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https://www.readbyqxmd.com/read/29147941/artificial-bio-nanomachines-based-on-protein-needles-derived-from-bacteriophage-t4
#1
REVIEW
Hiroshi Inaba, Takafumi Ueno
Bacteriophage T4 is a natural bio-nanomachine which achieves efficient infection of host cells via cooperative motion of specific three-dimensional protein architectures. The relationships between the protein structures and their dynamic functions have recently been clarified. In this review we summarize the design principles for fabrication of nanomachines using the component proteins of bacteriophage T4 based on these recent advances. We focus on the protein needle known as gp5, which is located at the center of the baseplate at the end of the contractile tail of bacteriophage T4...
November 16, 2017: Biophysical Reviews
https://www.readbyqxmd.com/read/29109705/complete-genome-sequence-of-vibrio-campbellii-lmb-29-isolated-from-red-drum-with-four-native-megaplasmids
#2
Jinxin Liu, Zhe Zhao, Yiqing Deng, Yan Shi, Yupeng Liu, Chao Wu, Peng Luo, Chaoqun Hu
Vibrio spp. are the most common pathogens for animals reared in aquaculture. Vibrio campbellii, which is often involved in shrimp, fish and mollusks diseases, is widely distributed in the marine environment worldwide, but our knowledge about its pathogenesis and antimicrobial resistance is very limited. The existence of this knowledge gap is at least partially because that V. campbellii was originally classified as Vibrio harveyi, and the detailed information of its comparative genome analysis to other Vibrio spp...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29090192/identification-of-new-virulence-factors-and-vaccine-candidates-for-yersinia-pestis
#3
Jourdan A Andersson, Jian Sha, Tatiana E Erova, Eric C Fitts, Duraisamy Ponnusamy, Elena V Kozlova, Michelle L Kirtley, Ashok K Chopra
Earlier, we reported the identification of new virulence factors/mechanisms of Yersinia pestis using an in vivo signature-tagged mutagenesis (STM) screening approach. From this screen, the role of rbsA, which encodes an ATP-binding protein of ribose transport system, and vasK, an essential component of the type VI secretion system (T6SS), were evaluated in mouse models of plague and confirmed to be important during Y. pestis infection. However, many of the identified genes from the screen remained uncharacterized...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29085808/identification-and-characterization-of-an-antibacterial-type-vi-secretion-system-in-the-carbapenem-resistant-strain-klebsiella-pneumoniae-hs11286
#4
Lu Liu, Meiping Ye, Xiaobin Li, Jun Li, Zixin Deng, Yu-Feng Yao, Hong-Yu Ou
The type VI secretion system (T6SS) is a class of sophisticated cell contact-dependent apparatus with anti-eukaryotic or anti-bacterial function. Klebsiella pneumoniae is one of the most common bacterial pathogens with resistance to the carbapenem antibiotics. However, little is known about the antibacterial T6SS in K. pneumoniae. Using core-component protein searches, we identified a putative T6SS gene cluster on the chromosome of the carbapenemase-producing K. pneumoniae (CRKP) strain HS11286. Intraspecies and interspecies competition assays revealed an antibacterial function of the HS11286 T6SS...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29031453/whole-genome-comparison-of-urinary-pathogenic-escherichia-coli-and-faecal-isolates-of-uti-patients-and-healthy-controls
#5
Karen Leth Nielsen, Marc Stegger, Kristoffer Kiil, Paul A Godfrey, Michael Feldgarden, Berit Lilje, Paal S Andersen, Niels Frimodt-Møller
The faecal flora is a common reservoir for urinary tract infection (UTI), and Escherichia coli (E. coli) is frequently found in this reservoir without causing extraintestinal infection. We investigated these E. coli reservoirs by whole-genome sequencing a large collection of E. coli from healthy controls (faecal), who had never previously had UTI, and from UTI patients (faecal and urinary) sampled from the same geographical area. We compared MLST types, phylogenetic relationship, accessory genome content and FimH type between patient and control faecal isolates as well as between UTI and faecal-only isolates, respectively...
