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Genotoxicity of Fishes

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https://www.readbyqxmd.com/read/28641238/water-soluble-fraction-of-petroleum-induces-genotoxicity-and-morphological-effects-in-fat-snook-centropomus-parallelus
#1
Laila Carine Campos Medeiros, Frederico Augusto Cariello Delunardo, Larissa Novaes Simões, Marcelo Gustavo Paulino, Thais Souza Vargas, Marisa Narciso Fernandes, Rodrigo Scherer, Adriana Regina Chippari-Gomes
Petroleum hydrocarbons are one of the primary organic chemicals found in water bodies, and the water-soluble fraction of petroleum (WSFP) may be responsible for much of the toxic effects. In the present study, genotoxicity assays and histopathological analysis of the gills were analyzed for two experimental protocols: 1) Juvenile Centropomus parallelus were exposed to different concentrations of WFSP (0%, 25%, 50% and 75%) for 96h; 2) A second fish group was exposed to 50% WFSP for 168h followed by a post-exposure period for 168h in clean water (recovery)...
June 19, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28622554/evaluation-of-dna-damage-induced-by-gamma-radiation-in-gill-and-muscle-tissues-of-cyprinus-carpio-and-their-relative-sensitivity
#2
Praveen Kumar M K, Soorambail K Shyama, Avelyno D'Costa, Samit B Kadam, Bhagatsingh Harisingh Sonaye, Ramesh Chandra Chaubey
The effect of radiation on the aquatic environment is of major concern in recent years. Limited data is available on the genotoxicity of gamma radiation on different tissues of aquatic organisms. Hence, the present investigation was carried out to study the DNA damage induced by gamma radiation in the gill and muscle tissues and their relative sensitivity using the comet assay in the freshwater teleost fish, common carp (Cyprinus carpio). The comet assay was optimized and validated in common carp using cyclophosphamide (CP), a reference genotoxic agent...
June 13, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28601519/cytotoxic-and-genotoxic-affects-of-acid-mine-drainage-on-fish-channa-punctata-bloch
#3
B Talukdar, H K Kalita, S Basumatary, D J Saikia, D Sarma
The investigation deals with the effects of Acid Mine Drainage (AMD) of coal mine on fish Channa punctata (Bloch) by examining the incidence of haematological, morphological, histological changes and DNA fragmentation in tissues of C. punctata in laboratory condition. For this study fishes were exposed to 10% of AMD for a period of 30 days. The fusion of the primary and secondary gill lamellae, distortion, loss of alignment, deposition of worn out tissues and mucous on the surface of the lamella in the gills; degeneration of morphological architecture, loss of alignment of tubules, mucous deposition in the kidney; cellular damage, cellular necrosis, extraneous deposition on the surface, pore formation in the liver are some important changes detected by scanning electron microscopy...
June 8, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28583304/transgenerational-inheritance-of-neurobehavioral-and-physiological-deficits-from-developmental-exposure-to-benzo-a-pyrene-in-zebrafish
#4
Andrea L Knecht, Lisa Truong, Skylar W Marvel, David M Reif, Abraham Garcia, Catherine Lu, Michael T Simonich, Justin G Teeguarden, Robert L Tanguay
Benzo[a]pyrene (B[a]P) is a well-known genotoxic polycylic aromatic compound whose toxicity is dependent on signaling via the aryl hydrocarbon receptor (AHR). It is unclear to what extent detrimental effects of B[a]P exposures might impact future generations and whether transgenerational effects might be AHR-dependent. This study examined the effects of developmental B[a]P exposure on 3 generations of zebrafish. Zebrafish embryos were exposed from 6 to 120h post fertilization (hpf) to 5 and 10μM B[a]P and raised in chemical-free water until adulthood (F0)...
June 3, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28575799/application-of-fish-cell-lines-for-evaluating-the-chromium-induced-cytotoxicity-genotoxicity-and-oxidative-stress
#5
G Taju, S Abdul Majeed, K S N Nambi, A S Sahul Hameed
In the present study, we hypothesize that cytotoxicity, genotoxicity and oxidative stress play a key role in chromium induced toxicity in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines after 24 h exposure. Three fish species namely Lates calcarifer, Etroplus suratensis and Catla catla were exposed to the concentrations of 0, 10, 20, 30, 40 and 50 mg/L of chromium for 96 h under static conditions for conducting acute toxicity tests. LC50 was then calculated. The percentage cell survival was assessed by multiple endpoints such as MTT, NR, AB and CB assays in the seven fish cell lines exposed to different concentrations of chromium and EC50 values of all the four endpoints were calculated...
