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https://www.readbyqxmd.com/read/28107384/an-approach-to-elucidate-nbs1-function-in-dna-repair-using-frequent-nonsynonymous-polymorphism-in-wild-medaka-oryzias-latipes-populations
#1
Kento Igarashi, Junya Kobayashi, Takafumi Katsumura, Yusuke Urushihara, Kyohei Hida, Tomomi Watanabe-Asaka, Hiroki Oota, Shoji Oda, Hiroshi Mitani
Nbs1 is one of the genes responsible for Nijmegen breakage syndrome, which is marked with high radiosensitivity. In human NBS1 (hNBS1), Q185E polymorphism is known as the factor to cancer risks, although its DSB repair defect has not been addressed. Here we investigated the genetic variations in medaka (Oryzias latipes) wild populations, and found 40 nonsynonymous single nucleotide polymorphisms (SNPs) in medaka nbs1 (olnbs1) gene within 5 inbred strains. A mutation to histidine in Q170 residue in olNbs1, which corresponds to Q185 residue of hNBS1, was widely distributed in the closed colonies derived from the eastern Korean population of medaka...
2017: PloS One
https://www.readbyqxmd.com/read/28098238/expression-of-the-novel-maternal-centrosome-assembly-factor-wdr8-is-required-for-vertebrate-embryonic-mitoses
#2
Daigo Inoue, Manuel Stemmer, Thomas Thumberger, Thomas Ruppert, Felix Bärenz, Joachim Wittbrodt, Oliver J Gruss
The assembly of the first centrosome occurs upon fertilisation when male centrioles recruit pericentriolar material (PCM) from the egg cytoplasm. The mechanisms underlying the proper assembly of centrosomes during early embryogenesis remain obscure. We identify Wdr8 as a novel maternally essential protein that is required for centrosome assembly during embryonic mitoses of medaka (Oryzias latipes). By CRISPR-Cas9-mediated knockout, maternal/zygotic Wdr8-null (m/zWdr8(-/-)) blastomeres exhibit severe defects in centrosome structure that lead to asymmetric division, multipolar mitotic spindles and chromosome alignment errors...
January 18, 2017: Nature Communications
https://www.readbyqxmd.com/read/28093265/developmental-tracing-of-oocyte-development-in-gonadal-soma-derived-factor-deficiency-medaka-oryzias-latipes-using-a-transgenic-approach
#3
Guijun Guan, Kaiqing Sun, Xi Zhang, Xiaomiao Zha, Mingyou Li, Yan Yan, Yunzhi Wang, Jianbin Chen, Meisheng Yi, Yunhan Hong
Gonadal soma-derived factor (gsdf) is reported to be a male initiator in medaka based on loss- and gain- of function via targeted disruption, or transgenic over-expression. However, little is known about how gsdf promotes undifferentiated gonad entry into male pathways or prevents entry into the female pathway. We utilized a visible folliculogenesis system with a reporter cassette of dual-color fluorescence expression to identify difference between oocyte development from wildtype and gsdf deficiency medaka...
January 13, 2017: Mechanisms of Development
https://www.readbyqxmd.com/read/28087632/sequential-organogenesis-sets-two-parallel-sensory-lines-in-medaka
#4
Ali Seleit, Isabel Krämer, Elizabeth Ambrosio, Nicolas Dross, Ulrike Engel, Lázaro Centanin
Animal organs are typically formed during embryogenesis by following one specific developmental programme. Here we report that neuromast organs are generated by two distinct and sequential programmes that result in parallel sensory lines in medaka embryos. A ventral posterior lateral line (pLL) is composed of neuromasts deposited by collectively migrating cells while a midline pLL is formed by individually migrating cells. Despite the variable number of neuromasts among embryos, the sequential programmes that we describe here fix an invariable ratio between ventral and midline neuromasts...
January 13, 2017: Development
https://www.readbyqxmd.com/read/28077779/embryonic-exposure-to-tcdd-impacts-osteogenesis-of-the-axial-skeleton-in-japanese-medaka-oryzias-latipes
#5
AtLee T D Watson, Antonio Planchart, Carolyn J Mattingly, Christoph Winkler, David M Reif, Seth W Kullman
Recent studies from mammalian, fish, and in vitro models have identified bone and cartilage development as sensitive targets for dioxins and other aryl hydrocarbon receptor ligands. In this study, we assess how embryonic 2,3,7,8-tetrachlorochlorodibenzo-p-dioxin (TCDD) exposure impacts axial osteogenesis in Japanese medaka (Oryzias latipes), a vertebrate model of human bone development. Embryos from inbred wild-type Orange-red Hd-dR and 3 transgenic medaka lines (twist:EGFP, osx/sp7:mCherry, col10a1:nlGFP) were exposed to 0...
