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https://www.readbyqxmd.com/read/29053700/come-to-the-light-side-in-vivo-monitoring-of-pseudomonas-aeruginosa-biofilm-infections-in-chronic-wounds-in-a-diabetic-hairless-murine-model
#1
Alessandra M Agostinho Hunt, Jacob A Gibson, Casandra L Larrivee, Sandra O'Reilly, Svetlana Navitskaya, Julia V Busik, Christopher M Waters
The presence of bacteria as structured biofilms in chronic wounds, especially in diabetic patients, is thought to prevent wound healing and resolution. Chronic mouse wounds models have been used to understand the underlying interactions between the microorganisms and the host. The models developed to date rely on the use of haired animals and terminal collection of wound tissue for determination of viable bacteria. While significant insight has been gained with these models, this experimental procedure requires a large number of animals and sampling is time consuming...
October 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29053557/bacterial-keratitis-isolated-organisms-and-antibiotic-resistance-patterns-in-san-francisco
#2
Michelle Y Peng, Vicky Cevallos, Stephen D McLeod, Thomas M Lietman, Jennifer Rose-Nussbaumer
PURPOSE: Understanding the spectrum of pathogens in a given geographic region is important when deciding on empiric antibiotic therapy. In this study, we evaluate the spectrum of bacterial organisms cultured from corneal samples and their antibiotic sensitivities to guide initial treatment of keratitis. METHODS: We performed a retrospective case review of cultures from suspected infectious keratitis cases at the Francis I. Proctor Foundation, University of California, San Francisco, from 1996 through 2015...
October 17, 2017: Cornea
https://www.readbyqxmd.com/read/29053348/use-of-bacterial-acc-deaminase-to-increase-oil-especially-poly-aromatic-hydrocarbons-phytoremediation-efficiency-for-maize-zea-mays-seedlings
#3
Rezvani Borujeni Samira, Khavazi Kazem, Asgharzadeh Ahmad, Rezvani Borujeni Iraj
Oil presence in soil, as a stressor, reduces phytoremediation efficiency through an increase in the plant stress ethylene. Bacterial 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, as a plant stress ethylene reducer, was employed to increase oil phytoremediation efficiency. For this purpose, the ability of ACC deaminase-producing Pseudomonas strains to grow in oil-polluted culture media and withstand various concentrations of oil and also their ability to reduce plant stress ethylene and enhance some growth characteristics of maize and finally their effects on increasing phytoremediation efficiency of poly aromatic hydrocarbons (PAHs) in soil were investigated...
October 20, 2017: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/29053252/optogenetics-manipulation-enables-to-prevent-biofilm-formation-of-engineered-pseudomonas-aeruginosa-on-surfaces
#4
Lu Pu, Shuai Yang, Aiguo Xia, Fan Jin
Synthetic biologists have attempted to solve real-world problems, such as those of bacterial biofilms, that are involved in the pathogenesis of many clinical infections and difficult to eliminate. To address this, we employed a blue light responding system and integrated it into the chromosomes of Pseudomonas aeruginosa. With making rational adaption and improvement of the light-activated system, we provided a robust and convenient means to spatiotemporally control gene expression and manipulate biological processes with minimal perturbation in P...
October 20, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29053243/identification-of-the-biosynthetic-pathway-for-the-antibiotic-bicyclomycin
#5
Jon Patteson, Wenlong Cai, Rachel A Johnson, Kevin Santa Maria, Bo Li
Diketopiperazines (DKPs) are a large group of natural products with diverse structures and biological activities. Bicyclomycin is a broad-spectrum DKP antibiotic with unique structure and function: it contains a highly oxidized bicyclic [4.2.2] ring and is the only known selective inhibitor of the bacterial transcription termination factor, Rho. Here, we identify the biosynthetic gene cluster for bicyclomycin containing six iron-dependent oxidases. We demonstrate that the DKP core is made by a tRNA-dependent cyclodipeptide synthase, and hydroxylations on two unactivated sp3 carbons are performed by two mononuclear iron, α-ketoglutarate-dependent hydroxylases...
