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https://www.readbyqxmd.com/read/28327641/sequential-displacement-of-type-vi-secretion-system-effector-genes-leads-to-evolution-of-diverse-immunity-gene-arrays-in-vibrio-cholerae
#1
Paul C Kirchberger, Daniel Unterweger, Daniele Provenzano, Stefan Pukatzki, Yan Boucher
Type VI secretion systems (T6SS) enable bacteria to engage neighboring cells in contact-dependent competition. In Vibrio cholerae, three chromosomal clusters each encode a pair of effector and immunity genes downstream of those encoding the T6SS structural machinery for effector delivery. Different combinations of effector-immunity proteins lead to competition between strains of V. cholerae, which are thought to be protected only from the toxicity of their own effectors. Screening of all publically available V...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28288207/type-vi-secretion-system-contributes-to-enterohemorrhagic-escherichia-coli-virulence-by-secreting-catalase-against-host-reactive-oxygen-species-ros
#2
Baoshan Wan, Qiufen Zhang, Jinjing Ni, Shuxian Li, Donghua Wen, Jun Li, Haihan Xiao, Ping He, Hong-Yu Ou, Jing Tao, Qihui Teng, Jie Lu, Wenjuan Wu, Yu-Feng Yao
Enterohemorrhagic Escherichia coli (EHEC) is one major type of contagious and foodborne pathogens. The type VI secretion system (T6SS) has been shown to be involved in the bacterial pathogenicity and bacteria-bacteria competition. Here, we show that EHEC could secrete a novel effector KatN, a Mn-containing catalase, in a T6SS-dependent manner. Expression of katN is promoted by RpoS and OxyR and repressed by H-NS, and katN contributes to bacterial growth under oxidative stress in vitro. KatN could be secreted into host cell cytosol after EHEC is phagocytized by macrophage, which leads to decreased level of intracellular reactive oxygen species (ROS) and facilitates the intramacrophage survival of EHEC...
March 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28281200/zntr-positively-regulates-t6ss4-expression-in-yersinia-pseudotuberculosis
#3
Tietao Wang, Keqi Chen, Fen Gao, Yiwen Kang, Muhammad Tausif Chaudhry, Zhuo Wang, Yao Wang, Xihui Shen
The type VI secretion system (T6SS) is a widespread and versatile protein secretion system found in most Gramnegative bacteria. Studies of T6SS have mainly focused on its role in virulence toward host cells and inter-bacterial interactions, but studies have also shown that T6SS4 in Yersinia pseudotuberculosis participates in the acquisition of zinc ions to alleviate the accumulation of hydroxyl radicals induced by multiple stressors. Here, by comparing the gene expression patterns of wild-type and zntR mutant Y...
March 10, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28277899/don-t-judge-a-book-by-its-cover-the-hcps-are-not-only-structural-components-of-the-t6ss-machinery
#4
Sophie Bleves
No abstract text is available yet for this article.
March 1, 2017: Virulence
https://www.readbyqxmd.com/read/28264992/crispr-cas-and-contact-dependent-secretion-systems-present-on-excisable-pathogenicity-islands-with-conserved-recombination-modules
#5
Megan R Carpenter, Sai S Kalburge, Joseph D Borowski, Molly C Peters, Rita R Colwell, E Fidelma Boyd
Pathogenicity islands (PAIs) are mobile integrated genetic elements that contain a diverse range of virulence factors. PAIs integrate into the host chromosome at a tRNA locus that contains their specific bacterial attachment site, attB, via integrase mediated site-specific recombination generating attL and attR sites. We identified conserved recombination modules (integrases and att sites) previously described in choleragenic V. cholerae PAIs but with novel cargo genes. Clustered regularly interspaced short palindromic repeat (CRISPR)-associated proteins (Cas proteins) and a type VI secretion system (T6SS) gene cluster were identified at the Vibrio Pathogenicity Island-1 (VPI-1) insertion site in nineteen V...
