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Donor specific antibodies

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https://www.readbyqxmd.com/read/29932259/porcine-endothelial-grafts-could-survive-for-a-long-term-without-using-systemic-immunosuppressors-an-investigation-of-feasibility-and-efficacy-of-xeno-descemet-s-stripping-automated-endothelial-keratoplasty-from-wzs-pig-to-rhesus-monkey
#1
Yang Liu, YingNan Zhang, Qingfeng Liang, Chao Yan, Li Wang, Jing Zhang, ZhiQiang Pan
BACKGROUND: In current non-human primate models, full-thickness porcine grafts could not achieve long-term survival without using potent systemic immunosuppressors. Moreover, the thickness disparity in xeno-PKP proved to be hard to manipulate and may cause several complications which also could prevent the grafts from long survival. Considering the advantages of Descemet's stripping automated endothelial keratoplasty (DSAEK) derived from its ultrathin graft and lower rejection rate, we hypothesize xeno-DSAEK may overcome these imperfections in xeno-PKP...
June 22, 2018: Xenotransplantation
https://www.readbyqxmd.com/read/29929009/safety-immunogenicity-and-protection-of-a-h3n2-live-attenuated-influenza-vaccines-containing-wild-type-nucleoprotein-in-a-ferret-model
#2
Daniil A Korenkov, Karen L Laurie, Patrick C Reading, Louise A Carolan, Kok Fei Chan, Irina I Isakova-Sivak, Tatiana A Smolonogina, Kanta Subbarao, Ian G Barr, Julie Villanueva, Svetlana Shcherbik, Tatiana Bousse, Larisa G Rudenko
Live attenuated influenza vaccines (LAIVs) are promising tools for the induction of broad protection from influenza due to their ability to stimulate cross-reactive T cells against influenza pathogens. One of the major targets for cytotoxic T-cell immunity is viral nucleoprotein (NP), which is relatively conserved among antigenically distant influenza viruses. Nevertheless, a diversity of epitope composition has been found in the NP protein of different lineages of influenza A viruses. The H2N2 master donor virus which is currently used as a backbone for the LAIV and donor of the six genomic segments encoding the internal proteins, A/Leningrad/134/17/57 (MDV Len/17), was isolated 60 years ago...
June 18, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29928274/sialylated-autoantigen-reactive-igg-antibodies-attenuate-disease-development-in-autoimmune-mouse-models-of-lupus-nephritis-and-rheumatoid-arthritis
#3
Yannic C Bartsch, Johann Rahmöller, Maria M M Mertes, Susanne Eiglmeier, Felix K M Lorenz, Alexander D Stoehr, Dominique Braumann, Alexandra K Lorenz, André Winkler, Gina-Maria Lilienthal, Janina Petry, Juliane Hobusch, Moritz Steinhaus, Constanze Hess, Vivien Holecska, Carolin T Schoen, Carolin M Oefner, Alexei Leliavski, Véronique Blanchard, Marc Ehlers
Pro- and anti-inflammatory effector functions of IgG antibodies (Abs) depend on their subclass and Fc glycosylation pattern. Accumulation of non-galactosylated (agalactosylated; G0) IgG Abs in the serum of rheumatoid arthritis and systemic lupus erythematosus (SLE) patients reflects severity of the diseases. In contrast, sialylated IgG Abs are responsible for anti-inflammatory effects of the intravenous immunoglobulin (pooled human serum IgG from healthy donors), administered in high doses (2 g/kg) to treat autoimmune patients...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29921651/safety-and-efficacy-of-intracoronary-infusion-of-allogeneic-human-cardiac-stem-cells-in-patients-with-st-segment-elevation-myocardial-infarction-and-left-ventricular-dysfunction-a-multicenter-randomized-double-blind-and-placebo-controlled-clinical-trial
#4
Francisco Fernández-Avilés, Ricardo Sanz-Ruiz, Jan Bogaert, Ana Casado Plasencia, Inmaculada Gilaberte, Ann Belmans, María Eugenia Fernández-Santos, Dominique Charron, Miguel Mulet, Raquel Yotti, Itziar Palacios, Manuel Luque, Rafael Sádaba, J A San Roman, Mariano Larman, Pedro L Sánchez, Juan Sanchis, Manuel F Jimenez-Navarro, Piet Claus, Reem Al-Daccak, Eleuterio Lombardo, José Luis Abad, Olga Delarosa, Lucia Corcostegui, Javier Bermejo, Stefan Janssens
<u>Rationale:</u> Allogeneic cardiac stem cells (AlloCSC-01) have shown protective, immunoregulatory and regenerative properties with a robust safety profile in large animal models of heart disease. <u>Objective:</u> To investigate the safety and feasibility of early administration of AlloCSC-01 in patients with ST-segment elevation myocardial infarction (STEMI). <u>Methods and Results:</u> CAREMI was a phase I/II multicenter, randomized, double-blind, placebo-controlled trial in patients with STEMI, LVEF ≤ 45% and infarct size ≥ 25% of left ventricular (LV) mass by cardiac magnetic resonance (MR), who were randomized (2:1) to receive AlloCSC-01 or placebo through the intracoronary route at day 5-7...
