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https://www.readbyqxmd.com/read/28211480/visualisation-of-abscisic-acid-and-12-oxo-phytodienoic-acid-in-immature-phaseolus-vulgaris-l-seeds-using-desorption-electrospray-ionisation-imaging-mass-spectrometry
#1
Hirofumi Enomoto, Takuya Sensu, Kei Sato, Futoshi Sato, Thanai Paxton, Emi Yumoto, Koji Miyamoto, Masashi Asahina, Takao Yokota, Hisakazu Yamane
The plant hormone abscisic acid (ABA) and the jasmonic acid related-compound 12-oxo-phytodienoic acid (OPDA) play crucial roles in seed development, dormancy, and germination. However, a lack of suitable techniques for visualising plant hormones has restricted the investigation of their biological mechanisms. In the present study, desorption electrospray ionisation-imaging mass spectrometry (DESI-IMS), a powerful tool for visualising metabolites in biological tissues, was used to visualise ABA and OPDA in immature Phaseolus vulgaris L...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28208268/medical-swab-analysis-using-desorption-electrospray-ionization-mass-spectrometry-a-noninvasive-approach-for-mucosal-diagnostics
#2
Pamela Pruski, David A MacIntyre, Holly V Lewis, Paolo Inglese, Gonçalo D S Correia, Trevor T Hansel, Phillip R Bennett, Elaine Holmes, Zoltan Takats
Medical swabs are routinely used worldwide to sample human mucosa for microbiological screening with culture methods. These are usually time-consuming and have a narrow focus on screening for particular microorganism species. As an alternative, direct mass spectrometric profiling of the mucosal metabolome provides a broader window into the mucosal ecosystem. We present for the first time a minimal effort/minimal-disruption technique for augmenting the information obtained from clinical swab analysis with mucosal metabolome profiling using desorption electrospray ionization mass spectrometry (DESI-MS) analysis...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28202379/comprehensive-preoperative-work-up-and-surgical-treatment-of-low-grade-tumor-benign-lesion-related-temporal-lobe-epilepsy
#3
Rui Feng, Jie Hu, Jinsong Wu, Liqin Lang, Chengxin Ma, Shize Jiang, Bing Sun, Xin Gu, Li Pan
OBJECTIVE: Generally low-grade tumor/benign lesion related temporal lobe epilepsy (LGT/BL-TLE) is considered easier to treat and has better prognosis when compared to non-lesional TLE. However, multiple disputes exist in surgical management of this epilepsy entity. This study aims to discuss comprehensive preoperative work-up, surgical strategies and outcome of it. METHODS: A retrospective review of sixty LGT/BL-TLE cases which underwent comprehensive preoperative work-up and then resective surgeries was conducted...
February 12, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/28194988/personal-information-from-latent-fingerprints-using-desorption-electrospray-ionization-mass-spectrometry-and-machine-learning
#4
Zhenpeng Zhou, Richard N Zare
Desorption electrospray ionization-mass spectrometry imaging (DESI-MSI) was applied to latent fingerprints to obtain not only spatial patterns but also chemical maps. Samples with similar lipid compositions as those of the fingerprints were collected by swiping a glass slide across the forehead of consenting adults. A machine learning model called gradient boosting tree ensemble (GDBT) was applied to the samples that allowed us to distinguish between different genders, ethnicities, and ages (within 10 years)...
January 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28193010/variations-in-the-abundance-of-lipid-biomarker-ions-in-mass-spectrometry-images-correlate-to-tissue-density
#5
Jade Bilkey, Alessandra Tata, Trevor D McKee, Andreia M Porcari, Emma Bluemke, Michael Woolman, Manuela Ventura, Marcos N Eberlin, Arash Zarrine-Afsar
While mass spectrometry (MS) imaging is widely used to investigate the molecular composition of ex vivo slices of cancerous tumors, little is known about how variations in the cellular properties of cancer tissue can influence cancer biomarker ion images. To better understand the basis for variations in the abundances of cancer biomarker ions seen in MS images of relatively homogeneous ex vivo tumor samples, sections of snap frozen human breast cancer murine xenografts were subjected to desorption electrospray ionization mass spectrometry (DESI-MS) imaging...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28193008/two-new-devices-for-identifying-electrochemical-reaction-intermediates-with-desorption-electrospray-ionization-mass-spectrometry
#6
Heyong Cheng, Xin Yan, Richard N Zare
Desorption electrospray ionization mass spectrometry (DESI-MS) previously has been used to capture and identify transient intermediates in electrochemical redox reactions on a platinum-covered rotating waterwheel. We present here two different setups that use a flat surface with porous carbon tape as the working electrode, where analyte-containing microdroplets from the DESI probe contacted with electrolyte supplied onto the surface. One setup had the conducting carbon tape in the form of a grooved inclined plane; the other one was in the form of a flat plane that had the conducting carbon tape as its front surface...
