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https://www.readbyqxmd.com/read/28720421/assessment-of-the-hiv-1-reservoir-in-cd4-regulatory-t-cells-by-a-droplet-digital-pcr-based-approach
#1
Gábor Artúr Dunay, Anastasia Solomatina, Silke Kummer, Anja Hüfner, Julia Katharina Bialek, Johanna Maria Eberhard, Eva Tolosa, Joachim Hauber, Julian Schulze Zur Wiesch
The relative contribution of regulatory T cells (Treg) as reservoir of HIV-1 in patients on chronic antiretroviral therapy is unclear to date. The aim of the current study was to assess the total HIV DNA burden and replication competent viral reservoir in Treg in comparison to central and effector memory cells (Tcm and Tem). Peripheral blood mononuclear cells were obtained from 10 HIV patients treated with antiretroviral therapy and one HIV elite controller. Droplet Digital PCR (ddPCR) was used to quantify total HIV DNA loads in FACS sorted CD4+ Treg (CD25+CD127lo) as compared with Tcm (CD45RO+CCR7+) and Tem (CD45RO+CCR7-)...
July 15, 2017: Virus Research
https://www.readbyqxmd.com/read/28714759/effects-of-rigid-and-kinesio-taping-on-shoulder-rotation-motions-posterior-shoulder-tightness-and-posture-in-asymptomatic-overhead-athletes-a-randomized-controlled-trial
#2
Damla Gulpinar, Sibel Tekeli Ozer, Sevgi S Yesilyaprak
CONTEXT: Alterations in posture and motion patterns are thought to play a role in developing shoulder injuries in overhead athletes. Taping is widely used in the sporting population, but there are limited empirical data regarding its effectiveness. OBJECTIVES: To determine and compare the effects of rigid and kinesio taping on shoulder rotation motions, posterior shoulder tightness (PST) and posture in overhead athletes. DESIGN: Randomized controlled trial...
July 17, 2017: Journal of Sport Rehabilitation
https://www.readbyqxmd.com/read/28713458/trunk-strength-characteristics-of-elite-alpine-skiers-a-comparison-with-physically-active-controls
#3
Carolin Hildebrandt, Lisa Müller, Christian Heisse, Christian Raschner
Core muscle imbalances and weak trunk strength are relevant for injury prevention and performance. Information regarding core strength requirements and ideal flexion/extension ratios in alpine skiing is limited. We aimed to compare trunk strength capacities in elite alpine skiers with those of a matched control group. The concentric maximal trunk flexion and extension of 109 elite skiers and 47 active controls were measured at 150°/s in a ballistic mode using the CON-TREX® TP 1000 test system. The relative flexion peak torque was higher in male ski racers (p = 0...
June 2017: Journal of Human Kinetics
https://www.readbyqxmd.com/read/28704881/cardiovascular-and-autonomic-responses-to-a-maximal-exercise-test-in-elite-youngsters
#4
Cristina Blasco-Lafarga, Borja Camarena, Manuel Mateo-March
To analyze cardiovascular and autonomic responses in elite youngsters, 13 male cyclists (15.43±0.51 years) performed a graded-test until voluntary exhaustion. Oxygen consumption (VO2), blood lactate (BLa), arterial oxygen saturation (SaO2), respiratory exchange ratio (RER) and rating of perceived exertion (RPE) were collected, while heart rate (HR) was registered for heart rate variability (HRV) analyses, looking for linear and nonlinear comparisons. Cyclists reached maximal exertion [RPE: 19.14±0.94; BLa: 8...
July 13, 2017: International Journal of Sports Medicine
https://www.readbyqxmd.com/read/28701393/hiv-1-env-and-vpu-specific-antibody-dependent-cellular-cytotoxicity-responses-associated-with-elite-control-of-hiv
#5
Vijaya Madhavi, Bruce D Wines, Janaki Amin, Sean Emery, Ester Lopez, Anthony Kelleher, Rob J Center, P Mark Hogarth, Amy W Chung, Stephen J Kent, Ivan Stratov
Studying HIV-infected individuals who control HIV replication (elite controllers, ECs) enable exploration of effective anti-HIV immunity. HIV Env- and non-Env-specific antibody-dependent cellular cytotoxicity (ADCC) may contribute to protection from progressive HIV infection but the evidence is limited. We recruited 22 ECs and matched them with 44 viremic subjects. HIV Env- and Vpu-specific ADCC responses were studied in sera using a novel ELISA-based dimeric recombinant soluble (rs) FcγRIIIa-binding assay, surface plasmon resonance, antibody-dependent natural killer (NK) cell activation assays and ADCC-mediated killing assays...
