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https://www.readbyqxmd.com/read/28081443/the-bacterial-t6ss-effector-evpp-prevents-nlrp3-inflammasome-activation-by-inhibiting-the-ca-2-dependent-mapk-jnk-pathway
#1
Hao Chen, Dahai Yang, Fajun Han, Jinchao Tan, Lingzhi Zhang, Jingfan Xiao, Yuanxing Zhang, Qin Liu
Inflammasome activation is an important innate immune defense mechanism against bacterial infection, and in return, bacteria express virulence determinants that counteract inflammasome activation. Many such effectors are secreted into host cells via specialized bacterial secretion systems. Here, the intracellular pathogenic bacterium Edwardsiella tarda was demonstrated to activate NLRC4 and NLRP3 inflammasomes via a type III secretion system (T3SS), and to inhibit NLRP3 inflammasome via a type VI secretion system (T6SS), indicating the antagonistic roles of these systems in inflammasome signaling...
January 11, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28060574/the-hcp-proteins-fused-with-diverse-extended-toxin-domains-represent-a-novel-pattern-of-antibacterial-effectors-in-type-vi-secretion-systems
#2
Jiale Ma, Zihao Pan, Jinhu Huang, Min Sun, Chengping Lu, Huochun Yao
The type VI secretion system (T6SS) is a widespread molecular weapon deployed by many bacterial species to target eukaryotic host cells or rival bacteria. Using a dynamic injection mechanism, diverse effectors can be delivered by T6SS directly into recipient cells. Here, we report a new family of T6SS effectors encoded by extended Hcps carrying diverse toxin domains. Bioinformatic analyses revealed that these Hcps with C-terminal extension toxins, designated as Hcp-ET, exist widely in the Enterobacteriaceae...
January 6, 2017: Virulence
https://www.readbyqxmd.com/read/28045455/the-pseudomonas-putida-t6ss-is-a-plant-warden-against-phytopathogens
#3
Patricia Bernal, Luke P Allsopp, Alain Filloux, María A Llamas
Bacterial type VI secretion systems (T6SSs) are molecular weapons designed to deliver toxic effectors into prey cells. These nanomachines have an important role in inter-bacterial competition and provide advantages to T6SS active strains in polymicrobial environments. Here we analyze the genome of the biocontrol agent Pseudomonas putida KT2440 and identify three T6SS gene clusters (K1-, K2- and K3-T6SS). Besides, 10 T6SS effector-immunity pairs were found, including putative nucleases and pore-forming colicins...
January 3, 2017: ISME Journal
https://www.readbyqxmd.com/read/28027803/rules-of-engagement-the-type-vi-secretion-system-in-vibrio-cholerae
#4
REVIEW
Avatar Joshi, Benjamin Kostiuk, Andrew Rogers, Jennifer Teschler, Stefan Pukatzki, Fitnat H Yildiz
Microbial species often exist in complex communities where they must avoid predation and compete for favorable niches. The type VI secretion system (T6SS) is a contact-dependent bacterial weapon that allows for direct killing of competitors through the translocation of proteinaceous toxins. Vibrio cholerae is a Gram-negative pathogen that can use its T6SS during antagonistic interactions with neighboring prokaryotic and eukaryotic competitors. The T6SS not only promotes V. cholerae's survival during its aquatic and host life cycles, but also influences its evolution by facilitating horizontal gene transfer...
December 24, 2016: Trends in Microbiology
https://www.readbyqxmd.com/read/27974393/temperature-regulated-expression-of-type-vi-secretion-systems-in-fish-pathogen-pseudomonas-plecoglossicida-revealed-by-comparative-secretome-analysis
#5
Zhen Tao, Tao Zhou, Suming Zhou, Guoliang Wang
Pseudomonas plecoglossicida is a facultative fish pathogen. Recent studies showed that P. plecoglossicida infection in fish was associated with temperature. The aim of this study was to compare the secretomes of P. plecoglossicida cultured in vitro at representative temperatures for pathogenic (20°C) and less pathogenic (30°C) phenotypes. Thirteen proteins in the culture supernatants of P. plecoglossicida showed significant difference in abundance at 20 vs. 30°C. Four proteins were strongly increased at 20°C, including two hemolysin co-regulated proteins (Hcp) that are part of the bacterial type VI secretion system (T6SS), flagellin and an unknown protein...