September 14, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29027348/type-vi-secretion-systems-in-plant-associated-bacteria
#6
Patricia Bernal, María A Llamas, Alain Filloux
The type VI secretion system (T6SS) is a bacterial nanomachine used to inject effectors into prokaryotic or eukaryotic cells and is thus involved in both host manipulation and inter-bacterial competition. The T6SS is widespread among Gram-negative bacteria, mostly within the Proteobacterium Phylum. This secretion system is commonly found in commensal and pathogenic plant-associated bacteria. Phylogenetic analysis of phytobacterial T6SS clusters shows that they are distributed in the five main clades previously described (group 1 to 5)...
October 13, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28979890/structure-based-prototype-peptides-targeting-the-pseudomonas-aeruginosa-type-vi-secretion-system-effector-as-a-novel-antibacterial-strategy
#7
Xiaopan Gao, Zhixia Mu, Bo Qin, Yicheng Sun, Sheng Cui
The type VI secretion system (T6SS) secretes numerous toxins for bacteria-bacteria competition. TplE is a newly identified trans-kingdom toxin secreted by the T6SS in Pseudomonas aeruginosa, while TplEi neutralizes the toxic effect of TplE to protect bacteria autointoxication. Blocking the interaction of TplE-TplEi could unleash the toxin, causing bacterial cell death. In this study, we applied a crystallographic approach to design a structural-based antimicrobial peptides targeting the interaction of TplE and TplEi...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28973030/cholesterol-and-host-cell-surface-proteins-contribute-to-cell-cell-fusion-induced-by-the-burkholderia-type-vi-secretion-system-5
#8
Liam Whiteley, Maria Haug, Kristina Klein, Matthias Willmann, Erwin Bohn, Salvatore Chiantia, Sandra Schwarz
Following escape into the cytoplasm of host cells, Burkholderia pseudomallei and the related species Burkholderia thailandensis employ the type VI secretion system 5 (T6SS-5) to induce plasma membrane fusion with an adjacent host cell. This process leads to the formation of multinucleated giant cells and facilitates bacterial access to an uninfected host cell in a direct manner. Despite its importance in virulence, the mechanism of the T6SS-5 and the role of host cell factors in cell-cell fusion remain elusive...
2017: PloS One
https://www.readbyqxmd.com/read/28961768/new-facet-of-non-o1-non-o139-vibrio-cholerae-hemolysin-a-a-competitive-factor-for-the-environmental-niche
#9
Pichet Ruenchit, Onrapak Reamtong, Kanokrat Siripanichgon, Wanpen Chaicumpa, Pornphan Diraphat
Different serogroups of Vibrio cholerae may inhabit the same ecological niche. However, serogroup O1/O139 strains are rarely isolated from their ecological sources. Quite plausibly, the non-O1/non-O139 vibrios and other bacterial species suppress growth of the O1/O139 that shares the same niche. Our bacterial inhibition assay data indicated that certain non-O1/non-O139 strains used a contact-dependent type VI secretion system (T6SS) to suppress growth of the O1 El Tor, N16961 pandemic strain. Meanwhile, comparative proteomics of the O1 and the suppressive non-O1/non-O139 co-cultured in the simulated natural aquatic microcosm showed that SecB and HlyD were upregulated in the latter...
September 6, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28955357/comparative-genomics-of-ralstonia-solanacearum-identifies-candidate-genes-associated-with-cool-virulence
#10
Ana M Bocsanczy, Jose C Huguet-Tapia, David J Norman
Strains of the Ralstonia solanacearum species complex in the phylotype IIB group are capable of causing Bacterial Wilt disease in potato and tomato at temperatures lower than 24°C. The capability of these strains to survive and to incite infection at temperatures colder than their normally tropical boundaries represents a threat to United States agriculture in temperate regions. In this work, we used a comparative genomics approach to identify orthologous genes linked to the lower temperature virulence phenotype...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28947741/cryo-em-structure-of-the-extended-type-vi-secretion-system-sheath-tube-complex
#11
Jing Wang, Maximilian Brackmann, Daniel Castaño-Díez, Mikhail Kudryashev, Kenneth N Goldie, Timm Maier, Henning Stahlberg, Marek Basler
The bacterial type VI secretion system (T6SS) uses contraction of a long sheath to quickly thrust a tube with associated effectors across membranes of eukaryotic and bacterial cells (1-5) . Only limited structural information is available about the inherently unstable precontraction state of the T6SS. Here, we obtain a 3.7 Å resolution structure of a non-contractile sheath-tube complex using cryo-electron microscopy and show that it resembles the extended T6SS inside Vibrio cholerae cells. We build a pseudo-atomic model of the complete sheath-tube assembly, which provides a mechanistic understanding of coupling sheath contraction with pushing and rotating the inner tube for efficient target membrane penetration...
