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https://www.readbyqxmd.com/read/28319996/oral-direct-acting-agent-therapy-for-hepatitis-c-virus-infection-a-systematic-review
#1
Oluwaseun Falade-Nwulia, Catalina Suarez-Cuervo, David R Nelson, Michael W Fried, Jodi B Segal, Mark S Sulkowski
Background: Rapid improvements in hepatitis C virus (HCV) therapy have led to the approval of multiple oral direct-acting antiviral (DAA) regimens by the U.S. Food and Drug Administration (FDA) for treatment of chronic HCV infection. Purpose: To summarize published literature on the efficacy and safety of oral DAAs for treatment of persons with chronic HCV infection. Data Sources: MEDLINE and EMBASE from inception through 1 November 2016. Study Selection: 42 English-language studies from controlled and single-group registered clinical trials of adults with HCV infection that evaluated at least 8 weeks of an FDA-approved interferon-free HCV regimen that included at least 2 DAAs...
March 21, 2017: Annals of Internal Medicine
https://www.readbyqxmd.com/read/28317313/profiling-of-antioxidative-enzyme-expression-induced-by-various-food-components-using-targeted-proteome-analysis
#2
Sven Bartenbacher, Christiane Östreicher, Monika Pischetsrieder
SCOPE: A method was developed for targeted proteome analysis of the expression profile of a set of antioxidative enzymes in rat macrophages and applied to screen the antioxidative potential of several food components/foods. METHODS AND RESULTS: Expression profiles of heme oxygenase 1, peroxiredoxin 1, thioredoxin reductase 1, glutathione reductase, glutathione-S transferase P1, and superoxide dismutase 1 were analyzed by nanoLC-MS/MS in selected scheduled reaction monitoring (sSRM) mode monitoring 2-3 peptides per protein and 3 transitions per peptide...
March 20, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28316108/cry1a-b-16-toxin-from-bacillus-thuringiensis-theoretical-refinement-of-three-dimensional-structure-and-prediction-of-peptides-as-molecular-markers-for-detection-of-genetically-modified-organisms
#3
Alexandra Plácido, Andreia Coelho, Lucas Abreu do Nascimento, Andreanne Gomes Vasconcelos, Maria Fátima Barroso, Joilson Ramos-Jesus, Vladimir Costa, Francisco das Chagas Alves Lima, Cristina Delerue-Matos, Ricardo Martins Ramos, Mariela M Marani, José Roberto de Souza de Almeida Leite
Transgenic maize produced by the insertion of the Cry transgene into its genome became the second most cultivated crop worldwide. Cry gene from Bacillus thuringiensis kurstaki expresses protein derivatives of crystalline endotoxins which confer insect resistance onto the maize crop. Mandatory labeling of processed food containing or made by genetically modified organisms is in force in many countries, so, it is very urgent to develop fast and practical methods for GMO identification, e.g., biosensors. In the absence of an available empirical structure of Cry1A(b)16 protein, a theoretical model was effectively generated, in this work, by homology modeling and molecular dynamics simulations based on two available homologous protein structures...
March 18, 2017: Proteins
https://www.readbyqxmd.com/read/28315798/bone-morphogenetic-proteins-in-anterior-cervical-fusion-a-systematic-review-and-meta-analysis
#4
REVIEW
Shayan Abdollah Zadegan, Aidin Abedi, Seyed Behnam Jazayeri, Hirbod Nasiri Bonaki, Seyed Behzad Jazayeri, Alexander R Vaccaro, Vafa Rahimi-Movaghar
OBJECTIVE: Bone morphogenetic proteins (BMPs) have been commonly used as a graft substitute in spinal fusion. Although the Food and Drug Administration (FDA) issued warning on life-threatening complications of recombinant human BMPs (rhBMPs) in cervical spine fusion in 2008, their off-label use has been continued. This investigation aimed to review the evidence for the use of rhBMP-2 and rhBMP-7 in anterior cervical spine fusions. METHODS: A comprehensive search was performed through Ovid (MEDLINE), PubMed and Embase...
