keyword
https://read.qxmd.com/read/37176707/current-debates-in-the-management-of-visceral-artery-aneurysms-where-the-guidelines-collide
#21
EDITORIAL
Enrico Maria Marone, Luigi Federico Rinaldi
On one hand, the main difficulties in establishing a wide, evidence-based consensus about the best approach to visceral artery aneurysms (VAAs) and pseudoaneurysms (VAPAs) are the paucity of data, due to their rarity in the general population, and the extreme heterogeneity of this group of diseases, which encompasses different aneurysm types, with different degrees of rupture risks according to their anatomical locations [...].
May 4, 2023: Journal of Clinical Medicine
https://read.qxmd.com/read/37028687/isolation-of-aeromonas-salmonicida-subspecies-salmonicida-from-aquaculture-environment-in-india-polyphasic-identification-virulence-characterization-and-antibiotic-susceptibility
#22
JOURNAL ARTICLE
Subham Kumar Pradhan, Rajashree Devi, Md Idrish Raja Khan, Dibyendu Kamilya, Tanmoy Gon Choudhury, Janmejay Parhi
This study reports the polyphasic identification, characterization of virulence potential, and antibiotic susceptibility of Aeromonas salmonicida subspecies salmonicida COFCAU_AS, isolated from an aquaculture system in India. The physiological, biochemical, 16s rRNA gene sequencing and PAAS PCR test identified the strain as Aeromonas salmonicida. The MIY PCR tests established the subspecies as 'salmonicida'. The in vitro tests showed the isolated bacterium as haemolytic with casein, lipid, starch, and gelatin hydrolysis activity, indicating its pathogenic attributes...
June 2023: Microbial Pathogenesis
https://read.qxmd.com/read/36838503/the-pathogen-aeromonas-salmonicida-achromogenes-induces-fast-immune-and-microbiota-modifications-in-rainbow-trout
#23
JOURNAL ARTICLE
Baptiste Redivo, Nicolas Derôme, Patrick Kestemont, Valérie Cornet
Environmental stressors can disrupt the relationship between the microbiota and the host and lead to the loss of its functions. Among them, bacterial infection caused by Aeromonas salmonicida , the causative agent of furunculosis, results in high mortality in salmonid aquaculture. Here, rainbow trout were exposed to A. salmonicida achromogenes and its effects on the taxonomic composition and structure of the microbiota was assessed on different epithelia (gills, skin, and caudal fin) at 6 and 72 h post-infection (hpi) using the V1-V3 region of the 16S rRNA sequencing...
February 20, 2023: Microorganisms
https://read.qxmd.com/read/36786596/differential-effects-of-rhodococcus-equi-virulence-associated-proteins-on-macrophages-and-artificial-lipid-membranes
#24
JOURNAL ARTICLE
Philipp Hansen, Thomas Haubenthal, Caroline Reiter, Jana Kniewel, Karla Bosse-Plois, Hartmut H Niemann, Kristine von Bargen, Albert Haas
<u>V</u>irulence-<u>a</u>ssociated <u>p</u>rotein <u>A</u> (VapA) of Rhodococcus equi is a pathogenicity factor required for the multiplication of virulent R. equi strains within spacious macrophage vacuoles. The production of VapA is characteristic for R. equi isolates from pneumonic foals. VapB and VapN proteins in R. equi isolates from infected pig (VapB) and cattle (VapN) have amino acid sequences very similar to VapA and consequently have been assumed to be its functional correlates...
February 14, 2023: Microbiology Spectrum
https://read.qxmd.com/read/36627747/specific-preadaptations-of-rhodococcus-equi-cooperate-with-its-virulence-associated-protein-a-during-macrophage-infection
#25
JOURNAL ARTICLE
Thomas Haubenthal, Philipp Hansen, Ina Krämer, Mélanie Gindt, Alexandra Jünger-Leif, Olaf Utermöhlen, Albert Haas
Gram-positive Rhodococcus equi (Prescotella equi) is a lung pathogen of foals and immunocompromised humans. Intra-macrophage multiplication requires production of the bacterial Virulence-associated protein A (VapA) which is released into the phagosome lumen. VapA pH-neutralizes intracellular compartments allowing R. equi to multiply in an atypical macrophage phagolysosome. Here, we show that VapA does not support intra-macrophage growth of several other bacterial species demonstrating that only few bacteria have the specific preadaptations needed to profit from VapA...
