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https://www.readbyqxmd.com/read/28810610/microbial-investigations-in-throat-swab-and-tracheal-aspirate-specimens-are-beneficial-to-predict-the-corresponding-endotracheal-tube-biofilm-flora-among-intubated-neonates-with-ventilator-associated-pneumonia
#1
Yun Pan, Lizhong Du, Qing Ai, Sijie Song, Xiaoli Tang, Danping Zhu, Jialin Yu
Ventilator-associated pneumonia (VAP) is a common nosocomial infection in neonatal intensive care units with high morbidity and mortality. Bacterial biofilm in the endotracheal tube (ET) provides a notable and persistent source of pathogens that may cause VAP, and thus is important for VAP detection. However, during intubation microbial investigations in ET, samples are unavailable due to the infeasibility of collecting ET samples during intubation of neonates. It is therefore of great importance to find alternative sources of samples that can help identify the ET biofilm flora...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28794893/effect-of-dietary-sugar-beet-pulp-supplementation-on-growth-performance-nutrient-digestibility-fecal-microflora-blood-profiles-and-diarrhea-incidence-in-weaning-pigs
#2
C L Yan, H S Kim, J S Hong, J H Lee, Y G Han, Y H Jin, S W Son, S H Ha, Y Y Kim
BACKGROUND: In 2006, the European Union (EU) has decided to forbid use of antibiotics as growth promoters. Although many researches had been conducted about fiber source as alternatives of antibiotics, there are still lack of reports in the literature about the optimum level of sugar beet pulp supplementation, affecting growth performance and nutrient digestibility in weaning pigs. Therefore, different level of sugar beet pulp was added to diets to determine the effects of sugar beet pulp supplementation on growth performance, nutrient digestibility, fecal microflora, blood profile and incidence of diarrhea in weaning pigs...
2017: Journal of Animal Science and Technology
https://www.readbyqxmd.com/read/28783552/a-meta-analysis-of-randomized-trials-assessing-the-effects-of-probiotic-preparations-on-oral-candidiasis-in-the-elderly
#3
REVIEW
Ruixue Ai, Jiao Wei, Danhua Ma, Lu Jiang, Hongxia Dan, Yu Zhou, Ning Ji, Xin Zeng, Qianming Chen
OBJECTIVE: Oral candidiasis is the most common fungal infection and can be attributed in part to dysbiosis, an imbalance in the resident oral microflora. Therefore, probiotics, which counter pathogenic microorganisms through competitive, antagonistic, and immunological effects, have been used by some clinicians. To date, the effect of probiotics in preventing oral candidiasis in the elderly is controversial. A systematic review that summarizes and critically appraises the available clinical trials is therefore necessary...
April 30, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28771569/disruption-in-the-cecal-microbiota-of-chickens-challenged-with-clostridium-perfringens-and-other-factors-was-alleviated-by-bacillus-licheniformis-supplementation
#4
Yicen Lin, Shuai Xu, Dong Zeng, Xueqin Ni, Mengjia Zhou, Yan Zeng, Hesong Wang, Yi Zhou, Hui Zhu, Kangcheng Pan, Guangyao Li
Clostridium perfringens can induce necrotic enteritis of chickens, which causes large economic losses every year. Bacillus licheniformis, a probiotic, can inhibit the growth of pathogenic bacteria such as Clostridium perfringens, thereby improving the health status of chickens. However, from a microbial ecology perspective, the mechanisms by which alterations to the gut microbiota improve health remain unknown. In this study, we used Illumina MiSeq sequencing to investigate the cecal microbiota of a negative control group (NC), a C...
