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Genotoxicity of Fishes

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https://www.readbyqxmd.com/read/28332630/pml-nuclear-bodies-contribute-to-the-basal-expression-of-the-mtor-inhibitor-ddit4
#1
Jayme Salsman, Alex Stathakis, Ellen Parker, Dudley Chung, Livia E Anthes, Kara L Koskowich, Sara Lahsaee, Daniel Gaston, Kimberly R Kukurba, Kevin S Smith, Ian C Chute, Daniel Léger, Laura D Frost, Stephen B Montgomery, Stephen M Lewis, Christopher Eskiw, Graham Dellaire
The promyelocytic leukemia (PML) protein is an essential component of PML nuclear bodies (PML NBs) frequently lost in cancer. PML NBs coordinate chromosomal regions via modification of nuclear proteins that in turn may regulate genes in the vicinity of these bodies. However, few PML NB-associated genes have been identified. PML and PML NBs can also regulate mTOR and cell fate decisions in response to cellular stresses. We now demonstrate that PML depletion in U2OS cells or TERT-immortalized normal human diploid fibroblasts results in decreased expression of the mTOR inhibitor DDIT4 (REDD1)...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28324828/evaluation-of-dna-damage-and-physiological-responses-in-nile-tilapia-oreochromis-niloticus-linnaeus-1758-exposed-to-sub-lethal-diclofenac-dcf
#2
Pramod K Pandey, Malachy N O Ajima, Kundan Kumar, Nalini Poojary, Saurav Kumar
The frequent bioaccumulation of pharmaceuticals in the aquatic ecosystem has raised a concern about their possible ecotoxicological consequences. DNA damage, haematological changes and activities of oxidative stress enzymes in Nile tilapia, Oreochromis niloticus in response to diclofenac (DCF) exposure were investigated for up to 60 days at the concentrations of 0.17, 0.34 and 0.68mgL(-1) in the fish liver. Evaluation of genotoxic effects of the drug in the liver, using single-cell gel electrophoresis, showed DNA damage on exposure at the concentrations of 0...
March 8, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28316899/water-quality-assessment-in-the-german-river-of-the-years-2014-2015-how-a-case-study-on-the-impact-of-a-storm-water-sedimentation-basin-displayed-impairment-of-fish-health-in-the-argen-river-southern-germany
#3
Paul Thellmann, Bertram Kuch, Karl Wurm, Heinz-R Köhler, Rita Triebskorn
BACKGROUND: The present work investigates the impact of discharges from a storm water sedimentation basin (SSB) receiving runoff from a connected motorway in southern Germany. The study lasted for almost two years and was aimed at assessing the impact of the SSB on the fauna of the Argen River, which is a tributary of Lake Constance. Two sampling sites were examined up- and downstream of the SSB effluent. A combination of different diagnostic methods (fish embryo test with the zebrafish, histopathology, micronucleus test) was applied to investigate health impairment and genotoxic effects in indigenous fish as well as embryotoxic potentials in surface water and sediment samples of the Argen River, respectively, in samples of the SSB effluent...
2017: Environmental Sciences Europe
https://www.readbyqxmd.com/read/28303538/widely-used-non-ionic-surfactant-4-nonylphenol-showing-genotoxic-effects-in-various-tissues-of-channa-punctatus
#4
Madhu Sharma, Pooja Chadha
The present study investigated the intertissue differences in genotoxicity induction in the fish, Channa punctatus, in response to 4-nonylphenol. The lethal concentration of 50 (LC50) was estimated using a semi-static system. Fish were subjected to three sublethal concentrations of 4-nonylphenol (NP) for 24, 48, 72, and 96 h. Liver, gill, and kidney tissues were analyzed, and the genotoxicity was estimated using the micronucleus test and the comet assay. The frequency of micronucleated cells (MNCs), binucleated cells (BNCs), aberrant cells, and tail moment (TM) increased significantly...
March 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28281072/frequencies-of-erythrocyte-nuclear-abnormalities-and-of-leucocytes-in-the-fish-barbus-peloponnesius-correlate-with-a-pollution-gradient-in-the-river-bregalnica-macedonia
#5
Katerina Rebok, Maja Jordanova, Valentina Slavevska-Stamenković, Lozenka Ivanova, Vasil Kostov, Trajče Stafilov, Eduardo Rocha
Integrated chemical and biomarker approaches were performed to estimate if there is ongoing toxicity in the River Bregalnica, namely connected with the presence of metals. The study was performed in water, sediment, and barbel (Barbus peloponnesius), collected in two seasons, from two suspected polluted and one reference zones. The water analyses revealed higher mean values in polluted sites for most of the examined physicochemical parameters. Metal concentrations (Zn, Cu, Cd, Mn, Pb, and Fe) in water were more or less constant, whereas in sediment, they were higher at the two polluted locations...
