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Kinji Ohno, Mohammad Alinoor Rahman, Mohammad Nazim, Farhana Nasrin, Yingni Lin, Jun-Ichi Takeda, Akio Masuda
We humans have evolved by acquiring diversity of alternative RNA metabolisms including alternative means of splicing and transcribing non-coding genes, and not by acquiring new coding genes. Tissue-specific and developmental stage-specific alternative RNA splicing is achieved by tightly regulated spatiotemporal regulation of expressions and activations of RNA-binding proteins that recognize their cognate splicing cis-elements on nascent RNA transcripts. Genes expressed at the neuromuscular junction (NMJ) are also alternatively spliced...
January 10, 2017: Journal of Neurochemistry
Dan Li, Xuefeng Liu, Jian Zhou, Jie Hu, Dongdong Zhang, Jing Liu, Yanyan Qiao, Qimin Zhan
: Dysregulated expressions of lncRNAs have been reported in many types of cancers, indicating that they may play critical roles in tumorigenesis. HULC (Highly Up-regulated in Liver Cancer) was firstly characterized in hepatocelluar carcinoma (HCC). However, the detailed mechanisms of HULC remain unclear. Here, we demonstrated a novel mechanism by which lncRNA plays oncogenic roles through modulating the phosphorylation status of its interaction protein. First, we validated that the remarkably increased expression levels of HULC in HCC tissues compared to their adjacent non-cancerous tissues...
December 27, 2016: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Steffi Gieseler-Halbach, Stefan Meltendorf, Mandy Pierau, Soenke Weinert, Florian H Heidel, Thomas Fischer, Juliane Handschuh, Ruediger C Braun-Dullaeus, Martin Schrappe, Jonathan A Lindquist, Peter R Mertens, Ulrich Thomas, Monika C Brunner-Weinzierl
Deregulated proliferation is key to tumor progression. Although unrestricted proliferation of solid tumor cells correlates with the cold-shock protein Y-box (YB)-binding protein-1 accumulation in the nuclei, little is known about its expression and function in hematopoietic malignancies, such as T-cell acute lymphoblastic leukemia (T-ALL). Here we show that YB-1 protein is highly enriched in the nuclei of activated T cells and malignant human T-ALL cell lines but not in resting T cells. YB-1 S(102) mutations that either mimic (S102D) or prevent phosphorylation (S102N) led to accumulation of YB-1 in the nucleus of T cells or strictly excluded it, respectively...
December 23, 2016: Cell Death and Differentiation
Cristina Pagano, Orsola di Martino, Gennaro Ruggiero, Andrea Maria Guarino, Nathalie Mueller, Rima Siauciunaite, Markus Reischl, Nicholas Simon Foulkes, Daniela Vallone, Viola Calabrò
Correct spatial and temporal control of cell proliferation is of fundamental importance for tissue homeostasis. Its deregulation has been associated with several pathological conditions. In common with almost every aspect of plant and animal biology, cell proliferation is dominated by day-night rhythms generated by the circadian clock. However, our understanding of the crosstalk between the core clock and cell cycle control mechanisms remains incomplete. In this study, using zebrafish as a vertebrate model system, we show that the nuclear localization of the Y-box binding protein 1 (YB-1), a regulator of cyclin expression and a hallmark of certain cancers, is robustly regulated by the circadian clock...
December 20, 2016: Oncotarget
Jinjing Jia, Yan Zheng, Wei Wang, Yongping Shao, Zhengxiao Li, Qiong Wang, Yuan Wang, Huling Yan
The cathelicidin antimicrobial peptide, LL-37, is a multifunctional peptide with a broad spectrum of antimicrobial activities, such as chemotaxis and neutralizing endotoxins. Previous studies have demonstrated that it LL‑37 serves a functional role in the development of numerous types of cancer including ovarian, breast, prostate and lung cancer. However, its role in the development of malignant melanoma (MM) remains unclear. To determine the role of LL‑37 and the potential interaction with Y-box binding protein 1 (YB‑1) in MM, RNA interference, western blot, reverse transcription-quantitative polymerase chain reaction, MTT and Transwell assays were performed...
