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Stem cell harvesting

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https://www.readbyqxmd.com/read/28916148/chemokine-receptor-directed-imaging-and-therapy
#1
REVIEW
Andreas K Buck, Antje Stolzenburg, Heribert Hänscheid, Andreas Schirbel, Katharina Lückerath, Margret Schottelius, Hans-Jürgen Wester, Constantin Lapa
The C - X - C chemokine receptor 4 (CXCR4) and its natural ligand CXCL12 are key factors in the process of cell migration, homing of hematopoietic stem cells to the bone marrow, and represent important mediators of angiogenesis and cell proliferation. The CXCR4/CXCL12 interplay can be disrupted by CXCR4 antagonists such as Plerixafor which are already in daily clinical use, i.e. for mobilization and subsequent harvesting of hematopoietic progenitor cells and stem cell transplantation. In a pathological condition, involvement in the process of metastasis and homing of cancer cells to a protective niche has been described, making CXCR4 an attractive target for imaging and treatment of malignant diseases...
September 12, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28914400/an-in-vivo-study-on-endothelialized-vascular-grafts-produced-by-autologous-biotubes-and-adipose-stem-cells-adscs
#2
Yu Chieh Tseng, Jun Neng Roan, Ying Chiang Ho, Chih Chan Lin, Ming Long Yeh
Currently, commercial synthetic vascular grafts made from Dacron and ePTFE for small-diameter, vascular applications (<6 mm) show limited reendothelization and are less compliant, often resulting in thrombosis and intimal hyperplasia. Although good blood compatibility can be achieved in autologous arteries and veins, the number of high quality harvest sites is limited, and the grafts are size-mismatched for use in the fistula or cardiovascular bypass surgery; thus, alternative small graft substitutes must be developed...
September 15, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28905449/lull-pgm-system-a-suitable-technique-to-improve-the-regenerative-potential-of-autologous-fat-grafting
#3
Paolo G Morselli, Federico A Giorgini, Claudia Pazzini, Claudio Muscari
Autologous fat grafting and methods of purification of harvested tissue have become one of the most current themes in regenerative medicine. The aim of this study was to evaluate the in vitro regenerative potential of abdomen lipoaspirates subjected to a combined washing-decantation purifying procedure, the Lull pgm System (Lull). Blood cells and stromal-vascular fraction (SVF) cells contained in the aspirates were investigated and compared with those obtained through more conventional fat-processing methods, i...
September 14, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28904691/il-21-augments-rapamycin-in-expansion-of-alpha-fetoprotein-antigen-specific-stem-cell-like-memory-t-cells-in-vitro
#4
Victor Tunje Jeza, Xiaoyi Li, Jun Chen, Zhihui Liang, Adem Onago Aggrey, Xiongwen Wu
INTRODUCTION: Alloreactive tumor specific T cells are important arsenals of the adaptive immune system in the fight against tumors. However, stem cell-like memory T cells (Tscm) provide the key to effective elimination of tumor cells. Methods for generating these T cell subsets already exist. However, they could be made more efficient. Further, they are expensive and unattainable to the resource poor laboratories. In this regard, we are hereby describing a novel in vitro allogeneic co-culture method for raising allo-restricted tumor specific Tscm cells that we developed...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28901186/recipient-adipose-derived-stem-cells-enhance-recipient-cell-engraftment-and-prolong-allotransplant-survival-in-a-miniature-swine-hind-limb-model
#5
Yur-Ren Kuo, Chien-Chang Chen, Yen-Chou Chen, Ching-Ming Chien
Donor mesenchymal stem cells (MSCs) could prolong vascularized composite allotransplantation (VCA) survival in our previous studies. However, recipient adipose tissue is easier to harvest than donor tissue for preconditioning modulation. Hence, this study investigated the efficacy of recipient autologous adipose-derived stem cells (rADSCs) for VCA survival. The heterotopic hind-limb transplantation from female donor to male recipient was performed in outbred miniature swine. Group I ( n = 6) was untreated controls...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28895851/gene-therapy-approaches-to-hemoglobinopathies
#6
REVIEW
Giuliana Ferrari, Marina Cavazzana, Fulvio Mavilio
Gene therapy for hemoglobinopathies is currently based on transplantation of autologous hematopoietic stem cells genetically modified with a lentiviral vector expressing a globin gene under the control of globin transcriptional regulatory elements. Preclinical and early clinical studies showed the safety and potential efficacy of this therapeutic approach as well as the hurdles still limiting its general application. In addition, for both beta-thalassemia and sickle cell disease, an altered bone marrow microenvironment reduces the efficiency of stem cell harvesting as well as engraftment...
