EBF1), ETS variant 6 (ETV6), IKAROS family zinc finger 1 (IKZF1), paired box 5 (PAX5), cyclin dependent kinase inhibitor (CDKN) 2A/CDKN2B, retinoblastoma 1 (RB1), pseudoautosomal region 1 (PAR1), B-cell translocation gene 1 protein (BTG1)] were analyzed...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28926530/erratum-the-microrna-mir-31-inhibits-cd8-t-cell-function-in-chronic-viral-infection
#17
Howell F Moffett, Adam N R Cartwright, Hye-Jung Kim, Jernej Godec, Jason Pyrdol, Tarmo Äijö, Gustavo J Martinez, Anjana Rao, Jun Lu, Todd R Golub, Harvey Cantor, Arlene H Sharpe, Carl D Novina, Kai W Wucherpfennig
No abstract text is available yet for this article.
September 19, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28918032/microrna-31-regulates-chemosensitivity-in-malignant-pleural-mesothelioma
#18
Hannah L Moody, Michael J Lind, Stephen G Maher
Malignant pleural mesothelioma (MPM) is associated with an extremely poor prognosis, and most patients initially are or rapidly become unresponsive to platinum-based chemotherapy. MicroRNA-31 (miR-31) is encoded on a genomic fragile site, 9p21.3, which is reportedly lost in many MPM tumors. Based on previous findings in a variety of other cancers, we hypothesized that miR-31 alters chemosensitivity and that miR-31 reconstitution may influence sensitivity to chemotherapeutics in MPM. Reintroduction of miR-31 into miR-31 null NCI-H2452 cells significantly enhanced clonogenic resistance to cisplatin and carboplatin...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28916770/changes-in-cd73-cd39-and-cd26-expression-on-t-lymphocytes-of-anca-associated-vasculitis-patients-suggest-impairment-in-adenosine-generation-and-turn-over
#19
Lovis Kling, Urs Benck, Annette Breedijk, Lisa Leikeim, Marianne Heitzmann, Stefan Porubsky, Bernhard K Krämer, Benito A Yard, Anna-Isabelle Kälsch
Extracellular adenosine, generated via the concerted action of CD39 and CD73, contributes to T-cell differentiation and function. Adenosine concentrations are furthermore influenced by adenosine deaminase binding protein CD26. Because aberrant T-cell phenotypes had been reported in anti-neutrophil cytoplasmic auto-antibody (ANCA)-associated vasculitis (AAV) patients, an impaired expression of these molecules on T-cells of AAV patients was hypothesized in the present study. While in AAV patients (n = 29) CD26 was increased on CD4(+) lymphocytes, CD39 and CD73 were generally reduced on patients' T-cells...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28901412/differential-mirna-expression-profiles-in-human-keratinocytes-in-response-to-protein-kinase-c-inhibitor
#20
Yang Liu, Liangchen Zhong, Dewu Liu, Hua Ye, Yuangui Mao, Yanghong Hu
Aberrant expression of microRNAs (miRNAs) is widely accepted to be involved in keratinocyte differentiation and to be dependent on activation of the protein kinase C (PKC) pathway. However, the miRNA profiles and biological characteristics of keratinocytes induced by specific inhibitors of PKC have yet to be elucidated. The present study aimed to explore the differential miRNA expression profiles in keratinocytes treated with the PKC inhibitor GF109203X, by conducting a bioinformatics analysis. Parts of the GF109203X‑induced keratinocytes formed distinct clones after 2 days of culture, and the expression of intergrin β1, cytokeratin (CK)19 and CK14 were positive, whereas CK10 expression was negative...
November 2017: Molecular Medicine Reports
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