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Uttariya Roy, Shubhalakshmi Sengupta, Priya Banerjee, Papita Das, Avijit Bhowal, Siddhartha Datta
This study focuses on the investigation of removal of textile dye (Reactive Yellow) by a combined approach of sorption integrated with biodegradation using low cost adsorbent fly ash immobilized with Pseudomonas sp. To ensure immobilization of bacterial species on treated fly ash, fly ash with immobilized bacterial cells was characterized using Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and fluorescence microscopy. Comparative batch studies were carried out using Pseudomonas sp, fly ash and immobilized Pseudomonas sp on flyash and were observed that immobilized Pseudomonas sp on flyash acted as better decolourizing agent...
June 18, 2018: Journal of Environmental Management
Evelina L Zdorovenko, Alexander S Shashkov, Alexandra A Kadykova, Elena P Kiseleva, Victoria V Savich, Galina I Novik, Yuriy A Knirel
Strain of Pseudomonas psychrotolerans was cultured on the nutrient agar and in a liquid nutrient broth. Bacterial cells were phage-typed with bacteriophages specific to Pseudomonas. O-antigen was isolated from cells using phenol-water method and mild acid degradation. The following structures of the polysaccharides extracted were established by sugar analysis and 1D, 2D NMR spectroscopy: PSI→3)-α-D-Manp-(1→2)-α-D-Manp-(1→; PSII→3)-α-D-Rhap-(1→2)-β-D-Rhap-(1→3)-α-D-Rhap-(1→; α-D-Glcp-(1˩; 2...
June 18, 2018: Carbohydrate Research
Francine Tavares da Silva, Kamila Furtado da Cunha, Laura Martins Fonseca, Mariana Dias Antunes, Shanise Lisie Mello El Halal, Ângela Maria Fiorentin, Elessandra da Rosa Zavareze, Alvaro Renato Guerra Dias
The ultrafine fibers were produced using a polymeric blend of soy protein isolate (SPI), polyethylene oxide (PEO), and zein at a ratio of 1:1:1 (v/v/v) by electrospinning. The ginger essential oil (GEO) was encapsulated in the ultrafine fibers and the morphology, Fourier-Transform Infrared Spectroscopy (FTIR) analysis, thermal properties and relative crystallinity were evaluated. The antimicrobial activity of ginger essential oil was evaluated against five bacteria (Listeria monocytogenes, Staphylococcus aureus, Escherichia coli 0157:H7, Salmonella typhimurium, and Pseudomonas aeruginosa)...
June 18, 2018: International Journal of Biological Macromolecules
Rajani Thanissery, Daina Zeng, Raul G Doyle, Casey M Theriot
Antibiotics are considered to be the first line of treatment for mild to moderately severe Clostridium difficile infection (CDI) in humans. However, antibiotics are also risk factors for CDI as they decrease colonization resistance against C. difficile by altering the gut microbiota and metabolome. Finding compounds that selectively inhibit different stages of the C. difficile life cycle, while sparing the indigenous gut microbiota is important for the development of alternatives to standard antibiotic treatment...
2018: Frontiers in Microbiology
Leopoldo Spadea, Emanuele Tonti, Andrea Spaterna, Andrea Marchegiani
Conjunctivitis, keratoconjunctivitis, and corneal ulcers are common eye disorders frequently diagnosed in both humans and animals, and are currently treated by topical administration of eye drops containing anti-inflammatory and antibacterial agents. The current molecules often lack efficacy because infections in hypoxic tissue contain methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa ; thus, new products for the treatment of ocular pain and inflammation are needed. The use of ozone, a molecule stabilized for topical use as an ozonide, could be providential due to its anti-inflammatory and bactericidal activity in certain anterior segment pathologies, in addition to promoting tissue repair properties...
May 2018: Case Reports in Ophthalmology
Daniel Pletzer, Sarah C Mansour, Robert E W Hancock
With the antibiotic development pipeline running dry, many fear that we might soon run out of treatment options. High-density infections are particularly difficult to treat due to their adaptive multidrug-resistance and currently there are no therapies that adequately address this important issue. Here, a large-scale in vivo study was performed to enhance the activity of antibiotics to treat high-density infections caused by multidrug-resistant Gram-positive and Gram-negative bacteria. It was shown that synthetic peptides can be used in conjunction with the antibiotics ciprofloxacin, meropenem, erythromycin, gentamicin, and vancomycin to improve the treatment outcome of murine cutaneous abscesses caused by clinical hard-to-treat pathogens including all ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter cloacae) pathogens and Escherichia coli...
