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https://www.readbyqxmd.com/read/28339714/effect-of-whole-yeast-cell-product-supplementation-citristim%C3%A2-on-immune-responses-and-cecal-microflora-species-in-pullet-and-layer-chickens-during-an-experimental-coccidial-challenge
#1
Ashley D Markazi, Victor Perez, Mamduh Sifri, Revathi Shanmugasundaram, Ramesh K Selvaraj
Three separate experiments were conducted to study the effects of whole yeast cell product supplementation in pullets and layer hens. Body weight gain, fecal and intestinal coccidial oocyst counts, cecal microflora species, cytokine mRNA amounts, and CD4+ and CD8+ T-cell populations in the cecal tonsils were analyzed following an experimental coccidial infection. In Experiment I, day-old Leghorn layer chicks were fed 3 experimental diets with 0, 0.1, or 0.2% whole yeast cell product (CitriStim®, ADM, Decatur, IL)...
February 21, 2017: Poultry Science
https://www.readbyqxmd.com/read/28335485/intra-amniotic-administration-of-raffinose-and-stachyose-affects-the-intestinal-brush-border-functionality-and-alters-gut-microflora-populations
#2
Sarina Pacifici, Jaehong Song, Cathy Zhang, Qiaoye Wang, Raymond P Glahn, Nikolai Kolba, Elad Tako
This study investigates the effectiveness of two types of prebiotics-stachyose and raffinose-which are present in staple food crops that are widely consumed in regions where dietary Fe deficiency is a health concern. The hypothesis is that these prebiotics will improve Fe status, intestinal functionality, and increase health-promoting bacterial populations in vivo (Gallus gallus). By using the intra-amniotic administration procedure, prebiotic treatment solutions were injected in ovo (day 17 of embryonic incubation) with varying concentrations of a 1...
March 19, 2017: Nutrients
https://www.readbyqxmd.com/read/28334549/limitations-of-immunomagnetic-separation-for-detection-of-the-top-seven-serogroups-of-shiga-toxin-producing-escherichia-coli
#3
J Hallewell, T Alexander, T Reuter, K Stanford
Shiga toxin-producing Escherichia coli (STEC) strains are foodborne pathogens that negatively impact human health and compromise food safety. Serogroup O157 is the most frequently isolated and studied STEC serogroup, but six others (O26, O45, O103, O111, O121, and O145) have also been identified as significant sources of human disease and collectively have been referred to as the "top six" pathogenic serogroups. Because detection methods for non-O157 serogroups are not yet refined, the objective of this study was to compare the effectiveness of immunomagnetic separation (IMS) for recovery of serogroup O157 isolates with that for each of the top six E...
March 9, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28330225/metagenomics-analysis-of-microbial-communities-associated-with-a-traditional-rice-wine-starter-culture-xaj-pitha-of-assam-india
#4
Sudipta Sankar Bora, Jyotshna Keot, Saurav Das, Kishore Sarma, Madhumita Barooah
This is the first report on the microbial diversity of xaj-pitha, a rice wine fermentation starter culture through a metagenomics approach involving Illumine-based whole genome shotgun (WGS) sequencing method. Metagenomic DNA was extracted from rice wine starter culture concocted by Ahom community of Assam and analyzed using a MiSeq(®) System. A total of 2,78,231 contigs, with an average read length of 640.13 bp, were obtained. Data obtained from the use of several taxonomic profiling tools were compared with previously reported microbial diversity studies through the culture-dependent and culture-independent method...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28319798/cold-plasma-activated-hydrogen-peroxide-aerosol-inactivates-escherichia-coli-o157-h7-salmonella-typhimurium-and-listeria-innocua-and-maintains-quality-of-grape-tomato-spinach-and-cantaloupe
#5
Yunbin Jiang, Kimberly Sokorai, Georgios Pyrgiotakis, Philip Demokritou, Xihong Li, Sudarsan Mukhopadhyay, Tony Jin, Xuetong Fan
The purpose of this study was to investigate the efficacy of aerosolized hydrogen peroxide in inactivating bacteria and maintaining quality of grape tomatoes, baby spinach leaves and cantaloupes. Stem scars and smooth surfaces of tomatoes, spinach leaves, and cantaloupe rinds, inoculated with Escherichia coli O157:H7, Salmonella Typhimurium and Listeria innocua, were treated for 45s followed by additional 30min dwell time with hydrogen peroxide (7.8%) aerosols activated by atmospheric cold plasma. Non-inoculated samples were used to study the effects on quality and native microflora populations...