March 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28242693/manganese-scavenging-and-oxidative-stress-response-mediated-by-type-vi-secretion-system-in-burkholderia-thailandensis
#6
Meiru Si, Chao Zhao, Brianne Burkinshaw, Bing Zhang, Dawei Wei, Yao Wang, Tao G Dong, Xihui Shen
Type VI secretion system (T6SS) is a versatile protein export machinery widely distributed in Gram-negative bacteria. Known to translocate protein substrates to eukaryotic and prokaryotic target cells to cause cellular damage, the T6SS has been primarily recognized as a contact-dependent bacterial weapon for microbe-host and microbial interspecies competition. Here we report contact-independent functions of the T6SS for metal acquisition, bacteria competition, and resistance to oxidative stress. We demonstrate that the T6SS-4 in Burkholderia thailandensis is critical for survival under oxidative stress and is regulated by OxyR, a conserved oxidative stress regulator...
February 27, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28236274/comparative-pan-genome-analysis-of-oral-prevotella-species-implicated-in-periodontitis
#7
Maziya Ibrahim, Ahalyaa Subramanian, Sharmila Anishetty
Prevotella is part of the oral bacterial community implicated in periodontitis. Pan genome analyses of eight oral Prevotella species, P. dentalis, P. enoeca, P. fusca, P. melaninogenica, P. denticola, P. intermedia 17, P. intermedia 17-2 and P. sp. oral taxon 299 are presented in this study. Analysis of the Prevotella pan genome revealed features such as secretion systems, resistance to oxidative stress and clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems that enable the bacteria to adapt to the oral environment...
February 24, 2017: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/28217442/whole-genome-sequencing-and-analysis-of-campylobacter-coli-yh502-from-retail-chicken-reveals-a-plasmid-borne-type-vi-secretion-system
#8
Sandeep Ghatak, Yiping He, Sue Reed, Terence Strobaugh, Peter Irwin
Campylobacter is a major cause of foodborne illnesses worldwide. Campylobacter infections, commonly caused by ingestion of undercooked poultry and meat products, can lead to gastroenteritis and chronic reactive arthritis in humans. Whole genome sequencing (WGS) is a powerful technology that provides comprehensive genetic information about bacteria and is increasingly being applied to study foodborne pathogens: e.g., evolution, epidemiology/outbreak investigation, and detection. Herein we report the complete genome sequence of Campylobacter coli strain YH502 isolated from retail chicken in the United States...
March 2017: Genomics Data
https://www.readbyqxmd.com/read/28216293/the-secrets-of-acinetobacter-secretion
#9
REVIEW
Brent S Weber, Rachel L Kinsella, Christian M Harding, Mario F Feldman
Infections caused by the bacterial pathogen Acinetobacter baumannii are a mounting concern for healthcare practitioners as widespread antibiotic resistance continues to limit therapeutic treatment options. The biological processes used by A. baumannii to cause disease are not well defined, but recent research has indicated that secreted proteins may play a major role. A variety of mechanisms have now been shown to contribute to protein secretion by A. baumannii and other pathogenic species of Acinetobacter, including a type II secretion system (T2SS), a type VI secretion system (T6SS), autotransporter, and outer membrane vesicles (OMVs)...
February 16, 2017: Trends in Microbiology
https://www.readbyqxmd.com/read/28165005/killing-by-type-vi-secretion-drives-genetic-phase-separation-and-correlates-with-increased-cooperation
#10
Luke McNally, Eryn Bernardy, Jacob Thomas, Arben Kalziqi, Jennifer Pentz, Sam P Brown, Brian K Hammer, Peter J Yunker, William C Ratcliff
By nature of their small size, dense growth and frequent need for extracellular metabolism, microbes face persistent public goods dilemmas. Genetic assortment is the only general solution stabilizing cooperation, but all known mechanisms structuring microbial populations depend on the availability of free space, an often unrealistic constraint. Here we describe a class of self-organization that operates within densely packed bacterial populations. Through mathematical modelling and experiments with Vibrio cholerae, we show how killing adjacent competitors via the Type VI secretion system (T6SS) precipitates phase separation via the 'Model A' universality class of order-disorder transition mediated by killing...
