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https://www.readbyqxmd.com/read/27911424/-effectiveness-of-soft-contact-lenses-in-severe-keratoconjunctivitis-sicca-treated-with-local-immunosuppressive-therapy
#1
T N Safonova, O V Gladkova, V I Boev, A A Fedorov
AIM: To study the effectiveness of soft contact lenses in severe keratoconjunctivitis sicca (KCS) treated with local immunosuppressive therapy. MATERIAL AND METHODS: We have examined 41 patients (60 eyes) with severe KCS, including 3 men (4 eyes) and 38 women (56 eyes) aged from 27 to 76 years. Group 1 consisted of 22 persons (30 eyes) who were prescribed cyclosporine А 0.05% instillations 2 times daily, artificial tears, and soft contact lenses. Group 2 consisted of 19 patients (30 eyes) who received instillations of cyclosporine А 0...
2016: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/27904397/antibacterial-activity-of-indian-borage-plectranthus-amboinicus-benth-leaf-extracts-in-food-systems-and%C3%A2-against-natural-microflora-in-chicken-meat
#2
Sandeep Kumar Gupta, Pradeep Singh Negi
The ability of acetone and ethyl acetate extracts of the leaves of a traditional Indian medicinal plant, Indian borage (Plectranthus amboinicus Benth) to prevent spoilage of artificially inoculated model food systems (cabbage and papaya) and natural microflora of chicken meat was evaluated. These extracts were able to reduce the bacterial counts in all food systems; however, the effective concentration varied with the complexity of the system (cabbage<papaya<chicken). A probable mode of action of extracts was investigated by analyzing the changes they cause in bacterial cell wall and leakage of nucleic acid from bacterial cells...
March 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27902886/lateral-gene-transfer-of-p-cresol-and-indole-producing-enzymes-from-environmental-bacteria-to-mastigamoeba-balamuthi
#3
Eva Nývltová, Robert Šuťák, Vojtěch Žárský, Karel Harant, Ivan Hrdý, Jan Tachezy
p-Cresol and indole are volatile biologically active products of the bacterial degradation of tyrosine and tryptophan, respectively. They are typically produced by bacteria in animal intestines, soil and various sediments. Here, we demonstrate that the free-living eukaryote Mastigamoeba balamuthi and its pathogenic relative Entamoeba histolytica produce significant amounts of indole via tryptophanase activity. Unexpectedly, M. balamuthi also produces p-cresol in concentrations that are bacteriostatic to non-p-cresol-producing bacteria...
November 30, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27895415/factoring-the-intestinal-microbiome-into-the-pathogenesis-of-autoimmune-hepatitis
#4
EDITORIAL
Albert J Czaja
The intestinal microbiome is a reservoir of microbial antigens and activated immune cells. The aims of this review were to describe the role of the intestinal microbiome in generating innate and adaptive immune responses, indicate how these responses contribute to the development of systemic immune-mediated diseases, and encourage investigations that improve the understanding and management of autoimmune hepatitis. Alterations in the composition of the intestinal microflora (dysbiosis) can disrupt intestinal and systemic immune tolerances for commensal bacteria...
November 14, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27894756/bioremediation-of-long-term-pcb-contaminated-soil-by-white-rot-fungi
#5
Tatiana Stella, Stefano Covino, Monika Čvančarová, Alena Filipová, Maurizio Petruccioli, Alessandro D'Annibale, Tomáš Cajthaml
The objective of this work was to test the PCB-degrading abilities of two white-rot fungi, namely Pleurotus ostreatus and Irpex lacteus, in real contaminated soils with different chemical properties and autochthonous microflora. In addition to the efficiency in PCB removal, attention was given to other important parameters, such as changes in the toxicity and formation of PCB transformation products. Moreover, structural shifts and dynamics of both bacterial and fungal communities were monitored using next-generation sequencing and phospholipid fatty acid analysis...
