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Stem cell harvesting

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https://www.readbyqxmd.com/read/29781904/adipose-stromal-vascular-fraction-gel-grafting-a-new-method-for-tissue-volumization-and-rejuvenation
#1
Yao Yao, Junrong Cai, Pan Zhang, Yunjun Liao, Yi Yuan, Ziqing Dong, Feng Lu
BACKGROUND: The clinical outcomes of fat grafting vary and are technique-dependent. Stromal vascular fraction (SVF) gel is a novel, mechanically processed fat product with high concentrations of adipose tissue-derived stem cells and other SVF cells. This study evaluated the volumization and rejuvenation effects of SVF-gel. OBJECTIVE: This study evaluated the volumization and rejuvenation effects of SVF-gel. METHODS: This retrospective, single-center study included 126 patients who underwent SVF-gel grafting and 78 who underwent conventional lipoinjection for various indications from March 2015 to February 2017...
May 16, 2018: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/29781821/basic-science-and-clinical-application-of-reamed-sources-for-autogenous-bone-graft-harvest
#2
Thomas F Higgins, Lucas S Marchand
Autologous bone graft remains the only clinically available source of graft material with osteogenic, osteoinductive, and osteoconductive properties. Although iliac crest autologous bone graft has long served as the benchmark, reamed autogenous bone graft offers several advantages. Reamed autograft has a biochemical and cellular profile that is at least equivalent, and perhaps superior, to that of iliac crest autograft. In addition, larger volumes of reamed autograft can be obtained via less-invasive techniques, giving surgeons an accessible source of mesenchymal stem cells that can be reliably and repeatedly harvested...
May 16, 2018: Journal of the American Academy of Orthopaedic Surgeons
https://www.readbyqxmd.com/read/29780916/visual-acuity-of-20-32-13-5-years-after-a-retinal-pigment-epithelium-and-choroid-graft-transplantation
#3
Elsbeth J T van Zeeburg, Kristel J M Maaijwee, Jan C van Meurs
Purpose: To present the 13.5-year-survival of an autologous retinal pigment epithelium (RPE) and choroid graft transplantation with good visual acuity results. Observations: A 72-year old patient presented with a 5-weeks-old visual acuity deterioration to excentric finger counting at half a meter. Fundoscopy showed a fibrotic macular scar, a large subretinal hemorrhage, partly recent, combined with intraretinal fluid, blood, and hard exudates. RPE-choroid graft surgery was performed, and visual acuity improved to 20/32, and maintained up until 13...
June 2018: American Journal of Ophthalmology Case Reports
https://www.readbyqxmd.com/read/29777574/factors-influencing-the-umbilical-cord-blood-stem-cell-industry-an-evolving-treatment-landscape
#4
REVIEW
Carla Dessels, Marco Alessandrini, Michael Sean Pepper
Hematopoietic stem cell transplantation (HSCT) is common practice today for life threatening malignant and non-malignant diseases of the blood and immune systems. Umbilical cord blood (UCB) is rich in hematopoietic stem cells (HSCs) and is an attractive alternative to harvesting HSCs from bone marrow or when mobilized into peripheral blood. One of the most appealing attributes of UCB is that it can be banked for future use and hence provides an off-the-shelf solution for patients in urgent need of a transplantation...
May 18, 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29769672/pancreas-regeneration
#5
REVIEW
Qiao Zhou, Douglas A Melton
The pancreas is made from two distinct components: the exocrine pancreas, a reservoir of digestive enzymes, and the endocrine islets, the source of the vital metabolic hormone insulin. Human islets possess limited regenerative ability; loss of islet β-cells in diseases such as type 1 diabetes requires therapeutic intervention. The leading strategy for restoration of β-cell mass is through the generation and transplantation of new β-cells derived from human pluripotent stem cells. Other approaches include stimulating endogenous β-cell proliferation, reprogramming non-β-cells to β-like cells, and harvesting islets from genetically engineered animals...
