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https://www.readbyqxmd.com/read/29224156/multiplexed-and-sensitive-dna-methylation-testing-using-methylation-sensitive-restriction-enzymes-msre-qpcr
#1
Gabriel Beikircher, Walter Pulverer, Manuela Hofner, Christa Noehammer, Andreas Weinhaeusel
DNA methylation is a chemically stable key-player in epigenetics. In the vertebrate genome the 5-methyl cytosine (5mC) has been found almost exclusively in the CpG dinucleotide context. CpG dinucleotides are enriched in CpG islands very frequently located within or close to gene promoters. Analyses of DNA methylation changes in human diagnostics have been conducted classically using methylation-sensitive restriction enzymes (MSRE). Since the discovery of bisulfite conversion-based sequencing and PCR assays, MSRE-based PCR assays have been less frequently used, although especially in the field of cancer epigenetics MSRE-based genome-wide discovery and targeted screening applications have been and are still performed successfully...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29223359/in-niemann-pick-c1-mouse-models-glial-only-expression-of-the-normal-gene-extends-survival-much-further-than-do-changes-in-genetic-background-or-treatment-with-hydroxypropyl-beta-cyclodextrin
#2
Craig A Marshall, Dawn E Watkins-Chow, Giampiero Palladino, Gail Deutsch, Keshav Chandran, William J Pavan, Robert P Erickson
The Npc1nmf164 allele of Npc1 provides a mouse model for Niemann-Pick disease type C1 (NPC1), a genetic disease known to have a widely variable phenotype. The transfer of the Npc1nmf164 mutation from the C57BL/6J inbred strain to the BALB/cJ inbred strain increased the mean lifespan from 117.8days to 153.1days, confirming that the severity of the NPC1 phenotype is strongly influenced by genetic background. The transfer of another Npc1 allele, Npc1nih, to this background also extended survival of the homozygotes indicating that the modifying effect of BALB/cJ is not limited to a single allele of Npc1...
December 6, 2017: Gene
https://www.readbyqxmd.com/read/29219458/detection-of-microwave-transitions-between-ortho-and-para-states-in-a-free-isolated-molecule
#3
Hideto Kanamori, Zeinab T Dehghani, Asao Mizoguchi, Yasuki Endo
Microwave transitions between the ortho and para states of the S_{2}Cl_{2} molecule in a free isolated condition are observed for the first time. In the theoretical treatment, we derive eigenfunctions of an effective Hamiltonian including the ortho-para interaction to calculate the intensities and frequencies of forbidden ortho-para transitions in the cm-wave region and pick up some promising candidates for the spectroscopic detection. In the experiment, transitions of the S_{2}Cl_{2} molecule under a supersonic jet condition are observed with a Fourier transform microwave spectrometer...
October 27, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/29218847/potential-exposure-to-australian-bat-lyssavirus-is-unlikely-to-prevent-future-bat-handling-among-adults-in-south-east-queensland
#4
M K Young, S Banu, B J McCall, S Vlack, H Carroll, S Bennett, R Davison, D Francis
Despite ongoing public health messages about the risks associated with bat contact, the number of potential exposures to Australian bat lyssavirus (ABLV) due to intentional handling by members of the general public in Queensland has remained high. We sought to better understand the reasons for intentional handling among these members of the public who reported their potential exposure to inform future public health messages. We interviewed adults who resided in a defined geographic area in South East Queensland and notified potential exposure to ABLV due to intentional handling of bats by telephone between 1 January 2012 and 31 December 2013...
December 8, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/29218843/potential-of-saprophage-diptera-to-acquire-culturable-livestock-associated-antibiotic-resistant-bacteria
#5
S M Russell, V A Chittick, D T Sangweme, E C Lampert
The increasing prevalence of antibiotic resistance among bacteria is one of the most intractable challenges in 21st-century public health. Dipterans that associate with livestock, livestock waste products and cadavers have the potential to acquire livestock-associated antibiotic-resistant bacteria (LA-ARB) and transmit them to humans. In this study, piglet cadavers were used to attract saprophage dipterans from the environment and those dipterans were sampled for the presence of LA-ARB. In the first trial, culturable microbes resistant to both aminoglycoside and β-lactam antibiotics were found in all cadavers and masses of dipteran larvae, and in three-quarters of adult dipterans...
