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https://www.readbyqxmd.com/read/28434085/exploring-the-oral-microflora-of-preschool-children
#1
Wen Ren, Qun Zhang, Xuenan Liu, Shuguo Zheng, Lili Ma, Feng Chen, Tao Xu, Baohua Xu
The oral cavity is one of the most important and complicated habitats in our body and supports diverse microbial communities. In this study, we aimed to determine the bacterial diversity and composition of various oral micro-niches. Samples were collected from supragingival plaque, saliva, and tongue coating from 10 preschool children (30 samples total). 16S rRNA gene pyrosequencing dataset generated 314,639 clean reads with an average of 10,488 ± 2,787 reads per sample. The phyla Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Fusobacteria were predominant, accounting for more than 90% of the total sequences...
April 22, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28432990/influence-of-red-mud-on-soil-microbial-communities-application-and-comprehensive-evaluation-of-the-biolog-ecoplate-approach-as-a-tool-in-soil-microbiological-studies
#2
Viktória Feigl, Éva Ujaczki, Emese Vaszita, Mónika Molnár
Red mud can be applied as soil ameliorant to acidic, sandy and micronutrient deficient soils. There are still knowledge gaps regarding the effects of red mud on the soil microbial community. The Biolog EcoPlate technique is a promising tool for community level physiological profiling. This study presents a detailed evaluation of Biolog EcoPlate data from two case studies. In experiment "A" red mud from Ajka (Hungary) was mixed into acidic sandy soil in soil microcosms at 5-50 w/w%. In experiement "B" red mud soil mixture was mixed into low quality subsoil in a field experiment at 5-50 w/w%...
April 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28431945/in-vitro-fermentation-behaviors-of-fucosylated-chondroitin-sulfate-from-pearsonothuria-graeffei-by-human-gut-microflora
#3
Chao-Yang Wei, Ning-Bo Liao, Yu Zhang, Xing-Qian Ye, Shan Li, Ya-Qin Hu, Dong-Hong Liu, Robert J Linhardt, Xin Wang, Shi-Guo Chen
A fucosylated chondroitin sulfate (FCS-pg) with highly repeated structure from Pearsonothuria graeffei was subjected to a in vitro fermentation model to investigate its fermentability and effects on human gut microflora. High performance liquid chromatography (HPLC) measurement found FCS-pg can be fermented to short chain fatty acids (SCFAs) by gut microflora from partial human fecal samples. 16S rRNA gene-based polymerase chain reaction-based denaturing gradient gel electrophoresis (PCR-DGGE) profiling and real-time quantitative PCR analysis showed that FCS-pg mainly increased the proportions of Clostridium cluster XI, Bacteriodes prevotella group, Bifidobacterium genus, Clostridium cluster I and Clostridium cluster XIVab, whereas the numbers of the Enterobacteriaceae and Lactobacillus decreased...
April 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28429590/palladium-catalyzed-n-2-aminophenyl-acetamide-assisted-ortho-arylation-of-substituted-benzamides-application-to-the-synthesis-of-urolithins-b-m6-and-m7
#4
M Damoder Reddy, Alexandra N Blanton, E Blake Watkins
Pd-catalyzed, selective, monoarylation of ortho-C-H bonds of various benzamides with aryl/heteroaryl iodides has been realized using N-(2-aminophenyl)acetamide (APA) as a new bidentate directing group for the first time. The reaction was tolerant of a wide range of functional groups, and a variety of biaryl amide derivatives were successfully prepared in good to moderate yield. The utilization of N-(2-aminophenyl)acetamide as a novel directing group, Mn(OAc)2 as a co-oxidant (silver free reaction conditions), and absolute ortho-monoaryl selectivity are notable features of this reaction...
