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Gerald Klaassen, Dorien M Zelle, Gerjan J Navis, Desie Dijkema, Frederike J Bemelman, Stephan J L Bakker, Eva Corpeleijn
BACKGROUND: Low physical activity and reduced physical functioning are common after renal transplantation, resulting in a reduced quality of life. Another common post-transplantation complication is poor cardio-metabolic health, which plays a main role in long-term outcomes in renal transplant recipients (RTR). It is increasingly recognized that weight gain in the first year after transplantation, especially an increase in fat mass, is a highly common contributor to cardio-metabolic risk...
September 15, 2017: BMC Nephrology
Olivier Désy, Stéphanie Béland, Patrice Vallin, Julie Riopel, Eva Latulippe, Nader Najafian, Anil Chandraker, Mohsen Agharazii, Ibrahim Batal, Sacha A De Serres
Although borderline changes (BL) suspicious for acute T-cell-mediated rejection represents a diagnostic category its clinical relevance is questioned leading to heterogeneous therapeutic management. We hypothesized that measuring IL-6 secretion by peripheral blood mononuclear cells identifies patients with ongoing graft damage. We examined the association between secreted IL-6 and the change in estimated glomerular filtration rate at 6 months after the biopsy (ΔeGFR). We then conducted phenotypic and functional studies on patient and mouse innate immune cells in the blood and the kidney...
September 14, 2017: Transplant International: Official Journal of the European Society for Organ Transplantation
Stephen Ambrose, Nicholas G Housden, Kallol Gupta, Jieyuan Fan, Paul White, Hsin-Yung Yen, Julien Marcoux, Colin Kleanthous, Jonathan T S Hopper, Carol V Robinson
Mass spectrometry (MS) applications for intact protein complexes typically require electrospray (ES) ionization and have not been achieved via direct desorption from surfaces. Desorption ES ionization (DESI) MS has however transformed the study of tissue surfaces through release and characterisation of small molecules. Motivated by the desire to screen for ligand binding to intact protein complexes we report the development of a native DESI platform. By establishing conditions that preserve non-covalent interactions we exploit the surface to capture a rapid turnover enzyme-substrate complex and to optimise detergents for membrane protein study...
September 8, 2017: Angewandte Chemie
Keaton S Nahan, Noe Alvarez, Vesselin Shanov, Anne Vonderheide
Mass spectrometry continues to tackle many complicated tasks, and ongoing research seeks to simplify its instrumentation as well as sampling. The desorption electrospray ionization (DESI) source was the first ambient ionization source to function without extensive gas requirements and chromatography. Electrospray techniques generally have low efficiency for ionization of nonpolar analytes and some researchers have resorted to methods such as direct analysis in real time (DART) or desorption atmospheric pressure chemical ionization (DAPCI) for their analysis...
September 7, 2017: Journal of the American Society for Mass Spectrometry
Lucilia Kato, Aline Pereira Moraes, Cecília Maria Alves de Oliveira, Boniek Gontijo Vaz, Letícia de Almeida Gonçalves, Elienai Cândida E Silva, Christian Janfelt
INTRODUCTION: Species of the genera Psychotria and Palicourea are sources of indole alkaloids, however, the distribution of alkaloids within the plants is not known. Analysing the spatial distribution using desorption electrospray ionisation mass spectrometry imaging (DESI-MSI) has become attractive due to its simplicity and high selectivity compared to traditional histochemical techniques. OBJECTIVES: To apply DESI-MSI to visualise the alkaloid distribution on the leaf surface of Psychotria prunifolia and Palicourea coriacea and to compare the distributions with HPLC-MS and histochemical analyses...
September 6, 2017: Phytochemical Analysis: PCA
Sonika Pandey, Aprajita Kumari, Kapuganti Jagadis Gupta
Chickpea is an important leguminous crop that belongs to Fabaceae family, highly valued for its nutritious seeds. Seeds contain reserve food for the developing embryos. Mitochondria are crucial organelle for generation of chemical energy in the form of ATP which is required for achieving metabolically active state; therefore, investigating mitochondrial function and respiration rate is crucial for exploring various metabolic and physio-biochemical changes that occur during seed germination. Here we describe a method for isolation of mitochondria from germinating seeds of two chickpea varieties, i...