June 19, 2018: Circulation Research
https://www.readbyqxmd.com/read/29920186/th1-th17-polarization-persists-following-whole-cell-pertussis-vaccination-despite-repeated-acellular-boosters
#5
Ricardo da Silva Antunes, Mariana Babor, Chelsea Carpenter, Natalie Khalil, Mario Cortese, Alexander J Mentzer, Grégory Seumois, Christopher D Petro, Lisa A Purcell, Pandurangan Vijayanand, Shane Crotty, Bali Pulendran, Bjorn Peters, Alessandro Sette
In the mid-1990s, whole-cell (wP) pertussis vaccines were associated with local and systemic adverse events, which prompted their replacement with acellular (aP) vaccines in many high-income countries. In the past decade rates of pertussis disease have increased in children receiving only acellular pertussis vaccines. We compared the immune responses to acellular pertussis boosters in children who received their initial doses with either wP or aP vaccines using activation-induced marker (AIM) assays. Specifically, we examined pertussis-specific memory CD4+ T cell responses ex vivo, highlighting a Type 2/Th2 versus Type 1/Th1 and Th17 differential polarization as a function of childhood vaccination...
June 19, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29916988/understanding-the-correlation-between-dsa-complement-activation-and-antibody-mediated-rejection-in-heart-transplant-recipients
#6
Qiuheng Zhang, Michelle Hickey, Diana Drogalis-Kim, Ying Zheng, David Gjertson, Martin Cadeiras, Tam Khuu, Arnold S Baas, Eugene C Depasquale, Nancy J Halnon, Gregory Perens, Juan Alejos, Daniel Cruz, Nsair Ali, Richard Shemin, Murray Kwon, Michael C Fishbein, Abbas Ardehali, Mario Deng, Elaine F Reed
BACKGROUND: Donor specific HLA antibodies (DSA) are associated with increased rates of rejection and of graft failure in cardiac transplantation. The goal of this study was to determine the association of preformed and posttransplant development of newly detected DSA (ndDSA) with antibody mediated rejection (AMR) and characterize the clinical relevance of complement activating DSA in heart allograft recipients. METHODS: The study included 128 adult and 48 pediatric heart transplant patients transplanted between 2010 to 2013...
June 18, 2018: Transplantation
https://www.readbyqxmd.com/read/29915589/immunoglobulin-g-fragment-crystallizable-glycosylation-after-hematopoietic-stem-cell-transplantation-is-dissimilar-to-donor-profiles
#7
Noortje de Haan, Maarten J D van Tol, Gertjan J Driessen, Manfred Wuhrer, Arjan C Lankester
Immunoglobulin G (IgG) fragment crystallizable (Fc) N -glycosylation has a large influence on the affinity of the antibody for binding to Fcγ-receptors (FcγRs) and C1q protein, thereby influencing immune effector functions. IgG Fc glycosylation is known to be partly regulated by genetics and partly by stimuli in the microenvironment of the B cell. Following allogeneic hematopoietic stem cell transplantation (HSCT), and in the presence of (almost) complete donor chimerism, IgG is expected to be produced by, and glycosylated in, B cells of donor origin...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29915167/outcomes-of-induction-therapy-with-rabbit-anti-thymocyte-globulin-in-heart-transplant-recipients-a-single-center-retrospective-cohort-study
#8
Tambi Jarmi, Nirav Patel, Sadaf Aslam, George Makdisi, Elias Doumit, Rahul Mhaskar, Branko Miladinovic, Mark Weston
BACKGROUND Induction immunosuppression is used in transplantation to prevent early acute rejection. The survival benefit of rabbit anti-thymocyte globulin (rATG) induction has not been established yet. We sought to determine the role of rATG in preventing rejection and improving overall survival. MATERIAL AND METHODS A retrospective cohort study was conducted from 2005 to 2009 and data of consecutive 268 heart transplant recipients were reviewed. RESULTS The data of 144 patients who received induction with rATG were compared to 124 patients who did not...
