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https://www.readbyqxmd.com/read/28228962/conforming-with-current-regulation-in-turkey-regarding-the-freezing-of-oocytes-a-case-report-of-the-first-pregnancy-in-turkey-achieved-through-oocyte-vitrification
#1
Semra Kahraman, Kahraman Ulker, Caroline Pirkevi Çetinkaya, Yesim Kumtepe Colakoglu, Hakan K Yelke, Cihangir Yilanlioglu
OBJECTIVES: To present the first pregnancy achieved in Turkey with frozen-warmed oocytes in a case with previous nine unsuccessful assisted reproductive technology (ART) attempts. METHODS: The clinical follow-up of a 33-year-old female applying to our ART centre after a long and complicated history of infertility is described. RESULTS: In April 2013, the woman attempted our centre for her 10th ART trial. She informed us on oocyte pick-up (OPU) day that her husband had been hospitalized following a car crush in Albania and was unable to travel to our clinic to give a sperm sample...
2017: SAGE open medical case reports
https://www.readbyqxmd.com/read/28228741/perception-and-modeling-of-affective-qualities-of-musical-instrument-sounds-across-pitch-registers
#2
Stephen McAdams, Chelsea Douglas, Naresh N Vempala
Composers often pick specific instruments to convey a given emotional tone in their music, partly due to their expressive possibilities, but also due to their timbres in specific registers and at given dynamic markings. Of interest to both music psychology and music informatics from a computational point of view is the relation between the acoustic properties that give rise to the timbre at a given pitch and the perceived emotional quality of the tone. Musician and nonmusician listeners were presented with 137 tones produced at a fixed dynamic marking (forte) playing tones at pitch class D# across each instrument's entire pitch range and with different playing techniques for standard orchestral instruments drawn from the brass, woodwind, string, and pitched percussion families...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28228113/validation-of-an-ngs-mutation-detection-panel-for-melanoma
#3
Anne Reiman, Hugh Kikuchi, Daniela Scocchia, Peter Smith, Yee Wah Tsang, David Snead, Ian A Cree
BACKGROUND: Knowledge of the genotype of melanoma is important to guide patient management. Identification of mutations in BRAF and c-KIT lead directly to targeted treatment, but it is also helpful to know if there are driver oncogene mutations in NRAS, GNAQ or GNA11 as these patients may benefit from alternative strategies such as immunotherapy. METHODS: While polymerase chain reaction (PCR) methods are often used to detect BRAF mutations, next generation sequencing (NGS) is able to determine all of the necessary information on several genes at once, with potential advantages in turnaround time...
February 22, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28228103/disease-manifestations-and-burden-of-illness-in-patients-with-acid-sphingomyelinase-deficiency-asmd
#4
REVIEW
Margaret M McGovern, Ruzan Avetisyan, Bernd-Jan Sanson, Olivier Lidove
Acid sphingomyelinase deficiency (ASMD), a rare lysosomal storage disease, is an autosomal recessive genetic disorder caused by different SMPD1 mutations. Historically, ASMD has been classified as Niemann-Pick disease (NPD) types A (NPD A) and B (NPD B). NPD A is associated with a uniformly devastating disease course, with rapidly progressing psychomotor degeneration, leading to death typically by the age of 3 years, most often from respiratory failure. In contrast, the clinical phenotype and life expectancy of patients with NPD B may vary widely...
