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Pei-Han Liao, Wen-Kai Yang, Ching-Hsin Yang, Chun-Hon Lin, Chin-Chu Hwang, Pei-Jen Chen
With increasing problems of drug abuse worldwide, aquatic ecosystems are contaminated by human pharmaceuticals from the discharge of hospital or municipal effluent. However, ecotoxicity data and related toxic mechanism for neuroactive controlled or illicit drugs are still lacking, so assessing the associated hazardous risk is difficult. This study aims to investigate the behavioral changes, oxidative stress, gene expression and neurotoxic or apoptosis effect(s) in larvae of medaka fish (Oryzias latipes) with environmentally relevant exposures of ketamine (KET) solutions for 1-14 days...
November 13, 2017: Environmental Pollution
Spencer R Saraf, Amy Frenkel, Matthew J Harke, Jennifer G Jankowiak, Christopher J Gobler, Anne E McElroy
Freshwater cyanobacterial harmful algal blooms (CyanoHABs) caused by algae in the genus Microcystis have been increasing in frequency and severity in recent decades. Microcystis blooms threaten aquatic organisms through effects associated with the rapid increase of biomass and the production of the hepatotoxin microcystin (MC) by toxic strains. Among fish, effects of blooms are likely to be more severe for early life stages, and physiological impacts on this life stage could significantly impact recruitment and fish populations...
November 2, 2017: Aquatic Toxicology
E Sarropoulou, A Y M Sundaram, E Kaitetzidou, G Kotoulas, G D Gilfillan, N Papandroulakis, C C Mylonas, A Magoulas
Background: Teleosts of the genus Seriola, commonly known as amberjacks, are of high commercial value in international markets due to their flesh quality and worldwide distribution. The Seriola species of interest to Mediterranean aquaculture is the greater amberjack (Seriola dumerili). This species holds great potential for the aquaculture industry, but in captivity, reproduction has proved to be challenging and observed growth dysfunction hinders their domestication. Insights into molecular mechanisms may contribute to a better understanding of traits like growth and sex, but investigations to unravel the molecular background of amberjacks have begun only recently...
November 8, 2017: GigaScience
Salvador Sastre, Carlos Fernández Torija, Gregoria Carbonell, José Antonio Rodríguez Martín, Eulalia María Beltrán, Miguel González-Doncel
A diet fortified with 2,2', 4,4'-tetrabromodiphenyl ether (BDE-47: 0, 10, 100, and 1000 ng/g) was dosed to 4-7-day-old post-hatch medaka fish for 40 days to evaluate the effects on the swimming activity of fish using a miniaturized swimming flume. Chlorpyrifos (CF)-exposed fish were selected as the positive control to assess the validity and sensitivity of the behavioral findings. After 20 and 40 days of exposure, the locomotor activity was analyzed for 6 min in a flume section (arena). The CF positive control for each time point were fish exposed to 50 ng CF/ml for 48 h...
November 2, 2017: Environmental Pollution
Ayano Hirako, Yuki Takeoka, Satoshi Furukawa, Akihiko Sugiyama
For the purpose of clarifying the histopathological effects of methotrexate (MTX) on medaka testes, wild-type and homogenic p53-deficient male medaka at 4 to 6 months post-hatching were exposed to 0.25 mg/ml of MTX for 96 h with histopathological examination of testes at 24, 48, 72 and 96 h. At 72 and 96 h after the start of MTX exposure, numerous apoptotic cells were observed in the spermatogonia and spermatocytes, and the pyknotic cell rate and the TUNEL-positive and cleaved caspase-3-positive rates in the spermatogonia and spermatocytes of MTX-treated wild type medaka were higher compared with those in the control wild-type medaka...
October 2017: Journal of Toxicologic Pathology
Hongyan Xu, Caixia Li, Pacharaporn Suklai, Qinghua Zeng, Raymond Chong, Zhiyuan Gong
It has been intensively documented that there are species-differences in the sensitivity to dioxin-like compounds (DLCs) in mammalian and avian. However, this issue is still unclear in fish. This study aimed at evaluating the differential sensitivities to DLCs in fish larvae. Here, larvae of Tg(cyp1a:gfp) medaka and Tg(cyp1a:gfp) zebrafish were tested with 2,3,7,8-Tetrachlorodibenzodioxin (TCDD), polychlorinated biphenyl 126 (PCB 126) and 2,3,4,7,8,-Pentachlorodibenzofuran (PeCDF). Comparative analyses were performed on induction of GFP fluorescence, expression of endogenous cyp1a mRNAs and EROD activity between the two species after exposure to these chemicals...
October 29, 2017: Chemosphere
Hongxing Chen, Zhongbao Guo, Yu Zhou, Dan Li, Lei Mu, Paul L Klerks, Yongju Luo, Lingtian Xie
We previously demonstrated that chronic exposure to hexavalent chromium (Cr(VI)) causes a variety of adverse effects in the Japanese medaka (Oryzias latipes). The present study investigated the transition of acute to chronic effects by assessing the influences of Cr(VI) exposure concentration and exposure duration on Cr accumulation and their effects on fish growth and antioxidant physiology. Juvenile fish were exposed to 0.05, 0.5, 4 or 8mg/L Cr(VI) for 28 days. Endpoints were evaluated on days 3, 7, 14, 21 and 28...
