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https://www.readbyqxmd.com/read/27920034/domestication-of-a-housekeeping-transglycosylase-for-assembly-of-a-type-vi-secretion-system
#1
Yoann G Santin, Eric Cascales
The type VI secretion system (T6SS) is an anti-bacterial weapon comprising a contractile tail anchored to the cell envelope by a membrane complex. The TssJ, TssL, and TssM proteins assemble a 1.7-MDa channel complex that spans the cell envelope, including the peptidoglycan layer. The electron microscopy structure of the TssJLM complex revealed that it has a diameter of ~18 nm in the periplasm, which is larger than the size of peptidoglycan pores (~2 nm), hence questioning how the T6SS membrane complex crosses the peptidoglycan layer...
December 5, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27871130/paar-rhs-proteins-harbor-various-c-terminal-toxins-to-diversify-the-antibacterial-pathways-of-type-vi-secretion-systems
#2
Jiale Ma, Min Sun, Wengyang Dong, Zihao Pan, Chengping Lu, Huochun Yao
The type VI secretion system (T6SS) of bacteria plays a key role in competing for specific niches by the contact-dependent killing of competitors. Recently, Rhs proteins with polymorphic C-terminal toxin-domains that inhibit or kill neighboring cells were identified. In this report, we identified a novel Rhs with an MPTase4 (Metallopeptidase-4) domain (designated as Rhs-CT1) that showed an antibacterial effect via T6SS in Escherichia coli. We managed to develop a specific strategy by matching the diagnostic domain-architecture of Rhs-CT1 (Rhs with an N-terminal PAAR-motif and a C-terminal toxin domain) for effector retrieval and discovered a series of Rhs-CTs in E...
November 21, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27856117/adaptor-proteins-of-type-vi-secretion-system-effectors
#3
Daniel Unterweger, Benjamin Kostiuk, Stefan Pukatzki
Bacteria use the type VI secretion system (T6SS) to kill neighboring cells. One key feature of the T6SS is the secretion of diverse effectors. Here, we discuss six publications that describe three superfamilies of T6SS proteins, each dedicated to mediate the secretion of cognate effectors.
November 14, 2016: Trends in Microbiology
https://www.readbyqxmd.com/read/27845364/a-genomic-island-in-vibrio-cholerae-with-vpi-1-site-specific-recombination-characteristics-contains-crispr-cas-and-type-vi-secretion-modules
#4
Maurizio Labbate, Fabini D Orata, Nicola K Petty, Nathasha D Jayatilleke, William L King, Paul C Kirchberger, Chris Allen, Gulay Mann, Ankur Mutreja, Nicholas R Thomson, Yan Boucher, Ian G Charles
Cholera is a devastating diarrhoeal disease caused by certain strains of serogroup O1/O139 Vibrio cholerae. Mobile genetic elements such as genomic islands (GIs) have been pivotal in the evolution of O1/O139 V. cholerae. Perhaps the most important GI involved in cholera disease is the V. cholerae pathogenicity island 1 (VPI-1). This GI contains the toxin-coregulated pilus (TCP) gene cluster that is necessary for colonization of the human intestine as well as being the receptor for infection by the cholera-toxin bearing CTX phage...
November 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27832588/secreted-effectors-encoded-within-and-outside-of-the-francisella-pathogenicity-island-promote-intramacrophage-growth
#5
Aria Eshraghi, Jungyun Kim, Alexandra C Walls, Hannah E Ledvina, Cheryl N Miller, Kathryn M Ramsey, John C Whitney, Matthew C Radey, S Brook Peterson, Brittany R Ruhland, Bao Q Tran, Young Ah Goo, David R Goodlett, Simon L Dove, Jean Celli, David Veesler, Joseph D Mougous
The intracellular bacterial pathogen Francisella tularensis causes tularemia, a zoonosis that can be fatal. The type VI secretion system (T6SS) encoded by the Francisella pathogenicity island (FPI) is critical for the virulence of this organism. Existing studies suggest that the complete repertoire of T6SS effectors delivered to host cells is encoded by the FPI. Using a proteome-wide approach, we discovered that the FPI-encoded T6SS exports at least three effectors encoded outside of the island. These proteins share features with virulence determinants of other pathogens, and we provide evidence that they can contribute to intramacrophage growth...
