keyword
https://read.qxmd.com/read/36546322/immature-platelet-fraction-predicts-adverse-events-in-patients-with-acute-coronary-syndrome-the-isar-react-5-reticulated-platelet-substudy
#21
RANDOMIZED CONTROLLED TRIAL
Dario Bongiovanni, Nina Schreiner, Rosanna Gosetti, Katharina Mayer, Dominick J Angiolillo, Dirk Sibbing, Stefan Holdenrieder, Aida Anetsberger, Moritz von Scheidt, Heribert Schunkert, Karl-Ludwig Laugwitz, Stefanie Schüpke, Adnan Kastrati, Isabel Fegers-Wustrow, Isabell Bernlochner
BACKGROUND: Immature or reticulated platelets are associated with impaired efficacy of antiplatelet drugs and adverse events in cardiovascular patients. Their role as a predictive biomarker in patients with acute coronary syndrome treated with potent P2Y12 receptor inhibitors is not fully understood. We aimed to prospectively evaluate reticulated platelets as a predictor of the primary end point of the ISAR-REACT 5 trial consisting of death, myocardial infarction, or stroke at 1 year in patients with acute coronary syndrome randomized to prasugrel or ticagrelor...
February 2023: Arteriosclerosis, Thrombosis, and Vascular Biology
https://read.qxmd.com/read/36362823/reticulated-platelets-and-their-relationship-with-endothelial-progenitor-cells-during-the-acute-phase-of-st-elevation-myocardial-infarction
#22
JOURNAL ARTICLE
Nili Schamroth Pravda, Mark Kheifets, Maya Wiessman, Dorit Leshem-Lev, Hana Vaknin Assa, Ran Kornowski, Yeela Talmor-Barkan, Leor Perl
INTRODUCTION: Endothelial progenitor cells (EPC) and reticulated platelets (RP) have central roles in the thrombotic and angiogenetic interactions during ST-elevation myocardial infarction (STEMI). The EPC and RP response in patients with STEMI treated by primary percutaneous intervention (PPCI) has not yet been investigated. METHODS: We assessed EPC quantification by the expression of CD133+ and CD34+ , and EPC function by the capacity of the cells to form colony-forming units (CFU) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) during the acute phase of STEMI...
November 7, 2022: Journal of Clinical Medicine
https://read.qxmd.com/read/36284074/evaluation-of-a-novel-moving-threshold-gating-strategy-for-assessment-of-reticulated-platelets-in-dogs-using-the-advia-2120-analyzer
#23
JOURNAL ARTICLE
Anja Kuhn, Kristina Weiler, Graham Gibbs, Gottfried Prechtl, Natali Bauer, Andreas Moritz
BACKGROUND: A novel method using a moving threshold (r-PLTmt) to determine canine r-PLTs (reticulated platelets) has been introduced for ADVIA 2120 software v6.11.7. OBJECTIVES: We aimed to evaluate absolute (ar-PLTmt) and percent (%r-PLTmt) prior to and after visual inspection of scattergrams (ar-PLTmtv, %rPLTmtv) compared with flow cytometry (flow) and to determine reference intervals (RIs) in 120 dogs. METHODS: For method comparison, 42 blood samples of healthy and thrombocytopenic dogs were included...