June 2018: PLoS Pathogens
David A Baltrus, Kelly N Orth
No abstract text is available yet for this article.
July 2018: New Phytologist
Yuli Wei, Haiyan Mao, Yunping Xu, Wencai Zou, Jiasong Fang, Jochen Blom
A novel heterotrophic, Gram-stain-negative, aerobic, rod-shaped bacterium, designated as strain MT5T , was isolated from deep seawater in the Mariana Trench and characterized phylogenetically and phenotypically. Bacterial optimal growth occurred at 28 °C (range, 4-45 °C), pH 5-7 (pH 4-11) and with 3-7 % (w/v) NaCl (0-18 %). Phylogenetic analysis based on 16S rRNA gene sequence showed that strain MT5T was related to members of the genus Pseudomonas and shared the highest sequence identities with Pseudomonas pachastrellae CCUG 46540T (99...
June 21, 2018: International Journal of Systematic and Evolutionary Microbiology
Fiorela Ghilini, Miriam Candelaria Rodriguez Gonzalez, Alejandro Guillermo Miñán, Diego Ezequiel Pissinis, Alberto Hernández Creus, Roberto Carlos Salvarezza, Patricia Laura Schilardi
The increasing incidence of infections in implantable devices has encouraged the search for biocompatible antimicrobial surfaces. In order to inhibit the bacterial adhesion and proliferation on biomaterials, several surface functionalization strategies have been developed. However, most of these strategies lead to bacteriostatic effect and only few of these are able to reach the bactericidal condition. In this work, bactericidal surfaces were designed through the functionalization of titanium surfaces with poly-L-lysine (PLL) as mediator for the incorporation of antimicrobial silver nanoparticles (AgNPs)...
June 21, 2018: ACS Applied Materials & Interfaces
A E Larue, P Swider, P Duru, D Daviaud, M Quintard, Y Davit
Optical imaging techniques for biofilm observation, like laser scanning microscopy, are not applicable when investigating biofilm formation in opaque porous media. X-ray micro-tomography (X-ray CMT) might be an alternative but it finds limitations in similarity of X-ray absorption coefficients for the biofilm and aqueous phases. To overcome this difficulty, barium sulphate was used in Davit et al. (2011) to enable high-resolution 3D imaging of biofilm via X-ray CMT. However, this approach lacks comparison with well-established imaging methods, which are known to capture the fine structures of biofilms, as well as uncertainty quantification...
June 21, 2018: Journal of Microscopy
Ju Hyoung Lim, Ho-Gun Rhie, Jeong Nam Kim
Pseudomonas fluorescens KLR101 was found to be capable of producing polyhydroxyalkanoate (PHA) using various sugars and fatty acids with carbon numbers ranging from 2 to 6. PHA granules mainly consisted of poly(3-hydroxybutyrate) homopolymer and/or poly(3-hydroxybutyrate- co -3-hydroxyvalerate) copolymer. Genomic DNA of P. fluorescens was fractionated and cloned into a lambda library, in which a 5.8-kb fragment hybridized to a heterologous phaC probe from Ralstonia eutropha was identified. In vivo expression in Klebsiella aerogenes KC2671 (pUMS), restriction mapping, Southern hybridization experiments, and sequencing data revealed that PHA biosynthesis by P...
May 11, 2018: Journal of Microbiology and Biotechnology
Yi-Xiao Ma, Xiao-Han Wu, Hui-Shi Wu, Zhan-Bo Dong, Jian-Hui Ye, Xin-Qiang Zheng, Yue-Rong Liang, Jianliang Lu
Degradation efficiency and catabolism pathways of the different methylxanthines (MXs) in isolated caffeine-tolerant strain Pseudomonas putida CT25 were comprehensively studied. The results showed that degradation efficiency of various MXs varied with number and position of the methyl-groups on the molecule ( i.e. , xanthine > 7-methylxanthine ≈ theobromine > caffeine > theophylline > 1-methylxanthine). Multiple MXs catabolism pathways coexisted in strain CT25 and a different pathway would be triggered by various MXs...
May 11, 2018: Journal of Microbiology and Biotechnology
Xiaobang Hu, Isador H Lieberman
PURPOSE: To examine the incidence of occult infection in revision spine surgeries and its correlation with preoperative inflammatory markers. METHODS: We retrospectively reviewed all patients who underwent revision spine surgery and hardware removal between 2010 and 2016. Patients who had preoperative clinical signs of infection were excluded. The hardware and surrounding tissue culture results were obtained. The patients' diagnosis and preoperative inflammatory marker (ESR, CRP, and procalcitonin) levels were recorded...