2017: PloS One
https://www.readbyqxmd.com/read/28764082/biotransformation-and-metabolism-of-three-mulberry-anthocyanin-monomers-by-rat-gut-microflora
#5
Yao Chen, Qian Li, Ting Zhao, Zhen Zhang, Guanghua Mao, Weiwei Feng, Xiangyang Wu, Liuqing Yang
Anthocyanins (ACNs) are naturally occurring components of human diet. Evidence has accumulated regarding the positive association of their intake with chronic disease. Because microbiota has been considered as a metabolic organ, the bacterial-dependent metabolisms of three types of ACNs from mulberry fruits (cyanidin-3-glucoside (C3G), cyanidin-3-rutinoside (C3R), delphinidin-3-rutinoside (D3R)) during a simulation of large intestine conditions were investigated. ACNs and metabolites were analysed and characterized by high performance liquid chromatography-electrospray ionization-mass spectrum (HPLC-ESI-MS/MS)...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28761979/probiotics-and-probiotic-metabolic-product-improved-intestinal-function-and-ameliorated-lps-induced-injury-in-rats
#6
Bo Deng, Jie Wu, Xiaohui Li, Xiaoming Men, Ziwei Xu
In the present study, we sought to determine the effects of Bacillus subtilis (BAS) and Bacillus licheniformis (BAL) in rats after lipopolysaccharide (LPS)-induced acute intestinal inflammation. We also determined whether the B. subtilis metabolic product (BASM) is as effective as the live-cell probiotic. 60 male SD rats were randomly assigned to five groups and administered a diet containing 0.05% B. licheniformis (BAL group), 0.05% B. subtilis (BAS group), 0.5% B. subtilis metabolic product (BASM group), or a basic diet (PC group and NC group) for 40 days...
July 31, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28757033/commensal-coagulase-negative-staphylococcus-from-the-udder-of-healthy-cows-inhibits-biofilm-formation-of-mastitis-related-pathogens
#7
Paula Isaac, Luciana Paola Bohl, María Laura Breser, María Soledad Orellano, Agustín Conesa, Marcela Alejandra Ferrero, Carina Porporatto
Bovine mastitis, considered the most important cause of economic losses in the dairy industry, is a major concern in veterinary medicine. Staphylococcus aureus and coagulase-negative staphylococci (CNS) are the main pathogens associated with intramammary infections, and bacterial biofilms are suspected to be responsible for the persistence of this disease. CNS from the udder are not necessarily associated with intramammary infections. In fact, some commensal CNS have been shown to have biological activities...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28757024/16s-rrna-genes-illumina-sequencing-revealed-differential-cecal-microbiome-in-specific-pathogen-free-chickens-infected-with-different-subgroup-of-avian-leukosis-viruses
#8
Xinxin Ma, Qi Wang, Hongmei Li, Chuantian Xu, Ning Cui, Xiaomin Zhao
Intestinal flora play important roles in the pathogenisis of many pathogens. This study examined the cecal microbiome of chickens infected with avian leukosis virus (ALV) using 16S rRNA genes Illumina sequencing. One-day-old specific pathogen free chicks were inoculated in the abdomen with subgroup J or K of ALV. At 21-day-old, chickens positive for ALV viremia were selected and their cecal contents were extracted and examined for the composition of gut microflora by illumina sequencing of the V3+V4 region of the 16S rRNA genes...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28754012/intra-amniotic-administration-gallus-gallus-of-cicer-arietinum-and-lens-culinaris-prebiotics-extracts-and-duck-egg-white-peptides-affects-calcium-status-and-intestinal-functionality
#9
Tao Hou, Nikolai Kolba, Raymond P Glahn, Elad Tako
Calcium (Ca) is one of the most abundant inorganic elements in the human body and has many important physiological roles. Prebiotics and bioactive peptides are two important substances used to promote calcium uptake. However, the difference in mechanisms of the calcium uptake from these two supplements is not clear. By using the Gallus gallus model and the intra-amniotic administration procedure, the aim of this study was to investigate whether Ca status, intestinal functionality, and health-promoting bacterial populations were affected by prebiotics extracted from chickpea and lentil, and duck egg white peptides (DPs)...
July 21, 2017: Nutrients
https://www.readbyqxmd.com/read/28751741/soil-microbial-community-structure-and-catabolic-activity-are-significantly-degenerated-in-successive-rotations-of-chinese-fir-plantations
#10
Zeyan Wu, Jianjuan Li, Jie Zheng, Jinfu Liu, Shuying Liu, Wenxiong Lin, Chengzhen Wu
This study examined the hypotheses that soil microbial community composition and catabolic activity would significantly degenerated by consecutive monoculture in Chinese fir plantations. The phospholipid fatty acids (PLFA) and community level physiological profiles (CLPP) methods were used to assess the variations of soil microbial community among the first rotation Chinese fir plantation (FCP), the second rotation plantation (SCP) and the third rotation plantation (TCP). The total content of PLFA biomarkers was highest in FCP, followed by SCP, and TCP was the least detected...