October 2017: Hematology/oncology Clinics of North America
https://www.readbyqxmd.com/read/28891421/bone-marrow-aspiration-for-regenerative-orthopedic-intervention-technique-with-ultrasound-guidance-for-needle-placement
#7
Shane A Shapiro, Jennifer R Arthurs
AIM: We review relevant anatomy of the iliac crest, and describe an interventional technique to maximize harvesting of desired progenitor cells with ultrasound to guide safe trochar placement. MATERIALS & METHODS: We validated the technique on both sides of the pelvis in four human cadavers. RESULTS: Using ultrasound guidance, 32 BMA needles were placed in a safe zone along various portions of the iliac crest. CONCLUSION: Ultrasound guidance can improve accuracy of bone marrow aspirations form the iliac crest...
September 11, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28890905/single-versus-multiple-site-harvesting-techniques-for-bone-marrow-concentrate-evaluation-of-aspirate-quality-and-pain
#8
Kristin Oliver, Tariq Awan, Matthew Bayes
BACKGROUND: Bone marrow concentrate (BMC) is growing in popularity as an alternative treatment option in orthopaedics. The regenerative capacity of BMC has been linked to the number of mesenchymal stem cells (MSCs) present in the graft at the time of its clinical application. MSC counts in bone marrow aspirate (BMA) are affected by harvest technique, but controversy exists over which aspiration method optimizes cellular yield while taking patient comfort and risk into consideration. PURPOSE: To compare a single- versus multiple-site bone marrow aspiration technique to determine which would generate a sufficient volume of high-quality BMA for concentration into a BMC graft...
August 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28888019/in-vivo-characterization-of-a-3d-hybrid-scaffold-based-on-pcl-decellularized-aorta-for-tracheal-tissue-engineering
#9
Fariba Ghorbani, Lida Moradi, Mohammad Behgam Shadmehr, Shahin Bonakdar, Atosa Droodinia, Farzaneh Safshekan
INTRODUCTION: As common treatments for long tracheal stenosis are associated with several limitations, tracheal tissue engineering is considered as an alternative treatment. AIM OF STUDY: This study aimed at preparing a hybrid scaffold, based on biologic and synthetic materials for tracheal tissue engineering. MATERIALS AND METHODS: Three electrospun polycaprolactone (PCL) scaffolds, namely E1 (pure PCL), E2 (collagen-coated PCL) and E3 (PCL blended with collagen) were prepared...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28885289/mesenchymal-stem-cell-levels-of-human-spinal-tissues
#10
Liam Harris, C Thomas Vangsness
STUDY DESIGN: .: Systematic Review. OBJECTIVE: .: The aim of this study was to investigate, quantify, compare and compile the various mesenchymal stem cell tissue sources within human spinal tissues to act as a compendium for clinical and research application. SUMMARY OF BACKGROUND DATA: .: Recent years have seen a dramatic increase in academic and clinical understanding of human mesenchymal stem cells (MSCs). Previously limited to cells isolated from bone marrow, the past decade has illicited the characterization and isolation of human MSCs from adipose, bone marrow, synovium, muscle, periosteum, peripheral blood, umbilical cord, placenta and numerous other tissues...
