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https://www.readbyqxmd.com/read/28110168/ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry-uhplc-ms-ms-for-determination-of-ghb-precursors-and-metabolites-in-different-specimens-application-to-clinical-and-forensic-cases
#1
Francesco Paolo Busardò, Chrystalla Kyriakou, Emilia Marchei, Roberta Pacifici, Daniel Sejer Pedersen, Simona Pichini
Gamma-hydroxybutyric acid (GHB) acts as a precursor and metabolite of the inhibitory central nervous system (CNS) neurotransmitter gamma-aminobutyric acid (GABA). Sodium salt of GHB has been used as a medication for narcolepsy and alcohol withdrawal. Moreover, GHB and its precursor gamma-butyrolactone (GBL), are illegal recreational drugs of abuse. A procedure based on ultra-high-performance liquid chromatography tandem mass spectrometry has been developed and validated in plasma, urine, cerebrospinal fluid and hair for acute and chronic exposure to GHB and in seized preparations coming from black market...
January 9, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28110022/multielemental-analysis-of-some-soils-in-karaman-by-paa-using-a-clinac
#2
Osman Agar, Ismail Boztosun, Christian Segebade
In this analytical work, some components (Sr, Ni, Mg, Fe, Zr, Cd, As, Ca, Br, Rb, Mn, I, Zn, Pb, Tl, U and Ba) of soil samples collected in different locations in the surroundings of Karaman, Turkey were determined by photon activation analysis (PAA). This method is based on photonuclear reactions. (γ,n), (γ,2n), (γ,γ') and (γ,p)-type reactions have been observed. The samples were irradiated with bremsstrahlung photons produced by a clinical electron linear accelerator (cLINAC) for up to about 85min. The PAA results were compared with those obtained by X-ray fluorescence (XRF)...
January 15, 2017: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/28109243/neonatal-hyperbilirubinemia-in-a-marginalized-population-on-the-thai-myanmar-border-a-study-protocol
#3
Laurence Thielemans, Margreet Trip-Hoving, Germana Bancone, Claudia Turner, Julie A Simpson, Borimas Hanboonkunupakarn, Michaël Boele van Hensbroek, Patrick van Rheenen, Moo Kho Paw, François Nosten, Rose McGready, Verena I Carrara
BACKGROUND: This study aims to identify risk factors and the neurodevelopmental impact of neonatal hyperbilirubinemia in a limited-resource setting among a refugee and migrant population residing along the Thai-Myanmar border, an area with a high prevalence of glucose-6-phosphate dehydrogenase-deficiency. METHODS: This is an analytic, observational, prospective birth cohort study including all infants of estimated gestational age equal to or greater than 28 weeks from mothers who followed antenatal care in the Shoklo Malaria Research Unit clinics...
January 21, 2017: BMC Pediatrics
https://www.readbyqxmd.com/read/28109054/intracoronary-optical-coherence-tomography-clinical-and-research-applications-and-intravascular-imaging-software-overview
#4
Erhan Tenekecioglu, Felipe N Albuquerque, Yohei Sotomi, Yaping Zeng, Pannipa Suwannasom, Hiroki Tateishi, Rafael Cavalcante, Yuki Ishibashi, Shimpei Nakatani, Mohammad Abdelghani, Jouke Dijkstra, Christos Bourantas, Carlos Collet, Antonios Karanasos, Maria Radu, Ancong Wang, Takashi Muramatsu, Ulf Landmesser, Takayuki Okamura, Evelyn Regar, Lorenz Räber, Giulio Guagliumi, Robert T Pyo, Yoshinobu Onuma, Patrick W Serruys
By providing valuable information about the coronary artery wall and lumen, intravascular imaging may aid in optimizing interventional procedure results and thereby could improve clinical outcomes following percutaneous coronary intervention (PCI). Intravascular optical coherence tomography (OCT) is a light-based technology with a tissue penetration of approximately 1 to 3 mm and provides near histological resolution. It has emerged as a technological breakthrough in intravascular imaging with multiple clinical and research applications...