May 2018: Nature
https://www.readbyqxmd.com/read/29769009/mesenchymal-stem-cell-research-in-veterinary-medicine
#6
Mudasir Bashir Gugjoo, Amarpal, Vikash Chandra, Mohd Yaqoob Wani, Kuldeep Dhama, Gutulla Taru Sharma
BACKGROUND: The stem cells due to their unique characteristic features like self-renewal, multiplication, immuno-modulation and differentiation, are seen and hoped to bring new horizons in the field of biology, in general and medicine, in particular. OBJECTIVE: Among various stem cell types, currently mesenchymal stem cells (MSCs) are extensively studied in regenerative medicine owing to their readily available sources, easy harvesting and ability to differentiate both into mesodermal as well as non-mesodermal tissues with little associated ethical issues...
May 16, 2018: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29766931/-in-vitro-differentiation-of-neural-cells-from-human-adipose-tissue-derived-stromal-cells
#7
Shruti D Dave, Chetan N Patel, Aruna V Vanikar, Hargovind L Trivedi
Background: Stem cells, including neural stem cells (NSCs), are endowed with self-renewal capability and hence hold great opportunity for the institution of replacement/protective therapy. We propose a method for in vitro generation of stromal cells from human adipose tissue and their differentiation into neural cells. Materials and Methods: Ten grams of donor adipose tissue was surgically resected from the abdominal wall of the human donor after the participants' informed consents...
May 2018: Neurology India
https://www.readbyqxmd.com/read/29766024/integrating-hdad5-35-vectors-as-a-new-platform-for-hsc-gene-therapy-of-hemoglobinopathies
#8
Chang Li, Nikoletta Psatha, Hongjie Wang, Manvendra Singh, Himanshu Bhusan Samal, Wenli Zhang, Anja Ehrhardt, Zsuzsanna Izsvák, Thalia Papayannopoulou, André Lieber
We generated an integrating, CD46-targeted, helper-dependent adenovirus HDAd5/35++ vector system for hematopoietic stem cell (HSC) gene therapy. The ∼12-kb transgene cassette included a β-globin locus control region (LCR)/promoter driven human γ-globin gene and an elongation factor alpha-1 (EF1α)-mgmtP140K expression cassette, which allows for drug-controlled increase of γ-globin-expressing erythrocytes. We transduced bone marrow lineage-depleted cells from human CD46-transgenic mice and transplanted them into lethally irradiated recipients...
June 15, 2018: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/29764889/autologous-stem-cells-in-achilles-tendinopathy-ascat-protocol-for-a-phase-iia-single-centre-proof-of-concept-study
#9
Andrew J Goldberg, Razi Zaidi, Deirdre Brooking, Louise Kim, Michelle Korda, Lorenzo Masci, Ruth Green, Paul O'Donnell, Roger Smith
INTRODUCTION: Achilles tendinopathy (AT) is a cause of pain and disability affecting both athletes and sedentary individuals. More than 150 000 people in the UK every year suffer from AT.While there is much preclinical work on the use of stem cells in tendon pathology, there is a scarcity of clinical data looking at the use of mesenchymal stem cells to treat tendon disease and there does not appear to be any studies of the use of autologous cultured mesenchymal stem cells (MSCs) for AT...
May 14, 2018: BMJ Open
https://www.readbyqxmd.com/read/29764513/cellular-therapy-with-human-autologous-adipose-derived-adult-cells-of-stromal-vascular-fraction-for-alopecia-areata
#10
Rami Anderi, Nehman Makdissy, Albert Azar, Francine Rizk, Aline Hamade
BACKGROUND: Most common forms of hair loss (alopecia) are caused by aberrant hair follicle cycling and changes in hair follicle morphology. However, current treatments for alopecia do not specifically target these processes. Adipose-derived stromal vascular cells (ADSVCs) that can be harvested from fat cells are one of the latest breakthroughs in the aesthetic field. The potential use of stem cell-based therapies (SCBT) for the repair and regeneration of various tissues and organs offers a paradigm shift that may provide alternative therapeutic solutions, which can be applied to prevent hair loss...
May 15, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29764508/adipose-derived-stem-cells-for-treatment-of-chronic-ulcers-current-status
#11
REVIEW
Jens Selch Holm, Navid Mohamadpour Toyserkani, Jens Ahm Sorensen
Chronic ulcers remain a difficult challenge in healthcare systems. While treatment options are limited, stem cells may be a novel alternative. Adipose-derived stem cells (ADSC) have become increasingly popular compared with bone marrow-derived stem cells as they are far easier to harvest. To summarize the current status of treating chronic ulcers with ADSC, this systematic review includes all clinical trials on the subject from PubMed and EmBase, as well as all registered clinical trials on ClinicalTrials.Gov...
