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Joaquín Letelier, Elisa de la Calle-Mustienes, Joyce Pieretti, Silvia Naranjo, Ignacio Maeso, Tetsuya Nakamura, Juan Pascual-Anaya, Neil H Shubin, Igor Schneider, Juan Ramón Martinez-Morales, José Luis Gómez-Skarmeta
Despite their evolutionary, developmental and functional importance, the origin of vertebrate paired appendages remains uncertain. In mice, a single enhancer termed ZRS is solely responsible for Shh expression in limbs. Here, zebrafish and mouse transgenic assays trace the functional equivalence of ZRS across the gnathostome phylogeny. CRISPR/Cas9-mediated deletion of the medaka (Oryzias latipes) ZRS and enhancer assays identify the existence of ZRS shadow enhancers in both teleost and human genomes. Deletion of both ZRS and shadow ZRS abolishes shh expression and completely truncates pectoral fin formation...
March 19, 2018: Nature Genetics
Lizhao Chen, Wei Zhang, Zhiqiang Guo, Li Zhang
Fish can acclimate to chronic arsenic (As) exposure, but the mechanisms of acclimation remain unclear to date. Therefore, this study conducted 28-d chronic inorganic As [As(III) and As(V)] exposures in freshwater medaka (Oryzias mekongensis), examined the As bioaccumulation and biotransformation during exposure, and the As acute toxicity and toxicokinetics after exposure. After chronic As(V) exposure, the 96-h lethal concentration (96-h LC50 ) of As(V) increased 1.3-fold (from 223 to 286 μmol/L), indicating that the fish became more tolerant to As(V)...
March 10, 2018: Environmental Pollution
J H Ryu, M S Kim, J H Kang, D H Kim, Y K Nam, S P Gong
This study was conducted to establish and characterize the clonal-cell lines from Siberian sturgeon Acipenser baerii head-kidney tissues and to evaluate its applicability as a research tool. From the culture of A. baerii head-kidney derived cells, 10 cell lines were established first and then eight clonal-cell lines were derived from clonal growth and colony expansion of two cell lines that showed significant high colony-forming ability. All eight clonal-cell lines were morphologically similar and grew stably under monolayer culture but their growth rates were significantly different...
March 12, 2018: Journal of Fish Biology
Mengjuan Shi, Cunli Zhang, Ivan Fan Xia, Siu To Cheung, Kwong Sen Wong, Ka-Hing Wong, Doris W T Au, David E Hinton, Kevin W H Kwok
Selenium (Se) is an essential element and its biological activity is related to its speciation. It is also well-known that in excess it can cause teratogenesis in fish and birds. In this study we compared dietary toxicity of elemental selenium nanoparticles (SeNPs) with selenite and selenomethionine (Se-Met). Japanese medaka (Oryzias latipes) was used as a laboratory model to determine Se effects on adults and their offspring. Adult females were individually exposed using a dry diet fortified with 0, 10 or 20 µg/g of the three Se species for 7 days and then allowed to breed for 3 days...
March 8, 2018: Ecotoxicology and Environmental Safety
Ahmed Abdel-Moneim, Cecon T Mahapatra, Maria S Sepúlveda
Estrogenic contaminants are commonly detected in water bodies and can result in reproductive effects of aquatic organisms. This necessitates the development of time and cost effective tools to screen chemicals for estrogenic potential. Our objective was to develop a novel in vivo visual reporter system for rapid detection of estrogenic contaminants. We developed a Japanese medaka (Oryzias latipes) transgenic line (pOSP1-AcGFP), which expresses Aequorea coerulescens green florescence protein (AcGFP) under the control of ovarian structural protein 1 (osp1) promoter region...
March 1, 2018: Chemosphere
Huafeng Zou, Zhaohui Lan, Mo Zhou, Weiqun Lu
The peptide urotensin II (UII) mediates multiple physiology effects in mammals and fishes, and UII expression showed a tissue-specific pattern. However the mechanism is still unknown. In the present study high level of UII mRNA was detected in the caudal neurosecretory system (CNSS) of the olive flounder when compared to other tissues. We examined whether epigenetic mechanisms of DNA methylation are involved in UII gene expression. Methylation DNA immune precipitation (MeDIP) assay showed low methylation of UII promoter in CNSS tissue compared with muscle and spinal cord...