March 10, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28317832/-variations-of-oral-fluid-microbiota-in-healthy-children-and-adolescents
#6
B N Davydov, A M Samoukina, E S Mikhailova, O A Gavrilova, Yu A Alekseeva
The aim of the study was to establish the bacterial and viral associations of saliva and the immune resistance in 127 healthy people of different ages. The analysis sorted out three variants of oral fluid microbiota differing by certain combination of indigenous and facultative microflora. It was found that with age there is a significant increase in the number of adolescents with the third variant of the microbiota, characterized by a decrease in the number of indigenous microflora and increase in opportunistic microorganisms and viruses...
2017: Stomatologii︠a︡
https://www.readbyqxmd.com/read/28317217/effects-of-a-protected-inclusion-of-organic-acids-and-essential-oils-as-antibiotic-growth-promoter-alternative-on-growth-performance-intestinal-morphology-and-gut-microflora-in-broilers
#7
Yanli Liu, Xin Yang, Hongliang Xin, Si Chen, Chengbo Yang, Yulan Duan, Xiaojun Yang
This experiment was conducted to investigate the effects of protected essential oils and organic acids mixture on poultry feeding. A total of 450 1-day-old Cobb 500 chicks were randomly allotted into three treatments with six replicates. Birds were offered a basal diet (C), basal diet with 0.15 g/kg enramycin premix (A) and basal diet with 0.30 g/kg protected essential oils and organic acids mixture product (P). The results showed that protected essential oils and organic acids mixture supplementation reduced average daily feed intake and ratio of feed to gain (F/G) at 22-42 days of age, and F/G during 1-42 days of age also declined (P < 0...
March 20, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28316999/does-the-gut-microbiota-influence-immunity-and-inflammation-in-multiple-sclerosis-pathophysiology
#8
Monika Adamczyk-Sowa, Aldona Medrek, Paulina Madej, Wirginia Michlicka, Pawel Dobrakowski
Aim. Evaluation of the impact of gut microflora on the pathophysiology of MS. Results. The etiopathogenesis of MS is not fully known. Gut microbiota may be of a great importance in the pathogenesis of MS, since recent findings suggest that substitutions of certain microbial population in the gut can lead to proinflammatory state, which can lead to MS in humans. In contrast, other commensal bacteria and their antigenic products may protect against inflammation within the central nervous system. The type of intestinal flora is affected by antibiotics, stress, or diet...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28316889/functional-and-health-promoting-inherent-attributes-of-enterococcus-hirae-f2-as-a-novel-probiotic-isolated-from-the-digestive-tract-of-the-freshwater-fish-catla-catla
#9
Mohd Adnan, Mitesh Patel, Sibte Hadi
BACKGROUND: Probiotic microorganisms are gaining global importance because of their use in the preparation of a nutraceutical or in the treatment of infections. As per the health industry demand, there is an urgent need for exploring new indigenous probiotic strains with its specific origin due to variation in gut microflora, different food habits and specific host-microbial interactions. The main objective of the present study was to isolate and identify a novel probiotic Enterococcus strain from the gut of Catla catla fish and evaluate its potentiality as a potent probiotic...