May 15, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29756539/skin-regeneration-with-a-scaffold-of-predefined-shape-and-bioactive-peptide-hydrogels
#12
Heejung Im, Su Hee Kim, Soo Hyun Kim, Youngmee Jung
We developed a highly elastic customized scaffold for soft-tissue regeneration and combined them with bioactive hydrogels with stem-cell-inducing ability. This was done to mimic mechanical properties of native soft tissues and improve the viability of transplanted cells as well as efficiency of tissue regeneration. The proposed study was aimed at evaluating various characteristics of scaffolds and investigating their tissue regenerating ability. Finger shaped porous scaffolds were successfully fabricated by an indirect 3D printing of poly (L-lactide-co-ε-caprolactone) (PLCL) which provides a high elasticity for soft tissue engineering...
May 14, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29753677/multicenter-phase-1-2-application-of-adenovirus-specific-t-cells-in-high-risk-pediatric-patients-after-allogeneic-stem-cell-transplantation
#13
Winnie Ip, Juliana M F Silva, Hubert Gaspar, Arindam Mitra, Shreenal Patel, Kanchan Rao, Robert Chiesa, Persis Amrolia, Kimberly Gilmour, Gul Ahsan, Mary Slatter, Andrew R Gennery, Robert F Wynn, Paul Veys, Waseem Qasim
BACKGROUND: Adenovirus (ADV) reactivation can cause significant morbidity and mortality in children after allogeneic stem cell transplantation. Antiviral drugs can control viremia, but viral clearance requires recovery of cell-mediated immunity. METHOD: This study was an open-label phase 1/2 study to investigate the feasibility of generating donor-derived ADV-specific T cells (Cytovir ADV, Cell Medica) and to assess the safety of pre-emptive administration of ADV-specific T cells in high-risk pediatric patients after allogeneic hematopoietic stem cell transplantation (HSCT) to treat adenoviremia...
May 9, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29753580/acceleration-of-diabetic-wound-healing-with-adipose-derived-stem-cells-endothelial-differentiated-stem-cells-and-topical-conditioned-medium-therapy-in-a-swine-model
#14
Robin F Irons, Kevin W Cahill, Deviney A Rattigan, Joseph H Marcotte, Marc W Fromer, Shaohua Chang, Ping Zhang, Eric M Behling, Kathryn C Behling, Francis J Caputo
OBJECTIVE: The purpose of our study was to investigate the effect of adipose-derived stem cells (ASCs), endothelial-differentiated ASCs (EC/ASCs), and various conditioned media (CM) on wound healing in a diabetic swine model. We hypothesized that ASC-based therapies would accelerate wound healing. METHODS: Diabetes was induced in four Yorkshire swine through intravenous injection of streptozotocin. ASCs were harvested from flank fat and cultured in either M199 or EGM-2 medium...
May 9, 2018: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/29745047/systematic-review-of-the-efficacy-of-fat-grafting-and-platelet-rich-plasma-for-wound-healing
#15
Oliver J Smith, Muholan Kanapathy, Ankur Khajuria, Max Prokopenko, Nadine Hachach-Haram, Haroon Mann, Ash Mosahebi
Adipose-derived stem cells found in fat grafts may have significant healing properties. When fat is combined with autologous platelet-rich plasma (PRP), there may be enhanced healing effects due to the pro-angiogenic and anti-inflammatory effects of PRP. This study aimed to evaluate the current evidence on fat grafting in combination with PRP for wound healing to establish the efficacy of this technique. A comprehensive search in the MEDLINE, EMBASE, CENTRAL, Science Citation Index, and Google Scholar databases (to March 2017) was conducted to identify studies on fat grafting and PRP for wound healing...
May 9, 2018: International Wound Journal
https://www.readbyqxmd.com/read/29743810/auricular-cartilage-regeneration-with-adipose-derived-stem-cells-in-rabbits
#16
Se-Joon Oh, Hee-Young Park, Kyung-Un Choi, Sung-Won Choi, Sung-Dong Kim, Soo-Keun Kong, Kyu-Sup Cho
Tissue engineering cell-based therapy using induced pluripotent stem cells and adipose-derived stem cells (ASCs) may be promising tools for therapeutic applications in tissue engineering because of their abundance, relatively easy harvesting, and high proliferation potential. The purpose of this study was to investigate whether ASCs can promote the auricular cartilage regeneration in the rabbit. In order to assess their differentiation ability, ASCs were injected into the midportion of a surgically created auricular cartilage defect in the rabbit...