October 25, 2022: Veterinary Clinical Pathology
https://read.qxmd.com/read/36272416/neutrophil-plucking-on-megakaryocytes-drives-platelet-production-and-boosts-cardiovascular-disease
#24
JOURNAL ARTICLE
Tobias Petzold, Zhe Zhang, Iván Ballesteros, Inas Saleh, Amin Polzin, Manuela Thienel, Lulu Liu, Qurrat Ul Ain, Vincent Ehreiser, Christian Weber, Badr Kilani, Pontus Mertsch, Jeremias Götschke, Sophie Cremer, Wenwen Fu, Michael Lorenz, Hellen Ishikawa-Ankerhold, Elisabeth Raatz, Shaza El-Nemr, Agnes Görlach, Esther Marhuenda, Konstantin Stark, Joachim Pircher, David Stegner, Christian Gieger, Marc Schmidt-Supprian, Florian Gaertner, Isaac Almendros, Malte Kelm, Christian Schulz, Andrés Hidalgo, Steffen Massberg
Intravascular neutrophils and platelets collaborate in maintaining host integrity, but their interaction can also trigger thrombotic complications. We report here that cooperation between neutrophil and platelet lineages extends to the earliest stages of platelet formation by megakaryocytes in the bone marrow. Using intravital microscopy, we show that neutrophils "plucked" intravascular megakaryocyte extensions, termed proplatelets, to control platelet production. Following CXCR4-CXCL12-dependent migration towards perisinusoidal megakaryocytes, plucking neutrophils actively pulled on proplatelets and triggered myosin light chain and extracellular-signal-regulated kinase activation through reactive oxygen species...
October 18, 2022: Immunity
https://read.qxmd.com/read/36122665/platelet-acute-leukemia-interactions
#25
REVIEW
Li Zhang, Jing Liu, Xiang Qin, Wenjun Liu
Acute leukemia (AL) is a hematological malignancy with high morbidity and mortality that is caused by abnormal hematopoietic stem cells. AL can change the parameters, quality, and function of platelets through numerous mechanisms, resulting in bleeding and even death in AL patients. Hence, AL patients are often clinically treated using normal platelet transfusion. However, studies have found that platelets can also affect AL cells. This review discusses the changes occurring in platelet count, mean platelet volume, platelet distribution width, reticulated platelets, platelet membrane glycoprotein, platelet aggregation, and activation in AL patients, the causes of these changes, and the possible significance of these changes for patient prognosis...
September 16, 2022: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36090760/chemically-modified-in-vitro-transcribed-mrna-encoding-thrombopoietin-stimulates-thrombopoiesis-in-mice
#26
JOURNAL ARTICLE
Yu Zhang, Xiaodong Xi, Hang Yu, Liuyan Yang, Jinzhong Lin, Wen Yang, Junling Liu, Xuemei Fan, Yingjie Xu
The use of messenger RNA (mRNA) enables the transient production of therapeutic proteins with stable and predictable translational kinetics and without the risk of insertional mutagenesis. Recent findings highlight the enormous potential of mRNA-based therapeutics. Here, we describe the synthesis of chemically modified thrombopoietin (TPO) mRNA through in vitro transcription and in vivo delivery via lipid nanoparticles (LNPs). After delivery of TPO mRNA in mice, compared with normal physiological values, plasma TPO protein levels increased over 1000-fold in a dose-dependent manner...
September 13, 2022: Molecular Therapy. Nucleic Acids
https://read.qxmd.com/read/36079028/platelet-derived-procoagulant-microvesicles-are-elevated-in-patients-with-retinal-vein-occlusion-rvo
#27
JOURNAL ARTICLE
Adrianna Marcinkowska, Nina Wolska, Boguslawa Luzak, Slawomir Cisiecki, Karol Marcinkowski, Marcin Rozalski
The etiopathogenesis of retinal vein occlusion (RVO) is multifactorial, and the contribution of platelets to RVO development has not been fully elucidated. We aimed to analyze platelet function in RVO patients ( n = 35) and controls ( n = 35). We found a higher ( p < 0.05) level of soluble P-selectin in RVO group vs. controls. Additionally, in RVO patients, the concentration of platelet-derived microvesicles was higher ( p < 0.05), and the difference between groups was deeper for the fraction of platelet-derived microvesicles with the procoagulant phenotype ( p < 0...