2017: PeerJ
https://www.readbyqxmd.com/read/28316482/monitoring-of-bioluminescent-lactobacillus-plantarum-in-a-complex-food-matrix
#10
Gi-Seong Moon, Arjan Narbad
A bioluminescent Lactobacillus plantarum (pLuc2) strain was constructed. The luminescent signal started to increase during the early exponential phase and reached its maximum in the mid-exponential phase in a batch culture of the strain. The signal detection sensitivity of the strain was the highest in PBS (phosphate buffered saline), followed by milk and MRS broth, indicating that the sensitivity was influenced by the matrix effect. The strain was used in millet seed fermentation which has a complex matrix and native lactic acid bacteria (LAB)...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/28298816/effect-of-gallium-arsenic-laser-on-photosensitized-periodontopathic-anaerobic-organisms-an-in-vitro-study
#11
Setu Mathur, Shaila Veerappa Kothiwale, Buddhi Prakash Nag, Tanu Mathur, Ashoka Bhansali, Rohit Kumar Khatri
BACKGROUND: The mainstay of periodontal therapy is mechanical removal of subgingival plaque. There is considerable interest in supplementing it with the use of antibiotics and antiseptics. Many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. Hence, alternate means of killing subgingival bacteria are clearly desirable. One such method is the use of laser...
July 2016: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/28298696/the-impact-of-different-barrel-sanitation-approaches-on-the-spoilage-microflora-and-phenols-composition-of-wine
#12
Raffaele Guzzon, Manfred Bernard, Chiara Barnaba, Daniela Bertoldi, Konrad Pixner, Roberto Larcher
Careful control of spoilage microflora inside wine containers is a key issue during winemaking. To date, attention has been paid to the development of an effective protocol for the eradication of spoilage agents, especially Brettanomyces, from barrels. Few studies have taken into account the modifications caused by sanitation treatments in wine and wood barrels. In the present study the effects of two sanitation treatments (ozone and sodium hydroxide) on barrel spoilage microflora and the composition of the wine stored inside them were evaluated...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28298675/lactic-acid-as-potential-substitute-of-acetic-acid-for-dissolution-of-chitosan-preharvest-application-to-butterhead-lettuce
#13
María Gabriela Goñi, Bárbara Tomadoni, Sara Inés Roura, María Del Rosario Moreira
Chitosan must be dissolved in acid solution to activate its antimicrobial properties. The objectives of present study were to determine whether acetic and lactic acids used to dissolve chitosan would influence its effectiveness to control the native microflora of Butterhead lettuce at harvest and during postharvest storage (7-8 °C, 5 days). Chitosan was applied as a SINGLE DOSE (14, 10, 7, 3 or 0 days previous to harvest) or in SUCCESSIVE DOSES (at 14 + 10 + 7+3 + 0 days prior to harvest). Although chitosan in acetic acid showed antimicrobial activity, treated plants showed dried brown stains which significantly reduced sensorial quality...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28296922/the-potential-role-of-osteopontin-in-the-maintenance-of-commensal-bacteria-homeostasis-in-the-intestine
#14
Koyu Ito, Akira Nakajima, Yuji Fukushima, Keiichiro Suzuki, Keiko Sakamoto, Yoko Hamazaki, Kouetsu Ogasawara, Nagahiro Minato, Masakazu Hattori
Osteopontin (Opn), a multifunctional extracellular matrix protein, is implicated in the pathogenesis of various inflammatory disorders. Under physiologic conditions, its expression is restricted to certain tissues including bone and kidney tubule. However, cellular activation during disease development induces Opn expression in various immune cells. In this study, using Opn-EGFP knock-in (KI) mice we found that CD8α+ T cells in the intestinal tissues, including Peyer's patch, lamina propria and epithelium, express Opn under steady state conditions...
2017: PloS One
https://www.readbyqxmd.com/read/28296209/the-urinary-1-h-nmr-metabolomics-profile-of-an-italian-autistic-children-population-and-their-unaffected-siblings
#15
Milena Lussu, Antonio Noto, Alice Masili, Andrea C Rinaldi, Angelica Dessì, Maria De Angelis, Andrea De Giacomo, Vassilios Fanos, Luigi Atzori, Ruggiero Francavilla
Autism spectrum disorders (ASD) make a dishomogeneous group of psychiatric diseases having either genetic and environmental components, including changes of the microbiota. The rate of diagnosis, based on a series of psychological tests and observed behavior, dramatically increased in the past few decades. Currently, no biological markers are available and the pathogenesis is not defined. The purpose of this study was to evaluate the potential use of (1) H-NMR metabolomics to analyze the global biochemical signature of ASD patients (n = 21) and controls (n = 21), these being siblings of autistic patients...