January 2017: Molecular Medicine Reports
Hsiao-Mei Chao, Hong-Xuan Huang, Po-Hsiang Chang, Kuo-Chang Tseng, Atsushi Miyajima, Edward Chern
Y-box binding protein-1 (YB-1) is a pleiotropic molecule that binds DNA to regulate genes on a transcriptional level in the nucleus and binds RNA to modulate gene translation in the cytoplasm. In our previous studies, YB-1 was also characterized as a fetal hepatic protein that regulates the maturation of hepatocytes and is upregulated during liver regeneration. Moreover, YB-1 has been shown to be expressed in human hepatocellular carcinoma (HCC). However, the role of YB-1 in HCC remains unclear. Here, we aimed to characterize the role of YB-1 in HCC...
1, 2016: Oncotarget
Qiudi Geng, Besa Xhabija, Colleen Knuckle, Christopher A Bonham, Panayiotis O Vacratsis
Human YVH1 (hYVH1), also known as dual specificity phosphatase 12 (DUSP12) is a poorly characterized atypical dual specificity phosphatase widely conserved throughout evolution. Recent findings have demonstrated that hYVH1 expression affects cellular DNA content and is a novel cell survival phosphatase preventing both thermal and oxidative stress induced cell death, while studies in yeast have established YVH1 as a novel 60S ribosome biogenesis factor. In this study, we have isolated novel hYVH1 associating proteins from human U2OS osteosarcoma cells using affinity chromatography coupled to mass spectrometry employing ion mobility separation...
November 17, 2016: Journal of Biological Chemistry
Daria A Mordovkina, Ekaterina R Kim, Ilya A Buldakov, Alexey V Sorokin, Irina A Eliseeva, Dmitry N Lyabin, Lev P Ovchinnikov
The DNA/RNA-binding protein YB-1 (Y-box binding protein 1) performs multiple functions both in the cytoplasm and the nucleus of the cell. Generally localized to the cytoplasm, under certain conditions YB-1 is translocated to the nucleus. Here we report for the first time a transport factor that mediates YB-1 nuclear import - transportin-1. The YB-1/transportin-1 complex can be isolated from HeLa cell extract. Nuclear import of YB-1 and its truncated form YB-1 (1-219) in in vitro transport assay was diminished in the presence of a competitor substrate and ceased in the presence of transportin-1 inhibitor M9M...
November 25, 2016: Biochemical and Biophysical Research Communications
Qingqing Liu, Tao Tao, Fang Liu, Runzhou Ni, Cuihua Lu, Aiguo Shen
As an essential post-translational modification, O-GlcNAcylation has been thought to be able to modulate various nuclear and cytoplasmic proteins and is emerging as a key regulator of multiple biological processes, such as transcription, cell growth, signal transduction, and cell motility. Recently, authoritative glycomics analyses have reported extensive crosstalk between O-GlcNAcylation and phosphorylation, which always dynamically interplay with each other and regulate signaling, transcription, and other cellular processes...
October 14, 2016: Experimental Cell Research
Yunwei Ou, Zitong Zhao, Weimin Zhang, Qingnan Wu, Chuanyue Wu, Xuefeng Liu, Ming Fu, Nan Ji, Dan Wang, Jiaji Qiu, Liwei Zhang, Chunjiang Yu, Yongmei Song, Qimin Zhan
Kindlin-2 promotes carcinogenesis through regulation of cell-cell and cell-extracellular matrix adhesion. However, the role of Kindlin-2 in glioma has not been elucidated. We investigated Kindlin-2 expression in 188 human glioma tissue samples. High Kindlin-2 expression was correlated with high pathological grade and a worse prognosis. Kindlin-2 promoted glioma cell motility and proliferation both in vitro and in vivo. Importantly, Kindlin-2 activated the EGFR pathway and increased EGFR mRNA levels. In addition to up-regulating Y-box binding protein-1 (YB-1) and β-catenin expression, Kindlin-2 formed a transcriptional complex with YB-1 and β-catenin that bound to the EGFR promoter and enhanced transcription...
October 4, 2016: Oncotarget
Kenjiro Imada, Masaki Shiota, Kentaro Kuroiwa, Masaaki Sugimoto, Tatsuro Abe, Kenichi Kohashi, Akira Yokomizo, Masatoshi Eto, Seiji Naito, Yoshinao Oda
BACKGROUND: FOXO3a is a member of the forkhead O transcription factors. FOXO3a induces the factors that contribute to cell cycle arrest and is considered a tumor suppressor in several malignant tumors. Y-box binding protein-1 (YB-1) is a multifunctional protein whose high expression is correlated with poor prognoses in various malignant tumors. In the current study, we investigated the relationship between FOXO3a and YB-1 to validate their functional roles in prostate cancer. METHODS: Western blotting and cytotoxicity assays were conducted in prostate cancer cells, LNCaP, and 22Rv1 cells...