June 20, 2018: European Spine Journal
Ron D Oshri, Keren S Zrihen, Itzhak Shner, Shira Omer Bendori, Avigdor Eldar
The opportunistic pathogen Pseudomonas aeruginosa employs a hierarchical quorum-sensing network to regulate virulence factor production that cooperatively benefit the population at a cost to the individual. It has been argued that the evolution of a cooperative mutant in a quorum sensing-suppressed population would be hampered through its exploitation by neighboring non-mutant cells. It remains unclear whether mechanisms which overcome this exploitation exist. Here we investigate the regain of quorum-sensing cooperation by evolving a mutant of the lasR master quorum-sensing regulator...
June 20, 2018: ISME Journal
Valerie A Ray, Preston J Hill, C Kendall Stover, Sashwati Roy, Chandan K Sen, Li Yu, Daniel J Wozniak, Antonio DiGiandomenico
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
June 20, 2018: Scientific Reports
Jaclyn G McCutcheon, Danielle L Peters, Jonathan J Dennis
Bacteriophages DLP1 and DLP2 are capable of infecting both Stenotrophomonas maltophilia and Pseudomonas aeruginosa strains, two highly antibiotic resistant bacterial pathogens, which is unusual for phages that typically exhibit extremely limited host range. To explain their unusual cross-order infectivity and differences in host range, we have identified the type IV pilus as the primary receptor for attachment. Screening of a P. aeruginosa PA01 mutant library, a host that is susceptible to DLP1 but not DLP2, identified DLP1-resistant mutants with disruptions in pilus structural and regulatory components...
June 20, 2018: Viruses
Daneeya Ekapopphan, Athicha Srisutthakarn, Walasinee Moonarmart, Ruangrat Buddhirongawatr, Norasuthi Bangphoomi
This study aims to determine the microbiological profile and risk factors associated with antimicrobial-resistant bacteria in canine severe corneal ulcers. Thirty-two corneal and conjunctival swabs were collected from dogs with diagnosed severe corneal ulcers that presented to Prasu-Arthorn veterinary teaching hospital in Nakhon Pathom, Thailand from June 2015 to June 2016. Microorganisms were identified by means of genotypic and phenotypic approaches. Of 32 ulcers sampled, 26 (81.3%) yielded culturable microorganisms with 24 bacterial isolates and 7 fungal isolates...
June 20, 2018: Journal of Veterinary Medical Science
M Jack Borrok, Antonio DiGiandomenico, Nurten Beyaz, Gabriela M Marchetti, Arnita S Barnes, Kristen J Lekstrom, Sandrina S Phipps, Michael P McCarthy, Herren Wu, William F Dall'Acqua, Ping Tsui, Ruchi Gupta
IgG antibodies are abundantly present in the vasculature but to a much lesser extent in mucosal tissues. This contrasts with antibodies of the IgA and IgM isotype that are present at high concentration in mucosal secretions due to active delivery by the polymeric Ig receptor (pIgR). IgG is the preferred isotype for therapeutic mAb development due to its long serum half-life and robust Fc-mediated effector function, and it is utilized to treat a diverse array of diseases with antigen targets located in the vasculature, serosa, and mucosa...
June 21, 2018: JCI Insight
Suado M Abdillahi, Tobias Maaß, Gopinath Kasetty, Adam A Strömstedt, Maria Baumgarten, Ramesh Tati, Sara L Nordin, Björn Walse, Raimund Wagener, Artur Schmidtchen, Matthias Mörgelin
Collagen VI is a ubiquitous extracellular matrix component that forms extensive microfibrillar networks in most connective tissues. In this study, we describe for the first time, to our knowledge, that the collagen VI von Willebrand factor type A-like domains exhibit a broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria in human skin infections in vivo. In silico sequence and structural analysis of VWA domains revealed that they contain cationic and amphipathic peptide sequence motifs, which might explain the antimicrobial nature of collagen VI...
June 20, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Alaa Alhazmi
Spleen tyrosine kinase (SYK) is a nonreceptor tyrosine kinase which associates directly with extracellular receptors, and is critically involved in signal transduction pathways in a variety of cell types for the regulation of cellular responses. SYK is expressed ubiquitously in immune and nonimmune cells, and has a much wider biological role than previously recognized. Several studies have highlighted SYK as a key player in the pathogenesis of a multitude of diseases. Pseudomonas aeruginosa is an opportunistic gram-negative pathogen, which is responsible for systemic infections in immunocompromised individuals, accounting for a major cause of severe chronic lung infection in cystic fibrosis patients and subsequently resulting in a progressive deterioration of lung function...
June 20, 2018: Journal of Innate Immunity
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