March 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28263526/bioindicators-of-wastewater-ecotoxicity
#6
Gabriela Jirova, Zdenka Wittlingerova, Magdalena Zimova, Alena Vlkova, Martina Wittlerova, Marketa Dvorakova, Dagmar Jirova
Wastewater, especially containing hospital effluents, exhibits high chemical complexity and specificity since it includes various chemicals, biocides, pharmaceuticals, surfactants, radionuclides, disinfectants and pathogens. Biological tests provide true evidence of the wastewater quality and unlike chemical analytical tests show comprehensive pollution effects on the environment and human health. Normalized conventional bioassays are not sensitive enough for ecotoxicological evaluation of wastewater and there is a great need for the development of suitable sensitive bioassays in order to characterize properly the residual toxicity of treated effluents...
December 18, 2016: Neuro Endocrinology Letters
https://www.readbyqxmd.com/read/28242497/toxicological-effects-of-graphene-oxide-on-adult-zebrafish-danio-rerio
#7
Jaqueline P Souza, Jéssica F Baretta, Fabrício Santos, Ieda M M Paino, Valtencir Zucolotto
Graphene exhibits unique physical and chemical properties that facilitate its application in many fields, including electronics and biomedical areas. However, the use of graphene and its derivatives could result in accumulation in aquatic environments, and the risks posed by these compounds for organisms are not completely understood. In this study, we investigated the effects of graphene oxide (GO) on adult zebrafish (Danio rerio). Experimental fish were exposed to 2, 10 or 20mgL(-1) GO, and the cytotoxicity, genotoxicity and oxidative stress were assessed...
February 16, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28213766/microelectrophoretic-study-of-environmentally-induced-dna-damage-in-fish-and-its-use-for-early-toxicity-screening-of-freshwater-bodies
#8
Bilal Hussain, Tayyaba Sultana, Salma Sultana, K A Al-Ghanim, Shahreef Masood, Muhammad Ali, Shahid Mahboob
This study investigates the potential of the comet and micronucleus assays of fish DNA as a means of screening the toxicity of aquatic environments. Catla catla and Cirrhinus mrigala collected from the River Chenab in Pakistan were used as a case study for the application of comet and micronucleus techniques. Comet and micronucleus assays were used to compare DNA damage in C. catla and C. mrigala collected from polluted areas of the River Chenab and farmed fish. Atomic absorption spectrophotometry showed an acute level of toxicity from Cd, Cu, Mn, Zn, Pb, Cr, Sn, and Hg in river water...
March 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28185655/oxidative-stress-and-genotoxicity-biomarker-responses-in-tilapia-oreochromis-niloticus-exposed-to-environmental-concentration-of-1-nitropyrene
#9
Eugene T Bacolod, Seiichi Uno, Shiela S Villamor, Jiro Koyama
The present study aimed to assess whether environmental 1-nitropyrene (1-NP) concentration will induce genotoxicity and oxidative damages in tilapia, lives in estuarine and brackish water. Tilapia were exposed to waterborne 1-NP. Cellular antioxidant enzyme activity of glutathione peroxidase and oxidative damage, i.e., lipid peroxidation, protein and DNA oxidation were used as biomarkers of oxidative stress, while the micronucleus test was used for evaluation of chromosomal damage and was used as an indication of genotoxicity...
February 6, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28167441/effects-of-environmentally-relevant-concentrations-of-the-anti-inflammatory-drug-diclofenac-in-freshwater-fish-rhamdia-quelen
#10
Izonete Cristina Guiloski, Laercio Dante Stein Piancini, Ana Carolina Dagostim, Sabrina Loise de Morais Calado, Luis Fernando Fávaro, Suelen Lúcio Boschen, Marta Margarete Cestari, Cláudio da Cunha, Helena Cristina Silva de Assis
The presence of pharmaceuticals in the aquatic environment and its impact on humans and the ecosystem are emerging issues in environmental health. This study evaluated the potential biochemical, genetic and reproductive effects of the diclofenac by waterborne exposure, in a semi-static bioassay for 21 days. The fish Rhamdia quelen were exposed to environmental concentrations of diclofenac (0, 0.2, 2 and 20µg/L). The results showed that in the liver, diclofenac reduced the catalase and ethoxyresorufin- O- deethylase activities in fish exposed to 2µg/L, and superoxide dismutase in all exposed groups...