September 6, 2017: Spine
https://www.readbyqxmd.com/read/28879669/optimising-combination-of-vascular-endothelial-growth-factor-and-mesenchymal-stem-cells-on-ectopic-bone-formation-in-scid-mice
#11
Chris H Dreyer, Kristian Kjaergaard, Nicholas Ditzel, Niklas R Jørgensen, Søren Overgaard, Ming Ding
INTRODUCTION: Insufficient blood supply may limit bone regeneration in bone defects. Vascular endothelial growth factor (VEGF) promotes angiogenesis by increasing endothelial migration. This outcome, however, could depend on time of application. Sheep mesenchymal stem cells (MSCs) in severe combined immunodeficient (SCID) mice were used in this study to evaluate optimal time points for VEGF stimulation to increase bone formation. METHODS: Twenty-eight SCID (NOD...
September 6, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28878831/the-mirage-of-long-term-vital-benefice-risk-for-the-beginning-of-life
#12
Ramona Mohora, Decebal Hudita, Silvia-Maria Stoicescu
INTRODUCTION: Cord blood is the "life" of the fetus until birth. After delivery, the newborn is "single" and forced to adapt to live using the latest resources provided by the mother. Those who believe that a newborn is just a miniature independent adult are just trusting one of the illusions of secular medicine. Cord blood contains precious cells, stem, red and white blood cells. T cells as a part of white blood cells prevent infections and other illnesses. Cochrane Database 2013 published a study reporting the role of delayed umbilical cord clamping for the benefit of infants...
January 2017: Mædica
https://www.readbyqxmd.com/read/28875007/the-impact-of-confluence-on-bone-marrow-mesenchymal-stem-bmmsc-proliferation-and-osteogenic-differentiation
#13
Faten A M Abo-Aziza, Zaki A A
Background: In the field of cellular therapy, the impact of confluence degreeonharvesting or differentiation of BMMSCs and the effect of cell-to-cell contact remain controversial. Therefore, the effect of confluence on properties of BMMSCs was studied and efficiency of confluence-associated osteogenic differentiation was identified. Materials andMethods: The impact of 20, 50, 70, 80 and 100% confluences on proliferation properties of BMMSCs, expression of ERK and p-ERK proteins and glucose consumption rate was studied...
April 1, 2017: International Journal of Hematology-oncology and Stem Cell Research
https://www.readbyqxmd.com/read/28872512/the-effects-of-adipose-derived-stem-cells-differentiated-into-endothelial-cells-and-osteoblasts-on-healing-of-critical-size-calvarial-defects
#14
Hakan Orbay, Brittany Busse, Jonathan Kent Leach, David E Sahar
Delayed vascularization and resultant resorption limits the clinical use of tissue engineered bony constructs. The objective of this study is to develop a strategy to accelerate the neovascularization of tissue-engineered bony constructs using endothelial differentiated adipose-derived stem cells (ASC). The authors harvested ASC from inguinal fat pads of male Lewis rats (n = 5) and induced toward endothelial and osteoblastic lineages. The authors created critical size calvarial defects on male Lewis rats (n = 30) and randomized the animals into 4 groups...
September 1, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28869071/role-of-transplanted-bone-marrow-cells-in-development-of-rotator-cuff-muscle-fatty-degeneration-in-mice
#15
Lawrence V Klomps, Naseem Zomorodi, H Mike Kim
BACKGROUND: Rotator cuff muscle fatty degeneration after a chronic tendon tear is an irreversible pathologic change associated with poor clinical outcomes of tendon repair, and its exact pathogenesis remains unknown. We sought to investigate the role of transplanted bone marrow cells in the development of fatty degeneration, specifically in adipocyte accumulation, using a mouse model. METHODS: Fourteen mice were divided into 2 bone marrow chimeric animal groups: bone marrow transplantation (BMT) group and reverse BMT group...