November 17, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/27888334/capacity-of-lactic-acid-bacteria-in-immunity-enhancement-and-cancer-prevention
#6
REVIEW
Muhammad Shahid Riaz Rajoka, Junling Shi, Jing Zhu, Dongyan Shao, Qingsheng Huang, Hui Yang, Mingliang Jin
Lactic acid bacteria are associated with the human gastrointestinal tract. They are important for maintaining the balance of microflora in the human gut. An increasing number of published research reports in recent years have denoted the importance of producing interferon-gamma and IgA for treatment of disease. These agents can enhance the specific and nonspecific immune systems that are dependent on specific bacterial strains. The mechanisms of these effects were revealed in this investigation, where the cell walls of these bacteria were modulated by the cytokine pathways, while the whole bacterial cell mediated the host cell immune system and regulated the production of tumor necrosis factors and interleukins...
November 26, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27883159/melatonin-and-hydroxytyrosol-rich-wines-influence-the-generation-of-dna-oxidation-catabolites-linked-to-mutagenesis-after-the-ingestion-of-three-types-of-wine-by-healthy-volunteers
#7
Javier Marhuenda, Sonia Medina, Pedro Martínez-Hernández, Simón Arina, Pilar Zafrilla, Juana Mulero, Hans-Gottfried Genieser, Federico Ferreres, Ángel Gil-Izquierdo
The Mediterranean Diet (MD) has been proved to exert benefits with respect to the maintenance of the redox balance, and wine is a representative component. Bioactive compounds such as polyphenols, melatonin and hydroxytyrosol act as radical scavengers and regulate the oxidation status of organisms. Oxidative damage to DNA yields a large range of end products. The repair of oxidized DNA entails the removal of the useless bases and/or nucleotides as well as the release of circulating nucleotides and nucleosides...
November 24, 2016: Food & Function
https://www.readbyqxmd.com/read/27878718/proinflammatory-cytokines-as-regulators-of-vaginal-microbiota
#8
E A Kremleva, A V Sgibnev
It was shown that IL-1β, IL-8, and IL-6 in concentrations similar to those in the vagina of healthy women stimulated the growth of normal microflora (Lactobacillus spp.) and suppressed the growth and biofilm production by S. aureus and E. coli. On the contrary, these cytokines in higher concentrations typical of vaginal dysbiosis suppressed normal microflora and stimulated the growth of opportunistic microorganisms. TGF-β1 in both doses produced a stimulating effects on study vaginal microsymbionts. It is hypothesized that pro-inflammatory cytokines serve as the molecules of interspecies communication coordinating the interactions of all components of the vaginal symbiotic system...
November 23, 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27876912/synbiotic-biomin-imbo-alters-gut-bacterial-microflora-of-russian-sturgeon-acipenser-guldenstadti-brandt-ratzeburg-1833-in-a-time-dependent-pattern
#9
Mahdi Vaezi, Hossein Khara, Alireza Shenavar
We investigated the efficiency of Synbiotic (Biomin imbo) in alternation and stabilization of gut microflora of Russian sturgeon, Acipenser guldenstadti. For this purpose, five experimental treatments and one control group with three replicates were considered. In treatment groups, fish were fed with diet containing 1 (T1), 1.5 (T2), 2 (T3), 2.5 (T4) and 3 (T5) g Synbiotic/kg diet. In control group, fish were fed without supplement of Synbiotic. Sampling for bacterial studies was conducted at days 20, 40 and 60 after the beginning of the experiment...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27876597/role-of-synbiotics-in-polysaccharide-assisted-colon-targeted-microspheres-of-mesalamine-for-the-treatment-of-ulcerative-colitis
#10
Rupinderjeet Kaur, Monica Gulati, Sachin Kumar Singh
The present study explains the effect of concomitant administration of synbiotics with the polysaccharide based colon targeted delivery system. As the gut microflora get deranged on administration of mesalamine, drug release from such delivery systems is expected to be jeopardised because the release trigger is dependent solely on the colonic microbiota. To overcome this limitation, mesalamine was formulated as microspheres with guar gum and xanthan gum (prebiotics). These were combined with probiotics containing Lactobacillus acidophilus, L...