May 27, 2017: Chemosphere
https://www.readbyqxmd.com/read/28573396/toxicity-assessment-of-magnetosomes-in-different-models
#6
T Revathy, M A Jayasri, K Suthindhiran
Magnetosomes are nanosized iron oxide particles surrounded by lipid membrane synthesized by magnetotactic bacteria (MTB). Magnetosomes have been exploited for a broad range of biomedical and biotechnological applications. Due to their enormous potential in the biomedical field, its safety assessment is necessary. Detailed research on the toxicity of the magnetosomes was not studied so far. This study focuses on the toxicity assessment of magnetosomes in various models such as Human RBC's, WBC's, mouse macrophage cell line (J774), Onion root tip and fish (Oreochromis mossambicus)...
June 2017: 3 Biotech
https://www.readbyqxmd.com/read/28554110/evaluation-of-the-detoxification-efficiencies-of-coking-wastewater-treated-by-combined-anaerobic-anoxic-oxic-a-2-o-and-advanced-oxidation-process
#7
Chunhong Na, Ying Zhang, Xie Quan, Shuo Chen, Wei Liu, Yaobin Zhang
Coking wastewater contains many types of toxic and hazardous pollutants that have serious toxic effects on human beings as well as aquatic organisms. However, few studies have evaluated the detoxification efficiencies of the treatment processes that are extensively performed in operational coking wastewater treatment plants (WWTPs). This study investigates the detoxification efficiencies of a combined anaerobic-anoxic-oxic (A(2)O)-ozonation and A(2)O-Fenton oxidation process in two coking WWTPs using an acute immobilization test for Daphnia magna, acute toxicity test for adult zebrafish, embryo toxicity test for zebrafish and the comet assay...
May 22, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28551207/genotoxic-and-mutagenic-assessment-of-iron-oxide-maghemite-%C3%AE-fe2o3-nanoparticle-in-the-guppy-poecilia-reticulata
#8
Gabriel Qualhato, Thiago Lopes Rocha, Emília Celma de Oliveira Lima, Daniela Melo E Silva, Júlio Roquete Cardoso, Cesar Koppe Grisolia, Simone Maria Teixeira de Sabóia-Morais
The environmental risk of nanomaterials (NMs) designed and used in nanoremediation process is of emerging concern, but their ecotoxic effects to aquatic organism remains unclear. In this study, the citrate-coated (maghemite) nanoparticles (IONPs) were synthesized and its genotoxic and mutagenic effects were investigated in the female guppy Poecilia reticulata. Fish were exposed to IONPs at environmentally relevant iron concentration (0.3 mg L(-1)) during 21 days and the animals were collected at the beginning of the experiment and after 3, 7, 14 and 21 days of exposure...
May 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28545014/paracetamol-causes-endocrine-disruption-and-hepatotoxicity-in-male-fish-rhamdia-quelen-after-subchronic-exposure
#9
Izonete Cristina Guiloski, João Luiz Coelho Ribas, Laercio Dante Stein Piancini, Ana Carolina Dagostim, Silvana Maris Cirio, Luis Fernando Fávaro, Suelen Lúcio Boschen, Marta Margarete Cestari, Cláudio da Cunha, Helena Cristina Silva de Assis
Paracetamol is one of the most widely sold non-prescription drugs. This study aimed to evaluate the effects of the paracetamol on reproductive, biochemical, genetic, histopathological and hematogical biomarkers by waterborne exposure. Male fish of Rhamdia quelen were exposed to environmental concentrations of paracetamol (0, 0.25, 2.5μg/L) in a semi-static bioassay for 21days. Hemoglobin and hematocrit were reduced upon exposure to 0.25μg/L of paracetamol. Leukocytes and thrombocytes increased after paracetamol exposure...
May 8, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28540546/environmental-risk-assessment-in-five-rivers-of-parana-river-basin-southern-brazil-through-biomarkers-in-astyanax-spp
#10
Ivaldete Tijolin Barros, Juliana Parolin Ceccon, Andressa Glinski, Samuel Liebel, Sonia Regina Grötzner, Marco Antonio Ferreira Randi, Evanilde Benedito, Claudia Feijó Ortolani-Machado, Francisco Filipak Neto, Ciro Alberto de Oliveira Ribeiro
In the current study, water quality of five river sites in Parana River basin (Brazil), utilized for public water supply, was assessed through a set of biomarkers in fish Astyanax spp. Population growth and inadequate use of land are challenges to the preservation of biodiversity and resources such as water. Some physicochemical parameters as well as somatic indexes, gills and liver histopathology, genotoxicity, and biochemical biomarkers were evaluated. The highest gonadosomatic index (GSI) and antioxidant parameters (catalase and glutathione S-transferase activities, non-protein thiols), as well as the lowest damage to biomolecules (lipid peroxidation, protein carbonylation, DNA damage) were observed in site 0 (Piava River), which is located at an environmental protected area...