September 25, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28934168/role-of-the-genes-of-type-vi-secretion-system-in-virulence-of-rice-bacterial-brown-stripe-pathogen-acidovorax-avenae-subsp-avenae-strain-rs-2
#12
Md Mahidul Islam Masum, Yingzi Yang, Bin Li, Ogunyemi Solabomi Olaitan, Jie Chen, Yang Zhang, Yushi Fang, Wen Qiu, Yanli Wang, Guochang Sun
The Type VI secretion system (T6SS) is a class of macromolecular machine that is required for the virulence of gram-negative bacteria. However, it is still not clear what the role of T6SS in the virulence of rice bacterial brown stripe pathogen Acidovorax avenae subsp. avenae (Aaa) is. The aim of the current study was to investigate the contribution of T6SS in Aaa strain RS2 virulence using insertional deletion mutation and complementation approaches. This strain produced weak virulence but contains a complete T6SS gene cluster based on a genome-wide analysis...
September 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28910638/the-landscape-of-type-vi-secretion-across-human-gut-microbiomes-reveals-its-role-in-community-composition
#13
Adrian J Verster, Benjamin D Ross, Matthew C Radey, Yiqiao Bao, Andrew L Goodman, Joseph D Mougous, Elhanan Borenstein
Although gut microbiome composition is well defined, the mechanisms underlying community assembly remain poorly understood. Bacteroidales possess three genetic architectures (GA1-3) of the type VI secretion system (T6SS), an effector delivery pathway that mediates interbacterial competition. Here we define the distribution and role of GA1-3 in the human gut using metagenomic analysis. We find that adult microbiomes harbor limited effector and cognate immunity genes, suggesting selection for compatibility at the species (GA1 and GA2) and strain (GA3) levels...
September 13, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28889297/contribution-of-cyclic-di-gmp-in-the-control-of-type-iii-and-type-vi-secretion-in-pseudomonas-aeruginosa
#14
Ronan R McCarthy, Martina Valentini, Alain Filloux
Bacteria produce toxins to enhance their competitiveness in the colonization of an environment as well as during an infection. The delivery of toxins into target cells is mediated by several types of secretion systems, among them our focus is Type III and Type VI Secretion Systems (T3SS and T6SS, respectively). A thorough methodology is provided detailing how to identify if cyclic di-GMP signaling plays a role in the P. aeruginosa toxin delivery mediated by T3SS or T6SS. This includes in vitro preparation of the samples for Western blot analysis aiming at detecting possible c-di-GMP-dependent T3SS/T6SS switch, as well as in vivo analysis using the model organism Galleria mellonella to demonstrate the ecological and pathogenic consequence of the switch between these two secretion systems...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28848712/burkholderia-pseudomallei-evades-nramp1-slc11a1-and-nadph-oxidase-mediated-killing-in-macrophages-and-exhibits-nramp1-dependent-virulence-gene-expression
#15
Veerachat Muangsombut, Patoo Withatanung, Varintip Srinon, Narisara Chantratita, Mark P Stevens, Jenefer M Blackwell, Sunee Korbsrisate
Bacterial survival in macrophages can be affected by the natural resistance-associated macrophage protein 1 (Nramp1; also known as solute carrier family 11 member a1 or Slc11a1) which localizes to phagosome membranes and transports divalent cations, including iron. Little is known about the role of Nramp1 in Burkholderia infection, in particular whether this differs for pathogenic species like Burkholderia pseudomallei causing melioidosis or non-pathogenic species like Burkholderia thailandensis. Here we show that transfected macrophages stably expressing wild-type Nramp1 (Nramp1(+)) control the net replication of B...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28847494/isolated-pseudomonas-aeruginosa-strain-vih2-and-antagonistic-properties-against-ralstonia-solanacearum
#16
Xincheng Ge, Wei Wei, Gen Li, Mingming Sun, Huixin Li, Jun Wu, Feng Hu
The aim of this study was to isolates with antagonist activity against R. solanacearum. Thirty-two bacterial isolates were obtained from samples, and they were screened for potential antagonistic activity against R. Solanacearum. Using the agar spot method, ten out of the 21 tested bacteria showed antilisterial activity. VIH2 had the highest inhibitory effect on the growth of R. Solanacearum. Based on 16S rDNA and Biolog test analysis, the strain VIH2 was identified as Pseudomonas aeruginosa. Single-factor and Response Surface Methodology experiments were used to optimize the culture medium and conditions...