January 21, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/28109032/comparison-of-methods-and-tat-assessment-volumetric-aquios-cl-and-bead-based-facs-canto-ii-cytometers
#5
Valentina Grossi, Maria Infantino, Francesca Meacci, Emanuele Bellio, Valerio Bellio, Giovanna Ciotta, Fiorella Priami, Piercarlo Sarzi-Puttini, Fabiola Atzeni, Francesca Li Gobbi, Arianna Damiani, Maurizio Benucci, Mariangela Manfredi
BACKGROUND: Measurement of lymphocyte subpopulations is a crucial parameter in the diagnosis and monitoring of therapy in a wide variety of clinical conditions. We compared different flow cytometry-based methods to determine lymphocyte subsets counts of routine samples by volumetric AQUIOS CL (Beckman Coulter) and bead-based FACS CANTO II (BD Biosciences) cytometers.We evaluated the possible decrease of the labor intensive technical work using the fully automated AQUIOS CL system in the pre and post analytical steps in comparison with our routine flow cytometer FACS CANTO II, toward the reduction of laboratory analytical turnaround time (TAT)...
January 20, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28107923/a-sensitive-and-rapid-uflc-apci-ms-ms-bioanalytical-method-for-quantification-of-endogenous-and-exogenous-vitamin-k1-isomers-in-human-plasma-development-validation-and-first-application-to-a-pharmacokinetic-study
#6
Nagaraj Kumar Nannapaneni, Sunil S Jalalpure, Rajendraprasad Muppavarapu, Sunil Kumar Sirigiri
Due to lack of suitable bioanalytical methods in previous literature, for simultaneous estimation of Vitamin K1 isomers, in compliance with the current regulatory expectation, we aimed to develop a sensitive and rapid method with UFLC-APCI-MS/MS (ultrafast liquid chromatography - tandem mass spectrometry) using human plasma. A simple and cost effective procedure was implemented with the combination of protein precipitation and liquid extraction, to isolate the targets from plasma sample, while achieving an insignificant matrix effects and high recovery (≥88...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107906/development-of-data-independent-acquisition-workflows-for-metabolomic-analysis-on-a-quadrupole-orbitrap-platform
#7
Juntuo Zhou, Yuhua Li, Xi Chen, Lijun Zhong, Yuxin Yin
Untargeted metabolomic profiling has been widely used in recent years. However, the low reproducibility of the data-dependent acquisition (DDA) strategy presents a major bottleneck that considerably limits the reliability of metabolomic studies in biological and clinical research. The data-independent acquisition (DIA) strategy is proposed to solve the above-mentioned problem, and it is gaining popularity. This paper presents a novel approach for performing metabolomic analysis using an untargeted, liquid chromatography-data independent-mass spectrometry (LC-DIA-MS) strategy on a quadrupole-Orbitrap platform...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107832/a-simple-method-to-produce-2d-and-3d-microfluidic-paper-based-analytical-devices-for-clinical-analysis
#8
Ricardo A G de Oliveira, Fiamma Camargo, Naira C Pesquero, Ronaldo Censi Faria
This paper describes the fabrication of 2D and 3D microfluidic paper-based analytical devices (μPADs) for monitoring glucose, total protein, and nitrite in blood serum and artificial urine. A new method of cutting and sealing filter paper to construct μPADs was demonstrated. Using an inexpensive home cutter printer soft cellulose-based filter paper was easily and precisely cut to produce pattern hydrophilic microchannels. 2D and 3D μPADs were designed with three detection zones each for the colorimetric detection of the analytes...
March 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28107395/prevalence-of-pre-analytical-errors-in-clinical-chemistry-diagnostic-labs-in-sulaimani-city-of-iraqi-kurdistan
#9
Dereen Najat
BACKGROUND: Laboratory testing is roughly divided into three phases: a pre-analytical phase, an analytical phase and a post-analytical phase. Most analytical errors have been attributed to the analytical phase. However, recent studies have shown that up to 70% of analytical errors reflect the pre-analytical phase. The pre-analytical phase comprises all processes from the time a laboratory request is made by a physician until the specimen is analyzed at the lab. Generally, the pre-analytical phase includes patient preparation, specimen transportation, specimen collection and storage...
2017: PloS One
https://www.readbyqxmd.com/read/28107166/a-new-strategy-for-calculating-the-risk-of-ovarian-malignancy-algorithm-roma
#10
Tae-Dong Jeong, Eun-Jung Cho, Dae-Hyun Ko, Woochang Lee, Sail Chun, Hi Jeong Kwon, Ki-Sook Hong, Yong-Man Kim, Won-Ki Min
BACKGROUND: Reliable quantitative measurements of HE4 and CA125 levels are required to calculate the risk of ovarian malignancy algorithm (ROMA) value. We suggest a new reporting strategy for interpreting ROMA values based on analytical measurement range (AMR) and qualified-intervals of the HE4 and CA125 results. METHODS: HE4 and CA125 assays from Abbott and Roche were used. The AMRs and the qualified-intervals were as follows: Architect HE4 assay, 20-1500 and 17...
