keyword
MENU ▼
Read by QxMD icon Read
search

Stem cell harvesting

keyword
https://www.readbyqxmd.com/read/28427135/-effects-of-allogeneic-bone-marrow-mesenchymal-stem-cells-on-polarization-of-peritoneal-macrophages-in-rats-with-sepsis
#1
Y H Zheng, B Xiong, Y Y Deng, W Lai, S Y Zheng, H N Bian, Z A Liu, Z F Huang, C W Sun, H H Li, H M Luo, L H Ma, H X Chen
Objective: To explore the effects of allogeneic bone marrow mesenchymal stem cells (BMSCs) on polarization of peritoneal macrophages isolated from rats with sepsis induced by endotoxin/lipopolysaccharide (LPS). Methods: (1) BMSCs were isolated, cultured and purified from 5 SD rats with whole bone marrow adherent method. The third passage of cells were collected for morphologic observation, detection of expressions of stem cell surface markers CD29, CD44, CD45, and CD90 with flow cytometer, and identification of osteogenic and adipogenic differentiation...
April 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28425078/involvement-of-interstitial-cells-of-cajal-in-bladder-dysfunction-in-mice-with-experimental-autoimmune-encephalomyelitis
#2
Zhibo Jin, Yinghui Ding, Rui Xue, Zhankui Jia, Zhenlin Huang, Yafei Ding, Chaohui Gu, Jinjian Yang
BACKGROUND: Bladder dysfunction is an important symptom of experimental autoimmune encephalomyelitis (EAE). Our previous study showed that EAE-induced upregulation of the E-prostanoid receptor 3 (EP3) and E-prostanoid receptor 4 (EP4) in the bladder was accompanied by bladder dysfunction. Although many other studies have evaluated the lower urinary tract symptoms in multiple sclerosis, the mechanism remains unclear. OBJECTIVES: To investigate the effects of interstitial cells of Cajal (ICC) on bladder dysfunction in a novel neurogenic bladder model induced by experimental autoimmune encephalomyelitis...
April 19, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28420460/-bone-marrow-mesenchymal-stem-cells-modulated-the-inflammatory-response-by-regulating-the-expression-of-il-4-and-rage-products-in-the-rats-with-mods
#3
Xia Zhou, Guanghui Xiu, Yichao Zhu, Xiaolei Chen, Wei Xiong, Xinghua Pan, Jie Sun, Bin Ling
OBJECTIVE: To investigate the underlying mechanism of bone marrow mesenchymal stem cells (BMSC) modulating the inflammatory response during the multiple organ dysfunction syndrome (MODS), especially the expression of inflammatory cytokines, which will provide new theoretical and experimental basis of MODS in clinic. METHODS: BMSC of Sprague-Dawley (SD) rat (female, 4 weeks) was extracted and cultivated, and the 4th passage were used in experimental study. According to the random number table, 60 female SD rats were divided into three groups (n = 20 per group): sham group, MODS group, BMSC group...
April 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28415516/acceleration-of-bone-regeneration-in-bioactive-glass-gelatin-composite-scaffolds-seeded-with-bone-marrow-derived-mesenchymal-stem-cells-over-expressing-bone-morphogenetic-protein-7
#4
Saeid Kargozar, Seyed Jafar Hashemian, Mansooreh Soleimani, Peiman Brouki Milan, Mohammad Askari, Vahid Khalaj, Ali Samadikuchaksaraie, Sepideh Hamzehlou, Amir Reza Katebi, Noorahmad Latifi, Masoud Mozafari, Francesco Baino
In this research, the osteoinduction effect of a novel variant of bone morphogenetic protein-7 (BMP-7), delivered through bone marrow mesenchymal stem cells (BM-MSCs) seeded on bioactive glass/gelatin nanocomposite scaffolds, was evaluated in a calvarial critical size defect in rats. After being harvested and characterized in vitro, BM-MSCs were infected by a plasmid vector containing BMP-7 encoding gene enriched with a heparin-binding site (B2BMP-7) to assess its osteogenic effects in vivo. The animals were randomly categorized into three groups receiving the scaffold alone (group I), the scaffold seeded with BM-MSCs (group II), and the scaffold seeded with manipulated BM-MSCs (group III)...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28413812/quantitative-3d-time-lapse-imaging-of-muscle-progenitors-in-skeletal-muscle-of-live-mice
#5
Micah T Webster, Tyler Harvey, Chen-Ming Fan
For non-optically clear mammalian tissues, it is now possible to use multi-photon microscopy to penetrate deep into the tissue and obtain detailed single cell images in a live animal, i.e., intravital imaging. This technique is in principle applicable to any fluorescently marked cell, and we have employed it to observe stem cells during the regenerative process. Stem cell-mediated skeletal muscle regeneration in the mouse model has been classically studied at specific time points by sacrificing the animal and harvesting the muscle tissue for downstream analyses...
