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Amanda Bonalume Cordeiro de Morais, Carmen Alicia Daza Bolaños, Ana Carolina Alves, Cássia Yumi Ikuta, Gustavo Henrique Batista Lara, Marcos Bryan Heinemann, Rogério Giuffrida, Fernando Paganini Listoni, Mateus de Souza Ribeiro Mioni, Rodrigo Garcia Motta, Shinji Takai, Márcio Garcia Ribeiro
Mycobacterium species and the virulence-associated proteins (vapA, vapB, and vapN genes) of Rhodococcus equi isolated from 330 lymph nodes of collared peccaries (Tayassu tajacu) and white-lipped peccaries (Tayassu pecari) intended for human consumption were investigated. Thirty-six (10.9%) R. equi strains were isolated; 3.3% (n = 11/330) were from white-lipped peccary lymph nodes, and 7.6% (25/330) were from collared peccary lymph nodes. Among the 11 isolates of R. equi from the white-lipped peccaries, 90...
March 15, 2018: Tropical Animal Health and Production
M P De Cesaro, J T Dos Santos, J G Ferst, J E Nóbrega, Pra Rosa, M T Rovani, G F Ilha, R C Bohrer, R Ferreira, B G Gasperin, V Bordignon, Pbd Gonçalves
Natriuretic peptides (NPs) are known to regulate reproductive events in polyovulatory species, but their function and regulation in monovulatory species remain to be fully characterized. Using a well-established in vivo model, we found that bovine granulosa cells from follicles near the deviation stage express mRNA for the three NP receptors (NPR1, NPR2 and NPR3), but not for NP precursors (NPPA, NPPB and NPPC). The abundance of NPR3 mRNA was higher in dominant compared to subordinate follicles at the expected time of follicular deviation...
March 14, 2018: Reproduction in Domestic Animals, Zuchthygiene
Silvia Bettini, Emanuel Bordigato, Roberto Fabris, Roberto Serra, Chiara Dal Pra', Anna Belligoli, Marta Sanna, Chiara Compagnin, Mirto Foletto, Luca Prevedello, Paola Fioretto, Roberto Vettor, Luca Busetto
OBJECTIVES: Resting energy expenditure (REE) declines more than what is expected according to body composition changes after caloric restriction. This metabolic adaptation is considered one of the factors favoring weight regain. The aim of this study is to evaluate the changes of REE and calculate the degree of metabolic adaptation occurring after laparoscopic sleeve gastrectomy (LSG). METHODS: REE (by indirect calorimetry) and body composition (fat-free mass or FFM, fat mass or FM by bioelectrical impedance analysis) were determined before and after 12 months in 154 patients with obesity treated with laparoscopic sleeve gastrectomy (LSG)...
March 12, 2018: Obesity Surgery
Mark D Aupperlee, Anastasia Kariagina, Nicole Zaremba, Marc D Basson, Richard C Schwartz, Sandra Z Haslam
Increased proliferation and breast cancer risk has been observed in postmenopausal women receiving estrogen (E) + progestin hormone replacement therapy (HRT). Progestin action is mediated through two progesterone receptor (PR) isoforms, PRA and PRB, with unique transcriptional activity and function. The current study examines hormonal regulation of PR isoforms in the normal postmenopausal human breast and the mechanism by which progestins increase proliferation and breast cancer risk. Archival benign breast biopsies from postmenopausal and premenopausal women, and luminal breast tumor biopsies from postmenopausal women, were analyzed for regulation of PRA and PRB expression by E and E+medroxyprogesterone acetate (MPA)...
March 7, 2018: Translational Oncology
Gerhard Selhorst, Fabian Schmidtler, Armin Ott, Evelyn Hitzke, June Tomelden, Diethmar Antoni, Ellen Hoffmann, Johannes Rieber
Dual antiplatelet therapy (DAPT) with aspirin and a P2Y12 inhibitor is a mainstay of the prevention of stent thrombosis following percutaneous coronary intervention (PCI). In the 2015 European guidelines for the management of acute coronary syndrome (ACS), prasugrel (PRA) and ticagrelor (TICA) combined with aspirin are recommended as first-line therapy. Clopidogrel (CLO) is recommended as an alternative medication for patients with contradictions to these new drugs. This single-center study analyzed the platelet function of 809 ACS patients undergoing PCI and treatment with DAPT...