February 6, 2017: Nature Communications
https://www.readbyqxmd.com/read/28114423/contribution-of-the-pseudomonas-fluorescens-mfe01-type-vi-secretion-system-to-biofilm-formation
#11
Mathias Gallique, Victorien Decoin, Corinne Barbey, Thibaut Rosay, Marc G J Feuilloley, Nicole Orange, Annabelle Merieau
Type VI secretion systems (T6SSs) are widespread in Gram-negative bacteria, including Pseudomonas. These macromolecular machineries inject toxins directly into prokaryotic or eukaryotic prey cells. Hcp proteins are structural components of the extracellular part of this machinery. We recently reported that MFE01, an avirulent strain of Pseudomonas fluorescens, possesses at least two hcp genes, hcp1 and hcp2, encoding proteins playing important roles in interbacterial interactions. Indeed, P. fluorescens MFE01 can immobilise and kill diverse bacteria of various origins through the action of the Hcp1 or Hcp2 proteins of the T6SS...
2017: PloS One
https://www.readbyqxmd.com/read/28112271/secretepdb-a-comprehensive-web-based-resource-for-secreted-effector-proteins-of-the-bacterial-types-iii-iv-and-vi-secretion-systems
#12
Yi An, Jiawei Wang, Chen Li, Jerico Revote, Yang Zhang, Thomas Naderer, Morihiro Hayashida, Tatsuya Akutsu, Geoffrey I Webb, Trevor Lithgow, Jiangning Song
Bacteria translocate effector molecules to host cells through highly evolved secretion systems. By definition, the function of these effector proteins is to manipulate host cell biology and the sequence, structural and functional annotations of these effector proteins will provide a better understanding of how bacterial secretion systems promote bacterial survival and virulence. Here we developed a knowledgebase, termed SecretEPDB (Bacterial Secreted Effector Protein DataBase), for effector proteins of type III secretion system (T3SS), type IV secretion system (T4SS) and type VI secretion system (T6SS)...
January 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28081443/the-bacterial-t6ss-effector-evpp-prevents-nlrp3-inflammasome-activation-by-inhibiting-the-ca-2-dependent-mapk-jnk-pathway
#13
Hao Chen, Dahai Yang, Fajun Han, Jinchao Tan, Lingzhi Zhang, Jingfan Xiao, Yuanxing Zhang, Qin Liu
Inflammasome activation is an important innate immune defense mechanism against bacterial infection, and in return, bacteria express virulence determinants that counteract inflammasome activation. Many such effectors are secreted into host cells via specialized bacterial secretion systems. Here, the intracellular pathogenic bacterium Edwardsiella tarda was demonstrated to activate NLRC4 and NLRP3 inflammasomes via a type III secretion system (T3SS), and to inhibit NLRP3 inflammasome via a type VI secretion system (T6SS), indicating the antagonistic roles of these systems in inflammasome signaling...
January 11, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28060574/the-hcp-proteins-fused-with-diverse-extended-toxin-domains-represent-a-novel-pattern-of-antibacterial-effectors-in-type-vi-secretion-systems
#14
Jiale Ma, Zihao Pan, Jinhu Huang, Min Sun, Chengping Lu, Huochun Yao
The type VI secretion system (T6SS) is a widespread molecular weapon deployed by many bacterial species to target eukaryotic host cells or rival bacteria. Using a dynamic injection mechanism, diverse effectors can be delivered by T6SS directly into recipient cells. Here, we report a new family of T6SS effectors encoded by extended Hcps carrying diverse toxin domains. Bioinformatic analyses revealed that these Hcps with C-terminal extension toxins, designated as Hcp-ET, exist widely in the Enterobacteriaceae...
January 6, 2017: Virulence
https://www.readbyqxmd.com/read/28045455/the-pseudomonas-putida-t6ss-is-a-plant-warden-against-phytopathogens
#15
Patricia Bernal, Luke P Allsopp, Alain Filloux, María A Llamas
Bacterial type VI secretion systems (T6SSs) are molecular weapons designed to deliver toxic effectors into prey cells. These nanomachines have an important role in inter-bacterial competition and provide advantages to T6SS active strains in polymicrobial environments. Here we analyze the genome of the biocontrol agent Pseudomonas putida KT2440 and identify three T6SS gene clusters (K1-, K2- and K3-T6SS). Besides, 10 T6SS effector-immunity pairs were found, including putative nucleases and pore-forming colicins...