November 15, 2016: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28071684/genome-wide-identification-of-novel-ovarian-predominant-mirnas-new-insights-from-the-medaka-oryzias-latipes
#6
Amine Bouchareb, Aurélie Le Cam, Jérôme Montfort, Stéphanie Gay, Thaovi Nguyen, Julien Bobe, Violette Thermes
MicroRNAs (miRNAs) are small, highly conserved non-coding RNAs that play important roles in the regulation of many physiological processes. However, the role of miRNAs in vertebrate oocyte formation (i.e., oogenesis) remains poorly investigated. To gain new insights into the roles of miRNAs in oogenesis, we searched for ovarian-predominant miRNAs. Using a microarray displaying 3,800 distinct miRNAs originating from different vertebrate species, we identified 66 miRNAs that are expressed predominantly in the ovary...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28063342/linking-genomic-responses-of-gonads-with-reproductive-impairment-in-marine-medaka-oryzias-melastigma-exposed-chronically-to-the-chemopreventive-and-antifouling-agent-3-3-diindolylmethane-dim
#7
Lianguo Chen, Doris W T Au, Chenyan Hu, Weipeng Zhang, Bingsheng Zhou, Lin Cai, John P Giesy, Pei-Yuan Qian
3,3'-Diindolylmethane (DIM) has been promoted as an effective chemopreventive and antifouling additive. However, the concurrent risks or side effects of DIM are not fully understood, especially on tissues responsive to estrogen. Therefore, this study employed marine medaka (Oryzias melastigma) as a test model to evaluate relative safety and explore mechanisms of toxic action of DIM on development and function of gonad after chronic (28days) aqueous exposure to relatively low doses (0μg/L or 8.5μg/L). Integration of comprehensive toxicogenomic analysis at the transcriptome and proteome levels with apical endpoints, such as production of eggs and swimming performance of larvae, elucidated the molecular linkage in gonad from bottom up along the reproductive adverse outcome pathway...
December 30, 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/28061388/ancestral-benzo-a-pyrene-exposure-affects-bone-integrity-in-f3-adult-fish-oryzias-latipes
#8
Frauke Seemann, Chang-Bum Jeong, Ge Zhang, Miles Teng Wan, Baosheng Guo, Drew Ryan Peterson, Jae-Seong Lee, Doris Wai-Ting Au
Benzo[a]pyrene (BaP) at an environmentally relevant concentration (1μg/L) has previously been shown to affect bone development in a transgenerational manner in F3 medaka (Oryzias latipes) larvae (17dph). Here, we provide novel histomorphometric data demonstrating that the impaired bone formation at an early life stage is not recoverable and can result in a persistent transgenerational impairment of bone metabolism in F3 adult fish. A decrease in bone thickness and the occurrence of microcracks in ancestrally BaP-treated adult male fish (F3) were revealed by MicroCt measurement and histopathological analysis...
December 23, 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/28054105/comparative-developmental-toxicity-of-desalination-brine-and-sulfate-dominated-saltwater-in-a-euryhaline-fish
#9
Allison Kupsco, Rafid Sikder, Daniel Schlenk
Desalination is a promising sustainable solution to meet growing water needs of cities across the United States. However, the environmental impacts of the resulting filtrate (brine) discharged to surface water need to be evaluated before large-scale desalination can be successful in the United States. Developing fish are especially sensitive to changes in salinity and varying ionic composition. Limited research is available on the impacts of hypersalinity on chronic vertebrate embryonic development, particularly on sublethal effects...
January 4, 2017: Archives of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28026967/identification-of-molecular-targets-for-4-5-dichloro-2-n-octyl-4-isothiazolin-3-one-dcoit-in-teleosts-new-insight-into-mechanism-of-toxicity
#10
Lianguo Chen, Doris W T Au, Chenyan Hu, Drew R Peterson, Bingsheng Zhou, Pei-Yuan Qian
Environmental pollutants are capable of concomitantly inducing diverse toxic effects. However, it is largely unknown which effects are directly induced and which effects are secondary, thus calling for definitive identification of the initiating molecular event for a pollutant to elucidate the mechanism of toxicity. In present study, affinity pull-down assays were used to identify target proteins for 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT), a costal pollutant of emerging concern, in various tissues (e...
