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https://www.readbyqxmd.com/read/27917821/optimizing-cell-sourcing-for-clinical-translation-of-tissue-engineered-ears
#1
Kerry A Morrison, Benjamin P Cohen, Ope Asanbe, Xue Dong, Alice Harper, Lawrence J Bonassar, Jason A Spector
: Background . Currently, the major impediment to clinical translation of our previously described platform for the fabrication of high fidelity, patient-specific tissue engineered ears is the development of a clinically optimal cell sourcing strategy. A limited autologous auricular chondrocyte (AuC) supply in conjunction with rapid chondrocyte de-differentiation during in vitro expansion currently makes clinical translation more challenging. Mesenchymal stem cells (MSCs) offer significant promise due to their inherent chondrogenic potential, and large availability through minimally invasive procedures...
December 5, 2016: Biofabrication
https://www.readbyqxmd.com/read/27916375/the-role-of-the-adaptive-immune-system-in-burn-induced-heterotopic-ossification-and-mesenchymal-cell-osteogenic-differentiation
#2
Kavitha Ranganathan, Shailesh Agarwal, David Cholok, Shawn Loder, Jonathan Li, Hsiao Hsin Sung Hsieh, Stewart C Wang, Steven R Buchman, Benjamin Levi
BACKGROUND: Heterotopic ossification (HO) is the pathologic process of extraskeletal bone formation. Although the exact etiology remains unknown, inflammation appears to catalyze disease progression. The goal of this study is to determine the impact of the adaptive immune system on HO. METHODS: HO was induced in 8-wk-old control C57BL/6 and immunocompromised Rag1tm1Mom (Rag1 KO) male mice deficient in B- and T-lymphocytes via combined Achilles tenotomy and burn injury...
November 2016: Journal of Surgical Research
https://www.readbyqxmd.com/read/27914026/limbal-stem-cell-and-oral-mucosal-epithelial-transplantation-from-ex-vivo-cultivation-in-lscd-induced-rabbits-histology-and-immunologic-study-of-the-transplant-epithelial-sheet
#3
Napaporn Tananuvat, Kanokkan Bumroongkit, Chainarong Tocharusa, Umnat Mevatee, Aphisek Kongkaew, Somsanguan Ausayakhun
PURPOSE: To evaluate the results of cultivated limbal epithelial and oral mucosal epithelial transplantation (CLET and COMET) in limbal stem cell deficiency (LSCD)-induced rabbit model. MATERIALS AND METHODS: Six New Zealand white rabbits were divided into two groups of three rabbits each. Limbal tissue was harvested from the first group, and oral mucosal biopsy was obtained from the second group. The tissues were cultured using an explant technique with amniotic membrane as a substrate and co-culture with the 3T3 fibroblast and air-lifting method...
December 2, 2016: International Ophthalmology
https://www.readbyqxmd.com/read/27913779/comparison-of-proliferative-and-immunomodulatory-potential-of-adipose-derived-mesenchymal-stem-cells-from-young-and-geriatric-cats
#4
Lara B Zajic, Tracy L Webb, Polly Webb, Jonathan W Coy, Steve W Dow, Jessica M Quimby
OBJECTIVES: The objective of this study was to compare the ability of adipose-derived mesenchymal stem cells (aMSC) generated from young vs geriatric cats to proliferate in culture, suppress lymphocyte proliferation and undergo senescence. METHODS: Adipose tissues from five young (<5 years) and six geriatric (>10 years) cats were harvested and cryopreserved for subsequent aMSC isolation and culture. aMSC proliferation in culture was compared via determination of time until passage two and by 3-(4,5-demethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay...
December 2, 2016: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/27913088/effect-of-suction-pressures-on-cell-yield-and-functionality-of-the-adipose-derived-stromal-vascular-fraction
#5
Yuan-Wen Chen, Jing-Ru Wang, Xuan Liao, Sheng-Hong Li, Li-Ling Xiao, Biao Cheng, Guang-Hui Xie, Jian-Xing Song, Hong-Wei Liu
OBJECTIVE: The present study was performed to explore the effect of suction pressures on the cell yield of adipose-derived stromal vascular fraction (SVF) and the functionality of adipose-derived stem cells (ADSCs), situated in the SVF, and to develop optimal parameters of harvesting SVF for clinical use. METHODS: Adipose tissue was harvested from the lower abdomen of 10 patients by suction-assisted lipoplasty. Suction pressure was either -30 ± 5 kPa or -55 ± 5 kPa...