February 23, 2017: Orphanet Journal of Rare Diseases
https://www.readbyqxmd.com/read/28227798/upper-limb-kinematics-of-adults-with-cerebral-palsy-on-bilateral-functional-tasks
#5
Carina Lott, Michelle J Johnson, Carina Lott, Michelle J Johnson, Carina Lott, Michelle J Johnson
Adults with cerebral palsy (CP) often show upper limb impairments which impact their ability to execute activities of daily living (ADLs). Three adults with CP and five healthy adults performed three types of bilateral and unilateral ADLs: drink, pour, and pick and place tasks. An affordable bilateral assessment system (BiAS) was used to measure wrist kinematic trajectories. Four metrics, total completion time, maximum velocity, smoothness, and phase difference, were used to evaluate each functional task. Overall, adults with CP took a longer time than the healthy subjects to complete all unilateral functional tasks with their non-dominant hand...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227496/movement-imagery-classification-in-emotiv-cap-based-system-by-nai%C3%AC-ve-bayes
#6
Vinicius N Stock, Alexandre Balbinot, Vinicius N Stock, Alexandre Balbinot, Vinicius N Stock, Alexandre Balbinot
Brain-computer interfaces (BCI) provide means of communications and control, in assistive technology, which do not require motor activity from the user. The goal of this study is to promote classification of two types of imaginary movements, left and right hands, in an EMOTIV cap based system, using the Naïve Bayes classifier. A preliminary analysis with respect to results obtained by other experiments in this field is also conducted. Processing of the electroencephalography (EEG) signals is done applying Common Spatial Pattern filters...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28224901/an-ssdna-library-immobilized-selex-technique-for-selection-of-an-aptamer-against-ractopamine
#7
Nuo Duan, Wenhui Gong, Shijia Wu, Zhouping Wang
An improved SELEX technique was developed for selecting aptamers against ractopamine (RAC) by immobilizing ssDNA library on the magnetic beads. After sixteen selection rounds, a highly enriched ssDNA pool was sequenced and nine families were grouped according to their homology and secondary structures analysis. One representative aptamer candidate from each family was picked out for binding affinity identification by graphene oxide (GO) adsorption platform. The aptamer RAC-6 was demonstrated as the optimal aptamer with high specificity and dissociation constant (Kd) value of 54...
April 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28223973/a-perspective-study-of-koumiss-microbiome-by-metagenomics-analysis-based-on-single-cell-amplification-technique
#8
Guoqiang Yao, Jie Yu, Qiangchuan Hou, Wenyan Hui, Wenjun Liu, Lai-Yu Kwok, Bilige Menghe, Tiansong Sun, Heping Zhang, Wenyi Zhang
Koumiss is a traditional fermented dairy product and a good source for isolating novel bacteria with biotechnology potential. In the present study, we applied the single-cell amplification technique in the metagenomics analysis of koumiss. This approach aimed at detecting the low-abundant bacteria in the koumiss. Briefly, each sample was first serially diluted until reaching the level of approximately 100 cells. Then, three diluted bacterial suspensions were randomly picked for further study. By analyzing 30 diluted koumiss suspensions, a total of 24 bacterial species were identified...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28222799/assessment-of-plasma-chitotriosidase-activity-ccl18-parc-concentration-and-np-c-suspicion-index-in-the-diagnosis-of-niemann-pick-disease-type-c-a-prospective-observational-study
#9
Isabel De Castro-Orós, Pilar Irún, Jorge Javier Cebolla, Victor Rodriguez-Sureda, Miguel Mallén, María Jesús Pueyo, Pilar Mozas, Carmen Dominguez, Miguel Pocoví
BACKGROUND: Niemann-Pick disease type C (NP-C) is a rare, autosomal recessive neurodegenerative disease caused by mutations in either the NPC1 or NPC2 genes. The diagnosis of NP-C remains challenging due to the non-specific, heterogeneous nature of signs/symptoms. This study assessed the utility of plasma chitotriosidase (ChT) and Chemokine (C-C motif) ligand 18 (CCL18)/pulmonary and activation-regulated chemokine (PARC) in conjunction with the NP-C suspicion index (NP-C SI) for guiding confirmatory laboratory testing in patients with suspected NP-C...
February 21, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28222592/confirmation-of-1-phenylethane-1-thiol-as-the-character-impact-aroma-compound-in-curry-leaves-and-its-behavior-during-tissue-disruption-drying-and-frying
#10
Martin Steinhaus
The most odor-active compounds previously identified by application of an aroma extract dilution analysis were quantitated in freshly picked curry leaves, either by stable isotope dilution assays in combination with GC-GC-MS or by GC-FID after simultaneous extraction/fractionation. Odor activity values (OAVs) were calculated as ratios of concentrations to odor threshold values. The topmost OAVs were obtained for (3Z)-hex-3-enal (grassy; OAV 180000), (1S)-1-phenylethane-1-thiol (sulfury, burnt; OAV 150000), (1R)-1-phenylethane-1-thiol (sulfury, burnt; OAV 120000), (3R)-linalool (citrusy; OAV 58000), and myrcene (geranium leaf-like; OAV 23000)...