November 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27920034/domestication-of-a-housekeeping-transglycosylase-for-assembly-of-a-type-vi-secretion-system
#6
Yoann G Santin, Eric Cascales
The type VI secretion system (T6SS) is an anti-bacterial weapon comprising a contractile tail anchored to the cell envelope by a membrane complex. The TssJ, TssL, and TssM proteins assemble a 1.7-MDa channel complex that spans the cell envelope, including the peptidoglycan layer. The electron microscopy structure of the TssJLM complex revealed that it has a diameter of ~18 nm in the periplasm, which is larger than the size of peptidoglycan pores (~2 nm), hence questioning how the T6SS membrane complex crosses the peptidoglycan layer...
January 2017: EMBO Reports
https://www.readbyqxmd.com/read/27871130/paar-rhs-proteins-harbor-various-c-terminal-toxins-to-diversify-the-antibacterial-pathways-of-type-vi-secretion-systems
#7
Jiale Ma, Min Sun, Wenyang Dong, Zihao Pan, Chengping Lu, Huochun Yao
The type VI secretion system (T6SS) of bacteria plays a key role in competing for specific niches by the contact-dependent killing of competitors. Recently, Rhs proteins with polymorphic C-terminal toxin-domains that inhibit or kill neighboring cells were identified. In this report, we identified a novel Rhs with an MPTase4 (Metallopeptidase-4) domain (designated as Rhs-CT1) that showed an antibacterial effect via T6SS in Escherichia coli. We managed to develop a specific strategy by matching the diagnostic domain-architecture of Rhs-CT1 (Rhs with an N-terminal PAAR-motif and a C-terminal toxin domain) for effector retrieval and discovered a series of Rhs-CTs in E...
November 21, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27856117/adaptor-proteins-of-type-vi-secretion-system-effectors
#8
Daniel Unterweger, Benjamin Kostiuk, Stefan Pukatzki
Bacteria use the type VI secretion system (T6SS) to kill neighboring cells. One key feature of the T6SS is the secretion of diverse effectors. Here, we discuss six publications that describe three superfamilies of T6SS proteins, each dedicated to mediate the secretion of cognate effectors.
January 2017: Trends in Microbiology
https://www.readbyqxmd.com/read/27845364/a-genomic-island-in-vibrio-cholerae-with-vpi-1-site-specific-recombination-characteristics-contains-crispr-cas-and-type-vi-secretion-modules
#9
Maurizio Labbate, Fabini D Orata, Nicola K Petty, Nathasha D Jayatilleke, William L King, Paul C Kirchberger, Chris Allen, Gulay Mann, Ankur Mutreja, Nicholas R Thomson, Yan Boucher, Ian G Charles
Cholera is a devastating diarrhoeal disease caused by certain strains of serogroup O1/O139 Vibrio cholerae. Mobile genetic elements such as genomic islands (GIs) have been pivotal in the evolution of O1/O139 V. cholerae. Perhaps the most important GI involved in cholera disease is the V. cholerae pathogenicity island 1 (VPI-1). This GI contains the toxin-coregulated pilus (TCP) gene cluster that is necessary for colonization of the human intestine as well as being the receptor for infection by the cholera-toxin bearing CTX phage...
November 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27832588/secreted-effectors-encoded-within-and-outside-of-the-francisella-pathogenicity-island-promote-intramacrophage-growth
#10
Aria Eshraghi, Jungyun Kim, Alexandra C Walls, Hannah E Ledvina, Cheryl N Miller, Kathryn M Ramsey, John C Whitney, Matthew C Radey, S Brook Peterson, Brittany R Ruhland, Bao Q Tran, Young Ah Goo, David R Goodlett, Simon L Dove, Jean Celli, David Veesler, Joseph D Mougous
The intracellular bacterial pathogen Francisella tularensis causes tularemia, a zoonosis that can be fatal. The type VI secretion system (T6SS) encoded by the Francisella pathogenicity island (FPI) is critical for the virulence of this organism. Existing studies suggest that the complete repertoire of T6SS effectors delivered to host cells is encoded by the FPI. Using a proteome-wide approach, we discovered that the FPI-encoded T6SS exports at least three effectors encoded outside of the island. These proteins share features with virulence determinants of other pathogens, and we provide evidence that they can contribute to intramacrophage growth...