November 11, 2016: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/27906587/differentiation-of-mouse-ovarian-stem-cells-toward-oocyte-like-structure-by-coculture-with-granulosa-cells
#6
Soraya Parvari, Hossein Yazdekhasti, Zahra Rajabi, Valliollah Gerayeli Malek, Tayebeh Rastegar, Mehdi Abbasi
An increasing body of evidence has confirmed existence and function of ovarian stem cells (OSCs). In this study, a novel approach on differentiation of OSCs into oocyte-like cells (OLCs) has been addressed. Recently, different methods have been recruited to isolate and describe aspects of OSCs, but newer and more convenient strategies in isolation are still growing. Herein, a morphology-based method was used to isolate OSCs. Cell suspension of mouse neonatal ovaries was cultured and formed colonies were harvested mechanically and cultivated on mouse embryonic fibroblasts...
November 2016: Cellular Reprogramming
https://www.readbyqxmd.com/read/27903590/anterior-cruciate-ligament-derived-stem-cells-transduced-with-bmp2-accelerate-graft-bone-integration-after-acl-reconstruction
#7
Yohei Kawakami, Koji Takayama, Tomoyuki Matsumoto, Ying Tang, Bing Wang, Yutaka Mifune, James H Cummins, Ryan J Warth, Ryosuke Kuroda, Masahiro Kurosaka, Freddie H Fu, Johnny Huard
BACKGROUND: Strong graft-bone integration is a prerequisite for successful graft remodeling after reconstruction of the anterior cruciate ligament (ACL) using soft tissue grafts. Novel strategies to accelerate soft tissue graft-bone integration are needed to reduce the need for bone-tendon-bone graft harvest, reduce patient convalescence, facilitate rehabilitation, and reduce total recovery time after ACL reconstruction. HYPOTHESIS: The application of ACL-derived stem cells with enhanced expression of bone morphogenetic protein 2 (BMP2) onto soft tissue grafts in the form of cell sheets will both accelerate and improve the quality of graft-bone integration after ACL reconstruction in a rat model...
November 30, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27893630/a-comprehensive-in-vitro-comparison-of-preparation-techniques-for-fat-grafting
#8
Libor Streit, Josef Jaros, Veronika Sedlakova, Miroslava Sedlackova, Lubos Drazan, Michal Svoboda, Jakub Pospisil, Tomas Vyska, Jiri Vesely, Ales Hampl
BACKGROUND: Lipomodelling is a technique that uses patient's own fat for tissue regeneration and augmentation. The extent of regenerative effect is reported to be determined by the numbers of adipose tissue-derived stem cells (ASCs) and the viability of cells in processed adipose tissue, which, together with other factors, influence the degree of graft retention. This study addresses whether differences exist in properties of fat graft obtained by three commonly used techniques. METHODS: Adipose tissue harvested from the hypo-gastric regions of 14 patients was processed by decantation, centrifugation, and membrane-based tissue filtration...
November 21, 2016: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/27888644/myrtol-standardized-affects-mucociliary-clearance
#9
Ying Ying Li, Jing Liu, Chun Wei Li, Somasundaram Subramaniam, Siew Shuen Chao, Feng Gang Yu, Noam A Cohen, Shi Li, Yun Wang
BACKGROUND: Myrtol standardized (Gelomyrtol forte) has been shown to be effective in controlling nasal symptoms of rhinosinusitis by promoting mucociliary clearance. Our aim was to evaluate the short- and long-term effects of myrtol on ciliated columnar cells and goblet cells in an in-vitro setting. METHODS: Nasal epithelial cells were harvested (42 days) from an air-liquid interface (ALI) culture of human nasal epithelial stem/progenitor cells (hNESPCs), which was derived from biopsies of nasal inferior turbinate mucosa...
November 26, 2016: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/27886006/semi-automated-extraction-and-characterization-of-stromal-vascular-fraction-using-a-new-medical-device
#10
Alexander Hanke, Lukas Prantl, Carina Wenzel, Michael Nerlich, Gero Brockhoff, Markus Loibl, Sebastian Gehmert
INTRODUCTION: The stem cell rich Stromal Vascular Fraction (SVF) can be harvested by processing lipo-aspirate or fat tissue with an enzymatic digestion followed by centrifugation. To date neither a standardised extraction method for SVF nor a generally admitted protocol for cell application in patients exists. A novel commercially available semi-automated device for the extraction of SVF promises sterility, consistent results and usability in the clinical routine. The aim of this work was to compare the quantity and quality of the SVF between the new system and an established manual laboratory method...