December 27, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/28013078/toxicity-of-oil-sands-acid-extractable-organic-fractions-to-freshwater-fish-pimephales-promelas-fathead-minnow-and-oryzias-latipes-japanese-medaka
#11
Anthony E Bauer, Richard A Frank, John V Headley, Kerry M Peru, Andrea J Farwell, D George Dixon
The Alberta oil sands are one of the largest global petroleum deposits and, due to non-release practices for oil sands process-affected waters, produced tailings are stored in large ponds. The acid extractable organic (AEO) compounds in oil sands process-affected water are of greatest concern due to their persistence and toxicity to a variety of aquatic biota. The present study evaluated the toxicity of the five AEO fractions to two fish species: Oryzias latipes (Japanese medaka) and Pimephales promelas (fathead minnow)...
December 14, 2016: Chemosphere
https://www.readbyqxmd.com/read/28013025/an-automated-assay-for-the-assessment-of-cardiac-arrest-in-fish-embryo
#12
Elodie Puybareau, Diane Genest, Emilie Barbeau, Marc Léonard, Hugues Talbot
Studies on fish embryo models are widely developed in research. They are used in several research fields including drug discovery or environmental toxicology. In this article, we propose an entirely automated assay to detect cardiac arrest in Medaka (Oryzias latipes) based on image analysis. We propose a multi-scale pipeline based on mathematical morphology. Starting from video sequences of entire wells in 24-well plates, we focus on the embryo, detect its heart, and ascertain whether or not the heart is beating based on intensity variation analysis...
December 15, 2016: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28004797/acute-transcriptional-up-regulation-specific-to-osteoblasts-osteoclasts-in-medaka-fish-immediately-after-exposure-to-microgravity
#13
Masahiro Chatani, Hiroya Morimoto, Kazuhiro Takeyama, Akiko Mantoku, Naoki Tanigawa, Koji Kubota, Hiromi Suzuki, Satoko Uchida, Fumiaki Tanigaki, Masaki Shirakawa, Oleg Gusev, Vladimir Sychev, Yoshiro Takano, Takehiko Itoh, Akira Kudo
Bone loss is a serious problem in spaceflight; however, the initial action of microgravity has not been identified. To examine this action, we performed live-imaging of animals during a space mission followed by transcriptome analysis using medaka transgenic lines expressing osteoblast and osteoclast-specific promoter-driven GFP and DsRed. In live-imaging for osteoblasts, the intensity of osterix- or osteocalcin-DsRed fluorescence in pharyngeal bones was significantly enhanced 1 day after launch; and this enhancement continued for 8 or 5 days...
December 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27993982/a-vertebrate-specific-and-essential-role-for-osterix-in-osteogenesis-revealed-by-gene-knockout-in-the-teleost-medaka
#14
Tingsheng Yu, Martin Graf, Joerg Renn, Manfred Schartl, Daria Larionova, Ann Huysseune, Paul Eckhard Witten, Christoph Winkler
osterix (osx; sp7) encodes a zinc-finger transcription factor that controls osteoblast differentiation in mammals. Although identified in all vertebrate lineages, its role in non-mammalian bone formation remains elusive. Here, we show that an osx mutation in medaka results in severe bone defects and larval lethality. Pre-osteoblasts fail to differentiate leading to severe intramembranous and perichondral ossification defects. The notochord sheath mineralizes normally, supporting the idea of an osteoblast-independent mechanism for teleost vertebral centra formation...
January 15, 2017: Development
https://www.readbyqxmd.com/read/27990605/profiling-of-selected-functional-metabolites-in-the-central-nervous-system-of-marine-medaka-oryzias-melastigma-for-environmental-neurotoxicological-assessments
#15
Elva Ngai-Yu Lei, Man-Shan Yau, Chi-Chung Yeung, Margaret B Murphy, Ka-Leung Wong, Michael Hon-Wah Lam
The simultaneous profiling of 43 functional metabolites in the brain of the small model vertebrate organism, marine medaka (Oryzais melastigma), has been accomplished via dansyl chloride derivatization and LC-MS/MS quantification. This technique was applied to examine effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47), one of the most abundant polybrominated diphenyl ether flame retardants in the natural environment, on the central nervous system (CNS) of vertebrates. The model teleosts were fed with bioencapsulated Artemia nauplii for up to 21 days...