June 19, 2018: Annals of Transplantation: Quarterly of the Polish Transplantation Society
https://www.readbyqxmd.com/read/29913037/does-human-leukocyte-antigens-sensitization-matter-for-xenotransplantation
#9
REVIEW
Guerard W Byrne
The major histocompatibility complex class I and class II human leukocyte antigens (HLA) play a central role in adaptive immunity but are also the dominant polymorphic proteins targeted in allograft rejection. Sensitized patients with high levels of panel-reactive anti-HLA antibody (PRA) are at risk of early allograft injury, rejection, reduced allograft survival and often experience prolonged waiting times prior to transplantation. Xenotransplantation, using genetically modified porcine organs, offers a unique source of donor organs for these highly sensitized patients if the anti-HLA antibody, which places the allograft at risk, does not also enhance anti-pig antibody reactivity responsible for xenograft rejection...
May 2018: Xenotransplantation
https://www.readbyqxmd.com/read/29912041/cincinnati-protocol-for-preoperative-screening-and-donor-selection-for-ocular-surface-stem-cell-transplantation
#10
Albert Y Cheung, Enrica Sarnicola, Khaliq H Kurji, Amit Govil, Gautham Mogilishetty, Medi Eslani, Elizabeth Wright, Paul Brailey, Edward J Holland
PURPOSE: To describe our process for preoperative screening and donor selection for ocular surface stem cell transplantation (OSST). METHODS: A 7-year retrospective chart review was performed on limbal stem cell deficiency patients. The inclusion criterion was all patients who underwent an OSST procedure. The exclusion criterion was eyes with unilateral disease in which an autograft was performed. Data for human leukocyte antigen (HLA) typing, virtual crossmatching, donor-specific antibody, and panel reactive antibody level were obtained...
June 14, 2018: Cornea
https://www.readbyqxmd.com/read/29908316/the-clinical-significance-of-epitope-mismatch-load-in-kidney-transplantation-a-multicentre-study
#11
Liesbeth Daniëls, Maarten Naesens, Jean-Louis Bosmans, Daniel Abramowicz, Evi Nagler, Steven Van Laecke, Patrick Peeters, Dirk Kuypers, Marie-Paule Emonds
Since the advent of kidney transplantation a key strategy for maximising graft survival by avoiding allorecognition has been to minimise HLA mismatching between donor and recipient. As HLA antibodies are now recognised as being specific for epitopes and donor-recipient HLA mismatch at the amino acid level can now be determined, HLA epitope mismatch load could be a better predictor for dnDSA development than classical HLA antigen mismatch calculation. This hypothesis has been investigated by other studies and the aim of our multicentre study was to confirm this observation in our population...