February 2017: Prostate
Xue Wang, Bing Sun, Christelle Mbondji, Santanu Biswas, Jiangqin Zhao, Indira Hewlett
Macrophages contribute to HIV-1 pathogenesis by forming a viral reservoir that serve as a viral source for the infection of CD4 T cells. The relationship between HIV-1 latent infection and superinfection in macrophages has not been well studied. Using susceptible U1 cells chronically infected with HIV-1, we studied the effects of HIV superinfection on latency and differences in superinfection with HIV-1 and HIV-2 in macrophages. We found that HIV-1 (MN) superinfection displayed increased HIV-1 replication in a time-dependent manner; while cells infected with HIV-2 (Rod) initially showed increased HIV-1 replication, followed by a decrease in HIV-1 RNA production...
September 23, 2016: Journal of Cellular Physiology
Guangchao Zhang, Baojia Deng, Shaowu Wang, Yun Wei, Shuangliu Zhou, Xiancui Zhu, Zeming Huang, Xiaolong Mu
Different di and trinuclear rare-earth metal complexes supported by 3-amido appended indolyl ligands were synthesized and their catalytic activities towards isoprene polymerization were investigated. Treatment of [RE(CH2SiMe3)3(thf)2] with 1 equiv. of 3-(CyN[double bond, length as m-dash]CH)C8H5NH in toluene or in THF afforded dinuclear rare-earth metal alkyl complexes having indolyl ligands in different hapticities with central metals {[η(2):η(1)-μ-η(1)-3-(CyNCH(CH2SiMe3))Ind]RE-(thf)(CH2SiMe3)}2 (Cy = cyclohexyl, Ind = Indolyl, RE = Yb (1), Er (2), Y (3)) or {[η(1)-μ-η(1)-3-(CyNCH(CH2SiMe3))Ind]RE-(thf)2(CH2SiMe3)}2 (RE = Yb (4), Er (5), Y (6), Gd (7)), respectively...
October 21, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
Jialin Wang, Lydia Gibbert, Sonja Djudjaj, Christina Alidousty, Thomas Rauen, Uta Kunter, Andreas Rembiak, Dieter Enders, Vera Jankowski, Gerald S Braun, Jürgen Floege, Tammo Ostendorf, Ute Raffetseder
Virtually all chronic kidney diseases progress towards tubulointerstitial fibrosis. In vitro, Y-box protein-1 (YB-1) acts as a central regulator of gene transcription and translation of several fibrosis-related genes. However, it remains to be determined whether its pro- or antifibrotic propensities prevail in disease. Therefore, we investigated the outcome of mice with half-maximal YB-1 expression in a model of renal fibrosis induced by unilateral ureteral obstruction. Yb1(+/-) animals displayed markedly reduced tubular injury, immune cell infiltration and renal fibrosis following ureteral obstruction...
December 2016: Kidney International
Toru Tanaka, Sachiyo Ohashi, Shunsuke Kobayashi
In cancer cells, anticancer reagents often trigger nuclear accumulation of YB-1, which participates in the progression of cancer malignancy. YB-1 has a non-canonical nuclear localization signal (YB-NLS). Here we found that four nucleocytoplasmic-shuttling RNA-binding proteins and p53 interact specifically with the YB-NLS and co-accumulate with YB-1 in the nucleus of actinomycin D-treated cells. To elucidate the roles of these YB-NLS-binding proteins, we performed a dominant-negative experiment in which a large excess of YB-NLS interacts with the YB-NLS-binding proteins, and showed inhibitory effects on actinomycin D-induced nuclear transport of endogenous YB-1 and subsequent MDR1 gene expression...