February 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28222331/the-moro-reaction-more-than-a-reflex-a-ritualized-behavior-of-nonverbal-communication
#11
Pierre V Rousseau, Florence Matton, Renaud Lecuyer, Willy Lahaye
OBJECTIVE: To propose a phylogenetic significance to the Moro reflex which remains unexplained since its publication in 1918 because both hands are free at the end of the gesture. METHOD: Among the 75 videos of healthy term newborns we have filmed in a research project on antenatal education to parenthood, we describe a sequence that clearly showed the successive movements of the Moro reflex and we report the occurrence of this reflex in the videos that were recorded from Time 0 of birth defined as the moment that lies between the birth of the thorax and the pelvis of the infant...
February 18, 2017: Infant Behavior & Development
https://www.readbyqxmd.com/read/28215678/the-impact-of-ovarian-stimulation-protocol-on-oocyte-quality-subsequent-in%C3%A2-vitro-embryo-development-and-pregnancy-after-transfer-to-recipients-in-eld-s-deer-rucervus-eldii-thamin
#12
Ampika Thongphakdee, Debra K Berg, Theerawat Tharasanit, Nikorn Thongtip, Wanlaya Tipkantha, Chainarong Punkong, Daraka Tongthainan, Sakhon Noimoon, Umaporn Maikeaw, Nudthakamol Kajornklin, Boripat Siriaroonrat, Pierre Comizzoli, Sumate Kamolnorranath
Propagating genetically valuable individuals through oocyte collection, in vitro fertilization (IVF) and embryo transfer is critical to maintain sustainable populations of the endangered Eld's deer. The objectives of this study were to assess the impact of exogenous FSH injections on (1) the number and in vitro competence of oocytes collected and (2) the developmental potential of resulting IVF embryos after transfer into recipients during the breeding season (February-April). In a pilot experiment, estrus synchronization was conducted in three surplus females (using intravaginal progesterone-releasing devices, CIDRG for 14 days and injections of buserelin (a GnRH agonist)...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28214330/automated-microfluidic-sorting-of-mammalian-cells-labeled-with-magnetic-microparticles-for-those-that-efficiently-express-and-secrete-a-protein-of-interest
#13
Xuan Droz, Niamh Harraghy, Etienne Lançon, Valérie Le Fourn, David Calabrese, Thierry Colombet, Pascal Liechti, Amar Rida, Pierre-Alain Girod, Nicolas Mermod
We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after capture. Using clinically compatible materials and procedures, we show that this approach is able to discriminate between cells that truly secrete high amounts of a protein from those that just display it at high levels on their surface without properly releasing it...
February 18, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28214032/picking-on-fibroblast-growth-factor-receptors-as-bladder-cancer-therapeutic-targets
#14
EDITORIAL
Miriam Marqués, Francisco X Real
No abstract text is available yet for this article.
February 14, 2017: European Urology
https://www.readbyqxmd.com/read/28213793/automation-of-peak-tracking-analysis-of-stepwise-perturbed-nmr-spectra
#15
Tommaso Banelli, Marco Vuano, Federico Fogolari, Andrea Fusiello, Gennaro Esposito, Alessandra Corazza
We describe a new algorithmic approach able to automatically pick and track the NMR resonances of a large number of 2D NMR spectra acquired during a stepwise variation of a physical parameter. The method has been named Trace in Track (TINT), referring to the idea that a gaussian decomposition traces peaks within the tracks recognised through 3D mathematical morphology. It is capable of determining the evolution of the chemical shifts, intensity and linewidths of each tracked peak.The performances obtained in term of track reconstruction and correct assignment on realistic synthetic spectra were high above 90% when a noise level similar to that of experimental data were considered...