January 10, 2023: Molecular Microbiology
https://read.qxmd.com/read/36601098/spontaneous-rupture-of-splenic-artery-pseudoaneurysm
#26
Andriy Hordiychuk, Daniel Mehanna
False aneurysm or visceral artery pseudoaneurysm (VAPA) results from a tear in the vessel wall due to trauma with subsequent periarterial haematoma formation. VAPA is relatively rare, with a reported incidence of 0.1-0.2%, although the actual incidence is not known since many are asymptomatic. Splenic artery pseudoaneurysm is even more rare pathology. Only around 200 cases have been described in the literature. The case report below describes a spontaneous rupture of splenic artery pseudoaneurysm.
December 2022: Journal of Surgical Case Reports
https://read.qxmd.com/read/36513538/description-of-physical-activity-in-children-with-juvenile-idiopathic-arthritis-using-accelerometry
#27
JOURNAL ARTICLE
L Jonyk, Y Sagawa Junior, N Tordi, C Ballot Schmit
OBJECTIVES: Juvenile idiopathic arthritis (JIA) can lead to functional impotence and reduced physical activity (PA) in patients. The objective of this study was to monitor the daily PA of children aged 6-18 years with JIA using accelerometry based on disease activity and individual abilities, and to compare this with age recommendations. METHODS: Patients volunteering for the study wore an accelerometer for 7 consecutive days; data were evaluated using a wGT3X ActiGraph™...
December 10, 2022: Archives de Pédiatrie: Organe Officiel de la Sociéte Française de Pédiatrie
https://read.qxmd.com/read/36423204/evaluation-of-serum-concentration-of-acute-phase-proteins-haptoglobin-and-serum-amyloid-a-in-the-affected-arabian-foals-with-rhodococcosis
#28
JOURNAL ARTICLE
Ali Hassanpour, Sina Moghaddam
BACKGROUND: Early detection of Rhodococcus equi pneumonia in foals is essential for horse health and for veterinarians. OBJECTIVES: This study aimed to demonstrate the usefulness of assessing the serum concentration of acute-phase proteins (APPs) in the early diagnosis of pneumonia. METHODS: The study evaluated APPs in 19 Arabian foals with R. equi pneumonia and compared them with 18 normal Arabian foals in equestrian clubs in Tabriz, Iran...
November 24, 2022: Veterinary Medicine and Science
https://read.qxmd.com/read/36408828/cholesterol-transfer-via-endoplasmic-reticulum-contacts-mediates-lysosome-damage-repair
#29
JOURNAL ARTICLE
Maja Radulovic, Eva Maria Wenzel, Sania Gilani, Lya Kk Holland, Alf Håkon Lystad, Santosh Phuyal, Vesa M Olkkonen, Andreas Brech, Marja Jäättelä, Kenji Maeda, Camilla Raiborg, Harald Stenmark
Lysosome integrity is essential for cell viability, and lesions in lysosome membranes are repaired by the ESCRT machinery. Here, we describe an additional mechanism for lysosome repair that is activated independently of ESCRT recruitment. Lipidomic analyses showed increases in lysosomal phosphatidylserine and cholesterol after damage. Electron microscopy demonstrated that lysosomal membrane damage is rapidly followed by the formation of contacts with the endoplasmic reticulum (ER), which depends on the ER proteins VAPA/B...
December 15, 2022: EMBO Journal
https://read.qxmd.com/read/36332440/older-adults-experiences-using-alexa
#30
JOURNAL ARTICLE
Sarit Orlofsky, Kathryn Wozniak
The number of adults in the United States age 65 and older is expected to increase to 81 million in 2040 and 95 million in 2060. Voice-activated personal assistants (VAPAs), such as Amazon's Alexa, have the potential to ensure that older adults can successfully age in place using smart home technology to support health, wellness, social engagement, and daily functioning at home. To further explore this potential, twelve home-dwelling older adults aged 65 and older who had used the Amazon Alexa VAPA device for over six months were interviewed to learn about their perceptions and experiences with the use of the device and to gain insights into how this technology has shaped their aging experience...
October 28, 2022: Geriatric Nursing
https://read.qxmd.com/read/36198318/calcium-transients-on-the-er-surface-trigger-liquid-liquid-phase-separation-of-fip200-to-specify-autophagosome-initiation-sites
#31
JOURNAL ARTICLE
Qiaoxia Zheng, Yong Chen, Di Chen, Hongyu Zhao, Yun Feng, Quan Meng, Yan Zhao, Hong Zhang
The mechanism that initiates autophagosome formation on the ER in multicellular organisms is elusive. Here, we showed that autophagy stimuli trigger Ca2+ transients on the outer surface of the ER membrane, whose amplitude, frequency, and duration are controlled by the metazoan-specific ER transmembrane autophagy protein EPG-4/EI24. Persistent Ca2+ transients/oscillations on the cytosolic ER surface in EI24-depleted cells cause accumulation of FIP200 autophagosome initiation complexes on the ER. This defect is suppressed by attenuating ER Ca2+ transients...