March 15, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28315780/simplifying-mental-math-changing-how-added-sugars-are-displayed-on-the-nutrition-facts-label-can-improve-consumer-understanding
#5
Neha Khandpur, Dan J Graham, Christina A Roberto
BACKGROUND: Proposed variations to Nutrition Facts Labels (NFL) have included the display of added sugars (AS) content, but its impact on consumer understanding is poorly understood. OBJECTIVE: To examine the degree to which different formats for displaying AS influence consumer understanding, perceptions, and purchase intentions. DESIGN: Randomized-controlled online experiment. PARTICIPANTS: A sample of 2509 U.S adults...
March 15, 2017: Appetite
https://www.readbyqxmd.com/read/28315226/recent-advances-in-the-detection-of-allergens-in-foods
#6
Silvia de la Cruz, Inés López-Calleja, Rosario Martín, Isabel González, Marcos Alcocer, Teresa García
Food allergy is a public health issue that has significantly increased worldwide in the past decade affecting consumers' quality of life and making increasing demands on health service resources. Despite recent advances in many areas of diagnosis and treatment, our general knowledge of the basic mechanisms of the disease remained limited, i.e., not at pace with the exponential number of new cases and the explosion of the new technologies. For sensitized individuals, the only effective way to prevent allergic reactions is the strict avoidance of the offending food...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28315214/detection-of-food-allergens-by-taqman-real-time-pcr-methodology
#7
Aina García, Raquel Madrid, Teresa García, Rosario Martín, Isabel González
Real-time PCR (polymerase chain reaction) has shown to be a very effective technology for the detection of food allergens. The protocol described herein consists on a real-time PCR assay targeting the plant ITS (Internal Transcribed Spacer) region, using species-specific primers and hydrolysis probes (Taqman) dual labeled with a reporter fluorophore at the 5' end (6-carboxyfluorescein, FAM) and a quencher fluorophore at the 3' end (Blackberry, BBQ). The species-specific real-time PCR systems (primers/probe) described in this work allowed the detection of different nuts (peanut, hazelnut, pistachio, almond, cashew, macadamia, walnut and pecan), common allergens present in commercial food products, with a detection limit of 0...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28304196/a-limited-survey-of-dark-chocolate-bars-obtained-in-the-united-states-for-undeclared-milk-and-peanut-allergens
#8
Binaifer Bedford, Ye Yu, Xue Wang, Eric A E Garber, Lauren S Jackson
Undeclared allergens in chocolate products have been responsible for numerous allergen-related recalls in the United States. A survey was conducted to determine the prevalence of undeclared milk and peanut in 88 and 78 dark chocolate bars, respectively. Concentrations of milk (as nonfat dry milk) or peanut in three samples of each chocolate product were determined with two milk- or peanut-specific enzyme-linked immunosorbent assay kits. In 75% of the chocolate bar products with a milk advisory statement, milk concentrations were above the limit of quantitation (2...
March 17, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28301098/pharmacokinetics-of-a-novel-anagrelide-extended-release-formulation-in-healthy-subjects-food-intake-and-comparison-with-a-reference-product
#9
Petro E Petrides, Christian Schoergenhofer, Rudolf Widmann, Bernd Jilma, Christoph S Klade
Anagrelide is an established therapy for essential thrombocythemia. Common adverse effects have been linked to peak plasma concentrations of anagrelide and its 3OH metabolite. Our study was performed to investigate the pharmacokinetics (PK) of a novel anagrelide extended-release (AER) formulation and its active metabolites. Thirty healthy volunteers were randomized to receive either 2 mg AER (under fasting and fed conditions) or 2 mg commercially available reference product (CARP) in an open-label, 3-way crossover trial with washout periods of 6 days...
