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Genotoxicity of Fishes

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https://www.readbyqxmd.com/read/28511133/phorate-induced-oxidative-stress-dna-damage-and-differential-expression-of-p53-apaf-1-and-cat-genes-in-fish-channa-punctatus-bloch-1793
#1
Arun Ratn, Yashika Awasthi, Manoj Kumar, Sunil Kumar Singh, Renu Tripathi, Sunil P Trivedi
The present study was conducted to assess the in-vivo activities of certain molecular biomarkers under the impact of phorate exposure. Fish, Channa punctatus (35 ± 3.0 g; 14.5 ± 1.0 cm; Actinopterygii) were subjected to semi-static conditions having 5% (0.0375 mg/L for T1 group) and 10% of 96 h-LC50 (0.075 mg/L for T2 group) of phorate exposure for 15 and 30 d. The oxidative stress was assessed in terms of superoxide dismutase (SOD) and catalase (CAT) activities. DNA damage was measured as induction of micronuclei (MN) and consequent differential expression of apoptotic genes-tumor suppressor (p53), apoptotic peptidase activating factor-1 (apaf-1) and catalase (cat) in liver and kidney, two major sites of biotransformation in fish, were quantified...
May 3, 2017: Chemosphere
https://www.readbyqxmd.com/read/28505574/the-effect-of-insecticides-chlorpyrifos-%C3%AE-cypermethrin-and-imidacloprid-on-primary-dna-damage-tp-53-and-c-myc-structural-integrity-by-comet-fish-assay
#2
Davor Zeljezic, Benjamin Vinkovic, Vilena Kasuba, Nevenka Kopjar, Mirta Milic, Marin Mladinic
In parallel with the continuous use of conventional insecticides, introduction of more environmentally friendly substances continues to grow in modern agriculture. In the present study, we evaluated chlorpyrifos, and imidacloprid and α-cypermethrin as two representatives of green insecticides for their genotoxic activity. We conducted a 14-day treatment in extended human lymphocytes cultures using real life exposure relevant concentrations. An alkaline comet assay was used to detect primary DNA damage. Simultaneously, the effect on the specific action towards the TP 53 and c-Myc genes in terms of fragmentation and copy number were determined...
May 3, 2017: Chemosphere
https://www.readbyqxmd.com/read/28494285/effects-of-in-situ-exposure-to-tritiated-natural-environments-a-multi-biomarker-approach-using-the-fathead-minnow-pimephales-promelas
#3
B Gagnaire, C Adam-Guillermin, A Festarini, I Cavalié, C Della-Vedova, C Shultz, S B Kim, H Ikert, C Dubois, S Walsh, F Farrow, D Beaton, E Tan, K Wen, M Stuart
Aquatic ecosystems are chronically exposed to radionuclides as well as other pollutants. Increased concentrations of pollutants in aquatic environments can present a risk to exposed organisms, including fish. The goal of this study was to characterize the effects of tritium, in the context of natural environments, on the health of fathead minnow, Pimephales promelas. Fish were exposed to tritium (activity concentrations ranging from 2 to 23,000Bq/L) and also to various concentrations of several metals to replicate multiple-stressor environments...
May 8, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28490783/multiple-biomarker-responses-serum-biochemistry-oxidative-stress-genotoxicity-and-histopathology-in-channa-punctatus-exposed-to-heavy-metal-loaded-waste-water
#4
Mehjbeen Javed, Md Irshad Ahmad, Nazura Usmani, Masood Ahmad
Experiments were conducted to investigate the health of fish Channa punctatus inhabiting heavy metal-loaded waste water. Heavy metals in the order of Fe > Mn > Zn > Co > Ni > Cu = Cr were present in the waste water. Gills had high metal load followed by liver and then kidney. Albumin, albumin to globulin (A:G) ratio, triglyceride, high density lipoprotein (HDL) and very low density lipoprotein (VLDL) were found to be lower but phospholipid, low density lipoprotein (LDL), total protein, lipid and cholesterol were higher as compared to the reference...
May 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28486571/ras-oncogene-and-hypoxia-inducible-factor-1-alpha-hif-1%C3%AE-expression-in-the-amazon-fish-colossoma-macropomum-cuvier-1818-exposed-to-benzo-a-pyrene
#5
Grazyelle Sebrenski da Silva, Luciana Mara Lopes Fé, Maria de Nazaré Paula da Silva, Vera Maria Fonseca de Almeida E Val
Benzo[a]pyrene (B[a]P) is a petroleum derivative capable of inducing cancer in human and animals. In this work, under laboratory conditions, we analyzed the responses of Colossoma macropomum to B[a]P acute exposure through intraperitoneal injection of four different B[a]P concentrations (4, 8, 16 and 32 μmol/kg) or corn oil (control group). We analyzed expression of the ras oncogene and the Hypoxia-inducible factor-1 alpha (hif-1α) gene using quantitative real-time PCR. Additionally, liver histopathological changes and genotoxic effects were evaluated through the comet assay...