May 24, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28536798/pollutants-induced-oxidative-stress-dna-damage-and-cellular-deformities-in-clarias-gariepinus-burchell-from-river-yamuna-in-delhi-region-india
#11
Anil K Tyor, Kanika Pahwa
The purpose of the present study was to evaluate the severity of pollution impact at two extreme sites of river Yamuna in Delhi region utilizing oxidative stress, genotoxic and histopathological biomarkers in gonad (ovary) of Clarias gariepinus. To evaluate oxidative stress, TBAR's and ferric reducing antioxidant power (FRAP) assay were employed while comet assay and classic histology were used to estimate genotoxicity and cellular damage respectively. The results indicated significant increase (p < 0...
May 23, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28534072/genotoxic-assessment-of-some-inorganic-compounds-in-desert-pupfish-cyprinodon-macularius-in-the-evaporation-pond-from-a-geothermal-plant
#12
Miguel Flores-Galván, Evarista Arellano-García, Gorgonio Ruiz-Campos, Luis Walter Daesslé
The frequency of micro nucleated erythrocytes in peripheral blood of the desert pupfish (Cyprinodon macularius) from a geothermal effluent pond is determined and compared to organisms kept in an aquarium. The frequency of micronucleated erythrocytes found in pupfish from the geothermal pond is 2.75 (±2.09) and only 0.44 (±0.52) in captivity organisms. Dissolved As in the ponds doubles the 340 µg L(-1) US-EPA acute quality criteria for aquatic life and Hg equals the 1.77 µg L(-1) chronic criteria. The organisms with high MNE also have significantly high Se, As and Hg concentrations in muscle and liver...
May 22, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28531838/antimicrobial-agents-triclosan-chloroxylenol-methylisothiazolinone-and-borax-used-in-cleaning-had-genotoxic-and-histopathologic-effects-on-rainbow-trout
#13
Erol Capkin, Tuna Ozcelep, Sevki Kayis, Ilhan Altinok
Triclosan (TRC), chloroxylenol (PCMX) and methylisothiazolinone (MIT) have been commonly used as an antimicrobial in soaps while borax (BRX) is used in household cleaning. After using these chemicals, they are washed down drains and getting into the aquatic ecosystem in which they may affect aquatic living organisms. In the present study, the chronic effects of TRC, PCMX, MIT and BRX on genotoxicity, gene expression and histopathology of rainbow trout (Oncorhynchus mykiss) were evaluated for 40 days under semi static condition...
May 15, 2017: Chemosphere
https://www.readbyqxmd.com/read/28511133/phorate-induced-oxidative-stress-dna-damage-and-differential-expression-of-p53-apaf-1-and-cat-genes-in-fish-channa-punctatus-bloch-1793
#14
Arun Ratn, Yashika Awasthi, Manoj Kumar, Sunil Kumar Singh, Renu Tripathi, Sunil P Trivedi
The present study was conducted to assess the in-vivo activities of certain molecular biomarkers under the impact of phorate exposure. Fish, Channa punctatus (35 ± 3.0 g; 14.5 ± 1.0 cm; Actinopterygii) were subjected to semi-static conditions having 5% (0.0375 mg/L for T1 group) and 10% of 96 h-LC50 (0.075 mg/L for T2 group) of phorate exposure for 15 and 30 d. The oxidative stress was assessed in terms of superoxide dismutase (SOD) and catalase (CAT) activities. DNA damage was measured as induction of micronuclei (MN) and consequent differential expression of apoptotic genes-tumor suppressor (p53), apoptotic peptidase activating factor-1 (apaf-1) and catalase (cat) in liver and kidney, two major sites of biotransformation in fish, were quantified...
May 3, 2017: Chemosphere
https://www.readbyqxmd.com/read/28505574/the-effect-of-insecticides-chlorpyrifos-%C3%AE-cypermethrin-and-imidacloprid-on-primary-dna-damage-tp-53-and-c-myc-structural-integrity-by-comet-fish-assay
#15
Davor Zeljezic, Benjamin Vinkovic, Vilena Kasuba, Nevenka Kopjar, Mirta Milic, Marin Mladinic
In parallel with the continuous use of conventional insecticides, introduction of more environmentally friendly substances continues to grow in modern agriculture. In the present study, we evaluated chlorpyrifos, and imidacloprid and α-cypermethrin as two representatives of green insecticides for their genotoxic activity. We conducted a 14-day treatment in extended human lymphocytes cultures using real life exposure relevant concentrations. An alkaline comet assay was used to detect primary DNA damage. Simultaneously, the effect on the specific action towards the TP 53 and c-Myc genes in terms of fragmentation and copy number were determined...