June 13, 2018: Transplant Immunology
https://www.readbyqxmd.com/read/29904381/the-calcineurin-inhibitor-tacrolimus-specifically-suppresses-human-t-follicular-helper-cells
#12
Elizabeth F Wallin, Danika L Hill, Michelle A Linterman, Kathryn J Wood
Background: T follicular helper (Tfh) cells are key players in the production of antibody-producing B cells via the germinal center reaction. Therapeutic strategies targeting Tfh cells are important where antibody formation is implicated in disease, such as transplant rejection and autoimmune diseases. We investigated the impact of the immunosuppressive agent tacrolimus on human Tfh cell differentiation and function in transplant recipients. Methods: Paired blood and lymph node (LN) samples were obtained from 61 transplant recipients immediately prior to organ implantation...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29902635/overcoming-non-specific-binding-to-measure-the-active-concentration-and-kinetics-of-serum-anti-hla-antibodies-by-surface-plasmon-resonance
#13
Jonathan Visentin, Lionel Couzi, Claire Dromer, Martine Neau-Cransac, Gwendaline Guidicelli, Vincent Veniard, Karine Nubret-le Coniat, Pierre Merville, Carmelo Di Primo, Jean-Luc Taupin
Human leukocyte antigen (HLA) donor-specific antibodies are key serum biomarkers for assessing the outcome of transplanted patients. Measuring their active concentration, i.e. the fraction that really interacts with donor HLA, and their affinity could help deciphering their pathogenicity. Surface plasmon resonance (SPR) is recognized as the gold-standard for measuring binding kinetics but also active concentrations, without calibration curves. SPR-based biosensors often suffer from non-specific binding (NSB) occurring with the sensor chip surface and the immobilized targets, especially for complex media such as human serum...
June 7, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29898396/co-evolution-of-hiv-envelope-and-apex-targeting-neutralizing-antibody-lineage-provides-benchmarks-for-vaccine-design
#14
Kimmo Rantalainen, Zachary T Berndsen, Sasha Murrell, Liwei Cao, Oluwarotimi Omorodion, Jonathan L Torres, Mengyu Wu, Jeffrey Umotoy, Jeffrey Copps, Pascal Poignard, Elise Landais, James C Paulson, Ian A Wilson, Andrew B Ward
Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop. Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system. Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort. Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes...
June 12, 2018: Cell Reports
https://www.readbyqxmd.com/read/29890181/prevalence-and-risk-factors-of-antibodies-to-human-leukocyte-antigens-in-haploidentical-stem-cell-transplantation-candidates-a-multi-center-study
#15
Ming-Rui Huo, Ya-Jing Xu, Shu-Zhen Zhai, Meng Lv, Yu Wang, Le-Qing Cao, Lan-Ping Xu, Xiao-Hui Zhang, Huan Chen, Yu-Hong Chen, Feng-Rong Wang, Wei Han, Yu-Qian Sun, Chen-Hua Yan, Fei-Fei Tang, Xiao-Dong Mo, Ming-Feng Zhao, Kai-Yan Liu, Xiao-Jun Huang, Ying-Jun Chang
We investigated the prevalence of and risk factors for antibodies to HLA in 1,663 haploidentical transplant candidates. Among these cases, 349 (21.0%) showed positive panel-reactive antibody (PRA) either for class I or class II HLA. Multivariate analysis showed the following: i) risk factors associated with the prevalence of PRA either for class I or class II HLA were female gender (P=0.018), prior transfusions (P<0.001) or pregnancy (P<0.001), and cases with MDS (P=0.018); compared to other patients, subjects with ALL had a lower prevalence of class I antibodies (P=0...
June 8, 2018: Human Immunology
https://www.readbyqxmd.com/read/29889829/humoral-and-cellular-immune-responses-to-yersinia-pestis-pla-antigen-in-humans-immunized-with-live-plague-vaccine
#16
Valentina A Feodorova, Anna M Lyapina, Maria A Khizhnyakova, Sergey S Zaitsev, Lidiya V Sayapina, Tatiana E Arseneva, Alexey L Trukhachev, Svetlana A Lebedeva, Maxim V Telepnev, Onega V Ulianova, Elena P Lyapina, Sergey S Ulyanov, Vladimir L Motin
BACKGROUND: To establish correlates of human immunity to the live plague vaccine (LPV), we analyzed parameters of cellular and antibody response to the plasminogen activator Pla of Y. pestis. This outer membrane protease is an essential virulence factor that is steadily expressed by Y. pestis. METHODOLOGY/PRINCIPAL FINDINGS: PBMCs and sera were obtained from a cohort of naïve (n = 17) and LPV-vaccinated (n = 34) donors. Anti-Pla antibodies of different classes and IgG subclasses were determined by ELISA and immunoblotting...