October 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28830939/a-transcriptional-regulatory-mechanism-finely-tunes-the-firing-of-type-vi-secretion-system-in-response-to-bacterial-enemies
#17
Martina Lazzaro, Mario F Feldman, Eleonora García Véscovi
The ability to detect and measure danger from an environmental signal is paramount for bacteria to respond accordingly, deploying strategies that halt or counteract potential cellular injury and maximize survival chances. Type VI secretion systems (T6SSs) are complex bacterial contractile nanomachines able to target toxic effectors into neighboring bacteria competing for the same colonization niche. Previous studies support the concept that either T6SSs are constitutively active or they fire effectors in response to various stimuli, such as high bacterial density, cell-cell contact, nutrient depletion, or components from dead sibling cells...
August 22, 2017: MBio
https://www.readbyqxmd.com/read/28818949/in-situ-architecture-function-and-evolution-of-a-contractile-injection-system
#18
Désirée Böck, João M Medeiros, Han-Fei Tsao, Thomas Penz, Gregor L Weiss, Karin Aistleitner, Matthias Horn, Martin Pilhofer
Contractile injection systems mediate bacterial cell-cell interactions by a bacteriophage tail-like structure. In contrast to extracellular systems, the type 6 secretion system (T6SS) is defined by intracellular localization and attachment to the cytoplasmic membrane. Here we used cryo-focused ion beam milling, electron cryotomography, and functional assays to study a T6SS in Amoebophilus asiaticus The in situ architecture revealed three modules, including a contractile sheath-tube, a baseplate, and an anchor...
August 18, 2017: Science
https://www.readbyqxmd.com/read/28818038/role-of-the-type-vi-secretion-systems-during-disease-interactions-of-erwinia-amylovora-with-its-plant-host
#19
Tim Kamber, Joël F Pothier, Cosima Pelludat, Fabio Rezzonico, Brion Duffy, Theo H M Smits
BACKGROUND: Type VI secretion systems (T6SS) are widespread among Gram-negative bacteria and have a potential role as essential virulence factors or to maintain symbiotic interactions. Three T6SS gene clusters were identified in the genome of E. amylovora CFBP 1430, of which T6SS-1 and T6SS-3 represent complete T6SS machineries, while T6SS-2 is reduced in its gene content. RESULTS: To assess the contribution of T6SSs to virulence and potential transcriptomic changes of E...
August 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28817192/tssa-the-cap-protein-of-the-type-vi-secretion-system-tail
#20
REVIEW
Abdelrahim Zoued, Eric Durand, Yoann G Santin, Laure Journet, Alain Roussel, Christian Cambillau, Eric Cascales
The Type VI secretion system (T6SS) is a multiprotein and mosaic apparatus that delivers protein effectors into prokaryotic or eukaryotic cells. Recent data on the enteroaggregative Escherichia coli (EAEC) T6SS have provided evidence that the TssA protein is a key component during T6SS biogenesis. The T6SS comprises a trans-envelope complex that docks the baseplate, a cytoplasmic complex that represents the assembly platform for the tail. The T6SS tail is structurally, evolutionarily and functionally similar to the contractile tails of bacteriophages...
August 17, 2017: BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology
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