November 9, 2016: Cell Host & Microbe
https://www.readbyqxmd.com/read/27830989/a-mutagenesis-based-approach-identifies-amino-acids-in-the-n-terminal-part-of-francisella-tularensis-igle-that-critically-control-type-vi-system-mediated-secretion
#11
Jeanette E Bröms, Lena Meyer, Anders Sjöstedt
The Gram-negative bacterium Francisella tularensis is the etiological agent of the zoonotic disease tularemia. Its life cycle is characterized by an ability to survive within phagocytic cells through phagosomal escape and replication in the cytosol, ultimately causing inflammasome activation and host cell death. Required for these processes is the Francisella Pathogenicity Island (FPI), which encodes a Type VI secretion system (T6SS) that is active during intracellular infection. In this study, we analyzed the role of the FPI-component IglE, a lipoprotein which we previously have shown to be secreted in a T6SS-dependent manner...
November 10, 2016: Virulence
https://www.readbyqxmd.com/read/27810946/bacterial-secretion-systems-and-regulation-of-inflammasome-activation
#12
REVIEW
Dmitry Ratner, M Pontus A Orning, Egil Lien
Innate immunity is critical for host defenses against pathogens, but many bacteria display complex ways of interacting with innate immune signaling, as they may both activate and evade certain pathways. Gram-negative bacteria can exhibit specialized nanomachine secretion systems for delivery of effector proteins into mammalian cells. Bacterial types III, IV, and VI secretion systems (T3SS, T4SS, and T6SS) are known for their impact on caspase-1-activating inflammasomes, necessary for producing bioactive inflammatory cytokines IL-1β and IL-18, key participants of anti-bacterial responses...
January 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/27803692/classification-of-a-hypervirulent-aeromonas-hydrophila-pathotype-responsible-for-epidemic-outbreaks-in-warm-water-fishes
#13
Cody R Rasmussen-Ivey, Mohammad J Hossain, Sara E Odom, Jeffery S Terhune, William G Hemstreet, Craig A Shoemaker, Dunhua Zhang, De-Hai Xu, Matt J Griffin, Yong-Jie Liu, Maria J Figueras, Scott R Santos, Joseph C Newton, Mark R Liles
Lineages of hypervirulent Aeromonas hydrophila (vAh) are the cause of persistent outbreaks of motile Aeromonas septicemia in warm-water fishes worldwide. Over the last decade, this virulent lineage of A. hydrophila has resulted in annual losses of millions of tons of farmed carp and catfish in the People's Republic of China and the United States (US). Multiple lines of evidence indicate US catfish and Asian carp isolates of A. hydrophila affiliated with sequence type 251 (ST251) share a recent common ancestor...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27799332/ph-alkalinization-by-chloroquine-suppresses-pathogenic-burkholderia-type-6-secretion-system-1-and-multinucleated-giant-cells
#14
Jennifer Chua, Jeffrey L Senft, Stephen J Lockett, Paul J Brett, Mary N Burtnick, David DeShazer, Arthur M Friedlander
Burkholderia mallei and B. pseudomallei cause glanders and melioidosis, respectively, in humans and animals. A hallmark of pathogenesis is the formation of granulomas containing multinucleated giant cells (MNGCs) and cell death. These processes depend on type 6 secretion system 1 (T6SS-1), which is required for virulence in animals. We examined the cell biology of MNGC formation and cell death. We found that chloroquine diphosphate (CLQ), an antimalarial drug, inhibits Burkholderia growth, phagosomal escape, and subsequent MNGC formation...
January 2017: Infection and Immunity
https://www.readbyqxmd.com/read/27799293/global-dynamic-proteome-study-of-a-pellicle-forming-acinetobacter-baumannii-strain
#15
Takfarinas Kentache, Ahmed Ben Abdelkrim, Thierry Jouenne, Emmanuelle Dé, Julie Hardouin
For several decades, many bacteria, among which A. baumannii, have shown their ability to colonize the upper surface of static liquids, forming a biofilm at the air-liquid interface named pellicle. Despite the ubiquity of these pellicles in both natural and artificial environments, few studies have investigated this biofilm type. The present data set provides the first description of the whole proteome of A. baumannii cells grown as pellicle, using a label-free mass spectrometry approach. Results are in accord with the general findings reporting that sessile bacteria are far more resistant to detrimental conditions than their planktonic counterparts, by the accumulation of stress proteins...