April 2017: ISME Journal
https://www.readbyqxmd.com/read/28027803/rules-of-engagement-the-type-vi-secretion-system-in-vibrio-cholerae
#16
REVIEW
Avatar Joshi, Benjamin Kostiuk, Andrew Rogers, Jennifer Teschler, Stefan Pukatzki, Fitnat H Yildiz
Microbial species often exist in complex communities where they must avoid predation and compete for favorable niches. The type VI secretion system (T6SS) is a contact-dependent bacterial weapon that allows for direct killing of competitors through the translocation of proteinaceous toxins. Vibrio cholerae is a Gram-negative pathogen that can use its T6SS during antagonistic interactions with neighboring prokaryotic and eukaryotic competitors. The T6SS not only promotes V. cholerae's survival during its aquatic and host life cycles, but also influences its evolution by facilitating horizontal gene transfer...
December 24, 2016: Trends in Microbiology
https://www.readbyqxmd.com/read/27974393/temperature-regulated-expression-of-type-vi-secretion-systems-in-fish-pathogen-pseudomonas-plecoglossicida-revealed-by-comparative-secretome-analysis
#17
Zhen Tao, Tao Zhou, Suming Zhou, Guoliang Wang
Pseudomonas plecoglossicida is a facultative fish pathogen. Recent studies showed that P. plecoglossicida infection in fish was associated with temperature. The aim of this study was to compare the secretomes of P. plecoglossicida cultured in vitro at representative temperatures for pathogenic (20°C) and less pathogenic (30°C) phenotypes. Thirteen proteins in the culture supernatants of P. plecoglossicida showed significant difference in abundance at 20 vs. 30°C. Four proteins were strongly increased at 20°C, including two hemolysin co-regulated proteins (Hcp) that are part of the bacterial type VI secretion system (T6SS), flagellin and an unknown protein...
November 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27920034/domestication-of-a-housekeeping-transglycosylase-for-assembly-of-a-type-vi-secretion-system
#18
Yoann G Santin, Eric Cascales
The type VI secretion system (T6SS) is an anti-bacterial weapon comprising a contractile tail anchored to the cell envelope by a membrane complex. The TssJ, TssL, and TssM proteins assemble a 1.7-MDa channel complex that spans the cell envelope, including the peptidoglycan layer. The electron microscopy structure of the TssJLM complex revealed that it has a diameter of ~18 nm in the periplasm, which is larger than the size of peptidoglycan pores (~2 nm), hence questioning how the T6SS membrane complex crosses the peptidoglycan layer...
January 2017: EMBO Reports
https://www.readbyqxmd.com/read/27871130/paar-rhs-proteins-harbor-various-c-terminal-toxins-to-diversify-the-antibacterial-pathways-of-type-vi-secretion-systems
#19
Jiale Ma, Min Sun, Wenyang Dong, Zihao Pan, Chengping Lu, Huochun Yao
The type VI secretion system (T6SS) of bacteria plays a key role in competing for specific niches by the contact-dependent killing of competitors. Recently, Rhs proteins with polymorphic C-terminal toxin-domains that inhibit or kill neighboring cells were identified. In this report, we identified a novel Rhs with an MPTase4 (Metallopeptidase-4) domain (designated as Rhs-CT1) that showed an antibacterial effect via T6SS in Escherichia coli. We managed to develop a specific strategy by matching the diagnostic domain-architecture of Rhs-CT1 (Rhs with an N-terminal PAAR-motif and a C-terminal toxin domain) for effector retrieval and discovered a series of Rhs-CTs in E...
November 21, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27856117/adaptor-proteins-of-type-vi-secretion-system-effectors
#20
Daniel Unterweger, Benjamin Kostiuk, Stefan Pukatzki
Bacteria use the type VI secretion system (T6SS) to kill neighboring cells. One key feature of the T6SS is the secretion of diverse effectors. Here, we discuss six publications that describe three superfamilies of T6SS proteins, each dedicated to mediate the secretion of cognate effectors.
January 2017: Trends in Microbiology
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