March 9, 2018: Platelets
Claudia Andrea Tortone, MartIn José Zumárraga, Andrea Karina Gioffr, Delia Susana Oriani
Background: The environment is the nontuberculous mycobacteria (NTM) reservoir, opportunistic pathogens of great diversity and ubiquity, which is observed in the constant description of new species capable of causing infection. Since its introduction, molecular methods are essential for species identification. Most comparative studies between molecular and conventional methods, have used isolated strains from clinical samples. Methods: The aim of this study was to evaluate the usefulness of molecular methods, especially the hsp65-PRA (PCR-Restriction Enzyme Analysis), and biochemical tests in the identification of NTM recovered from water of different origins, using the sequencing of 16S rRNA and hsp 65 genes as assessment methods of the previous ones...
January 2018: International Journal of Mycobacteriology
Grigoris V Karamasis, Andreas S Kalogeropoulos, Shah H Mohdnazri, Firas Al-Janabi, Rohan Jagathesan, Gerald J Clesham, Kare H Tang, Paul A Kelly, John R Davies, Thomas R Keeble
BACKGROUND: The aim of this study was to assess the impact of right atrial pressure (Pra) on non-CTO vessels FFR measurements in patients with a chronic total occlusion. METHODS: Consecutive patients who underwent PCI for a CTO of the right coronary artery (RCA) were included. Prior to RCA recanalization, FFR and FFRmyo were measured in non-CTO vessels. FFR was calculated using the Pd/Pa equation during maximum hyperaemia and also accounting for right atrial pressure (Pd-Pra/Pa-Pra)...
February 7, 2018: Cardiovascular Revascularization Medicine: Including Molecular Interventions
Mia Fahlén, Hua Zhang, Lars Löfgren, Britt Masironi, Eva VON Schoultz, B O VON Schoultz, Lena Sahlin
BACKGROUND: Progesterone and androgens are important for normal development and tumorigenesis of the breast. PATIENTS AND METHODS: Breast tissue samples from 49 premenopausal women were obtained. The progesterone receptors (PRA, PRB, PGRMC1 and PGRMC2) and the androgen receptor (AR) were determined in malignant and benign breast tumors and control tissues. RESULTS: The PRB and AR mRNA levels were highest in tumors. PGRMC1 and PGRMC2 mRNA levels were higher in malignant tumors compared to their paired normal tissues...
March 2018: Anticancer Research
Matteo Dal Prà, Davide Carta, Gyorgy Szabadkai, Matteo Suman, Yahima Frión-Herrera, Nicola Paccagnella, Giulia Castellani, Sara De Martin, Maria Grazia Ferlin
Designing novel inverse agonists of NR RORγt still represents a challenge for the pharmaceutical community to develop therapeutics for treating immune diseases. By exploring the structure of NRs natural ligands, the representative arotenoid ligands and RORs specific ligands share some chemical homologies which can be exploited to design a novel molecular structure characterized by a polycyclic core bearing a polar head and a hydrophobic tail. Compound MG 2778 (8), a cyclopenta[a]phenantrene derivative, was identified as lead compound which was chemically modified at position 2 in order to obtain a small library for preliminary SARs...
February 15, 2018: Bioorganic & Medicinal Chemistry
Jing Huai, Zi Yang, Yan-Hong Yi, Guang-Jiao Wang
Background: Pravastatin (Pra) exerts protective effects on preeclampsia. Preeclampsia is a multifactorial and pathogenic pathway syndrome. The present study compared the effects of Pra on clinical manifestations of preeclampsia in different pathogenic pathways. Methods: Two different preeclampsia-like mouse models used in this study were generated with Nω-nitro-L-arginine methyl ester (L-NAME) and used lipopolysaccharide (LPS) from day 7 of gestation, respectively...
February 20, 2018: Chinese Medical Journal
Raphael Henrique Déa Cirino, Rosana Herminia Scola, Renata Dal-Prá Ducci, Ana Cristina Camarozano Wermelinger, Claudia Suemi Kamoi Kay, Paulo José Lorenzoni, Lineu Cesar Werneck, Eliane Ribeiro Carmes, Claudio Leinig Pereira da Cunha
INTRODUCTION: Early detection of left ventricular systolic dysfunction (LVSD) is important for therapeutic strategies for Duchenne muscular dystrophy (DMD) patients. We analyzed the myocardial strain by echocardiography for the early detection of LVSD and determined the predictors of early LVSD. METHODS: Cross-sectional study of forty DMD patients with normal left ventricular ejection fraction. Global longitudinal strain (GLS) was used to analyse subtle disturbances in the longitudinal contraction of the myocardium...