November 9, 2016: Cell Host & Microbe
https://www.readbyqxmd.com/read/27830989/a-mutagenesis-based-approach-identifies-amino-acids-in-the-n-terminal-part-of-francisella-tularensis-igle-that-critically-control-type-vi-system-mediated-secretion
#6
Jeanette E Bröms, Lena Meyer, Anders Sjöstedt
The Gram-negative bacterium Francisella tularensis is the etiological agent of the zoonotic disease tularemia. Its life cycle is characterized by an ability to survive within phagocytic cells through phagosomal escape and replication in the cytosol, ultimately causing inflammasome activation and host cell death. Required for these processes is the Francisella pathogenicity island (FPI), which encodes a Type VI secretion system (T6SS) that is active during intracellular infection. In this study, we analyzed the role of the FPI-component IglE, a lipoprotein which we previously have shown to be secreted in a T6SS-dependent manner...
November 10, 2016: Virulence
https://www.readbyqxmd.com/read/27810946/bacterial-secretion-systems-and-regulation-of-inflammasome-activation
#7
REVIEW
Dmitry Ratner, M Pontus A Orning, Egil Lien
Innate immunity is critical for host defenses against pathogens, but many bacteria display complex ways of interacting with innate immune signaling, as they may both activate and evade certain pathways. Gram-negative bacteria can exhibit specialized nanomachine secretion systems for delivery of effector proteins into mammalian cells. Bacterial types III, IV, and VI secretion systems (T3SS, T4SS, and T6SS) are known for their impact on caspase-1-activating inflammasomes, necessary for producing bioactive inflammatory cytokines IL-1β and IL-18, key participants of anti-bacterial responses...
November 3, 2016: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/27803692/classification-of-a-hypervirulent-aeromonas-hydrophila-pathotype-responsible-for-epidemic-outbreaks-in-warm-water-fishes
#8
Cody R Rasmussen-Ivey, Mohammad J Hossain, Sara E Odom, Jeffery S Terhune, William G Hemstreet, Craig A Shoemaker, Dunhua Zhang, De-Hai Xu, Matt J Griffin, Yong-Jie Liu, Maria J Figueras, Scott R Santos, Joseph C Newton, Mark R Liles
Lineages of hypervirulent Aeromonas hydrophila (vAh) are the cause of persistent outbreaks of motile Aeromonas septicemia in warm-water fishes worldwide. Over the last decade, this virulent lineage of A. hydrophila has resulted in annual losses of millions of tons of farmed carp and catfish in the People's Republic of China and the United States (US). Multiple lines of evidence indicate US catfish and Asian carp isolates of A. hydrophila affiliated with sequence type 251 (ST251) share a recent common ancestor...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27799332/ph-alkalinization-by-chloroquine-suppresses-pathogenic-burkholderia-t6ss-1-and-multi-nucleated-giant-cells
#9
Jennifer Chua, Jeffrey L Senft, Stephen J Lockett, Paul J Brett, Mary N Burtnick, David DeShazer, Arthur M Friedlander
Burkholderia mallei and B. pseudomallei cause glanders and melioidosis, respectively, in humans and animals. A hallmark of pathogenesis is formation of granulomas containing multi-nucleated giant cells (MNGCs) and cell death. These processes depend on the Type 6 secretion system-1 (T6SS-1), which is required for virulence in animals. We examined the cell biology of MNGC formation and cell death. We found that chloroquine (CLQ), an anti-malarial drug, inhibits Burkholderia growth, phagosomal escape and subsequent MNGC formation...
October 31, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27799293/global-dynamic-proteome-study-of-a-pellicle-forming-acinetobacter-baumannii-strain
#10
Takfarinas Kentache, Ahmed Ben Abdelkrim, Thierry Jouenne, Emmanuelle De, Julie Hardouin
Since several decades, many bacteria, among which A. baumannii, have shown their ability to colonize the upper surface of static liquids, forming a biofilm at the air-liquid interface named pellicle. Despite the ubiquity of these pellicles in both natural and artificial environments, few studies have investigated this biofilm type. The present data set provides the first description of the whole proteome of A. baumannii cells grown as pellicle, using a label-free mass spectrometry approach. Results accord with the general findings reporting that sessile bacteria are far more resistant to detrimental conditions than their planktonic counterparts, by the accumulation of stress proteins...
