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https://www.readbyqxmd.com/read/28868569/genetic-analysis-of-12-x-str-loci-in-the-serbian-population-from-vojvodina-province
#1
Igor Veselinović, Dušan Vapa, Mihajla Djan, Nevena Veličković, Tanja Veljović, Galina Petrić
The analysis of 12 X-STR loci included in the Investigator® Argus X-12 kit was performed on a sample of 325 unrelated persons from Vojvodina Province, Republic of Serbia. No significant differences were observed in the allele frequencies in males and females. Heterozygosity values among the studied loci ranged from 67.62 to 94.28%. All loci in female individuals were consistent with the Hardy-Weinberg equilibrium test. The combined power of discrimination values in male and female individuals was 0.9999999994 and 0...
September 3, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28698274/noroviruses-co-opt-the-function-of-host-proteins-vapa-and-vapb-for-replication-via-a-phenylalanine-phenylalanine-acidic-tract-motif-mimic-in-nonstructural-viral-protein-ns1-2
#2
Broc T McCune, Wei Tang, Jia Lu, James B Eaglesham, Lucy Thorne, Anne E Mayer, Emily Condiff, Timothy J Nice, Ian Goodfellow, Andrzej M Krezel, Herbert W Virgin
The Norovirus genus contains important human pathogens, but the role of host pathways in norovirus replication is largely unknown. Murine noroviruses provide the opportunity to study norovirus replication in cell culture and in small animals. The human norovirus nonstructural protein NS1/2 interacts with the host protein VAMP-associated protein A (VAPA), but the significance of the NS1/2-VAPA interaction is unexplored. Here we report decreased murine norovirus replication in VAPA- and VAPB-deficient cells. We characterized the role of VAPA in detail...
July 11, 2017: MBio
https://www.readbyqxmd.com/read/28690290/rescue-of-an-intracellular-avirulent-rhodococcus-equi-replication-defect-by-the-extracellular-addition-of-virulence-associated-protein-a
#3
Nuttapone Sangkanjanavanich, Masanori Kawai, Tsutomu Kakuda, Shinji Takai
Rhodococcus equi is a facultative intracellular bacterium that can escape from bactericidal mechanisms associated with phagocytosis. Virulence-associated protein A (VapA), encoded on a virulence-associated plasmid, is essential for intracellular survival in macrophages, but its function is not known. Here, we show that the extracellular addition of recombinant glutathione S-transferase (GST)-VapA fusion protein rescued the intracellular replication defect of a mutant lacking the vapA gene. Furthermore, the virulence-plasmid-cured strain could also multiply to nearly wild-type levels by the addition of GST-VapA...
August 4, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28659059/endovascular-treatment-of-visceral-artery-aneurysms-and-pseudoaneurysms-in-100-patients-covered-stenting-vs-transcatheter-embolization
#4
Massimo Venturini, Paolo Marra, Michele Colombo, Marco Alparone, Giulia Agostini, Luca Bertoglio, Claudio Sallemi, Marco Salvioni, Simone Gusmini, Gianpaolo Balzano, Renata Castellano, Luca Aldrighetti, Yamume Tshomba, Massimo Falconi, Germano Melissano, Francesco De Cobelli, Roberto Chiesa, Alessandro Del Maschio
PURPOSE: To retrospectively report a large single-center experience of visceral artery aneurysms (VAAs) and pseudoaneurysms (VAPAs) treated with covered stenting (CS) as the first therapeutic option vs transcatheter embolization (TE). METHODS: One hundred patients (mean age 59±14 years; 58 men) underwent 59 elective and 41 emergent endovascular procedures to treat 51 VAAs and 49 VAPAs. Seventy patients had TE and 30 received CS (27 Viabahn and 3 coronary stent grafts)...
June 1, 2017: Journal of Endovascular Therapy
https://www.readbyqxmd.com/read/28642343/differential-distribution-of-vapa-positive-rhodococcus-equi-in-affected-and-unaffected-horse-breeding-farms
#5
S Petry, C Sévin, M A Fleury, F Duquesne, N Foucher, C Laugier, M Henry-Amar, J Tapprest
No abstract text is available yet for this article.