January 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/27777222/comprehensive-assessment-and-performance-improvement-of-effector-protein-predictors-for-bacterial-secretion-systems-iii-iv-and-vi
#16
Yi An, Jiawei Wang, Chen Li, André Leier, Tatiana Marquez-Lago, Jonathan Wilksch, Yang Zhang, Geoffrey I Webb, Jiangning Song, Trevor Lithgow
Bacterial effector proteins secreted by various protein secretion systems play crucial roles in host-pathogen interactions. In this context, computational tools capable of accurately predicting effector proteins of the various types of bacterial secretion systems are highly desirable. Existing computational approaches use different machine learning (ML) techniques and heterogeneous features derived from protein sequences and/or structural information. These predictors differ not only in terms of the used ML methods but also with respect to the used curated data sets, the features selection and their prediction performance...
October 24, 2016: Briefings in Bioinformatics
https://www.readbyqxmd.com/read/27729508/genetic-dissection-of-the-type-vi-secretion-system-in-acinetobacter-and-identification-of-a-novel-peptidoglycan-hydrolase-tagx-required-for-its-biogenesis
#17
Brent S Weber, Seth W Hennon, Meredith S Wright, Nichollas E Scott, Véronique de Berardinis, Leonard J Foster, Juan A Ayala, Mark D Adams, Mario F Feldman
: The type VI secretion system (T6SS) is a widespread secretory apparatus produced by Gram-negative bacteria that has emerged as a potent mediator of antibacterial activity during interbacterial interactions. Most Acinetobacter species produce a genetically conserved T6SS, although the expression and functionality of this system vary among different strains. Some pathogenic Acinetobacter baumannii strains activate this secretion system via the spontaneous loss of a plasmid carrying T6SS repressors...
October 11, 2016: MBio
https://www.readbyqxmd.com/read/27698444/structure-and-specificity-of-the-type-vi-secretion-system-clpv-tssc-interaction-in-enteroaggregative-escherichia-coli
#18
Badreddine Douzi, Yannick R Brunet, Silvia Spinelli, Valentine Lensi, Pierre Legrand, Stéphanie Blangy, Anant Kumar, Laure Journet, Eric Cascales, Christian Cambillau
The Type VI secretion system (T6SS) is a versatile machine that delivers toxins into either eukaryotic or bacterial cells. It thus represents a key player in bacterial pathogenesis and inter-bacterial competition. Schematically, the T6SS can be viewed as a contractile tail structure anchored to the cell envelope. The contraction of the tail sheath propels the inner tube loaded with effectors towards the target cell. The components of the contracted tail sheath are then recycled by the ClpV AAA(+) ATPase for a new cycle of tail elongation...
October 4, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27637882/microbial-herd-protection-mediated-by-antagonistic-interaction-in-polymicrobial-communities
#19
Megan Wong, Xiaoye Liang, Matt Smart, Le Tang, Richard Moore, Brian Ingalls, Tao G Dong
: In the host and natural environments, microbes often exist in complex multispecies communities. The molecular mechanisms through which such communities develop and persist - despite significant antagonistic interactions between species - are not well understood. The type VI secretion system (T6SS) is a lethal weapon commonly employed by Gram-negative bacteria to inhibit neighboring species through delivery of toxic effectors. It is well established that intra-species protection is conferred by immunity proteins that neutralize effector toxicities...
September 16, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27616061/type-vi-secretion-system-substrates-are-transferred-and-reused-among-sister-cells
#20
Andrea Vettiger, Marek Basler
Bacterial type VI secretion system (T6SS) is a nanomachine that works similarly to a speargun. Rapid contraction of a sling (sheath) drives a long shaft (Hcp) with a sharp tip and associated effectors through the target cell membrane. We show that the amount and composition of the tip regulates initiation of full-length sheath assembly and low amount of available Hcp decreases sheath length. Importantly, we show that both tip and Hcp are exchanged by T6SS among by-standing cells within minutes of initial cell-cell contact...
September 22, 2016: Cell
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