December 20, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28413469/repairing-effects-of-icam-1-expressing-mesenchymal-stem-cells-in-mice-with-autoimmune-thyroiditis
#6
Shifeng Ma, Xiuhui Chen, Lihui Wang, Ying Wei, Yongqing Ni, Yanan Chu, Yuanlin Liu, Heng Zhu, Rongxiu Zheng, Yi Zhang
The aim of the present study was to determine the repairing effects of intercellular adhesion molecule (ICAM)-1-expressing mesenchymal stem cells (MSCs) in mice with autoimmune thyroiditis. Following induction of an experimental autoimmune thyroiditis (EAT) model, mice were randomly divided into the following groups (n=10 each): i) Normal control; and experimental groups that were subject to EAT induction, including ii) EAT model; and iii) primary MSC; iv) C3H10T1/2/MSC; v) C3H10T1/2-MIGR1/MSC; and vi) C3H10T1/2-MIGR1-ICAM-1/MSC, which were all administered the relevant cells...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28411882/in-vitro-generation-of-interleukin-10-secreting-b-regulatory-cells-from-donor-adipose-tissue-derived-mesenchymal-stem-cells-and-recipient-peripheral-blood-mononuclear-cells-for-potential-cell-therapy
#7
Kunal S Gupte, Aruna V Vanikar, Hargovind L Trivedi, Chetan N Patel, Jignesh V Patel
BACKGROUND: Interleukin-10 secreting B-cells are a major subset of B-regulatory cells (B-regs), commonly recognized as CD19(+)/38(hi)/24(hi)/IL10(+). They carry out immunomodulation by release of specific cytokines and/or cell-to-cell contact. We have generated B-regs in-vitro from donor adipose tissue derived mesenchymal stem cells (AD-MSC) and renal allograft recipient (RAR) peripheral blood mononuclear cells (PBMC) for potential cell therapy. MATERIAL AND METHODS: Mononuclear cells separated by density gradient centrifugation from 50 ml anti-coagulated blood of 15-RAR and respective donors were analysed for baseline B-regs using appropriate antibodies...
February 2017: Biomedical Journal
https://www.readbyqxmd.com/read/28406800/simple-limbal-epithelial-transplantation
#8
Virender S Sangwan, John A H Sharp
PURPOSE OF REVIEW: Simple limbal epithelial transplant (SLET) is a technique for addressing limbal stem cell deficiency. Limbal tissue from a donor eye, typically the patient's fellow healthy eye, is transplanted onto an amniotic membrane attached to the surface of the diseased eye. SLET was developed to address limitations of other techniques. Specifically, the technical difficulty of ex-vivo expansion of cells required in some techniques and the larger amount of valuable limbal tissue harvested in techniques not relying on ex-vivo expansion...
April 12, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28401887/bone-marrow-mesenchymal-stem-cells-combine-with-treated-dentin-matrix-to-build-biological-root
#9
Shiwei Luo, Fei Pei, Wen Zhang, Weihua Guo, Rui Li, Wei He, Weidong Tian
Treated dentin matrix (TDM) as a kind of scaffolding material has been proved odontogenic induction ability on dental-derived stem cells. Given the limited resources of dental stem cells, it is necessary to seek new seed cell which easily obtained. Jaw bone marrow mesenchymal stem cell (JBMMSC) as non-dental-derived stem cell relates to the development of teeth and jaws which suggest us JBMMSCs could act as a new seed cell for tooth tissue engineering. To assess the odontogenic and osteogenic potential of JBMMSCs, cells were induced by TDM extraction in vitro and combined with TDM in vivo...
April 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28401032/modern-trends-in-lipomodeling
#10
REVIEW
Ahmed Hassan El-Sabbagh
Lipomodeling is the process of relocating autologous fat to change the shape, volume, consistency, and profile of tissues, with the aim of reconstructing, rejuvenating, and regenerating body features. There have been several important advancements in lipomodeling procedures during the last thirty years. Four clinical steps are important for the success of engraftment: fat harvesting, fat processing, fat reinjection, and preconditioning of the recipient site. With the discovery of adipose derived stem cells and dedifferentiated cells, fat cells become a major tool of regenerative medicine...