February 3, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28167386/dna-adducts-in-marine-fish-as-biological-marker-of-genotoxicity-in-environmental-monitoring-the-way-forward
#11
REVIEW
Daniela M Pampanin, Steven J Brooks, Bjørn Einar Grøsvik, Jérémie Le Goff, Sonnich Meier, Magne O Sydnes
DNA adducts in fish represent a very important genotoxicity endpoint in environmental monitoring, being a pre-mutagenic lesion that plays an essential role in the initiation of carcinogenesis. The analysis of DNA adducts is a challenging task due to the low concentration of the analyte. Methods are available to determine the presence of DNA adducts, although further knowledge is required to fully understand the nature of the adducts and responsible xenobiotics (i.e. position of adduct in DNA, most active xenobiotic and metabolite forms, structural information)...
April 2017: Marine Environmental Research
https://www.readbyqxmd.com/read/28160022/role-of-human-sulfotransferase-1a1-and-n-acetyltransferase-2-in-the-metabolic-activation-of-16-heterocyclic-amines-and-related-heterocyclics-to-genotoxicants-in-recombinant-v79-cells
#12
Matthieu Chevereau, Hansruedi Glatt, Daniel Zalko, Jean-Pierre Cravedi, Marc Audebert
Heterocyclic aromatic amines (HAAs) are primarily produced during the heating of meat or fish. HAAs are mutagenic and carcinogenic, and their toxicity in model systems depend on metabolic activation. This activation is mediated by cytochrome P450 (CYP) enzymes, in particular CYP1A2. Some studies have indicated a role of human sulfotransferase (SULT) 1A1 and N-acetyltransferase (NAT) 2 in the terminal activation of HAAs. In this study, we conducted a metabolism/genotoxicity relationship analysis for 16 HAAs and related heterocyclics...
February 3, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28159303/transcriptional-responses-of-zebrafish-to-complex-metal-mixtures-in-laboratory-studies-overestimates-the-responses-observed-with-environmental-water
#13
Ajay Pradhan, Per Ivarsson, Daniel Ragnvaldsson, Håkan Berg, Jana Jass, Per-Erik Olsson
Metals released into the environment continue to be of concern for human health. However, risk assessment of metal exposure is often based on total metal levels and usually does not take bioavailability data, metal speciation or matrix effects into consideration. The continued development of biological endpoint analyses are therefore of high importance for improved eco-toxicological risk analyses. While there is an on-going debate concerning synergistic or additive effects of low-level mixed exposures there is little environmental data confirming the observations obtained from laboratory experiments...
April 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28140694/genotoxic-and-hematological-effects-of-chlorpyrifos-exposure-on-freshwater-fish-labeo-rohita
#14
Muhammad Ismail, Rahat Ali, Muhammad Shahid, Muhammad Asaf Khan, Muhammad Zubair, Tayyaba Ali, Qaiser Mahmood Khan
Chlorpyrifos is a commonly used organophosphate insecticide that causes toxicological effects in aquatic organisms especially in fish. This study determined the effects of chlorpyrifos on the genotoxic and hematological parameters of freshwater fish, Labeo rohita. The genotoxic effects of different sublethal concentrations of chlorpyrifos were investigated in the erythrocytes of Labeo rohita (commonly known as Rohu) using the Micronucleus test. Effects of chlorpyrifos on the hematological parameters of the fish were also observed...
January 31, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28137363/assessment-of-the-genotoxicity-of-the-tyrosine-kinase-inhibitor-imatinib-mesylate-in-cultured-fish-and-human-cells
#15
Matjaž Novak, Bojana Žegura, Jana Nunić, Goran Gajski, Marko Gerić, Vera Garaj-Vrhovac, Metka Filipič
The selective tyrosine kinase inhibitor imatinib mesylate (IM) is a widely used anticancer drug. Recent studies showing that IM can induce DNA and chromosomal damage in crustaceans and higher plants prompted us to re-examine its potential genotoxicity. IM was not mutagenic in the Ames assay (Salmonella typhimurium). Cytotoxicity and genotoxicity were evaluated in vitro in zebrafish (Danio rerio) liver (ZFL), human hepatoma (HepG2), and human peripheral blood lymphocyte (HPBL) cells. Genotoxicity was determined with the comet assay and with the cytokinesis-block micronucleus cytome assay...