April 21, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28429365/a-randomized-clinical-trial-about-presence-of-pathogenic-microflora-and-risk-of-peri-implantitis-comparison-of-two-different-types-of-implant-abutment-connections
#5
F Mencio, F De Angelis, P Papi, D Rosella, G Pompa, S Di Carlo
OBJECTIVE: The aim of this in vivo study was to evaluate two different types of implant-abutment connections: screwed connection and cemented connection, analyzing peri-implant bacteria microflora as well as other clinical parameters. PATIENTS AND METHODS: Twenty implants were selected, inserted in 20 patients, 10 with a screwed implant-abutment connection (Group 1) and 10 with a cemented implant-abutment connection (Group 2). The peri-implant microflora was collected, after at least 360 days from the prosthetic rehabilitation, using paper points inserted in peri-implant sulcus for 30 s...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28429209/analysis-of-gut-microbiota-in-patients-with-parkinson-s-disease
#6
V A Petrov, I V Saltykova, I A Zhukova, V M Alifirova, N G Zhukova, Yu B Dorofeeva, A V Tyakht, B A Kovarsky, D G Alekseev, E S Kostryukova, Yu S Mironova, O P Izhboldina, M A Nikitina, T V Perevozchikova, E A Fait, V V Babenko, M T Vakhitova, V M Govorun, A E Sazonov
Gut microbiota of patients with Parkinson's disease and healthy volunteers was analyzed by the method of high throughput 16S rRNA sequencing of bacterial genomes. In patients with Parkinson's diseases, changes in the content of 9 genera and 15 species of microorganisms were revealed: reduced content of Dorea, Bacteroides, Prevotella, Faecalibacterium, Bacteroides massiliensis, Stoquefichus massiliensis, Bacteroides coprocola, Blautia glucerasea, Dorea longicatena, Bacteroides dorei, Bacteroides plebeus, Prevotella copri, Coprococcus eutactus, and Ruminococcus callidus, and increased content of Christensenella, Catabacter, Lactobacillus, Oscillospira, Bifidobacterium, Christensenella minuta, Catabacter hongkongensis, Lactobacillus mucosae, Ruminococcus bromii, and Papillibacter cinnamivorans...
April 20, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28425301/development-of-a-novel-rationally-designed-antibiotic-to-inhibit-a-nontraditional-bacterial-target
#7
Pavel Dibrov, Elena Dibrov, Thane G Maddaford, Melissa Kenneth, Jordan Nelson, Craig Resch, Grant N Pierce
The search for new nontraditional targets is a high priority in antibiotic design today. Bacterial membrane energetics based on sodium ion circulation offers potential alternative targets. The present work identifies the Na(+)-translocating NADH:ubiquinone oxidoreductase (Na(+)-NQR), a key respiratory enzyme in many microbial pathogens, as indispensible for the Chlamydia trachomatis infectious process. Infection by Chlamydia trachomatis significantly increased first H(+) and then Na(+) levels within the host mammalian cell...
April 20, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28425267/modifications-of-mice-gut-microflora-following-oral-consumption-oflactobacillus-acidophilus-and-bifidobacterium-bifidum-probiotics
#8
Hossein Khavari-Daneshvar, Maryam Mosavi, Hamid Khodayari, Ebrahim Rahimi, Peyman Ranji, Amir Hossein Mohseni, Reyhaneh Mahmudian, Farzad Shidfar, Shahram Agah, Ali Mohammad Alizadeh
BACKGROUND/AIM: Thirty male BALB/c mice were equally divided into three groups: control, L. acidophilus, and B. bifidum for the assessment of the probiotics' stability in the gut microflora. MATERIALS AND METHODS: First, the gut microflora of the mice was checked every 3 days (days 3, 6, 9, and 12) without probiotic consumption, and then the mice were daily given orally 1.5 g of probiotics in 30 cc of drinking water. The consumption of probiotics was then stopped for recovery and then the consumption continued for 5 months...
April 18, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28419329/effects-of-the-inclusion-of-a-bacillus-direct-fed-microbial-on-performance-parameters-bone-quality-recovered-gut-microflora-and-intestinal-morphology-in-broilers-consuming-a-grower-diet-containing-corn-distillers-dried-grains-with-solubles
#9
J D Latorre, X Hernandez-Velasco, J L Vicente, R Wolfenden, B M Hargis, G Tellez
Distillers dried grains with solubles (DDGS) have increasingly been used in poultry diets as a consequence of rising grain costs. Some, but not all, sources of DDGS have a variable compositional value, and a high inclusion of this by-product could be considered a risk factor for presentation of enteric diseases. Presently, 2 experiments were conducted using a starter corn-soybean diet (zero to 7 d) and a corn-DDGS-soybean grower diet (8 to 28 d) with or without inclusion of a Bacillus-direct-fed microbial (DFM)...