2017: Methods in Molecular Biology
Nagayoshi Asano, Justin Michael Wiseman, Fumio Tsuji, Kouichi Kawazu
Meibomian gland dysfunction (MGD) is the leading cause of dry eye, and although it affects approximately 4% of the population, treatment options remain limited. Topical azithromycin is one of the most promising pharmacological agents because of its multiple mechanisms of action and long sustainability. Azithromycin is frequently used as an off-label medication in the U.S. However, although azithromycin is presumed to act directly on meibomian gland cells, the mechanisms of action that contribute to its clinical efficacy remain unclear because no studies using a pharmacokinetic approach have been performed...
2017: Biological & Pharmaceutical Bulletin
Michael Woolman, Alessandra Tata, Delaram Dara, Jalna Meens, Elisa D'Arcangelo, Consuelo J Perez, Shamina Saiyara Prova, Emma Bluemke, Howard J Ginsberg, Demian Ifa, Alison McGuigan, Laurie Ailles, Arash Zarrine-Afsar
Correction for 'Rapid determination of the tumour stroma ratio in squamous cell carcinomas with desorption electrospray ionization mass spectrometry (DESI-MS): a proof-of-concept demonstration' by Michael Woolman et al., Analyst, 2017, 142, 3250-3260.
September 1, 2017: Analyst
Alexandra Kesler Johnson, Natalie Lynch, Desi Newberry, Amy J Jnah
BACKGROUND: Diuretics are among the most frequently prescribed medications in the neonatal intensive care unit (NICU), despite minimal data regarding the safety and efficacy of their use in the neonatal population. Off-label diuretic therapy is used in preterm and full-term infants to both optimize kidney function and improve respiratory status. PURPOSE: This article examines the literature specific to the impact of diuretic therapy in the NICU and compares the benefits versus risks of utilization as they pertain to the prevention and treatment of renal and pulmonary dysfunction in this population...
October 2017: Advances in Neonatal Care: Official Journal of the National Association of Neonatal Nurses
Zheng Wang, Haijing Zhu, Guangming Huang
RATIONALE: Although desorption electrospray ionization (DESI) is claimed to have less ion suppression effect than electrospray ionization (ESI), related investigation with quantification measurement of ion suppression effect is absent. Herein, comparative analysis of ion suppression effect between DESI and ESI was conducted, aiming to quantitatively study the ion suppression effect. METHODS: CTVA mixtures, constant concentration of tioconazole with varied concentrations of atenolol, were detected for ESI and DESI...
August 31, 2017: Rapid Communications in Mass Spectrometry: RCM
Vandra C Harris, Anne R Links, Paul Hong, Jonathan Walsh, Desi P Schoo, David E Tunkel, Charles M Stewart, Emily F Boss
OBJECTIVE: Tympanostomy tube (TT) placement is common in children; however, family-centeredness and utility of online information used for decision making and understanding is unknown. We evaluate the quality of leading Internet resources describing TT placement. STUDY DESIGN: Cross-sectional descriptive design. METHODS: We performed a Google (Menlo Park, CA) search for terms related to TTs. We defined quality using scaled readability measures (Flesch Reading Ease and Flesch-Kincaid Grade-Level), understandability and actionability (Patient Education Materials Assessment Tool), shared decision-making centrality (Center for Medicare and Medicaid Services informed consent guidelines), and clinical practice guideline (CPG) compatibility...
August 26, 2017: Laryngoscope
Hilde-Marléne Bergman, Ingela Lanekoff
Molecular profiling of single cells has the potential to significantly advance our understanding of cell function and cellular processes of importance to health and disease. In particular, small molecules with rapid turn-over rates can reveal activated metabolic pathways resulting from an altered chemical environment or cellular events such as differentiation. Consequently, techniques for quantitative metabolite detection acquired in a higher throughput manner are needed to characterize the biological variability between seemingly homogenous cells...
October 7, 2017: Analyst
Kyle D Duncan, Hilde-Marléne Bergman, Ingela Lanekoff
Nanospray desorption electrospray ionization (nano-DESI) has been established as a powerful technique for mass spectrometry imaging (MSI) of biomolecules from tissue samples. The direct liquid extraction of analytes from a surface at ambient pressure negates the need for significant sample preparation or matrix application. Although many recent studies have applied nano-DESI to new and exciting applications, there has not been much work in the development and improvement of the nano-DESI source. Here, we incorporate a nebulizer to replace the self-aspirating secondary capillary in the conventional nano-DESI setup, and characterize the device by use of rat kidney tissue sections...