September 30, 2022: Cell
https://read.qxmd.com/read/36192520/development-of-two-recombinase-polymerase-amplification-exo-rpa-exo-and-lateral-flow-dipstick-rpa-lfd-techniques-for-the-rapid-visual-detection-of-aeromonas-salmonicida
#32
JOURNAL ARTICLE
Shun Zhou, Xujia Zheng, Zongrui Yang, Qing Huang, Jingyuan Yi, Lin Su, Baoshan Guo, Yunji Xiu
Aeromonas salmonicida is the pathogen underlying furunculosis, causing a septicemic infection that influences both salmonid and non-salmonid fish. Early diagnosis of these contagions is essential for disease surveillance and prevention, so a rapid and sensitive approach is needed. Herein, a recombinase polymerase amplification EXO (RPA-EXO) assay and RPA with a lateral flow dipstick (RPA-LFD) were produced for testing A. salmonicida. The RPA-EXO and RPA-LFD primer sets were devised based on the conserved fragment sequence of the vapA gene...
December 2022: Marine Biotechnology
https://read.qxmd.com/read/36174556/er-golgi-localized-proteins-tmed2-and-tmed10-control-the-formation-of-plasma-membrane-lipid-nanodomains
#33
JOURNAL ARTICLE
Muhammad U Anwar, Oksana A Sergeeva, Laurence Abrami, Francisco S Mesquita, Ilya Lukonin, Triana Amen, Audrey Chuat, Laura Capolupo, Prisca Liberali, Giovanni D'Angelo, F Gisou van der Goot
To promote infections, pathogens exploit host cell machineries such as structural elements of the plasma membrane. Studying these interactions and identifying molecular players are ideal for gaining insights into the fundamental biology of the host cell. Here, we used the anthrax toxin to screen a library of 1,500 regulatory, cell-surface, and membrane trafficking genes for their involvement in the intoxication process. We found that endoplasmic reticulum (ER)-Golgi-localized proteins TMED2 and TMED10 are required for toxin oligomerization at the plasma membrane of human cells, an essential step dependent on localization to cholesterol-rich lipid nanodomains...
September 22, 2022: Developmental Cell
https://read.qxmd.com/read/36169100/large-oncosome-loaded-vapa-promotes-bone-tropic-metastasis-of-hepatocellular-carcinoma-via-formation-of-osteoclastic-pre-metastatic-niche
#34
JOURNAL ARTICLE
Shuxia Zhang, Xinyi Liao, Suwen Chen, Wanying Qian, Man Li, Yingru Xu, Meisongzhu Yang, Xincheng Li, Shuang Mo, Miaoling Tang, Xingui Wu, Yameng Hu, Ziwen Li, Ruyuan Yu, Ainiwaerjiang Abudourousuli, Libing Song, Jun Li
Tumor-derived extracellular vesicles (EVs) function as critical mediators in selective modulation of the microenvironment of distant organs to generate a pre-metastatic niche that facilitates organotropic metastasis. Identifying the organ-specific molecular determinants of EVs can develop potential anti-metastatic therapeutic targets. In the current study, large oncosomes (LOs), atypically large cancer-derived EVs, are found to play a crucial role in facilitating bone-tropic metastasis of hepatocellular carcinoma (HCC) cells by engineering an osteoclastic pre-metastatic niche and establishing a vicious cycle between the osteoclasts and HCC cells...
November 2022: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://read.qxmd.com/read/36072397/exploring-the-feasibility-of-virtually-delivered-auricular-point-acupressure-in-self-managing-chronic-pain-qualitative-study
#35
JOURNAL ARTICLE
Jennifer Kawi, Chao Hsing Yeh, Nada Lukkahatai, Robin L Hardwicke, Thomas Murphy, Paul J Christo
Background: Chronic pain remains highly prevalent. Current pharmacological and non-pharmacological strategies have not adequately managed chronic pain which has contributed to disability and high healthcare costs. With existing challenges in providing adequate pain care and access, we tested vAPA, a virtually delivered, self-management intervention using Auricular Point Acupressure (APA) by mobile app and virtual consultations (telehealth). Our key purpose was to evaluate the feasibility of the vAPA in self-managing chronic pain in preparation for a future randomized controlled trial...