August 30, 2022: Journal of Clinical Medicine
https://read.qxmd.com/read/36037520/temporal-in-vivo-platelet-labelling-in-mice-reveals-age-dependent-receptor-expression-and-conservation-of-specific-mrnas
#28
JOURNAL ARTICLE
Paul C Armstrong, Harriet E Allan, Nicholas S Kirkby, Clemens Gutmann, Abhishek Joshi, Marilena Crescente, Jane A Mitchell, Manuel Mayr, Timothy D Warner
The proportion of young platelets, also known as newly formed or reticulated, within the overall platelet population has been clinically correlated with adverse cardiovascular outcomes. Our understanding of this is incomplete, however, because of limitations in the technical approaches available to study platelets of different ages. In this study we have developed and validated an in vivo 'temporal labelling' approach using injectable fluorescent anti-platelet antibodies to sub-divide platelets by age and assess differences in functional and molecular characteristics...
August 29, 2022: Blood Advances
https://read.qxmd.com/read/35965320/reticulation-pattern-without-honeycombing-on-high-resolution-ct-is-associated-with-the-risk-of-disease-progression-in-interstitial-lung-diseases
#29
JOURNAL ARTICLE
Minna Mononen, Eeva Saari, Hannele Hasala, Hannu-Pekka Kettunen, Sanna Suoranta, Hanna Nurmi, Miia Kärkkäinen, Tuomas Selander, Jukka Randell, Jari Laurikka, Toomas Uibu, Heikki Koskela, Riitta Kaarteenaho, Minna Purokivi
BACKGROUND: The disease course of idiopathic pulmonary fibrosis (IPF) is progressive and occasionally, other types of interstitial lung disease (ILD) may progress similarly to IPF. This study aimed to evaluate risk factors for disease progression within 24 months in patients with various ILDs. METHODS: This prospective study obtained 97 patients with a suspected ILD who underwent a transbronchial lung cryobiopsy. The extent of several high-resolution computed tomography (HRCT) patterns was assessed...
August 14, 2022: BMC Pulmonary Medicine
https://read.qxmd.com/read/35945713/comparing-the-efficacy-of-different-intra-articular-injections-for-knee-osteoarthritis-a-network-analysis
#30
JOURNAL ARTICLE
Xingzhen Lin, Fang Zhi, Qin Lan, Weixiong Deng, Xinju Hou, Qing Wan
BACKGROUND: The findings on the effectiveness of platelet-rich plasma, ozone, and hyaluronic acid in the treatment of osteoarthritis of the knee are controversial, and the existing original studies and meta-analyses are mostly comparisons of a single joint cavity injection method, lacking direct and indirect comparisons of different drugs in the joint cavity. The lack of direct and indirect comparisons of different drugs in the joint cavity makes it difficult to have a clearer and more comprehensive understanding of joint cavity injection methods...
August 5, 2022: Medicine (Baltimore)
https://read.qxmd.com/read/35898487/a-net-meta-analysis-of-the-effectiveness-of-different-types-of-dressings-in-the-treatment-of-diabetic-foot
#31
JOURNAL ARTICLE
Ying Wang, Nan Chao, Doudou Yin
Objective: This is an analysis of the impact of a new dressing commonly used to treat diabetic foot (DFU). Methods: Chinese and English databases were searched to collect clinical randomized controlled studies (RCTs) of various types of dressings for the treatment of DFU, and the healing rates of the different dressings were combined by reticulation meta-analysis. Results: The aggregate of the 36 RCTs included in this study analysed the healing rates of nine dressings: conventional dressing, alginate dressing, chitosan dressing, hyaluronic acid dressing, platelet-rich plasma dressing, amniotic membrane dressing, honey dressing, human recombinant growth factor dressing, and silver ionomer dressing...
2022: Computational and Mathematical Methods in Medicine
https://read.qxmd.com/read/35463762/platelet-subtypes-in-inflammatory-settings
#32
REVIEW
Muataz Ali Hamad, Krystin Krauel, Nancy Schanze, Nadine Gauchel, Peter Stachon, Thomas Nuehrenberg, Mark Zurek, Daniel Duerschmied
In addition to their essential role in hemostasis and thrombosis, platelets also modulate inflammatory reactions and immune responses. This is achieved by specialized surface receptors as well as secretory products including inflammatory mediators and cytokines. Platelets can support and facilitate the recruitment of leukocytes into inflamed tissue. The various properties of platelet function make it less surprising that circulating platelets are different within one individual. Platelets have different physical properties leading to distinct subtypes of platelets based either on their function (procoagulant, aggregatory, secretory) or their age (reticulated/immature, non-reticulated/mature)...