August 22, 2017: Analyst
Joanna P MacEwan, Katharine Batt, Wes Yin, Desi Peneva, Steve Sison, Seanna Vine, Clara Chen
This study characterized the costs of multiple myeloma (MM) during first-line (1L), second-line (2L) and third-line (3L) treatment from the US payer perspective. Patients with ≥2 outpatient or ≥1 inpatient claims with a primary MM diagnosis and 12 months continuous enrollment post index were identified in a retrospective claims database between 1 July 2006 and 30 June 2013. A cost per-patient per-month (PPPM) metric was used to calculate total all-cause and anti-MM pharmacy costs in 1L, 2L, and 3L treatment...
August 13, 2017: Leukemia & Lymphoma
Michael Woolman, Alessandra Tata, Delaram Dara, Jalna Meens, Elisa D'Arcangelo, Consuelo J Perez, Shamina Saiyara Prova, Emma Bluemke, Howard J Ginsberg, Demian Ifa, Alison McGuigan, Laurie Ailles, Arash Zarrine-Afsar
Squamous cell carcinomas constitute a major class of head & neck cancers, where the tumour stroma ratio (TSR) carries prognostic information. Patients affected by stroma-rich tumours exhibit a poor prognosis and a higher chance of relapse. As such, there is a need for a technology platform that allows rapid determination of the tumour stroma ratio. In this work, we provide a proof-of-principle demonstration that Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) can be used to determine tumour stroma ratios...
August 11, 2017: Analyst
Chaevien S Clendinen, María Eugenia Monge, Facundo M Fernández
Since the introduction of desorption electrospray ionization (DESI) mass spectrometry (MS), ambient MS methods have seen increased use in a variety of fields from health to food science. Increasing its popularity in metabolomics, ambient MS offers limited sample preparation, rapid and direct analysis of liquids, solids, and gases, in situ and in vivo analysis, and imaging. The metabolome consists of a constantly changing collection of small (<1.5 kDa) molecules. These include endogenous molecules that are part of primary metabolism pathways, secondary metabolites with specific functions such as signaling, chemicals incorporated in the diet or resulting from environmental exposures, and metabolites associated with the microbiome...
August 9, 2017: Analyst
Brittany Watson Frock, Amy J Jnah, Desi M Newberry
Tricuspid atresia (TA) is a rare congenital heart defect in which the right atrioventricular connection, the tricuspid valve, is absent. As a result, there is no direct communication between the right atrium and right ventricle. Surgical treatment, including the Fontan procedure, is indicated yet palliative, leaving patients with various lifelong complications. A comprehensive literature review revealed a paucity of evidence-based education on the identification, evaluation, management, treatment, and life span implications of TA...
July 1, 2017: Neonatal Network: NN
Lucia Mainero Rocca, Juri Cecca, Nunziata L'Episcopo, Giovanni Fabrizi
A new field of application for a relatively new mass-spectrometric interface such as desorption electrospray ionization was evaluated. For this purpose, its behavior was tested versus quantitative analysis of dimethoate, trifloxystrobin and tebuconazole directly on olive and vine leaves surface. The goal was workers exposure assessment during field re-entry operations since evidence suggests an association between chronic occupational exposure to some agrochemicals and severe adverse effects. DESI gave good response working in positive ionization mode, while numerous test were necessary for the choice of a unique blend of spray solvents suitable for all three substances...
August 1, 2017: Journal of Mass Spectrometry: JMS
Wan-Ning Yu, Desi Hanna Natalia Manik, Chun-Jen Huang, Lai-Kwan Chau
Carboxybetaine (CB)-based zwitterionic materials have attracted considerable attention due to their dual antifouling and functionalizable properties. In this communication, the elimination effect on the antifouling and pH-responsive properties of CB materials was investigated. We synthesize β- and α-substituted methyl CB materials to investigate the occurrence of elimination in the ethylene intercharge arm in a harsh basic solution. This work provides molecular understanding of a structure-property relationship of the CB moiety for material development...
August 1, 2017: Chemical Communications: Chem Comm
Janice L Farlow, Benjamin Ulrich, Desi Schiess, Andrew Piropato, Avinash Inabathula, Nathanael Moore, Michelle Chung, Antwione Haywood
No abstract text is available yet for this article.
July 31, 2017: Medical Teacher
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