February 14, 2018: Muscle & Nerve
Courtenay M Holscher, Kyle Jackson, Eric Kh Chow, Alvin G Thomas, Christine E Haugen, Sandra R DiBrito, Carlin Purcell, Matthew Ronin, Amy D Waterman, Jacqueline Garonzik Wang, Allan B Massie, Sommer E Gentry, Dorry L Segev
Kidney paired donation (KPD) can facilitate living donor transplantation for candidates with an incompatible donor, but requires waiting for a match while suffering the morbidity of dialysis. The balance between waiting for KPD versus desensitization or deceased donor transplantation relies on the ability to estimate KPD wait times. We studied donor/candidate pairs in the National Kidney Registry (NKR), a large multi-center KPD clearinghouse, between 10/2011-9/2015 using a competing risk framework. Among 1894 candidates, 52% were male, median age was 50 years, 66% were white, 59% had blood type O, 42% had PRA>80, and 50% obtained KPD through NKR...
February 13, 2018: American Journal of Transplantation
Hari Singhal, Marianne E Greene, Allison L Zarnke, Muriel Laine, Rose Al Abosy, Ya-Fang Chang, Anna G Dembo, Kelly Schoenfelt, Raga Vadhi, Xintao Qiu, Prakash Rao, Bindu Santhamma, Hareesh B Nair, Klaus J Nickisch, Henry W Long, Lev Becker, Myles Brown, Geoffrey L Greene
Major roadblocks to developing effective progesterone receptor (PR)-targeted therapies in breast cancer include the lack of highly-specific PR modulators, a poor understanding of the pro- or anti-tumorigenic networks for PR isoforms and ligands, and an incomplete understanding of the cross talk between PR and estrogen receptor (ER) signaling. Through genomic analyses of xenografts treated with various clinically-relevant ER and PR-targeting drugs, we describe how the activation or inhibition of PR differentially reprograms estrogen signaling, resulting in the segregation of transcriptomes into separate PR agonist and antagonist-mediated groups...
January 12, 2018: Oncotarget
Georgios N Kalambokis, Ilias Tsiakas, Maria Christaki, Christina Koustousi, Leonidas Christou, Gerasimos Baltayiannis, Dimitrios Christodoulou
BACKGROUND: The aim of this study was to predict the occurrence of hepatorenal syndrome (HRS) and death in patients with advanced cirrhosis and ascites. PATIENTS AND METHODS: We retrospectively evaluated 2-year data of 78 patients with cirrhosis and ascites (Child-Pugh B/C: 45/43). The mean arterial pressure (MAP) and cardiac output (CO) were measured in all patients just before administration of 2 mg of terlipressin and 30 min later. Systemic vascular resistance (SVR) was calculated as MAP/CO...
February 9, 2018: European Journal of Gastroenterology & Hepatology
Cord Sunderkötter, Karsten Becker, Heinz Kutzner, Thomas Meyer, Norbert Blödorn-Schlicht, Udo Reischl, Pietro Nenoff, Walter Geißdörfer, Yvonne Gräser, Mathias Herrmann, Joachim Kühn, Christian Bogdan
Nukleinsäure-Amplifikations-Techniken (NAT), wie die PCR, sind hochsensitiv sowie selektiv und stellen in der mikrobiologischen Diagnostik wertvolle Ergänzungen zur kulturellen Anzucht und Serologie dar. Sie bergen aber gerade bei formalinfixiertem und in Paraffin eingebettetem Gewebe ein Risiko für sowohl falsch negative als auch falsch positive Resultate, welches nicht immer richtig eingeschätzt wird. Daher haben Vertreter der Deutschen Gesellschaft für Hygiene und Mikrobiologie (DGHM) und der Deutschen Dermatologischen Gesellschaft (DDG) einen Konsensus in Form einer Übersichtsarbeit erarbeitet, wann eine NAT am Paraffinschnitt angezeigt und sinnvoll ist und welche Punkte dabei in der Präanalytik und Befundinterpretation beachtet werden müssen...