January 20, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28107066/hematologic-plasma-biochemical-and-lipid-panel-reference-intervals-in-orange-winged-amazon-parrots-amazona-amazonica
#11
Claire Vergneau-Grosset, Tamsen Polley, Danielle Carrade Holt, William Vernau, Joanne Paul-Murphy
To establish reference intervals in orange-winged Amazon parrots ( Amazona amazonica ) for the complete blood count, plasma biochemical values, and lipid panel and to evaluate age- and sex-related variations, blood samples were obtained from 29 healthy juvenile and adult parrots. Concentrations of total protein, bile acids, phosphorus, total cholesterol, low-density-lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol were significantly higher in adult compared with juvenile birds, while uric acid concentration was significantly higher in juveniles...
December 2016: Journal of Avian Medicine and Surgery
https://www.readbyqxmd.com/read/28106507/association-between-anxiety-and-pain-in-dental-treatment
#12
C-S Lin, S-Y Wu, C-A Yi
Accumulating evidence has revealed that dental anxiety (DA), as a dispositional factor toward the dental situation, is associated with the state anxiety (SA) and pain related to dental procedures. However, conclusions from individual studies may be limited by the treatment procedures that patients received, the tools used to assess DA, or the treatment stages when anxiety or pain was assessed. It is unclear whether DA, at the study level, accounts for the variance in pretreatment SA. The impact of DA and SA on pain at different treatment stages has not been systematically investigated...
February 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28106173/hykl-40-cancer-biomarker-electroanalysis-in-serum-samples-and-model-cell-lysates-capacitive-immunosensing-compared-with-enzyme-label-immunosorbent-assays-elisa
#13
W Chaocharoen, A Schulte, W Suginta
Human chitinase 3-like protein 1 (CHI3L1 or hYKL-40), a potential molecular marker for several cancers, was measured in clinical human serum samples and model cell lysates by indirect and competitive enzyme-linked immunosorbent assay (ELISA) and by capacitive immunosensing, so as to evaluate a recently introduced electrochemical method for routine use in cancer-monitoring studies. The clinical samples tested included serum from four healthy individuals, five breast cancer and four glioblastoma patients; cultures of the human monocytic cell line THP-1, known to secrete hYKL-40, and of the human embryonic kidney 293t cell line, which does not express hYKL-40, provided cell lysates and cell culture media for positive and negative bio- and electrochemical control trials...
January 20, 2017: Analyst
https://www.readbyqxmd.com/read/28105837/hrp-mimicking-dnazyme-catalyzed-in-situ-generation-of-polyaniline-to-assist-signal-amplification-for-ultrasensitive-surface-plasmon-resonance-biosensing
#14
Haiyin Li, Jiafu Chang, Ting Hou, Feng Li
It is well-known that the horseradish peroxidase- (HRP-) mimicking DNAzyme, namely, hemin/G-quadruplex, can effectively catalyze the polymerization of aniline to form DNA-guided polyaniline. Meanwhile, polyaniline exhibits extraordinary electrical, electrochemical, and redox properties, as well as excellent SPR signal-enhancing ability. Herein, we report a novel ultrasensitive surface plasmon resonance (SPR) biosensor based on HRP-mimicking DNAzyme-catalyzed in situ formation of polyaniline for signal amplification, using bleomycin (BLM) as the proof-of-concept analyte...
January 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28104926/the-bending-stress-distribution-in-bilayered-and-graded-zirconia-based-dental-ceramics
#15
Douglas Fabris, Júlio C M Souza, Filipe S Silva, Márcio Fredel, Joana Mesquita-Guimarães, Yu Zhang, Bruno Henriques
The purpose of this study was to evaluate the biaxial flexural stresses in classic bilayered and in graded zirconia-feldspathic porcelain composites. A finite element method and an analytical model were used to simulate the piston-on-ring test and to predict the biaxial stress distributions across the thickness of the bilayer and graded zirconia-feldspathic porcelain discs. An axisymmetric model and a flexure formula of Hsueh et al. were used in the FEM and analytical analysis, respectively. Four porcelain thicknesses were tested in the bilayered discs...
