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Shuyao Tang, Gang Tan, Xian Jiang, Peng Han, Bo Zhai, Xuesong Dong, Haiquan Qiao, Hongchi Jiang, Xueying Sun
Sorafenib resistance remains a major obstacle for the effective treatment of hepatocellular carcinoma (HCC), and a number of miRNAs contribute to this resistance. However, the regulatory networks of miRNAs are very complex, thus inhibiting a single miRNA may sequentially activate other compensatory pathways. In the present study, we generated an artificial long non-coding RNA (AlncRNA), which simultaneously targets multiple miRNAs including miR-21, miR-153, miR-216a, miR-217, miR-494 and miR-10a-5p. These miRNAs have been shown to be upregulated in sorafenib-resistant cells and participate in the mechanisms underlying sorafenib resistance...
September 28, 2016: Oncotarget
Ajay Kumar Dixit, Anne E Sarver, Zuobiao Yuan, John George, Usman Barlass, Hassam Cheema, Archana Sareen, Sulagna Banerjee, Vikas Dudeja, Rajinder K Dawra, Subbaya Subramanian, Ashok K Saluja
In the current study, we have characterized the global miRNA expression profile in mouse pancreatic acinar cells and during acute pancreatitis using next-generation RNA-Sequencing. We identified 324 known and 6 novel miRNAs being expressed in mouse pancreatic acinar cells. At basal state, miR-148a-3p, miR-375-3p, miR-217-5p, miR-200a-3p were among the most abundantly expressed whereas, miR-24-5p and miR-421-3p were least abundant. Treatment of acinar cells with caerulein (100 nM) and taurolithocholic acid 3-sulfate [TLC-S (250 μM)] induced numerous changes in miRNA expression profile...
September 29, 2016: American Journal of Physiology. Gastrointestinal and Liver Physiology
Ana Clara P Azevedo-Pouly, Dhruvitkumar S Sutaria, Jinmai Jiang, Ola A Elgamal, Foued Amari, David Allard, Paul J Grippo, Vincenzo Coppola, Thomas D Schmittgen
Mice harboring a G12D activating Kras mutation are among the most heavily studied models in the field of pancreatic adenocarcinoma (PDAC) research. miRNAs are differentially expressed in PDAC from patients and mouse models of PDAC. To better understand the relationship that Kras activation has on miRNA expression, we profiled the expression of 629 miRNAs in RNA isolated from the pancreas of control, young, and old P48(+/Cre);LSL-KRAS(G12D) as well as PDX-1-Cre;LSL-KRAS(G12D) mice. One hundred of the differentially expressed miRNAs had increased expression in the advanced disease (old) P48(+/Cre);LSL-KRAS(G12D) compared to wild-type mice...
August 19, 2016: Functional & Integrative Genomics
Ying Shao, Huiwen Ren, Chuan Lv, Xiaoyu Ma, Can Wu, Qiuyue Wang
The aim of this study is to investigate the correlation between serum microRNA-217 and the severity of diabetic kidney disease determined by albuminuria. Four hundred ninety five type 2 diabetes patients were divided into three groups: normoalbuminuric group, microalbuminuric group, and macroalbuminuric group. Serum microRNA-217 levels were validated by real-time polymerase chain reaction. Serum silent information regulator 1, Hypoxia-inducible factor-1α and vascular endothelial growth factor were determined by enzyme-linked immunosorbent assay...
August 13, 2016: Endocrine
Jacqueline Calvano, Gwendolyn Edwards, Clifford Hixson, Holly Burr, Raja Mangipudy, Mark Tirmenstein
Pancreatic injury in rats is primarily detected through histopathological changes and conventional serum biomarkers such as amylase and lipase. However, amylase and lipase have a short half-life and are markers of acinar, not islet cell injury. We investigated whether circulating microRNA (miR) levels that are enriched in acinar cells (miR-217, miR-216a/b) or islet cells (miR-375) could serve as markers of pancreatic injury. Rats were treated with a single dose of either vehicle, streptozotocin (STZ), caerulein, or acetaminophen (APAP), and necropsied at 4, 24, and 48h...
August 10, 2016: Toxicology
Wei Zhang, Liang Zhong, Jing Wang, Jianyong Han
It is well known that microRNAs play a very important role in regulating reprogramming, pluripotency and cell fate decisions. Porcine induced pluripotent stem cells (piPSCs) are now available for studying the pluripotent regulation network in pigs. Two types of piPSCs have been derived from human and mouse embryonic stem cell (ESC) culture conditions: hpiPSCs and mpiPSCs, respectively. The hpiPSCs were morphologically similar to human ESCs, and the mpiPSCs resembled mouse ESCs. However, our current understanding of the role of microRNAs in the development of piPSCs is still very limited...