March 6, 2018: General and Comparative Endocrinology
Benoît Sotton, Alain Paris, Séverine Le Manach, Alain Blond, Gérard Lacroix, Alexis Millot, Charlotte Duval, Hélène Huet, Qin Qiao, Sophie Labrut, Giovanni Chiappetta, Joelle Vinh, Arnaud Catherine, Benjamin Marie
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
March 6, 2018: Scientific Reports
Yusuke Inoue, Masahiko Kumagai, Xianbo Zhang, Tomonori Saga, Deshou Wang, Akihiko Koga, Hiroyuki Takeda
Background: Endogenous viral elements play important roles in eukaryotic evolution by giving rise to genetic novelties. Herpesviruses are a large family of DNA viruses, most of which do not have the ability to endogenize into host genomes. Recently, we identified a novel type of endogenous herpesvirus, which we named " Teratorn ", from the medaka ( Oryzias latipes ) genome, in which the herpesvirus is fused with a piggyBac -like DNA transposon, forming a novel mobile element...
2018: Zoological Letters
Tatsuya Sakamoto, Susumu Hyodo, Wataru Takagi
Corticosteroid receptors are critical for homeostasis maintenance, but understanding of the principal roles of the glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) throughout vertebrates is limited. Lines of constitutive GR-knockout zebrafish and MR-knockout medaka have recently been generated as the first adult-viable corticosteroid receptor-knockout animals, in contrast to the lethality of these receptor knockouts in mice. Here, we describe behavioral and physiological modifications following disruption of corticosteroid receptor function in these animal models...
February 23, 2018: Journal of Steroid Biochemistry and Molecular Biology
Shin-Ichi Chisada, Ayano Hirako, Akihiko Sugiyama
Ocular lesions in leptin receptor-deficient medaka were examined histopathologically at 10, 28, and 37 weeks post hatching. Leptin receptor-deficient medaka at 28 and 37 weeks old showed hyperglycemia and hypoinsulinemia. Histopathologically, vacuolation, swelling, fragmentation, and liquefaction of the lens fibers and dilatation of the retinal central veins, retinal capillaries, iridal veins and capillaries, and choroidal veins were observed in leptin receptor-deficient medaka at 28 and 37 weeks old. Thinning of the total retina, pigment epithelial layer, layer of rods and cones, outer granular layer, outer plexiform layer, inner granular layer, and inner plexiform layer was observed in leptin receptor-deficient medaka at 28 and 37 weeks compared with in control medaka...
January 2018: Journal of Toxicologic Pathology
Ahne Myklatun, Antonella Lauri, Stephan H K Eder, Michele Cappetta, Denis Shcherbakov, Wolfgang Wurst, Michael Winklhofer, Gil G Westmeyer
An impediment to a mechanistic understanding of how some species sense the geomagnetic field ("magnetoreception") is the lack of vertebrate genetic models that exhibit well-characterized magnetoreceptive behavior and are amenable to whole-brain analysis. We investigated the genetic model organisms zebrafish and medaka, whose young stages are transparent and optically accessible. In an unfamiliar environment, adult fish orient according to the directional change of a magnetic field even in darkness...
February 23, 2018: Nature Communications
Ferdaus Mohamat-Yusuff, Ab Ghafar Sarah-Nabila, Syaizwan Zahmir Zulkifli, Mohammad Noor Amal Azmai, Wan Norhamidah Wan Ibrahim, Shahrizad Yusof, Ahmad Ismail
This study was conducted to investigate the median lethal concentration (LC50) of copper pyrithione (CuPT) at 96-hr exposure on adult Javanese medaka (Oryzias javanicus) in revealing toxicological effects of CuPT contamination in the tropical area. Wild stock fishes were acclimatized for 14-days prior analysis. Triplicate of test tanks for seven test concentrations were placed with ten fishes each, this includes two control tanks. The behaviour of the tested fishes was manually observed through a camera. The LC50 of CuPT at 96-h was found to be 16...
February 2018: Marine Pollution Bulletin
Unni Grimholt
BACKGROUND: In sharks, chickens, rats, frogs, medaka and zebrafish there is haplotypic variation in MHC class I and closely linked genes involved in antigen processing, peptide translocation and peptide loading. At least in chicken, such MHCIa haplotypes of MHCIa, TAP2 and Tapasin are shown to influence the repertoire of pathogen epitopes being presented to CD8+ T-cells with subsequent effect on cell-mediated immune responses. RESULTS: Examining MHCI haplotype variation in Atlantic salmon using transcriptome and genome resources we found little evidence for polymorphism in antigen processing genes closely linked to the classical MHCIa genes...