2022: Frontiers in Cardiovascular Medicine
https://read.qxmd.com/read/35415158/tissue-engineered-successful-reconstruction-of-a-complex-traumatized-lower-extremity
#33
Srinjoy Saha
Introduction: Tissue engineered reconstruction is a minimally invasive approach for healing major complex wounds successfully. It combines accurate, conservative debridement with a specially adapted suction method, platelet-rich plasma (PRP) injections, and biomaterial application to salvage injured tissues and grows new soft tissues over wounds. Case Presentation: A healthy young man in his early 30s presented to our emergency department with complex knee-thigh injuries following a high-velocity automobile accident...
September 2021: Journal of Orthopaedic Case Reports
https://read.qxmd.com/read/35321562/role-of-reticulated-platelets-in-cardiovascular-disease
#34
REVIEW
Dario Bongiovanni, Jiaying Han, Melissa Klug, Kilian Kirmes, Giacomo Viggiani, Moritz von Scheidt, Nina Schreiner, Gianluigi Condorelli, Karl-Ludwig Laugwitz, Isabell Bernlochner
Human platelets differ considerably with regard to their size, RNA content and thrombogenicity. Reticulated platelets (RPs) are young, hyper-reactive platelets that are newly released from the bone marrow. They are larger and contain more RNA compared to older platelets. In comparison to more mature platelets, they exhibit a significantly higher thrombogenicity and are known to be elevated in patients with an increased platelet turnover such as, diabetics and after acute myocardial infarction. Several studies have shown that RPs correlate with an insufficient antiplatelet response to aspirin and specific P2Y12 receptor inhibitors...
May 2022: Arteriosclerosis, Thrombosis, and Vascular Biology
https://read.qxmd.com/read/35085240/impact-of-high-platelet-turnover-on-the-platelet-transcriptome-results-from-platelet-rna-sequencing-in-patients-with-sepsis
#35
JOURNAL ARTICLE
Thomas G Nührenberg, Jasmin Stöckle, Federico Marini, Mark Zurek, Björn A Grüning, Vladimir Benes, Lutz Hein, Franz-Josef Neumann, Christian Stratz, Marco Cederqvist, Willibald Hochholzer
BACKGROUND: Sepsis is associated with high platelet turnover and elevated levels of immature platelets. Changes in the platelet transcriptome and the specific impact of immature platelets on the platelet transcriptome remain unclear. Thus, this study sought to address whether and how elevated levels of immature platelets affect the platelet transcriptome in patients with sepsis. METHODS: Blood samples were obtained from patients with sepsis requiring vasopressor therapy (n = 8) and from a control group of patients with stable coronary artery disease and otherwise similar demographic characteristics (n = 8)...
2022: PloS One
https://read.qxmd.com/read/35042240/analysis-of-preplatelets-and-their-barbell-platelet-derivatives-by-imaging-flow-cytometry
#36
JOURNAL ARTICLE
Samuel Kemble, Amanda Dalby, Gillian C Lowe, Phillip L R Nicolson, Steve P Watson, Yotis Senis, Steven G Thomas, Paul Harrison
Circulating large "preplatelets" undergo fission via barbell platelet intermediates into two smaller, mature platelets. In this study, we determine whether preplatelets and/or barbells are equivalent to reticulated/immature platelets by using ImageStream flow cytometry and super-resolution microscopy. Immature platelets, preplatelets, and barbells were quantified in healthy and thrombocytopenic mice, healthy human volunteers, and patients with immune thrombocytopenia or undergoing chemotherapy. Preplatelets and barbells were 1...