May 8, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28482225/immunostaining-of-uva-induced-dna-damage-in-erythrocytes-of-medaka-oryzias-latipes
#6
Alaa El-Din Hamid Sayed, Hiroshi Mitani
Some authors have recently reported that UVA induces double-strand breaks (DSBs) in DNA. Only a few researchers have reported on the induction of DSBs upon UVA exposure, as measured using the Comet assay and γ-H2AX as markers of DSB formation. In the present study, we have investigated for the first time the dose-dependent induction of DSBs by UVA in medaka (Oryzias latipes) erythrocytes. Adult female medaka fish were exposed to UVA for 15, 30, and 60min/day for three continuous days; an unirradiated control group was kept in the same laboratory conditions...
April 28, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28437728/is-a-a-o-process-effective-in-toxicity-removal-case-study-with-coking-wastewater
#7
Liu Shi, Dong Wang, Di Cao, Chunhong Na, Xie Quan, Ying Zhang
The anaerobic-anoxic-oxic (A/A/O) process is the commonly used biological wastewater treatment process, especially for the coking wastewater. However, limit is known about its ability in bio-toxicity removal from wastewater. In this study, we evaluated the performance of A/A/O process in bio-toxicity removal from the coking wastewater, using two test species (i.e. crustacean (Daphnia magna) and zebra fish (Danio rerio)) in respect of acute toxicity, oxidative damage and genotoxicity. Our results showed that the acute toxicity of raw influent was reduced gradually along with A/A/O process and the effluent presented no acute toxicity to Daphnia magna (D...
August 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28384500/bioaccumulation-of-trace-metals-and-total-petroleum-and-genotoxicity-responses-in-an-edible-fish-population-as-indicators-of-marine-pollution
#8
Avelyno D'Costa, S K Shyama, M K Praveen Kumar
The present study reports the genetic damage and the concentrations of trace metals and total petroleum hydrocarbons prevailing in natural populations of an edible fish, Arius arius in different seasons along the coast of Goa, India as an indicator of the pollution status of coastal water. Fish were collected from a suspected polluted site and a reference site in the pre-monsoon, monsoon and post-monsoon seasons. Physico-chemical parameters as well as the concentrations of total petroleum hydrocarbons (TPH) and trace metals in the water and sediment as well as the tissues of fish collected from these sites were recorded...
August 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28360001/assessment-of-urban-stream-sediment-pollutants-entering-estuaries-using-chemical-analysis-and-multiple-bioassays-to-characterise-biological-activities
#9
Svenja Boehler, Ruben Strecker, Patrick Heinrich, Erik Prochazka, Grant L Northcott, James M Ataria, Frederic D L Leusch, Thomas Braunbeck, Louis A Tremblay
Stormwater contaminants are a major source of often neglected environmental stressors because of the emphasis placed on the management of municipal and industrial wastewaters. Stormwater-derived pollutants in sediments from two New Zealand estuaries was characterised by analytical chemistry and bioassays. Contaminants were extracted from sediment using accelerated solvent extraction (ASE), recovered and concentrated by solid phase extraction (SPE), and analysed for polycyclic aromatic hydrocarbons (PAHs), selected metals, and musk fragrances...
March 27, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28347471/double-strand-break-repair-and-%C3%AE-h2ax-formation-in-erythrocytes-of-medaka-oryzias-latipes-after-%C3%AE-irradiation
#10
Alaa El-Din Hamid Sayed, Kento Igarashi, Tomomi Watanabe-Asaka, Hiroshi Mitani
The study of the DNA damage response in erythrocytes after γ-irradiation may provide evidence for its effectiveness as a biomarkers for genotoxic environmental stress. We previously reported various malformations in erythrocytes of medaka irradiated with10 Gy, but not in their micronuclei. In this study, we optimized an assay method for γ-H2AX and double strand breaks in erythrocytes of adult medaka fish after 15 Gy of γ-irradiation. The highest level of apoptosis and nuclear abnormalities, including in micronuclei, were recorded 4 h after γ-irradiation, as was the highest level of γ-H2AX foci in erythrocytes...