April 17, 2017: Poultry Science
https://www.readbyqxmd.com/read/28418364/-evolution-of-complex-treatment-of-patients-with-non-specific-pleural-empyema
#10
O O Yasnogorodsky, A M Shulutko, T P Pinchuk, E G Gandybina, A S Kachikin, F N Nasirov, A Yu Moiseev, S E Gryaznov
AIM: To analyze long-term own experience of NPE treatment in view of evolution of surgical sanitation of pleural cavity. MATERIAL AND METHODS: The analysis included 5115 patients with NPE for the last 39 years (1977-2015). Morbidity, features of microflora of purulent exudate, changes in the structure of surgical methods were assessed. The role of computed tomography in the diagnostic algorithm and treatment of NPE was studied. RESULTS: The evolution of NPE surgical management includes introduction of video technologies, thermal surgical instruments and widespread use of computed tomography in the diagnosis of pleural empyema...
2017: Khirurgiia
https://www.readbyqxmd.com/read/28411687/oral-administration-of-red-ginseng-powder-fermented-with-probiotic-alleviates-the-severity-of-dextran-sulfate-sodium-induced-colitis-in-a-mouse-model
#11
Sun-Hee Jang, Jisang Park, Sae-Hae Kim, Kyung-Min Choi, Eun-Sil Ko, Jeong-Dan Cha, Young-Ran Lee, Hyonseok Jang, Yong-Suk Jang
Red ginseng is a well-known alternative medicine with anti-inflammatory activity. It exerts pharmacological effects through the transformation of saponin into metabolites by intestinal microbiota. Given that intestinal microflora vary among individuals, the pharmacological effects of red ginseng likely vary among individuals. In order to produce homogeneously effective red ginseng, we prepared probiotic-fermented red ginseng and evaluated its activity using a dextran sulfate sodium (DSS)-induced colitis model in mice...
March 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28409819/spermicidal-efficacy-of-vrp-a-synthetic-cationic-antimicrobial-peptide-inducing-apoptosis-and-membrane-disruption
#12
Prasanta Ghosh, Arpita Bhoumik, Sudipta Saha, Sandipan Mukherjee, Sarfuddin Azmi, Jimut Kanti Ghosh, Sandhya Rekha Dungdung
Presently available contraceptives are mostly hormonal or detergent in nature with numerous side effects like irritation, lesion, inflammation in vagina, alteration of body homeostasis etc. Antimicrobial peptides with spermicidal activity but without adverse effects may be suitable alternatives. In the present study spermicidal activity of a cationic antimicrobial peptide VRP on human spermatozoa has been elucidated. Progressive forward motility of human spermatozoa was instantly stopped after 100 µM VRP treatment and at 350 µM all kinds of sperm motility ceased within 20 seconds as assessed by the Sander-Cramer assay...
April 14, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28409536/improved-quality-of-a-vitamin-b12-fortified-ready-to-blend-fresh-cut-mix-salad-with-chitosan
#13
Francisco Artés-Hernández, Anna Carolina Formica-Oliveira, Francisco Artés, Ginés Benito Martínez-Hernández
A vitamin B12-fortified (0.25 mg L(-1)) chitosan (10 g L(-1)) coating was applied to a ready-to-blend fresh-cut salad including melon, pineapple and carrot, which was stored at 5 ℃ up to nine days. Uncoated samples were used as control while the vitamin B12 effect was compared to non-fortified chitosan-coated samples. Beverages were prepared on blending days 0, 4, 7 and 9 with subsequent storage at 5 ℃ for 0, 24 and 48 h. Physicochemical quality of fresh blended beverages was well preserved throughout storage...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/28408691/whole-genome-sequence-of-staphylococcus-hominis-strain-j31-isolated-from-healthy-human-skin
#14
Rosanna Coates-Brown, Malcolm J Horsburgh
We report here the first whole-genome sequence of a skin-associated strain of Staphylococcus hominis determined using the PacBio long-read sequencing platform. S. hominis is a major commensal of the skin microflora. This genome sequence adds to our understanding of this species and will aid studies of gene traffic between staphylococci.
April 13, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28407420/dental-biofilm-infections-an-update
#15
REVIEW
Tove Larsen, Nils-Erik Fiehn
Teeth are colonized by oral bacteria from saliva containing more than 700 different bacterial species. If removed regularly, the dental biofilm mainly comprises oral streptococci and is regarded as resident microflora. But if left undisturbed, a complex biofilm containing up to 100 bacterial species at a site will build up and may eventually cause development of disease. Depending on local ecological factors, the composition of the dental biofilm may vary considerably. With access to excess carbohydrates, the dental biofilm will be dominated by mainly gram-positive carbohydrate-fermenting bacteria causing demineralization of teeth, dental caries, which may further lead to inflammation and necrosis in the pulp and periapical region, i...
