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Genotoxicity of Fishes

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https://www.readbyqxmd.com/read/28890382/in-vitro-genotoxicity-and-in-vivo-subchronic-evaluation-of-the-anti-inflammatory-pyrazole-compound-lqfm021
#1
Soraia Santa de Moura, Renato Ivan de Ávila, Lara Barroso Brito, Rhaul de Oliveira, Gisele Augusto Rodrigues de Oliveira, Francine Pazini, Ricardo Menegatti, Aline Carvalho Batista, Cesar Koppe Grisolia, Marize Campos Valadares
Scientific evidences have highlighted 5-(1-(3-fluorophenyl)-1H-pyrazol-4-yl)-2H-tetrazole (LQFM021) as a promising anti-inflammatory, analgesic and antinociceptive agent due to its effects on peripheral opioid receptors associated with activation of the nitric oxide/cGMP/KATP pathway. Despite these important pharmacological findings, toxicity data of LQFM021 are scarce. Thus, this study investigated the in vitro genotoxicity of LQFM021 through cytokinesis-block micronucleus assay (OECD Nº 487/2014). Moreover, zebrafish model was used to assess the embryotoxicity potential of LQFM021 using fish embryo toxicity test (OECD Nº 236/2013) with extended exposure to evaluate subchronic larval development...
September 7, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28868735/micronuclei-and-other-erythrocyte-nuclear-abnormalities-in-fishes-from-the-great-lakes-basin-usa
#2
Ryan P Braham, Vicki S Blazer, Cassidy H Shaw, Patricia M Mazik
Biological markers (biomarkers) sensitive to genotoxic and mutagenic contamination in fishes are widely used to identify exposure effects in aquatic environments. The micronucleus assay was incorporated into a suite of indicators to assess exposure to genotoxic and mutagenic contamination at five Great Lakes Areas of Concern (AOCs), as well as one non-AOC (reference) site. The assay allowed enumeration of micronuclei as well as other nuclear abnormalities for both site and species comparisons. Erythrocyte abnormality data was also compared to skin and liver tumor prevalence and hepatic transcript abundance...
September 4, 2017: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/28844240/developmental-toxicity-and-dna-damaging-properties-of-silver-nanoparticles-in-the-catfish-clarias-gariepinus
#3
Alaa El-Din H Sayed, Hamdy A M Soliman
Although, silver nanoparticles (AgNPs) are used in many different products, little information is known about their toxicity in tropical fish embryos. Therefore, this study evaluated the developmental toxicity of waterborne silver nanoparticles in embryos of Clarias gariepinus. Embryos were treated with (0, 25, 50, 75ng/L silver nanoparticles) in water up to 144h postfertilization stage (PFS). Results revealed various morphological malformations including notochord curvature and edema. The mortality rate, malformations, and DNA fragmentation in embryos exposed to silver nanoparticles increased in a dose- and embryonic stage-dependent manner...
October 2017: Mutation Research
https://www.readbyqxmd.com/read/28835124/in-vitro-and-in-vivo-characteristics-of-biogenic-high-surface-silica-nanoparticles-in-a549-lung-cancer-cell-lines-and-danio-rerio-model-systems-for-inorganic-biomaterials-development
#4
Suriyaprabha Rangaraj, Rajendran Venkatachalam
Silica based organic-inorganic hybrids are turned over the most necessitate biomaterial due to their exotic biomedical properties. Colloidal silica nanoparticles (SNPs) of high surface area are synthesized from the bamboo wastes (leave biomass) as a viable and promising alternative to synthetic silica sol through alkaline extraction process. Physico-chemical properties of the prepared silica powders are examined employing extensive characterization tools. The characteristic results of the silica sol demonstrate amorphous particles (average size: 25 nm) with relatively high surface area (428 m(2) g(-1)) and spherical morphology...
August 24, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28819745/bioassessment-of-the-effluents-discharged-from-two-export-oriented-industrial-zones-located-in-kelani-river-basin-sri-lanka-using-erythrocytic-responses-of-the-fish-nile-tilapia-oreochromis-niloticus
#5
C K Hemachandra, A Pathiratne
Complex effluents originating from diverse industrial processes in industrial zones could pose cytotoxic/genotoxic hazards to biota in the receiving ecosystems which cannot be revealed by conventional monitoring methods. This study assessed potential cytotoxicity/genotoxicity of treated effluents of two industrial zones which are discharged into Kelani river, Sri Lanka combining erythrocytic abnormality tests and comet assay of the tropical model fish, Nile tilapia. Exposure of fish to the effluents induced erythrocytic DNA damage and deformed erythrocytes with serrated membranes, vacuolations, nuclear buds and micronuclei showing cytotoxic/genotoxic hazards in all cases...
