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https://www.readbyqxmd.com/read/28800588/non-lethal-genotyping-of-tribolium-castaneum-adults-using-genomic-dna-extracted-from-wing-tissue
#1
Frederic Strobl, J Alexander Ross, Ernst H K Stelzer
The red flour beetle Tribolium castaneum has become the second most important insect model organism and is frequently used in developmental biology, genetics and pest-associated research. Consequently, the methodological arsenal increases continuously, but many routinely applied techniques for Drosophila melanogaster and other insect species are still unavailable. For example, a protocol for non-lethal genotyping has not yet been adapted but is particularly useful when individuals with known genotypes are required for downstream experiments...
2017: PloS One
https://www.readbyqxmd.com/read/28800286/clinical-pet-imaging-in-prostate-cancer
#2
Kathryn L Wallitt, Sairah R Khan, Suraiya Dubash, Henry H Tam, Sameer Khan, Tara D Barwick
Prostate cancer is the second most common cancer in men worldwide, with a wide spectrum of biologic behavior ranging from indolent low-risk disease to highly aggressive castration-resistant prostate cancer. Conventional imaging with computed tomography, magnetic resonance imaging, and bone scintigraphy is limited for the detection of nodal disease and distant bone metastases. In addition, advances in the available therapeutic options, both localized and systemic, drive the requirement for precise diagnostic and prognostic tools to refine the individual therapeutic approach at various times in the management of patients with prostate cancer...
August 11, 2017: Radiographics: a Review Publication of the Radiological Society of North America, Inc
https://www.readbyqxmd.com/read/28799992/guided-growth-novel-applications-in-the-hip-knee-and-ankle
#3
Maryse Bouchard
Hemiepiphyseodesis for angular deformities in the growing child is effective and avoids the complications of osteotomies and gradual correction with external fixation. This article reviews applications of screw hemiepiphyseodesis for coronal and sagittal plane deformities in the lower extremity. Indications, technical pearls, and pitfalls are discussed.
September 2017: Journal of Pediatric Orthopedics
https://www.readbyqxmd.com/read/28798934/comparative-phenotype-and-genome-analysis-of-cellvibrio-sp-pr1-a-xylanolytic-and-agarolytic-bacterium-from-the-pearl-river
#4
Zhangzhang Xie, Weitie Lin, Jianfei Luo
Cellvibrio sp. PR1 is a xylanolytic and agarolytic bacterium isolated from the Pearl River. Strain PR1 is closely related to Cellvibrio fibrivorans and C. ostraviensis (identity > 98%). The xylanase and agarase contents of strain PR1 reach up to 15.4 and 25.9 U/mL, respectively. The major cellular fatty acids consisted of C16:0 (36.7%), C18:0 (8.8%), C20:0 (6.8%), C15:0 iso 2-OH or/and C16:1ω7c (17.4%), and C18:1ω7c or/and C18:1ω6c (6.7%). A total of 251 CAZyme modules (63 CBMs, 20 CEs, 128 GHs, 38 GTs, and 2 PLs) were identified from 3,730 predicted proteins...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28798755/pearl-millet-genetic-traits-shape-rhizobacterial-diversity-and-modulate-rhizosphere-aggregation
#5
Papa M S Ndour, Mariama Gueye, Mohamed Barakat, Philippe Ortet, Marie Bertrand-Huleux, Anne-Laure Pablo, Damien Dezette, Lydie Chapuis-Lardy, Komi Assigbetsé, Ndjido Ardo Kane, Yves Vigouroux, Wafa Achouak, Ibrahima Ndoye, Thierry Heulin, Laurent Cournac
Root exudation contributes to soil carbon allocation and also to microbial C and energy supply, which subsequently impacts soil aggregation around roots. Biologically-driven soil structural formation is an important driver of soil fertility. Plant genetic determinants of exudation and more generally of factors promoting rhizosphere soil aggregation are largely unknown. Here, we characterized rhizosphere aggregation in a panel of 86 pearl millet inbred lines using a ratio of root-adhering soil dry mass per root tissue dry mass (RAS/RT)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28796757/summary-of-notifiable-infectious-diseases-and-conditions-united-states-2015
#6
Deborah A Adams, Kimberly R Thomas, Ruth Ann Jajosky, Loretta Foster, Gitangali Baroi, Pearl Sharp, Diana H Onweh, Alan W Schley, Willie J Anderson
The Summary of Notifiable Infectious Diseases and Conditions - United States, 2015 (hereafter referred to as the summary) contains the official statistics, in tabular and graphical form, for the reported occurrence of nationally notifiable infectious diseases and conditions in the United States for 2015. Unless otherwise noted, data are final totals for 2015 reported as of June 30, 2016. These statistics are collected and compiled from reports sent by U.S. state and territories, New York City, and District of Columbia health departments to the National Notifiable Diseases Surveillance System (NNDSS), which is operated by CDC in collaboration with the Council of State and Territorial Epidemiologists (CSTE)...