June 22, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28553279/vaccination-of-mice-with-virulence-associated-protein-g-vapg-antigen-confers-partial-protection-against-rhodococcus-equi-infection-through-induced-humoral-immunity
#6
Marcel M Trevisani, Ebert S Hanna, Aline F Oliveira, Silvia A Cardoso, Maria C Roque-Barreira, Sandro G Soares
Rhodococcus equi is a facultative intracellular bacterium causing severe pyogranulomatous pneumonia, ulcerative enterocolitis, and mesenteric lymphadenopathy in foals aged less than 6 months. Less frequently, this pathogen affects various other species, such as pigs, cattle, cats, and even humans. Although rhodococcosis is treated with a combination of antimicrobial agents, resistance is developed in some cases, and thus, antimicrobial susceptibility must be monitored and managed. Considering these limitations of the current therapy and unavailability of a vaccine to prevent the disease, research is particularly focused on the development of an effective vaccine against rhodococcosis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28279794/involvement-of-luxs-in-aeromonas-salmonicida-metabolism-virulence-and-infection-in-atlantic-salmon-salmo-salar-l
#7
Lingjie Meng, Yishuai Du, Pengfei Liu, Xian Li, Ying Liu
Quorum sensing is a bacterial density dependent communication system, which regarded to regulate co-operative behaviors of community and mediated by extracellular signal molecules named autoinducers (AI). Among various signals, autoinducer-2 (AI-2) is believed to be the messengers inter species and produced by LuxS. For Aeromonas salmonicida (A. salmonicida), an opportunistic pathogen to many cold-water teleost, little information has been known about the function of AI-2 and LuxS. Therefore, our aim was to preliminarily clarify the function of LuxS in A...
May 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28149575/veno-venous-extracorporeal-membrane-oxygenation-cannulation-techniques
#8
REVIEW
Carlo Banfi, Matteo Pozzi, Nils Siegenthaler, Marie-Eve Brunner, Didier Tassaux, Jean-Francois Obadia, Karim Bendjelid, Raphaël Giraud
The development of extracorporeal membrane oxygenation (ECMO) technology allows a new approach for the intensive care management of acute cardiac and/or respiratory failure in adult patients who are not responsive to conventional treatment. Current ECMO therapies provide a variety of options for the multidisciplinary teams who are involved in the management of these critically ill patients. In this regard, veno-venous ECMO (VV-ECMO) can provide quite complete respiratory support, even if this highly complex technique presents substantial risks, such as bleeding, thromboembolic events and infection...
December 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28124475/genome-wide-two-locus-interaction-analysis-identifies-multiple-epistatic-snp-pairs-that-confer-risk-of-prostate-cancer-a-cross-population-study
#9
Jiawei Shen, Zhiqiang Li, Zhijian Song, Jianhua Chen, Yongyong Shi
Prostate cancer is one of the most common carcinomas among adult males. Recently, genome-wide association studies (GWAS) have identified several susceptibility genes of prostate cancer. However, these single locus results can only explain a small proportion of the genetic etiology. In order to understand how multiple genetic variants may contribute to the penetrance of prostate cancer, we conducted a genome-wide SNP-SNP interaction study in four populations, involving 5,269 cases and 5,289 controls. We exhaustively evaluated all pairs of SNP-SNP interactions for 661,658 SNPs that were consensus in all four groups, and then performed a meta-analysis to combine the results...
May 1, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28122544/molecular-characterization-of-rhodococcus-equi-isolates-from-horses-in-poland-pvapa-characteristics-and-plasmid-new-variant-85-kb-type-v
#10
Lucjan Witkowski, Magdalena Rzewuska, Shinji Takai, Dorota Chrobak-Chmiel, Magdalena Kizerwetter-Świda, Małgorzata Feret, Marta Gawryś, Maciej Witkowski, Jerzy Kita
BACKGROUND: Rhodococcus equi is one of the most significant bacterial pathogens affecting foals up to 6 months of age worldwide. Rhodococcosis is present in Poland however information about molecular characterization of R. equi isolates is scarce. This study describes molecular characterization of Rhodococcus equi infection on 13 horse breeding farms in Poland between 2001 and 2012. Samples were collected by tracheobronchial aspiration from pneumonic foals or during necropsy. The R. equi isolates were genotyped by plasmid profiling and pulsed-field gel electrophoresis...