October 22, 2017: Ecotoxicology and Environmental Safety
Mikoto Nakajo, Shinji Kanda, Tomomi Karigo, Akiko Takahashi, Yasuhisa Akazome, Yoshihisa Uenoyama, Makito Kobayashi, Yoshitaka Oka
Kisspeptin neuronal system, which consists of a neuropeptide kisspeptin and its receptor Gpr54, is considered in mammals a key factor of reproductive regulation, so called hypothalamic-pituitary-gonadal (HPG) axis. However, in non-mammalian vertebrates, especially in teleosts, existence of kisspeptin regulation on HPG axis is still controversial. Here, we applied multidisciplinary techniques to a teleost fish, medaka and examined possible kisspeptin regulation on HPG axis. First, we generated knockout medaka for kisspeptin-related genes and found that they show normal fertility, gonadal maturation, and expression of gonadotropins...
October 18, 2017: Endocrinology
Yuriko Matsuzaki, Tetsushi Sakuma, Takashi Yamamoto, Hideyuki Saya
Phosphatase and tensin homolog (PTEN) is a lipid and protein phosphatase that antagonizes signaling by the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway. The PTEN gene is a major tumor suppressor, with mutations of this gene occurring frequently in tumors of humans and mice. We have now developed mutant medaka deficient in PTEN with the use of transcription activator-like effector nuclease (TALEN) technology. Medaka possesses two pten genes, ptena and ptenb, similar to zebrafish. We established 16 ptena mutant lines and two ptenb mutant lines...
2017: PloS One
Jian Wang, Aili Wang, Wen-Xiong Wang
Waterborne nanotoxicology of zinc oxide nanoparticles (nano-ZnOs) has been extensively studied over the past decade, whereas their potential dietary toxicity and applications were seldom investigated. In the present study, we systematically investigated both short-term bioavailability and chronic effects of nano-ZnOs to two marine fish (marine medaka Oryzias melastigma and red drum Sciaenops ocellatus). At normal supplementary level (80 mg Zn/kg), red drum (with a stomach) had similar assimilation efficiencies of nano-ZnOs and ZnCl2...
October 20, 2017: Nanotoxicology
G Janelle Espinoza, J Michael Poland, Jaime R Alvarado Bremer
Genotyping fish larvae is a valuable technique for numerous fields of study. While methods for collecting DNA from early stage larvae have been published, a non-lethal, non-invasive genotyping protocol for hatchlings that is amenable to high-throughput approaches is desirable. Here, we describe a method to individually genotype live, free-swimming, early fish larvae by characterizing their environmental DNA (eDNA). We demonstrate the utility of the method by assigning parentage to a sample (n = 50) of 3-5-day-old sheepshead minnow (Cyprinodon variegatus) larvae hatchlings, with very high rates of genotyping success (98%) and survival (92%) using mitochondrial and microsatellite DNA data...
October 1, 2017: BioTechniques
Isabelle Leguen
The general morphology and surface ultrastructure of the gills of adult and larvae medaka (Oryzias latipes) were studied in freshwater and seawater using scanning electron microscopy. The gills of all examined fish were structurally similar to those of other teleosts and consisted of four pairs of arches supporting (i) filaments bearing lamellae and (ii) rakers containing taste buds. Three cell types, specifically pavement cells, mitochondria-rich cells (MRCs), and mucous cells, constituted the surface layer of the gill epithelium...
October 17, 2017: Journal of Morphology
Mai Oda, Kan Saito, Shun Hatta, Yoshihiro Kubo, Osamu Saitoh
TRPA1 of insects and several tetrapod vertebrates except for those of rodents have been reported to be activated by noxious chemicals and also by high temperature with a relatively clear threshold. We previously analyzed the characteristics of two TRPA1 paralogs of zebrafish (zTRPA1a, b) and demonstrated that zTRPA1a is specialized for chemical sensing while zTRPA1b responds to thermal stimulations, that zTRPA1b responds to both cold and heat stimuli, and that heat stimulation gradually activates zTRPA1b without a clear threshold...
October 13, 2017: Biochemical and Biophysical Research Communications
Chisato Kataoka, Yumie Kato, Tadashi Ariyoshi, Masaki Takasu, Takahito Narazaki, Seiji Nagasaka, Haruki Tatsuta, Shosaku Kashiwada
Fish embryo toxicology is important because embryos are more susceptible than adults to toxicants. In addition, the aquatic toxicity of chemicals depends on water quality. We examined the toxicities to medaka embryos of three types of silver-AgNO3, silver nanocolloids (SNCs), and silver ions from silver nanoparticle plates (SNPPs)-under three pH conditions (4.0, 7.0, and 9.0) in embryo-rearing medium (ERM) or ultrapure water. Furthermore, we tested the later-life-stage effects of SNCs on medaka and their population growth...
