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Tami Yap, Kendrick Koo, Lesley Cheng, Laura J Vella, Andrew F Hill, Eric C Reynolds, Alf L Nastri, Nicola Cirillo, Christine A Seers, Michael McCullough
Oral swirls are a noninvasive, rapidly collected source of salivary microRNA potentially useful in the early detection of disease states, particularly oral squamous cell carcinoma (OSCC). The aim of this study was to predict the presence of oral squamous cell carcinoma using a panel of OSCC-related dysregulated microRNA found in oral swirls, identified jointly in data from formalin-fixed paraffin embedded (FFPE) and fresh frozen specimens. Next generation sequencing (NGS) was used to determine microRNA fold changes in FFPE OSCC specimens relative to histologically normal epithelium...
May 15, 2018: Cancer Prevention Research
Mariah L Hoye, Angela S Archambault, Taylor M Gordon, Landon K Oetjen, Matthew D Cain, Robyn S Klein, Seth D Crosby, Brian S Kim, Timothy M Miller, Gregory F Wu
Innate immune cells are integral to the pathogenesis of several diseases of the central nervous system (CNS), including multiple sclerosis (MS). Dendritic cells (DCs) are potent CD11c+ antigen-presenting cells that are critical regulators of adaptive immune responses, particularly in autoimmune diseases such as MS. The regulation of DC function in both the periphery and CNS compartment has not been fully elucidated. One limitation to studying the role of CD11c+ DCs in the CNS is that microglia can upregulate CD11c during inflammation, making it challenging to distinguish bone marrow-derived DCs (BMDCs) from microglia...
May 10, 2018: Immunology
Lidia Ruiz-Llorente, Constanza Contreras-Jurado, Mónica Martínez-Fernández, Jesus Maria Paramio, Ana Aranda
BACKGROUND: MicroRNAs (miRNAs) play a unique role in posttranscriptional regulation of gene expression and control different aspects of skin development, homeostasis and disease. Although it is generally accepted that thyroid hormones signaling is important in skin pathophysiology, the role of their nuclear receptors (TRs) in cutaneous miRNA expression has yet to be explored. METHODS: We have used RNAseq to compare the skin miRnome of wild-type mice and genetically modified mice lacking both TRα1 and TRβ, the main thyroid hormone binding isoforms...
May 10, 2018: Thyroid: Official Journal of the American Thyroid Association
Osamu Ishibashi, Ichiro Akagi, Yota Ogawa, Takashi Inui
The phosphatidylinositol-3-kinase (PI3K)/AKT pathway is frequently activated in various human cancers and plays essential roles in their development and progression. Accumulating evidence suggests that dysregulated expression of microRNAs (miRNAs) is closely associated with cancer progression and metastasis. Here, we focused on miRNAs that could regulate genes related to the PI3K/AKT pathway in esophageal squamous cell carcinoma (ESCC). To identify upregulated miRNAs and their possible target genes in ESCC, we performed microarray-based integrative analyses of miRNA and mRNA expression levels in three human ESCC cell lines and a normal esophageal epithelial cell line...
May 11, 2018: Biochemical and Biophysical Research Communications
Shujuan Yang, Jing Wang, Wensheng Ge, Yanfang Jiang
Laryngeal squamous cell carcinoma (LSCC) is one aggressive malignancy and accounts for 20% of all head and neck cancer. However, the role of LOC554202 in human LSCC remains unknown. The expression level of LOC554202 and miR-31 was detected in the LSCC tiussues by using qRT-PCR. Cell growth was measured by CCK-8 assay. Flow cytometry and matrigel-coated membrane was used to detect for cell cycle and invasion respectively. We indicated that lncRNA LOC554202 expression was overexpressed in LSCC tissues compared with the paired adjacent samples and higher LOC554202 expression was associated with the advanced stage...
May 8, 2018: Journal of Cellular Biochemistry
David C Hughes, George R Marcotte, Leslie M Baehr, Daniel W D West, Andrea G Marshall, Scott M Ebert, Arik Davidyan, Christopher M Adams, Sue C Bodine, Keith Baar
KEY POINTS: Force transfer is integral for maintaining skeletal muscle structure and function. One important component is dystrophin. There is limited understanding of how force transfer is impacted by age and loading. Here, we investigate the force transfer apparatus in muscles of adult and old rats exposed to periods of disuse and reloading. Our results demonstrate an increase in dystrophin protein during the reloading phase in the adult TA muscle that is delayed in old. The consequence of this delay is an increased susceptibility towards contraction-induced muscle injury...
May 3, 2018: Journal of Physiology
Jessica S Kurata, Ren-Jang Lin
MicroRNAs (miRNAs) are posttranscriptional gene regulators that play important roles in the control of cell fitness, differentiation, and development. The CRISPR-Cas9 gene-editing system is composed of the Cas9 nuclease in complex with a single guide RNA (sgRNA) and directs DNA cleavage at a predetermined site. Several CRISPR-Cas9 libraries have been constructed for genome-scale knockout screens of protein function; however few libraries have included miRNA genes. Here we constructed a miRNA-focused CRISPR-Cas9 library that targets 1,594 (85%) annotated human miRNA stem-loops...