August 17, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28792275/subacute-toxicity-effects-of-deltamethrin-on-oxidative-stress-markers-in-rainbow-trout
#6
Antonia Concetta Elia, Federica Giorda, Nicole Pacini, Ambrosius Josef Martin Dörr, Tommaso Scanzio, Marino Prearo
Deltamethrin is a pyrethroid insecticide that is considered extremely toxic to aquatic organisms. We evaluated the effect of subacute doses (0.033, 0.1, or 0.3 mg/kg) of deltamethrin on micronucleus frequency and oxidative stress markers in Rainbow Trout Oncorhynchus mykiss at 1, 2, 3, 4, and 8 d after intracoelomic injection. No genotoxic damage was recorded, as no specimen showed a micronucleus number above the physiological range. Deltamethrin exposure elicited a transient reduction in the levels of superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase and a persistent decrease in glutathione reductase activity...
September 2017: Journal of Aquatic Animal Health
https://www.readbyqxmd.com/read/28756254/evaluation-of-chlorpyrifos-effects-alone-and-combined-with-lipopolysaccharide-stress-on-dna-integrity-and-immune-responses-of-the-three-spined-stickleback-gasterosteus-aculeatus
#7
Adrien Marchand, Jean-Marc Porcher, Cyril Turies, Edith Chadili, Olivier Palluel, Patrick Baudoin, Stéphane Betoulle, Anne Bado-Nilles
Organism immune defences might be weakened by pollutants, largely detected in aquatic ecosystems, leading to the facilitation for opportunistic pathogens to infect organisms. In this context, destabilization of fish non-specific immune parameters and erythrocyte DNA integrity was tested, on a model fish species, the three-spined stickleback (Gasterosteus aculeatus), after exposure to chlorpyrifos (CPF). Alone, pesticide exposure induced a genotoxic potential (chlorpyrifos at 1.75 and 0.88µg/L) in addition to a decrease in phagocytosis capacity and a stimulation of respiratory burst...
July 27, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28735742/genotoxicity-of-gemfibrozil-in-the-gilthead-seabream-sparus-aurata
#8
A Barreto, L G Luis, A M V M Soares, P Paíga, L H M L M Santos, C Delerue-Matos, K Hylland, S Loureiro, M Oliveira
Widespread use of pharmaceuticals and suboptimal wastewater treatment have led to increased levels of these substances in aquatic ecosystems. Lipid-lowering drugs such as gemfibrozil, which are among the most abundant human pharmaceuticals in the environment, may have deleterious effects on aquatic organisms. We examined the genotoxicity of gemfibrozil in a fish species, the gilthead seabream (Sparus aurata), which is commercially important in southern Europe. Following 96-h waterborne exposure, molecular (erythrocyte DNA strand breaks) and cytogenetic (micronuclei and other nuclear abnormalities in cells) endpoints were measured...
September 2017: Mutation Research
https://www.readbyqxmd.com/read/28672228/uranium-mining-wastes-the-use-of-the-fish-embryo-acute-toxicity-test-fet-test-to-evaluate-toxicity-and-risk-of-environmental-discharge
#9
J Lourenço, S Marques, F P Carvalho, J Oliveira, M Malta, M Santos, F Gonçalves, R Pereira, S Mendo
Active and abandoned uranium mining sites often create environmentally problematic situations, since they cause the contamination of all environmental matrices (air, soil and water) with stable metals and radionuclides. Due to their cytotoxic, genotoxic and teratogenic properties, the exposure to these contaminants may cause several harmful effects in living organisms. The Fish Embryo Acute Toxicity Test (FET) test was employed to evaluate the genotoxic and teratogenic potential of mine liquid effluents and sludge elutriates from a deactivated uranium mine...
December 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28669044/genotoxicity-and-cytotoxicity-response-to-environmentally-relevant-complex-metal-mixture-zn-cu-ni-cr-pb-cd-accumulated-in-atlantic-salmon-salmo-salar-part-i-importance-of-exposure-time-and-tissue-dependence
#10
Milda Stankevičiūtė, Gintarė Sauliutė, Gintaras Svecevičius, Nijolė Kazlauskienė, Janina Baršienė
Health impact of metal mixture at environment realistic concentrations are difficult to predict especially for long-term effects where cause-and-effect relationships may not be directly obvious. This study was aimed to evaluate metal mixture (Zn-0.1, Cu-0.01, Ni-0.01, Cr-0.01, Pb-0.005 and Cd-0.005 mg/L, respectively for 1, 2, 4, 7, 14 and 28 days at concentrations accepted for the inland waters in EU) genotoxicity (micronuclei, nuclear buds, nuclear buds on filament), cytotoxicity (8-shaped nuclei, fragmented-apoptotic erythrocytes), bioaccumulation, steady-state and the reference level of geno-cytotoxicity in hatchery-reared Atlantic salmon tissues...