October 31, 2016: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/27777222/comprehensive-assessment-and-performance-improvement-of-effector-protein-predictors-for-bacterial-secretion-systems-iii-iv-and-vi
#11
Yi An, Jiawei Wang, Chen Li, André Leier, Tatiana Marquez-Lago, Jonathan Wilksch, Yang Zhang, Geoffrey I Webb, Jiangning Song, Trevor Lithgow
Bacterial effector proteins secreted by various protein secretion systems play crucial roles in host-pathogen interactions. In this context, computational tools capable of accurately predicting effector proteins of the various types of bacterial secretion systems are highly desirable. Existing computational approaches use different machine learning (ML) techniques and heterogeneous features derived from protein sequences and/or structural information. These predictors differ not only in terms of the used ML methods but also with respect to the used curated data sets, the features selection and their prediction performance...
October 24, 2016: Briefings in Bioinformatics
https://www.readbyqxmd.com/read/27729508/genetic-dissection-of-the-type-vi-secretion-system-in-acinetobacter-and-identification-of-a-novel-peptidoglycan-hydrolase-tagx-required-for-its-biogenesis
#12
Brent S Weber, Seth W Hennon, Meredith S Wright, Nichollas E Scott, Véronique de Berardinis, Leonard J Foster, Juan A Ayala, Mark D Adams, Mario F Feldman
: The type VI secretion system (T6SS) is a widespread secretory apparatus produced by Gram-negative bacteria that has emerged as a potent mediator of antibacterial activity during interbacterial interactions. Most Acinetobacter species produce a genetically conserved T6SS, although the expression and functionality of this system vary among different strains. Some pathogenic Acinetobacter baumannii strains activate this secretion system via the spontaneous loss of a plasmid carrying T6SS repressors...
October 11, 2016: MBio
https://www.readbyqxmd.com/read/27698444/structure-and-specificity-of-the-type-vi-secretion-system-clpv-tssc-interaction-in-enteroaggregative-escherichia-coli
#13
Badreddine Douzi, Yannick R Brunet, Silvia Spinelli, Valentine Lensi, Pierre Legrand, Stéphanie Blangy, Anant Kumar, Laure Journet, Eric Cascales, Christian Cambillau
The Type VI secretion system (T6SS) is a versatile machine that delivers toxins into either eukaryotic or bacterial cells. It thus represents a key player in bacterial pathogenesis and inter-bacterial competition. Schematically, the T6SS can be viewed as a contractile tail structure anchored to the cell envelope. The contraction of the tail sheath propels the inner tube loaded with effectors towards the target cell. The components of the contracted tail sheath are then recycled by the ClpV AAA(+) ATPase for a new cycle of tail elongation...
October 4, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27637882/microbial-herd-protection-mediated-by-antagonistic-interaction-in-polymicrobial-communities
#14
Megan Wong, Xiaoye Liang, Matt Smart, Le Tang, Richard Moore, Brian Ingalls, Tao G Dong
: In the host and natural environments, microbes often exist in complex multispecies communities. The molecular mechanisms through which such communities develop and persist - despite significant antagonistic interactions between species - are not well understood. The type VI secretion system (T6SS) is a lethal weapon commonly employed by Gram-negative bacteria to inhibit neighboring species through delivery of toxic effectors. It is well established that intra-species protection is conferred by immunity proteins that neutralize effector toxicities...
September 16, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27616061/type-vi-secretion-system-substrates-are-transferred-and-reused-among-sister-cells
#15
Andrea Vettiger, Marek Basler
Bacterial type VI secretion system (T6SS) is a nanomachine that works similarly to a speargun. Rapid contraction of a sling (sheath) drives a long shaft (Hcp) with a sharp tip and associated effectors through the target cell membrane. We show that the amount and composition of the tip regulates initiation of full-length sheath assembly and low amount of available Hcp decreases sheath length. Importantly, we show that both tip and Hcp are exchanged by T6SS among by-standing cells within minutes of initial cell-cell contact...