May 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28332630/pml-nuclear-bodies-contribute-to-the-basal-expression-of-the-mtor-inhibitor-ddit4
#11
Jayme Salsman, Alex Stathakis, Ellen Parker, Dudley Chung, Livia E Anthes, Kara L Koskowich, Sara Lahsaee, Daniel Gaston, Kimberly R Kukurba, Kevin S Smith, Ian C Chute, Daniel Léger, Laura D Frost, Stephen B Montgomery, Stephen M Lewis, Christopher Eskiw, Graham Dellaire
The promyelocytic leukemia (PML) protein is an essential component of PML nuclear bodies (PML NBs) frequently lost in cancer. PML NBs coordinate chromosomal regions via modification of nuclear proteins that in turn may regulate genes in the vicinity of these bodies. However, few PML NB-associated genes have been identified. PML and PML NBs can also regulate mTOR and cell fate decisions in response to cellular stresses. We now demonstrate that PML depletion in U2OS cells or TERT-immortalized normal human diploid fibroblasts results in decreased expression of the mTOR inhibitor DDIT4 (REDD1)...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28324828/evaluation-of-dna-damage-and-physiological-responses-in-nile-tilapia-oreochromis-niloticus-linnaeus-1758-exposed-to-sub-lethal-diclofenac-dcf
#12
Pramod K Pandey, Malachy N O Ajima, Kundan Kumar, Nalini Poojary, Saurav Kumar
The frequent bioaccumulation of pharmaceuticals in the aquatic ecosystem has raised a concern about their possible ecotoxicological consequences. DNA damage, haematological changes and activities of oxidative stress enzymes in Nile tilapia, Oreochromis niloticus in response to diclofenac (DCF) exposure were investigated for up to 60 days at the concentrations of 0.17, 0.34 and 0.68mgL(-1) in the fish liver. Evaluation of genotoxic effects of the drug in the liver, using single-cell gel electrophoresis, showed DNA damage on exposure at the concentrations of 0...
March 8, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28316899/water-quality-assessment-in-the-german-river-of-the-years-2014-2015-how-a-case-study-on-the-impact-of-a-storm-water-sedimentation-basin-displayed-impairment-of-fish-health-in-the-argen-river-southern-germany
#13
Paul Thellmann, Bertram Kuch, Karl Wurm, Heinz-R Köhler, Rita Triebskorn
BACKGROUND: The present work investigates the impact of discharges from a storm water sedimentation basin (SSB) receiving runoff from a connected motorway in southern Germany. The study lasted for almost two years and was aimed at assessing the impact of the SSB on the fauna of the Argen River, which is a tributary of Lake Constance. Two sampling sites were examined up- and downstream of the SSB effluent. A combination of different diagnostic methods (fish embryo test with the zebrafish, histopathology, micronucleus test) was applied to investigate health impairment and genotoxic effects in indigenous fish as well as embryotoxic potentials in surface water and sediment samples of the Argen River, respectively, in samples of the SSB effluent...
2017: Environmental Sciences Europe
https://www.readbyqxmd.com/read/28303538/widely-used-non-ionic-surfactant-4-nonylphenol-showing-genotoxic-effects-in-various-tissues-of-channa-punctatus
#14
Madhu Sharma, Pooja Chadha
The present study investigated the intertissue differences in genotoxicity induction in the fish, Channa punctatus, in response to 4-nonylphenol. The lethal concentration of 50 (LC50) was estimated using a semi-static system. Fish were subjected to three sublethal concentrations of 4-nonylphenol (NP) for 24, 48, 72, and 96 h. Liver, gill, and kidney tissues were analyzed, and the genotoxicity was estimated using the micronucleus test and the comet assay. The frequency of micronucleated cells (MNCs), binucleated cells (BNCs), aberrant cells, and tail moment (TM) increased significantly...
March 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28281072/frequencies-of-erythrocyte-nuclear-abnormalities-and-of-leucocytes-in-the-fish-barbus-peloponnesius-correlate-with-a-pollution-gradient-in-the-river-bregalnica-macedonia
#15
Katerina Rebok, Maja Jordanova, Valentina Slavevska-Stamenković, Lozenka Ivanova, Vasil Kostov, Trajče Stafilov, Eduardo Rocha
Integrated chemical and biomarker approaches were performed to estimate if there is ongoing toxicity in the River Bregalnica, namely connected with the presence of metals. The study was performed in water, sediment, and barbel (Barbus peloponnesius), collected in two seasons, from two suspected polluted and one reference zones. The water analyses revealed higher mean values in polluted sites for most of the examined physicochemical parameters. Metal concentrations (Zn, Cu, Cd, Mn, Pb, and Fe) in water were more or less constant, whereas in sediment, they were higher at the two polluted locations...