December 8, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/29218753/neuronal-responses-to-the-scratching-and-caressing-of-one-s-own-skin-in-patients-with-skin-picking-disorder
#6
Anne Schienle, Sonja Übel, Albert Wabnegger
Skin-picking disorder (SPD) is a common mental disorder. The predominant symptom involves the repeated scratching and picking of one's own skin. This behavior causes severe tissue damage (sores, scars, and infections), often leading to disfigurement. Besides physical injury, SPD is associated with clinically significant distress and impairment in important areas of functioning. The neurobiological mechanisms of SPD are still poorly understood. In this study, 30 SPD patients and 31 control participants (35 women, 26 men) with a mean age of 34 years were instructed to either scratch or gently stroke a small skin area on their arms during functional magnetic resonance imaging...
December 7, 2017: Human Brain Mapping
https://www.readbyqxmd.com/read/29217709/bradford-c-berk-picking-good-questions
#7
Sandra J Ackerman
No abstract text is available yet for this article.
December 8, 2017: Circulation Research
https://www.readbyqxmd.com/read/29217520/alex-azar-critics-cry-revolving-door-as-trump-picks-pharma-executive-for-top-us-health-job
#8
Liz Seegert
No abstract text is available yet for this article.
December 7, 2017: BMJ: British Medical Journal
https://www.readbyqxmd.com/read/29216772/proteomic-cinderella-customized-analysis-of-bulky-ms-ms-data-in-one-night
#9
Olga Kiseleva, Ekaterina Poverennaya, Alexander Shargunov, Andrey Lisitsa
Proteomic challenges, stirred up by the advent of high-throughput technologies, produce large amount of MS data. Nowadays, the routine manual search does not satisfy the "speed" of modern science any longer. In our work, the necessity of single-thread analysis of bulky data emerged during interpretation of HepG2 proteome profiling results for proteoforms searching. We compared the contribution of each of the eight search engines (X!Tandem, MS-GF[Formula: see text], MS Amanda, MyriMatch, Comet, Tide, Andromeda, and OMSSA) integrated in an open-source graphical user interface SearchGUI ( http://searchgui...
November 13, 2017: Journal of Bioinformatics and Computational Biology
https://www.readbyqxmd.com/read/29216122/delay-line-coherent-pulse-stacking
#10
Henrik Tünnermann, Akira Shirakawa
Ultrafast fiber amplifiers are usually limited in terms of pulse energy and peak power due to fiber damage and nonlinearities. On the other hand, fiber amplifiers are very energy efficient. To take advantage of this efficiency, but still scale the pulse energy, coherent combining and pulse stacking have become the method of choice. Here we show how to directly and symmetrically stack up the pulses from an oscillator chain, using delay lines and phase preshaping of a 48 MHz, 1.5 μm master oscillator. This method avoids the need for high-power low-loss dumpers as in passive stack and dump cavities and, compared to divided pulse amplification (DPA), it eliminates the need for splitting pulses and pulse picking and, thus, optimally utilizes the available seed power...
December 1, 2017: Optics Letters
https://www.readbyqxmd.com/read/29215752/familial-tauopathy-with-p364s-mapt-mutation-clinical-course-neuropathology-and-ultrastructure-of-neuronal-tau-inclusions
#11
Peter Štrafela, Jerica Pleško, Jožef Magdič, Blaž Koritnik, Andrej Zupan, Damjan Glavač, Mara Bresjanac, Mara Popović
AIMS: This report presents the clinical course, neuropathology and ultrastructure of neuronal tau inclusions of four Slovene relatives with P364S MAPT mutation. METHODS: The clinical history of three out of four P364S MAPT mutation carriers was taken. After formalin fixation, thorough sampling of the central nervous system was followed by paraffin embedding, H&E, Gallyas, Bielschowsky, and immunostaining with AT8, anti-3R, anti-4R tau, anti-Amyloid-β, anti-TDP43, and anti-alpha-synuclein antibodies...