February 23, 2018: BMC Evolutionary Biology
Hui-Su Kim, Bo-Young Lee, Jeonghoon Han, Chang-Bum Jeong, Dae-Sik Hwang, Min-Chul Lee, Hye-Min Kang, Duck-Hyun Kim, Daehwan Lee, Jaebum Kim, Ik-Young Choi, Jae-Seong Lee
Marine medaka (Oryzias melastigma) is considered to be a useful fish model for marine and estuarine ecotoxicology studies and has good potential for field-based population genomics because of its geographical distribution in Asian estuarine and coastal areas. In this study, we present the first whole genome draft of O. melastigma. The genome assembly consists of 8,602 scaffolds (N50=23.737 Mb) and a total genome length of 779.4 Mb. A total of 23,528 genes were predicted, and 12,670 gene families shared with three teleost species (Japanese medaka, mangrove killifish, and zebrafish) were identified...
February 16, 2018: Molecular Ecology Resources
Makiko Kamijo, Mayuko Kawamura, Shoji Fukamachi
Colour vision is often essential for animals. Fine discrimination of colours enhances the ability of animals to find food, predators, or mating partners. Using two colour variants of medaka (Oryzias latipes), which mate assortatively depending on visual cues (pale grey versus dark orange), we recently established red colour-blind strains by knocking out the red opsin (long-wavelength-sensitive) genes and elucidated that the fish were indeed insensitive to red light. In the present study, we investigated the mate choice of these red-blind fish...
February 12, 2018: Behavioural Processes
Ikuko Watakabe, Hisashi Hashimoto, Yukiko Kimura, Saori Yokoi, Kiyoshi Naruse, Shin-Ichi Higashijima
Background: Medaka ( Oryzias latipes ) is a popular animal model used in vertebrate genetic analysis. Recently, an efficient (~ 30%) knock-in system via non-homologous end joining (NHEJ) was established in zebrafish using the CRISPR/Cas9 system. If the same technique were applicable in medaka, it would greatly expand the usefulness of this model organism. The question of the applicability of CRISPR/Cas9 in medaka, however, has yet to be addressed. Results: We report the highly efficient generation of knock-in transgenic medaka via non-homologous end joining (NHEJ)...
2018: Zoological Letters
Kota Abe, Toru Kawanishi, Hiroyuki Takeda
The medaka contains seven zic genes, two of which, zic1 and zic4, have been studied extensively. The analyses are mainly based on the double anal fin (Da) mutant, which was isolated from the wild. Da is an enhancer mutant of zic1/zic4, and the expression of zic1/zic4 is specifically lost in the dorsal half of the somites, which leads to a mirror-image duplication of the ventral half across the lateral midline from larva to adult. The studies of medaka Da give us important insights into the function of zic1/zic4 in mesodermal tissues and also the mechanism of dorsoventral patterning in the vertebrate trunk region occurring during late development, which is a long-standing mystery in developmental biology...
2018: Advances in Experimental Medicine and Biology
Chie Umatani, Yoshitaka Oka
Peptidergic neurons are suggested to play a key role in neuromodulation of animal behaviors in response to sensory cues in the environment. Terminal nerve gonadotropin releasing hormone 3 (TN-GnRH3) neurons are thought to be one of the peptidergic neurons important for such neuromodulation in adult vertebrates. On the other hand, it has been reported that TN-GnRH3 neurons are labeled by specific GnRH3 antibody from early developmental stages to adulthood and are thus suggested to produce mature GnRH3 peptide even in the early developmental stages...
February 1, 2018: Endocrinology
Xianliang Yi, Changlin Li, Xi Zhong, Yufeng Gong
A lipovitellin (Lv) based sandwich enzyme-linked immunosorbent assay (ELISA) was developed to quantify vitellogenin (Vtg) in marine medaka (Oryzias melastigma). Lv and Vtg were purified from the unfertilized eggs and the whole body homogenates (WBH) of estradiol (E2)-exposed fish. The purified Lv sample appeared as three clear bands (118, 112 and 100 kDa) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and was identified as an Lvs mixture from VtgAa and VtgAb by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis...
January 22, 2018: Chemosphere
Xiaocui Dong, Lemeng Zhang, Meng Chen, Zhibin Yang, Zhenghong Zuo, Chonggang Wang
Difenoconazole (DFZ) is a triazole fungicide which has been detected in the aquatic environment, including estuaries and embayments. However, few studies addressing the reproductive toxicity and transgenerational effects of DFZ on marine fishes are available. The present study was conducted to investigate the effects of DFZ on male marine medaka (Oryzias melastigma). After exposure of the embryo to 1, 10, 100 and 1000ng/L DFZ for 180days, the gonadosomatic index was significantly decreased in the 1000ng/L treatment...
January 2018: Journal of Environmental Sciences (China)
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