March 16, 2017: Clinical Pharmacology in Drug Development
https://www.readbyqxmd.com/read/28299693/piezoelectric-cantilever-biosensors-for-label-free-real-time-detection-of-dna-and-rna
#10
Alexander P Haring, Ellen Cesewski, Blake N Johnson
This chapter reviews the design, fabrication, characterization, and application of piezoelectric-excited millimeter-sized cantilever (PEMC) sensors. The sensor transduction mechanism, sensing principle, and mode of operation are discussed. Bio-recognition strategies and surface functionalization methods for detection of DNA and RNA are discussed with a focus on self-assembly-based approaches. Methods for the verification of biosensor response via secondary binding assays, reversible binding assays, and the integration of complementary transduction mechanisms are presented...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28296078/botulinum-neurotoxin-type-b-uses-a-distinct-entry-pathway-mediated-by-cdc42-into-intestinal-cells-versus-neuronal-cells
#11
Chloé Connan, Marie Voillequin, Carolina Varela Chavez, Christelle Mazuet, Christian Leveque, Sandrine Vitry, Alain Vandewalle, Michel R Popoff
Botulinum neurotoxins (BoNTs) are responsible for severe flaccid paralysis by inhibiting the release of acetylcholine at the neuromuscular junctions. BoNT/B most often induces mild forms of botulism with predominant dysautonomic symptoms. In food borne botulism and botulism by intestinal colonization such as infant botulism, which are the most frequent naturally acquired forms of botulism, the digestive tract is the main entry route of BoNTs into the organism. We previously showed that BoNT/B translocates through mouse intestinal barrier by an endocytosis-dependent mechanism and subsequently targets neuronal cells, mainly cholinergic neurons, in the intestinal mucosa and musculosa...
March 11, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28295718/the-practice-and-perception-of-precautionary-allergen-labelling-by-the-australasian-food-manufacturing-industry
#12
Giovanni A Zurzolo, Rachel L Peters, Jennifer J Koplin, Maximilian de Courten, Michael L Mathai, Jason A Tye-Din, Mimi L K Tang, Dianne E Campbell, Anne-Louise Ponsonby, Susan L Prescott, Lyle Gurrin, Shyamali Dharmage, Katrina J Allen
BACKGROUND: The precautionary allergen labelling (PAL) and Voluntary Incidental Trace Allergen Labelling (VITAL(®) ) tools were designed by industry to assist consumers with selecting safe foods for consumption. However, a sizeable proportion of food products bear no label and it is unclear whether these products are free from allergens and therefore safe to consume or have simply not undergone a risk assessment and therefore remain unlabelled for that reason. OBJECTIVE: To assess the prevalence of unlabelled products that have undergone a risk assessment process and to examine the factors influencing industry's uptake of the VITAL(®) process...
March 14, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28294936/examining-the-feasibility-of-implementing-behavioural-economics-strategies-that-encourage-home-dinner-vegetable-intake-among-low-income-children
#13
Tashara M Leak, Alison Swenson, Aaron Rendahl, Zata Vickers, Elton Mykerezi, Joseph P Redden, Traci Mann, Marla Reicks
OBJECTIVE: To examine the feasibility of implementing nine behavioural economics-informed strategies, or 'nudges', that aimed to encourage home dinner vegetable intake among low-income children. DESIGN: Caregivers were assigned six of nine strategies and implemented one new strategy per week (i.e. 6 weeks) during three dinner meals. Caregivers recorded child dinner vegetable intake on the nights of strategy implementation and rated the level of difficulty for assigned strategies...
March 15, 2017: Public Health Nutrition
https://www.readbyqxmd.com/read/28294150/dietary-patterns-and-the-risk-of-rhinitis-in-primary-school-children-a-prospective-cohort-study
#14
Xudong Liu, Claudie Chiu-Yi Wong, Ignatius T S Yu, Zilong Zhang, Lixing Tan, Arthur P S Lau, Albert Lee, Eng Kiong Yeoh, Xiang Qian Lao
This study was to investigate the association between dietary patterns and rhinitis in primary school children. 1,599 students without rhinitis at baseline survey were selected from a primary school children cohort. Information on food consumption, respiratory symptoms, and confounders was collected using questionnaires. Dietary patterns were defined using principal component analysis. Logistic regression was performed to calculate odds ratio (OR) with 95% confidence intervals (95% CI). The incidence of rhinitis during 12 months follow-up was 21...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28294131/structural-and-functional-plasticity-within-the-nucleus-accumbens-and-prefrontal-cortex-associated-with-time-dependent-increases-in-food-cue-seeking-behavior
#15
Paige M Dingess, Rebecca A Darling, Rifka C Derman, Shaun S Wulff, Melissa L Hunter, Carrie R Ferrario, Travis E Brown
Urges to consume food can be driven by stimuli in the environment that are associated with previous food experience. Identifying adaptations within brain reward circuits that facilitate cue-induced food seeking is critical for understanding and preventing the overconsumption of food and subsequent weight gain. Utilizing electrophysiological, biochemical, and DiI labeling we examined functional and structural changes in the nucleus accumbens (NAc) and prefrontal cortex (PFC) associated with time-dependent increases in food craving ('incubation of craving')...