August 28, 2017: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/28865590/matrix-associated-stem-cell-transplantation-mast-in-chondral-defects-of-the-1st-metatarsophalangeal-joint-is-safe-and-effective-2-year-follow-up-in-20-patients
#16
Martinus Richter, Stefan Zech, Stefan Andreas Meissner
The aim of the study was to assess the 2-year-follow-up of matrix-associated stem cell transplantation (MAST) in chondral defects of the 1st metatarsophalangeal joint (MTPJ). In a prospective consecutive non-controlled clinical follow-up study, 20 patients with 25 chondral defect at the 1st MTPJ that were treated with MAST from October 1st, 2011 to March, 30th, 2013 were analysed. The size and location of the chondral defects range of motion (ROM), and the Visual-Analogue-Scale Foot and Ankle (VAS FA) before treatment and at follow-up were registered...
September 2017: Foot and Ankle Surgery: Official Journal of the European Society of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/28865177/surface-topography-of-silicon-nitride-affects-antimicrobial-and-osseosintegrative-properties-of-tibial-implants-in-a-murine-model
#17
Masahiro Ishikawa, Karen L de Mesy Bentley, Bryan J McEntire, B Sonny Bal, Edward M Schwarz, Chao Xie
While silicon nitride (Si3 N4 ) is an antimicrobial and osseosintegrative orthopaedic biomaterial, the contribution of surface topography to these properties is unknown. Using a methicillin-resistant strain of Staphylococcus aureus (MSRA), this study evaluated Si3 N4 implants in vitro via scanning electron microscopy (SEM) and colony forming unit (CFU) assays, and later in an established in vivo murine tibia model of implant-associated osteomyelitis. In vitro, the "as-fired" Si3 N4 implants displayed significant reductions in adherent bacteria versus machined Si3 N4 (2...
September 2, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28864616/cxcr4-ligands-the-next-big-hit
#18
Annemiek M E Walenkamp, Constantin Lapa, Ken Herrmann, Hans-Jürgen Wester
The G protein-coupled protein receptor C-X-C chemokine receptor 4 (CXCR4) is an attractive target for cancer diagnosis and treatment, as it is overexpressed in many solid and hematologic cancers. Binding of its ligand, C-X-C chemokine ligand 12 (CXCL12), results in receptor internalization and activation of several signal transduction pathways, such as phosphoinositide 3-kinase/protein kinase B, which are critical in cell proliferation, angiogenesis, development of metastasis, and survival. Also, the CXCR4-CXCL12 axis is involved in the interaction between hematopoietic stem cells (as well as hematologic and solid tumor cells) and their protective microenvironment...
September 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28861788/cellular-analysis-of-adult-neural-stem-cells-for-investigating-prion-biology
#19
Cathryn L Haigh
Traditional primary and secondary cell cultures have been used for the investigation of prion biology and disease for many years. While both types of cultures produce highly valid and immensely valuable results, they also have their limitations; traditional cell lines are often derived from cancers, therefore subject to numerous DNA changes, and primary cultures are labor-intensive and expensive to produce requiring sacrifice of many animals. Neural stem cell (NSC) cultures are a relatively new technology to be used for the study of prion biology and disease...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28860595/intestinal-stem-cells-contribute-to-the-maturation-of-the-neonatal-small-intestine-and-colon-independently-of-digestive-activity
#20
Hirotsugu Yanai, Naho Atsumi, Toshihiro Tanaka, Naohiro Nakamura, Yoshihiro Komai, Taichi Omachi, Kiyomichi Tanaka, Kazuhiko Ishigaki, Kazuho Saiga, Haruyuki Ohsugi, Yoko Tokuyama, Yuki Imahashi, Shuichi Ohe, Hiroko Hisha, Naoko Yoshida, Keiki Kumano, Masanori Kon, Hiroo Ueno
The murine intestine, like that of other mammalians, continues to develop after birth until weaning; however, whether this occurs in response to an intrinsic developmental program or food intake remains unclear. Here, we report a novel system for the allotransplantation of small intestine and colon harvested from Lgr5 (EGFP-IRES-CreERT2/+); Rosa26 (rbw/+) mice immediately after birth into the subrenal capsule of wild-type mice. By histological and immunohistochemical analysis, the developmental process of transplanted small intestine and colon was shown to be comparable with that of the native tissues: mature intestines equipped with all cell types were formed, indicating that these organs do not require food intake for development...
August 31, 2017: Scientific Reports
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