2016: PloS One
Hongyan Duan, Yongqiang Li, Lijie Yan, Haitao Yang, Jintao Wu, Peng Qian, Bing Li, Shanling Wang
Hyperhomocysteine has become a critical risk for atherosclerosis and can stimulate proliferation and migration of vascular smooth muscle cells (VSMCs). N-methyl-D-aspartic acid receptor (NMDAR) is a receptor of homocysteine and mediates the effects of homocysteine on VSMCs. Bioinformatics analysis has shown NMDAR is a potential target of microRNA-217 (miR-217), which exerts multiple functions in cancer tumorigenesis and carotid plaque progression. In this study, we sought to investigate the role of miR-217 in VSMCs phenotype transition under homocysteine exposure and elucidate its effect on atherosclerotic plaque formation...
October 2016: Clinical and Experimental Pharmacology & Physiology
Yonggang Zhu, Hongguang Zhao, Li Feng, Songbai Xu
MircroRNA-217 (miR-217) has been showed to involve in the initiation and development of human cancers, and is recognize as a tumor suppressor miRNA in several tumors. However, the clinical significance and its underlying role in human glioma remain unclear. Herein, we found that the expression of miR-217 was significantly down-regulated in glioma tissues as compared with adjacent normal brain tissues. Clinical association analysis disclosed that low-expression of miR-217 was evidently negative associated with advanced tumor stage (grade III + IV) in glioma...
2016: American Journal of Translational Research
Yaron Hillman, Niv Mazkereth, Luba Farberov, Noam Shomron, Zvi Fishelson
The impact of microRNAs (miRNAs) known to regulate numerous biologic processes on complement-dependent cytotoxicity (CDC) was investigated in K562 cells. The C5b-9 complex is the executioner of CDC. Cells protect themselves from CDC by C5b-9 elimination, a process involving the mitochondrial chaperone mortalin/GRP75. Potential miR-200 (b and c) and miR-217 regulatory sites were identified in mortalin mRNA. Overexpression of miR-200b/c or miR-217 lowered the expression of mortalin mRNA. miRNA inhibitors for miR-200b, miR-200c, or miR-217 enhanced mortalin mRNA level...
June 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
Wei Han, Yunfen Zhu, Yijun Su, Guohui Li, Liang Qu, Huiyong Zhang, Kehua Wang, Jianmin Zou, Honglin Liu
There are still no highly sensitive and unique biomarkers for measurement of puberty onset. Circulating miRNAs have been shown to be promising biomarkers for diagnosis of various diseases. To identify circulating miRNAs that could be served as biomarkers for measuring chicken (Gallus gallus) puberty onset, the Solexa deep sequencing was performed to analyze the miRNA expression profiles in serum and plasma of hens from two different pubertal stages, before puberty onset (BO) and after puberty onset (AO). 197 conserved and 19 novel miRNAs (reads > 10) were identified as serum/plasma-expressed miRNAs in the chicken...
2016: PloS One
Unal Egeli, Gulcin Tezcan, Gulsah Cecener, Berrin Tunca, Elif Demirdogen Sevinc, Ekrem Kaya, Secil Ak, Halit Ziya Dundar, Pinar Sarkut, Nesrin Ugras, Omer Yerci, Yilmaz Ozen, Turkkan Evrensel
OBJECTIVES: The success of gemcitabine plus radiotherapy is dependent on the mutation status of pancreatic ductal adenocarcinoma (PDAC) tumors in the EGFR and KRAS genes; however, radiotherapy resistance may also be modulated epigenetically by microRNA (miRNA) regulation. In this study, we examined the potential effect of miRNAs on the resistance to radiotherapy in cases without EGFR or KRAS mutation. METHODS: The association of EGFR and KRAS mutation status and different expression patterns of 6 selected miRNAs related to the EGFR/KRAS signaling pathway were evaluated in the tumors of 42 patients with PDAC...
October 2016: Pancreas
Meng Yang, Guozhong Cui, Mingjian Ding, Wenhua Yang, Yanqing Liu, Dianlu Dai, Liang Chen
Gastric cancer is the most common cancer in the world, miRNAs have been demonstrated to play critical role in the development and progression of gastric cancer, such as miR-7, miR-217 and miR-335. Here, we found miR-935 was upregulated in gastric cancer tissues and cells. Overexpression of miR-935 promoted cell proliferation and tumorigenesis in vitro determined by MTT analysis, colony formation analysis, BrdU cell proliferation analysis and soft agar growth analysis, knockdown of miR-935 reduced these effects...
April 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Guoting Chen, Ning Han, Guofeng Li, Xin Li, Guang Li, Zengchun Li, Qinchuan Li
BACKGROUND: The study aimed to investigate the expression changes of genes in colorectal cancer (CRC) and screen the potential molecular targets. METHODS: The GSE37178 of mRNA expression profile including the CRC samples extracted by surgical resection and the paired normal samples was downloaded from Gene Expression Omnibus database. The genes whose expressions were changed at four different time points were screened and clustered using Mfuzz package. Then DAVID was used to perform the functional and pathway enrichment analysis for genes in different clusters...