January 26, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28108527/incredibly-close-a-newly-identified-peroxisome-er-contact-site-in-humans
#11
Maya Schuldiner, Einat Zalckvar
Peroxisomes are tiny organelles that control important and diverse metabolic processes via their interplay with other organelles, including the endoplasmic reticulum (ER). In this issue, Costello et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201607055) and Hua et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201608128) identify a peroxisome-ER contact site in human cells held together by a tethering complex of VAPA/B (vesicle-associated membrane protein-associated proteins A/B) and ACBD5 (acyl Co-A binding protein 5)...
February 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28108526/vaps-and-acbd5-tether-peroxisomes-to-the-er-for-peroxisome-maintenance-and-lipid-homeostasis
#12
Rong Hua, Derrick Cheng, Étienne Coyaud, Spencer Freeman, Erminia Di Pietro, Yuqing Wang, Adriano Vissa, Christopher M Yip, Gregory D Fairn, Nancy Braverman, John H Brumell, William S Trimble, Brian Raught, Peter K Kim
Lipid exchange between the endoplasmic reticulum (ER) and peroxisomes is necessary for the synthesis and catabolism of lipids, the trafficking of cholesterol, and peroxisome biogenesis in mammalian cells. However, how lipids are exchanged between these two organelles is not understood. In this study, we report that the ER-resident VAMP-associated proteins A and B (VAPA and VAPB) interact with the peroxisomal membrane protein acyl-CoA binding domain containing 5 (ACBD5) and that this interaction is required to tether the two organelles together, thereby facilitating the lipid exchange between them...
February 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/27865011/vital-signs-as-predictors-for-aggression-in-hospital-patients-vapa
#13
Julie Considine, Debra Berry, Rebecca Johnson, Natisha Sands
AIMS AND OBJECTIVES: To examine and describe the relationship between physiological status and violent and aggressive behaviours in hospital patients. BACKGROUND: The majority of adverse events are preceded by physiological abnormalities; whether physiological deterioration is a predictor of violent or aggressive behaviours remains unknown. DESIGN: Prospective case-control study. METHODS: Prospective audit of 999 patients from two major health services in Melbourne, Australia...
November 16, 2016: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/27762083/the-rhodococcus-equi-virulence-protein-vapa-disrupts-endolysosome-function-and-stimulates-lysosome-biogenesis
#14
Adam P Rofe, Luther J Davis, Jean L Whittingham, Elizabeth C Latimer-Bowman, Anthony J Wilkinson, Paul R Pryor
Rhodococcus equi (R. equi) is an important pulmonary pathogen in foals that often leads to the death of the horse. The bacterium harbors a virulence plasmid that encodes numerous virulence-associated proteins (Vaps) including VapA that is essential for intracellular survival inside macrophages. However, little is known about the precise function of VapA. Here, we demonstrate that VapA causes perturbation to late endocytic organelles with swollen endolysosome organelles having reduced Cathepsin B activity and an accumulation of LBPA, LC3 and Rab7...
April 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/27747295/influence-of-plasmid-type-on-the-replication-of-rhodococcus-equi-in-host-macrophages
#15
Jennifer M Willingham-Lane, Londa J Berghaus, Steeve Giguère, Mary K Hondalus
The soil-dwelling, saprophytic actinomycete Rhodococcus equi is a multihost, facultative intracellular pathogen of macrophages. When inhaled by susceptible foals, it causes severe bronchopneumonia. It is also a pathogen of pigs, which may develop submaxillary lymphadenitis upon exposure. R. equi isolates obtained from foals and pigs possess conjugative plasmids housing a pathogenicity island (PAI) containing a novel family of genes of unknown function called the virulence-associated protein or vap family. The PAI regions of the equine and swine plasmids differ in vap gene composition, with equine isolates possessing six vap genes, including the major virulence determinant vapA, while the PAIs of swine isolates house vapB and five other unique vap genes...