September 23, 2016: Biochemical and Biophysical Research Communications
Elizaveta E Alemasova, Nina A Moor, Konstantin N Naumenko, Mikhail M Kutuzov, Maria V Sukhanova, Pavel E Pestryakov, Olga I Lavrik
Base excision repair (BER) is a flagship DNA repair system responsible for maintaining genome integrity. Apart from basal enzymes, this system involves several accessory factors essential for coordination and regulation of DNA processing during substrate channeling. Y-box-binding protein 1 (YB-1), a multifunctional factor that can interact with DNA, RNA, poly(ADP-ribose) and plenty of proteins including DNA repair enzymes, is increasingly considered as a non-canonical protein of BER. Here we provide quantitative characterization of YB-1 physical interactions with key BER factors such as PARP1, PARP2, APE1, NEIL1 and pol β and comparison of the full-length YB-1 and its C-terminally truncated nuclear form in regard to their binding affinities for BER proteins...
December 2016: Biochimica et Biophysica Acta
Thomas Rauen, Bjoern C Frye, Jialin Wang, Ute Raffetseder, Christina Alidousty, Abdelaziz En-Nia, Jürgen Floege, Peter R Mertens
Hypoxia-dependent gene regulation is largely orchestrated by hypoxia-inducible factors (HIFs), which associate with defined nucleotide sequences of hypoxia-responsive elements (HREs). Comparison of the regulatory HRE within the 3' enhancer of the human erythropoietin (EPO) gene with known binding motifs for cold shock protein Y-box (YB) protein-1 yielded strong similarities within the Y-box element and 3' adjacent sequences. DNA binding assays confirmed YB-1 binding to both, single- and double-stranded HRE templates...
September 16, 2016: Biochemical and Biophysical Research Communications
Juan-Ramón Jiménez, Ismael F Díaz-Ortega, Eliseo Ruiz, Daniel Aravena, Simon J A Pope, Enrique Colacio, Juan Manuel Herrera
Three new sets of mononuclear Ln(III) complexes of general formulas [LnL3 ]⋅CH3 OH [Ln(III) =Yb (1), Er (2), Dy (3), Gd (4), and Eu (5)], [LnL2 (tmh)(CH3 OH)]⋅n H2 O⋅m CH3 OH [Ln(III) =Yb (1 b), Er (2 b), Dy (3 b), Gd (4 b)], and [LnL2 (tta)(CH3 OH)]⋅CH3 OH [Ln(III) =Yb (1 c), Er (2 c), Dy (3 c), Gd (4 c)] were prepared by the reaction of Ln(CF3 SO3 )⋅n H2 O salts with the tridentate ligand 2-(tetrazol-5-yl)-1,10-phenanthroline (HL) and, for the last two sets, additionally with the β-diketonate ligands 2,2,6,6-tetramethylheptanoate (tmh) and 2-thenoyltrifluoroacetonate (tta), respectively...
October 4, 2016: Chemistry: a European Journal
Xiaobing Miao, Yaxun Wu, Yuchan Wang, Xinghua Zhu, Haibing Yin, Yunhua He, Chunsun Li, Yushan Liu, Xiaoyun Lu, Yali Chen, Rong Shen, Xiaohong Xu, Song He
YB-1 is a multifunctional protein, which has been shown to correlate with resistance to treatment of various tumor types. This study investigated the expression and biologic function of YB-1 in diffuse large B-cell lymphoma (DLBCL). Immunohistochemical analysis showed that the expression statuses of YB-1 and pYB-1(S102) were reversely correlated with the clinical outcomes of DLBCL patients. In addition, we found that YB-1 could promote the proliferation of DLBCL cells by accelerating the G1/S transition. Ectopic expression of YB-1 could markedly increase the expression of cell cycle regulators cyclin D1 and cyclin E...
August 15, 2016: Experimental Cell Research
Jui-Wen Ma, Chao-Ming Hung, Ying-Chao Lin, Chi-Tang Ho, Jung-Yie Kao, Tzong-Der Way
Human epidermal growth factor receptor-2 (HER-2)-positive breast cancer tends to be aggressive, highly metastatic, and drug resistant and spreads rapidly. Studies have indicated that emodin inhibits HER-2 expression. This study compared the HER-2-inhibitory effects of two compounds extracted from rhubarb roots: aloe-emodin (AE) and rhein. Our results indicated that AE exerted the most potent inhibitory effect on HER-2 expression. Treatment of HER-2-overexpressing breast cancer cells with AE reduced tumor initiation, cell migration, and cell invasion...
September 13, 2016: Oncotarget
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