July 2016: Ceramics International
https://www.readbyqxmd.com/read/28104580/leveraging-electronic-health-care-record-information-to-measure-pressure-ulcer-risk-in-veterans-with-spinal-cord-injury-a-longitudinal-study-protocol
#16
Stephen L Luther, Susan S Thomason, Sunil Sabharwal, Dezon K Finch, James McCart, Peter Toyinbo, Lina Bouayad, Michael E Matheny, Glenn T Gobbel, Gail Powell-Cope
BACKGROUND: Pressure ulcers (PrUs) are a frequent, serious, and costly complication for veterans with spinal cord injury (SCI). The health care team should periodically identify PrU risk, although there is no tool in the literature that has been found to be reliable, valid, and sensitive enough to assess risk in this vulnerable population. OBJECTIVE: The immediate goal is to develop a risk assessment model that validly estimates the probability of developing a PrU...
January 19, 2017: JMIR Research Protocols
https://www.readbyqxmd.com/read/28103883/performance-of-loop-mediated-isothermal-amplification-lamp-for-the-diagnosis-of-malaria-among-malaria-suspected-pregnant-women-in-northwest-ethiopia
#17
Banchamlak Tegegne, Sisay Getie, Wossenseged Lemma, Abu Naser Mohon, Dylan R Pillai
BACKGROUND: Malaria is a major public health problem and an important cause of maternal and infant morbidity in sub-Saharan Africa, including Ethiopia. Early and accurate diagnosis of malaria with effective treatment is the best strategy for prevention and control of complications during pregnancy and infant morbidity and mortality. However, laboratory diagnosis has relied on the identification of malaria parasites and parasite antigens in peripheral blood using Giemsa-stained microscopy or rapid diagnostic tests (RDTs) which lack analytical and clinical sensitivity...
January 19, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28103450/paper-based-analytical-devices-for-clinical-diagnosis-recent-advances-in-the-fabrication-techniques-and-sensing-mechanisms
#18
Mazhar Sher, Rachel Zhuang, Utkan Demirci, Waseem Asghar
There is a significant interest in developing inexpensive portable biosensing platforms for various applications including disease diagnostics, environmental monitoring, food safety, and water testing at the point-of-care (POC) settings. Current diagnostic assays available in the developed world require sophisticated laboratory infrastructure and expensive reagents. Hence, they are not suitable for resource-constrained settings with limited financial resources, basic health infrastructure, and few trained technicians...
January 19, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28102954/dosimetric-impact-of-lipiodol-in-stereotactic-body-radiation-therapy-on-liver-after-trans-arterial-chemoembolization
#19
Daisuke Kawahara, Shuichi Ozawa, Akito Saito, Teiji Nishio, Tomoki Kimura, Tatsuhiko Suzuki, Kazunari Hioki, Takeo Nakashima, Yoshimi Ohno, Yuji Murakami, Yasushi Nagata
PURPOSE: Stereotactic body radiation therapy (SBRT) combining trans-arterial chemoembolization (TACE) with Lipiodol is expected to improve local control. This study is aimed to estimate the dose enhancement in Lipiodol's proximity and to evaluate the dose calculation accuracy of the Acuros XB (AXB) algorithm and anisotropic analytical algorithm (AAA) in the Eclipse treatment planning system (TPS) (ver. 11, Varian Medical Systems, Palo Alto, USA), compared with that of the Monte Carlo (MC) calculation (using BEAMnrc/DOSXYZnrc code) for a virtual phantom and a treatment plan for liver SBRT after TACE...
January 2017: Medical Physics
https://www.readbyqxmd.com/read/28102570/simultaneous-determination-of-free-and-total-prostate-specific-antigen-by-a-magnetic-particle-based-time-resolved-fluoroimmunoassay
#20
Bin Zhou, Jue Zhang, Zhongwei Lv, Jun Fan, Yi Zhang, Biao Huang
OBJECTIVES: In this paper, a novel, sensitive and rapid method to simultaneously determine the free and total prostate-specific antigen (fPSA and tPSA) in serum by combining a time-resolved fluoroimmunoassay (TRFIA) and immunomagnetic separation was described. METHODS: The new approach uses magnetic particles as an immobilization matrix and means of separation, whereas the luminescent europium (Eu) and samarium (Sm) chelates are used as probes. The proposed method was evaluated via a single-step, sandwich-type TRFIA immunoassay of tPSA and fPSA as model analytes in serum...
January 19, 2017: Journal of Clinical Laboratory Analysis
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