July 12, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28695289/the-role-of-mhc-class-i-gene-products-in-siv-infection-of-macaques
#6
REVIEW
Zachary A Silver, David I Watkins
Human immunodeficiency virus (HIV) remains among the most significant public health threats worldwide. Despite three decades of research following the discovery of HIV, a preventive vaccine remains elusive. The study of HIV elite controllers has been crucial to elaborate the genetic and immunologic determinants that underlie control of HIV replication. Coordinated studies of elite control in humans have, however, been limited by variability among infecting viral strains, host genotype, and the uncertainty of the timing and route of infection...
July 10, 2017: Immunogenetics
https://www.readbyqxmd.com/read/28695193/testing-for-voter-rigging-in-small-polling-stations
#7
Raúl Jimenez, Manuel Hidalgo, Peter Klimek
Nowadays, a large number of countries combine formal democratic institutions with authoritarian practices. Although in these countries the ruling elites may receive considerable voter support, they often use several manipulation tools to control election outcomes. A common practice of these regimes is the coercion and mobilization of large numbers of voters. This electoral irregularity is known as voter rigging, distinguishing it from vote rigging, which involves ballot stuffing or stealing. We develop a statistical test to quantify the extent to which the results of a particular election display traces of voter rigging...
June 2017: Science Advances
https://www.readbyqxmd.com/read/28693882/bone-density-and-cross-sectional-geometry-of-the-proximal-femur-are-bilaterally-elevated-in-elite-cricket-fast-bowlers
#8
Matthew J Lees, Clive B Beggs, Matthew J Barlow, Zoe H Rutherford, Kunwar Bansil, Lisa Gannon, Karen Hind
The skeleton of a cricket fast bowler is exposed to a unique combination of gravitational and torsional loading in the form of substantial ground reaction forces delivered through the front landing foot, and anterior-posterior shear forces mediated by regional muscle contractions across the lumbo-pelvic region. The objectives of this study were to compare the hip structural characteristics of elite fast bowlers with recreationally active age-matched controls, and to examine unilateral bone properties in fast bowlers...
July 7, 2017: Journal of Clinical Densitometry
https://www.readbyqxmd.com/read/28686676/exotic-qtl-improve-grain-quality-in-the-tri-parental-wheat-population-sw84
#9
Ioanna-Pavlina Nedelkou, Andreas Maurer, Anne Schubert, Jens Léon, Klaus Pillen
DEVELOPING THE TRI-PARENTAL EXOTIC WHEAT POPULATION SW84: Genetic diversity of cultivated wheat was markedly reduced, first, during domestication and, second, since the onset of modern elite breeding. There is an increasing demand for utilizing genetic resources to increase genetic diversity and, simultaneously, to improve agronomic performance of cultivated wheat. To locate favorable effects of exotic wheat alleles, we developed the tri-parental wheat population SW84. The population was derived from crossing the hexaploid spring wheat cultivars Triso and Devon with one synthetic exotic donor accession, Syn084L, followed by two rounds of backcrossing and three rounds of selfing...
2017: PloS One
https://www.readbyqxmd.com/read/28683184/comparison-of-ultra-high-performance-supercritical-fluid-chromatography-ultra-high-performance-liquid-chromatography-and-gas-chromatography-for-the-separation-of-synthetic-cathinones
#10
Stephanie Carnes, Stacey O'Brien, Angelica Szewczak, Lauriane Tremeau-Cayel, Walter F Rowe, Bruce McCord, Ira S Lurie
A comparison of ultra high performance supercritical fluid chromatography, ultra high performance liquid chromatography, and gas chromatography for the separation of synthetic cathinones has been conducted. Nine different mixtures of bath salts were analyzed in this study. The three different chromatographic techniques were examined using a general set of controlled synthetic cathinones as well as a variety of other synthetic cathinones that exist as positional isomers. Overall 35 different synthetic cathinones were analyzed...