November 23, 2016: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/27879591/comparison-of-endothelial-differentiation-capacities-of-human-and-rat-adipose-derived-stem-cells
#11
Hakan Orbay, Kamaljit Devi, Priscilla A Williams, Tima Dehghani, Eduardo A Silva, David E Sahar
BACKGROUND: The authors compared the endothelial differentiation capacities of human and rat adipose-derived stem cells to determine whether human adipose-derived stem cells can be a source of endothelial cells clinically. METHODS: Human and rat adipose-derived stem cells were harvested and characterized with flow cytometry and trilineage differentiation. Cells from passages III through V were fed with endothelial cell differentiation medium for up to 3 weeks. Cells were harvested after 1, 2, and 3 weeks, and endothelial differentiation was evaluated with quantitative reverse-transcriptase polymerase chain reaction, flow cytometry, and angiogenic sprouting assays...
December 2016: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/27873515/angiopoietin-1-modified-human-umbilical-cord-mesenchymal-stem-cell-therapy-for-endotoxin-induced-acute-lung-injury-in-rats
#12
Zhi Wei Huang, Ning Liu, Dong Li, Hai Yan Zhang, Ying Wang, Yi Liu, Le Ling Zhang, Xiu Li Ju
PURPOSE: Angiopoietin-1 (Ang1) is a critical factor for vascular stabilization and endothelial survival via inhibition of endothelial permeability and leukocyte- endothelium interactions. Hence, we hypothesized that treatment with umbilical cord mesenchymal stem cells (UCMSCs) carrying the Ang1 gene (UCMSCs-Ang1) might be a potential approach for acute lung injury (ALI) induced by lipopolysaccharide (LPS). MATERIALS AND METHODS: UCMSCs with or without transfection with the human Ang1 gene were delivered intravenously into rats one hour after intra-abdominal instillation of LPS to induce ALI...
January 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/27873362/influence-of-mesenchymal-stem-cells-conditioned-media-on-proliferation-of-urinary-tract-cancer-cell-lines-and-their-sensitivity-to-ciprofloxacin
#13
Malgorzata Maj, Anna Bajek, Ewelina Nalejska, Dorota Porowinska, Tomasz Kloskowski, Lidia Gackowska, Tomasz Drewa
Mesenchymal stem cells (MSCs) are known to interact with cancer cells through direct cell-to-cell contact and secretion of paracrine factors, although their exact influence on tumor progression in vivo remains unclear. To better understand how fetal and adult stem cells affect tumors, we analyzed viability of human renal (786-0) and bladder (T24) carcinoma cell lines cultured in conditioned media harvested from amniotic fluid-derived stem cells (AFSCs) and adipose-derived stem cells (ASCs). Both media reduced metabolic activity of 786-0 cells, however, decreased viability of T24 cells was noted only after incubation with conditioned medium from ASCs...
November 22, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27866962/distribution-of-muscarinic-acetylcholine-receptor-subtypes-in-the-murine-small-intestine
#14
Eleanor D Muise, Neeru Gandotra, John J Tackett, Michaela C Bamdad, Robert A Cowles
AIMS: Serotonin stimulates enterocyte turnover in the small intestine and studies suggest this is mediated by neuronal signaling via a cholinergic pathway. Distribution of the five known muscarinic receptor subtypes (mAChRs) in the small intestine has not been fully studied, and their role in intestinal growth is unknown. We hypothesized that mAChRs have distinct anatomic distributions within the bowel, and that mAChRs present within intestinal crypts mediate the effects of acetylcholine on the small intestinal mucosa...
November 17, 2016: Life Sciences
https://www.readbyqxmd.com/read/27863757/is-it-feasible-to-use-granulocyte-colony-stimulating-factor-alone-to-mobilize-progenitor-cells-in-multiple-myeloma-patients-induced-with-a-cyclophosphamide-thalidomide-and-dexamethasone-regimen
#15
Edvan de Queiroz Crusoe, Fabiana Higashi, Gracia Aparecida Martinez, José Carlos Barros, Marcelo Bellesso, Marina Rossato, Ana Cinira F Marret, Carlos Sérgio Chiattone, Vania Tietsch de Moraes Hungria
BACKGROUND: Cyclophosphamide plus thalidomide as induction for multiple myeloma patients eligible for autologous stem cell transplantation may be a limiting factor for cell mobilization. The minimum acceptable mobilized peripheral blood stem cell count to prevent deleterious effects during transplantation is 2.0×10(6) CD34(+) cells/kg. Combining other treatments to granulocyte-colony stimulating factor, such as cyclophosphamide, could overcome the mobilization limitation. The objective of this study was to assess the number of CD34(+) cells mobilized using granulocyte-colony stimulating factor with and without cyclophosphamide after induction with cyclophosphamide, thalidomide and dexamethasone...