October 13, 2017: Environmental Pollution
Junpei Yamashita, Yukika Kawabata, Kataaki Okubo
Oxytocin, a mammalian neuropeptide primarily synthesized in the supraoptic and paraventricular nuclei of the hypothalamus, mediates a variety of physiological and behavioral processes ranging from parturition and lactation to affiliation and prosociality. Multiple studies in rodents have shown that the expression of the oxytocin gene (Oxt) is stimulated by estrogen, while androgen has no apparent effect. However, this finding is not consistent across all studies, and no study has examined sex steroid regulation of Oxt or its orthologs in other animals...
October 10, 2017: Journal of Neuroendocrinology
Mikki Boswell, William Boswell, Yuan Lu, Markita Savage, Zachary Mazurek, Jordan Chang, Jeanot Muster, Ronald Walter
Differences in light sources are common in animal facilities and potentially can impact experimental results. Here, the potential impact of lighting differences on skin transcriptomes has been tested in three aquatic animal models commonly utilized in biomedical research, (Xiphophorus maculatus (platyfish), Oryzias latipes (medaka) and Danio rerio (zebrafish). Analysis of replicate comparative RNASeq data showed the transcriptional response to commonly utilized 4100K or "cool white" fluorescent light (FL) is much greater in platyfish and medaka than in zebrafish...
October 7, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
Manmohan Pandey, Ravindra Kumar, Prachi Srivastava, Suyash Agarwal, Shreya Srivastava, N S Nagpure, J K Jena, Basdeo Kushwaha
Mining and characterization of SSR markers from whole genomes provide valuable information about biological significance of SSR distribution and also facilitate development of markers for genetic analysis. WGS-SSR Annotation Tool (WGSSAT) is a graphical user interface pipeline developed using Java Netbeans and Perl scripts which facilitates in simplifying the process of SSR mining and characterization. WGSSAT takes input in FASTA format and automates the prediction of genes, ncRNA, core genes, repeats and SSRs from whole genomes followed by mapping of the predicted SSRs onto a genome (classified according to genes, ncRNA, repeats, exonic, intronic and core gene region) along with primer identification and mining of cross-species markers...
September 16, 2017: Journal of Heredity
Miguel González-Doncel, Salvador Sastre, Gregoria Carbonell, Eulalia María Beltrán, Cristina González Anaya, José Enrique García-Mauriño, Carlos Fernández Torija
A previous study conducted in our laboratory with growing medaka (Oryzias latipes) showed the capacity of BDE-47 (10-1000ng/g) to bioaccumulate during a 40-day oral exposure. However, the results did not provide evidence for effects during or after the exposure period. In this study, breeding medakas were fed a diet for 40days that contained 1000ng of BDE-47/g. At predefined time points, females (time points 10, 20, 30 and 40), males (time points 30 and 40) and pools of laid eggs (time points 10, 20, 30 and 40) were sampled and collected for: 1) the BDE-47 quantitative analysis in adults in the <24-h-old post-fertilization (hpf) embryos, and in the <24-h-old post-hatch (hph) eleutheroembryos; 2) the evaluation of fecundity, fertility and hatching...
September 28, 2017: Aquatic Toxicology
Maitrayee Chatterjee, Sharon D'Morris, Vinod Paul, Sruthi Warrier, Anil Kumar Vasudevan, Muralidharan Vanuopadath, Sudarslal Sadasivan Nair, Bindhu Paul-Prasanth, C Gopi Mohan, Raja Biswas
Pseudomonas aeruginosa depends on its quorum sensing (QS) system for its virulence factors' production and biofilm formation. Biofilms of P. aeruginosa on the surface of indwelling catheters are often resistant to antibiotic therapy. Alternative approaches that employ QS inhibitors alone or in combination with antibiotics are being developed to tackle P. aeruginosa infections. Here, we have studied the mechanism of action of 3-Phenyllactic acid (PLA), a QS inhibitory compound produced by Lactobacillus species, against P...
October 5, 2017: Applied Microbiology and Biotechnology
Ana Virginia Sánchez-Sánchez, Aránzazu Leal-Tassias, Neus Rodríguez-Sánchez, Marina Piquer-Gil, Patricia Martorell, Salvador Genovés, Carlos Acosta, Deborah Burks, Daniel Ramón, José Luis Mullor
Oxidative stress can induce cell apoptosis and thus, plays an important role in aging. Antioxidant foods protect tissues from oxidative stress and contribute to a healthier life style. Here we described the used of medaka embryos (Oryzias latipes) to study the putative antioxidant capacity of dietary cocoa extract in vertebrates. A polyphenol-enriched cocoa extract regulated the expression of several genes implicated in oxidative stress, thereby protecting fish embryos from induced oxidative stress. The cocoa extract activated SOD enzyme activity in embryos and in adult fish tissues, suggesting a common mechanism for protection during embryonic development and adulthood...
October 6, 2017: Rejuvenation Research
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