August 11, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/28794661/focused-assessment-with-sonography-for-trauma-current-perspectives
#7
REVIEW
Sorravit Savatmongkorngul, Sirote Wongwaisayawan, Rathachai Kaewlai
Focused assessment with sonography for trauma (FAST) is a part of resuscitation of trauma patients recommended by international panel consensus. The purpose of FAST is to identify free fluid, which necessarily means blood in acute trauma patients. In this article, the authors focused on various aspects of FAST in the emergency department, prehospital care, pediatric setting, training and general pearls/pitfalls. Detailed techniques and interpretation of FAST are beyond the scope of this article.
2017: Open Access Emergency Medicine: OAEM
https://www.readbyqxmd.com/read/28794040/pathobiology-of-clade-2-3-4-4-h5nx-high-pathogenicity-avian-influenza-virus-infections-in-minor-gallinaceous-poultry-supports-early-backyard-flock-introductions-in-western-u-s-2014-2015
#8
Kateri Bertran, Dong-Hun Lee, Mary J Pantin-Jackwood, Erica Spackman, Charles Balzli, David L Suarez, David E Swayne
In 2014-2015, the U.S. experienced an unprecedented outbreak of Eurasian clade 2.3.4.4 H5 highly pathogenic avian influenza (HPAI) virus. Initial cases affected mainly wild birds and mixed backyard poultry species, while later outbreaks affected mostly commercial chickens and turkeys. The pathogenesis, transmission, and intra-host evolutionary dynamics of initial Eurasian H5N8 and reassortant H5N2 clade 2.3.4.4 HPAI viruses in the U.S. were investigated in minor gallinaceous poultry species (i.e. species for which the U...
August 9, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28792290/pearls-and-pitfalls-for-prescribing-opioids-in-a-climate-of-increased-provider-scrutiny
#9
Joanna Gedzior, Arlen Kwong
In August 2016, U.S. Surgeon General Vivek Murthy, MD, MBA, issued a letter to healthcare providers requesting aid in addressing "an urgent health crisis facing America: the opioid epidemic." In this article, we address some of the more poignant challenges that surface in treating patients with opiate addiction. We provide an outline of recommendations from the leading medical organizations to educate primary care providers on how to navigate patients to decreased or discontinued medication loads.
March 2017: International Journal of Psychiatry in Medicine
https://www.readbyqxmd.com/read/28782039/control-of-nacre-biomineralization-by-pif80-in-pearl-oyster
#10
So Yeong Bahn, Byung Hoon Jo, Yoo Seong Choi, Hyung Joon Cha
Molluscan nacre is a fascinating biomineral consisting of a highly organized calcium carbonate composite that provides unique fracture toughness and an iridescent color. Organisms elaborately control biomineralization using organic macromolecules. We propose the involvement of the matrix protein Pif80 from the pearl oyster Pinctada fucata in the development of the inorganic phase during nacre biomineralization, based on experiments using the recombinant form of Pif80. Through interactions with calcium ions, Pif80 participates in the formation of polymer-induced liquid precursor-like amorphous calcium carbonate granules and stabilizes these granules by forming calcium ion-induced coacervates...