March 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28263526/bioindicators-of-wastewater-ecotoxicity
#16
REVIEW
Gabriela Jirova, Zdenka Wittlingerova, Magdalena Zimova, Alena Vlkova, Martina Wittlerova, Marketa Dvorakova, Dagmar Jirova
Wastewater, especially containing hospital effluents, exhibits high chemical complexity and specificity since it includes various chemicals, biocides, pharmaceuticals, surfactants, radionuclides, disinfectants and pathogens. Biological tests provide true evidence of the wastewater quality and unlike chemical analytical tests show comprehensive pollution effects on the environment and human health. Normalized conventional bioassays are not sensitive enough for ecotoxicological evaluation of wastewater and there is a great need for the development of suitable sensitive bioassays in order to characterize properly the residual toxicity of treated effluents...
December 18, 2016: Neuro Endocrinology Letters
https://www.readbyqxmd.com/read/28242497/toxicological-effects-of-graphene-oxide-on-adult-zebrafish-danio-rerio
#17
Jaqueline P Souza, Jéssica F Baretta, Fabrício Santos, Ieda M M Paino, Valtencir Zucolotto
Graphene exhibits unique physical and chemical properties that facilitate its application in many fields, including electronics and biomedical areas. However, the use of graphene and its derivatives could result in accumulation in aquatic environments, and the risks posed by these compounds for organisms are not completely understood. In this study, we investigated the effects of graphene oxide (GO) on adult zebrafish (Danio rerio). Experimental fish were exposed to 2, 10 or 20mgL(-1) GO, and the cytotoxicity, genotoxicity and oxidative stress were assessed...
February 16, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28213766/microelectrophoretic-study-of-environmentally-induced-dna-damage-in-fish-and-its-use-for-early-toxicity-screening-of-freshwater-bodies
#18
Bilal Hussain, Tayyaba Sultana, Salma Sultana, K A Al-Ghanim, Shahreef Masood, Muhammad Ali, Shahid Mahboob
This study investigates the potential of the comet and micronucleus assays of fish DNA as a means of screening the toxicity of aquatic environments. Catla catla and Cirrhinus mrigala collected from the River Chenab in Pakistan were used as a case study for the application of comet and micronucleus techniques. Comet and micronucleus assays were used to compare DNA damage in C. catla and C. mrigala collected from polluted areas of the River Chenab and farmed fish. Atomic absorption spectrophotometry showed an acute level of toxicity from Cd, Cu, Mn, Zn, Pb, Cr, Sn, and Hg in river water...
March 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28185655/oxidative-stress-and-genotoxicity-biomarker-responses-in-tilapia-oreochromis-niloticus-exposed-to-environmental-concentration-of-1-nitropyrene
#19
Eugene T Bacolod, Seiichi Uno, Shiela S Villamor, Jiro Koyama
The present study aimed to assess whether environmental 1-nitropyrene (1-NP) concentration will induce genotoxicity and oxidative damages in tilapia, lives in estuarine and brackish water. Tilapia were exposed to waterborne 1-NP. Cellular antioxidant enzyme activity of glutathione peroxidase and oxidative damage, i.e., lipid peroxidation, protein and DNA oxidation were used as biomarkers of oxidative stress, while the micronucleus test was used for evaluation of chromosomal damage and was used as an indication of genotoxicity...
February 6, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28167441/effects-of-environmentally-relevant-concentrations-of-the-anti-inflammatory-drug-diclofenac-in-freshwater-fish-rhamdia-quelen
#20
Izonete Cristina Guiloski, Laercio Dante Stein Piancini, Ana Carolina Dagostim, Sabrina Loise de Morais Calado, Luis Fernando Fávaro, Suelen Lúcio Boschen, Marta Margarete Cestari, Cláudio da Cunha, Helena Cristina Silva de Assis
The presence of pharmaceuticals in the aquatic environment and its impact on humans and the ecosystem are emerging issues in environmental health. This study evaluated the potential biochemical, genetic and reproductive effects of the diclofenac by waterborne exposure, in a semi-static bioassay for 21 days. The fish Rhamdia quelen were exposed to environmental concentrations of diclofenac (0, 0.2, 2 and 20µg/L). The results showed that in the liver, diclofenac reduced the catalase and ethoxyresorufin- O- deethylase activities in fish exposed to 2µg/L, and superoxide dismutase in all exposed groups...
February 3, 2017: Ecotoxicology and Environmental Safety
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