July 1, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28655460/bioaccumulation-of-metals-and-biomarkers-of-environmental-stress-in-parablennius-sanguinolentus-pallas-1814-sampled-along-the-italian-coast
#11
Margherita Ferrante, Anna Maria Pappalardo, Venera Ferrito, Valentina Pulvirenti, Carmelo Fruciano, Alfina Grasso, Salvatore Sciacca, Concetta Tigano, Chiara Copat
Heavy metal pollution is one of the greatest threats to the ecosystems because it degrades the habitat and is potentially toxic to wildlife and human populations. In the last few decades, bioaccumulation studies performed with a multimarker approach have been a valuable tool for the investigation of environmental and animal safety. We perform an analysis of a benthic teleost fish species - Parablennius sanguinolentus - sampled at several Italian coastal sites with different degrees of anthropogenic pressure...
June 24, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28641238/water-soluble-fraction-of-petroleum-induces-genotoxicity-and-morphological-effects-in-fat-snook-centropomus-parallelus
#12
Laila Carine Campos Medeiros, Frederico Augusto Cariello Delunardo, Larissa Novaes Simões, Marcelo Gustavo Paulino, Thais Souza Vargas, Marisa Narciso Fernandes, Rodrigo Scherer, Adriana Regina Chippari-Gomes
Petroleum hydrocarbons are one of the primary organic chemicals found in water bodies, and the water-soluble fraction of petroleum (WSFP) may be responsible for much of the toxic effects. In the present study, genotoxicity assays and histopathological analysis of the gills were analyzed for two experimental protocols: 1) Juvenile Centropomus parallelus were exposed to different concentrations of WFSP (0%, 25%, 50% and 75%) for 96h; 2) A second fish group was exposed to 50% WFSP for 168h followed by a post-exposure period for 168h in clean water (recovery)...
June 19, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28622554/evaluation-of-dna-damage-induced-by-gamma-radiation-in-gill-and-muscle-tissues-of-cyprinus-carpio-and-their-relative-sensitivity
#13
Praveen Kumar M K, Soorambail K Shyama, Avelyno D'Costa, Samit B Kadam, Bhagatsingh Harisingh Sonaye, Ramesh Chandra Chaubey
The effect of radiation on the aquatic environment is of major concern in recent years. Limited data is available on the genotoxicity of gamma radiation on different tissues of aquatic organisms. Hence, the present investigation was carried out to study the DNA damage induced by gamma radiation in the gill and muscle tissues and their relative sensitivity using the comet assay in the freshwater teleost fish, common carp (Cyprinus carpio). The comet assay was optimized and validated in common carp using cyclophosphamide (CP), a reference genotoxic agent...
June 13, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28601519/cytotoxic-and-genotoxic-affects-of-acid-mine-drainage-on-fish-channa-punctata-bloch
#14
B Talukdar, H K Kalita, S Basumatary, D J Saikia, D Sarma
The investigation deals with the effects of Acid Mine Drainage (AMD) of coal mine on fish Channa punctata (Bloch) by examining the incidence of haematological, morphological, histological changes and DNA fragmentation in tissues of C. punctata in laboratory condition. For this study fishes were exposed to 10% of AMD for a period of 30 days. The fusion of the primary and secondary gill lamellae, distortion, loss of alignment, deposition of worn out tissues and mucous on the surface of the lamella in the gills; degeneration of morphological architecture, loss of alignment of tubules, mucous deposition in the kidney; cellular damage, cellular necrosis, extraneous deposition on the surface, pore formation in the liver are some important changes detected by scanning electron microscopy...
June 8, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28583304/transgenerational-inheritance-of-neurobehavioral-and-physiological-deficits-from-developmental-exposure-to-benzo-a-pyrene-in-zebrafish
#15
Andrea L Knecht, Lisa Truong, Skylar W Marvel, David M Reif, Abraham Garcia, Catherine Lu, Michael T Simonich, Justin G Teeguarden, Robert L Tanguay
Benzo[a]pyrene (B[a]P) is a well-known genotoxic polycylic aromatic compound whose toxicity is dependent on signaling via the aryl hydrocarbon receptor (AHR). It is unclear to what extent detrimental effects of B[a]P exposures might impact future generations and whether transgenerational effects might be AHR-dependent. This study examined the effects of developmental B[a]P exposure on 3 generations of zebrafish. Zebrafish embryos were exposed from 6 to 120h post fertilization (hpf) to 5 and 10μM B[a]P and raised in chemical-free water until adulthood (F0)...