January 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28192910/online-monitoring-of-enzymatic-reactions-using-time-resolved-desorption-electrospray-ionization-mass-spectrometry
#7
Si Cheng, Qiuhua Wu, He Xiao, Hao Chen
Electrospray ionization mass spectrometry (ESI-MS) is powerful for determining enzymatic reaction kinetics because of its soft ionization nature. However, it is limited to use ESI-favored solvents containing volatile buffers (e.g., ammonium acetate). In addition, lack of a quenching step for online ESI-MS reaction monitoring might introduce inaccuracy, due to the possible acceleration of reaction in the sprayed microdroplets. To overcome these issues, this study presents a new approach for online measuring enzymatic reaction kinetics using desorption electrospray ionization mass spectrometry (DESI-MS)...
February 9, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28190806/optimization-of-the-enzymatic-synthesis-of-biodiesel-from-terminalia-cattapa-l-kernel-oil-using-response-surface-methodology
#8
Dedy Suhendra, Erin Ryantin Gunawan, Arista Dewi Nurita, Desy Komalasari, Teguh Ardianto
The synthesis of biodiesel using Terminalia cattapa L. (local language: Ketapang) kernel oil and ethanol has been conducted. The effect of variable parameters of synthesis includes reaction time (3-7 h), temperature (35-60°C), amount of enzyme (0.1-0.3 g) and substrate molar ratio (ketapang kernel oil to ethanol, 1:1-1:3) were studied. Response surface methodology based on a five-level and four-variable central composite rotatable design were used to evaluate the interactive effects of the sinthesis parameters on the percentage yield of biodiedsel...
February 10, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28186642/higher-calcineurin-inhibitor-levels-predict-better-kidney-graft-survival-in-patients-with-de-novo-donor-specific-anti-hla-antibodies-a-cohort-study
#9
Marc-Antoine Béland, Isabelle Lapointe, Réal Noël, Isabelle Côté, Eric Wagner, Julie Riopel, Eva Latulippe, Olivier Désy, Stéphanie Béland, Ciara N Magee, Isabelle Houde, Sacha A De Serres
The development of de novo anti-HLA donor specific antibodies (dnDSA) is associated with poorer outcomes in kidney transplant recipients. Despite this, antibody screening post-transplant is not widespread, largely because the optimal management of patients with dnDSA remains undetermined. We hypothesized that in this population, calcineurin inhibitor blood levels would be an independent predictor of graft loss. We analyzed a cohort of unsensitized patients for whom anti-HLA antibody screening was performed prospectively post-transplant...
February 10, 2017: Transplant International: Official Journal of the European Society for Organ Transplantation
https://www.readbyqxmd.com/read/28160874/milestones-and-millennials-a-perfect-pairing-competency-based-medical-education-and-the-learning-preferences-of-generation-y
#10
REVIEW
Janeve R Desy, Darcy A Reed, Alexandra P Wolanskyj
Millennials are quickly becoming the most prevalent generation of medical learners. These individuals have a unique outlook on education and have different preferences and expectations than their predecessors. As evidenced by its implementation by the Accreditation Council for Graduate Medical Education in the United States and the Royal College of Physicians and Surgeons in Canada, competency based medical education is rapidly gaining international acceptance. Characteristics of competency based medical education can be perfectly paired with Millennial educational needs in several dimensions including educational expectations, the educational process, attention to emotional quotient and professionalism, assessment, feedback, and intended outcomes...