2017: GMS Interdisciplinary Plastic and Reconstructive Surgery DGPW
https://www.readbyqxmd.com/read/28396251/gene-expression-profiling-of-bone-marrow-mesenchymal-stem-cells-from-osteogenesis-imperfecta-patients-during-osteoblast-differentiation
#11
Carla Martins Kaneto, Patrícia S Pereira Lima, Karen Lima Prata, Jane Lima Dos Santos, João Monteiro de Pina Neto, Rodrigo Alexandre Panepucci, Houtan Noushmehr, Dimas Tadeu Covas, Francisco José Alburquerque de Paula, Wilson Araújo Silva
Mesenchymal stem cells (MSCs) are precursors present in adult bone marrow that are able to differentiate into osteoblasts, adipocytes and chondroblasts that have gained great importance as a source for cell therapy. Recently, a number of studies involving the analysis of gene expression of undifferentiated MSCs and of MSCs in the differentiation into multiple lineage processes were observed but there is no information concerning the gene expression of MSCs from Osteogenesis Imperfecta (OI) patients. Osteogenesis Imperfecta is characterized as a genetic disorder in which a generalized osteopenia leads to excessive bone fragility and severe bone deformities...
April 7, 2017: European Journal of Medical Genetics
https://www.readbyqxmd.com/read/28392903/emergence-of-signs-of-neural-cells-after-exposure-of-bone-marrow-derived-mesenchymal-stem-cells-to-fetal-brain-extract
#12
Iman Razeghian Jahromi, Davood Mehrabani, Ali Mohammadi, Mohammad Mahdi Ghahramani Seno, Mehdi Dianatpour, Shahrokh Zare, Amin Tamadon
OBJECTIVES: Nowadays much effort is being invested in order to diagnose the mechanisms involved in neural differentiation. By clarifying this, making desired neural cells in vitro and applying them into diverse neurological disorders suffered from neural cell malfunctions could be a feasible choice. Thus, the present study assessed the capability of fetal brain extract (FBE) to induce rat bone marrow-derived mesenchymal stem cells (BM-MSCs) toward neural cells. MATERIALS AND METHODS: For this purpose, BM-MSCs were collected from rats and cultured and their mesenchymal properties were confirmed...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28388958/pre-incubation-with-hucmsc-exosomes-prevents-cisplatin-induced-nephrotoxicity-by-activating-autophagy
#13
Bingying Wang, Haoyuan Jia, Bin Zhang, Juanjuan Wang, Cheng Ji, Xueming Zhu, Yongmin Yan, Lei Yin, Jing Yu, Hui Qian, Wenrong Xu
BACKGROUND: The administration of cisplatin is limited due to its nephrotoxic side effects, and prevention of this nephrotoxicity of cisplatin is difficult. Mesenchymal stem cell (MSC)-derived exosomes have been implicated as a novel therapeutic approach for tissue injury. In this study, we demonstrated that the pretreatment of human umbilical cord MSC-derived exosomes (hucMSC-Ex) can prevent the development of cisplatin-induced renal toxicity by activation of autophagy in vitro and in vivo...
April 8, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28386853/assessment-of-bone-regeneration-of-a-tissue-engineered-bone-complex-using-human-dental-pulp-stem-cells-poly-%C3%AE%C2%B5-caprolactone-biphasic-calcium-phosphate-scaffold-constructs-in-rabbit-calvarial-defects
#14
Natkrita Wongsupa, Thongchai Nuntanaranont, Suttatip Kamolmattayakul, Nuttawut Thuaksuban
The objective of the present study was to investigate the effect of a fabricated combination of poly-ɛ-caprolactone (PCL)-biphasic calcium phosphate (BCP) with the modified melt stretching and multilayer deposition (mMSMD) technique on human dental pulp stem cell (hDPSC) differentiation to be osteogenic like cells for bone regeneration of calvarial defects in rabbit models. hDPSCs extracted from human third molars were seeded onto mMSMD PCL-BCP scaffolds and the osteogenic gene expression was tested prior to implantation in vivo...
