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https://www.readbyqxmd.com/read/27930992/alteration-of-development-and-gene-expression-induced-by-in-ovo-nanoinjection-of-3-hydroxybenzo-c-phenanthrene-into-japanese-medaka-oryzias-latipes-embryos
#1
Kun Chen, Yuki Tsutsumi, Shuhei Yoshitake, Xuchun Qiu, Hai Xu, Yasuyuki Hashiguchi, Masato Honda, Kosuke Tashiro, Kei Nakayama, Takeshi Hano, Nobuo Suzuki, Kazuichi Hayakawa, Yohei Shimasaki, Yuji Oshima
Benzo[c]phenanthrene (BcP) is a highly toxic polycyclic aromatic hydrocarbon (PAHs) found throughout the environment. In fish, it is metabolized to 3-hydroxybenzo[c]phenanthrene (3-OHBcP). In the present study, we observed the effects of 1nM 3-OHBcP on the development and gene expression of Japanese medaka (Oryzias latipes) embryos. Embryos were nanoinjected with the chemical after fertilization. Survival, developmental stage, and heart rate of the embryos were observed, and gene expression differences were quantified by messenger RNA sequencing (mRNA-Seq)...
November 25, 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27929129/subchronic-perfluorooctanesulfonate-pfos-exposure-induces-elevated-mutant-frequency-in-an-in-vivo-%C3%AE-transgenic-medaka-mutation-assay
#2
Yuanhong Chen, Wei Hu, Changjiang Huang, Shushan Hua, Qihao Wei, Chenglian Bai, Jiangfei Chen, Michelle B Norris, Richard Winn, Dongren Yang, Qiaoxiang Dong
Perfluorooctanesulfonate (PFOS) has been widely detected in the environment, wildlife and humans, but few studies have ever examined its mutagenic effect in vivo. In the present study, we use a transgenic fish model, the λ transgenic medaka, to evaluate the potential mutagenicity of PFOS in vivo following a subchronic exposure of 30 days. The mutant frequency of cII target gene was 3.46 × 10(-5) in liver tissue from control fish, which increased by 1.4-fold to 4.86 × 10(-5) in fish exposed to 6.7 μg/L PFOS, 1...
December 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27897263/principal-function-of-mineralocorticoid-signaling-suggested-by-constitutive-knockout-of-the-mineralocorticoid-receptor-in-medaka-fish
#3
Tatsuya Sakamoto, Madoka Yoshiki, Hideya Takahashi, Masayuki Yoshida, Yukiko Ogino, Toshitaka Ikeuchi, Tomoya Nakamachi, Norifumi Konno, Kouhei Matsuda, Hirotaka Sakamoto
As in osmoregulation, mineralocorticoid signaling is implicated in the control of brain-behavior actions. Nevertheless, the understanding of this role is limited, partly due to the mortality of mineralocorticoid receptor (MR)-knockout (KO) mice due to impaired Na(+) reabsorption. In teleost fish, a distinct mineralocorticoid system has only been identified recently. Here, we generated a constitutive MR-KO medaka as the first adult-viable MR-KO animal, since MR expression is modest in osmoregulatory organs but high in the brain of adult medaka as for most teleosts...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27875720/multi-generational-xenoestrogenic-effects-of-perfluoroalkyl-acids-pfaas-mixture-on-oryzias-latipes-using-a-flow-through-exposure-system
#4
Jin Wuk Lee, Jae-Woo Lee, Yu-Jin Shin, Ji-Eun Kim, Tae-Kwon Ryu, Jisung Ryu, Jaean Lee, Pilje Kim, Kyunghee Choi, Kyunghwa Park
To elucidate the multi-generational estrogenic potential of Perfluoroalkyl acids (PFAAs) mixture, vitellogenin (VTG) expression, growth indices, histological alteration, fecundity, hatching rate, larval survival rate, and sex ratio of Japanese medaka (Oryzias latipes) were investigated by exposing the fish to a mixture of perfluorooctane sulfonate (PFOS), perfluroroctanoic acid (PFOA), perfluorobutane sulfonate (PFBS), and perfluorononanoic acid (PFNA) for three generations (238 days). Mixture composition is in the ratio of 1:1:1:1...