February 2017: Mayo Clinic Proceedings
https://www.readbyqxmd.com/read/28152521/lncsubpathway-a-novel-approach-for-identifying-dysfunctional-subpathways-associated-with-risk-lncrnas-by-integrating-lncrna-and-mrna-expression-profiles-and-pathway-topologies
#11
Yanjun Xu, Feng Li, Tan Wu, Yingqi Xu, Haixiu Yang, Qun Dong, Meiyu Zheng, Desi Shang, Chunlong Zhang, Yunpeng Zhang, Xia Li
Long non-coding RNAs (lncRNAs) play important roles in various biological processes, including the development of many diseases. Pathway analysis is a valuable aid for understanding the cellular functions of these transcripts. We have developed and characterized LncSubpathway, a novel method that integrates lncRNA and protein coding gene (PCG) expression with interactome data to identify disease risk subpathways that functionally associated with risk lncRNAs. LncSubpathway identifies the most relevance regions which are related with risk lncRNA set and implicated with study conditions through simultaneously considering the dysregulation extent of lncRNAs, PCGs and their correlations...
February 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28137351/congenital-mesoblastic-nephroma-presenting-with-refractory-hypertension-in-a-premature-neonate-a-case-study
#12
Tracey Robertson-Bell, Desi M Newberry, Amy J Jnah, Stephen D DeMeo
The most common nonencapsulated solid renal tumor in the neonatal period is congenital mesoblastic nephroma. Mesoblastic nephroma is a solid lesion originating within or extending from the renal parenchyma. These tumors proliferate rapidly, typically within 3-6 months after birth. Mesoblastic nephromas are stratified by classification as either classical (benign) or atypical (malignant); masses composed of both benign and malignant cells are also reported. The hallmark clinical manifestation of mesoblastic nephroma is a palpable abdominal mass, which may be accompanied by hypertension, hypercalcemia, hematuria, and polyuria...
January 1, 2017: Neonatal Network: NN
https://www.readbyqxmd.com/read/28112301/utility-of-neurological-smears-for-intrasurgical-brain-cancer-diagnostics-and-tumour-cell-percentage-by-desi-ms
#13
V Pirro, A K Jarmusch, C M Alfaro, E M Hattab, A A Cohen-Gadol, R Graham Cooks
Analysis of neurological smears by desorption electrospray ionization mass spectrometry (DESI-MS) is an emerging diagnostic strategy for intraoperative consultation in brain tumor resection. DESI-MS allows rapid sampling while providing accurate diagnostic information. We assess the chemical homogeneity of neurological smears using DESI-MS imaging and the quality of rapid DESI-MS diagnosis.
January 23, 2017: Analyst
https://www.readbyqxmd.com/read/28110652/ambient-mass-spectrometry-in-cancer-research
#14
Z Takats, N Strittmatter, J S McKenzie
Ambient ionization mass spectrometry was developed as a sample preparation-free alternative to traditional MS-based workflows. Desorption electrospray ionization (DESI)-MS methods were demonstrated to allow the direct analysis of a broad range of samples including unaltered biological tissue specimens. In contrast to this advantageous feature, nowadays DESI-MS is almost exclusively used for sample preparation intensive mass spectrometric imaging (MSI) in the area of cancer research. As an alternative to MALDI, DESI-MSI offers matrix deposition-free experiment with improved signal in the lower (<500m/z) range...
2017: Advances in Cancer Research
https://www.readbyqxmd.com/read/28109761/survivorship-of-metaphyseal-sleeves-in-revision-total-knee-arthroplasty
#15
Brian P Chalmers, Nicholas M Desy, Mark W Pagnano, Robert T Trousdale, Michael J Taunton
BACKGROUND: Metaphyseal fixation has promising early results in providing component stability and fixation in revision total knee arthroplasty (TKA). However, there are limited studies on midterm results of metaphyseal sleeves. We analyzed complications, rerevisions, and survivorship free of revision for aseptic loosening of metaphyseal sleeves in revision TKA. METHODS: Two hundred eighty patients with 393 metaphyseal sleeves (144 femoral, 249 tibial) implanted during revision TKA from 2006-2014 were reviewed...