March 11, 2017: Autism Research: Official Journal of the International Society for Autism Research
https://www.readbyqxmd.com/read/28295656/effect-of-dietary-polyphenol-rich-grape-seed-on-growth-performance-antioxidant-capacity-and-ileal-microflora-in-broiler-chicks
#16
S H Abu Hafsa, S A Ibrahim
In this study, we examined the effect of dietary supplementation with grape seed (GS) on the performance, carcass traits, plasma biochemistry, antioxidant status and ileal microflora in broilers. Experiment diets included a control diet (without additive) and three levels of GS powder (10, 20 and 40 g/kg of diet). Each diet was fed to a total of 300 one-day-old Cobb-500 chicks for 42 days. The addition of 20 g/kg of GS to the basal diet increased final body weight and body weight gain, improved the feed conversion ratio and did not affect feed intake...
March 15, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28293637/identification-of-candidate-genes-related-to-inflammatory-bowel-disease-using-minimum-redundancy-maximum-relevance-incremental-feature-selection-and-the-shortest-path-approach
#17
Fei Yuan, Yu-Hang Zhang, Xiang-Yin Kong, Yu-Dong Cai
Identification of disease genes is a hot topic in biomedicine and genomics. However, it is a challenging problem because of the complexity of diseases. Inflammatory bowel disease (IBD) is an idiopathic disease caused by a dysregulated immune response to host intestinal microflora. It has been proven to be associated with the development of intestinal malignancies. Although the specific pathological characteristics and genetic background of IBD have been partially revealed, it is still an overdetermined disease and the blueprint of all genetic variants still needs to be improved...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28290327/effect-of-high-hydrostatic-pressure-processing-on-the-background-microbial-loads-and-quality-of-cantaloupe-puree
#18
Sudarsan Mukhopadhyay, Kimberly Sokorai, Dike Ukuku, Xuetong Fan, Vijay Juneja
The objective of this study was to investigate and evaluate the effects of high hydrostatic pressure (HHP) applied to cantaloupe puree (CP) on microbial loads and product quality during storage for 10days at 4°C. Freshly prepared, double sealed and double bagged CP (ca. 5g) was pressure treated at 300, 400 and 500MPa at 8°C and 15°C for 5min. Microflora populations, soluble solid content, pH, color, antioxidant activity, appearance and aroma were measured at 1, 6, and 10d of storage. Results showed that high pressure treatment of 300MPa (8°C and 15°C) resulted in reduction of total aerobic plate count from 3...
January 2017: Food Research International
https://www.readbyqxmd.com/read/28285374/antibiotic-mixture-effects-on-growth-of-the-leaf-shredding-stream-detritivore-gammarus-fossarum
#19
Mirco Bundschuh, Torsten Hahn, Mark O Gessner, Ralf Schulz
Pharmaceuticals contribute greatly to human and animal health. Given their specific biological targets, pharmaceuticals pose a significant environmental risk by affecting organisms and ecosystem processes, including leaf-litter decomposition. Although litter decomposition is a central process in forest streams, the consequences of exposure to pharmaceuticals remain poorly known. The present study assessed the impact of antibiotics as an important class of pharmaceuticals on the growth of the leaf-shredding amphipod Gammarus fossarum over 24 days...
March 11, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28285115/teriyaki-sauce-with-carvacrol-or-thymol-effectively-controls-escherichia-coli-o157-h7-listeria-monocytogenes-salmonella-typhimurium-and-indigenous-flora-in-marinated-beef-and-marinade
#20
Hyeree Moon, Nam Hee Kim, Soon Han Kim, Younghoon Kim, Jee Hoon Ryu, Min Suk Rhee
An effective bactericidal cold-marinating method for beef products is described, exploiting the synergism between soy sauce and natural compounds (carvacrol, CV or thymol, TM) to reduce microbiological risks. Beef slices inoculated with Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella Typhimurium (3.1-3.5logCFU/g) were marinated in a teriyaki sauce with or without CV and TM (0.3 and 0.5%). After 1, 3, and 7days at 4°C, indigenous microflora population, color, lipid oxidation, marinade uptake, and pH of marinated beef and leftover marinade samples were examined...
March 6, 2017: Meat Science
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