August 15, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28575799/application-of-fish-cell-lines-for-evaluating-the-chromium-induced-cytotoxicity-genotoxicity-and-oxidative-stress
#16
G Taju, S Abdul Majeed, K S N Nambi, A S Sahul Hameed
In the present study, we hypothesize that cytotoxicity, genotoxicity and oxidative stress play a key role in chromium induced toxicity in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines after 24 h exposure. Three fish species namely Lates calcarifer, Etroplus suratensis and Catla catla were exposed to the concentrations of 0, 10, 20, 30, 40 and 50 mg/L of chromium for 96 h under static conditions for conducting acute toxicity tests. LC50 was then calculated. The percentage cell survival was assessed by multiple endpoints such as MTT, NR, AB and CB assays in the seven fish cell lines exposed to different concentrations of chromium and EC50 values of all the four endpoints were calculated...
October 2017: Chemosphere
https://www.readbyqxmd.com/read/28573396/toxicity-assessment-of-magnetosomes-in-different-models
#17
T Revathy, M A Jayasri, K Suthindhiran
Magnetosomes are nanosized iron oxide particles surrounded by lipid membrane synthesized by magnetotactic bacteria (MTB). Magnetosomes have been exploited for a broad range of biomedical and biotechnological applications. Due to their enormous potential in the biomedical field, its safety assessment is necessary. Detailed research on the toxicity of the magnetosomes was not studied so far. This study focuses on the toxicity assessment of magnetosomes in various models such as Human RBC's, WBC's, mouse macrophage cell line (J774), Onion root tip and fish (Oreochromis mossambicus)...
June 2017: 3 Biotech
https://www.readbyqxmd.com/read/28554110/evaluation-of-the-detoxification-efficiencies-of-coking-wastewater-treated-by-combined-anaerobic-anoxic-oxic-a-2-o-and-advanced-oxidation-process
#18
Chunhong Na, Ying Zhang, Xie Quan, Shuo Chen, Wei Liu, Yaobin Zhang
Coking wastewater contains many types of toxic and hazardous pollutants that have serious toxic effects on human beings as well as aquatic organisms. However, few studies have evaluated the detoxification efficiencies of the treatment processes that are extensively performed in operational coking wastewater treatment plants (WWTPs). This study investigates the detoxification efficiencies of a combined anaerobic-anoxic-oxic (A(2)O)-ozonation and A(2)O-Fenton oxidation process in two coking WWTPs using an acute immobilization test for Daphnia magna, acute toxicity test for adult zebrafish, embryo toxicity test for zebrafish and the comet assay...
May 22, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28551207/genotoxic-and-mutagenic-assessment-of-iron-oxide-maghemite-%C3%AE-fe2o3-nanoparticle-in-the-guppy-poecilia-reticulata
#19
Gabriel Qualhato, Thiago Lopes Rocha, Emília Celma de Oliveira Lima, Daniela Melo E Silva, Júlio Roquete Cardoso, Cesar Koppe Grisolia, Simone Maria Teixeira de Sabóia-Morais
The environmental risk of nanomaterials (NMs) designed and used in nanoremediation process is of emerging concern, but their ecotoxic effects to aquatic organism remains unclear. In this study, the citrate-coated (maghemite) nanoparticles (IONPs) were synthesized and its genotoxic and mutagenic effects were investigated in the female guppy Poecilia reticulata. Fish were exposed to IONPs at environmentally relevant iron concentration (0.3 mg L(-1)) during 21 days and the animals were collected at the beginning of the experiment and after 3, 7, 14 and 21 days of exposure...
May 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28545014/paracetamol-causes-endocrine-disruption-and-hepatotoxicity-in-male-fish-rhamdia-quelen-after-subchronic-exposure
#20
Izonete Cristina Guiloski, João Luiz Coelho Ribas, Laercio Dante Stein Piancini, Ana Carolina Dagostim, Silvana Maris Cirio, Luis Fernando Fávaro, Suelen Lúcio Boschen, Marta Margarete Cestari, Cláudio da Cunha, Helena Cristina Silva de Assis
Paracetamol is one of the most widely sold non-prescription drugs. This study aimed to evaluate the effects of the paracetamol on reproductive, biochemical, genetic, histopathological and hematogical biomarkers by waterborne exposure. Male fish of Rhamdia quelen were exposed to environmental concentrations of paracetamol (0, 0.25, 2.5μg/L) in a semi-static bioassay for 21days. Hemoglobin and hematocrit were reduced upon exposure to 0.25μg/L of paracetamol. Leukocytes and thrombocytes increased after paracetamol exposure...
July 2017: Environmental Toxicology and Pharmacology
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