May 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28386120/from-skeletal-muscle-to-stem-cells-an-innovative-and-minimally-invasive-process-for-multiple-species
#15
J Ceusters, J-Ph Lejeune, C Sandersen, A Niesten, L Lagneaux, D Serteyn
Bone marrow and adipose tissue represent the two most commonly exploited sources of adult mesenchymal stem cells for musculoskeletal applications. Unfortunately the sampling of bone marrow and fat tissue is invasive and does not always lead to a sufficient number of cells. The present study describes a novel sampling method based on microbiopsy of skeletal muscle in man, pigs, dogs and horses. The process includes explant of the sample, Percoll density gradient for isolation and subsequent culture of the cells...
April 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28386069/tissue-harvesting-site-and-culture-medium-affect-attachment-growth-and-phenotype-of-ex-vivo-expanded-oral-mucosal-epithelial-cells
#16
Rakibul Islam, Jon Roger Eidet, Reza A Badian, Marit Lippestad, Edward Messelt, May Griffith, Darlene A Dartt, Tor Paaske Utheim
Transplantation of cultured oral mucosal epithelial cells (OMECs) is a promising treatment strategy for limbal stem cell deficiency. In order to improve the culture method, we investigated the effects of four culture media and tissue harvesting sites on explant attachment, growth, and phenotype of OMECs cultured from Sprague-Dawley rats. Neither choice of media or harvesting site impacted the ability of the explants to attach to the culture well. Dulbecco's modified Eagle's medium/Ham's F12 (DMEM) and Roswell Park Memorial Institute 1640 medium (RPMI) supported the largest cellular outgrowth...
April 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28379122/recipient-adipose-derived-stem-cells-enhance-recipient-cell-engraftment-and-prolong-allotransplant-survival-in-a-miniature-swine-hind-limb-model
#17
Yur-Ren Kuo, Chien-Chang Chen, Yen-Chou Chen, Ching-Ming Chien
Donor mesenchymal stem cells could prolong vascularized composite allotransplant (VCA) survival in our previous study.However, recipient adipose tissue is easier to harvest than donor tissue for pre-conditioning modulation. Hence, this study investigated the efficacy of recipient autologous adipose-derived stem cells (rASCs) for VCA survival. The heterotopic hind-limb transplantation from female donor to male recipient was performed in out-bred miniature swine. Group-I (N=6) were untreated controls. Group-II (N=4) obtained rASC infusions (given on week 0, +1, +2, and +3)...
March 17, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28372703/editorial-commentary-thinking-inside-the-box-yields-the-possibility-of-harvesting-stem-cells-from-inside-the-injured-knee
#18
EDITORIAL
Michael J Rossi
Looking to the injured knee for potential stem cell harvesting has attractive benefits. Stem cells can be successfully harvested but the concentrations with the current technology are lower than bone marrow or lipoaspirate sites. The technique of utilizing the knee effusion and tissue by-products after cruciate ligament injury shows future promise.
April 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28370348/microvesicles-shuttled-mir-200b-attenuate-experimental-colitis-associated-intestinal-fibrosis-by-inhibiting-the-development-of-emt
#19
Jia Yang, Cheng-Zhi Zhou, Rui Zhu, Heng Fan, Xing-Xing Liu, Xue-Yun Duan, Qing Tang, Zhe-Xing Shou, Dong-Mei Zuo
BACKGROUND: Epithelial-mesenchymal transition (EMT), characterized by the decrease of E-Cad and increase of vimentin and α-SMA, was demonstrated to participate in inflammatory bowel disease (IBD) related fibrosis. MiR-200b plays an anti-fibrosis role in inhibiting EMT by targeting ZEB1 and ZEB2. But the stability of exogenous miR-200b in blood limits its application. Microvesicles (MVs), which can transfer miRNAs among cells and prevent them from degradation, may provide an excellent transport system for the delivery of miR-200b in the treatment of fibrosis...
March 31, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28369013/wound-healing-from-dermal-grafts-containing-cd34-cells-is-comparable-to-split-thickness-skin-micrografts
#20
Kristo Nuutila, Mansher Singh, Carla Kruse, Elof Eriksson
BACKGROUND: Epidermal stem cells present in the skin appendages of the dermis might be crucial in wound healing. In this study we located these cells in the dermis and evaluated their contribution to full-thickness wound healing in a porcine model. METHODS: Four sequentially deeper 0.35mm thick skin grafts were harvested from the same donor site going down to 1.4 mm in depth (Layers 1-4). The layers were minced to 0.8 x 0.8 x 0.35 mm micrografts and transplanted (1:2) onto full-thickness porcine wounds...
March 31, 2017: Plastic and Reconstructive Surgery
keyword
keyword
28999
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"