February 2017: Mutation Research
https://www.readbyqxmd.com/read/28131919/comet-assay-and-micronucleus-tests-on-oreochromis-niloticus-perciforme-cichlidae-exposed-to-raw-sugarcane-vinasse-and-to-phisicochemical-treated-vinasse-by-ph-adjustment-with-lime-cao
#16
Jorge E Correia, Cintya Ap Christofoletti, Yadira Ansoar-Rodríguez, Thays A Guedes, Carmem S Fontanetti
In Brazil vinasse, a main sugarcane distillery residue, stands out because every liter of alcohol generates 10-15 L of vinasse as waste. An alternative for the disposal of this waste is the fertirrigation of the sugarcane culture itself. However, the high amount released can saturate the soil and through leaching/percolation contaminate water resources. The aim of this study is verifying the toxic potential of vinasse in tilapias and effectiveness of the physicalchemical treatment of this waste with pH adjustment with lime (CaO)...
January 26, 2017: Chemosphere
https://www.readbyqxmd.com/read/28118403/brachypodium-distachyon-a-useful-model-in-the-qualification-of-mutagen-induced-micronuclei-using-multicolor-fish
#17
Arita Kus, Jolanta Kwasniewska, Robert Hasterok
Brachypodium distachyon (Brachypodium) is now intensively utilized as a model grass species in various biological studies. Its favorable cytological features create a unique foundation for a convenient system in mutagenesis, thereby potentially enabling the 'hot spots' and 'cold spots' of DNA damage in its genome to be analyzed. The aim of this study was to analyze the involvement of 5S rDNA, 25S rDNA, the Arabidopsis-type (TTTAGGG)n telomeric sequence and the Brachypodium-originated centromeric BAC clone CB33J12 in the micronuclei formation in Brachypodium root tip cells that were subjected to the chemical clastogenic agent maleic hydrazide (MH)...
2017: PloS One
https://www.readbyqxmd.com/read/28091988/genotoxicity-and-cytotoxicity-reduction-of-the-polluted-urban-river-after-ecological-restoration-a-field-scale-study-of-jialu-river-in-northern-china
#18
Jie Sun, Rui Zhang, Long Qin, Haixiao Zhu, Yu Huang, Yingang Xue, Shuqing An, Xianchuan Xie, Aimin Li
To further treat the reclaimed municipal wastewater and rehabilitate the aquatic ecosystem of polluted urban rivers, an 18.5-km field-scale ecological restoration project was constructed along Jialu River, a polluted urban river which receives only reclaimed municipal wastewater from Zhengzhou City without natural upland water dilution. This study investigated the potential efficiency of water quality improvement, as well as genotoxicity and cytotoxicity reduction along the ecological restoration project of this polluted urban river...
January 13, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28055311/immunotoxicity-of-mercury-pathological-and-toxicological-effects
#19
Faheem Maqbool, Kamal Niaz, Fatima Ismail Hassan, Fazlullah Khan, Mohammad Abdollahi
Mercury (Hg) is toxic and hazardous metal that causes natural disasters in the earth's crust. Exposure to Hg occurs via various routes; like oral (fish), inhalation, dental amalgams, and skin from cosmetics. In this review, we have discussed the sources of Hg and its potential for causing toxicity in humans. In addition, we also review its bio-chemical cycling in the environment; its systemic, immunotoxic, genotoxic/carcinogenic, and teratogenic health effects; and the dietary influences; as well as the important considerations in risk assessment and management of Hg poisoning have been discussed in detail...
January 2, 2017: Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews
https://www.readbyqxmd.com/read/28040614/recommendation-for-a-test-battery-for-the-ecotoxicological-evaluation-of-the-environmental-safety-of-construction-products
#20
Stefan Gartiser, Ines Heisterkamp, Ute Schoknecht, Nicole Bandow, N Michael Burkhardt, Monika Ratte, Outi Ilvonen
The European Construction Products Regulation allows Member States to adopt rules for evaluating the environmental impact of their buildings. The aim of the project was to develop recommendations for a test battery for the ecotoxicological assessment of the environmental impact of construction products for outdoor use and contribute to the European harmonization of test methods. From a shortlist of 39 products 20 products were included in the ecotoxicological testing program. Monolithic and plate-like construction products were eluted in the Dynamic Surface Leaching test (DSLT) in accordance with CEN/TS 16637-2, granular products were eluted in a one stage batch test in accordance with DIN EN 12457-1...
December 23, 2016: Chemosphere
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