May 2, 2018: RNA
Marine Guilbaud, Christel Gentil, Cécile Peccate, Elena Gargaun, Isabelle Holtzmann, Carole Gruszczynski, Sestina Falcone, Kamel Mamchaoui, Rabah Ben Yaou, France Leturcq, Laurence Jeanson-Leh, France Piétri-Rouxel
BACKGROUND: Duchenne (DMD) and Becker (BMD) muscular dystrophies are caused by mutations in the DMD gene coding for dystrophin, a protein being part of a large sarcolemmal protein scaffold that includes the neuronal nitric oxide synthase (nNOS). The nNOS was shown to play critical roles in a variety of muscle functions and alterations of its expression and location in dystrophic muscle fiber leads to an increase of the muscle fatigability. We previously revealed a decrease of nNOS expression in BMD patients all presenting a deletion of exons 45 to 55 in the DMD gene (BMDd45-55), impacting the nNOS binding site of dystrophin...
April 27, 2018: Skeletal Muscle
C D'Aniello, S Pisconti, S Facchini, C Imbimbo, C Cavaliere
No abstract text is available yet for this article.
April 2018: European Review for Medical and Pharmacological Sciences
Peng Cai, Hongxing Li, Weiwei Huo, Hairong Zhu, Chao Xu, Rujin Zang, Wei Lv, Yankai Xia, Weibing Tang
Hirschsprung's disease (HSCR) is a birth defect that causes a failure of the enteric nervous system to cover the distal gut during early embryonic development. Evidence shows that long non-coding RNAs (lncRNA) play important roles in HSCR. The MIR31 host gene (MIR31HG), also known as Loc554202, is a long non-coding RNA (lncRNA), which acts as the host gene of (microRNA) miR-31 and miR-31*. There have been no studies regarding its function in early developmental defects during pregnancy, and its downstream genetic receptors...
April 6, 2018: Journal of Cellular Biochemistry
Pascal Gottmann, Meriem Ouni, Sophie Sausenthaler, Julian Roos, Laura Stirm, Markus Jähnert, Anne Kamitz, Nicole Hallahan, Wenke Jonas, Andreas Fritsche, Hans-Ulrich Häring, Harald Staiger, Matthias Blüher, Pamela Fischer-Posovszky, Heike Vogel, Annette Schürmann
OBJECTIVE: Obesity and type 2 diabetes (T2D) arise from the interplay between genetic, epigenetic, and environmental factors. The aim of this study was to combine bioinformatics and functional studies to identify miRNAs that contribute to obesity and T2D. METHODS: A computational framework (miR-QTL-Scan) was applied by combining QTL, miRNA prediction, and transcriptomics in order to enhance the power for the discovery of miRNAs as regulative elements. Expression of several miRNAs was analyzed in human adipose tissue and blood cells and miR-31 was manipulated in a human fat cell line...
March 15, 2018: Molecular Metabolism
Jianyun Shi, Xianghui Ma, Yang Su, Yongli Song, Yuhua Tian, Shukai Yuan, Xiuqing Zhang, Dong Yang, Hao Zhang, Jianwei Shuai, Wei Cui, Fazheng Ren, Maksim V Plikus, Yaoxing Chen, Jie Luo, Zhengquan Yu
Wound healing is essential for skin repair after injury, and consists of hemostasis, inflammation, re-epithelialization and remodeling phases. Successful re-epithelialization, which relies on proliferation and migration of epidermal keratinocytes, requires reduction in tissue inflammation. Therefore, understanding the molecular mechanism underlying the transition from inflammation to re-epithelialization will help to better understand the principles of wound healing. Currently, the in vivo functions of specific microRNAs in wound healing are not fully understood...
March 29, 2018: Journal of Investigative Dermatology
Qiao Yi Chen, Jinquan Li, Hong Sun, Feng Wu, Yusha Zhu, Thomas Kluz, Ashley Jordan, Thomas DesMarais, Xiaoru Zhang, Anthony Murphy, Max Costa
Arsenic is a naturally occurring and highly potent metalloid known to elicit serious public health concerns. Today, approximately 200 million people around the globe are exposed to arsenic-contaminated drinking water at levels greater than the World Health Organization's recommended limit of 10 parts per billion. As a class I human carcinogen, arsenic exposure is known to elicit various cancers, including lung, skin, liver, and kidney. Current evidence suggests that arsenic is capable of inducing both genotoxic and cytotoxic injury, as well as activating epigenetic pathways to induce carcinogenesis...