July 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28744281/age-related-variations-in-intestinal-microflora-of-free-range-and-caged-hens
#11
Yizhe Cui, Qiuju Wang, Shengjun Liu, Rui Sun, Yaqiang Zhou, Yue Li
Free range feeding pattern puts the chicken in a mixture of growth materials and enteric bacteria excreted by nature, while it is typically unique condition materials and enteric bacteria in commercial caged hens production. Thus, the gastrointestinal microflora in two feeding patterns could be various. However, it remains poorly understood how feeding patterns affect development and composition of layer hens' intestinal microflora. In this study, the effect of feeding patterns on the bacteria community in layer hens' gut was investigated using free range and caged feeding form...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28743928/effect-of-probiotics-on-the-meat-flavour-and-gut-microbiota-of-chicken
#12
Yan Wang, Jing Sun, Hang Zhong, Nianzhen Li, Hengyong Xu, Qing Zhu, Yiping Liu
To date, no report has demonstrated the use of beneficial microbes for contributing to the flavour characteristics and gut microbiota diversity of chicken. Here, we selected six probiotics obtained from our laboratory and supplemented them in six different combinations to 420 newborn male Qingjiaoma chickens under the same controlled living environment (60 birds, no probiotic supplements). The results showed that chicken supplemented with Bacillus species showed beneficial effects in body weight. Acetate is the major fermentation production in the chicken caecum, and chicken supplemented with Pediococcus pentosaceus had the average higher short chain fatty acids (SCFAs) contents...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28743239/acid-tolerance-properties-of-dental-biofilms-in-vivo
#13
A Senneby, J R Davies, G Svensäter, J Neilands
BACKGROUND: The ecological plaque hypothesis explains caries development as the result of the enrichment of acid tolerant bacteria in dental biofilms in response to prolonged periods of low pH. Acid production by an acid tolerant microflora causes demineralisation of tooth enamel and thus, individuals with a greater proportion of acid tolerant bacteria would be expected to be more prone to caries development. Biofilm acid tolerance could therefore be a possible biomarker for caries prediction...
July 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28737908/edible-nanoencapsulation-vehicles-for-oral-delivery-of-phytochemicals-a-perspective-paper
#14
Jie Xiao, Yong Cao, Qingrong Huang
Edible nanoencapsulation vehicles (ENVs) designed for the delivery of phytochemicals have gained increasing research interest. The major driving force for this trend is the potential bioavailability enhancement effect for phytochemicals when delivered via ENVs. ENVs affect the bioefficacy of phytochemicals by influencing their dispersion and gastrointestinal stability, rate and site of release, transportation efficiency across the endothelial layer, systemic circulation and biodistribution, and regulation of gut microflora...
August 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28737708/functional-comparison-for-lipid-metabolism-and-intestinal-and-fecal-microflora-enzyme-activities-between-low-molecular-weight-chitosan-and-chitosan-oligosaccharide-in-high-fat-diet-fed-rats
#15
Chen-Yuan Chiu, Shih-An Feng, Shing-Hwa Liu, Meng-Tsan Chiang
The present study investigated and compared the regulatory effects on the lipid-related metabolism and intestinal disaccharidase/fecal bacterial enzyme activities between low molecular weight chitosan and chitosan oligosaccharide in high-fat-diet-fed rats. Diet supplementation of low molecular weight chitosan showed greater efficiency than chitosan oligosaccharide in suppressing the increased weights in body and in liver and adipose tissues of high-fat-diet-fed rats. Supplementation of low molecular weight chitosan also showed a greater improvement than chitosan oligosaccharide in imbalance of plasma, hepatic, and fecal lipid profiles, and intestinal disaccharidase activities in high-fat-diet-fed rats...