October 20, 2017: Biochemistry
https://www.readbyqxmd.com/read/29051980/genomic-analysis-of-bacillus-sp-strain-b25-a-biocontrol-agent-of-maize-pathogen-fusarium-verticillioides
#6
Nadia R Douriet-Gámez, Ignacio E Maldonado-Mendoza, Enrique Ibarra-Laclette, Jochen Blom, Carlos L Calderón-Vázquez
Bacillus sp. B25 is an effective biocontrol agent against the maize pathogenic fungus Fusarium verticillioides (Fv). Previous in vitro assays have shown that B25 has protease, glucanase, and chitinase activities and siderophores production; however, specific mechanisms by which B25 controls Fv are still unknown. To determine the genetic traits involved in biocontrol, B25 genome was sequenced and analyzed. B25 genome is composed of 5,113,413 bp and 5251 coding genes. A multilocus phylogenetic analysis (MLPA) suggests that B25 is closely related to the Bacillus cereus group and a high percentage (70-75%) of the genetic information is conserved between B25 and related strains, which include most of the genes associated to fungal antagonism...
October 19, 2017: Current Microbiology
https://www.readbyqxmd.com/read/29051847/symbiotic-bacteria-associated-with-puffer-fish-gastrophysus-spadiceus-and-evaluation-of-their-antimicrobial-activities
#7
Yi-Huan Wang, Nimaichand Salam, Qing Liu, Zi-Wen Yang, Li-Xiang Cao, Xiao-Lin Meng, Guo-Xing Nie, Jian-Hua Ju, Wen-Jun Li
The present study reports the diversity of culturable bacteria associated with the puffer fish Gastrophysus spadiceus. During the study, a total of 31 strains affiliated to the genera Pseudomonas, Janthinobacterium, Rahnella, and Psychrobacter were isolated from liver, intestines, and flesh of G. spadiceus. These strains exhibited a diverse range of metabolites as indicated by the HPLC and TLC profiles of the chemical extracts of their fermentation products. Some of these crude extracts showed strong antimicrobial activities against pathogenic bacterial strains...
December 2017: 3 Biotech
https://www.readbyqxmd.com/read/29051846/enhanced-biodegradation-of-mixed-pahs-by-mutated-naphthalene-1-2-dioxygenase-encoded-by-pseudomonas-putida-strain-kd6-isolated-from-petroleum-refinery-waste
#8
Kunal Dutta, Sergey Shityakov, Prangya P Das, Chandradipa Ghosh
Polycyclic aromatic hydrocarbons (PAHs) are a group of environmental pollutant that are given top priority to maintain water and soil quality to the most amenable standard. Biodegradation of PAHs by bacteria is the convenient option for decontamination on site or off site. The aim of the present study was to isolate and identify naturally occurring bacteria having mixed PAHs biodegradation ability. The newly isolated Pseudomonas putida strain KD6 was found to efficiently degrade 97.729% of 1500 mg L(-1) mixed PAHs within 12 days in carbon-deficient minimal medium (CSM)...
December 2017: 3 Biotech
https://www.readbyqxmd.com/read/29051668/culture-broth-of-ganoderma-lucidum-exhibited-antioxidant-antibacterial-and-%C3%AE-amylase-inhibitory-activities
#9
Rakrudee Sarnthima, Saranyu Khammaung, Piyawan Sa-Ard
Culture broth of Ganoderma lucidum was determined for antioxidant, antibacterial and α-amylase inhibitory activities. The culture broth contained protein as determined by Bradford method equaled to 0.2 mg/ml and total phenol content as 0.078 mg GAE/mg protein (0.557 mg GAE/g extract). It exhibited radicals scavenging activities against ABTS(+·) and DPPH(·) radicals with a half maximal inhibitory concentration (IC50) less than 1.70 ± 0.02 and 2.28 ± 0.02 µg protein/ml, respectively and reducing power equaled to 4...
October 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29051609/liquid-infused-nitric-oxide-releasing-linorel-silicone-for-decreased-fouling-thrombosis-and-infection-of-medical-devices
#10
Marcus J Goudie, Jitendra Pant, Hitesh Handa
Recent reports on liquid-infused materials have shown promise in creating ultra-low fouling surfaces, but are limited in their ability to prevent bacterial proliferation and prevent platelet activation in blood-contacting applications. In this work, a liquid-infused nitric oxide-releasing (LINORel) material is created by incorporating the nitric oxide (NO) donor S-nitroso-acetylpenicillamine (SNAP) and silicone oil in commercial medical grade silicone rubber tubing through a solvent swelling process. This combination provides several key advantages over previous NO-releasing materials, including decreased leaching of NO donor, controlled release of NO, and maintenance of ultra-low fouling property of liquid-infused materials...