November 19, 2016: Chemosphere
https://www.readbyqxmd.com/read/27862951/characterization-and-evolution-of-the-spotted-gar-retina
#5
Joshua M Sukeena, Carlos A Galicia, Jacob D Wilson, Tim McGinn, Janette W Boughman, Barrie D Robison, John H Postlethwait, Ingo Braasch, Deborah L Stenkamp, Peter G Fuerst
In this study, we characterize the retina of the spotted gar, Lepisosteus oculatus, a ray-finned fish. Gar did not undergo the whole genome duplication event that occurred at the base of the teleost fish lineage, which includes the model species zebrafish and medaka. The divergence of gars from the teleost lineage and the availability of a high-quality genome sequence make it a uniquely useful species to understand how genome duplication sculpted features of the teleost visual system, including photoreceptor diversity...
November 9, 2016: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/27861979/targeted-inactivation-of-dna-photolyase-genes-in-medaka-fish-oryzias-latipes
#6
Tomoko Ishikawa-Fujiwara, Eri Shiraishi, Yoshihiro Fujikawa, Toshio Mori, Tohru Tsujimura, Takeshi Todo
Proteins of the cryptochrome/photolyase family (CPF) exhibit sequence and structural conservation, but their functions are divergent. Photolyase is a DNA repair enzyme that catalyzes the light-dependent repair of ultraviolet (UV)-induced photoproducts, whereas cryptochrome acts as a photoreceptor or circadian clock protein. Two types of DNA photolyase exist: CPD photolyase, which repairs cyclobutane pyrimidine dimers (CPDs), and 6-4 photolyase, which repairs 6-4 pyrimidine-pyrimidone photoproducts (6-4PPs)...
November 10, 2016: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/27858334/identification-of-medaka-magnetoreceptor-and-cryptochromes
#7
Yunzhi Wang, Jianbin Chen, Feng Zhu, Yunhan Hong
Magnetoreception is a hallmark ability of animals for orientation and migration via sensing and utilizing geomagnetic fields. Magnetoreceptor (MagR) and cryptochromes (Cry) have recently been identified as the basis for magnetoreception in Drosophila. However, it has remained unknown whether MagR and Cry have conserved roles in diverse animals. Here we report the identification and expression of magr and cry genes in the fish medaka (Oryzias latipes). Cloning and sequencing identified a single magr gene, four cry genes and one cry-like gene in medaka...
November 17, 2016: Science China. Life Sciences
https://www.readbyqxmd.com/read/27855535/biochemical-characterization-of-the-medaka-oryzias-latipes-orthologue-for-mammalian-tissue-type-transglutaminase-tg2
#8
Yuki Takada, Yuko Watanabe, Kazuho Okuya, Hideki Tatsukawa, Hisashi Hashimoto, Kiyotaka Hitomi
Transglutaminase is an enzyme family responsible for post-translational modification such as protein cross-linking and the attachment of primary amine and/or deamidation of glutamine-residue in proteins. Medaka (Oryzias latipes), a recently established model fish, has similar functional proteins to those characterized in mammals. Previously, we found the apparent orthologues that correspond to human transglutaminases in medaka. In this study, regarding the medaka orthologue of human tissue-type transglutaminase (OlTGT), recombinant protein was expressed in an active form in bacteria cultured at low temperature...
November 18, 2016: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/27825821/constitutive-overexpressed-type-i-interferon-induced-downregulation-of-antiviral-activity-in-medaka-fish-oryzias-latipes
#9
Shun Maekawa, Takashi Aoki, Han-Ching Wang
In fish, as well as vertebrates, type I interferons (IFNs) are important cytokines that help to provide innate, antiviral immunity. Although low amounts of IFN are constitutively secreted under normal physiological conditions, long-term and excessive IFN stimulation leads to reduced sensitivity to the IFN signal. This provides a negative feedback mechanism that prevents inappropriate responses and autoimmunity. At present, however, neither IFN desensitization nor the normal physiological role of constitutive IFN are well characterized in fish...
November 5, 2016: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/27824153/conspecific-injury-raises-an-alarm-in-medaka
#10
Ajay S Mathuru
In the late 1930s, Karl von Frisch reported that semiochemicals released upon injury, act as alarm substances (Schreckstoff) in fish. In Ostariophysi species, club cells in the epidermis are believed to contain cues related to alarm substance; however, the function of club cells, primarily as reservoirs of alarm substance has been debated. Here, I describe an alarm response in the Japanese rice fish Oryzias latipes (medaka), a member of the order Beloniformes. The response to alarm substance (Schreckreaction) in medaka is characterized by bouts of immobility and an increase in cortisol levels within minutes of exposure to conspecific skin extract...