June 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29888557/persistence-of-de-novo-donor-specific-hla-antibodies-after-lung-transplantation-a-potential-marker-of-decreased-patient-survival
#17
M Schmitzer, H Winter, N Kneidinger, G Meimarakis, A Dick, R Schramm, L V Klotz, G Preissler, N Strobl, V von Dossow, C Schneider, T Weig, R Hatz, T Kauke
The impact of de novo donor-specific anti-HLA antibodies (DSA) on outcomes in lung transplantation is still a matter of debate. We hypothesize that differentiating DSA by persistent and transient appearance may offer an additional risk assessment. The clinical relevance of HLA-antibodies was investigated prospectively in 72 recipients with a median follow-up period of 21 months. The presence of HLA-antibodies was analysed by single antigen bead assay prior to and after (3 weeks, 3, 6, 12 and 18 months) transplantation...
June 10, 2018: HLA
https://www.readbyqxmd.com/read/29888023/absence-of-rejection-in-a-facial-allograft-recipient-with-a-positive-flow-crossmatch-24-months-after-induction-with-rabbit-anti-thymocyte-globulin-and-anti-cd20-monoclonal-antibody
#18
Bruce E Gelb, J Rodrigo Diaz-Siso, Natalie M Plana, Adam Jacoby, William J Rifkin, Kimberly S Khouri, Daniel J Ceradini, Eduardo D Rodriguez
Background: Donor-specific antibodies (DSA) to human leukocyte antigen increase the risk of accelerated rejection and allograft damage and reduce the likelihood of successful transplantation. Patients with full-thickness facial burns may benefit from facial allotransplantation. However, they are at a high risk of developing DSA due to standard features of their acute care. Case Presentation: A 41-year-old male with severe disfigurement from facial burns consented to facial allotransplantation in 2014; panel reactive antibody score was 0%...
2018: Case Reports in Transplantation
https://www.readbyqxmd.com/read/29885443/value-of-c3d-assay-and-igg-subclass-in-the-prediction-of-the-flow-cytometry-cross-match-result-for-renal-transplantation
#19
Alan G Contreras, Aurora Casillas-Abundis, Josefina Alberú, Luis Llorente, Guadalupe Lima, Adriana Arvizu, Adrián de Santiago, Mario Vilatobá, Julio Granados, Luis E Morales-Buenrostro, Rodrigo Cruz, José M Arreola-Guerra
The aim of this study is to compare the association and the predictive capacity of DSA MFI, complement fixing capacity (C3d assay) and IgG subclasses determination in the prediction of FCxM result. METHODS: We used cryopreserved (70C) sera from potential renal transplant recipients, containing DSA against their respective potential donors. All patients showed negative AHG-CDC CxM and either positive or negative FCxM. Class I and Class II HLA-DSA were determined by Luminex SAB. C3d were detected by luminex (Lifecodes®Immucor), DSA IgG 1-4 subclasses were evaluated using monoclonal antibodies specific for IgG subclasses (Luminex)...
June 6, 2018: Transplant Immunology
https://www.readbyqxmd.com/read/29880346/correlation-between-the-transplant-evaluation-rating-scale-ters-and-medical-outcomes-in-living-donor-kidney-transplant-recipients-a-retrospective-analysis
#20
G Dieplinger, N Mokhaberi, R Wahba, S Peltzer, D Buchner, H A Schlösser, V Ditt, A von Borstel, U Bauerfeind, U Lange, W Arns, C Kurschat, H A Stippel, F Vitinius
BACKGROUND: Pretransplant psychosocial evaluation of living-donor kidney transplantation (LDKT) candidates identifies recipients with potentially inferior posttransplant outcomes. Rating instruments, based on semi-standardized interviews, help to improve and standardize psychosocial evaluation. The goal of this study was to retrospectively investigate the correlation between the Transplant Evaluation Rating Scale (TERS) and transplant outcome in LDKT recipients. METHODS: TERS scores were retrospectively generated by 2 raters based on comprehensive interviews of 146 LDKT recipients conducted by mental health professionals (interrater reliability, 0...
June 2018: Transplantation Proceedings
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