September 22, 2016: Cell
https://www.readbyqxmd.com/read/27605490/involvement-of-type-vi-secretion-system-in-secretion-of-iron-chelator-pyoverdine-in-pseudomonas-taiwanensis
#16
Wen-Jen Chen, Tzu-Yen Kuo, Feng-Chia Hsieh, Pi-Yu Chen, Chang-Sheng Wang, Yu-Ling Shih, Ying-Mi Lai, Je-Ruei Liu, Yu-Liang Yang, Ming-Che Shih
Rice bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive rice diseases worldwide. Therefore, in addition to breeding disease-resistant rice cultivars, it is desirable to develop effective biocontrol agents against Xoo. Here, we report that a soil bacterium Pseudomonas taiwanensis displayed strong antagonistic activity against Xoo. Using matrix-assisted laser desorption/ionization imaging mass spectrometry, we identified an iron chelator, pyoverdine, secreted by P. taiwanensis that could inhibit the growth of Xoo...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27602570/francisella-tularensis-iglg-belongs-to-a-novel-family-of-paar-like-t6ss-proteins-and-harbors-a-unique-n-terminal-extension-required-for-virulence
#17
Mélanie Rigard, Jeanette E Bröms, Amandine Mosnier, Maggy Hologne, Amandine Martin, Lena Lindgren, Claire Punginelli, Claire Lays, Olivier Walker, Alain Charbit, Philippe Telouk, Wayne Conlan, Laurent Terradot, Anders Sjöstedt, Thomas Henry
The virulence of Francisella tularensis, the etiological agent of tularemia, relies on an atypical type VI secretion system (T6SS) encoded by a genomic island termed the Francisella Pathogenicity Island (FPI). While the importance of the FPI in F. tularensis virulence is clearly established, the precise role of most of the FPI-encoded proteins remains to be deciphered. In this study, using highly virulent F. tularensis strains and the closely related species F. novicida, IglG was characterized as a protein featuring a unique α-helical N-terminal extension and a domain of unknown function (DUF4280), present in more than 250 bacterial species...
September 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27602025/relevant-genes-linked-to-virulence-are-required-for-salmonella-typhimurium-to-survive-intracellularly-in-the-social-amoeba-dictyostelium-discoideum
#18
Sebastián Riquelme, Macarena Varas, Camila Valenzuela, Paula Velozo, Nicolás Chahin, Paulina Aguilera, Andrea Sabag, Bayron Labra, Sergio A Álvarez, Francisco P Chávez, Carlos A Santiviago
The social amoeba Dictyostelium discoideum has proven to be a useful model for studying relevant aspects of the host-pathogen interaction. In this work, D. discoideum was used as a model to study the ability of Salmonella Typhimurium to survive in amoebae and to evaluate the contribution of selected genes in this process. To do this, we performed infection assays using axenic cultures of D. discoideum co-cultured with wild-type S. Typhimurium and/or defined mutant strains. Our results confirmed that wild-type S...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27600411/molecular-dissection-of-the-interface-between-the-type-vi-secretion-tssm-cytoplasmic-domain-and-the-tssg-baseplate-component
#19
Laureen Logger, Marie-Stéphanie Aschtgen, Marie Guérin, Eric Cascales, Eric Durand
The type VI secretion system (T6SS) is a multi-protein complex that catalyses toxin secretion through the bacterial cell envelope of various Gram-negative bacteria including important human pathogens. This machine uses a bacteriophage-like contractile tail to puncture the prey cell and inject armful toxins. The T6SS tail comprises an inner tube capped by the cell-puncturing spike and wrapped by the contractile sheath. This structure is built on an assembly platform, the baseplate, which is anchored to the bacterial cell envelope by the TssJLM membrane complex...
September 3, 2016: Journal of Molecular Biology
https://www.readbyqxmd.com/read/27600409/structure-function-analysis-of-the-tssl-cytoplasmic-domain-reveals-a-new-interaction-between-the-type-vi-secretion-baseplate-and-membrane-complexes
#20
Abdelrahim Zoued, Chloé J Cassaro, Eric Durand, Badreddine Douzi, Alexandre P España, Christian Cambillau, Laure Journet, Eric Cascales
The Type VI secretion system (T6SS) is a multiprotein complex that delivers toxin effectors in both prokaryotic and eukaryotic cells. It is constituted of a long cytoplasmic structure - the tail - made of stacked Hcp hexamers and wrapped by a contractile sheath. Contraction of the sheath propels the inner tube capped by the VgrG spike protein towards the target cell. This tubular structure is built onto an assembly platform - the baseplate - that is composed of the TssEFGK-VgrG subunits. During the assembly process, the baseplate is recruited to a trans-envelope complex comprising the TssJ outer membrane lipoprotein and the TssL and TssM inner membrane proteins...
September 3, 2016: Journal of Molecular Biology
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