December 7, 2017: Neuropathology and Applied Neurobiology
https://www.readbyqxmd.com/read/29214754/utility-of-conventional-culture-and-maldi-tof-ms-for-identification-of-microbial-communities-in-bronchoalveolar-lavage-fluid-in-comparison-with-the-gs-junior-next-generation-sequencing-system
#12
Ji Yeon Sung, Younjee Hwang, Mi Hwa Shin, Moo Suk Park, Sang Hoon Lee, Dongeun Yong, Kyungwon Lee
BACKGROUND: Diverse microbiota exist in the lower respiratory tract. Although next generation sequencing (NGS) is the most widely used microbiome analysis technique, it is difficult to implement NGS in clinical microbiology laboratories. Therefore, we evaluated the performance of conventional culture methods together with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in identifying microbiota in bronchoalveolar lavage (BAL) fluid. METHODS: BAL fluid samples (n=27) were obtained from patients undergoing diagnostic bronchoscopy for lung mass evaluation...
March 2018: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/29214445/auxin-enhances-grafting-success-in-carya-cathayensis-chinese-hickory
#13
R M Saravana Kumar, Liu Xiao Gao, Hu Wei Yuan, Dong Bin Xu, Zhao Liang, Shen Chen Tao, Wen Bin Guo, Dao Liang Yan, Bing Song Zheng, Johan Edqvist
Application of auxin to root stock and scion increases the success rate of grafting in Chinese hickory. The nuts of the Chinese hickory (Carya cathayensis) tree are considered both delicious and healthy. The popularity and high demand result is that the hickory nuts are of very high economical value for horticulture. This is particularly true for the Zhejiang province in eastern China where this tree is widely cultivated. However, there are several difficulties surrounding the hickory cultivation, such as for example long vegetative growth, tall trees, labour-intensive nut picking, and slow variety improvements...
December 6, 2017: Planta
https://www.readbyqxmd.com/read/29213187/the-role-of-the-whitefly-bemisia-tabaci-gennadius-and-farmer-practices-in-the-spread-of-cassava-brown-streak-ipomoviruses
#14
Midatharahally N Maruthi, Simon C Jeremiah, Ibrahim U Mohammed, James P Legg
Cassava brown streak disease (CBSD) is arguably the most dangerous current threat to cassava, which is Africa's most important food security crop. CBSD is caused by two RNA viruses: Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). The roles of the whitefly Bemisia tabaci (Gennadius) and farmer practices in the spread of CBSD were investigated in a set of field and laboratory experiments. The virus was acquired and transmitted by B. tabaci within a short time (5-10 min each for virus acquisition and inoculation), and was retained for up to 48 hr...
December 2017: Journal of Phytopathology (1986)
https://www.readbyqxmd.com/read/29212933/characterization-of-an-influenza-virus-pseudotyped-with-ebolavirus-glycoprotein
#15
Julie Xiao, Pramila Rijal, Lisa Schimanski, Arun Kumar Tharkeshwar, Edward Wright, Wim Annaert, Alain Townsend
We have produced a new Ebola virus pseudotype: E-S-FLU, which can be handled in biosafety level-1/2 containment for laboratory analysis. E-S-FLU is a single cycle influenza virus coated with Ebolavirus glycoprotein, and it encodes enhanced green fluorescence protein as a reporter that replaces the influenza haemagglutinin. MDCK-SIAT1 cells were transduced to express Ebolavirus glycoprotein as a stable transmembrane protein for E-S-FLU production. Infection of cells by E-S-FLU was dependent on Niemann-Pick C1 protein, which is the well-characterized receptor for Ebola virus entry at the late endosome/lysosome membrane...