March 15, 2017: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28292487/a-review-of-patient-reported-outcome-labeling-in-the-united-states-2011-2015
#16
Ari Gnanasakthy, Margaret Mordin, Emily Evans, Lynda Doward, Carla DeMuro
BACKGROUND: A review of new drug approvals (NDAs) by the Food and Drug Administration (FDA) for 2006 to 2010 showed that 24.1% of new drugs had patient-reported outcome (PRO) labeling. OBJECTIVES: To review PRO labeling for NDAs for 2011 to 2015 and to compare key findings reported previously. METHODS: A review of the FDA drug approval reports for NDAs was conducted using the FDA Web site to determine the number of NDAs for the period 2011 to 2015...
March 2017: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/28290274/relative-bioavailability-of-an-empagliflozin-25-mg-linagliptin-5-mg-fixed-dose-combination-tablet%C3%A2
#17
Stephan Glund, Michaela Mattheus, Frank Runge, Peter Rose, Christian Friedrich
OBJECTIVE: This relative bioavailability study compared a fixed-dose combination (FDC) tablet of empagliflozin 25 mg/linagliptin 5 mg with the corresponding individual components. In addition, the effect of food on the bioavailability of the FDC was studied, and the standard-dissolving formulation FDC was compared with a slow-dissolving side batch. METHODS: An open-label, randomized, crossover study design was used (ClinicalTrials.gov Identifier NCT01189201). Healthy volunteers (n = 42) each received three single-dose treatments: FDC standard dissolution, individual tablets, and either FDC standard dissolution with food or FDC slow dissolution...
March 14, 2017: International Journal of Clinical Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28285197/graphene-oxide-functionalized-long-period-grating-for-ultrasensitive-label-free-immunosensing
#18
Chen Liu, Qi Cai, Baojian Xu, Weidong Zhu, Lin Zhang, Jianlong Zhao, Xianfeng Chen
We explore graphene oxide (GO) nanosheets functionalized dual-peak long period grating (dLPG) based biosensor for ultrasensitive label-free antibody-antigen immunosensing. The GO linking layer provides a remarkable analytical platform for bioaffinity binding interface due to its favorable combination of exceptionally high surface-to-volume ratio and excellent optical and biochemical properties. A new GO deposition technique based on chemical-bonding in conjunction with physical-adsorption was proposed to offer the advantages of a strong bonding between GO and fiber device surface and a homogeneous GO overlay with desirable stability, repeatability and durability...
March 9, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28284879/skipping-breakfast-reduces-energy-intake-and-physical-activity-in-healthy-women-who-are-habitual-breakfast-eaters-a-randomized-crossover-trial
#19
Eiichi Yoshimura, Yoichi Hatamoto, Satomi Yonekura, Hiroaki Tanaka
Many epidemiological studies indicate a positive relationship between skipping breakfast (SB) and obesity. However, it is unclear whether SB affects energy intake and physical activity during the day. The objective of the present study was to evaluate the acute effects of SB on energy intake and physical activity under free-living conditions. The present study used a randomized, crossover trial design comparing eating breakfast (EB) and SB days. Twenty lean, healthy women 21-25years old who were habitual breakfast eaters (≥5daysperweek) took part in this study...
March 9, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28283217/adolescents-with-at-risk-eating-and-lifestyle-behaviors-are-affected-by-after-school-schedules-across-the-clinical-weight-spectrum
#20
Leanna F Perez, Carla K Miller, Judith A Groner
OBJECTIVE: Evaluate adolescent lifestyle patterns to develop more effective health promotion programs. METHODS: An interview approach was employed to gain in-depth understanding of eating and activity behaviors. Adolescents aged 13-18 years (n=43) from clinically normal and obese weight categories were enrolled. Nutrient intake and food group servings were obtained from a food frequency questionnaire. RESULTS: Four participant subgroups were identified and labeled: "Idle, Engaged, Balanced and Working...
March 6, 2017: Patient Education and Counseling
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