October 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29051092/a-study-on-the-targeted-surveillance-of-nosocomial-infection-in-intensive-care-units-of-177-hospitals-in-jiangsu-province-of-china
#11
Yang Li, Xiao-Li Cao, Hai Ge, Yi-Hong Jiang, Hong Zhou, Wei Zheng
BACKGROUND: Nosocomial infections (NIs) impact care and costs in hospitals across the globe. There are minimal data on targeted surveillance of NI in intensive care units (ICUs), and data specific to the risk factors for NI are especially limited. METHODS: One hundred seventy-seven secondary and tertiary hospitals performed NI targeted surveillance in their ICUs. The data were collected and summarized by Minke software, then fed back once per quarter. RESULTS: The incidence of NI appeared to decrease, and the incidence of NI per 1000 patient-days and adjusted incidence were 25...
October 16, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29050883/evaluation-of-antimicrobial-potential-of-successive-extracts-of-ulmus-wallichiana-planch
#12
Kundan Singh Bora, Akhilesh Kumar, Gajraj Bisht
BACKGROUND: The plant Ulmus wallichiana Planch. is found in hills of Uttarakhand, India. Bark of U. wallichiana is commonly used as traditional healer for bone fracture of animals as well as human beings and also used as wound healer remedy. OBJECTIVE: The present study was designed to evaluate antimicrobial potential of various extracts of U. wallichiana bark. MATERIALS AND METHODS: Soxhlet extraction method was used for preparation of different extracts viz...
October 16, 2017: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/29049954/application-of-carnobacterium-maltaromaticum-as-a-feed-additive-for-weaned-rabbits-to-improve-meat-microbial-quality-and-safety
#13
Amenan Prisca Koné, Juliana Maria Velez Zea, Dominic Gagné, Dany Cinq-Mars, Frédéric Guay, Linda Saucier
This study addresses the improvement of meat microbial quality by enriching the diet of farm animals with a protective culture. Weaned Grimaud rabbits were divided into two experimental groups: a control and a diet supplemented with Micocin® (Carnobacterium maltaromaticum CB1; 8Log10CFU/kg of feed). Overall, meat quality was not affected substantially by the treatment. Total Aerobic Mesophilic (TAM), Escherichia coli and other coliforms, Enterobacteriaceae, Staphylococcus aureus, Pseudomonas spp., Listeria spp...
October 2, 2017: Meat Science
https://www.readbyqxmd.com/read/29049368/low-overlap-between-carbapenem-resistant-pseudomonas-aeruginosa-genotypes-isolated-from-hospitalized-patients-and-wastewater-treatment-plants
#14
Andrej Golle, Sandra Janezic, Maja Rupnik
The variability of carbapenem-resistant Pseudomonas aeruginosa strains (CRPA) isolated from urine and respiratory samples in a large microbiological laboratory, serving several health care settings, and from effluents of two wastewater treatment plants (WWTP) from the same region was assessed by PFGE typing and by resistance to 10 antibiotics. During the 12-month period altogether 213 carbapenem-resistant P. aeruginosa isolates were cultured and distributed into 65 pulsotypes and ten resistance profiles. For representatives of all 65 pulsotypes 49 different MLSTs were determined...
2017: PloS One
https://www.readbyqxmd.com/read/29049366/transgenic-expression-of-antimicrobial-peptide-d2a21-confers-resistance-to-diseases-incited-by-pseudomonas-syringae-pv-tabaci-and-xanthomonas-citri-but-not-candidatus-liberibacter-asiaticus
#15
Guixia Hao, Shujian Zhang, Ed Stover
Citrus Huanglongbing (HLB) associated with 'Candidatus Liberibacter asiaticus' (Las) and citrus canker disease incited by Xanthomonas citri are the most devastating citrus diseases worldwide. To control citrus HLB and canker disease, we previously screened over forty antimicrobial peptides (AMPs) in vitro for their potential application in genetic engineering. D2A21 was one of the most active AMPs against X. citri, Agrobacterium tumefaciens and Sinorhizobium meliloti with low hemolysis activity. Therefore, we conducted this work to assess transgenic expression of D2A21 peptide to achieve citrus resistant to canker and HLB...