July 6, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28677944/heart-rate-response-and-recovery-in-wheelchair-tetraplegic-rugby-athletes-a-pilot-study
#11
Michelle C Coutinho, Patrícia S Vigário, Agnaldo J Lopes, Fernando S Guimarães
BACKGROUND: Subjects with spinal cord injuries (SCI) have impaired autonomic cardiac regulation and increased risk of cardiovascular disease. In face of the insufficient research data on the autonomic control of physically active subjects with SCI, this study aimed at describing the chronotropic response, as well as the heart rate recovery behaviour of a cohort of elite wheelchair rugby athletes. METHODS: This is a cross-sectional study including a convenience sample of 17 elite wheelchair rugby athletes with tetraplegia...
July 5, 2017: Journal of Sports Medicine and Physical Fitness
https://www.readbyqxmd.com/read/28677873/editorial-the-politics-of-reproduction-and-parenting-cultures-procreation-pregnancy-childbirth-and-childrearing
#12
EDITORIAL
Joanna Latimer, Gareth M Thomas
'Controlling life was and is to be achieved in part by rationalizing and industrializing reproductive processes. Multiple heterogeneous and contradictory groups have had an interest in achieving such control - from elites seeking to control others to individuals, especially women, trying to get a grip on their own lives through controlling their reproduction; from eugenicists ultimately trying to control evolution to neo-Malthusians trying to control national and population size; from philanthropists and foundation executives trying to shape the future of science and human life in varied directions to reproductive scientists trying to do their research … The biomedicalization of life itself (human, plant, and animal) is the key overarching and usually taken for granted social process here' (Clarke : 273-5)...
July 2017: Sociology of Health & Illness
https://www.readbyqxmd.com/read/28676808/gibberellins-promote-brassinosteroids-action-and-both-increase-heterosis-for-plant-height-in-maize-zea-mays-l
#13
Songlin Hu, Cuiling Wang, Darlene L Sanchez, Alexander E Lipka, Peng Liu, Yanhai Yin, Michael Blanco, Thomas Lübberstedt
Brassinosteroids (BRs) and Gibberellins (GAs) are two classes of plant hormones affecting plant height (PHT). Thus, manipulation of BR and GA levels or signaling enables optimization of crop grain and biomass yields. We established backcross (BC) families, selected for increased PHT, in two elite maize inbred backgrounds. Various exotic accessions used in the germplasm enhancement in maize project served as donors. BC1-derived doubled haploid lines in the same two elite maize inbred backgrounds established without selection for plant height were included for comparison...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28674509/acceptance-and-commitment-therapy-for-the-treatment-of-music-performance-anxiety-a-pilot-study-with-student-vocalists
#14
David G Juncos, Glenn A Heinrichs, Philip Towle, Kiera Duffy, Sebastian M Grand, Matthew C Morgan, Jonathan D Smith, Evan Kalkus
This study investigated the use of Acceptance and Commitment Therapy (ACT) as a treatment for music performance anxiety (MPA) in an uncontrolled pilot design. ACT is a newer, "third-wave" therapy that differs from previous MPA treatments, because its goal is not to reduce symptoms of MPA. Rather, ACT aims to enhance psychological flexibility in the presence of unwanted symptoms through the promotion of six core processes collectively known as the ACT "Hexaflex." A small group of student vocalists (N = 7) from an elite choral college were recruited using objective criteria for evaluating MPA...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28673119/biomechanical-differences-of-arm-swing-countermovement-jumps-on-sand-and-rigid-surface-performed-by-elite-beach-volleyball-players
#15
George Giatsis, Vassilios Panoutsakopoulos, Iraklis A Kollias
The purpose of this study was to investigate the possible arm swing effect on the biomechanical parameters of vertical counter movement jump due to differences of the compliance of the take-off surface. Fifteen elite male beach-volleyball players (26.2 ± 5.9 years; 1.87 ± 0.05 m; 83.4 ± 6.0 kg; mean ± standard deviation, respectively) performed counter movement jumps on sand and on a rigid surface with and without an arm swing. Results showed significant (p < .05) surface effects on the jump height, the ankle joint angle at the lowest height of the body center of mass and the ankle angular velocity...