November 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27814527/an-integrated-omic-analysis-of-hepatic-alteration-in-medaka-fish-chronically-exposed-to-cyanotoxins-with-possible-mechanisms-of-reproductive-toxicity
#11
Qin Qiao, Séverine Le Manach, Hélène Huet, Evelyne Duvernois-Berthet, Soraya Chaouch, Charlotte Duval, Benoit Sotton, Loïc Ponger, Arul Marie, Lucrèce Mathéron, Sarah Lennon, Gérard Bolbach, Chakib Djediat, Cécile Bernard, Marc Edery, Benjamin Marie
Cyanobacterial blooms threaten human health as well as the population of other living organisms in the aquatic environment, particularly due to the production of natural toxic components, the cyanotoxin. So far, the most studied cyanotoxins are microcystins (MCs). In this study, the hepatic alterations at histological, proteome and transcriptome levels were evaluated in female and male medaka fish chronically exposed to 1 and 5 μg L(-1) microcystin-LR (MC-LR) and to the extract of MC-producing Microcystis aeruginosa PCC 7820 (5 μg L(-1) of equivalent MC-LR) by balneation for 28 days, aiming at enhancing our understanding of the potential reproductive toxicity of cyanotoxins in aquatic vertebrate models...
October 26, 2016: Environmental Pollution
https://www.readbyqxmd.com/read/27810580/the-influence-of-control-group-reproduction-on-the-statistical-power-of-the-environmental-protection-agency-s-medaka-extended-one-generation-reproduction-test-meogrt
#12
Kevin Flynn, Joe Swintek, Rodney Johnson
Because of various Congressional mandates to protect the environment from endocrine disrupting chemicals (EDCs), the United States Environmental Protection Agency (USEPA) initiated the Endocrine Disruptor Screening Program. In the context of this framework, the Office of Research and Development within the USEPA developed the Medaka Extended One Generation Reproduction Test (MEOGRT) to characterize the endocrine action of a suspected EDC. One important endpoint of the MEOGRT is fecundity of medaka breeding pairs...
October 27, 2016: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27810490/bioconcentration-metabolism-and-biomarker-responses-in-marine-medaka-oryzias-melastigma-exposed-to-sulfamethazine
#13
Songhe Zhao, Xinhong Wang, Yongyu Li, Jianqing Lin
The antibiotic sulfamethazine (SM2) is commonly used in agriculture and livestock for its broad-spectrum antibacterial properties. Due to its widespread application, SM2 is frequently detected in surface water and sediments. The objective of this study was to investigate the bioconcentration, distribution and biomarker responses of SM2 and its main metabolite, acetylated sulfamethazine (N-SM2) in medaka (Oryzias melastigma). Two treated groups of medaka were exposed to concentrations of 40μg/L and 200μg/L of SM2 for 24h to simulate the habitual use of those antibiotics in aquiculture activities...
December 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27780364/bile-acids-and-their-oxo-derivatives-environmentally-safe-materials-for-drug-design-and-delivery
#14
Jovana Trifunović, Vladan Borčić, Saša Vukmirović, Velibor Vasović, Momir Mikov
PURPOSE: Animal tests have been often used in toxicology to determine parameters describing toxicity of a particular substance. However, in vivo tests must fulfill ethical requirements, and are both time and money consuming. Therefore, computational methods are considered to be very useful in toxicity prediction. METHODS: Retention parameters were acquired by normal-phase TLC. Lipophilicity was used as a key parameter for predicting toxic potential. The correlation coefficients between calculated log P values obtained by five different software and experimentally determined hydrophobicity parameters ([Formula: see text](tol/et), [Formula: see text](tol/but), b(tol/et) and b(tol/but)) were calculated...
October 26, 2016: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/27774651/toxicokinetics-and-toxicodynamics-of-chlorpyrifos-is-altered-in-embryos-of-japanese-medaka-exposed-to-oil-sands-process-affected-water-evidence-for-inhibition-of-p-glycoprotein
#15
Hattan A Alharbi, Jane Alcorn, Ahmed Al-Mousa, John P Giesy, Steve B Wiseman
Oil sands process-affected water (OSPW) is generated during extraction of bitumen in the surface mining oil sands industry in Alberta, Canada. Studies were performed in vitro by use of Caco-2 cells, and in vivo with larvae of Japanese medaka (Oryzias latipes) to determine if organic compounds from the aqueous phase of OSPW inhibit ATP binding cassette protein ABCB1 (permeability-glycoprotein, P-gp). Neutral and basic fractions of OSPW inhibited activity of P-gp in Caco-2 cells by 1.9- and 2.0-fold, respectively, while the acidic fraction had the least effect...