May 3, 2017: Chemosphere
https://www.readbyqxmd.com/read/28494285/effects-of-in-situ-exposure-to-tritiated-natural-environments-a-multi-biomarker-approach-using-the-fathead-minnow-pimephales-promelas
#16
B Gagnaire, C Adam-Guillermin, A Festarini, I Cavalié, C Della-Vedova, C Shultz, S B Kim, H Ikert, C Dubois, S Walsh, F Farrow, D Beaton, E Tan, K Wen, M Stuart
Aquatic ecosystems are chronically exposed to radionuclides as well as other pollutants. Increased concentrations of pollutants in aquatic environments can present a risk to exposed organisms, including fish. The goal of this study was to characterize the effects of tritium, in the context of natural environments, on the health of fathead minnow, Pimephales promelas. Fish were exposed to tritium (activity concentrations ranging from 2 to 23,000Bq/L) and also to various concentrations of several metals to replicate multiple-stressor environments...
December 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28490783/multiple-biomarker-responses-serum-biochemistry-oxidative-stress-genotoxicity-and-histopathology-in-channa-punctatus-exposed-to-heavy-metal-loaded-waste-water
#17
Mehjbeen Javed, Md Irshad Ahmad, Nazura Usmani, Masood Ahmad
Experiments were conducted to investigate the health of fish Channa punctatus inhabiting heavy metal-loaded waste water. Heavy metals in the order of Fe > Mn > Zn > Co > Ni > Cu = Cr were present in the waste water. Gills had high metal load followed by liver and then kidney. Albumin, albumin to globulin (A:G) ratio, triglyceride, high density lipoprotein (HDL) and very low density lipoprotein (VLDL) were found to be lower but phospholipid, low density lipoprotein (LDL), total protein, lipid and cholesterol were higher as compared to the reference...
May 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28486571/ras-oncogene-and-hypoxia-inducible-factor-1-alpha-hif-1%C3%AE-expression-in-the-amazon-fish-colossoma-macropomum-cuvier-1818-exposed-to-benzo-a-pyrene
#18
Grazyelle Sebrenski da Silva, Luciana Mara Lopes Fé, Maria de Nazaré Paula da Silva, Vera Maria Fonseca de Almeida E Val
Benzo[a]pyrene (B[a]P) is a petroleum derivative capable of inducing cancer in human and animals. In this work, under laboratory conditions, we analyzed the responses of Colossoma macropomum to B[a]P acute exposure through intraperitoneal injection of four different B[a]P concentrations (4, 8, 16 and 32 μmol/kg) or corn oil (control group). We analyzed expression of the ras oncogene and the Hypoxia-inducible factor-1 alpha (hif-1α) gene using quantitative real-time PCR. Additionally, liver histopathological changes and genotoxic effects were evaluated through the comet assay...
May 8, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28482225/immunostaining-of-uva-induced-dna-damage-in-erythrocytes-of-medaka-oryzias-latipes
#19
Alaa El-Din Hamid Sayed, Hiroshi Mitani
Some authors have recently reported that UVA induces double-strand breaks (DSBs) in DNA. Only a few researchers have reported on the induction of DSBs upon UVA exposure, as measured using the Comet assay and γ-H2AX as markers of DSB formation. In the present study, we have investigated for the first time the dose-dependent induction of DSBs by UVA in medaka (Oryzias latipes) erythrocytes. Adult female medaka fish were exposed to UVA for 15, 30, and 60min/day for three continuous days; an unirradiated control group was kept in the same laboratory conditions...
June 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28437728/is-a-a-o-process-effective-in-toxicity-removal-case-study-with-coking-wastewater
#20
Liu Shi, Dong Wang, Di Cao, Chunhong Na, Xie Quan, Ying Zhang
The anaerobic-anoxic-oxic (A/A/O) process is the commonly used biological wastewater treatment process, especially for the coking wastewater. However, limit is known about its ability in bio-toxicity removal from wastewater. In this study, we evaluated the performance of A/A/O process in bio-toxicity removal from the coking wastewater, using two test species (i.e. crustacean (Daphnia magna) and zebra fish (Danio rerio)) in respect of acute toxicity, oxidative damage and genotoxicity. Our results showed that the acute toxicity of raw influent was reduced gradually along with A/A/O process and the effluent presented no acute toxicity to Daphnia magna (D...
August 2017: Ecotoxicology and Environmental Safety
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