February 17, 2017: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/28213572/impact-of-expanding-the-prehospital-stroke-bypass-time-window-in-a-large-geographic-region
#16
Ian G Stiell, Catherine M Clement, Kristy Campbell, Mukul Sharma, Doug Socha, Marco L A Sivilotti, Albert Jin, Jeffrey J Perry, Jim Lumsden, Cally Martin, Mark Froats, Richard Dionne, John Trickett
BACKGROUND AND PURPOSE: The Ontario Acute Stroke Medical Redirect Paramedic Protocol (ASMRPP) was revised to allow paramedics to bypass to designated stroke centers if total transport time would be <2 hours and total time from symptom onset <3.5 hours. We sought to evaluate the impact and safety of implementing the Revised ASMRPP. METHODS: We conducted a 12-month implementation study involving prehospital patients presenting with possible stroke symptoms. A total of 1317 basic and advanced life support paramedics, of 9 land services in 10 rural counties and 5 cities, used the Revised ASMRPP to take appropriate patients directly to 6 designated stroke centers...
February 17, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/28213211/application-of-2d-hplc-coupled-with-principal-component-analysis-to-study-an-industrial-opiate-processing-stream
#17
Paul G Stevenson, Niki K Burns, Stuart D Purcell, Paul S Francis, Neil W Barnett, Fiona Fry, Xavier A Conlan
Principal component analysis (PCA) loading plots were used to elucidate key differences between two-dimensional high performance liquid chromatography (2D-HPLC) fingerprint data from samples collected from stages along the Papaver somniferum industrial process chemistry workflow. Data reduction was completed using a 2D-HPLC peak picking approach as a precursor to chemometric analysis. Using comparisons of the final stages of product extraction as an example, PCA analysis of characteristic 2D-HPLC fingerprints accounted for 84...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28210186/bilateral-retinitis-pigmentosa-with-unilateral-choroidal-nevus-a-hitherto-unreported-association
#18
Rupak Roy, Kumar Saurabh, Debmalya Das, Preeti Sharma, Avirupa Ghose, Dhileesh P Chandrasekharan
Retinitis Pigmentosa (RP) has been reported to be associated with retinal hemangioma, retinal angiomatous proliferation, polypoidal choroidal vasculopathy and vasoproliferative tumours. However there is no previous report of choroidal nevus in an eye with RP. We describe such a case in which the eye with RP had a choroidal nevus at the macula. The coexistence of these two entities in the same eye emphasizes the need of careful examination of patients with RP. The presence of choroidal nevus warrants additional investigations such as B scan ultrasonography and a close follow-up to pick up changes in the nevus size and morphology...
July 2016: Saudi Journal of Ophthalmology: Official Journal of the Saudi Ophthalmological Society
https://www.readbyqxmd.com/read/28209532/stability-of-problematic-eating-behaviors-and-weight-loss-trajectories-after-bariatric-surgery-a-longitudinal-observational-study
#19
Eva M Conceição, James E Mitchell, Ana Pinto-Bastos, Filipa Arrojado, Isabel Brandão, Paulo P P Machado
BACKGROUND: The literature is rather mixed regarding the stability and the role of pre- and postoperative problematic eating behaviors (PEBs) on weight outcomes after bariatric surgery. OBJECTIVES: To investigate the stability of loss of control (LOC) eating and picking and/or nibbling from pre- to postoperative assessments, and to investigate whether pre- and postoperative PEBs are predictors of different weight loss trajectories. SETTING: Central Hospital, University, Portugal...
December 20, 2016: Surgery for Obesity and related Diseases: Official Journal of the American Society for Bariatric Surgery
https://www.readbyqxmd.com/read/28209214/effects-of-concentrate-type-and-chromium-propionate-on-insulin-sensitivity-productive-and-reproductive-parameters-of-lactating-dairy-cows-consuming-excessive-energy
#20
T Leiva, R F Cooke, A P Brandão, U Pardelli, R O Rodrigues, F N Corrá, J L M Vasconcelos
This experiment compared insulin sensitivity parameters, milk production and reproductive outcomes in lactating dairy cows consuming excessive energy, and receiving in a 2×2 factorial arrangement design: (1) concentrate based on ground corn (CRN; n=13) or citrus pulp (PLP; n=13), and (2) supplemented (n=14) or not (n=12) with 2.5 g/day of chromium (Cr)-propionate. During the experiment (day 0 to 182), 26 multiparous, non-pregnant, lactating Gir×Holstein cows (initial days in milk=80±2) were offered corn silage for ad libitum consumption, and individually received concentrate formulated to allow diets to provide 160% of their daily requirements of net energy for lactation...
March 2017: Animal: An International Journal of Animal Bioscience
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