October 24, 2016: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/27771993/identification-prokaryote-expression-of-medaka-gdnfa-b-and-their-biological-activity-in-a-spermatogonial-cell-line
#16
Jing Wei, Linyan Liu, Zhenhua Fan, Yunhan Hong, Yang Zhao, Linyan Zhou, Deshou Wang
The origin and evolution of molecular mechanisms underlying the self-renewal and differentiation of spermatogonial stem cells (SSC) are fundamental questions in stem cell biology as well as reproduction medicine. In mammals, glial cell line-derived neurotrophic factor (GDNF) is crucial for SSC self-renewal and maintenance. However, in non-mammals, the role of Gdnf in SSC still remains unknown. Here we report that the two GDNF homologs from medaka fish (Oryzias latipes), namely OlGdnfa and OlGdnfb, can promote the proliferation activity and retain the spermatogonial property of SG3, a spermatogonial cell line derived from adult medaka showing the intrinsic property of SSC by self-renewal and differentiation potential during 2 years of culture...
October 22, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27768946/hypoxia-alters-testicular-functions-of-marine-medaka-through-micrornas-regulation
#17
Anna Chung-Kwan Tse, Jing-Woei Li, Simon Yuan Wang, Ting-Fung Chan, Keng Po Lai, Rudolf Shiu-Sun Wu
Hypoxia is a global environmental concern and poses a significant threat to aquatic ecosystems, including the sustainability of natural fish populations. The deleterious effects of hypoxia on fish reproductive fitness, as mediated by disruption of sex hormones and gene expression along the Brain-Pituitary-Gonad axis, have been well documented. Recently, we further demonstrated that the observed disruption of steroidogenesis in the ovary of marine medaka Oryzias melastigma is mediated through microRNAs (miRNAs)...
November 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27759163/generation-of-a-transgenic-medaka-oryzias-latipes-strain-for-visualization-of-nuclear-dynamics-in-early-developmental-stages
#18
Takanobu Inoue, Atsuo Iida, Shingo Maegawa, Atsuko Sehara-Fujisawa, Masato Kinoshita
In this study, we verified nuclear transport activity of an artificial nuclear localization signal (aNLS) in medaka fish (Oryzias latipes). We generated a transgenic medaka strain expresses the aNLS tagged enhanced green fluorescent protein (EGFP) driven by a medaka beta-actin promoter. The aNLS-EGFP was accumulated in the nuclei of somatic tissues and yolk nuclei of oocytes, but undetectable in the spermatozoa. The fluorescent signal was observed from immediately after fertilization by a maternal contribution...
October 19, 2016: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/27734936/metabolomics-approach-reveals-metabolic-disorders-and-potential-biomarkers-associated-with-the-developmental-toxicity-of-tetrabromobisphenol-a-and-tetrachlorobisphenol-a
#19
Guozhu Ye, Yajie Chen, Hong-Ou Wang, Ting Ye, Yi Lin, Qiansheng Huang, Yulang Chi, Sijun Dong
Tetrabromobisphenol A and tetrachlorobisphenol A are halogenated bisphenol A (H-BPA), and has raised concerns about their adverse effects on the development of fetuses and infants, however, the molecular mechanisms are unclear, and related metabolomics studies are limited. Accordingly, a metabolomics study based on gas chromatography-mass spectrometry was employed to elucidate the molecular developmental toxicology of H-BPA using the marine medaka (Oryzias melastigmas) embryo model. Here, we revealed decreased synthesis of nucleosides, amino acids and lipids, and disruptions in the TCA (tricarboxylic acid) cycle, glycolysis and lipid metabolism, thus inhibiting the developmental processes of embryos exposed to H-BPA...
October 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27720471/physiological-and-behavioural-impacts-of-pacific-ciguatoxin-1-p-ctx-1-on-marine-medaka-oryzias-melastigma
#20
Yim Ling Mak, Jing Li, Chih-Ning Liu, Shuk Han Cheng, Paul K S Lam, Jinping Cheng, Leo L Chan
Ciguatoxins (CTXs) are natural biotoxins produced by benthic dinoflagellates of the genus Gambierdiscus, which are bioaccumulated and biotransformed along food chains in coral ecosystems. They are neurotoxins that activate voltage-gated sodium channels and disrupt ion conductance in the excitable tissues. Pacific ciguatoxin-1 (P-CTX-1) is the most prevalent and potent CTX congener present in fishes from the Pacific Ocean. In this study, P-CTX-1 was administrated to larval marine medaka (2h post-hatch) via microinjection...
October 1, 2016: Journal of Hazardous Materials
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