August 2017: Science Advances
https://www.readbyqxmd.com/read/28779814/pediatric-flatfoot-pearls-and-pitfalls
#11
REVIEW
Samuel E Ford, Brian P Scannell
Pediatric flatfeet are common, are usually asymptomatic, and typically improve over time as young children age. It is critical to differentiate flexible from rigid flatfeet and to assess for associated Achilles contracture with a careful history, physical examination, and initial radiographs. Although there are limited data, nonsurgical management of symptomatic flatfeet, both flexible and rigid, should be exhausted before considering surgical intervention. If patients fail conservative treatment, surgical management with joint-preserving, deformity-corrective techniques is typically used for pediatric flexible flatfeet in conjunction with deformity-specific soft tissue procedures...
September 2017: Foot and Ankle Clinics
https://www.readbyqxmd.com/read/28779807/calcaneal-osteotomies-pearls-and-pitfalls
#12
REVIEW
Stephen Greenfield, Bruce Cohen
Adult acquired flatfoot deformity is a debilitating condition typically affecting middle-aged patients. The multiple components include hindfoot valgus, first ray elevation, medial soft tissue compromise, and forefoot abduction. As the foot becomes unbalanced, the deformity progresses with repetitive loading and time. Untreated patients often need significant reconstructions or extensive arthrodesis after arthritis and joint contractures present. Medializing calcaneal osteotomy is the workhorse operation for correction of hindfoot valgus, reliably correcting deformity with a relatively low complication risk...
September 2017: Foot and Ankle Clinics
https://www.readbyqxmd.com/read/28776832/neural-interaction-between-risk-sensitivity-and-cognitive-control-predicting-health-risk-behaviors-among-late-adolescents
#13
Jungmeen Kim-Spoon, Kirby Deater-Deckard, Nina Lauharatanahirun, Julee P Farley, Pearl H Chiu, Warren K Bickel, Brooks King-Casas
The developmental period of adolescence is characterized by increasing incidence of health risk behaviors (HRBs). Based on theoretical models that emphasize the moderating role of cognitive control, this study examined how neural correlates of cognitive control and risk sensitivity interact to predict HRBs among late adolescents (17-20 years). Neuroimaging data indicate that risk-related hemodynamic activity in the anterior insula during anticipation of uncertain outcomes predicts HRBs among late adolescents exhibiting greater dorsal anterior cingulate cortex (dACC) activity during a cognitive interference task but not among late adolescents requiring less dACC activity...
September 2017: Journal of Research on Adolescence: the Official Journal of the Society for Research on Adolescence
https://www.readbyqxmd.com/read/28776139/catching-the-zebra-clinical-pearls-and-pitfalls-for-the-successful-diagnosis-of-zollinger-ellison-syndrome
#14
REVIEW
Aaron H Mendelson, Mark Donowitz
Zollinger-Ellison syndrome (ZES) results from an ectopic gastrin-secreting tumor leading to peptic ulcer disease, reflux, and chronic diarrhea. While early recognition portends an excellent prognosis with >80% survival at 15 years, symptoms are often nonspecific making the diagnosis difficult to establish. Diagnosis involves a series of tests, including fasting gastrin, gastric pH, chromogranin A, and secretin stimulation. Performing these tests in the correct sequence and at the proper time is essential to avoid inaccurate results...
August 3, 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/28774486/histopathology-of-tympanic-membranes-from-patients-with-ventilation-tubes
#15
Mehmet Faruk Oktay, Hasan Deniz Tansuker, Hisaki Fukushima, Michael M Paparella, Patricia A Schachern, Sebahattin Cureoglu
OBJECTIVE: To evaluate the histopathologic changes in tympanic membranes (TMs) with ventilation tubes (VTs). METHODS: In this retrospective human temporal bone study our overall study group included 4 subgroups of TMs from deceased donors as follows: 24 with a history of VT insertion for chronic otitis media with effusion (COME-VT); 5 with a history of VT insertion for Meniere's disease (MD-VT); 33 without a history of VT insertion for chronic otitis media with effusion (COME); and 14 without a history of VT insertion for Meniere's disease (MD)...