2022: Evidence-based Complementary and Alternative Medicine: ECAM
https://read.qxmd.com/read/35993764/laboratory-plasticware-induces-expression-of-a-bacterial-virulence-factor
#36
JOURNAL ARTICLE
Philipp Hansen, Kristine von Bargen, Alexandra Jünger-Leif, Albert Haas
Pollution with microplastic has become a prime environmental concern. The various ways in which human-made polymers and microorganisms interact are little understood, and this is particularly true for microplastic and pathogenic microorganisms. Previous reports demonstrated that expression of central virulence-associated protein A (VapA) of the pathogenic bacterium Rhodococcus equi is shut off at 30°C, whereas it is strongly expressed at 37°C, a temperature which may serve as an intrahost cue. Here, we show that cultivation at 30°C in disposable plastic tubes increases mRNA levels of vapA 70-fold compared to growth in conventional glass tubes...
August 22, 2022: MSphere
https://read.qxmd.com/read/35981539/dual-control-of-formin-nucleated-actin-assembly-by-the-chromatin-and-er-in-mouse-oocytes
#37
JOURNAL ARTICLE
HaiYang Wang, Jinrong Hu, Kexi Yi, Zhiming Ma, XinJie Song, Yaelim Lee, Petr Kalab, Alexander D Bershadsky, Yansong Miao, Rong Li
The first asymmetric meiotic cell divisions in mouse oocytes are driven by formin 2 (FMN2)-nucleated actin polymerization around the spindle. In this study, we investigated how FMN2 is recruited to the spindle peripheral ER and how its activity is regulated in mouse meiosis I (MI) oocytes. We show that this process is regulated by the Ran GTPase, a conserved mediator of chromatin signal, and the ER-associated protein VAPA. FMN2 contains a nuclear localization sequence (NLS) within a domain (SLD) previously shown to be required for FMN2 localization to the spindle periphery...
August 12, 2022: Current Biology: CB
https://read.qxmd.com/read/35756994/vap-proteins-from-organelle-tethers-to-pathogenic-host-interactors-and-their-role-in-neuronal-disease
#38
REVIEW
Suzan Kors, Joseph L Costello, Michael Schrader
Vesicle-associated membrane protein (VAMP)-associated proteins (VAPs) are ubiquitous ER-resident tail-anchored membrane proteins in eukaryotic cells. Their N-terminal major sperm protein (MSP) domain faces the cytosol and allows them to interact with a wide variety of cellular proteins. Therefore, VAP proteins are vital to many cellular processes, including organelle membrane tethering, lipid transfer, autophagy, ion homeostasis and viral defence. Here, we provide a timely overview of the increasing number of VAPA/B binding partners and discuss the role of VAPA/B in maintaining organelle-ER interactions and cooperation...
2022: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/35707941/virulence-plasmids-in-clinical-isolates-of-rhodococcus-equi-from-sick-foals-in-the-netherlands
#39
JOURNAL ARTICLE
S Takai, M Ohashi, Y Suzuki, Y Sasaki, T Kakuda, E M Broens, J A Wagenaar, E van Duijkeren
Clinical samples from 123 foals with suspected rhodococcosis submitted to the Veterinary Microbiological Diagnostic Centre of the Faculty of Veterinary Medicine between 1993 and 2006 were tested for the presence of the virulence gene vapA. Of the 123 samples, 120 were vapA-positive and 3 vapA-negative Rhodococcus equi were isolated. The 120 vapA-positive R. equi were isolated from 70 tracheal wash, 19 lung tissues, 7 lymph nodes, 6 synovial fluids, 13 abscesses or pus and single isolates from the uterus, gut, cerebrospinal fluid, abdomen fluid and faeces...
October 2022: Letters in Applied Microbiology
https://read.qxmd.com/read/35683341/imaging-guided-percutaneous-puncture-and-embolization-of-visceral-pseudoaneurysms-feasibility-and-outcomes
#40
JOURNAL ARTICLE
Serena Carriero, Carolina Lanza, Pierpaolo Biondetti, Matteo Renzulli, Cristian Bonelli, Filippo Piacentino, Federico Fontana, Massimo Venturini, Gianpaolo Carrafiello, Anna Maria Ierardi
Visceral artery pseudoaneurysms (VAPAs) are the most frequently diagnosed pseudoaneurysms (PSAs). PSAs can be asymptomatic or symptomatic. The aim of our study was to evaluate the safety and effectiveness of percutaneous embolization of VAPAs performed on patients with an unfeasible trans-arterial approach. Fifteen patients with fifteen visceral PSAs, with a median dimension of 21 mm (IQR 20-24 mm), were retrospectively analyzed. No patients were suitable for trans-arterial catheterization and therefore a percutaneous approach was chosen...
May 24, 2022: Journal of Clinical Medicine
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