February 2018: Journal der Deutschen Dermatologischen Gesellschaft, Journal of the German Society of Dermatology: JDDG
J Luis Cortez-Sánchez, Elías Cortés-Acosta, V Mónica Cueto-Hernández, Elba Reyes-Maldonado, César Hernández-Rodríguez, Lourdes Villa-Tanaca, J Antonio Ibarra
Candida glabrata is an emerging opportunistic pathogen that has intrinsic resistance to azoles antifungals. During infection or while living as a commensal it encounters nutritional stresses such as deficiency of carbon or nitrogen sources. Herein we investigate the expression and activity of PrA, Ape1, Ape3 and CpY vacuolar proteases during these stressful nutrimental conditions. Our findings demonstrate a differential activity profile depending on the addition or lack of carbon, nitrogen or both. Of the four proteases tested, PrA and Ape3 showed a higher activity in the absence of nitrogen...
January 29, 2018: FEMS Yeast Research
Piyanat Teinlek, Kannika Siripattarapravat, Chanin Tirawattanawanich
Objective: Complete mtDNA D-loop sequences of four Thai indigenous chicken varieties, including Pra-dhu-hang-dam (PD), Leung-hang-khao (LK), Chee (CH) and Dang (DA) were explored for genetic diversity and relationships with their potential ancestor and possible associates so to address chicken domestication in Thailand. Methods: A total of 220 complete mtDNA D-loop sequences of the 4 Thai indigenous chicken varieties were obtained by Sanger direct sequencing of PCR amplicons of 1231-1232 base pair in size...
January 26, 2018: Asian-Australasian Journal of Animal Sciences
Penelope Gray, Johanna Palmroth, Tapio Luostarinen, Dan Apter, Gary Dubin, Geoff Garnett, Tiina Eriksson, Kari Natunen, Marko Merikukka, Ville Pimenoff, Anna Söderlund-Strand, Simopekka Vänskä, Jorma Paavonen, Eero Pukkala, Joakim Dillner, Matti Lehtinen
Efficacy of human papillomavirus (HPV) vaccines promises to control HPV infections. However, HPV vaccination program may lay bare an ecological niche for non-vaccine HPV types. We evaluated type-replacement by HPV type and vaccination strategy in a community-randomized trial executed in HPV vaccination naïve population. Thirty-three communities were randomized to gender-neutral vaccination with AS04-adjuvanted HPV16/18 vaccine (Arm A), HPV vaccination of girls and hepatitis B-virus (HBV) vaccination of boys (Arm B), and gender-neutral HBV vaccination (Arm C)...
January 29, 2018: International Journal of Cancer. Journal International du Cancer
Linn C Dobrowolski, Daan W Eeftinck Schattenkerk, C T Paul Krediet, Peter M Van Brussel, Liffert Vogt, Frederike J Bemelman, Jim A Reekers, Bert-Jan H Van Den Born, Hein J Verberne
BACKGROUND: Catheter-based renal sympathetic denervation (RDN) has been considered a potential treatment for therapy resistant hypertension (RHT). However, in a randomized placebo-controlled trial, RDN did not lead to a substantial blood pressure (BP) reduction. We hypothesized that variation in the reported RDN efficacy might be explained by incomplete nerve disruption as assessed by renal 123I-meta-iodobenzylguanidine (123I-mIBG) scintigraphy. METHODS: In 21 RHT patients (median age 60 years), we performed 123I-mIBG scintigraphy before and 6 weeks after RDN...
January 26, 2018: EJNMMI Research
Ying Huang, Yuan Qi, JianQing Du, DaiFu Zhang
Objectives: Protosappanin A (PrA) is an effective and major ingredient of Caesalpinia sappan L. The current study was aimed to explore the effect of PrA on atherosclerosis (AS). Materials and Methods: Firstly, the experimental model of AS was established in rabbits by two-month feeding of high fat diet. Then, the rabbits were randomly divided into five groups and treated with continuous high lipid diet (model control), high lipid diet containing rosuvastatin (positive control), 5 mg/kg PrA (low dose) or 25 mg/kg PrA (high dose)...
January 2018: Iranian Journal of Basic Medical Sciences
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