July 4, 2017: Journal of Sports Sciences
https://www.readbyqxmd.com/read/28672745/hiv-avidity-index-performance-using-a-modified-fourth-generation-immunoassay-to-detect-recent-hiv-infections
#16
Barbara Suligoi, Vincenza Regine, Mariangela Raimondo, Anna Rodella, Luigina Terlenghi, Arnaldo Caruso, Patrizia Bagnarelli, Maria Rosaria Capobianchi, Nadia Zanchetta, Valeria Ghisetti, Claudio Galli
BACKGROUND: Detecting recent HIV infections is important to evaluate incidence and monitor epidemic trends. We aimed to evaluate the diagnostic performance and accuracy of the avidity index (AI) for discriminating for recent HIV infections. METHODS: We collected serum samples from HIV-1 positive individuals: A) with known date of infection (midpoint in time between last HIV-negative and first HIV-positive test); B) infected for >1 year. Samples were divided into two aliquots: one diluted with phosphate buffered saline (PBS) and the other with 1 M guanidine...
June 24, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28671952/differences-in-serum-iga-responses-to-hiv-1-gp41-in-elite-controllers-compared-to-viral-suppressors-on-highly-active-antiretroviral-therapy
#17
Rafiq Nabi, Zina Moldoveanu, Qing Wei, Elizabeth T Golub, Helen G Durkin, Ruth M Greenblatt, Betsy C Herold, Marek J Nowicki, Seble Kassaye, Michael W Cho, Abraham Pinter, Alan L Landay, Jiri Mestecky, Pamela A Kozlowski
Mechanisms responsible for natural control of human immunodeficiency type 1 (HIV) replication in elite controllers (EC) remain incompletely defined. To determine if EC generate high quality HIV-specific IgA responses, we used Western blotting to compare the specificities and frequencies of IgA to HIV antigens in serum of gender-, age- and race-matched EC and aviremic controllers (HC) and viremic noncontrollers (HN) on highly active antiretroviral therapy (HAART). Concentrations and avidity of IgA to HIV antigens were measured using ELISA or multiplex assays...
2017: PloS One
https://www.readbyqxmd.com/read/28670285/muscle-and-tendon-adaptation-in-adolescence-elite-volleyball-athletes-compared-to-untrained-boys-and-girls
#18
Falk Mersmann, Georgios Charcharis, Sebastian Bohm, Adamantios Arampatzis
Though the plasticity of human tendons is well explored in adults, it is still unknown how superimposed mechanical loading by means of athletic training affects the properties of tendons during maturation. Due to the increased responsiveness of muscle to mechanical loading, adolescence is an important phase to investigate the effects of training on the mechanical properties of tendons. Hence, in the present study we compared vastus lateralis (VL) architecture, muscle strength of the knee extensor muscles and patellar tendon mechanical properties of male and female adolescent elite athletes to untrained boys and girls...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28665447/effect-of-artificial-saliva-with-different-ph-levels-on-the-cytotoxicity-of-soft-denture-lining-materials
#19
Canan Akay, Merve Ç Tanış, Handan Sevim
BACKGROUND: The aim of this study was to evaluate the cytotoxic effects of 9 different soft denture liners on the viability of L-929 mouse fibroblast cells at different incubation periods by storing them in artificial saliva (AS) with different pH levels. METHODS: 96 disk samples from each lining material were prepared and divided into 4 groups: GI: No treatment; GII: Stored in artificial saliva with pH 3 for 21 days; Group III: Stored in artificial saliva with pH 7 for 21 days; and Group IV: Stored in artificial saliva with pH 14 for 21 days...
June 27, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28662531/effects-of-in-season-explosive-strength-training-on-maximal-leg-strength-jumping-sprinting-and%C3%A2-intermittent-aerobic-performance-in-male-handball-athletes
#20
Souhail Hermassi, Mohamed Souhaiel Chelly, Georg Fieseler, Thomas Bartels, Stephan Schulze, Karl-Stefan Delank, Roy J Shephard, René Schwesig
Background Team handball is an intense ball sport with specific requirements on technical skills, tactical understanding, and physical performance. The ability of handball players to develop explosive efforts (e. g. sprinting, jumping, changing direction) is crucial to success. Objective The purpose of this pilot study was to examine the effects of an in-season high-intensity strength training program on the physical performance of elite handball players. Materials and methods Twenty-two handball players (a single national-level Tunisian team) were randomly assigned to a control group (CG; n = 10) or a training group (TG; n = 12)...
June 29, 2017: Sportverletzung Sportschaden: Organ der Gesellschaft Für Orthopädisch-Traumatologische Sportmedizin
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