July 24, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28735320/in-vitro-interaction-between-fumonisin-b-sub-1-sub-and-the-intestinal-microflora-of-pigs
#16
Huu Anh Dang, Attila Zsolnai, Melinda Kovacs, Istvan Bors, Andras Bonai, Brigitta Bota, Judit Szabo Fodor
The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B1 (5 μg/ml) for 0, 24 and 48 h. The plate count agar technique was applied for enumerating the amount of bacteria including aerobic, anaerobic bacteria, coliform, Escherichia coli and Lactobacillus sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, Lactobacillus, Bacteroides and Prevotella. No significant differences in the amount of bacterial groups between the experimental (buffer, chyme, and fumonisin B1) and control 1 groups (buffer + chyme) were observed in both methods...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28733207/uplc-q-tof-ms-based-urine-and-plasma-metabonomics-study-on-the-ameliorative-effects-of-aspirin-eugenol-ester-in-hyperlipidemia-rats
#17
Ning Ma, Xi-Wang Liu, Xiao-Jun Kong, Zhe Qin, Shi-Hong Li, Zeng-Hua Jiao, Peng-Cheng Dong, Ya-Jun Yang, Jian-Yong Li
The main objective of this study was to investigate the ameliorative effects of aspirin eugenol ester (AEE) in hyperlipidemic rat. After five-week oral administration of AEE in high fat diet (HFD)-induced hyperlipidemic rats, the impact of AEE on plasma and urine metabonomics was investigated to explore the underlying mechanism by UPLC-Q-TOF/MS analysis. Blood lipid levels and histopathological changes of liver, stomach and duodenum were also evaluated after AEE treatment. Without obvious gastrointestinal (GI) side effects, AEE significantly relieved fatty degeneration of liver and reduced triglyceride (TG), low density lipoprotein (LDL) and total cholesterol (TCH) (P<0...
July 18, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28732111/characterization-of-metabolic-pathways-and-absorption-of-sea-cucumber-saponins-holothurin-a-and-echinoside-a-in-vitro-and-in-vivo
#18
Shanshan Song, Lingyu Zhang, Jian Cao, Gao Xiang, Peixu Cong, Ping Dong, Zhaojie Li, Changhu Xue, Yong Xue, Yuming Wang
Sea cucumber saponins (SCSs) exhibit a wide spectrum of bioactivities, but their metabolic characteristics are not well elucidated. In this study, the metabolism of holothurin A (HA) and echinoside A (EA), 2 major saponins in sea cucumber, by gut microflora were investigated. First, we conducted an in vitro study, where in the SCSs were incubated with intestinal microflora and the metabolites were detected by high pressure liquid chromatography-high resolution mass spectrometry. We also conducted an in vivo study on rats, where in the intestinal contents, serum, urine, and feces were collected and evaluated after oral administration of SCSs...
July 21, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28730858/management-of-children-with-persistent-group-a-streptococcal-carriage
#19
Maria E Zacharioudaki, Emmanouil Galanakis
Chronic GAS carrier state is best defined as the prolonged presence of group A β-haemolytic Streptococcus (GAS) in the pharynx without evidence of infection or inflammation. Chronic GAS carriers have a low risk of immune mediated complications. Persistent pharyngeal carriage often raises management issues. In this study, we review the evidence on the management of persistent GAS carriage in children and propose a management algorithm. Areas covered: Chronic GAS pharyngeal carriage is quite common affecting 10-20% of school-aged children...
July 31, 2017: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/28728393/effects-of-dietary-supplementation-of-detoxified-rhus-verniciflua-sap-on-egg-production-yolk-lipid-and-intestinal-microflora-in-laying-hens
#20
Byoung-Ki An, Je-Hun Kim, Lan Zheng, Byung-Hern Moon, Kyung-Woo Lee
Objective: This study was conducted to investigate the effects of dietary detoxified Rhus verniciflua sap (RVS) on production performance, egg quality, lipid fractions of egg yolk, liver and serum, and the profile of cecal microflora in laying hens. Methods: Two hundred 52-week-old Hy-Line Brown layers were randomly divided into 4 groups with 5 replicates per group (2 hens per cage, 5 cages per replicate) and were provided with one of 4 experimental diets containing 0, 0...
May 14, 2017: Asian-Australasian Journal of Animal Sciences
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