December 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29211889/reduced-live-birth-rates-after-ivf-icsi-in-women-with-previous-unilateral-oophorectomy-results-of-a-multicentre-cohort-study
#16
Tekla Lind, Jan Holte, Jan I Olofsson, Nermin Hadziosmanovic, Johannes Gudmundsson, Elizabeth Nedstrand, Mikael Lood, Lars Berglund, Kenny Rodriguez-Wallberg
STUDY QUESTION: Is there a reduced live-birth rate (LBR) after IVF/ICSI treatment in women with a previous unilateral oophorectomy (UO)? SUMMARY ANSWER: A significantly reduced LBR after IVF/ICSI was found in women with previous UO when compared with women with intact ovaries in this large multicentre cohort, both crudely and after adjustment for age, BMI, fertility centre and calendar period and regardless of whether the analysis was based on transfer of embryos in the fresh cycle only or on cumulative results including transfers using frozen-thawed embryos...
December 4, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29210980/superconducting-quantum-interferometers-for-nondestructive-evaluation
#17
REVIEW
M I Faley, E A Kostyurina, K V Kalashnikov, Yu V Maslennikov, V P Koshelets, R E Dunin-Borkowski
We review stationary and mobile systems that are used for the nondestructive evaluation of room temperature objects and are based on superconducting quantum interference devices (SQUIDs). The systems are optimized for samples whose dimensions are between 10 micrometers and several meters. Stray magnetic fields from small samples (10 µm-10 cm) are studied using a SQUID microscope equipped with a magnetic flux antenna, which is fed through the walls of liquid nitrogen cryostat and a hole in the SQUID's pick-up loop and returned sidewards from the SQUID back to the sample...
December 6, 2017: Sensors
https://www.readbyqxmd.com/read/29208984/a-nonlinear-approach-to-identify-pathological-change-of-thyroid-nodules-based-on-statistical-analysis-of-ultrasound-rf-signals
#18
Huan Xu, Chunrui Liu, Ping Yang, Juan Tu, Bin Yang, Dong Zhang
In order to reassure the majority of patients with benign nodules from unnecessary needle biopsy, there is an increasing clinical requirement to identify benign and malignant thyroid nodules during ultrasound diagnosis. A nonlinear approach based on statistical analysis of ultrasound radio-frequency (RF) signals was developed for differential diagnosing the thyroid nodules to improve the diagnostic accuracy. Data from 44 patients with solitary thyroid nodules were collected, following with the ultrasound-guided fine needle aspiration (FNA) as the ground truth...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29208824/terminal-chop-new-technique-for-full-thickness-nuclear-segmentation-in-mature-hard-cataract
#19
Rajendra Prasad, Anurag Badhani, Geetika Badhani Dogra
We describe an efficient technique, "Terminal Chop," for full thickness nuclear segmentation in mature hard cataracts. Terminal chop utilizes the principle of secondary rock breakage system with stress concentration to break these hard nuclei. In this technique consonant to drag picks, a specially designed chopper "Terminator" is used to initiate a unique dispersive mechanical force to create a full thickness nuclear crack (crack formation) at the weakest soft and thin equator, which automatically traverses through the center and to the equator on the other side...
December 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29208808/intraocular-lens-calculations-in-atypical-eyes
#20
REVIEW
Aazim A Siddiqui, Uday Devgan
Cataract surgery is the most performed surgical procedure in the field of ophthalmology. The process of intraocular lens (IOL) calculations is a critical step to achieving successful outcomes. Many IOL formulae exist to guide surgeons through the difficult process of picking the most appropriate lens to achieve a certain target refraction. However, these formulae reach within 0.50 diopters of the target refraction only 75% of the time, leaving 25% of the eyes with a significant refractive surprise. A literature review was performed to investigate all the relevant published material on the history, progress, and recent advancements of IOL calculations...
December 2017: Indian Journal of Ophthalmology
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