October 2016: Revista Brasileira de Hematologia e Hemoterapia
https://www.readbyqxmd.com/read/27859277/a-cell-wall-reference-profile-for-miscanthus-bioenergy-crops-highlights-compositional-and-structural-variations-associated-with%C3%A2-development-and-organ-origin
#16
Ricardo M F da Costa, Sivakumar Pattathil, Utku Avci, Scott J Lee, Samuel P Hazen, Ana Winters, Michael G Hahn, Maurice Bosch
Miscanthus spp. are promising lignocellulosic energy crops, but cell wall recalcitrance to deconstruction still hinders their widespread use as bioenergy and biomaterial feedstocks. Identification of cell wall characteristics desirable for biorefining applications is crucial for lignocellulosic biomass improvement. However, the task of scoring biomass quality is often complicated by the lack of a reference for a given feedstock. A multidimensional cell wall analysis was performed to generate a reference profile for leaf and stem biomass from several miscanthus genotypes harvested at three developmentally distinct time points...
November 15, 2016: New Phytologist
https://www.readbyqxmd.com/read/27855196/age-related-changes-in-the-regenerative-potential-of-adipose-derived-stem-cells-isolated-from-the-prominent-fat-pads-in-human-lower-eyelids
#17
Xinhai Ye, Caihe Liao, Guangpeng Liu, Yipin Xu, Jian Tan, Zhenshun Song
The existence of multipotent adipose-derived stem cells isolated from human orbital fat (OF) tissue has shown great therapeutic potential in tissue engineering and regenerative medicine. But the use of stem cells for therapeutic applications is influenced by their proliferative and differentiation potentials, which may be affected by the age of the donor. So far there is little knowledge about the effects of donor age on the biological properties of human orbital adipose-derived stem cells (OASCs). The intraorbital fat protrusion in the lower eyelids occurs as an aging process, and the protruded fat is routinely removed during aesthetic surgeries...
2016: PloS One
https://www.readbyqxmd.com/read/27853939/wharton-s-jelly-mesenchymal-stromal-cells-as-a-feeder-layer-for-the-ex-vivo-expansion-of-hematopoietic-stem-and-progenitor-cells-a-review
#18
REVIEW
Melania Lo Iacono, Rita Anzalone, Giampiero La Rocca, Elena Baiamonte, Aurelio Maggio, Santina Acuto
In recent years, umbilical cord blood (UCB) has been widely used as an alternative source to bone marrow (BM) for transplantation of hematopoietic stem and progenitor cells (HSPCs) in a variety of hematological and non-hematological disorders. Nevertheless, the insufficient number of UCB-HSPCs for graft represents a major challenge. HSPCs ex vivo expansion prior to transplantation is a valid strategy to overcome this limit. Several attempts to optimize the expansion conditions have been reported, including the use of mesenchymal stromal cells (MSCs) as feeder layer...
November 16, 2016: Stem Cell Reviews
https://www.readbyqxmd.com/read/27837560/cryopreserved-adipose-tissue-derived-stromal-stem-cells-potential-for-applications-in-clinic-and-therapy
#19
Fabiana Zanata, Shahensha Shaik, Ram V Devireddy, Xiying Wu, Lydia Masako Ferreira, Jeffrey M Gimble
Adipose-Derived Stromal/Stem Cells (ASC) have considerable potential for regenerative medicine due to their abilities to proliferate, differentiate into multiple cell lineages, high cell yield, relative ease of acquisition, and almost no ethical concerns since they are derived from adult tissue. Storage of ASC by cryopreservation has been well described that maintains high cell yield and viability, stable immunophenotype, and robust differentiation potential post-thaw. This ability is crucial for banking research and for clinical therapeutic purposes that avoid the morbidity related to repetitive liposuction tissue harvests...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27832825/heterogeneity-of-proangiogenic-features-in-mesenchymal-stem-cells-derived-from-bone-marrow-adipose-tissue-umbilical-cord-and-placenta
#20
Wen Jing Du, Ying Chi, Zhou Xin Yang, Zong Jin Li, Jun Jie Cui, Bao Quan Song, Xue Li, Shao Guang Yang, Zhi Bo Han, Zhong Chao Han
BACKGROUND: Mesenchymal stem cells (MSCs) have been widely proven effective for therapeutic angiogenesis in ischemia animal models as well as clinical vascular diseases. Because of the invasive method, limited resources, and aging problems of adult tissue-derived MSCs, more perinatal tissue-derived MSCs have been isolated and studied as promising substitutable MSCs for cell transplantation. However, fewer studies have comparatively studied the angiogenic efficacy of MSCs derived from different tissues sources...
November 10, 2016: Stem Cell Research & Therapy
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