July 31, 2017: Auris, Nasus, Larynx
https://www.readbyqxmd.com/read/28772357/tissue-partition-and-risk-assessments-of-trace-elements-in-indo-pacific-finless-porpoises-neophocaena-phocaenoides-from-the-pearl-river-estuary-coast-china
#16
Xiyang Zhang, Wenzhi Lin, Ri-Qing Yu, Xian Sun, Yulong Ding, Hailiang Chen, Xi Chen, Yuping Wu
Throughout the last few decades, an increased number of stranded marine mammals, particularly the Indo-Pacific Finless Porpoises (Neophocaena phocaenoides), were observed in the Pearl River Estuary (PRE). As long-lived, apex predators vulnerable to bioaccumulation of contaminants, the tissue residue levels and health risk of trace elements (TEs) in N. phocaenoides from the PRE have been little studied. Eleven typical TEs distributed in skin, liver and kidney tissues were investigated from 25 specimens stranded along the PRE from 2007 to 2015 in the present study...
July 17, 2017: Chemosphere
https://www.readbyqxmd.com/read/28771537/a-spirochaete-is-suggested-as-the-causative-agent-of-akoya-oyster-disease-by-metagenomic-analysis
#17
Tomomasa Matsuyama, Motoshige Yasuike, Atushi Fujiwara, Yoji Nakamura, Tomokazu Takano, Takeshi Takeuchi, Noriyuki Satoh, Yoshikazu Adachi, Yasushi Tsuchihashi, Hideo Aoki, Kazushi Odawara, Shunsuke Iwanaga, Jun Kurita, Takashi Kamaishi, Chihaya Nakayasu
Mass mortality that is acompanied by reddish browning of the soft tissues has been occurring in cultured pearl oyster, Pinctada fucata martensii. The disease is called Akoya oyster disease (AOD). Although spreading pattern of the disease and transmission experiments suggest that the disease is infectious, the causative agent has not yet been identified. We used shotgun and 16S rRNA-based metagenomic analysis to identify genes that are present specifically in affected oysters. The genes found only in diseased oysters were mostly bacterial origin, suggesting that the causative agent was a bacterial pathogen...
2017: PloS One
https://www.readbyqxmd.com/read/28771402/improving-culturally-appropriate-care-using-a-community-based-participatory-research-approach-evaluation-of-a-multicomponent-cultural-competency-training-program-arkansas-2015-2016
#18
Pearl Anna McElfish, Christopher R Long, Brett Rowland, Sarah Moore, Ralph Wilmoth, Britni Ayers
INTRODUCTION: The United States continues to become more racially and ethnically diverse, and racial/ethnic minority communities encounter sociocultural barriers to quality health care, including implicit racial/ethnic bias among health care providers. In response, health care organizations are developing and implementing cultural competency curricula. Using a community-based participatory research (CBPR) approach, we developed and evaluated a cultural competency training program to improve the delivery of culturally appropriate care in Marshallese and Hispanic communities...
August 3, 2017: Preventing Chronic Disease
https://www.readbyqxmd.com/read/28770843/corrigendum-modulating-the-therapeutic-response-of-tumours-to-dietary-serine-and-glycine-starvation
#19
Oliver D K Maddocks, Dimitris Athineos, Eric C Cheung, Pearl Lee, Tong Zhang, Niels J F van den Broek, Gillian M Mackay, Christiaan F Labuschagne, David Gay, Flore Kruiswijk, Julianna Blagih, David F Vincent, Kirsteen J Campbell, Fatih Ceteci, Owen J Sansom, Karen Blyth, Karen H Vousden
This corrects the article DOI: 10.1038/nature22056.
August 2, 2017: Nature
https://www.readbyqxmd.com/read/28769147/applications-of-the-capability-approach-in-the-health-field-a-literature-review
#20
Paul Mark Mitchell, Tracy E Roberts, Pelham M Barton, Joanna Coast
The primary aims of this review are to document capability applications in the health field and to explore the objectives and decision-rules of studies measuring capability more broadly. Relevant studies are identified using a literature search strategy known as "comprehensive pearl growing". All studies with a primary focus on health are assessed individually, whilst a summary narrative analysis of the full review examines the objectives of capability studies. Four distinct groups in the health field are identified in the review: (1) physical activity and diet; (2) patient empowerment; (3) multidimensional poverty and (4) assessments of health and social care interventions...
2017: Social Indicators Research
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