March 30, 2018: Molecular Carcinogenesis
Lucas Ramon, Catherine David, Karine Fontaine, Elodie Lallet, Charles Marcaillou, Séverine Martin-Lannerée, Virginie Decaulne, Céline Vazart, Anne-Héloise Gélibert, Raouf Ben Abdelali, Jean-Marc Costa, Francis Rousseau, Raphaële Thiébaut, Larry Yost, Yann Gaston-Mathé
MiR-31-3p expression has been shown to be a predictive biomarker for response to anti-epithelial growth factor receptor therapy in patients with RAS wild-type metastatic colorectal cancer (mCRC). To aid in the quantification of miR-31-3p expression in formalin-fixed paraffin-embedded (FFPE) primary tumor samples from patients with mCRC, a reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assay was developed and validated. Assay development included the identification of a microRNA reference standard and the determination of an appropriate relative quantification cutoff for differentiating low versus high miR-31-3p expression...
2018: Biomarker Insights
Weishuai Lian, Xiaoxiao Hu, Rongfeng Shi, Shilong Han, Chuanwu Cao, Kun Wang, Maoquan Li
Endothelial malfunctions in patients with diabetes are known to result in vascular diseases, and endothelial progenitor cells (EPCs) are indispensable for the functional preservation of the vascular endothelium. MicroRNA-31 (miR-31) has been found to be able to modulate the differentiation of stem cells. However, it is still unclear how miR-31 functions in diabetic EPCs. The aim of this study was to investigate how miR-31 regulates diabetic EPC function. In the current study, miR-31 expression was compared between normal and diabetic EPCs...
March 16, 2018: Acta Biochimica et Biophysica Sinica
Ryota Kurogi, Akira Nakamizo, Satoshi O Suzuki, Masahiro Mizoguchi, Koji Yoshimoto, Toshiyuki Amano, Takeo Amemiya, So Takagishi, Koji Iihara
OBJECTIVE Human bone marrow-derived mesenchymal stem cells (hMSCs) show tropism for brain tumors and may be a useful vehicle for drug or gene delivery to malignant gliomas. Recently, some microRNAs (miRNAs) have been shown to suppress the invasiveness of malignant gliomas. METHODS To test their potential to become vehicles for the delivery of miRNA to malignant gliomas, hMSCs were engineered so that hMSC secretion of miRNAs that inhibit glioma cell invasion was enabled without altering the hMSC tropism for glioma cells...
March 9, 2018: Journal of Neurosurgery
Riikka E Mäkitie, Matthias Hackl, Riitta Niinimäki, Sakari Kakko, Johannes Grillari, Outi Mäkitie
Context: WNT signaling is fundamental to bone health, and its aberrant activation leads to skeletal pathologies. The heterozygous missense mutation p.C218G in WNT1, a key WNT pathway ligand, leads to severe early-onset and progressive osteoporosis with multiple peripheral and spinal fractures. Despite the severe skeletal manifestations, conventional bone turnover markers are normal in mutation-positive patients. Objective: This study sought to explore the circulating microRNA (miRNA) pattern in patients with impaired WNT signaling...
May 1, 2018: Journal of Clinical Endocrinology and Metabolism
Chen Chen, Shenfei Zong, Zhuyuan Wang, Ju Lu, Dan Zhu, Yizhi Zhang, Ruohu Zhang, Yiping Cui
Exosomes are small membrane vesicles secreted by a wide variety of cells. Studies have demonstrated that exosomal miRNAs can influence the biological processes of recipient cells. Therefore, direct imaging and tracking of exosomal miRNAs in living recipient cells are essential for exosome functional analysis. However, the moderate spatial resolution of conventional fluorescence microscopy limits the precise imaging and tracking of exosomes considering their relatively small size (<100 nm). Here, we took advantage of single molecule localization microscopy (SMLM) to realize the visualization and dynamic tracking of exosomes and exosomal miRNAs in living cells...
March 15, 2018: Nanoscale
Li Zhang, Cuiping Wang, Shuangni Yu, Congwei Jia, Jie Yan, Zhaohui Lu, Jie Chen
Mutations in the AT-rich interactive domain 1A gene, which encodes a subunit of the Switch/Sucrose nonfermentable chromatin remodeling complex, can result in loss of protein expression and are associated with different cancers. Here, we used immunohistochemistry to investigate the significance of AT-rich interactive domain 1A loss in 73 pancreatic ductal adenocarcinoma cases with paired paracancerous normal pancreatic tissues. The relationship between levels of the AT-rich interactive domain 1A protein product, BAF250a, and clinicopathological parameters in the 73 pancreatic cancer specimens was also analyzed...
January 1, 2018: Technology in Cancer Research & Treatment
Katarina Zeljic, Ivan Jovanovic, Jasmina Jovanovic, Zvonko Magic, Aleksandra Stankovic, Gordana Supic
AIM: It was the aim of the study to identify commonly deregulated miRNAs in oral cancer patients by performing a meta-analysis of previously published miRNA expression profiles in cancer and matched normal non-cancerous tissue in such patients. MATERIAL AND METHODS: Meta-analysis included seven independent studies analyzed by a vote-counting method followed by bioinformatic enrichment analysis. RESULTS: Amongst seven independent studies included in the meta-analysis, 20 miRNAs were found to be deregulated in oral cancer when compared with non-cancerous tissue...
March 2018: Upsala Journal of Medical Sciences
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