December 18, 2016: Archives of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/27966251/identification-and-conservation-of-gene-loss-events-of-hox-gene-clusters-in-the-marine-medaka-oryzias-melastigma
#16
Hui-Su Kim, Dae-Sik Hwang, Chang-Bum Jeong, Doris W T Au, Jae-Seong Lee
In this study, the identification of the whole Hox gene clusters (46 Hox genes) in the marine medaka Oryzias melastigma was investigated using genome assembly and RNA-seq information. Moreover, the gene loss events of Hox gene clusters, which may occur during fish evolution, were examined for a better understanding of the evolutionary status of the gene lost events of the Hox gene cluster across fish species, particularly in the genus Oryzias.
November 2016: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/27930992/alteration-of-development-and-gene-expression-induced-by-in-ovo-nanoinjection-of-3-hydroxybenzo-c-phenanthrene-into-japanese-medaka-oryzias-latipes-embryos
#17
Kun Chen, Yuki Tsutsumi, Shuhei Yoshitake, Xuchun Qiu, Hai Xu, Yasuyuki Hashiguchi, Masato Honda, Kosuke Tashiro, Kei Nakayama, Takeshi Hano, Nobuo Suzuki, Kazuichi Hayakawa, Yohei Shimasaki, Yuji Oshima
Benzo[c]phenanthrene (BcP) is a highly toxic polycyclic aromatic hydrocarbon (PAHs) found throughout the environment. In fish, it is metabolized to 3-hydroxybenzo[c]phenanthrene (3-OHBcP). In the present study, we observed the effects of 1nM 3-OHBcP on the development and gene expression of Japanese medaka (Oryzias latipes) embryos. Embryos were nanoinjected with the chemical after fertilization. Survival, developmental stage, and heart rate of the embryos were observed, and gene expression differences were quantified by messenger RNA sequencing (mRNA-Seq)...
January 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/27929129/subchronic-perfluorooctanesulfonate-pfos-exposure-induces-elevated-mutant-frequency-in-an-in-vivo-%C3%AE-transgenic-medaka-mutation-assay
#18
Yuanhong Chen, Wei Hu, Changjiang Huang, Shushan Hua, Qihao Wei, Chenglian Bai, Jiangfei Chen, Michelle B Norris, Richard Winn, Dongren Yang, Qiaoxiang Dong
Perfluorooctanesulfonate (PFOS) has been widely detected in the environment, wildlife and humans, but few studies have ever examined its mutagenic effect in vivo. In the present study, we use a transgenic fish model, the λ transgenic medaka, to evaluate the potential mutagenicity of PFOS in vivo following a subchronic exposure of 30 days. The mutant frequency of cII target gene was 3.46 × 10(-5) in liver tissue from control fish, which increased by 1.4-fold to 4.86 × 10(-5) in fish exposed to 6.7 μg/L PFOS, 1...
December 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27897263/principal-function-of-mineralocorticoid-signaling-suggested-by-constitutive-knockout-of-the-mineralocorticoid-receptor-in-medaka-fish
#19
Tatsuya Sakamoto, Madoka Yoshiki, Hideya Takahashi, Masayuki Yoshida, Yukiko Ogino, Toshitaka Ikeuchi, Tomoya Nakamachi, Norifumi Konno, Kouhei Matsuda, Hirotaka Sakamoto
As in osmoregulation, mineralocorticoid signaling is implicated in the control of brain-behavior actions. Nevertheless, the understanding of this role is limited, partly due to the mortality of mineralocorticoid receptor (MR)-knockout (KO) mice due to impaired Na(+) reabsorption. In teleost fish, a distinct mineralocorticoid system has only been identified recently. Here, we generated a constitutive MR-KO medaka as the first adult-viable MR-KO animal, since MR expression is modest in osmoregulatory organs but high in the brain of adult medaka as for most teleosts...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27875720/multi-generational-xenoestrogenic-effects-of-perfluoroalkyl-acids-pfaas-mixture-on-oryzias-latipes-using-a-flow-through-exposure-system
#20
Jin Wuk Lee, Jae-Woo Lee, Yu-Jin Shin, Ji-Eun Kim, Tae-Kwon Ryu, Jisung Ryu, Jaean Lee, Pilje Kim, Kyunghee Choi, Kyunghwa Park
To elucidate the multi-generational estrogenic potential of Perfluoroalkyl acids (PFAAs) mixture, vitellogenin (VTG) expression, growth indices, histological alteration, fecundity, hatching rate, larval survival rate, and sex ratio of Japanese medaka (Oryzias latipes) were investigated by exposing the fish to a mixture of perfluorooctane sulfonate (PFOS), perfluroroctanoic acid (PFOA), perfluorobutane sulfonate (PFBS), and perfluorononanoic acid (PFNA) for three generations (238 days). Mixture composition is in the ratio of 1:1:1:1...
February 2017: Chemosphere
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