November 19, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27870289/effects-of-multistrain-probiotics-on-growth-performance-nutrient-digestibility-blood-profiles-faecal-microbial-shedding-faecal-score-and-noxious-gas-emission-in-weaning-pigs
#11
R X Lan, S I Lee, I H Kim
This experiment was conducted to investigate the efficacy of multistrain probiotics in weaning pigs. A total of 125 28-day-old weaning pigs [(Landrace × Yorkshire) × Duroc] with an initial average body weight (BW) of 7.26 ± 0.76 kg were randomly allotted into 5 treatments, 5 replicate pens/treatment with 5 pigs/pen for 42-day experiment. Dietary treatments were as follows: CON, basal diet; PC1, CON + 0.01% multistrain probiotics; PC2, CON + 0.03% multistrain probiotics; PC3, CON + 0.06% multistrain probiotics; PC4, CON + 0...
December 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27860494/salmonella-in-swine-microbiota-interactions
#12
Hyeun Bum Kim, Richard E Isaacson
For the important foodborne pathogen Salmonella enterica to cause disease or persist in pigs, it has evolved an intricate set of interactions between itself, the host, and the indigenous microflora of the host. S. enterica must evade the host's immune system and must also overcome colonization resistance mediated by the pig's indigenous microflora. The inflammatory response against S. enterica provides the bacteria with unique metabolites and is thus exploited by S. enterica for competitive advantage. During infection, changes in the composition of the indigenous microflora occur that have been associated with a breakdown in colonization resistance...
November 9, 2016: Annual Review of Animal Biosciences
https://www.readbyqxmd.com/read/27859814/black-rhinoceros-diceros-bicornis-and-domestic-horse-equus-caballus-hindgut-microflora-demonstrate-similar-fermentation-responses-to-grape-seed-extract-supplementation-in%C3%A2-vitro
#13
N F Huntley, H D Naumann, A L Kenny, M S Kerley
The domestic horse is used as a nutritional model for rhinoceros maintained under human care. The validity of this model for browsing rhinoceros has been questioned due to high prevalence of iron overload disorder (IOD) in captive black rhinoceros (Diceros bicornis), which is associated with high morbidity and mortality. Iron chelators, such as tannins, are under investigation as dietary supplements to ameliorate or prevent IOD in prone species. Polyphenolic compounds variably affect microbial fermentation, so the first objective of this experiment was to evaluate the effects of grape seed extract (GSE; a concentrated source of condensed tannins; CT) on black rhinoceros hindgut fermentation...
November 13, 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27859673/effects-of-dietary-supplementation-with-a-chlorella-by-product-on-the-growth-performance-immune-response-intestinal-microflora-and-intestinal-mucosal-morphology-in-broiler-chickens
#14
H K Kang, S B Park, C H Kim
This study aimed to determine the effect of different dietary levels of a Chlorella by-product (CBP) on the growth performance, immune response, intestinal microflora and intestinal mucosal morphology of broilers. In total, 480 one-day-old broiler chickens were randomly allotted to four dietary treatments with four replicated pens consisting of 30 chicks. The basal diet was formulated to be adequate in energy and nutrients. Three additional diets were prepared by supplementing 25, 50 or 75 g/kg of CBP to the basal diet...
November 17, 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27856942/treponema-species-in-the-subgingival-microflora-of-ovine-periodontitis
#15
A C Borsanelli, T N M Ramos, E Gaetti-Jardim, C M Schweitzer, I S Dutra
No abstract text is available yet for this article.
November 17, 2016: Veterinary Record
https://www.readbyqxmd.com/read/27852539/microbiome-alterations-observed-in-liver-diseases-present-opportunities-for-potential-fecal-transplantation
#16
Ryan D Heath, Fazia Mir, Jamal A Ibdah, Veysel Tahan
Many disease processes lead to chronic liver disease, however, progress has been made regarding common findings amongst these disease processes that may suggest a path forward for treatment. In particular, common alterations in the intestinal microflora of patients with different etiologies of liver disease may provide a clue as to the pathogenesis of these disorders as well a potential therapy. Data is still scant at this point, however, what is available suggests a promising opportunity for future studies to expand upon what has been demonstrated...