April 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28406692/effect-of-pre-and-post-weaning-high-fat-dietary-manipulation-on-intestinal-microflora-and-alkaline-phosphatase-activity-in-male-rats
#16
Z Šefčíková, D Bujňáková
We investigated the impact of a high-fat (HF) diet during pre- and post-weaning periods on the intestinal microbiota and alkaline phosphatase (AP) activity in male rats. Nutrition from birth was influenced by feeding rat dams with either a standard or HF diet. After weaning male pups nursed by control dams continued on a standard diet (CC) or HF diet (C->HF), while offspring nursed by HF dams continued on HF diet (HF) or standard diet (HF->C). The numbers of Bacteriodes/Prevotella (BAC) and Lactobacillus/Enterococcus (LAB) in the gut were determined by FISH technique...
April 12, 2017: Physiological Research
https://www.readbyqxmd.com/read/28402706/optimum-operating-room-environment-for-the-prevention-of-surgical-site-infections
#17
Sara Gaines, James N Luo, Jack Gilbert, Olga Zaborina, John C Alverdy
BACKGROUND: Surgical site infections (SSI), whether they be incisional or deep, can entail major morbidity and death to patients and additional cost to the healthcare system. A significant amount of effort has gone into optimizing the surgical patient and the operating room environment to reduce SSI. METHODS: Relevant guidelines and literature were reviewed. RESULTS: The modern practice of surgical antisepsis involves the employment of strict sterile techniques inside the operating room...
April 12, 2017: Surgical Infections
https://www.readbyqxmd.com/read/28399595/can-the-pessary-use-modify-the-vaginal-microbiological-flora-a-cross-sectional-study
#18
Suelene Costa de Albuquerque Coelho, Paulo César Giraldo, Juliana Oquendo Florentino, Edilson Benedito de Castro, Luiz Gustavo Oliveira Brito, Cássia Raquel Teatin Juliato
Introduction Vaginal pessary is used as a conservative treatment for pelvic organ prolapse (POP). Some studies have shown that common complaints of its use may include vaginal discomfort and increased vaginal discharge. Scant information is available about the microflora status after using this device. Objective To determine if the usage of vaginal pessary can interfere with the vaginal environment. Methods A cross-sectional study was performed from March of 2014 to July of 2015 including 90 women with POP...
April 2017: Revista Brasileira de Ginecologia e Obstetrícia
https://www.readbyqxmd.com/read/28398869/the-in-vitro-and-in-vivo-effect-of-carvacrol-in-preventing-campylobacter-infection-colonization-and-in-improving-productivity-of-chicken-broilers
#19
Carmel Kelly, Ozan Gundogdu, Gratiela Pircalabioru, Ada Cean, Pam Scates, Mark Linton, Laurette Pinkerton, Elizabeth Magowan, Lavinia Stef, Eliza Simiz, Ioan Pet, Sharon Stewart, Richard Stabler, Brendan Wren, Nick Dorrell, Nicolae Corcionivoschi
The current trend in reducing the antibiotic usage in animal production imposes urgency in the identification of novel biocides. The essential oil carvacrol, for example, changes the morphology of the cell and acts against a variety of targets within the bacterial membranes and cytoplasm, and our in vitro results show that it reduces adhesion and invasion of chicken intestinal primary cells and also biofilm formation. A trial was conducted to evaluate the effects of dietary supplementation of carvacrol at four concentrations (0, 120, 200, and 300 mg/kg of diet) on the performance of Lactobacillus spp...
February 27, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28398088/effects-of-fermented-cottonseed-meal-on-the-growth-performance-gastrointestinal-microflora-population-and-small-intestinal-morphology-in-broiler-chickens
#20
V Jazi, F Boldaji, B Dastar, S R Hashemi, A Ashayerizadeh
1. This experiment was conducted to evaluate the effects of replacing dietary cottonseed meal (CSM) or fermented cottonseed meal (FCSM) for soybean meal (SBM) on growth performance, carcass characteristics, gastrointestinal microbial populations, and intestinal morphology of broiler chickens. 2. CSM was fermented with Bacillus subtilis, Aspergillus niger and Aspergillus oryzae for 7 days. A total of 300 one-d-old male Ross 308 broiler chickens were used in a 42-d experiment in which the birds were randomly allotted to one of 5 dietary treatments (containing 0, 10 and 20 percent CSM or FCSM) in a completely randomised design...
April 11, 2017: British Poultry Science
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