2016: Cancer Cell International
Deokbum Park, Hyuna Kim, Youngmi Kim, Dooil Jeoung
We have previously reported the role of miR-217 in anti-cancer drug-resistance. miRNA array and miRNA hybridization analysis predicted miR-30a-3p as a target of miR-217. miR-30a-3p and miR-217 formed a negative feedback loop and regulated the expression of each other. Ago1 immunoprecipitation and co-localization analysis revealed a possible interaction between miR-30a-3p and miR-217. miR-30a-3p conferred resistance to anti-cancer drugs and enhanced the invasion, migration, angiogenic, tumorigenic, and metastatic potential of cancer cells in CAGE-dependent manner...
April 30, 2016: Molecules and Cells
Ying Shao, Chuan Lv, Can Wu, Yuehong Zhou, Qiuyue Wang
BACKGROUND: Silent information regulator 1 (Sirt1) plays a protective role in kidney. Sirt1 suppresses activation of hypoxia-inducible factor-1 alpha (HIF-1α), with MircroRNA-217 (Mir-217) being closely related to Sirt1. The relationship of Sirt1, HIF-1α and Mir-217, however, has never been reported in high glucose cultured rat glomerular mesangial cells (RMCs). Thus, we explored the role of Mir-217 on inflammation and fibrosis in RMCs cultured with high glucose in vitro through Sirt1/HIF-1α signaling pathway...
September 2016: Diabetes/metabolism Research and Reviews
Youngmi Kim, Hyuna Kim, Deokbum Park, Minho Han, Hansoo Lee, Yun Sil Lee, Jongseon Choe, Young Myeong Kim, Dooil Jeoung
MicroRNA array analysis revealed that miR-217 expression was decreased in anti-cancer drug-resistant Malme3MR cancer cells. CAGE, a cancer/testis antigen, was predicted as a target of miR-217. Luciferase activity and ChIP assays revealed a negative feedback relationship between CAGE and miR-217. miR-217 and CAGE oppositely regulated the response to anti-cancer drugs such as taxol, gefitinib and trastuzumab, an inhibitor of HER2. miR-217 negatively regulated the tumorigenic, metastatic, angiogenic, migration and invasion potential of cancer cells...
March 1, 2016: Oncotarget
Lijun Dong, Lina Hui
BACKGROUND: The aim of this study was to determine the effect on proliferation, migration, and invasion after silencing HOTAIR in ovarian cancer SKOV3 cells, and to elucidate the potential mechanism. MATERIAL/METHODS: We analyzed the mRNA expression level of HOTAIR and PIK3R3 in ovarian cancer SKOV3, OVCAR3, and A2780 cell lines. We analyzed the mRNA expression level of HOTAIR and PIK3R3 in ovarian SKOV3 after transection with miR-214 or miR-217. We analyzed the mRNA and protein expression level of PIK3R3 when silencing HOTAIR...
2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Jieyan Li, Dongmei Li, Weiyuan Zhang
Accumulating evidence shows that microRNA-217 (miR-217) is frequently dysregulated in various cancers, and plays crucial roles in tumorigenesis and metastasis; however, the role and underlying molecular mechanism of miR-217 in human epithelial ovarian cancer (EOC) remains unclear. Here, we report that miR-217 expression was downregulated in EOC tissue and inversely correlated with advanced FIGO stage, high histological grading and lymph node metastasis (P<0.01). Function analysis revealed that the ectopic expression of miR-217 in EOC cells inhibited cell proliferation, migration and invasion in vitro, as well as suppressed tumor growth in vivo...
March 2016: Oncology Reports
Ondřej Volný, Linda Kašičková, Dominika Coufalová, Petra Cimflová, Jan Novák
Cardiovascular diseases are major causes of morbidity and mortality in developed countries. Cerebrovascular diseases, especially stroke, represent major burden of disability and economy impact. Major advances in primary and secondary prevention and therapy are needed in order to tackle this public health problem. Our better understanding of pathophysiology is essential in order to develop novel diagnostic and therapeutic tools and strategies. microRNAs are a family of important post-transcriptional regulators of gene expression and their involvement in the pathophysiology of cerebrovascular diseases has already been reported...
2015: Advances in Experimental Medicine and Biology
Liangyou Gu, Hongzhao Li, Luyao Chen, Xin Ma, Yu Gao, Xintao Li, Yu Zhang, Yang Fan, Xu Zhang
This is a systematic review of studies investigating the prognostic value of different microRNAs (miRs) in renal cell carcinoma (RCC). Twenty-seven relevant studies were identified, with a total of 2578 subjects. We found that elevated expression of miR-21, miR-1260b, miR-210, miR-100, miR-125b, miR-221, miR-630, and miR-497 was associated with a poor prognosis in RCC patients. Conversely, decreased expression of miR-106b, miR-99a, miR-1826, miR-215, miR-217, miR-187, miR-129-3p, miR-23b, miR-27b, and miR-126 was associated with a worse prognosis...
October 20, 2015: Oncotarget
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