2017: PloS One
https://www.readbyqxmd.com/read/29048607/the-arabidopsis-chromatin-remodeling-factor-chr5-regulates-plant-immune-responses-and-nucleosome-occupancy
#16
Baohong Zou, Qi Sun, Wenli Zhang, Yuan Ding, Dong-Lei Yang, Jian Hua
ATP-dependent chromatin remodeling factors use the energy of ATP hydrolysis to alter the structure of chromatin and are important regulators of eukaryotic gene expression. One such factor CHR5 (Chromatin Remodeling Factor 5) in Arabidopsis (Arabidopsis thaliana) was previously found to be involved in growth and development regulation. Here we show that CHR5 is required for the upregulation of the intracellular immune receptor gene SNC1 (SUPPRESSOR OF npr1-1, CONSTITUTIVE1) and consequently the autoimmunity induced by SNC1 upregulation...
October 17, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29048473/use-of-taurolidine-in-lung-transplantation-for-cystic-fibrosis-and-impact-on-bacterial-colonization
#17
Mohamed Zeriouh, Anton Sabashnikov, Nikhil P Patil, Bastian Schmack, Barlomiej Zych, Prashant N Mohite, Diana García Sáez, Achim Koch, Ashham Mansur, Simona Soresi, Alexander Weymann, Nandor Marczin, Thorsten Wahlers, Fabio De Robertis, André Rüdiger Simon, Aron-Frederik Popov
OBJECTIVES: The presence of bacterial colonization that causes chronic pulmonary infections in cystic fibrosis (CF) patients remains a key issue before lung transplantation. We sought to assess the impact of intraoperative taurolidine lavage on bacterial colonization and long-term outcomes following lung transplantation in CF patients. METHODS: Between 2007 and 2013, 114 CF patients underwent lung transplantation at our institute, and taurolidine 2% bronchial lavage was applied in a substantial proportion of patients (n = 42)...
October 18, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/29048369/effect-of-curcumin-on-the-diversity-of-gut-microbiota-in-ovariectomized-rats
#18
Zhiguo Zhang, Yanjing Chen, Lihua Xiang, Zhen Wang, Gary Guishan Xiao, Jingqing Hu
Curcumin has been proven to have a weight-loss effect in a menopausal rat model induced by ovariectomy. However, the effects of curcumin on gut microfloral communities of ovariectomized (OVX) rats remains unclear. Here, we used high-throughput 16S rDNA sequencing to explore the effects of curcumin on microbial diversity in the gut of OVX rats. Female Wistar rats were subjected to either ovariectomy or a sham operation (SHAM group). The OVX rats were treated with vehicle (OVX group) or curcumin (CUR group) by oral gavage...
October 19, 2017: Nutrients
https://www.readbyqxmd.com/read/29047423/antimicrobial-nonsusceptibility-of-gram-negative-bloodstream-isolates-veterans-health-administration-system-united-states-2003-2013-1
#19
Michihiko Goto, Jennifer S McDanel, Makoto M Jones, Daniel J Livorsi, Michael E Ohl, Brice F Beck, Kelly K Richardson, Bruce Alexander, Eli N Perencevich
Bacteremia caused by gram-negative bacteria is associated with serious illness and death, and emergence of antimicrobial drug resistance in these bacteria is a major concern. Using national microbiology and patient data for 2003-2013 from the US Veterans Health Administration, we characterized nonsusceptibility trends of community-acquired, community-onset; healthcare-associated, community-onset; and hospital-onset bacteremia for selected gram-negative bacteria (Escherichia coli, Klebsiella spp., Pseudomonas aeruginosa, and Acinetobacter spp...
November 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29047333/electrochemical-performance-and-microbial-community-profiles-in-microbial-fuel-cells-in-relation-to-electron-transfer-mechanisms
#20
Naroa Uria, Isabel Ferrera, Jordi Mas
BACKGROUND: Microbial fuel cells (MFCs) operating with complex microbial communities have been extensively reported in the past, and are commonly used in applications such as wastewater treatment, bioremediation or in-situ powering of environmental sensors. However, our knowledge on how the composition of the microbial community and the different types of electron transfer to the anode affect the performance of these bioelectrochemical systems is far from complete. To fill this gap of knowledge, we designed a set of three MFCs with different constrains limiting direct and mediated electron transfer to the anode...
October 18, 2017: BMC Microbiology
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