September 2016: MSphere
https://www.readbyqxmd.com/read/27493140/development-of-a-loop-mediated-isothermal-amplification-method-for-detecting-virulent-rhodococcus-equi
#16
Yuta Kinoshita, Hidekazu Niwa, Tohru Higuchi, Yoshinari Katayama
Rhodococcus equi is the most important causative bacterium of severe pneumonia in foals. We report herein the development of a specific loop-mediated isothermal amplification (LAMP) assay, which targets a gene encoding vapA for detecting virulent R. equi The detection limit of the LAMP assay was 10(4) colony forming units (CFU)/mL, which was equal to 10 CFU/reaction. The clinical efficacy of the LAMP assay was compared with those of 2 published PCR-based methods: nested PCR and quantitative real-time (q)PCR...
September 2016: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/27493011/aeromonas-salmonicida-subsp-salmonicida-strains-isolated-from-chinese-freshwater-fish-contain-a-novel-genomic-island-and-possible-regional-specific-mobile-genetic-elements-profiles
#17
Meng Long, Tue K Nielsen, Jørgen J Leisner, Lars H Hansen, Zhi X Shen, Qian Q Zhang, Aihua Li
Two strains of Aeromonas salmonicida, YK and BG, were isolated from largemouth bronze gudgeon and northern whitefish in China, and identified as A. salmonicida subsp. salmonicida based on phylogenetic analysis of vapA and 16S rRNA gene sequences. YK and BG originated from freshwater fish, one of which belonged to the cyprinid family, and the strains showed a difference in virulence. Subsequently, we performed whole genome sequencing of the strains, and comparison of their genomic sequences to the genome of the A449 reference strain revealed various genomic rearrangements, including a new variant of the genomic island AsaGEI in BG, designated as AsaGEI2c This is the first report on a GEI of A...
September 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27419871/endosome-er-contacts-control-actin-nucleation-and-retromer-function-through-vap-dependent-regulation-of-pi4p
#18
Rui Dong, Yasunori Saheki, Sharan Swarup, Louise Lucast, J Wade Harper, Pietro De Camilli
VAP (VAPA and VAPB) is an evolutionarily conserved endoplasmic reticulum (ER)-anchored protein that helps generate tethers between the ER and other membranes through which lipids are exchanged across adjacent bilayers. Here, we report that by regulating PI4P levels on endosomes, VAP affects WASH-dependent actin nucleation on these organelles and the function of the retromer, a protein coat responsible for endosome-to-Golgi traffic. VAP is recruited to retromer budding sites on endosomes via an interaction with the retromer SNX2 subunit...
July 14, 2016: Cell
https://www.readbyqxmd.com/read/27312368/rhodococcus-equi-infections-in-dogs
#19
L K Bryan, S D Clark, J Díaz-Delgado, S D Lawhon, J F Edwards
Five cases of Rhodococcus equi infection in dogs were identified from 2003 to 2014. Three of the dogs had severe, internal lesions attributable to R. equi that have not been previously described: endophthalmitis, endocarditis, and suppurative pleuropneumonia. Isolates from 4 of the dogs were analyzed by polymerase chain reaction for Rhodococcus virulence-associated plasmid (vap) genes. One isolate was vapA-positive, 2 lacked a virulence plasmid, and 1 carried the novel vapN-associated plasmid (pVAPN) recently characterized in bovine isolates...
January 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/27234339/molecular-epidemiology-of-rhodococcus-equi-in-slaughtered-swine-cattle-and-horses-in-poland
#20
Lucjan Witkowski, Magdalena Rzewuska, Shinji Takai, Magdalena Kizerwetter-Świda, Jerzy Kita
BACKGROUND: Rhodococcus equi is an emerging zoonotic presumably foodborne pathogen. Since the data on the worldwide prevalence of R. equi in meat animals are scarce, the present study aimed to investigate the molecular epidemiology of R. equi in swine, cattle and horse carcasses intended for human consumption in Poland. RESULTS: Totally 1028 lymph node samples were examined. R. equi was isolated from 26.6 % (105/395) swine and 1.3 % (3/234) bovine healthy submaxillary lymph nodes...
May 27, 2016: BMC Microbiology
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