November 2016: Turkish Journal of Gastroenterology: the Official Journal of Turkish Society of Gastroenterology
https://www.readbyqxmd.com/read/27845212/microbial-biosensors-to-monitor-the-encapsulation-effectiveness-of-doxorubicin-in-chimeric-advanced-drug-delivery-nano-systems-a-calorimetric-approach
#17
Konstantinos Gardikis, Marco Signorelli, Chiara Ferrario, Alberto Schiraldi, Maria Grazia Fortina, Sophia Hatziantoniou, Costas Demetzos, Dimitrios Fessas
The release of the anticancer drug doxorubicin (DOX) incorporated in a new drug carrier, namely a chimeric nanosystem formed by liposomes and dendrimers, was studied following the influence of the drug on the growth kinetics of the Lactobacillus helveticus bacterium, that would mimic the intestinal microflora. The bacterial growth was followed at 37°C by means of Isothermal Titration Calorimetry (ITC) and the method was assessed to monitor the overall effect of the delivered drug obtaining simple objective parameters to define the encapsulation effectiveness of the system, discriminating dose effects even in cases of very low release...
November 11, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27844263/the-resident-microflora-of-voided-midstream-urine-of-healthy-controls-standard-versus-expanded-urine-culture-protocols
#18
L Coorevits, S Heytens, J Boelens, G Claeys
The workup and interpretation of urine cultures is not always clear-cut, especially for midstream samples contaminated with commensals. Standard urine culture (SUC) protocols are designed in favor of growth of uropathogens at the expense of commensals. In selected clinical situations, however, it is essential to trace fastidious or new uropathogens by expanding the urine culture conditions (EUC). The aim of our study was to map the microflora in midstream urine specimens from healthy controls by means of EUC, in view of the interpretation of bacterial culture results in symptomatic patients...
November 14, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27832762/tryptophan-metabolism-its-relation-to-inflammation-and-stress-markers-and-association-with-psychological-and-cognitive-functioning-tasmanian-chronic-kidney-disease-pilot-study
#19
Naama Karu, Charlotte McKercher, David S Nichols, Noel Davies, Robert A Shellie, Emily F Hilder, Matthew D Jose
BACKGROUND: Adults with chronic kidney disease (CKD) exhibit alterations in tryptophan metabolism, mainly via the kynurenine pathway, due to higher enzymatic activity induced mainly by inflammation. Indoles produced by gut-microflora are another group of tryptophan metabolites related to inflammation and conditions accompanying CKD. Disruptions in tryptophan metabolism have been associated with various neurological and psychological disorders. A high proportion of CKD patients self-report symptoms of depression and/or anxiety and decline in cognitive functioning...
November 10, 2016: BMC Nephrology
https://www.readbyqxmd.com/read/27824363/ability-of-lactobacillus-plantarum-ls-07-to-modify-intestinal-enzymes-activity-in-chronic-diseases-prevention
#20
Emília Hijová, Jozef Kuzma, Ladislav Strojný, Alojz Bomba, Izabela Bertková, Anna Chmelárová, Zdena Hertelyová, Veronika Benetinová, Jana Štofilová, Ľuboš Ambro
The ability of probiotic strain Lactobacillus plantarum LS/07 to modify the activity of intestinal bacterial enzymes - β-glucuronidase (β-GLUCUR), β-galactosidase (β-GAL), and β-glucosidase (β-GLU) in prevention of chronic diseases - cancer, atherosclerosis and dysbiosis was investigated. The male Sprague-Dawley rats were randomly divided into 12 experimental groups: controls groups - C (control), AT (atherosclerotic), CC (carcinogenic), dysbiotic groups - each group in combination with antibiotics (ATB), probiotics groups - in combinatioan with probiotic (PRO) alone, and each group with combination of antibiotic and probiotic (ATB+PRO)...
November 7, 2016: Acta Biochimica Polonica
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