May 10, 2022: Blood Advances
https://read.qxmd.com/read/35037230/autoimmune-mediated-thrombocytopenia-after-allogeneic-hematopoietic-stem-cell-transplantation-significance-of-detecting-reticulated-platelets-and-glycoprotein-specific-platelet-autoantibodies
#37
JOURNAL ARTICLE
Keigo Akuta, Kentaro Fukushima, Keiichi Nakata, Satoru Hayashi, Jun Toda, Yasuhiro Shingai, Kazuhito Tsutsumi, Tomohisa Machida, Akihisa Hino, Shinsuke Kusakabe, Yukiko Doi, Jiro Fujita, Hisashi Kato, Tetsuo Maeda, Takafumi Yokota, Yoshiaki Tomiyama, Naoki Hosen, Hirokazu Kashiwagi
Autoimmune hematological disorders are rare complications after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Diagnosis of immune thrombocytopenia (ITP) is challenging, especially after allo-HSCT, because various complications such as graft-versus-host disease, disease relapse, viral infection, thrombotic microangiopathy, and drug side effects can also cause thrombocytopenia. Assessment of reticulated platelets (RP) and plasma thrombopoietin (TPO) levels may be useful to distinguish between ITP and hypoplastic thrombocytopenia...
March 2022: International Journal of Hematology
https://read.qxmd.com/read/34602784/immature-platelet-fraction-its-clinical-utility-in-thrombocytopenia-patients
#38
JOURNAL ARTICLE
Garima Goel, Shruti Semwal, Akriti Khare, Deepti Joshi, Chaitanya K Amerneni, Abhijit Pakhare, Neelkamal Kapoor
Objectives Etiology of thrombocytopenia is multifactorial and its pathogenesis should be distinguished for appropriate management. Newly formed immature platelets are called reticulated platelets (RPs) and can be estimated in peripheral blood using automated hematology analyzers, which express them as immature platelet fraction (IPF). In the present study we intend to assess and establish the clinical utility of IPF in differentiating the two major causes of thrombocytopenia-decreased production and increased destruction of platelets-along with determining its significance in monitoring patients with thrombocytopenia...
September 2021: Journal of Laboratory Physicians
https://read.qxmd.com/read/34519015/immature-platelets-in-patients-with-covid-19-association-with-disease-severity
#39
JOURNAL ARTICLE
Amir Cohen, Emanuel Harari, Ella Yahud, Michal Cipok, Gabriel Bryk, Nili Karp Lador, Tal Mann, Ami Mayo, Eli I Lev
Coronavirus disease 2019 (Covid-19) is associated with a high incidence of venous and arterial thromboembolic events. Currently, there are no clinical or laboratory markers that predict thrombotic risk. Circulating immature platelets are hyper-reactive platelets, which are associated with arterial thrombotic events. The aim of this study was to assess whether the proportion of circulating immature platelets is associated with disease severity in Covid-19 patients. Patients admitted with Covid-19 disease were prospectively assessed...
October 2021: Journal of Thrombosis and Thrombolysis
https://read.qxmd.com/read/34478523/a-novel-mechanism-of-thrombocytopenia-by-ps-exposure-through-tmem16f-in-sphingomyelin-synthase-1-deficiency
#40
JOURNAL ARTICLE
Yoritaka Fujii, Makoto Taniguchi, Shingo Nagaya, Yoshibumi Ueda, Chieko Hashizume, Ken Watanabe, Hiroyuki Takeya, Takeo Kosaka, Toshiro Okazaki
Sphingomyelin synthase 1 (SMS1) contributes to the generation of membrane sphingomyelin (SM) and affects SM-mediated physiological functions. Here, we describe the hematologic phenotypes, such as reduced circulating platelets and dysfunctional hemostasis, in SMS1-deficient (SMS1-KO) mice. SMS1-KO mice display pathologic manifestations related to idiopathic thrombocytopenia (ITP), including relatively high amounts of peripheral blood reticulated platelets, enhanced megakaryopoiesis in the bone marrow and spleen, and splenomegaly...
October 26, 2021: Blood Advances
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