2018: Mediators of Inflammation
https://www.readbyqxmd.com/read/29730288/comparative-characterization-of-stem-cells-from-human-exfoliated-deciduous-teeth-dental-pulp-and-bone-marrow-derived-mesenchymal-stem-cells
#17
Ryo Kunimatsu, Kengo Nakajima, Awada Tetsuya, Yuji Tsuka, Takaharu Abe, Kazuyo Ando, Tomoka Hiraki, Aya Kimura, Kotaro Tanimoto
OBJECTIVES: Mesenchymal stem cells (MSCs) are used clinically in tissue engineering and regenerative medicine. The proliferation and osteogenic differentiation potential of MSCs vary according to factors such as tissue source and cell population heterogeneity. Dental tissue has received attention as an easily accessible source of high-quality stem cells. In this study, we compared the in vitro characteristics of dental pulp stem cells from deciduous teeth (SHED), human dental pulp stem cells (hDPSCs), and human bone marrow mesenchymal stem cells (hBMSCs)...
May 3, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29722898/lw106-a-novel-inhibitor-of-ido1-suppresses-tumor-progression-by-limiting-stroma-immune-crosstalk-and-cancer-stem-cell-enrichment-in-the-tumor-microenvironment
#18
Rong Fu, Yi-Wei Zhang, Hong-Mei Li, Wen-Cong Lv, Li Zhao, Qing-Long Guo, Tao Lu, Stephen J Weiss, Zhi-Yu Li, Zhao-Qiu Wu
BACKGROUND AND PURPOSE: Indoleamine 2,3-dioxygenase 1 (IDO1) is emerging as an important new therapeutic target for treatment of malignant tumors characterized by dysregulated tryptophan metabolism. However, the antitumor efficacy of existing small-molecule inhibitors of IDO1 is still unsatisfactory and the underlying mechanism remains largely undefined. Hence, we discovered a novel potent small-molecule inhibitor of IDO1, LW106, and studied its antitumor effects and the underlying mechanisms in two Tumor models...
May 3, 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29722765/making-them-commit-strategies-to-influence-phenotypic-differentiation-in-mesenchymal-stem-cells
#19
Alessio Giai Via, Mary B McCarthy, Laura de Girolamo, Enrico Ragni, Francesco Oliva, Nicola Maffulli
Tendon injuries, bone defects, and cartilage defects are complex clinical conditions leading to pain and dysfunctions. Tendon, bone, and cartilage are highly specialized and organized tissues, and the self-healing may be limited by their histologic features, or impaired by the local conditions. Furthermore, the resultant tissue often shows inferior properties compared with native tissue, leading to high rates of reruptures and revision surgeries. A growing field of research has explored tendon, bone, and cartilage regeneration using mesenchymal stem cells (MSCs), because of their multipotency, and because they are relatively easy to harvest...
June 2018: Sports Medicine and Arthroscopy Review
https://www.readbyqxmd.com/read/29720250/efficient-induction-of-functional-ameloblasts-from-human-keratinocyte-stem-cells
#20
Xuefeng Hu, Jyh-Wei Lee, Xi Zheng, Junhua Zhang, Xin Lin, Yingnan Song, Bingmei Wang, Xiaoxiao Hu, Hao-Hueng Chang, Yiping Chen, Chun-Pin Lin, Yanding Zhang
BACKGROUND: Although adult human tissue-derived epidermal stem cells are capable of differentiating into enamel-secreting ameloblasts and forming teeth with regenerated enamel when recombined with mouse dental mesenchyme that possesses odontogenic potential, the induction rate is relatively low. In addition, whether the regenerated enamel retains a running pattern of prism identical to and acquires mechanical properties comparable with human enamel indeed warrants further study. METHODS: Cultured human keratinocyte stem cells (hKSCs) were treated with fibroblast growth factor 8 (FGF8) and Sonic hedgehog (SHH) for 18 h or 36 h prior to being recombined with E13...
May 2, 2018: Stem Cell Research & Therapy
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