December 14, 2016: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28105175/analysis-of-transcription-profile-to-reveal-altered-signaling-pathways-following-the-overexpression-of-human-desumoylating-isopeptidase-2-in-pancreatic-cancer-cells
#16
Yu-Yin Fu, Yu-Huan Kang, Cong-Cong Shen, Rui-Xue Wang, Lin Yu, Xin-Yue Li, Dan-Dan Cui, Jin-Liang Yang, Yu-Qin Yao, Lan-Tu Gou
Human desumoylating isopeptidase 2 (DESI-2) is a member of the DESI family and contains a conserved PPPDE1 domain. Previous studies have demonstrated that DESI-2 overexpression may induce cell apoptosis. In the present study, differentially expressed genes were analyzed using a transcription microarray in DESI-2 overexpressing PANC-1 pancreatic cancer cells. A total of 45,033 genes were examined by microarray, which identified 1,766 upregulated and 1,643 downregulated genes. A series of altered signaling pathways were analyzed, in which certain essential signaling factors, including retinoid X receptor (RXR), BH3 interacting-domain death agonist, Ras homolog gene family member A (RhoA) and Rho-associated protein kinase, were further investigated at the protein level...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28094338/mitochondrial-protein-import-functional-analysis-of-the-highly-diverged-tom22-orthologue-of-trypanosoma-brucei
#17
Jan Mani, Samuel Rout, Silvia Desy, André Schneider
The β-barrel protein Tom40 and the α-helically anchored membrane protein Tom22 are the only universally conserved subunits of the protein translocase of the mitochondrial outer membrane (TOM). Tom22 has an N-terminal cytosolic and a C-terminal intermembrane space domain. It occurs in two variants: one typified by the yeast protein which has a cytosolic domain containing a cluster of acidic residues, and a shorter variant typified by the plant protein that lacks this domain. Yeast-type Tom22 functions as a secondary protein import receptor and is also required for the stability of the TOM complex...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28035435/method-development-towards-qualitative-and-semi-quantitative-analysis-of-multiple-pesticides-from-food-surfaces-and-extracts-by-desorption-electrospray-ionization-mass-spectrometry-as-a-preselective-tool-for-food-control
#18
Stefanie Gerbig, Gerold Stern, Hubertus E Brunn, Rolf-Alexander Düring, Bernhard Spengler, Sabine Schulz
Direct analysis of fruit and vegetable surfaces is an important tool for in situ detection of food contaminants such as pesticides. We tested three different ways to prepare samples for the qualitative desorption electrospray ionization mass spectrometry (DESI-MS) analysis of 32 pesticides found on nine authentic fruits collected from food control. Best recovery rates for topically applied pesticides (88%) were found by analyzing the surface of a glass slide which had been rubbed against the surface of the food...
December 29, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28018400/identification-and-expression-analysis-of-candidate-genes-involved-in-carotenoid-biosynthesis-in-chickpea-seeds
#19
Mohammad K Rezaei, Amit Deokar, Bunyamin Tar'an
Plant carotenoids have a key role in preventing various diseases in human because of their antioxidant and provitamin A properties. Chickpea is a good source of carotenoid among legumes and its diverse germplasm and genome accessibility makes it a good model for carotenogenesis studies. The structure, location, and copy numbers of genes involved in carotenoid biosynthesis were retrieved from the chickpea genome. The majority of the single nucleotide polymorphism (SNPs) within these genes across five diverse chickpea cultivars was synonymous mutation...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28009574/p13-the-embl-macromolecular-crystallography-beamline-at-the-low-emittance-petra-iii-ring-for%C3%A2-high-and-low-energy-phasing-with-variable-beam-focusing
#20
Michele Cianci, Gleb Bourenkov, Guillaume Pompidor, Ivars Karpics, Johanna Kallio, Isabel Bento, Manfred Roessle, Florent Cipriani, Stefan Fiedler, Thomas R Schneider
The macromolecular crystallography P13 beamline is part of the European Molecular Biology Laboratory Integrated Facility for Structural Biology at PETRA III (DESY, Hamburg, Germany) and has been in user operation since mid-2013. P13 is tunable across the energy range from 4 to 17.5 keV to support crystallographic data acquisition exploiting a wide range of elemental absorption edges for experimental phase determination. An adaptive Kirkpatrick-Baez focusing system provides an X-ray beam with a high photon flux and tunable focus size to adapt to diverse experimental situations...
January 1, 2017: Journal of Synchrotron Radiation
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